Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1404800 | MCI20292.1 | 80.303 | 66 | 12 | 1 | 1 | 65 | 1 | 66 | 4.08e-26 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1404800 | A0A392Q8S6 | 80.303 | 66 | 12 | 1 | 1 | 65 | 1 | 66 | 1.95e-26 | 100 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0638480 | Msa1404800 | 0.811571 | 6.550605e-51 | -8.615850e-47 |
| Msa1005870 | Msa1404800 | 0.800560 | 1.360917e-48 | -8.615850e-47 |
| Msa1092010 | Msa1404800 | 0.801398 | 9.174649e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1404800 | MtrunA17_Chr4g0064591 | 84.848 | 66 | 9 | 1 | 1 | 65 | 1 | 66 | 2.44e-26 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGCAGTCGCTGCCTTTG+TGG | 0.362337 | tig0023091:+24196 | Msa1404800:CDS |
| AATCTTCATTATGACAATAC+TGG | 0.378273 | tig0023091:+24070 | Msa1404800:CDS |
| AATGAAGATTGTGAAAACTA+TGG | 0.383706 | tig0023091:-24057 | None:intergenic |
| TCGCTGCCTTTGTGGCTACT+TGG | 0.383950 | tig0023091:+24204 | Msa1404800:CDS |
| TGCACTCCTGCGCTTTCCAT+TGG | 0.429044 | tig0023091:-24155 | None:intergenic |
| CCTGCGCTTTCCATTGGTGC+TGG | 0.456395 | tig0023091:-24149 | None:intergenic |
| GAAAACTATGGCGATCATAC+AGG | 0.490327 | tig0023091:-24045 | None:intergenic |
| GCGCTTTCCATTGGTGCTGG+TGG | 0.511292 | tig0023091:-24146 | None:intergenic |
| TGCAATTCCACCAGCACCAA+TGG | 0.564463 | tig0023091:+24139 | Msa1404800:CDS |
| CCAGCACCAATGGAAAGCGC+AGG | 0.581096 | tig0023091:+24149 | Msa1404800:CDS |
| GAAAGCGCAGGAGTGCACCT+TGG | 0.629476 | tig0023091:+24161 | Msa1404800:CDS |
| CAAGAGTAGCTGAGACACCA+AGG | 0.661901 | tig0023091:-24178 | None:intergenic |
| GATGAACCAAGTAGCCACAA+AGG | 0.668175 | tig0023091:-24210 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATGAAGATTGTGAAAACTA+TGG | - | tig0023091:24060-24079 | None:intergenic | 25.0% |
| ! | AATCTTCATTATGACAATAC+TGG | + | tig0023091:24070-24089 | Msa1404800:CDS | 25.0% |
| !! | TTTTGCTAAATGTGGTTTTG+CGG | + | tig0023091:24094-24113 | Msa1404800:CDS | 30.0% |
| !!! | ATACTGGCTTTTGCTAAATG+TGG | + | tig0023091:24086-24105 | Msa1404800:CDS | 35.0% |
| GAAAACTATGGCGATCATAC+AGG | - | tig0023091:24048-24067 | None:intergenic | 40.0% | |
| GATGAACCAAGTAGCCACAA+AGG | - | tig0023091:24213-24232 | None:intergenic | 45.0% | |
| TGCAATTCCACCAGCACCAA+TGG | + | tig0023091:24139-24158 | Msa1404800:CDS | 50.0% | |
| CAAGAGTAGCTGAGACACCA+AGG | - | tig0023091:24181-24200 | None:intergenic | 50.0% | |
| TGCACTCCTGCGCTTTCCAT+TGG | - | tig0023091:24158-24177 | None:intergenic | 55.0% | |
| TCTTGCAGTCGCTGCCTTTG+TGG | + | tig0023091:24196-24215 | Msa1404800:CDS | 55.0% | |
| TCGCTGCCTTTGTGGCTACT+TGG | + | tig0023091:24204-24223 | Msa1404800:CDS | 55.0% | |
| !! | GCGCTTTCCATTGGTGCTGG+TGG | - | tig0023091:24149-24168 | None:intergenic | 60.0% |
| CCTGCGCTTTCCATTGGTGC+TGG | - | tig0023091:24152-24171 | None:intergenic | 60.0% | |
| ! | CCAGCACCAATGGAAAGCGC+AGG | + | tig0023091:24149-24168 | Msa1404800:CDS | 60.0% |
| ! | GAAAGCGCAGGAGTGCACCT+TGG | + | tig0023091:24161-24180 | Msa1404800:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023091 | gene | 24038 | 24235 | 24038 | ID=Msa1404800;Name=Msa1404800 |
| tig0023091 | mRNA | 24038 | 24235 | 24038 | ID=Msa1404800-mRNA-1;Parent=Msa1404800;Name=Msa1404800-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|65 |
| tig0023091 | exon | 24038 | 24235 | 24038 | ID=Msa1404800-mRNA-1:exon:23790;Parent=Msa1404800-mRNA-1 |
| tig0023091 | CDS | 24038 | 24235 | 24038 | ID=Msa1404800-mRNA-1:cds;Parent=Msa1404800-mRNA-1 |
| Gene Sequence |
| Protein sequence |
