AlfalfaGEDB | Msa1404250

M. sativa cultivar zhongmu-4 / Msa1404250

KEGG: K15263
GO: -
PFAM: PF12874.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1404250	XP_013458440.1	84.014	294	22	1	1	269	1	294	2.76e-172	487

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1404250	sp\|O64571\|UBA1C_ARATH	31.220	205	88	7	1	164	1	193	8.06e-17	83.2
Msa1404250	sp\|O64571\|UBA1C_ARATH	44.578	83	44	1	53	135	207	287	1.42e-12	70.9
Msa1404250	sp\|O64571\|UBA1C_ARATH	50.794	63	31	0	58	120	142	204	5.05e-11	66.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1404250	A0A072UT04	84.014	294	22	1	1	269	1	294	1.32e-172	487

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1220900	Msa1404250	0.803746	3.008565e-49	-8.615850e-47
Msa1266190	Msa1404250	0.825991	3.465747e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1404250	MtrunA17_Chr4g0070801	84.014	294	22	1	1	269	1	294	2.54e-176	487

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1404250	AT2G19385.1	43.771	297	117	8	1	269	1	275	6.95e-57	184
Msa1404250	AT2G19380.1	31.220	205	88	7	1	164	1	193	8.20e-18	83.2
Msa1404250	AT2G19380.1	44.578	83	44	1	53	135	207	287	1.44e-13	70.9
Msa1404250	AT2G19380.1	50.794	63	31	0	58	120	142	204	5.14e-12	66.2

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Find 83 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCATTCACCGGTTTC+AGG	0.267736	tig0023052:-43823	None:intergenic
AAATGAAAATGAATTGAGCG+AGG	0.672838	tig0023052:+44306	Msa1404250:CDS
AAATTATCTGTCTGGACTAG+TGG	0.555342	tig0023052:-43695	None:intergenic
AACGTCCTCCGTGGTTCTGT+AGG	0.574054	tig0023052:+42774	Msa1404250:CDS
AACTGGCGTTAACCTGATTT+CGG	0.270078	tig0023052:-41162	None:intergenic
AAGGAAACCCTAGAATAGCT+TGG	0.579841	tig0023052:+41111	Msa1404250:five_prime_UTR
AAGGCTGCCAAGCTATTCTA+GGG	0.547202	tig0023052:-41118	None:intergenic
AAGTATGGTCCAAAAGGCCA+AGG	0.606114	tig0023052:+42668	Msa1404250:CDS
AATTCAACATACAAATTTCA+AGG	0.401997	tig0023052:-44002	None:intergenic
ACACTGTCTAGAACTAGCAT+TGG	0.542344	tig0023052:-44747	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTAATCTACCAATTTACCAC+GGG	-	tig0023052:41079-41098	None:intergenic	30.0%
CAACCTTAAAACTAAGACTA+TGG	+	tig0023052:41231-41250	Msa1404250:exon	30.0%
AAGCTACATAGCATAAACAA+CGG	-	tig0023052:41410-41429	None:intergenic	30.0%
TGGACAAAAATAAGGCAAAA+AGG	-	tig0023052:41515-41534	None:intergenic	30.0%
GGGTCCTAATCTATAATTAA+TGG	-	tig0023052:41535-41554	None:intergenic	30.0%
CTTATGAAAACAGCTTATTG+TGG	-	tig0023052:41604-41623	None:intergenic	30.0%
AAGCTATATGAACATGTCAT+AGG	+	tig0023052:41621-41640	Msa1404250:intron	30.0%
TAAGTACTTGTAAGACTGTT+GGG	-	tig0023052:41665-41684	None:intergenic	30.0%
ATAAGTACTTGTAAGACTGT+TGG	-	tig0023052:41666-41685	None:intergenic	30.0%
ACTAATTTGAGCTTACTTAC+CGG	-	tig0023052:41690-41709	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0023052	gene	41071	44783	41071	ID=Msa1404250;Name=Msa1404250
tig0023052	mRNA	41071	44783	41071	ID=Msa1404250-mRNA-1;Parent=Msa1404250;Name=Msa1404250-mRNA-1;_AED=0.08;_eAED=0.08;_QI=179\|0.75\|1\|1\|1\|1\|5\|275\|269
tig0023052	exon	41071	41336	41071	ID=Msa1404250-mRNA-1:exon:19682;Parent=Msa1404250-mRNA-1
tig0023052	exon	42665	42795	42665	ID=Msa1404250-mRNA-1:exon:19683;Parent=Msa1404250-mRNA-1
tig0023052	exon	43595	44012	43595	ID=Msa1404250-mRNA-1:exon:19684;Parent=Msa1404250-mRNA-1
tig0023052	exon	44232	44342	44232	ID=Msa1404250-mRNA-1:exon:19685;Parent=Msa1404250-mRNA-1
tig0023052	exon	44446	44783	44446	ID=Msa1404250-mRNA-1:exon:19686;Parent=Msa1404250-mRNA-1
tig0023052	five_prime_UTR	41071	41249	41071	ID=Msa1404250-mRNA-1:five_prime_utr;Parent=Msa1404250-mRNA-1
tig0023052	CDS	41250	41336	41250	ID=Msa1404250-mRNA-1:cds;Parent=Msa1404250-mRNA-1
tig0023052	CDS	42665	42795	42665	ID=Msa1404250-mRNA-1:cds;Parent=Msa1404250-mRNA-1
tig0023052	CDS	43595	44012	43595	ID=Msa1404250-mRNA-1:cds;Parent=Msa1404250-mRNA-1
tig0023052	CDS	44232	44342	44232	ID=Msa1404250-mRNA-1:cds;Parent=Msa1404250-mRNA-1
tig0023052	CDS	44446	44508	44446	ID=Msa1404250-mRNA-1:cds;Parent=Msa1404250-mRNA-1
tig0023052	three_prime_UTR	44509	44783	44509	ID=Msa1404250-mRNA-1:three_prime_utr;Parent=Msa1404250-mRNA-1

Gene Sequence

>Msa1404250

ATGGTTTGGTTTCAATGTGAAGATTGTGGAGACAACCTCAAAAAACCTAAGTTGCCCAATCACTTCCGATCGTGCTCCGCCAACAAGGAAAAGTATGGTCCAAAAGGCCAAGGAAAAACTTTGAATGCTGCAGCTGCCAAGCCTGTCAAAGATGGCAAACAAAGGCTAGTCGTTGATATTAATGTGGGCTTAGCTGAACGTCCTCCGTGGTTCTGTAGTCTTTGCAATACAAAAGCTACAAGTAAGCAAGCACTTCTTCTACATGCTGAAGGAAAGAAACACGGGGCAAAAGCCCGAGCGTTCCATGCTTCGAAGCAACCACTAGTCCAGACAGATAATTTTGCGCCTGATGCAAAGGATGCAGTGGAGACGGGTTCTAATGATATGGCAAAAGATGACAAAATTTCAGAGCAGCCAAAGTTGCACGAGTCTTCTGAACAAAATAACCTGAAACCGGTGAATGAAGTTTCTTCCGAAAAGAAAAAGAGGAAACATGAAGCATTAGAGGGTGACGTTATTAAAAAAAACAAGAATGACACATCAGTTGACGTGGAAAATGGGGAAGTATTTCAGGGCGAGAAGGCAAGAGAGAGGAAAATAAAGTGGAAGAAGTTTATCAAAGCAGCCTTGAAATTTCATCCTGATGGATTGAAGATGAAGAAATTGAGAAAAGTTGTCCTCAAAGCACTGCAGGAATCTAGCATTGTAGTAAATGAAAATGAATTGAGCGAGGCGATTGAGCAGAAGATAAACTCCAGCTCCAACTTTGCAGTTGAGAACAAGTATGTGCGATTAGTGGCCAAAGATTGA

Protein sequence

>Msa1404250

MVWFQCEDCGDNLKKPKLPNHFRSCSANKEKYGPKGQGKTLNAAAAKPVKDGKQRLVVDINVGLAERPPWFCSLCNTKATSKQALLLHAEGKKHGAKARAFHASKQPLVQTDNFAPDAKDAVETGSNDMAKDDKISEQPKLHESSEQNNLKPVNEVSSEKKKRKHEALEGDVIKKNKNDTSVDVENGEVFQGEKARERKIKWKKFIKAALKFHPDGLKMKKLRKVVLKALQESSIVVNENELSEAIEQKINSSSNFAVENKYVRLVAKD*