AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1333500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1333500 MtrunA17_Chr4g0065621 92.174 345 8 6 1 342 1 329 0.0 585
Msa1333500 MtrunA17_Chr4g0065651 77.034 553 73 10 1 540 1 512 8.65e-134 398
Msa1333500 MtrunA17_Chr0c22g0493881 79.653 403 41 6 1 399 1 366 8.88e-91 283
Msa1333500 MtrunA17_Chr2g0287751 66.795 259 58 10 1 253 1 237 9.43e-59 202
Msa1333500 MtrunA17_Chr2g0287741 46.619 562 176 24 10 540 15 483 4.24e-44 164
Msa1333500 MtrunA17_Chr4g0065611 91.603 131 10 1 411 540 12 142 1.23e-40 143
Msa1333500 MtrunA17_Chr0c22g0493871 91.538 130 10 1 412 540 1 130 3.78e-40 142
Msa1333500 MtrunA17_Chr1g0191671 59.559 136 50 3 1 131 1 136 4.83e-33 133
Msa1333500 MtrunA17_Chr1g0208301 70.513 78 20 1 1 75 1 78 1.00e-29 120
Msa1333500 MtrunA17_Chr7g0269081 67.143 70 23 0 5 74 17 86 2.80e-24 103
Msa1333500 MtrunA17_Chr4g0067291 42.568 148 59 3 7 128 71 218 6.38e-24 103
Msa1333500 MtrunA17_Chr3g0111541 53.521 71 33 0 3 73 5 75 4.82e-19 85.9
Msa1333500 MtrunA17_Chr1g0192131 57.333 75 31 1 4 77 9 83 7.00e-19 89.0
Msa1333500 MtrunA17_Chr7g0272571 53.521 71 33 0 9 79 32 102 1.39e-18 87.4
Msa1333500 MtrunA17_Chr2g0296001 60.294 68 27 0 7 74 70 137 4.45e-17 82.8
Msa1333500 MtrunA17_Chr1g0198341 39.189 74 45 0 10 83 4 77 1.77e-13 65.9
Msa1333500 MtrunA17_Chr8g0382721 52.459 61 29 0 14 74 33 93 7.93e-13 66.2
Msa1333500 MtrunA17_Chr1g0160201 45.902 61 33 0 14 74 29 89 1.62e-12 65.5
Msa1333500 MtrunA17_Chr2g0285561 46.575 73 38 1 8 79 6 78 2.11e-12 68.2
Msa1333500 MtrunA17_Chr3g0124411 47.761 67 35 0 8 74 28 94 2.54e-12 64.7
Msa1333500 MtrunA17_Chr2g0325601 42.254 71 41 0 14 84 6 76 3.71e-12 64.3
Msa1333500 MtrunA17_Chr1g0189221 37.975 79 49 0 1 79 44 122 3.91e-12 65.1
Msa1333500 MtrunA17_Chr7g0251491 33.654 104 62 3 2 98 40 143 7.59e-12 63.9
Msa1333500 MtrunA17_Chr4g0028461 48.276 87 43 2 12 96 68 154 1.01e-11 66.2
Msa1333500 MtrunA17_Chr7g0218981 37.037 81 51 0 13 93 7 87 1.45e-11 62.0
Msa1333500 MtrunA17_Chr5g0407821 37.179 78 49 0 3 80 16 93 1.79e-11 62.4
Msa1333500 MtrunA17_Chr5g0404641 41.176 68 40 0 9 76 2 69 5.97e-11 61.2
Msa1333500 MtrunA17_Chr4g0050571 45.455 66 35 1 10 75 101 165 9.79e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1333500 AT3G06130.2 55.109 274 104 5 1 274 1 255 5.48e-47 167
Msa1333500 AT5G19090.2 70.000 120 27 1 1 111 1 120 1.94e-43 161
Msa1333500 AT5G19090.3 70.000 120 27 1 1 111 1 120 1.94e-43 161
Msa1333500 AT5G19090.5 77.228 101 23 0 1 101 1 101 1.22e-42 161
Msa1333500 AT5G19090.4 77.228 101 23 0 1 101 1 101 1.22e-42 161
Msa1333500 AT5G19090.1 77.228 101 23 0 1 101 1 101 1.22e-42 161
Msa1333500 AT3G05220.1 63.514 74 27 0 1 74 1 74 2.84e-26 113
Msa1333500 AT5G27690.1 52.475 101 43 1 5 105 25 120 3.17e-26 110
Msa1333500 AT1G23000.1 61.628 86 29 2 1 83 1 85 1.09e-25 108
Msa1333500 AT1G23000.2 61.628 86 29 2 1 83 61 145 3.22e-25 108
Msa1333500 AT5G37860.1 54.412 68 31 0 9 76 9 76 2.77e-19 88.2
Msa1333500 AT1G56210.1 54.667 75 33 1 10 83 40 114 1.54e-18 87.8
Msa1333500 AT3G06130.1 50.382 131 22 6 435 540 361 473 2.33e-14 75.9
Msa1333500 AT1G06330.1 46.875 64 34 0 14 77 6 69 2.73e-13 68.2
Msa1333500 AT4G10465.1 36.957 92 58 0 1 92 42 133 4.07e-12 65.5
Msa1333500 AT1G66240.1 40.000 65 39 0 10 74 33 97 5.18e-12 63.2
Msa1333500 AT3G56891.1 43.750 64 36 0 14 77 20 83 1.59e-11 63.5
Msa1333500 AT3G02960.1 41.892 74 42 1 3 75 119 192 2.86e-11 64.3
Msa1333500 AT4G23882.1 43.678 87 38 3 9 84 77 163 4.17e-11 64.3
Msa1333500 AT3G56240.3 41.791 67 39 0 10 76 3 69 5.01e-11 60.8
Msa1333500 AT3G56240.1 41.791 67 39 0 10 76 3 69 5.01e-11 60.8
Msa1333500 AT1G71050.1 39.394 66 40 0 9 74 27 92 7.93e-11 60.8

Find 193 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGAAAGGTCACGGTTTC+AGG 0.104424 8_4:-53658487 Msa1333500:CDS
CAAGATCTAGCTCAATTTAA+AGG 0.182916 8_4:-53658142 Msa1333500:CDS
TAGGATTCTGATTCGGGTTC+TGG 0.245506 8_4:+53658089 None:intergenic
ACTTGTGATAAATAAAGATT+TGG 0.258061 8_4:+53658945 None:intergenic
GGATTACCCGACATCATTTC+CGG 0.260282 8_4:+53657263 None:intergenic
AAGAAGCAAGTTTGAGAGTT+AGG 0.269593 8_4:+53659110 None:intergenic
TATTGTTTCCACCACCCTTC+TGG 0.290425 8_4:+53658281 None:intergenic
CATCTTTAGGATTCTGATTC+GGG 0.295568 8_4:+53658083 None:intergenic
ATCAAGAAGCTTGCAAAATC+AGG 0.297726 8_4:-53658442 Msa1333500:CDS
TTAACACCTTTAGCATCTTT+AGG 0.299936 8_4:+53658070 None:intergenic
ACAACAACAGCAACAGTTAA+TGG 0.305860 8_4:-53657210 Msa1333500:CDS
CCCGACATCATTTCCGGGCT+TGG 0.308159 8_4:+53657269 None:intergenic
AGCTTCTTGATGAGAACATT+TGG 0.309355 8_4:+53658454 None:intergenic
GGAGAGAGAATGAGAAATAA+AGG 0.309923 8_4:+53659346 None:intergenic
TTGTATTTGTTGACCACCTT+TGG 0.313268 8_4:+53658252 None:intergenic
GTTACAAACTTTATTCCTAT+TGG 0.314373 8_4:-53658924 Msa1333500:five_prime_UTR
CCATTCCCCATAGGCATAGA+AGG 0.318053 8_4:+53657908 None:intergenic
CTTCTTTACTCATGACCAAT+AGG 0.330043 8_4:+53658909 None:intergenic
TAGTAGTCATTGAGAGAGAA+TGG 0.336882 8_4:+53659154 None:intergenic
GCATCTTTAGGATTCTGATT+CGG 0.340947 8_4:+53658082 None:intergenic
TTCTGGCCCGGGTGCTGATT+CGG 0.342924 8_4:+53658106 None:intergenic
TCTGATTCGGGTTCTGGCCC+GGG 0.343572 8_4:+53658095 None:intergenic
ACTGATTAACAACGTTGTTC+TGG 0.344905 8_4:+53658365 None:intergenic
GGTGATTATAGATCTGAAAA+TGG 0.348415 8_4:+53658983 None:intergenic
TTCTGATTCGGGTTCTGGCC+CGG 0.355308 8_4:+53658094 None:intergenic
AATGGCGGTGGTGGCGGAAA+CGG 0.358961 8_4:-53657821 Msa1333500:CDS
GTGGCCACCGGCGTCATTCT+CGG 0.359701 8_4:-53659243 Msa1333500:five_prime_UTR
AAGAAAGGTGGCGGCGGTGG+TGG 0.361940 8_4:-53657653 Msa1333500:CDS
TTCTATAAACTACTCCTCTA+TGG 0.368659 8_4:+53656833 None:intergenic
TAGAATGAAATGAGAATAGA+AGG 0.368945 8_4:+53659046 None:intergenic
CTAAAATCATCCTCATCTTC+AGG 0.371343 8_4:+53658037 None:intergenic
TTCAAATTCATGGCATGAAT+GGG 0.375304 8_4:-53657694 Msa1333500:CDS
ATGGCGGTGGTGGCGGAAAC+GGG 0.382069 8_4:-53657820 Msa1333500:CDS
AAGGAAAAGAGAGAAATAAA+TGG 0.382972 8_4:+53659065 None:intergenic
TAAGGATCTGGAGGAGGATA+AGG 0.389057 8_4:+53657071 None:intergenic
AGAGTTAGGAAGAGAGAGAA+TGG 0.398540 8_4:+53659124 None:intergenic
TGATGTTGCTGTTGTTGATA+AGG 0.399168 8_4:+53657242 None:intergenic
CTACCATTGCAGCACGTTGT+TGG 0.399981 8_4:+53657171 None:intergenic
CATCAAACAAACAATGTTCA+TGG 0.400654 8_4:-53656997 Msa1333500:three_prime_UTR
GGTAACATTTCTGCTGTTCA+AGG 0.405632 8_4:-53657332 Msa1333500:CDS
CAGAAATGTTACCACCCATT+TGG 0.407941 8_4:+53657342 None:intergenic
AATATATTGCATAAAATTAG+AGG 0.409097 8_4:+53656944 None:intergenic
GATTACCCGACATCATTTCC+GGG 0.409185 8_4:+53657264 None:intergenic
TTCTCTCTCACTAACACACT+TGG 0.409730 8_4:-53659204 Msa1333500:five_prime_UTR
GCAATAATCAAAATAACAAT+GGG 0.412169 8_4:-53657523 Msa1333500:CDS
GGAAAGAAAGGTGGCGGCGG+TGG 0.412415 8_4:-53657656 Msa1333500:CDS
CAGGGAAACATGCTGAGTTA+TGG 0.418710 8_4:-53658423 Msa1333500:CDS
AAAGGTGGCGGCGGTGGTGG+AGG 0.419490 8_4:-53657650 Msa1333500:CDS
GCTTCTTGATGAGAACATTT+GGG 0.423822 8_4:+53658455 None:intergenic
CCTGTGCCAGTTCAAATTCA+TGG 0.427491 8_4:-53657704 Msa1333500:CDS
TGTATTTGTTGACCACCTTT+GGG 0.427960 8_4:+53658253 None:intergenic
AAAATCTTACAGAAAATTGA+TGG 0.430085 8_4:-53658679 Msa1333500:intron
CCTGTGCCGGTTCAAATTCA+TGG 0.435135 8_4:-53657776 Msa1333500:CDS
AGTAGTCATTGAGAGAGAAT+GGG 0.436778 8_4:+53659155 None:intergenic
GGAAAGAAAGGTGGTGGTGG+TGG 0.438004 8_4:-53657728 Msa1333500:CDS
TTCATGCCATGAATTTGAAC+CGG 0.443413 8_4:+53657770 None:intergenic
ACGCCGGTGGCCACCGTCGC+CGG 0.451894 8_4:+53659252 None:intergenic
CTTGTGATAAATAAAGATTT+GGG 0.452079 8_4:+53658946 None:intergenic
GTTCAAATTCATGGCATGAA+TGG 0.453332 8_4:-53657695 Msa1333500:CDS
TAATACCACTGAGATCTTAC+CGG 0.456960 8_4:-53659271 Msa1333500:five_prime_UTR
AAAGCATGGCTGTAAGGATC+TGG 0.457940 8_4:+53657059 None:intergenic
CCTTCTATGCCTATGGGGAA+TGG 0.458052 8_4:-53657908 Msa1333500:CDS
AAAATGATGGGCGGCGGAGG+TGG 0.460753 8_4:-53657428 Msa1333500:CDS
TGATGAGTTTGATGATGAAA+TGG 0.461887 8_4:-53657960 Msa1333500:CDS
AACAACTTCAACAGATGAAA+GGG 0.462139 8_4:-53658168 Msa1333500:CDS
ACAGCTTATGAGTGCTATGA+AGG 0.465021 8_4:-53657855 Msa1333500:CDS
CATGGCATGAATGGTGGAAA+TGG 0.469268 8_4:-53657758 Msa1333500:CDS
TTCATGCCATGAATTTGAAC+TGG 0.469656 8_4:+53657698 None:intergenic
GCTGTTCAAGGCCTACCGGC+TGG 0.474751 8_4:-53657320 Msa1333500:CDS
TTCTGCTGTTCAAGGCCTAC+CGG 0.475565 8_4:-53657324 Msa1333500:CDS
ATTGACAATGGCAAAGGTGG+TGG 0.482824 8_4:-53658328 Msa1333500:CDS
AGAATGAGAAATAAAGGAAA+GGG 0.483580 8_4:+53659352 None:intergenic
GGCGGAAACGGGCAGAAACC+TGG 0.484298 8_4:-53657809 Msa1333500:CDS
TGGTTCATTCGCATACTCAT+TGG 0.484892 8_4:+53657362 None:intergenic
CACCCTGCTATGAGTGGTGC+AGG 0.485466 8_4:-53657398 Msa1333500:CDS
TCACTGAAAGCATGGCTGTA+AGG 0.487097 8_4:+53657053 None:intergenic
AAGGATCTGGAGGAGGATAA+GGG 0.490431 8_4:+53657072 None:intergenic
TTACCACCACTACCAGCAAT+TGG 0.502799 8_4:+53657479 None:intergenic
GGTGGCCTTGCATACATCAT+TGG 0.504586 8_4:+53657125 None:intergenic
TCTGGAGGAGGATAAGGGTA+AGG 0.506412 8_4:+53657077 None:intergenic
GGTATGTCGATACCAATTGC+TGG 0.507057 8_4:-53657491 Msa1333500:CDS
AGCAATAATCAAAATAACAA+TGG 0.513280 8_4:-53657524 Msa1333500:CDS
AAGGAAAGGTCACGGTTTCA+GGG 0.515226 8_4:-53658486 Msa1333500:CDS
TTACCTGCACCACTCATAGC+AGG 0.515328 8_4:+53657395 None:intergenic
CCATGAATTTGAACCGGCAC+AGG 0.516132 8_4:+53657776 None:intergenic
AATGGTAATGGAGGAAAGAA+AGG 0.520568 8_4:-53657668 Msa1333500:CDS
CAGAATCCTAAAGATGCTAA+AGG 0.521196 8_4:-53658076 Msa1333500:CDS
GATGCCGCCGAATCAGCACC+CGG 0.521854 8_4:-53658113 Msa1333500:CDS
AATCAAAACCAGAAGGGTGG+TGG 0.522342 8_4:-53658289 Msa1333500:CDS
GGAGGAAAGAAAGGTGGTGG+TGG 0.522847 8_4:-53657731 Msa1333500:CDS
GGTGCTCACATGATGTTGAA+TGG 0.526053 8_4:-53657887 Msa1333500:CDS
GTTCAAATTCATGGCATGAA+TGG 0.526661 8_4:-53657767 Msa1333500:CDS
CAACAACTTCAACAGATGAA+AGG 0.526664 8_4:-53658169 Msa1333500:CDS
GCTGGCGGAGGATACTTTCA+AGG 0.526671 8_4:-53657302 Msa1333500:CDS
CAAGGCCTACCGGCTGGCGG+AGG 0.527853 8_4:-53657314 Msa1333500:CDS
AACGGGCAGAAACCTGGTGG+TGG 0.531908 8_4:-53657803 Msa1333500:CDS
AATGGCAATGGAGGAAAGAA+AGG 0.535264 8_4:-53657740 Msa1333500:CDS
AGGTGGTGGTGGTCCAAGCC+CGG 0.536761 8_4:-53657282 Msa1333500:CDS
GAGAATGAGAAATAAAGGAA+AGG 0.536809 8_4:+53659351 None:intergenic
GAGTATGCGAATGAACCAAA+TGG 0.538895 8_4:-53657357 Msa1333500:CDS
CCATGAATTTGAACTGGCAC+AGG 0.538960 8_4:+53657704 None:intergenic
TTACCGGCGACGGTGGCCAC+CGG 0.539537 8_4:-53659255 Msa1333500:five_prime_UTR
GGTGGTGGAGGAAACAAGAA+TGG 0.539555 8_4:-53657560 Msa1333500:CDS
GTGCTCACATGATGTTGAAT+GGG 0.541270 8_4:-53657886 Msa1333500:CDS
GGATACTTTCAAGGTGGTGG+TGG 0.542661 8_4:-53657293 Msa1333500:CDS
CCAAGCCCGGAAATGATGTC+GGG 0.544393 8_4:-53657269 Msa1333500:CDS
TGAAAGTATCCTCCGCCAGC+CGG 0.545772 8_4:+53657305 None:intergenic
GAATCACATGACATTGCAGC+TGG 0.546046 8_4:+53657019 None:intergenic
GGAGGAGGATAAGGGTAAGG+AGG 0.549476 8_4:+53657080 None:intergenic
GGGCAGAAACCTGGTGGTGG+AGG 0.550293 8_4:-53657800 Msa1333500:CDS
TCAAGAAGCTTGCAAAATCA+GGG 0.551852 8_4:-53658441 Msa1333500:CDS
GGAGGATACTTTCAAGGTGG+TGG 0.554853 8_4:-53657296 Msa1333500:CDS
TGGTGGTGGAGGACCTGTGC+CGG 0.556236 8_4:-53657789 Msa1333500:CDS
GTAGTCATTGAGAGAGAATG+GGG 0.559844 8_4:+53659156 None:intergenic
ATGTAATTCACAGCAGGAGG+TGG 0.568435 8_4:+53657107 None:intergenic
CAAATTCATGGCATGAATGG+TGG 0.570734 8_4:-53657764 Msa1333500:CDS
CATTCCCCATAGGCATAGAA+GGG 0.572536 8_4:+53657909 None:intergenic
AATAATCAGAATCACGGTGG+AGG 0.574664 8_4:-53657626 Msa1333500:CDS
TTTGTTGACCACCTTTGGGT+TGG 0.576873 8_4:+53658257 None:intergenic
GGGTACATGTAATTCACAGC+AGG 0.576931 8_4:+53657101 None:intergenic
GGCACAGGTCCTCCACCACC+AGG 0.577295 8_4:+53657791 None:intergenic
AATGTTCAAGCTATGAACAA+TGG 0.581859 8_4:-53657458 Msa1333500:CDS
GAGTTTGATGATGAAATGGT+TGG 0.582338 8_4:-53657956 Msa1333500:CDS
TCTCTCTCACTAACACACTT+GGG 0.583240 8_4:-53659203 Msa1333500:five_prime_UTR
TCCAAGCCCGGAAATGATGT+CGG 0.584579 8_4:-53657270 Msa1333500:CDS
GGAGGAAAGAAAGGTGGCGG+CGG 0.585808 8_4:-53657659 Msa1333500:CDS
AGCAGCCAATGATGTATGCA+AGG 0.586173 8_4:-53657130 Msa1333500:CDS
TCGATACCAATTGCTGGTAG+TGG 0.586867 8_4:-53657485 Msa1333500:CDS
ACAAACAATGTTCATGGTGA+TGG 0.588045 8_4:-53656991 Msa1333500:three_prime_UTR
ATGAATGGTGGAAATGGCAA+TGG 0.589322 8_4:-53657752 Msa1333500:CDS
GTTCAAGGCCTACCGGCTGG+CGG 0.593786 8_4:-53657317 Msa1333500:CDS
AGTATCCTCCGCCAGCCGGT+AGG 0.594256 8_4:+53657309 None:intergenic
ATATATTGCATAAAATTAGA+GGG 0.595405 8_4:+53656945 None:intergenic
CATCCTCACCCTGCTATGAG+TGG 0.595605 8_4:-53657404 Msa1333500:CDS
TGGCTGTAAGGATCTGGAGG+AGG 0.596052 8_4:+53657065 None:intergenic
CAACAAATACAACAACTACA+AGG 0.597690 8_4:-53658241 Msa1333500:CDS
AGGTCAGAATCAAAACCAGA+AGG 0.597938 8_4:-53658296 Msa1333500:CDS
ATTTGATGTGCCTGAAGATG+AGG 0.599659 8_4:-53658047 Msa1333500:CDS
TCAAATTCATGGCATGAATG+GGG 0.600577 8_4:-53657693 Msa1333500:CDS
ATTGCATAAAATTAGAGGGT+GGG 0.603462 8_4:+53656949 None:intergenic
GGCAATGGAGGAAAGAAAGG+TGG 0.603629 8_4:-53657737 Msa1333500:CDS
AACAATAACCAACCCAAAGG+TGG 0.605261 8_4:-53658265 Msa1333500:CDS
AATGGAGGAAAGAAAGGTGG+TGG 0.606743 8_4:-53657734 Msa1333500:CDS
GATAGATGCAGAACAAGGAA+AGG 0.608149 8_4:-53658500 Msa1333500:CDS
ATACCAATTGCTGGTAGTGG+TGG 0.608621 8_4:-53657482 Msa1333500:CDS
TATTGCATAAAATTAGAGGG+TGG 0.609150 8_4:+53656948 None:intergenic
CAAATTGACAATGGCAAAGG+TGG 0.610618 8_4:-53658331 Msa1333500:CDS
AGTATGCGAATGAACCAAAT+GGG 0.610690 8_4:-53657356 Msa1333500:CDS
TTCATAGCACTCATAAGCTG+TGG 0.610940 8_4:+53657857 None:intergenic
ATGCAAATTGACAATGGCAA+AGG 0.611165 8_4:-53658334 Msa1333500:CDS
CAAATTCATGGCATGAATGG+GGG 0.612639 8_4:-53657692 Msa1333500:CDS
GGTGGAGGAAACAAGAATGG+AGG 0.612726 8_4:-53657557 Msa1333500:CDS
CACTGAGATCTTACCGGCGA+CGG 0.616024 8_4:-53659265 Msa1333500:five_prime_UTR
AAAGTGAACATACACTGTGA+TGG 0.617847 8_4:-53658721 Msa1333500:CDS
ATGTGAGCACCATTCCCCAT+AGG 0.619841 8_4:+53657899 None:intergenic
GGGGTTTCTAATTACCATAG+AGG 0.620908 8_4:-53656847 Msa1333500:three_prime_UTR
GGTCAGAATCAAAACCAGAA+GGG 0.626233 8_4:-53658295 Msa1333500:CDS
GGTCACGGTTTCAGGGAATG+TGG 0.626243 8_4:-53658479 Msa1333500:CDS
TGGCCCGGGTGCTGATTCGG+CGG 0.627521 8_4:+53658109 None:intergenic
AAGAACATGCAAATTGACAA+TGG 0.628311 8_4:-53658340 Msa1333500:CDS
GAGGAGGATAAGGGTAAGGA+GGG 0.628873 8_4:+53657081 None:intergenic
GCACCACTCATAGCAGGGTG+AGG 0.629783 8_4:+53657401 None:intergenic
GAACCAACAACGTGCTGCAA+TGG 0.631234 8_4:-53657174 Msa1333500:CDS
AATGGAGGAAAGAAAGGTGG+CGG 0.631313 8_4:-53657662 Msa1333500:CDS
GCATGGCTGTAAGGATCTGG+AGG 0.631436 8_4:+53657062 None:intergenic
TGAGATCTTACCGGCGACGG+TGG 0.634936 8_4:-53659262 Msa1333500:five_prime_UTR
CTTATGAGTGCTATGAAGGG+TGG 0.638762 8_4:-53657851 Msa1333500:CDS
GGAGGAAATAATCAGAATCA+CGG 0.638839 8_4:-53657632 Msa1333500:CDS
TGCAGAACAAGGAAAGGTCA+CGG 0.641237 8_4:-53658494 Msa1333500:CDS
AATAATCAAAATAACAATGG+GGG 0.641757 8_4:-53657521 Msa1333500:CDS
CAGCTTATGAGTGCTATGAA+GGG 0.642847 8_4:-53657854 Msa1333500:CDS
ATGCCGCCGAATCAGCACCC+GGG 0.644621 8_4:-53658112 Msa1333500:CDS
ACTGAGATAGATGCAGAACA+AGG 0.647644 8_4:-53658505 Msa1333500:CDS
TACATGTAATTCACAGCAGG+AGG 0.650237 8_4:+53657104 None:intergenic
GGTAATGGAGGAAAGAAAGG+TGG 0.651222 8_4:-53657665 Msa1333500:CDS
AGAGAGACCGAGAATGACGC+CGG 0.658961 8_4:+53659236 None:intergenic
TACCTGCACCACTCATAGCA+GGG 0.659944 8_4:+53657396 None:intergenic
GAGTGGTGCAGGTAATAATG+TGG 0.661904 8_4:-53657387 Msa1333500:CDS
AATGGTGGAAATGGCAATGG+AGG 0.665518 8_4:-53657749 Msa1333500:CDS
CAATAATCAAAATAACAATG+GGG 0.668955 8_4:-53657522 Msa1333500:CDS
ATTCCCCATAGGCATAGAAG+GGG 0.672578 8_4:+53657910 None:intergenic
GAGACCGAGAATGACGCCGG+TGG 0.677480 8_4:+53659239 None:intergenic
AAAGGTGGTGGAAACAACAA+AGG 0.677851 8_4:-53658316 Msa1333500:CDS
CAGAATCAAAACCAGAAGGG+TGG 0.682186 8_4:-53658292 Msa1333500:CDS
GGAAACAATAACCAACCCAA+AGG 0.682874 8_4:-53658268 Msa1333500:CDS
ATGCGAATGAACCAAATGGG+TGG 0.690349 8_4:-53657353 Msa1333500:CDS
GGAAACGGGCAGAAACCTGG+TGG 0.691525 8_4:-53657806 Msa1333500:CDS
TTCCCCATAGGCATAGAAGG+GGG 0.698362 8_4:+53657911 None:intergenic
AGTGGTGCAGGTAATAATGT+GGG 0.701968 8_4:-53657386 Msa1333500:CDS
AAATAGCGGTGGTTTACCGG+AGG 0.703021 8_4:-53657597 Msa1333500:CDS
GGCGGAGGATACTTTCAAGG+TGG 0.717626 8_4:-53657299 Msa1333500:CDS
GGAAATAATCAGAATCACGG+TGG 0.727521 8_4:-53657629 Msa1333500:CDS
CGTCGCCGGTAAGATCTCAG+TGG 0.738206 8_4:+53659266 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATTTTGATTATTGTT+TGG + chr8_4:53657744-53657763 None:intergenic 10.0%
!! ATAATTGAAAGCTATAATTA+AGG + chr8_4:53657513-53657532 None:intergenic 15.0%
!! ATATATTGCATAAAATTAGA+GGG + chr8_4:53659187-53659206 None:intergenic 15.0%
!! AATATATTGCATAAAATTAG+AGG + chr8_4:53659188-53659207 None:intergenic 15.0%
!! CTTGTGATAAATAAAGATTT+GGG + chr8_4:53657186-53657205 None:intergenic 20.0%
!! ACTTGTGATAAATAAAGATT+TGG + chr8_4:53657187-53657206 None:intergenic 20.0%
!! CAAAAAAAGATAAAGAAACA+TGG + chr8_4:53657281-53657300 None:intergenic 20.0%
!! TCTGCATACAAAAAAAAATA+AGG + chr8_4:53657377-53657396 None:intergenic 20.0%
!! AAAATCTTACAGAAAATTGA+TGG - chr8_4:53657450-53657469 Msa1333500:CDS 20.0%
!! AACATTTACTCTCAAAATTA+AGG + chr8_4:53657559-53657578 None:intergenic 20.0%
!!! TTTTGATTATTGTTTGGTTT+GGG + chr8_4:53657738-53657757 None:intergenic 20.0%
!!! ATTTTGATTATTGTTTGGTT+TGG + chr8_4:53657739-53657758 None:intergenic 20.0%
!! AGCAATAATCAAAATAACAA+TGG - chr8_4:53658605-53658624 Msa1333500:intron 20.0%
!! GCAATAATCAAAATAACAAT+GGG - chr8_4:53658606-53658625 Msa1333500:intron 20.0%
!! CAATAATCAAAATAACAATG+GGG - chr8_4:53658607-53658626 Msa1333500:intron 20.0%
!! AATAATCAAAATAACAATGG+GGG - chr8_4:53658608-53658627 Msa1333500:intron 20.0%
!!! AATGGATTTCAAAAAATGAT+GGG - chr8_4:53658689-53658708 Msa1333500:CDS 20.0%
!! TAAAAAACTCATGTTTCATT+GGG + chr8_4:53659214-53659233 None:intergenic 20.0%
!!! ACTATGTTTATTTTGTTCTT+TGG - chr8_4:53659336-53659355 Msa1333500:five_prime_UTR 20.0%
! AGAATGAGAAATAAAGGAAA+GGG + chr8_4:53656780-53656799 None:intergenic 25.0%
! AAGGAAAAGAGAGAAATAAA+TGG + chr8_4:53657067-53657086 None:intergenic 25.0%
! TAGAATGAAATGAGAATAGA+AGG + chr8_4:53657086-53657105 None:intergenic 25.0%
!!! TATTTTATGTTCTTGTTTGG+TGG + chr8_4:53657124-53657143 None:intergenic 25.0%
!! GAGTATTTTATGTTCTTGTT+TGG + chr8_4:53657127-53657146 None:intergenic 25.0%
! GTTACAAACTTTATTCCTAT+TGG - chr8_4:53657205-53657224 Msa1333500:CDS 25.0%
!!! AGAAGAATTTTTGAAGATAC+AGG - chr8_4:53657238-53657257 Msa1333500:CDS 25.0%
!! TCAAAATTAAGGTTCTGTTT+TGG + chr8_4:53657548-53657567 None:intergenic 25.0%
!!! TGATTTTAGTGATGATGAAA+TGG - chr8_4:53658106-53658125 Msa1333500:CDS 25.0%
!! CAATGGATTTCAAAAAATGA+TGG - chr8_4:53658688-53658707 Msa1333500:CDS 25.0%
! CTAAAAAACTCATGTTTCAT+TGG + chr8_4:53659215-53659234 None:intergenic 25.0%
GAGAATGAGAAATAAAGGAA+AGG + chr8_4:53656781-53656800 None:intergenic 30.0%
!!! TTGTTTGGTGGAGTTTTATT+TGG + chr8_4:53657112-53657131 None:intergenic 30.0%
GGTGATTATAGATCTGAAAA+TGG + chr8_4:53657149-53657168 None:intergenic 30.0%
ATTTGGGCTATAGAGAAAAA+TGG + chr8_4:53657170-53657189 None:intergenic 30.0%
!! TGTGTTGATTAATGAATGAC+AGG - chr8_4:53657591-53657610 Msa1333500:CDS 30.0%
AAGAACATGCAAATTGACAA+TGG - chr8_4:53657789-53657808 Msa1333500:CDS 30.0%
CAACAAATACAACAACTACA+AGG - chr8_4:53657888-53657907 Msa1333500:CDS 30.0%
AACAACTTCAACAGATGAAA+GGG - chr8_4:53657961-53657980 Msa1333500:CDS 30.0%
! CAAGATCTAGCTCAATTTAA+AGG - chr8_4:53657987-53658006 Msa1333500:CDS 30.0%
TTAACACCTTTAGCATCTTT+AGG + chr8_4:53658062-53658081 None:intergenic 30.0%
!! TGATGAGTTTGATGATGAAA+TGG - chr8_4:53658169-53658188 Msa1333500:CDS 30.0%
TTCAAATTCATGGCATGAAT+GGG - chr8_4:53658363-53658382 Msa1333500:CDS 30.0%
AAATAACAATGGGGGTAAAA+AGG - chr8_4:53658616-53658635 Msa1333500:intron 30.0%
AATAACAATGGGGGTAAAAA+GGG - chr8_4:53658617-53658636 Msa1333500:intron 30.0%
AATGTTCAAGCTATGAACAA+TGG - chr8_4:53658671-53658690 Msa1333500:intron 30.0%
CATCAAACAAACAATGTTCA+TGG - chr8_4:53659132-53659151 Msa1333500:five_prime_UTR 30.0%
ATTGCATAAAATTAGAGGGT+GGG + chr8_4:53659183-53659202 None:intergenic 30.0%
TATTGCATAAAATTAGAGGG+TGG + chr8_4:53659184-53659203 None:intergenic 30.0%
TTCTATAAACTACTCCTCTA+TGG + chr8_4:53659299-53659318 None:intergenic 30.0%
GGAGAGAGAATGAGAAATAA+AGG + chr8_4:53656786-53656805 None:intergenic 35.0%
TAATACCACTGAGATCTTAC+CGG - chr8_4:53656858-53656877 Msa1333500:three_prime_UTR 35.0%
AGTAGTCATTGAGAGAGAAT+GGG + chr8_4:53656977-53656996 None:intergenic 35.0%
TAGTAGTCATTGAGAGAGAA+TGG + chr8_4:53656978-53656997 None:intergenic 35.0%
AAGAAGCAAGTTTGAGAGTT+AGG + chr8_4:53657022-53657041 None:intergenic 35.0%
CTTCTTTACTCATGACCAAT+AGG + chr8_4:53657223-53657242 None:intergenic 35.0%
AAAGTGAACATACACTGTGA+TGG - chr8_4:53657408-53657427 Msa1333500:CDS 35.0%
GCTTCTTGATGAGAACATTT+GGG + chr8_4:53657677-53657696 None:intergenic 35.0%
AGCTTCTTGATGAGAACATT+TGG + chr8_4:53657678-53657697 None:intergenic 35.0%
ATCAAGAAGCTTGCAAAATC+AGG - chr8_4:53657687-53657706 Msa1333500:CDS 35.0%
TCAAGAAGCTTGCAAAATCA+GGG - chr8_4:53657688-53657707 Msa1333500:CDS 35.0%
ACTGATTAACAACGTTGTTC+TGG + chr8_4:53657767-53657786 None:intergenic 35.0%
ATGCAAATTGACAATGGCAA+AGG - chr8_4:53657795-53657814 Msa1333500:CDS 35.0%
! TGTATTTGTTGACCACCTTT+GGG + chr8_4:53657879-53657898 None:intergenic 35.0%
! TTGTATTTGTTGACCACCTT+TGG + chr8_4:53657880-53657899 None:intergenic 35.0%
CAACAACTTCAACAGATGAA+AGG - chr8_4:53657960-53657979 Msa1333500:CDS 35.0%
!! CATCTTTAGGATTCTGATTC+GGG + chr8_4:53658049-53658068 None:intergenic 35.0%
!! GCATCTTTAGGATTCTGATT+CGG + chr8_4:53658050-53658069 None:intergenic 35.0%
CAGAATCCTAAAGATGCTAA+AGG - chr8_4:53658053-53658072 Msa1333500:CDS 35.0%
CTAAAATCATCCTCATCTTC+AGG + chr8_4:53658095-53658114 None:intergenic 35.0%
!! GAGTTTGATGATGAAATGGT+TGG - chr8_4:53658173-53658192 Msa1333500:CDS 35.0%
! ATGAAGGGTGGCAATTTTAA+TGG - chr8_4:53658290-53658309 Msa1333500:CDS 35.0%
TTCATGCCATGAATTTGAAC+CGG + chr8_4:53658362-53658381 None:intergenic 35.0%
GTTCAAATTCATGGCATGAA+TGG - chr8_4:53658362-53658381 Msa1333500:CDS 35.0%
TTCATGCCATGAATTTGAAC+TGG + chr8_4:53658362-53658381 None:intergenic 35.0%
GTTCAAATTCATGGCATGAA+TGG - chr8_4:53658362-53658381 Msa1333500:CDS 35.0%
TCAAATTCATGGCATGAATG+GGG - chr8_4:53658364-53658383 Msa1333500:CDS 35.0%
!! AATGGTAATGGAGGAAAGAA+AGG - chr8_4:53658461-53658480 Msa1333500:CDS 35.0%
GGAGGAAATAATCAGAATCA+CGG - chr8_4:53658497-53658516 Msa1333500:CDS 35.0%
! GGATTTCAAAAAATGATGGG+CGG - chr8_4:53658692-53658711 Msa1333500:CDS 35.0%
AGTATGCGAATGAACCAAAT+GGG - chr8_4:53658773-53658792 Msa1333500:intron 35.0%
! TGATGTTGCTGTTGTTGATA+AGG + chr8_4:53658890-53658909 None:intergenic 35.0%
ACAACAACAGCAACAGTTAA+TGG - chr8_4:53658919-53658938 Msa1333500:exon 35.0%
! TGTTTTCATCACTGAAAGCA+TGG + chr8_4:53659087-53659106 None:intergenic 35.0%
ACAAACAATGTTCATGGTGA+TGG - chr8_4:53659138-53659157 Msa1333500:five_prime_UTR 35.0%
! TTGATCCCCTTTTCATCTAT+AGG - chr8_4:53659260-53659279 Msa1333500:five_prime_UTR 35.0%
! TGATCCCCTTTTCATCTATA+GGG - chr8_4:53659261-53659280 Msa1333500:five_prime_UTR 35.0%
AAACCCCCTATAGATGAAAA+GGG + chr8_4:53659269-53659288 None:intergenic 35.0%
TTCTCTCTCACTAACACACT+TGG - chr8_4:53656925-53656944 Msa1333500:three_prime_UTR 40.0%
TCTCTCTCACTAACACACTT+GGG - chr8_4:53656926-53656945 Msa1333500:three_prime_UTR 40.0%
GTAGTCATTGAGAGAGAATG+GGG + chr8_4:53656976-53656995 None:intergenic 40.0%
AGAGTTAGGAAGAGAGAGAA+TGG + chr8_4:53657008-53657027 None:intergenic 40.0%
! AAGAAACATGGAGAGTGTGA+TGG + chr8_4:53657269-53657288 None:intergenic 40.0%
ACTGAGATAGATGCAGAACA+AGG - chr8_4:53657624-53657643 Msa1333500:CDS 40.0%
GATAGATGCAGAACAAGGAA+AGG - chr8_4:53657629-53657648 Msa1333500:CDS 40.0%
CAAATTGACAATGGCAAAGG+TGG - chr8_4:53657798-53657817 Msa1333500:CDS 40.0%
!! AAAGGTGGTGGAAACAACAA+AGG - chr8_4:53657813-53657832 Msa1333500:CDS 40.0%
AGGTCAGAATCAAAACCAGA+AGG - chr8_4:53657833-53657852 Msa1333500:CDS 40.0%
GGTCAGAATCAAAACCAGAA+GGG - chr8_4:53657834-53657853 Msa1333500:CDS 40.0%
GGAAACAATAACCAACCCAA+AGG - chr8_4:53657861-53657880 Msa1333500:CDS 40.0%
AACAATAACCAACCCAAAGG+TGG - chr8_4:53657864-53657883 Msa1333500:CDS 40.0%
ATTTGATGTGCCTGAAGATG+AGG - chr8_4:53658082-53658101 Msa1333500:CDS 40.0%
GAAGGGGGTTTCATCTTATT+AGG + chr8_4:53658206-53658225 None:intergenic 40.0%
GTGCTCACATGATGTTGAAT+GGG - chr8_4:53658243-53658262 Msa1333500:CDS 40.0%
TTCATAGCACTCATAAGCTG+TGG + chr8_4:53658275-53658294 None:intergenic 40.0%
ACAGCTTATGAGTGCTATGA+AGG - chr8_4:53658274-53658293 Msa1333500:CDS 40.0%
CAGCTTATGAGTGCTATGAA+GGG - chr8_4:53658275-53658294 Msa1333500:CDS 40.0%
!! AAGGGTGGCAATTTTAATGG+CGG - chr8_4:53658293-53658312 Msa1333500:CDS 40.0%
CAAATTCATGGCATGAATGG+TGG - chr8_4:53658365-53658384 Msa1333500:CDS 40.0%
! ATGAATGGTGGAAATGGCAA+TGG - chr8_4:53658377-53658396 Msa1333500:CDS 40.0%
AATGGCAATGGAGGAAAGAA+AGG - chr8_4:53658389-53658408 Msa1333500:CDS 40.0%
CAAATTCATGGCATGAATGG+GGG - chr8_4:53658365-53658384 Msa1333500:CDS 40.0%
ATGAATGGGGGAAATGGTAA+TGG - chr8_4:53658449-53658468 Msa1333500:CDS 40.0%
GGAAATAATCAGAATCACGG+TGG - chr8_4:53658500-53658519 Msa1333500:CDS 40.0%
AATAATCAGAATCACGGTGG+AGG - chr8_4:53658503-53658522 Msa1333500:CDS 40.0%
CAAAAATAGCGGTGGTTTAC+CGG - chr8_4:53658529-53658548 Msa1333500:intron 40.0%
!! TTTCAAAAAATGATGGGCGG+CGG - chr8_4:53658695-53658714 Msa1333500:CDS 40.0%
AGTGGTGCAGGTAATAATGT+GGG - chr8_4:53658743-53658762 Msa1333500:intron 40.0%
TGGTTCATTCGCATACTCAT+TGG + chr8_4:53658770-53658789 None:intergenic 40.0%
GAGTATGCGAATGAACCAAA+TGG - chr8_4:53658772-53658791 Msa1333500:intron 40.0%
CAGAAATGTTACCACCCATT+TGG + chr8_4:53658790-53658809 None:intergenic 40.0%
GGTAACATTTCTGCTGTTCA+AGG - chr8_4:53658797-53658816 Msa1333500:intron 40.0%
TACATGTAATTCACAGCAGG+AGG + chr8_4:53659028-53659047 None:intergenic 40.0%
! GATCCCCTTTTCATCTATAG+GGG - chr8_4:53659262-53659281 Msa1333500:five_prime_UTR 40.0%
! ATCCCCTTTTCATCTATAGG+GGG - chr8_4:53659263-53659282 Msa1333500:five_prime_UTR 40.0%
AACCCCCTATAGATGAAAAG+GGG + chr8_4:53659268-53659287 None:intergenic 40.0%
GAAACCCCCTATAGATGAAA+AGG + chr8_4:53659270-53659289 None:intergenic 40.0%
GGGGTTTCTAATTACCATAG+AGG - chr8_4:53659282-53659301 Msa1333500:five_prime_UTR 40.0%
TGCAGAACAAGGAAAGGTCA+CGG - chr8_4:53657635-53657654 Msa1333500:CDS 45.0%
AAGGAAAGGTCACGGTTTCA+GGG - chr8_4:53657643-53657662 Msa1333500:CDS 45.0%
CAGGGAAACATGCTGAGTTA+TGG - chr8_4:53657706-53657725 Msa1333500:CDS 45.0%
ATTGACAATGGCAAAGGTGG+TGG - chr8_4:53657801-53657820 Msa1333500:CDS 45.0%
CAGAATCAAAACCAGAAGGG+TGG - chr8_4:53657837-53657856 Msa1333500:CDS 45.0%
AATCAAAACCAGAAGGGTGG+TGG - chr8_4:53657840-53657859 Msa1333500:CDS 45.0%
TATTGTTTCCACCACCCTTC+TGG + chr8_4:53657851-53657870 None:intergenic 45.0%
TTTGTTGACCACCTTTGGGT+TGG + chr8_4:53657875-53657894 None:intergenic 45.0%
!! TAGGATTCTGATTCGGGTTC+TGG + chr8_4:53658043-53658062 None:intergenic 45.0%
GGGGGTTTCATCTTATTAGG+TGG + chr8_4:53658203-53658222 None:intergenic 45.0%
AAACCCCCTTCTATGCCTAT+GGG - chr8_4:53658215-53658234 Msa1333500:CDS 45.0%
ATTCCCCATAGGCATAGAAG+GGG + chr8_4:53658222-53658241 None:intergenic 45.0%
CATTCCCCATAGGCATAGAA+GGG + chr8_4:53658223-53658242 None:intergenic 45.0%
GGTGCTCACATGATGTTGAA+TGG - chr8_4:53658242-53658261 Msa1333500:CDS 45.0%
CTTATGAGTGCTATGAAGGG+TGG - chr8_4:53658278-53658297 Msa1333500:CDS 45.0%
!! AATTTTAATGGCGGTGGTGG+CGG - chr8_4:53658302-53658321 Msa1333500:CDS 45.0%
! CATGGCATGAATGGTGGAAA+TGG - chr8_4:53658371-53658390 Msa1333500:CDS 45.0%
! AATGGTGGAAATGGCAATGG+AGG - chr8_4:53658380-53658399 Msa1333500:CDS 45.0%
AATGGAGGAAAGAAAGGTGG+TGG - chr8_4:53658395-53658414 Msa1333500:CDS 45.0%
CCATGAATTTGAACTGGCAC+AGG + chr8_4:53658428-53658447 None:intergenic 45.0%
CCTGTGCCAGTTCAAATTCA+TGG - chr8_4:53658425-53658444 Msa1333500:CDS 45.0%
!! AATGGGGGAAATGGTAATGG+AGG - chr8_4:53658452-53658471 Msa1333500:CDS 45.0%
!! GGTAATGGAGGAAAGAAAGG+TGG - chr8_4:53658464-53658483 Msa1333500:CDS 45.0%
AATGGAGGAAAGAAAGGTGG+CGG - chr8_4:53658395-53658414 Msa1333500:CDS 45.0%
GGTGGAGGCAACAAAAATAG+CGG - chr8_4:53658518-53658537 Msa1333500:CDS 45.0%
GGTTTACCGGAGGCTAAAAA+TGG - chr8_4:53658542-53658561 Msa1333500:intron 45.0%
TTACCGGAGGCTAAAAATGG+TGG - chr8_4:53658545-53658564 Msa1333500:intron 45.0%
GGTATGTCGATACCAATTGC+TGG - chr8_4:53658638-53658657 Msa1333500:intron 45.0%
TCGATACCAATTGCTGGTAG+TGG - chr8_4:53658644-53658663 Msa1333500:intron 45.0%
!! ATACCAATTGCTGGTAGTGG+TGG - chr8_4:53658647-53658666 Msa1333500:intron 45.0%
TTACCACCACTACCAGCAAT+TGG + chr8_4:53658653-53658672 None:intergenic 45.0%
GAGTGGTGCAGGTAATAATG+TGG - chr8_4:53658742-53658761 Msa1333500:intron 45.0%
ATGCGAATGAACCAAATGGG+TGG - chr8_4:53658776-53658795 Msa1333500:intron 45.0%
GATTACCCGACATCATTTCC+GGG + chr8_4:53658868-53658887 None:intergenic 45.0%
GGATTACCCGACATCATTTC+CGG + chr8_4:53658869-53658888 None:intergenic 45.0%
AGCAGCCAATGATGTATGCA+AGG - chr8_4:53658999-53659018 Msa1333500:five_prime_UTR 45.0%
ATGTAATTCACAGCAGGAGG+TGG + chr8_4:53659025-53659044 None:intergenic 45.0%
GGGTACATGTAATTCACAGC+AGG + chr8_4:53659031-53659050 None:intergenic 45.0%
AAGGATCTGGAGGAGGATAA+GGG + chr8_4:53659060-53659079 None:intergenic 45.0%
TAAGGATCTGGAGGAGGATA+AGG + chr8_4:53659061-53659080 None:intergenic 45.0%
AAAGCATGGCTGTAAGGATC+TGG + chr8_4:53659073-53659092 None:intergenic 45.0%
! TCACTGAAAGCATGGCTGTA+AGG + chr8_4:53659079-53659098 None:intergenic 45.0%
GAATCACATGACATTGCAGC+TGG + chr8_4:53659113-53659132 None:intergenic 45.0%
AGAGAGAGAGAGAGTTGCAG+AGG + chr8_4:53656807-53656826 None:intergenic 50.0%
CAAGGAAAGGTCACGGTTTC+AGG - chr8_4:53657642-53657661 Msa1333500:CDS 50.0%
GAAACCCCCTTCTATGCCTA+TGG - chr8_4:53658214-53658233 Msa1333500:CDS 50.0%
AACCCCCTTCTATGCCTATG+GGG - chr8_4:53658216-53658235 Msa1333500:CDS 50.0%
TTCCCCATAGGCATAGAAGG+GGG + chr8_4:53658221-53658240 None:intergenic 50.0%
CCATTCCCCATAGGCATAGA+AGG + chr8_4:53658224-53658243 None:intergenic 50.0%
CCTTCTATGCCTATGGGGAA+TGG - chr8_4:53658221-53658240 Msa1333500:CDS 50.0%
ATGTGAGCACCATTCCCCAT+AGG + chr8_4:53658233-53658252 None:intergenic 50.0%
!! GGTGGCAATTTTAATGGCGG+TGG - chr8_4:53658296-53658315 Msa1333500:CDS 50.0%
!! GGCAATTTTAATGGCGGTGG+TGG - chr8_4:53658299-53658318 Msa1333500:CDS 50.0%
CCATGAATTTGAACCGGCAC+AGG + chr8_4:53658356-53658375 None:intergenic 50.0%
!! CCTGTGCCGGTTCAAATTCA+TGG - chr8_4:53658353-53658372 Msa1333500:CDS 50.0%
GGCAATGGAGGAAAGAAAGG+TGG - chr8_4:53658392-53658411 Msa1333500:CDS 50.0%
CATGGCATGAATGGGGGAAA+TGG - chr8_4:53658443-53658462 Msa1333500:CDS 50.0%
GGAGGCAACAAAAATAGCGG+TGG - chr8_4:53658521-53658540 Msa1333500:intron 50.0%
AAATAGCGGTGGTTTACCGG+AGG - chr8_4:53658532-53658551 Msa1333500:intron 50.0%
!!! CCACCACCATTTTTAGCCTC+CGG + chr8_4:53658551-53658570 None:intergenic 50.0%
!! GAGGCTAAAAATGGTGGTGG+TGG - chr8_4:53658551-53658570 Msa1333500:intron 50.0%
!! GCTAAAAATGGTGGTGGTGG+TGG - chr8_4:53658554-53658573 Msa1333500:intron 50.0%
!! AAAAATGGTGGTGGTGGTGG+AGG - chr8_4:53658557-53658576 Msa1333500:intron 50.0%
!! GGTGGTGGAGGAAACAAGAA+TGG - chr8_4:53658569-53658588 Msa1333500:intron 50.0%
GGTGGAGGAAACAAGAATGG+AGG - chr8_4:53658572-53658591 Msa1333500:intron 50.0%
TACCTGCACCACTCATAGCA+GGG + chr8_4:53658736-53658755 None:intergenic 50.0%
TTACCTGCACCACTCATAGC+AGG + chr8_4:53658737-53658756 None:intergenic 50.0%
TTCTGCTGTTCAAGGCCTAC+CGG - chr8_4:53658805-53658824 Msa1333500:intron 50.0%
GGAGGATACTTTCAAGGTGG+TGG - chr8_4:53658833-53658852 Msa1333500:intron 50.0%
! GGATACTTTCAAGGTGGTGG+TGG - chr8_4:53658836-53658855 Msa1333500:intron 50.0%
TCCAAGCCCGGAAATGATGT+CGG - chr8_4:53658859-53658878 Msa1333500:intron 50.0%
GAACCAACAACGTGCTGCAA+TGG - chr8_4:53658955-53658974 Msa1333500:five_prime_UTR 50.0%
CTACCATTGCAGCACGTTGT+TGG + chr8_4:53658961-53658980 None:intergenic 50.0%
! GGTGGCCTTGCATACATCAT+TGG + chr8_4:53659007-53659026 None:intergenic 50.0%
GAGGAGGATAAGGGTAAGGA+GGG + chr8_4:53659051-53659070 None:intergenic 50.0%
TCTGGAGGAGGATAAGGGTA+AGG + chr8_4:53659055-53659074 None:intergenic 50.0%
CACTGAGATCTTACCGGCGA+CGG - chr8_4:53656864-53656883 Msa1333500:three_prime_UTR 55.0%
AGAGAGACCGAGAATGACGC+CGG + chr8_4:53656896-53656915 None:intergenic 55.0%
GGTCACGGTTTCAGGGAATG+TGG - chr8_4:53657650-53657669 Msa1333500:CDS 55.0%
!! TTCTGATTCGGGTTCTGGCC+CGG + chr8_4:53658038-53658057 None:intergenic 55.0%
! GGAGGAAAGAAAGGTGGTGG+TGG - chr8_4:53658398-53658417 Msa1333500:CDS 55.0%
!! GGAAAGAAAGGTGGTGGTGG+TGG - chr8_4:53658401-53658420 Msa1333500:CDS 55.0%
CCGGAGGCTAAAAATGGTGG+TGG - chr8_4:53658548-53658567 Msa1333500:intron 55.0%
!! CAAAAAATGATGGGCGGCGG+AGG - chr8_4:53658698-53658717 Msa1333500:CDS 55.0%
CATCCTCACCCTGCTATGAG+TGG - chr8_4:53658725-53658744 Msa1333500:CDS 55.0%
!! TGAAAGTATCCTCCGCCAGC+CGG + chr8_4:53658827-53658846 None:intergenic 55.0%
GCTGGCGGAGGATACTTTCA+AGG - chr8_4:53658827-53658846 Msa1333500:intron 55.0%
GGCGGAGGATACTTTCAAGG+TGG - chr8_4:53658830-53658849 Msa1333500:intron 55.0%
CCAAGCCCGGAAATGATGTC+GGG - chr8_4:53658860-53658879 Msa1333500:intron 55.0%
GGAGGAGGATAAGGGTAAGG+AGG + chr8_4:53659052-53659071 None:intergenic 55.0%
TGGCTGTAAGGATCTGGAGG+AGG + chr8_4:53659067-53659086 None:intergenic 55.0%
GCATGGCTGTAAGGATCTGG+AGG + chr8_4:53659070-53659089 None:intergenic 55.0%
CGTCGCCGGTAAGATCTCAG+TGG + chr8_4:53656866-53656885 None:intergenic 60.0%
TGAGATCTTACCGGCGACGG+TGG - chr8_4:53656867-53656886 Msa1333500:three_prime_UTR 60.0%
AGTTGCTGCTGCTGCTGCTG+AGG + chr8_4:53657921-53657940 None:intergenic 60.0%
!! TTCTGGCCCGGGTGCTGATT+CGG + chr8_4:53658026-53658045 None:intergenic 60.0%
!! TCTGATTCGGGTTCTGGCCC+GGG + chr8_4:53658037-53658056 None:intergenic 60.0%
!! AATGGCGGTGGTGGCGGAAA+CGG - chr8_4:53658308-53658327 Msa1333500:CDS 60.0%
GGAAACGGGCAGAAACCTGG+TGG - chr8_4:53658323-53658342 Msa1333500:CDS 60.0%
AACGGGCAGAAACCTGGTGG+TGG - chr8_4:53658326-53658345 Msa1333500:CDS 60.0%
! GGAGGAAAGAAAGGTGGCGG+CGG - chr8_4:53658470-53658489 Msa1333500:CDS 60.0%
!! AAAATGATGGGCGGCGGAGG+TGG - chr8_4:53658701-53658720 Msa1333500:CDS 60.0%
GCACCACTCATAGCAGGGTG+AGG + chr8_4:53658731-53658750 None:intergenic 60.0%
CACCCTGCTATGAGTGGTGC+AGG - chr8_4:53658731-53658750 Msa1333500:CDS 60.0%
CCCGACATCATTTCCGGGCT+TGG + chr8_4:53658863-53658882 None:intergenic 60.0%
GTGGCCACCGGCGTCATTCT+CGG - chr8_4:53656886-53656905 Msa1333500:three_prime_UTR 65.0%
GAGACCGAGAATGACGCCGG+TGG + chr8_4:53656893-53656912 None:intergenic 65.0%
GATGCCGCCGAATCAGCACC+CGG - chr8_4:53658016-53658035 Msa1333500:CDS 65.0%
ATGCCGCCGAATCAGCACCC+GGG - chr8_4:53658017-53658036 Msa1333500:CDS 65.0%
!! ATGGCGGTGGTGGCGGAAAC+GGG - chr8_4:53658309-53658328 Msa1333500:CDS 65.0%
GGCGGAAACGGGCAGAAACC+TGG - chr8_4:53658320-53658339 Msa1333500:CDS 65.0%
!! GGGCAGAAACCTGGTGGTGG+AGG - chr8_4:53658329-53658348 Msa1333500:CDS 65.0%
!! TGGTGGTGGAGGACCTGTGC+CGG - chr8_4:53658340-53658359 Msa1333500:CDS 65.0%
! GGAAAGAAAGGTGGCGGCGG+TGG - chr8_4:53658473-53658492 Msa1333500:CDS 65.0%
! AAGAAAGGTGGCGGCGGTGG+TGG - chr8_4:53658476-53658495 Msa1333500:CDS 65.0%
GCTGTTCAAGGCCTACCGGC+TGG - chr8_4:53658809-53658828 Msa1333500:intron 65.0%
! GTTCAAGGCCTACCGGCTGG+CGG - chr8_4:53658812-53658831 Msa1333500:intron 65.0%
AGTATCCTCCGCCAGCCGGT+AGG + chr8_4:53658823-53658842 None:intergenic 65.0%
!! AGGTGGTGGTGGTCCAAGCC+CGG - chr8_4:53658847-53658866 Msa1333500:intron 65.0%
TTACCGGCGACGGTGGCCAC+CGG - chr8_4:53656874-53656893 Msa1333500:three_prime_UTR 70.0%
!! TGGCCCGGGTGCTGATTCGG+CGG + chr8_4:53658023-53658042 None:intergenic 70.0%
GGCACAGGTCCTCCACCACC+AGG + chr8_4:53658341-53658360 None:intergenic 70.0%
! AAAGGTGGCGGCGGTGGTGG+AGG - chr8_4:53658479-53658498 Msa1333500:CDS 70.0%
! CAAGGCCTACCGGCTGGCGG+AGG - chr8_4:53658815-53658834 Msa1333500:intron 75.0%
! ACGCCGGTGGCCACCGTCGC+CGG + chr8_4:53656880-53656899 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 53656773 53659378 53656773 ID=Msa1333500;Name=Msa1333500
chr8_4 mRNA 53656773 53659378 53656773 ID=Msa1333500-mRNA-1;Parent=Msa1333500;Name=Msa1333500-mRNA-1;_AED=0.06;_eAED=0.06;_QI=457|1|1|1|1|1|3|249|540
chr8_4 exon 53656773 53658538 53656773 ID=Msa1333500-mRNA-1:exon:16487;Parent=Msa1333500-mRNA-1
chr8_4 exon 53658680 53658755 53658680 ID=Msa1333500-mRNA-1:exon:16486;Parent=Msa1333500-mRNA-1
chr8_4 exon 53658892 53659378 53658892 ID=Msa1333500-mRNA-1:exon:16485;Parent=Msa1333500-mRNA-1
chr8_4 five_prime_UTR 53658922 53659378 53658922 ID=Msa1333500-mRNA-1:five_prime_utr;Parent=Msa1333500-mRNA-1
chr8_4 CDS 53658892 53658921 53658892 ID=Msa1333500-mRNA-1:cds;Parent=Msa1333500-mRNA-1
chr8_4 CDS 53658680 53658755 53658680 ID=Msa1333500-mRNA-1:cds;Parent=Msa1333500-mRNA-1
chr8_4 CDS 53657022 53658538 53657022 ID=Msa1333500-mRNA-1:cds;Parent=Msa1333500-mRNA-1
chr8_4 three_prime_UTR 53656773 53657021 53656773 ID=Msa1333500-mRNA-1:three_prime_utr;Parent=Msa1333500-mRNA-1
Gene Sequence

>Msa1333500

ATGAGTAAAGAAGAATTTTTGAAGATACAGAAATCTGTTCTAAAAGTGAACATACACTGTGATGGATGTAAACATAAAGTAAAGAAAATCTTACAGAAAATTGATGGAGTATTTACAACTGAGATAGATGCAGAACAAGGAAAGGTCACGGTTTCAGGGAATGTGGACCCAAATGTTCTCATCAAGAAGCTTGCAAAATCAGGGAAACATGCTGAGTTATGGAGTGTTCCCAAACCAAACAATAATCAAAATAATAACCAGAACAACGTTGTTAATCAGTTAAAGAACATGCAAATTGACAATGGCAAAGGTGGTGGAAACAACAAAGGTCAGAATCAAAACCAGAAGGGTGGTGGAAACAATAACCAACCCAAAGGTGGTCAACAAATACAACAACTACAAGGACTAAATCCTCAGCAGCAGCAGCAGCAACTTCAGCTTCAACAACAGTTGCAACAACTTCAACAGATGAAAGGGTTTCAAGATCTAGCTCAATTTAAAGGCATGAAGATGCCGCCGAATCAGCACCCGGGCCAGAACCCGAATCAGAATCCTAAAGATGCTAAAGGTGTTAAATTTGATGTGCCTGAAGATGAGGATGATTTTAGTGATGATGAAATGGATGATTTTGATGACGACGAAGATGATGATGAAGATTTTGATGATGAGTTTGATGATGAAATGGTTGGTTTGCCACCTAATAAGATGAAACCCCCTTCTATGCCTATGGGGAATGGTGCTCACATGATGTTGAATGGGAATCATCCACAGCTTATGAGTGCTATGAAGGGTGGCAATTTTAATGGCGGTGGTGGCGGAAACGGGCAGAAACCTGGTGGTGGAGGACCTGTGCCGGTTCAAATTCATGGCATGAATGGTGGAAATGGCAATGGAGGAAAGAAAGGTGGTGGTGGTGGACCTGTGCCAGTTCAAATTCATGGCATGAATGGGGGAAATGGTAATGGAGGAAAGAAAGGTGGCGGCGGTGGTGGAGGAAATAATCAGAATCACGGTGGAGGCAACAAAAATAGCGGTGGTTTACCGGAGGCTAAAAATGGTGGTGGTGGTGGAGGAAACAAGAATGGAGGAAATCATAATAGCAATAATCAAAATAACAATGGGGGTAAAAAGGGTATGTCGATACCAATTGCTGGTAGTGGTGGTAATGTTCAAGCTATGAACAATGGATTTCAAAAAATGATGGGCGGCGGAGGTGGTCATCCTCACCCTGCTATGAGTGGTGCAGGTAATAATGTGGGACCAATGAGTATGCGAATGAACCAAATGGGTGGTAACATTTCTGCTGTTCAAGGCCTACCGGCTGGCGGAGGATACTTTCAAGGTGGTGGTGGTCCAAGCCCGGAAATGATGTCGGGTAATCCTTATCAACAACAGCAACATCAGCAGCAGCAACAACAACAGCAACAGTTAATGGCTGCTGCAATGATGAACCAACAACGTGCTGCAATGGTAGAAAATGATCAAAGATTTCAGCAGCCAATGATGTATGCAAGGCCACCTCCTGCTGTGAATTACATGTACCCTCCTTACCCTTATCCTCCTCCAGATCCTTACAGCCATGCTTTCAGTGATGAAAACACTTCCAGCTGCAATGTCATGTGA
Protein sequence

>Msa1333500

MSKEEFLKIQKSVLKVNIHCDGCKHKVKKILQKIDGVFTTEIDAEQGKVTVSGNVDPNVLIKKLAKSGKHAELWSVPKPNNNQNNNQNNVVNQLKNMQIDNGKGGGNNKGQNQNQKGGGNNNQPKGGQQIQQLQGLNPQQQQQQLQLQQQLQQLQQMKGFQDLAQFKGMKMPPNQHPGQNPNQNPKDAKGVKFDVPEDEDDFSDDEMDDFDDDEDDDEDFDDEFDDEMVGLPPNKMKPPSMPMGNGAHMMLNGNHPQLMSAMKGGNFNGGGGGNGQKPGGGGPVPVQIHGMNGGNGNGGKKGGGGGPVPVQIHGMNGGNGNGGKKGGGGGGGNNQNHGGGNKNSGGLPEAKNGGGGGGNKNGGNHNSNNQNNNGGKKGMSIPIAGSGGNVQAMNNGFQKMMGGGGGHPHPAMSGAGNNVGPMSMRMNQMGGNISAVQGLPAGGGYFQGGGGPSPEMMSGNPYQQQQHQQQQQQQQQLMAAAMMNQQRAAMVENDQRFQQPMMYARPPPAVNYMYPPYPYPPPDPYSHAFSDENTSSCNVM*