AlfalfaGEDB | Msa1403380

M. sativa cultivar zhongmu-4 / Msa1403380

KEGG: K09874
GO: GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006855 GO:0007154 GO:0008150 GO:0008509 GO:0008643 GO:0009605 GO:0009987 GO:0009991 GO:0010036 GO:0015075 GO:0015103 GO:0015144 GO:0015166 GO:0015168 GO:0015204 GO:0015238 GO:0015318 GO:0015698 GO:0015791 GO:0015793 GO:0015840 GO:0015850 GO:0015893 GO:0016020 GO:0016021 GO:0019755 GO:0022804 GO:0022857 GO:0031224 GO:0031226 GO:0031667 GO:0031668 GO:0031669 GO:0034219 GO:0034220 GO:0035445 GO:0042221 GO:0042493 GO:0042886 GO:0042887 GO:0044425 GO:0044459 GO:0044464 GO:0046713 GO:0046715 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0070887 GO:0071496 GO:0071702 GO:0071705 GO:0071918 GO:0071944 GO:0080029 GO:0098656 GO:0098660 GO:0098661 GO:1901618
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403380	KEH28543.1	100.000	89	0	0	3	91	130	218	4.47e-54	177

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403380	sp\|Q9SAI4\|NIP61_ARATH	76.404	89	16	1	3	91	222	305	1.30e-36	128

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403380	A0A072UHE5	100.000	89	0	0	3	91	130	218	2.14e-54	177

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0739410	Msa1403380	0.814562	1.446105e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403380	MtrunA17_Chr4g0001121	98.876	89	1	0	3	91	10	98	4.70e-58	173
Msa1403380	MtrunA17_Chr4g0001221	100.000	89	0	0	3	91	217	305	2.43e-56	175
Msa1403380	MtrunA17_Chr2g0333771	83.146	89	15	0	3	91	222	310	5.72e-45	146
Msa1403380	MtrunA17_Chr1g0201331	73.611	72	19	0	3	74	216	287	1.64e-31	111
Msa1403380	MtrunA17_Chr8g0379901	56.098	82	36	0	3	84	182	263	5.27e-29	104
Msa1403380	MtrunA17_Chr2g0284611	56.098	82	36	0	3	84	181	262	1.11e-28	103
Msa1403380	MtrunA17_Chr2g0284591	56.098	82	36	0	3	84	179	260	2.37e-28	102
Msa1403380	MtrunA17_Chr8g0379911	54.878	82	37	0	3	84	181	262	5.76e-28	102
Msa1403380	MtrunA17_Chr2g0284601	51.685	89	39	1	3	91	133	217	3.01e-26	96.3
Msa1403380	MtrunA17_Chr2g0284581	51.685	89	39	1	3	91	181	265	4.51e-26	97.1
Msa1403380	MtrunA17_Chr4g0044201	47.191	89	47	0	3	91	167	255	1.53e-25	95.5
Msa1403380	MtrunA17_Chr2g0284631	60.294	68	27	0	3	70	234	301	6.30e-25	95.1
Msa1403380	MtrunA17_Chr7g0217331	49.425	87	42	1	4	90	180	264	9.42e-24	90.9
Msa1403380	MtrunA17_Chr5g0435051	48.235	85	37	1	3	80	182	266	2.51e-23	89.7
Msa1403380	MtrunA17_Chr5g0426131	55.224	67	30	0	5	71	183	249	1.52e-21	85.1
Msa1403380	MtrunA17_Chr3g0079421	54.412	68	31	0	3	70	168	235	1.58e-20	82.4
Msa1403380	MtrunA17_Chr1g0184541	44.737	76	42	0	3	78	204	279	5.64e-17	73.2
Msa1403380	MtrunA17_Chr2g0331261	42.667	75	41	2	2	75	169	242	2.41e-12	60.1
Msa1403380	MtrunA17_Chr5g0399341	45.614	57	31	0	2	58	167	223	1.06e-11	58.5
Msa1403380	MtrunA17_Chr8g0340161	45.614	57	31	0	2	58	168	224	3.35e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403380	AT1G80760.1	76.404	89	16	1	3	91	222	305	1.32e-37	128
Msa1403380	AT4G10380.1	65.556	90	25	2	3	91	220	304	9.28e-32	112
Msa1403380	AT4G18910.1	48.864	88	45	0	3	90	202	289	2.25e-25	95.9
Msa1403380	AT4G19030.1	51.136	88	43	0	3	90	205	292	2.75e-25	95.9
Msa1403380	AT5G37810.1	48.780	82	42	0	3	84	186	267	2.96e-24	92.8
Msa1403380	AT5G37820.1	49.383	81	41	0	4	84	187	267	8.31e-24	91.7
Msa1403380	AT5G37820.2	49.383	81	41	0	4	84	223	303	9.84e-24	92.0
Msa1403380	AT1G31885.2	43.678	87	49	0	4	90	140	226	2.65e-21	84.7
Msa1403380	AT1G31885.1	43.678	87	49	0	4	90	194	280	4.19e-21	85.1
Msa1403380	AT2G34390.2	48.276	87	42	2	5	91	157	240	1.31e-19	80.1
Msa1403380	AT2G34390.1	48.276	87	42	2	5	91	199	282	2.53e-19	79.7
Msa1403380	AT2G29870.1	45.977	87	44	1	5	91	50	133	8.17e-19	75.5
Msa1403380	AT3G06100.3	41.429	70	41	0	2	71	107	176	1.31e-16	71.2
Msa1403380	AT3G06100.1	41.429	70	41	0	2	71	188	257	5.87e-16	70.9
Msa1403380	AT3G06100.2	39.726	73	44	0	2	74	140	212	6.44e-16	70.1
Msa1403380	AT3G16240.1	47.368	57	30	0	2	58	168	224	1.99e-12	60.8
Msa1403380	AT4G17340.1	45.614	57	31	0	2	58	168	224	9.89e-12	58.9
Msa1403380	AT5G47450.1	42.857	77	36	2	2	70	168	244	5.09e-11	57.0
Msa1403380	AT1G73190.1	34.118	85	56	0	2	86	178	262	5.40e-11	57.0

Find 49 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCATGTGGCCTCTGGGTATT+TGG	0.110117	tig0022956:+3177	Msa1403380:three_prime_UTR
TCTGGGTATTTGGGTTTAAT+AGG	0.249558	tig0022956:+3187	Msa1403380:three_prime_UTR
GCTGGTCCTAGTGTTCTTAC+TGG	0.259920	tig0022956:-2686	None:intergenic
GCCCCTATTCTTGGTGCTCT+TGG	0.301422	tig0022956:+2752	Msa1403380:CDS
TTCATTGAAGCTCCAGTTGC+AGG	0.320017	tig0022956:-2662	None:intergenic
CATGTGGCCTCTGGGTATTT+GGG	0.321412	tig0022956:+3178	Msa1403380:three_prime_UTR
GTTCTCTAGCATGTGGCCTC+TGG	0.323008	tig0022956:+3169	Msa1403380:three_prime_UTR
CATCATCTTCTGAAACTTCC+AGG	0.332213	tig0022956:-2848	None:intergenic
CTTGGTGCTCTTGGTGGGGC+AGG	0.355406	tig0022956:+2761	Msa1403380:CDS
TATCTCATAGCCCCTATTCT+TGG	0.361100	tig0022956:+2743	Msa1403380:CDS
CACCAAGAGCACCAAGAATA+GGG	0.367072	tig0022956:-2754	None:intergenic
ATATACTGCAGGCCTGCAAC+TGG	0.394672	tig0022956:+2650	Msa1403380:intron
TAGTTGTTTGCAGCAATTGC+TGG	0.400189	tig0022956:-2704	None:intergenic
ATGCTCAACATACTCATTGC+TGG	0.401599	tig0022956:+2056	Msa1403380:CDS
CCTATTCTTGGTGCTCTTGG+TGG	0.402986	tig0022956:+2755	Msa1403380:CDS
CCACCAAGAGCACCAAGAAT+AGG	0.407883	tig0022956:-2755	None:intergenic
CTATTCTTGGTGCTCTTGGT+GGG	0.417084	tig0022956:+2756	Msa1403380:CDS
ATACAAAGATTCTAATTAAG+AGG	0.432938	tig0022956:-2911	None:intergenic
ATGCAGGTGGGAGAACTTGC+GGG	0.438650	tig0022956:+2011	Msa1403380:CDS
TTCTCTAGCATGTGGCCTCT+GGG	0.447929	tig0022956:+3170	Msa1403380:three_prime_UTR
CTGATTTATGTTTATCAAAG+TGG	0.466449	tig0022956:-3040	None:intergenic
TCAGAGTTAAACTCTTCATC+AGG	0.475612	tig0022956:-2806	None:intergenic
GTGTGCATATGTAGCTAGCT+AGG	0.482837	tig0022956:-3002	None:intergenic
CAAACAACTACAAAGGCATA+TGG	0.493499	tig0022956:+2717	Msa1403380:CDS
TATTCTTGGTGCTCTTGGTG+GGG	0.515515	tig0022956:+2757	Msa1403380:CDS
ATCATCTTCTGAAACTTCCA+GGG	0.521569	tig0022956:-2847	None:intergenic
GAAACTTCCAGGGGCAGAAG+AGG	0.526255	tig0022956:-2837	None:intergenic
GTTAAAGCCTCTTCTGCCCC+TGG	0.527500	tig0022956:+2830	Msa1403380:CDS
TGCTCAACATACTCATTGCT+GGG	0.532589	tig0022956:+2057	Msa1403380:CDS
AACACAAAATTATATTCTCA+TGG	0.549209	tig0022956:-3228	None:intergenic
GAACTTGCGGGAATCGCGGT+GGG	0.555721	tig0022956:+2023	Msa1403380:CDS
GGGAGAACTTGCGGGAATCG+CGG	0.575910	tig0022956:+2019	Msa1403380:CDS
AGAACTTGCGGGAATCGCGG+TGG	0.586192	tig0022956:+2022	Msa1403380:CDS
ATGAATCCAGTAAGAACACT+AGG	0.586393	tig0022956:+2680	Msa1403380:CDS
AATGCAGGTGGGAGAACTTG+CGG	0.593498	tig0022956:+2010	Msa1403380:exon
ACCAAGAGCACCAAGAATAG+GGG	0.596229	tig0022956:-2753	None:intergenic
TGTGCATATGTAGCTAGCTA+GGG	0.601836	tig0022956:-3001	None:intergenic
GTGGTAACATGATGCAATGC+AGG	0.602684	tig0022956:+1995	Msa1403380:exon
GACATTAGTTCTCTAGCATG+TGG	0.604049	tig0022956:+3162	Msa1403380:three_prime_UTR
TGATGAAGAGTTTAACTCTG+AGG	0.607367	tig0022956:+2808	Msa1403380:CDS
TTAATGTAGATAATGACTTG+TGG	0.635246	tig0022956:+1976	Msa1403380:five_prime_UTR
CTATCTCTAGTAATAAACAA+TGG	0.645773	tig0022956:-3258	None:intergenic
TCATCTTCTGAAACTTCCAG+GGG	0.658967	tig0022956:-2846	None:intergenic
ATTGCTGCAAACAACTACAA+AGG	0.670460	tig0022956:+2710	Msa1403380:CDS
GTAACATGATGCAATGCAGG+TGG	0.696993	tig0022956:+1998	Msa1403380:exon
TAACATGATGCAATGCAGGT+GGG	0.710115	tig0022956:+1999	Msa1403380:exon
AAGATGATGAATCATTCACA+AGG	0.712240	tig0022956:+2862	Msa1403380:exon
GAGTATGTTGAGCATGACAG+TGG	0.737398	tig0022956:-2048	None:intergenic
TATTAAACCCAAATACCCAG+AGG	0.772671	tig0022956:-3185	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATAAAAATATATATTTAA+TGG	-	tig0022956:2195-2214	None:intergenic	0.0%
!!	TAATAAAAATATATATTTAA+TGG	-	tig0022956:2195-2214	None:intergenic	0.0%
!!	TTGTCAAAAAAACAATAATA+TGG	-	tig0022956:1936-1955	None:intergenic	15.0%
!!!	TATTATTGTTTTTTTGACAA+AGG	+	tig0022956:1936-1955	Msa1403380:five_prime_UTR	15.0%
!!	TTGTCAAAAAAACAATAATA+TGG	-	tig0022956:1936-1955	None:intergenic	15.0%
!!!	TATTATTGTTTTTTTGACAA+AGG	+	tig0022956:1936-1955	Msa1403380:five_prime_UTR	15.0%
!!!	TTTATATCATTTGTTTTGTC+GGG	-	tig0022956:2379-2398	None:intergenic	20.0%
!!!	GTTTATATCATTTGTTTTGT+CGG	-	tig0022956:2380-2399	None:intergenic	20.0%
!!	ATACAAAGATTCTAATTAAG+AGG	-	tig0022956:2914-2933	None:intergenic	20.0%
!!	AACACAAAATTATATTCTCA+TGG	-	tig0022956:3231-3250	None:intergenic	20.0%
!!!	AATATAATTTTGTGTTAGTG+AGG	+	tig0022956:3235-3254	Msa1403380:three_prime_UTR	20.0%
!!!	TTTATATCATTTGTTTTGTC+GGG	-	tig0022956:2379-2398	None:intergenic	20.0%
!!!	GTTTATATCATTTGTTTTGT+CGG	-	tig0022956:2380-2399	None:intergenic	20.0%
!!	ATACAAAGATTCTAATTAAG+AGG	-	tig0022956:2914-2933	None:intergenic	20.0%
!!	AACACAAAATTATATTCTCA+TGG	-	tig0022956:3231-3250	None:intergenic	20.0%
!!!	AATATAATTTTGTGTTAGTG+AGG	+	tig0022956:3235-3254	Msa1403380:three_prime_UTR	20.0%
!	TTAATGTAGATAATGACTTG+TGG	+	tig0022956:1976-1995	Msa1403380:five_prime_UTR	25.0%
!	CGAATGTGATTAAAATAATG+AGG	-	tig0022956:2101-2120	None:intergenic	25.0%
!	TTTAACTCTTAAAGCAAATG+AGG	-	tig0022956:2311-2330	None:intergenic	25.0%
!	CTAGACACTAAAATATTTAG+TGG	+	tig0022956:2334-2353	Msa1403380:intron	25.0%
!	AAAGATCCAATATTCCATTT+AGG	+	tig0022956:2415-2434	Msa1403380:intron	25.0%
!	GAAATTCAGACATGTAAAAA+AGG	+	tig0022956:2522-2541	Msa1403380:intron	25.0%
!	AAATACATACACGTAAAAAC+AGG	-	tig0022956:2556-2575	None:intergenic	25.0%
!	CTGATTTATGTTTATCAAAG+TGG	-	tig0022956:3043-3062	None:intergenic	25.0%
!!!	AAAACAGCACTGATTTTTTT+TGG	-	tig0022956:3118-3137	None:intergenic	25.0%
!	CTATCTCTAGTAATAAACAA+TGG	-	tig0022956:3261-3280	None:intergenic	25.0%
!	TTAATGTAGATAATGACTTG+TGG	+	tig0022956:1976-1995	Msa1403380:five_prime_UTR	25.0%
!	CGAATGTGATTAAAATAATG+AGG	-	tig0022956:2101-2120	None:intergenic	25.0%
!	TTTAACTCTTAAAGCAAATG+AGG	-	tig0022956:2311-2330	None:intergenic	25.0%
!	CTAGACACTAAAATATTTAG+TGG	+	tig0022956:2334-2353	Msa1403380:intron	25.0%
!	AAAGATCCAATATTCCATTT+AGG	+	tig0022956:2415-2434	Msa1403380:intron	25.0%
!	GAAATTCAGACATGTAAAAA+AGG	+	tig0022956:2522-2541	Msa1403380:intron	25.0%
!	AAATACATACACGTAAAAAC+AGG	-	tig0022956:2556-2575	None:intergenic	25.0%
!	CTGATTTATGTTTATCAAAG+TGG	-	tig0022956:3043-3062	None:intergenic	25.0%
!!!	AAAACAGCACTGATTTTTTT+TGG	-	tig0022956:3118-3137	None:intergenic	25.0%
!	CTATCTCTAGTAATAAACAA+TGG	-	tig0022956:3261-3280	None:intergenic	25.0%
	AAATAATGAGGAGTGAAAGT+TGG	-	tig0022956:2089-2108	None:intergenic	30.0%
	AGATACGTTGCAAATTCTTA+AGG	+	tig0022956:2263-2282	Msa1403380:intron	30.0%
!	TTTTCTTATCTCACACTTCT+CGG	+	tig0022956:2476-2495	Msa1403380:intron	30.0%
	TTTGACACTAAATATACTGC+AGG	+	tig0022956:2639-2658	Msa1403380:intron	30.0%
	AAGATGATGAATCATTCACA+AGG	+	tig0022956:2862-2881	Msa1403380:exon	30.0%
	AAATAATGAGGAGTGAAAGT+TGG	-	tig0022956:2089-2108	None:intergenic	30.0%
	AGATACGTTGCAAATTCTTA+AGG	+	tig0022956:2263-2282	Msa1403380:intron	30.0%
!	TTTTCTTATCTCACACTTCT+CGG	+	tig0022956:2476-2495	Msa1403380:intron	30.0%
	TTTGACACTAAATATACTGC+AGG	+	tig0022956:2639-2658	Msa1403380:intron	30.0%
	AAGATGATGAATCATTCACA+AGG	+	tig0022956:2862-2881	Msa1403380:exon	30.0%
	CGTTGCAAATTCTTAAGGTA+TGG	+	tig0022956:2268-2287	Msa1403380:intron	35.0%
	ACAAGGCCTAAATGGAATAT+TGG	-	tig0022956:2424-2443	None:intergenic	35.0%
	TAAGATCCTGTTAGGAAACA+AGG	-	tig0022956:2441-2460	None:intergenic	35.0%
	GCAAACAATAAGATCCTGTT+AGG	-	tig0022956:2449-2468	None:intergenic	35.0%
	ATGAATCCAGTAAGAACACT+AGG	+	tig0022956:2680-2699	Msa1403380:CDS	35.0%
	ATTGCTGCAAACAACTACAA+AGG	+	tig0022956:2710-2729	Msa1403380:CDS	35.0%
!	CAAACAACTACAAAGGCATA+TGG	+	tig0022956:2717-2736	Msa1403380:CDS	35.0%
	TCAGAGTTAAACTCTTCATC+AGG	-	tig0022956:2809-2828	None:intergenic	35.0%
	TGATGAAGAGTTTAACTCTG+AGG	+	tig0022956:2808-2827	Msa1403380:CDS	35.0%
!!	ATCATCTTCTGAAACTTCCA+GGG	-	tig0022956:2850-2869	None:intergenic	35.0%
	TATTAAACCCAAATACCCAG+AGG	-	tig0022956:3188-3207	None:intergenic	35.0%
!	TCTGGGTATTTGGGTTTAAT+AGG	+	tig0022956:3187-3206	Msa1403380:three_prime_UTR	35.0%
	CGTTGCAAATTCTTAAGGTA+TGG	+	tig0022956:2268-2287	Msa1403380:intron	35.0%
	ACAAGGCCTAAATGGAATAT+TGG	-	tig0022956:2424-2443	None:intergenic	35.0%
	TAAGATCCTGTTAGGAAACA+AGG	-	tig0022956:2441-2460	None:intergenic	35.0%
	GCAAACAATAAGATCCTGTT+AGG	-	tig0022956:2449-2468	None:intergenic	35.0%
	ATGAATCCAGTAAGAACACT+AGG	+	tig0022956:2680-2699	Msa1403380:CDS	35.0%
	ATTGCTGCAAACAACTACAA+AGG	+	tig0022956:2710-2729	Msa1403380:CDS	35.0%
!	CAAACAACTACAAAGGCATA+TGG	+	tig0022956:2717-2736	Msa1403380:CDS	35.0%
	TCAGAGTTAAACTCTTCATC+AGG	-	tig0022956:2809-2828	None:intergenic	35.0%
	TGATGAAGAGTTTAACTCTG+AGG	+	tig0022956:2808-2827	Msa1403380:CDS	35.0%
!!	ATCATCTTCTGAAACTTCCA+GGG	-	tig0022956:2850-2869	None:intergenic	35.0%
	TATTAAACCCAAATACCCAG+AGG	-	tig0022956:3188-3207	None:intergenic	35.0%
!	TCTGGGTATTTGGGTTTAAT+AGG	+	tig0022956:3187-3206	Msa1403380:three_prime_UTR	35.0%
	TAACATGATGCAATGCAGGT+GGG	+	tig0022956:1999-2018	Msa1403380:exon	40.0%
	ATGCTCAACATACTCATTGC+TGG	+	tig0022956:2056-2075	Msa1403380:CDS	40.0%
	TGCTCAACATACTCATTGCT+GGG	+	tig0022956:2057-2076	Msa1403380:CDS	40.0%
	GTTAGGAAACAAGGCCTAAA+TGG	-	tig0022956:2432-2451	None:intergenic	40.0%
!!	TTTAGGCCTTGTTTCCTAAC+AGG	+	tig0022956:2432-2451	Msa1403380:intron	40.0%
!	CTCACACTTCTCGGATATTT+TGG	+	tig0022956:2485-2504	Msa1403380:intron	40.0%
!!	TCGGAAGTTTCTGGTTTGTT+CGG	-	tig0022956:2585-2604	None:intergenic	40.0%
	TAGTTGTTTGCAGCAATTGC+TGG	-	tig0022956:2707-2726	None:intergenic	40.0%
	TATCTCATAGCCCCTATTCT+TGG	+	tig0022956:2743-2762	Msa1403380:CDS	40.0%
!!	TCATCTTCTGAAACTTCCAG+GGG	-	tig0022956:2849-2868	None:intergenic	40.0%
!!	CATCATCTTCTGAAACTTCC+AGG	-	tig0022956:2851-2870	None:intergenic	40.0%
	TGTGCATATGTAGCTAGCTA+GGG	-	tig0022956:3004-3023	None:intergenic	40.0%
!	GACATTAGTTCTCTAGCATG+TGG	+	tig0022956:3162-3181	Msa1403380:three_prime_UTR	40.0%
	TAACATGATGCAATGCAGGT+GGG	+	tig0022956:1999-2018	Msa1403380:exon	40.0%
	ATGCTCAACATACTCATTGC+TGG	+	tig0022956:2056-2075	Msa1403380:CDS	40.0%
	TGCTCAACATACTCATTGCT+GGG	+	tig0022956:2057-2076	Msa1403380:CDS	40.0%
	GTTAGGAAACAAGGCCTAAA+TGG	-	tig0022956:2432-2451	None:intergenic	40.0%
!!	TTTAGGCCTTGTTTCCTAAC+AGG	+	tig0022956:2432-2451	Msa1403380:intron	40.0%
!	CTCACACTTCTCGGATATTT+TGG	+	tig0022956:2485-2504	Msa1403380:intron	40.0%
!!	TCGGAAGTTTCTGGTTTGTT+CGG	-	tig0022956:2585-2604	None:intergenic	40.0%
	TAGTTGTTTGCAGCAATTGC+TGG	-	tig0022956:2707-2726	None:intergenic	40.0%
	TATCTCATAGCCCCTATTCT+TGG	+	tig0022956:2743-2762	Msa1403380:CDS	40.0%
!!	TCATCTTCTGAAACTTCCAG+GGG	-	tig0022956:2849-2868	None:intergenic	40.0%
!!	CATCATCTTCTGAAACTTCC+AGG	-	tig0022956:2851-2870	None:intergenic	40.0%
	TGTGCATATGTAGCTAGCTA+GGG	-	tig0022956:3004-3023	None:intergenic	40.0%
!	GACATTAGTTCTCTAGCATG+TGG	+	tig0022956:3162-3181	Msa1403380:three_prime_UTR	40.0%
	GTGGTAACATGATGCAATGC+AGG	+	tig0022956:1995-2014	Msa1403380:exon	45.0%
	GTAACATGATGCAATGCAGG+TGG	+	tig0022956:1998-2017	Msa1403380:exon	45.0%
	GAGTATGTTGAGCATGACAG+TGG	-	tig0022956:2051-2070	None:intergenic	45.0%
!	CTTTTCCACTCCACACTTCT+CGG	-	tig0022956:2604-2623	None:intergenic	45.0%
	TTCATTGAAGCTCCAGTTGC+AGG	-	tig0022956:2665-2684	None:intergenic	45.0%
!!	ACCAAGAGCACCAAGAATAG+GGG	-	tig0022956:2756-2775	None:intergenic	45.0%
!!	CACCAAGAGCACCAAGAATA+GGG	-	tig0022956:2757-2776	None:intergenic	45.0%
!!	CTATTCTTGGTGCTCTTGGT+GGG	+	tig0022956:2756-2775	Msa1403380:CDS	45.0%
!!	TATTCTTGGTGCTCTTGGTG+GGG	+	tig0022956:2757-2776	Msa1403380:CDS	45.0%
	GTGTGCATATGTAGCTAGCT+AGG	-	tig0022956:3005-3024	None:intergenic	45.0%
	GTGGTAACATGATGCAATGC+AGG	+	tig0022956:1995-2014	Msa1403380:exon	45.0%
	GTAACATGATGCAATGCAGG+TGG	+	tig0022956:1998-2017	Msa1403380:exon	45.0%
	GAGTATGTTGAGCATGACAG+TGG	-	tig0022956:2051-2070	None:intergenic	45.0%
!	CTTTTCCACTCCACACTTCT+CGG	-	tig0022956:2604-2623	None:intergenic	45.0%
	TTCATTGAAGCTCCAGTTGC+AGG	-	tig0022956:2665-2684	None:intergenic	45.0%
!!	ACCAAGAGCACCAAGAATAG+GGG	-	tig0022956:2756-2775	None:intergenic	45.0%
!!	CACCAAGAGCACCAAGAATA+GGG	-	tig0022956:2757-2776	None:intergenic	45.0%
!!	CTATTCTTGGTGCTCTTGGT+GGG	+	tig0022956:2756-2775	Msa1403380:CDS	45.0%
!!	TATTCTTGGTGCTCTTGGTG+GGG	+	tig0022956:2757-2776	Msa1403380:CDS	45.0%
	GTGTGCATATGTAGCTAGCT+AGG	-	tig0022956:3005-3024	None:intergenic	45.0%
	AATGCAGGTGGGAGAACTTG+CGG	+	tig0022956:2010-2029	Msa1403380:exon	50.0%
	CCACACTTCTCGGAAGTTTC+TGG	-	tig0022956:2594-2613	None:intergenic	50.0%
	CCAGAAACTTCCGAGAAGTG+TGG	+	tig0022956:2591-2610	Msa1403380:intron	50.0%
!	AACTTCCGAGAAGTGTGGAG+TGG	+	tig0022956:2596-2615	Msa1403380:intron	50.0%
	ATATACTGCAGGCCTGCAAC+TGG	+	tig0022956:2650-2669	Msa1403380:intron	50.0%
	GCTGGTCCTAGTGTTCTTAC+TGG	-	tig0022956:2689-2708	None:intergenic	50.0%
!!	CCACCAAGAGCACCAAGAAT+AGG	-	tig0022956:2758-2777	None:intergenic	50.0%
!!	CCTATTCTTGGTGCTCTTGG+TGG	+	tig0022956:2755-2774	Msa1403380:CDS	50.0%
	TTCTCTAGCATGTGGCCTCT+GGG	+	tig0022956:3170-3189	Msa1403380:three_prime_UTR	50.0%
	CATGTGGCCTCTGGGTATTT+GGG	+	tig0022956:3178-3197	Msa1403380:three_prime_UTR	50.0%
	AATGCAGGTGGGAGAACTTG+CGG	+	tig0022956:2010-2029	Msa1403380:exon	50.0%
	CCACACTTCTCGGAAGTTTC+TGG	-	tig0022956:2594-2613	None:intergenic	50.0%
	CCAGAAACTTCCGAGAAGTG+TGG	+	tig0022956:2591-2610	Msa1403380:intron	50.0%
!	AACTTCCGAGAAGTGTGGAG+TGG	+	tig0022956:2596-2615	Msa1403380:intron	50.0%
	ATATACTGCAGGCCTGCAAC+TGG	+	tig0022956:2650-2669	Msa1403380:intron	50.0%
	GCTGGTCCTAGTGTTCTTAC+TGG	-	tig0022956:2689-2708	None:intergenic	50.0%
!!	CCACCAAGAGCACCAAGAAT+AGG	-	tig0022956:2758-2777	None:intergenic	50.0%
!!	CCTATTCTTGGTGCTCTTGG+TGG	+	tig0022956:2755-2774	Msa1403380:CDS	50.0%
	TTCTCTAGCATGTGGCCTCT+GGG	+	tig0022956:3170-3189	Msa1403380:three_prime_UTR	50.0%
	CATGTGGCCTCTGGGTATTT+GGG	+	tig0022956:3178-3197	Msa1403380:three_prime_UTR	50.0%
	ATGCAGGTGGGAGAACTTGC+GGG	+	tig0022956:2011-2030	Msa1403380:CDS	55.0%
!!	GCCCCTATTCTTGGTGCTCT+TGG	+	tig0022956:2752-2771	Msa1403380:CDS	55.0%
	GTTAAAGCCTCTTCTGCCCC+TGG	+	tig0022956:2830-2849	Msa1403380:CDS	55.0%
	GAAACTTCCAGGGGCAGAAG+AGG	-	tig0022956:2840-2859	None:intergenic	55.0%
	GTTCTCTAGCATGTGGCCTC+TGG	+	tig0022956:3169-3188	Msa1403380:three_prime_UTR	55.0%
	GCATGTGGCCTCTGGGTATT+TGG	+	tig0022956:3177-3196	Msa1403380:three_prime_UTR	55.0%
	ATGCAGGTGGGAGAACTTGC+GGG	+	tig0022956:2011-2030	Msa1403380:CDS	55.0%
!!	GCCCCTATTCTTGGTGCTCT+TGG	+	tig0022956:2752-2771	Msa1403380:CDS	55.0%
	GTTAAAGCCTCTTCTGCCCC+TGG	+	tig0022956:2830-2849	Msa1403380:CDS	55.0%
	GAAACTTCCAGGGGCAGAAG+AGG	-	tig0022956:2840-2859	None:intergenic	55.0%
	GTTCTCTAGCATGTGGCCTC+TGG	+	tig0022956:3169-3188	Msa1403380:three_prime_UTR	55.0%
	GCATGTGGCCTCTGGGTATT+TGG	+	tig0022956:3177-3196	Msa1403380:three_prime_UTR	55.0%
	GGGAGAACTTGCGGGAATCG+CGG	+	tig0022956:2019-2038	Msa1403380:CDS	60.0%
	AGAACTTGCGGGAATCGCGG+TGG	+	tig0022956:2022-2041	Msa1403380:CDS	60.0%
	GAACTTGCGGGAATCGCGGT+GGG	+	tig0022956:2023-2042	Msa1403380:CDS	60.0%
	GGGAGAACTTGCGGGAATCG+CGG	+	tig0022956:2019-2038	Msa1403380:CDS	60.0%
	AGAACTTGCGGGAATCGCGG+TGG	+	tig0022956:2022-2041	Msa1403380:CDS	60.0%
	GAACTTGCGGGAATCGCGGT+GGG	+	tig0022956:2023-2042	Msa1403380:CDS	60.0%
!!	CTTGGTGCTCTTGGTGGGGC+AGG	+	tig0022956:2761-2780	Msa1403380:CDS	65.0%
!!	CTTGGTGCTCTTGGTGGGGC+AGG	+	tig0022956:2761-2780	Msa1403380:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0022956	gene	1923	3291	1923	ID=Msa1403380;Name=Msa1403380
tig0022956	mRNA	1923	3291	1923	ID=Msa1403380-mRNA-1;Parent=Msa1403380;Name=Msa1403380-mRNA-1;_AED=0.00;_eAED=0.00;_QI=88\|1\|1\|1\|1\|1\|2\|423\|91
tig0022956	exon	1923	2078	1923	ID=Msa1403380-mRNA-1:exon:19255;Parent=Msa1403380-mRNA-1
tig0022956	exon	2661	3291	2661	ID=Msa1403380-mRNA-1:exon:19256;Parent=Msa1403380-mRNA-1
tig0022956	five_prime_UTR	1923	2010	1923	ID=Msa1403380-mRNA-1:five_prime_utr;Parent=Msa1403380-mRNA-1
tig0022956	CDS	2011	2078	2011	ID=Msa1403380-mRNA-1:cds;Parent=Msa1403380-mRNA-1
tig0022956	CDS	2661	2868	2661	ID=Msa1403380-mRNA-1:cds;Parent=Msa1403380-mRNA-1
tig0022956	three_prime_UTR	2869	3291	2869	ID=Msa1403380-mRNA-1:three_prime_utr;Parent=Msa1403380-mRNA-1

Gene Sequence	>Msa1403380 ATGCAGGTGGGAGAACTTGCGGGAATCGCGGTGGGAGCCACTGTCATGCTCAACATACTCATTGCTGGGCCTGCAACTGGAGCTTCAATGAATCCAGTAAGAACACTAGGACCAGCAATTGCTGCAAACAACTACAAAGGCATATGGCTATATCTCATAGCCCCTATTCTTGGTGCTCTTGGTGGGGCAGGAGCTTACACTGCTGTCAAGCTTCCTGATGAAGAGTTTAACTCTGAGGTTAAAGCCTCTTCTGCCCCTGGAAGTTTCAGAAGATGA
Protein sequence	>Msa1403380 MQVGELAGIAVGATVMLNILIAGPATGASMNPVRTLGPAIAANNYKGIWLYLIAPILGALGGAGAYTAVKLPDEEFNSEVKASSAPGSFRR*