AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1408560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1408560 MtrunA17_Chr8g0378071 99.794 485 1 0 1 485 1 485 0.0 1006
Msa1408560 MtrunA17_Chr3g0121861 91.134 485 43 0 1 485 1 485 0.0 917
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1408560 AT4G13940.1 89.691 485 50 0 1 485 1 485 0.0 909
Msa1408560 AT3G23810.1 88.866 485 54 0 1 485 1 485 0.0 899
Msa1408560 AT4G13940.3 80.412 485 50 1 1 485 1 440 0.0 801
Msa1408560 AT4G13940.2 88.705 363 41 0 123 485 2 364 0.0 688
Msa1408560 AT4G13940.4 89.935 308 31 0 1 308 1 308 0.0 568

Find 217 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTACCAATTTATTATTA+TGG 0.169252 tig0023390:-47913 None:intergenic
TCGGGTCCATAATAATAAAT+TGG 0.218649 tig0023390:+47907 Msa1408560:five_prime_UTR
CCCAATCATGTTGATTATAT+TGG 0.239195 tig0023390:-50749 None:intergenic
GGCTCTCATGGAAGGTTTCC+AGG 0.251177 tig0023390:+49615 Msa1408560:CDS
GTGAAGAGATCCGGTGATTC+TGG 0.281598 tig0023390:-48265 None:intergenic
GTGGGTGTTGTCTGTGGATA+TGG 0.293422 tig0023390:+49505 Msa1408560:CDS
CGAGGGTCGTTTGATGAACT+TGG 0.298869 tig0023390:+49888 Msa1408560:CDS
GGATGAGAGTGGGTATAAAT+AGG 0.306001 tig0023390:-47996 None:intergenic
GTACTGAACGTGCTCTTGAC+TGG 0.316338 tig0023390:+48460 Msa1408560:CDS
GATGAGAGTGGGTATAAATA+GGG 0.321093 tig0023390:-47995 None:intergenic
TTGAAGAGTCTCACCCTTCC+AGG 0.334073 tig0023390:-48424 None:intergenic
GGTGATTCTGGCACCTTTGA+AGG 0.335113 tig0023390:-48253 None:intergenic
ATGAACTTGGGTTGTGCTAC+TGG 0.335401 tig0023390:+49901 Msa1408560:CDS
TTCATCTTACGATACCTCTT+AGG 0.348879 tig0023390:-48666 None:intergenic
ATGATGCCGGTGTTGGTCTC+AGG 0.349022 tig0023390:-49856 None:intergenic
GATCTTTCCCAAGCCGATTT+CGG 0.352407 tig0023390:+48153 Msa1408560:CDS
ATCGAAACCCTAACCGCTTT+AGG 0.353133 tig0023390:+48312 Msa1408560:CDS
ACCAATATAATCAACATGAT+TGG 0.355172 tig0023390:+50748 Msa1408560:three_prime_UTR
GGTTTGAATAATGCCTTGTT+TGG 0.355894 tig0023390:+50366 Msa1408560:three_prime_UTR
GCTACTGATGTTATGATTGC+TGG 0.358771 tig0023390:+49478 Msa1408560:CDS
TGATCCAATCTGTGCCCTTC+AGG 0.361459 tig0023390:+49594 Msa1408560:CDS
CTGTAGTGAGCGGGCTTGTA+TGG 0.374543 tig0023390:-50144 None:intergenic
CCTGTCGGTTTGAGGCTTGA+TGG 0.375154 tig0023390:-49824 None:intergenic
TTCTGGCACCTTTGAAGGGT+TGG 0.375945 tig0023390:-48248 None:intergenic
GAGTCCGGGCATCTCGATTT+CGG 0.376850 tig0023390:-48196 None:intergenic
TAACAGTTTGACAACTTGTA+TGG 0.382331 tig0023390:+49421 Msa1408560:intron
CTTCAGCACCTAAAGCGGTT+AGG 0.385562 tig0023390:-48320 None:intergenic
TTTCAGAATTGTGTTAGAAT+AGG 0.387492 tig0023390:+50266 Msa1408560:three_prime_UTR
CATGATGATGTCCTTGTTAC+CGG 0.390795 tig0023390:-49689 None:intergenic
TGTCATGTGAAGTGCTTGTT+TGG 0.391392 tig0023390:+50582 Msa1408560:three_prime_UTR
TACTGAACGTGCTCTTGACT+GGG 0.392027 tig0023390:+48461 Msa1408560:CDS
TCTGGCACCTTTGAAGGGTT+GGG 0.392418 tig0023390:-48247 None:intergenic
CGTGCTCTTGACTGGGGTCC+AGG 0.395326 tig0023390:+48468 Msa1408560:CDS
GCACTTCATCTTGGCAAGCT+TGG 0.402551 tig0023390:+50060 Msa1408560:CDS
ACAGCGCCGCCGTCTTCGCC+TGG 0.403265 tig0023390:+48406 Msa1408560:CDS
CTGTGCCCTTCAGGCTCTCA+TGG 0.405295 tig0023390:+49603 Msa1408560:CDS
CTGGCCGTGAGTACAAGGTT+AGG 0.409733 tig0023390:+48130 Msa1408560:CDS
GTTTGAATAATGCCTTGTTT+GGG 0.411404 tig0023390:+50367 Msa1408560:three_prime_UTR
CTTGTACTCACGGCCAGCTT+GGG 0.411653 tig0023390:-48124 None:intergenic
AGGCATTATTCAAACCCATT+TGG 0.412199 tig0023390:-50359 None:intergenic
GTCACGAGCGATGGCGGCGG+CGG 0.418277 tig0023390:-48385 None:intergenic
CGCCGCCGTCTTCGCCTGGA+AGG 0.420226 tig0023390:+48410 Msa1408560:CDS
TTTCGATGAGGACGGCGGTT+TGG 0.421389 tig0023390:-48296 None:intergenic
GACAATGAAATTGATATGTC+TGG 0.422786 tig0023390:+49769 Msa1408560:CDS
GAAGAGAGTGTGAGAGGTGA+AGG 0.422931 tig0023390:-48027 None:intergenic
TTGTGGAATGAGAGGAAATC+TGG 0.425669 tig0023390:+49982 Msa1408560:CDS
TTCAGCACCTAAAGCGGTTA+GGG 0.426813 tig0023390:-48319 None:intergenic
GGCTTGTATGGACCCTCAAC+TGG 0.426904 tig0023390:-50132 None:intergenic
TTGAGTTGAGAAAATGTTGC+AGG 0.426919 tig0023390:-48355 None:intergenic
CTTGACTGGGGTCCAGGTGG+TGG 0.427852 tig0023390:+48474 Msa1408560:CDS
ACGAGCTCCAGCTTGCTTCA+AGG 0.428897 tig0023390:-49554 None:intergenic
TGTAATGTTAACAGTGGGTG+GGG 0.430081 tig0023390:+50293 Msa1408560:three_prime_UTR
AATGCCATTGTTTGCAACAT+TGG 0.434082 tig0023390:+49739 Msa1408560:CDS
AGGAAGAGTGAGGATGAGAG+TGG 0.434136 tig0023390:-48007 None:intergenic
GTTGATCCACGAAGGTGTTA+AGG 0.434225 tig0023390:+48536 Msa1408560:CDS
TATAAATAGGGGTTTAAGTA+AGG 0.442694 tig0023390:-47983 None:intergenic
AGTTCGGCAGGACATGAGTC+CGG 0.444561 tig0023390:-48211 None:intergenic
CCAATATAATCAACATGATT+GGG 0.447307 tig0023390:+50749 Msa1408560:three_prime_UTR
ATGTCCTGCCGAACTGAGTT+CGG 0.451578 tig0023390:+48219 Msa1408560:CDS
TGATGTAATCAGCCTGTGAT+TGG 0.451589 tig0023390:-50104 None:intergenic
TTCTGTTGAATGAGACCAAA+TGG 0.452922 tig0023390:+50344 Msa1408560:three_prime_UTR
AGTGGGTGGGGAATGGTAAG+AGG 0.453877 tig0023390:+50305 Msa1408560:three_prime_UTR
TGTACTGTACCGGACTTGAT+CGG 0.455731 tig0023390:-47943 None:intergenic
CTTCAGCCTTAACACCTTCG+TGG 0.456708 tig0023390:-48542 None:intergenic
GTAAGAGGAAGGCAACCTAT+TGG 0.458244 tig0023390:+50320 Msa1408560:three_prime_UTR
CTCATTCAACAGAAACCAAT+AGG 0.462764 tig0023390:-50335 None:intergenic
GCCCTTCAGGCTCTCATGGA+AGG 0.462937 tig0023390:+49607 Msa1408560:CDS
GGGTTTAAGTAAGGAGTTGG+TGG 0.465915 tig0023390:-47974 None:intergenic
GCTGTTGCCTTGAAGCAAGC+TGG 0.466153 tig0023390:+49547 Msa1408560:CDS
CCTTGTACTCACGGCCAGCT+TGG 0.467412 tig0023390:-48125 None:intergenic
CGGCATCATTGTCTTGGCCG+AGG 0.467549 tig0023390:+49870 Msa1408560:CDS
TCCATGTTGTTTCTCATAGT+CGG 0.468700 tig0023390:+50875 Msa1408560:three_prime_UTR
GTACTGTACCGGACTTGATC+GGG 0.471218 tig0023390:-47942 None:intergenic
TGAGAGATGATGAGAGGTCG+AGG 0.471930 tig0023390:+50171 Msa1408560:exon
CATCATCAAGAGTCAGAACC+TGG 0.474414 tig0023390:-49633 None:intergenic
TACATCAGTGTGCCAGTTGA+GGG 0.474583 tig0023390:+50120 Msa1408560:CDS
GAGCGATGGCGGCGGCGGCG+TGG 0.480226 tig0023390:-48380 None:intergenic
AAAACCCACCTGTCGGTTTG+AGG 0.483314 tig0023390:-49832 None:intergenic
CAATACAATAATACAAATCA+TGG 0.484810 tig0023390:-50841 None:intergenic
GAGCAGGACATCACAAAACT+AGG 0.485567 tig0023390:-49928 None:intergenic
CCAAGCTGGCCGTGAGTACA+AGG 0.485905 tig0023390:+48125 Msa1408560:CDS
GTGCTTACTATCATTAGAGA+TGG 0.486117 tig0023390:+48630 Msa1408560:CDS
GAGTCGGCCGAAATCGGCTT+GGG 0.486792 tig0023390:-48160 None:intergenic
GTCTGTGGATATGGAGATGT+TGG 0.487898 tig0023390:+49514 Msa1408560:CDS
GATGAGGACGGCGGTTTGGA+TGG 0.488837 tig0023390:-48292 None:intergenic
AAGATGAAGGATAGACTTGT+TGG 0.490166 tig0023390:+48681 Msa1408560:CDS
GCCTAAGCATCTTGATGAGA+AGG 0.497581 tig0023390:+50032 Msa1408560:CDS
ATCTGGAAAGTATGAGAAGA+AGG 0.498460 tig0023390:+49999 Msa1408560:CDS
TTCAAAGGTGCCAGAATCAC+CGG 0.500231 tig0023390:+48255 Msa1408560:CDS
AGAGCCTGAAGGGCACAGAT+TGG 0.501229 tig0023390:-49598 None:intergenic
TGCTTACTATCATTAGAGAT+GGG 0.504002 tig0023390:+48631 Msa1408560:CDS
TAGGGGTTTAAGTAAGGAGT+TGG 0.504325 tig0023390:-47977 None:intergenic
TTGTAATGTTAACAGTGGGT+GGG 0.506432 tig0023390:+50292 Msa1408560:three_prime_UTR
TGGTCCGAACTCAGTTCGGC+AGG 0.506633 tig0023390:-48223 None:intergenic
AAGGTGGCTGCACTTCATCT+TGG 0.508177 tig0023390:+50051 Msa1408560:CDS
ATGGTGACGCGCTTCACACC+AGG 0.509074 tig0023390:-49805 None:intergenic
AGACTACTACTGGTGTTAAG+AGG 0.510864 tig0023390:+48715 Msa1408560:CDS
CGAGTCGGCCGAAATCGGCT+TGG 0.511178 tig0023390:-48161 None:intergenic
CATACAAGCCCGCTCACTAC+AGG 0.511416 tig0023390:+50145 Msa1408560:CDS
GGGATGGTCCGAACTCAGTT+CGG 0.513848 tig0023390:-48227 None:intergenic
GGCGCTGTCACGAGCGATGG+CGG 0.513966 tig0023390:-48391 None:intergenic
TGGCCGTGAGTACAAGGTTA+GGG 0.514832 tig0023390:+48131 Msa1408560:CDS
GAGAGATGATGAGAGGTCGA+GGG 0.518522 tig0023390:+50172 Msa1408560:exon
GATCTCGAGTCGGCCGAAAT+CGG 0.518878 tig0023390:-48166 None:intergenic
GTTCGGCAGGACATGAGTCC+GGG 0.520546 tig0023390:-48210 None:intergenic
TGTTAACAGTGGGTGGGGAA+TGG 0.520780 tig0023390:+50298 Msa1408560:three_prime_UTR
TCTGTTGAATGAGACCAAAT+GGG 0.523199 tig0023390:+50345 Msa1408560:three_prime_UTR
TGATGTTATGATTGCTGGAA+AGG 0.524488 tig0023390:+49483 Msa1408560:CDS
GTGATTCTGGCACCTTTGAA+GGG 0.527036 tig0023390:-48252 None:intergenic
GCACCTTTGAAGGGTTGGGA+TGG 0.527296 tig0023390:-48243 None:intergenic
GACATCTTTGTTACCACCAC+CGG 0.528009 tig0023390:+49670 Msa1408560:CDS
GAGGGTCGTTTGATGAACTT+GGG 0.529037 tig0023390:+49889 Msa1408560:CDS
CAAGACAATGATGCCGGTGT+TGG 0.531085 tig0023390:-49863 None:intergenic
GTGAGACTCTTCAAGAGTAT+TGG 0.531338 tig0023390:+48433 Msa1408560:CDS
CAACACCGGCATCATTGTCT+TGG 0.531574 tig0023390:+49864 Msa1408560:CDS
GAGTCGTTGACATTAATAGC+AGG 0.532386 tig0023390:-48774 None:intergenic
GCTGTCACGAGCGATGGCGG+CGG 0.534560 tig0023390:-48388 None:intergenic
TTTGTAATGTTAACAGTGGG+TGG 0.535225 tig0023390:+50291 Msa1408560:three_prime_UTR
GAGAGAGAAGAGAGTGTGAG+AGG 0.535411 tig0023390:-48033 None:intergenic
CACTTCCCGATGGTTTGATG+AGG 0.537448 tig0023390:+49455 Msa1408560:CDS
ATGAGAGTGGGTATAAATAG+GGG 0.538212 tig0023390:-47994 None:intergenic
GTCCTGCTCCTTCACCAACC+AGG 0.539452 tig0023390:+49942 Msa1408560:CDS
GATGTCCTTGTTACCGGTGG+TGG 0.541620 tig0023390:-49683 None:intergenic
GGAAGAGTGAGGATGAGAGT+GGG 0.542482 tig0023390:-48006 None:intergenic
TGATGCCGGTGTTGGTCTCA+GGG 0.542982 tig0023390:-49855 None:intergenic
CTTTAGGTGCTGAAGTCCGT+TGG 0.543805 tig0023390:+48328 Msa1408560:CDS
TTATCAGTAGAACTTGGATC+AGG 0.545116 tig0023390:-48591 None:intergenic
GGCATCATTGTCTTGGCCGA+GGG 0.546680 tig0023390:+49871 Msa1408560:CDS
GAAGAACTTTATGAGAAGAC+TGG 0.546774 tig0023390:+48561 Msa1408560:CDS
CCATCGTCAACAATCAAGTC+AGG 0.547026 tig0023390:-48498 None:intergenic
AGACTCTTCAAGAGTATTGG+TGG 0.548092 tig0023390:+48436 Msa1408560:CDS
TCAGCGTTATCAGTAGAACT+TGG 0.550511 tig0023390:-48597 None:intergenic
TAAGTAAGGAGTTGGTGGAG+TGG 0.552531 tig0023390:-47969 None:intergenic
ATCAAGTCAGGACCACCACC+TGG 0.553340 tig0023390:-48486 None:intergenic
TTACATCAGTGTGCCAGTTG+AGG 0.554609 tig0023390:+50119 Msa1408560:CDS
CACCAAGCTTTCCCAATCAC+AGG 0.556789 tig0023390:+50092 Msa1408560:CDS
CTCGGCCAAGACAATGATGC+CGG 0.557327 tig0023390:-49869 None:intergenic
GTAGCCCTCATCAAACCATC+GGG 0.557790 tig0023390:-49460 None:intergenic
GATGTAATCAGCCTGTGATT+GGG 0.561747 tig0023390:-50103 None:intergenic
AGCCTGTGATTGGGAAAGCT+TGG 0.562267 tig0023390:-50094 None:intergenic
TCATTGCTCAGCTTGAGTTG+TGG 0.563092 tig0023390:+49965 Msa1408560:CDS
CAAGTTCATCAAACGACCCT+CGG 0.565126 tig0023390:-49887 None:intergenic
GTTATGATTGCTGGAAAGGT+GGG 0.565954 tig0023390:+49487 Msa1408560:CDS
GGTGGGGAATGGTAAGAGGA+AGG 0.569401 tig0023390:+50309 Msa1408560:three_prime_UTR
AGTAGCCCTCATCAAACCAT+CGG 0.569406 tig0023390:-49461 None:intergenic
TCTCAATACCTGTAGTGAGC+GGG 0.569862 tig0023390:-50153 None:intergenic
ACCTTCCATGAGAGCCTGAA+GGG 0.569942 tig0023390:-49608 None:intergenic
TGACACAGCAATATCATACA+TGG 0.571677 tig0023390:-50559 None:intergenic
TGTTACCACCACCGGTAACA+AGG 0.571856 tig0023390:+49678 Msa1408560:CDS
CTTTATCAGATGCAAGCTAG+TGG 0.573265 tig0023390:+48738 Msa1408560:CDS
TGCCGTCACTCACTTCCCGA+TGG 0.573321 tig0023390:+49445 Msa1408560:CDS
GATGGTCATGTGAAGAGATC+CGG 0.575220 tig0023390:-48274 None:intergenic
GGTTGAAGACTGATCCTAAG+AGG 0.575943 tig0023390:+48652 Msa1408560:CDS
GGCGGCGCTGTCACGAGCGA+TGG 0.576329 tig0023390:-48394 None:intergenic
GACCTGGTTGGTGAAGGAGC+AGG 0.578362 tig0023390:-49944 None:intergenic
TAAACGCAGCGTTTGTGTGA+GGG 0.578863 tig0023390:-48062 None:intergenic
TAAGAGGTATCGTAAGATGA+AGG 0.581482 tig0023390:+48668 Msa1408560:CDS
CTCACCCTTCCAGGCGAAGA+CGG 0.582229 tig0023390:-48415 None:intergenic
TTGGCCGAAATCGAGATGCC+CGG 0.583401 tig0023390:+48192 Msa1408560:CDS
GGAAAGGTGGGTGTTGTCTG+TGG 0.584558 tig0023390:+49499 Msa1408560:CDS
AGCTTGAGTTGTGGAATGAG+AGG 0.585507 tig0023390:+49974 Msa1408560:CDS
ACCTTCTCATCAAGATGCTT+AGG 0.585589 tig0023390:-50033 None:intergenic
GCCGCCGTCTTCGCCTGGAA+GGG 0.586397 tig0023390:+48411 Msa1408560:CDS
TCATCATGGTTGACGACATG+AGG 0.586533 tig0023390:+49704 Msa1408560:CDS
CCATCAAGCCTCAAACCGAC+AGG 0.586572 tig0023390:+49824 Msa1408560:CDS
GGACCATCCCAACCCTTCAA+AGG 0.588552 tig0023390:+48240 Msa1408560:CDS
GTGACCAATGTTGCAAACAA+TGG 0.589198 tig0023390:-49743 None:intergenic
CGGTAACAAGGACATCATCA+TGG 0.591464 tig0023390:+49690 Msa1408560:CDS
AAAACAAGTAGTACAGTGCA+GGG 0.595636 tig0023390:-50989 None:intergenic
TGTTATGATTGCTGGAAAGG+TGG 0.598138 tig0023390:+49486 Msa1408560:CDS
AACCTTCCATGAGAGCCTGA+AGG 0.599013 tig0023390:-49609 None:intergenic
GGAGTGGAATTGTACTGTAC+CGG 0.599374 tig0023390:-47953 None:intergenic
TCAAGCCTCAAACCGACAGG+TGG 0.599721 tig0023390:+49827 Msa1408560:CDS
ACAGTAGAGAAAACCCAAGC+TGG 0.601142 tig0023390:+48111 Msa1408560:CDS
GATCCCTAACCTTGTACTCA+CGG 0.602852 tig0023390:-48134 None:intergenic
ACTTCCCGATGGTTTGATGA+GGG 0.603102 tig0023390:+49456 Msa1408560:CDS
CTAAACGCAGCGTTTGTGTG+AGG 0.605590 tig0023390:-48063 None:intergenic
AGCAATGACCTGGTTGGTGA+AGG 0.606777 tig0023390:-49950 None:intergenic
GTTTCTGAAGAGACTACTAC+TGG 0.608136 tig0023390:+48705 Msa1408560:CDS
ATCACATGTTACAATAACAT+CGG 0.608765 tig0023390:-50645 None:intergenic
CTCTCAATACCTGTAGTGAG+CGG 0.610233 tig0023390:-50154 None:intergenic
CGACTCTGTTACCAAGAGCA+AGG 0.612040 tig0023390:+48791 Msa1408560:CDS
CGGCCGACTCGAGATCGAGT+TGG 0.613698 tig0023390:+48173 Msa1408560:CDS
ACTCAAGCTGAGCAATGACC+TGG 0.617464 tig0023390:-49960 None:intergenic
AGCAGGACATCACAAAACTA+GGG 0.618545 tig0023390:-49927 None:intergenic
AACCATCGGGAAGTGAGTGA+CGG 0.619456 tig0023390:-49447 None:intergenic
TTTGAATAATGCCTTGTTTG+GGG 0.620215 tig0023390:+50368 Msa1408560:three_prime_UTR
ACCCTTCCAGGCGAAGACGG+CGG 0.621848 tig0023390:-48412 None:intergenic
GGTTAGGGTTTCGATGAGGA+CGG 0.624245 tig0023390:-48304 None:intergenic
CTCAGGGAAAACCCACCTGT+CGG 0.631363 tig0023390:-49839 None:intergenic
TGAGAGGTGAAGGAAGAGTG+AGG 0.632445 tig0023390:-48017 None:intergenic
AAATGTTGCAGGAACACCAA+CGG 0.640245 tig0023390:-48344 None:intergenic
GGATATGGAGATGTTGGAAA+GGG 0.640627 tig0023390:+49520 Msa1408560:CDS
TGACACACAATCCCCAAACA+AGG 0.645253 tig0023390:-50379 None:intergenic
TGGATATGGAGATGTTGGAA+AGG 0.645644 tig0023390:+49519 Msa1408560:CDS
CATATGATTATGAACAATGG+TGG 0.647553 tig0023390:+50818 Msa1408560:three_prime_UTR
CAAGCCTCAAACCGACAGGT+GGG 0.648729 tig0023390:+49828 Msa1408560:CDS
TAAGCATCTTGATGAGAAGG+TGG 0.649946 tig0023390:+50035 Msa1408560:CDS
ACGGACTTCAGCACCTAAAG+CGG 0.651771 tig0023390:-48325 None:intergenic
AGTCGTTGACATTAATAGCA+GGG 0.657078 tig0023390:-48773 None:intergenic
AGGTATTGAGAGATGATGAG+AGG 0.657461 tig0023390:+50165 Msa1408560:exon
GCTCTTGACTGGGGTCCAGG+TGG 0.660501 tig0023390:+48471 Msa1408560:CDS
TCTGGACTTGAGAACTACCC+TGG 0.662161 tig0023390:+49787 Msa1408560:CDS
CGGCCAACTCGATCTCGAGT+CGG 0.664458 tig0023390:-48176 None:intergenic
GCGCATATGATTATGAACAA+TGG 0.665806 tig0023390:+50815 Msa1408560:three_prime_UTR
ACTTTATGAGAAGACTGGTG+AGG 0.668763 tig0023390:+48566 Msa1408560:CDS
GACTTGATTGTTGACGATGG+TGG 0.670668 tig0023390:+48501 Msa1408560:CDS
CCTGACTTGATTGTTGACGA+TGG 0.671777 tig0023390:+48498 Msa1408560:CDS
TAGGGTTTCGATGAGGACGG+CGG 0.676084 tig0023390:-48301 None:intergenic
TATTATTGTATTGTGCTACG+AGG 0.677275 tig0023390:+50851 Msa1408560:three_prime_UTR
AAGCTGAGCAATGACCTGGT+TGG 0.688031 tig0023390:-49956 None:intergenic
TGGTGACGCGCTTCACACCA+GGG 0.688289 tig0023390:-49804 None:intergenic
ACTGAACGTGCTCTTGACTG+GGG 0.690263 tig0023390:+48462 Msa1408560:CDS
ACCGACTATGAGAAACAACA+TGG 0.708152 tig0023390:-50876 None:intergenic
GATGATGTCCTTGTTACCGG+TGG 0.708198 tig0023390:-49686 None:intergenic
AAGCGGTTAGGGTTTCGATG+AGG 0.719864 tig0023390:-48308 None:intergenic
GCTACTTTGTTGATCCACGA+AGG 0.723062 tig0023390:+48528 Msa1408560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAAATATTTTGATC+TGG + tig0023390:48857-48876 Msa1408560:intron 10.0%
!!! TTTTTAAAATATTTTGATCT+GGG + tig0023390:48858-48877 Msa1408560:intron 10.0%
!! TTCTTACCAATTTATTATTA+TGG - tig0023390:47916-47935 None:intergenic 15.0%
!! AAATGAAAATAAACAAATCT+AGG - tig0023390:48839-48858 None:intergenic 15.0%
!!! TTTTAAAATATTTTGATCTG+GGG + tig0023390:48859-48878 Msa1408560:intron 15.0%
!! GGTATTATTATTATTATTGT+TGG - tig0023390:50731-50750 None:intergenic 15.0%
!! TAGATATTGGATTTGATAAA+TGG + tig0023390:49169-49188 Msa1408560:intron 20.0%
!! GTTGGTCAAAAAAAATATAT+TGG - tig0023390:50713-50732 None:intergenic 20.0%
!! CAATACAATAATACAAATCA+TGG - tig0023390:50844-50863 None:intergenic 20.0%
! TATAAATAGGGGTTTAAGTA+AGG - tig0023390:47986-48005 None:intergenic 25.0%
! AAAAAAAAAAACCTTGCTCT+TGG - tig0023390:48805-48824 None:intergenic 25.0%
!!! TGAAATTGAGTATTTGGTTT+TGG + tig0023390:49070-49089 Msa1408560:intron 25.0%
! ATCTTGGATTTAACAAAGTT+AGG + tig0023390:49285-49304 Msa1408560:intron 25.0%
! TTACAAAACCATAGATCAAA+TGG - tig0023390:49404-49423 None:intergenic 25.0%
!!! AGAAAATGGGTTTTATGAAA+TGG + tig0023390:50199-50218 Msa1408560:three_prime_UTR 25.0%
!! TGATTTTTCATTTTCCTTGT+TGG + tig0023390:50224-50243 Msa1408560:three_prime_UTR 25.0%
!! ATTTTCCTTGTTGGATTTTT+TGG + tig0023390:50233-50252 Msa1408560:three_prime_UTR 25.0%
! TAATTCCAAAAAATCCAACA+AGG - tig0023390:50241-50260 None:intergenic 25.0%
! TTTCAGAATTGTGTTAGAAT+AGG + tig0023390:50266-50285 Msa1408560:three_prime_UTR 25.0%
!! GTTTTTGTAATGTTAACAGT+GGG + tig0023390:50288-50307 Msa1408560:three_prime_UTR 25.0%
!!! TCTCATTTTTCAAACATAGA+AGG - tig0023390:50432-50451 None:intergenic 25.0%
!! GGTTGAAATTTTGTATAACA+GGG - tig0023390:50627-50646 None:intergenic 25.0%
! ATCACATGTTACAATAACAT+CGG - tig0023390:50648-50667 None:intergenic 25.0%
! ACCAATATAATCAACATGAT+TGG + tig0023390:50748-50767 Msa1408560:three_prime_UTR 25.0%
! CCAATATAATCAACATGATT+GGG + tig0023390:50749-50768 Msa1408560:three_prime_UTR 25.0%
!!! CGTTTTTTTGTTGTTGTTTT+TGG + tig0023390:50944-50963 Msa1408560:three_prime_UTR 25.0%
TCGGGTCCATAATAATAAAT+TGG + tig0023390:47907-47926 Msa1408560:five_prime_UTR 30.0%
TGCTTACTATCATTAGAGAT+GGG + tig0023390:48631-48650 Msa1408560:CDS 30.0%
ATTTATGCTTCTTCATGTGT+TGG + tig0023390:48898-48917 Msa1408560:intron 30.0%
! TGTGTTGTTGTATGTTATTG+AGG + tig0023390:48946-48965 Msa1408560:intron 30.0%
TTTAGTGTGAAATTGAGTGT+TGG + tig0023390:48980-48999 Msa1408560:intron 30.0%
TTAGTGTGAAATTGAGTGTT+GGG + tig0023390:48981-49000 Msa1408560:intron 30.0%
TCATTCACAAAACTTCATTC+AGG - tig0023390:49037-49056 None:intergenic 30.0%
GTAGTGTGAAATTGAGTATT+TGG + tig0023390:49064-49083 Msa1408560:intron 30.0%
CAGGACATTTATCAAACAAT+TGG - tig0023390:49101-49120 None:intergenic 30.0%
TAAATGTCCTGAATGAAGTT+TGG + tig0023390:49110-49129 Msa1408560:intron 30.0%
AGTTAACTATGATGTGAACT+TGG + tig0023390:49233-49252 Msa1408560:intron 30.0%
! TGTGAACTTGGTTGTATTTT+TGG + tig0023390:49245-49264 Msa1408560:intron 30.0%
!!! ACTATAGCATGTTTTGATCT+TGG + tig0023390:49269-49288 Msa1408560:intron 30.0%
TTAACAAAGTTAGGTGTTTG+CGG + tig0023390:49294-49313 Msa1408560:intron 30.0%
!! TGAATTTAGTTCCTGGATTT+TGG + tig0023390:49338-49357 Msa1408560:intron 30.0%
TAACAGTTTGACAACTTGTA+TGG + tig0023390:49421-49440 Msa1408560:intron 30.0%
GACAATGAAATTGATATGTC+TGG + tig0023390:49769-49788 Msa1408560:CDS 30.0%
! GGTTTTTGTAATGTTAACAG+TGG + tig0023390:50287-50306 Msa1408560:three_prime_UTR 30.0%
!! GTTTGAATAATGCCTTGTTT+GGG + tig0023390:50367-50386 Msa1408560:three_prime_UTR 30.0%
!! TTTGAATAATGCCTTGTTTG+GGG + tig0023390:50368-50387 Msa1408560:three_prime_UTR 30.0%
! CGGTTGAAATTTTGTATAAC+AGG - tig0023390:50628-50647 None:intergenic 30.0%
! CCCAATCATGTTGATTATAT+TGG - tig0023390:50752-50771 None:intergenic 30.0%
CATATGATTATGAACAATGG+TGG + tig0023390:50818-50837 Msa1408560:three_prime_UTR 30.0%
TATTATTGTATTGTGCTACG+AGG + tig0023390:50851-50870 Msa1408560:three_prime_UTR 30.0%
AATAAAAACCCGATCAAGTC+CGG + tig0023390:47934-47953 Msa1408560:five_prime_UTR 35.0%
ATGAGAGTGGGTATAAATAG+GGG - tig0023390:47997-48016 None:intergenic 35.0%
GATGAGAGTGGGTATAAATA+GGG - tig0023390:47998-48017 None:intergenic 35.0%
!! TTGAGTTGAGAAAATGTTGC+AGG - tig0023390:48358-48377 None:intergenic 35.0%
GAAGAACTTTATGAGAAGAC+TGG + tig0023390:48561-48580 Msa1408560:CDS 35.0%
TTATCAGTAGAACTTGGATC+AGG - tig0023390:48594-48613 None:intergenic 35.0%
GTGCTTACTATCATTAGAGA+TGG + tig0023390:48630-48649 Msa1408560:CDS 35.0%
TTCATCTTACGATACCTCTT+AGG - tig0023390:48669-48688 None:intergenic 35.0%
!! TAAGAGGTATCGTAAGATGA+AGG + tig0023390:48668-48687 Msa1408560:CDS 35.0%
AAGATGAAGGATAGACTTGT+TGG + tig0023390:48681-48700 Msa1408560:CDS 35.0%
AGTCGTTGACATTAATAGCA+GGG - tig0023390:48776-48795 None:intergenic 35.0%
!! CTTCTTCATGTGTTGGTTTA+GGG + tig0023390:48905-48924 Msa1408560:intron 35.0%
!!! TGAAATTGAGTGTTGGGTTT+TGG + tig0023390:48987-49006 Msa1408560:intron 35.0%
ACACATACCAAACTTCATTC+AGG - tig0023390:49120-49139 None:intergenic 35.0%
TTCTGTAAGATACGTTTGTG+TGG + tig0023390:49206-49225 Msa1408560:intron 35.0%
! GATGATGTGAATTTAGTTCC+TGG + tig0023390:49331-49350 Msa1408560:intron 35.0%
! TCTGTTTGCCATTTGATCTA+TGG + tig0023390:49393-49412 Msa1408560:intron 35.0%
TGATGTTATGATTGCTGGAA+AGG + tig0023390:49483-49502 Msa1408560:CDS 35.0%
AATGCCATTGTTTGCAACAT+TGG + tig0023390:49739-49758 Msa1408560:CDS 35.0%
! ATCTGGAAAGTATGAGAAGA+AGG + tig0023390:49999-50018 Msa1408560:CDS 35.0%
TTTGTAATGTTAACAGTGGG+TGG + tig0023390:50291-50310 Msa1408560:three_prime_UTR 35.0%
TTGTAATGTTAACAGTGGGT+GGG + tig0023390:50292-50311 Msa1408560:three_prime_UTR 35.0%
CTCATTCAACAGAAACCAAT+AGG - tig0023390:50338-50357 None:intergenic 35.0%
TTCTGTTGAATGAGACCAAA+TGG + tig0023390:50344-50363 Msa1408560:three_prime_UTR 35.0%
TCTGTTGAATGAGACCAAAT+GGG + tig0023390:50345-50364 Msa1408560:three_prime_UTR 35.0%
AGGCATTATTCAAACCCATT+TGG - tig0023390:50362-50381 None:intergenic 35.0%
! GGTTTGAATAATGCCTTGTT+TGG + tig0023390:50366-50385 Msa1408560:three_prime_UTR 35.0%
TGACACAGCAATATCATACA+TGG - tig0023390:50562-50581 None:intergenic 35.0%
GCGCATATGATTATGAACAA+TGG + tig0023390:50815-50834 Msa1408560:three_prime_UTR 35.0%
!! TCCATGTTGTTTCTCATAGT+CGG + tig0023390:50875-50894 Msa1408560:three_prime_UTR 35.0%
!!! TTTGTTTTGTAGCGCTCTTT+TGG + tig0023390:50906-50925 Msa1408560:three_prime_UTR 35.0%
AAAACAAGTAGTACAGTGCA+GGG - tig0023390:50992-51011 None:intergenic 35.0%
AAAAACAAGTAGTACAGTGC+AGG - tig0023390:50993-51012 None:intergenic 35.0%
TAGGGGTTTAAGTAAGGAGT+TGG - tig0023390:47980-47999 None:intergenic 40.0%
GGATGAGAGTGGGTATAAAT+AGG - tig0023390:47999-48018 None:intergenic 40.0%
AAATGTTGCAGGAACACCAA+CGG - tig0023390:48347-48366 None:intergenic 40.0%
! GTGAGACTCTTCAAGAGTAT+TGG + tig0023390:48433-48452 Msa1408560:CDS 40.0%
!! AGACTCTTCAAGAGTATTGG+TGG + tig0023390:48436-48455 Msa1408560:CDS 40.0%
ACTTTATGAGAAGACTGGTG+AGG + tig0023390:48566-48585 Msa1408560:CDS 40.0%
TCAGCGTTATCAGTAGAACT+TGG - tig0023390:48600-48619 None:intergenic 40.0%
! GTTTCTGAAGAGACTACTAC+TGG + tig0023390:48705-48724 Msa1408560:CDS 40.0%
! AGACTACTACTGGTGTTAAG+AGG + tig0023390:48715-48734 Msa1408560:CDS 40.0%
CTTTATCAGATGCAAGCTAG+TGG + tig0023390:48738-48757 Msa1408560:CDS 40.0%
GAGTCGTTGACATTAATAGC+AGG - tig0023390:48777-48796 None:intergenic 40.0%
!! GCTTCTTCATGTGTTGGTTT+AGG + tig0023390:48904-48923 Msa1408560:intron 40.0%
GCTACTGATGTTATGATTGC+TGG + tig0023390:49478-49497 Msa1408560:CDS 40.0%
TGTTATGATTGCTGGAAAGG+TGG + tig0023390:49486-49505 Msa1408560:CDS 40.0%
GTTATGATTGCTGGAAAGGT+GGG + tig0023390:49487-49506 Msa1408560:CDS 40.0%
TGGATATGGAGATGTTGGAA+AGG + tig0023390:49519-49538 Msa1408560:CDS 40.0%
GGATATGGAGATGTTGGAAA+GGG + tig0023390:49520-49539 Msa1408560:CDS 40.0%
! CATGATGATGTCCTTGTTAC+CGG - tig0023390:49692-49711 None:intergenic 40.0%
GTGACCAATGTTGCAAACAA+TGG - tig0023390:49746-49765 None:intergenic 40.0%
AGCAGGACATCACAAAACTA+GGG - tig0023390:49930-49949 None:intergenic 40.0%
TTGTGGAATGAGAGGAAATC+TGG + tig0023390:49982-50001 Msa1408560:CDS 40.0%
ACCTTCTCATCAAGATGCTT+AGG - tig0023390:50036-50055 None:intergenic 40.0%
TAAGCATCTTGATGAGAAGG+TGG + tig0023390:50035-50054 Msa1408560:CDS 40.0%
GATGTAATCAGCCTGTGATT+GGG - tig0023390:50106-50125 None:intergenic 40.0%
TGATGTAATCAGCCTGTGAT+TGG - tig0023390:50107-50126 None:intergenic 40.0%
!! AGGTATTGAGAGATGATGAG+AGG + tig0023390:50165-50184 Msa1408560:exon 40.0%
AGGTCGAGGGAAAAAGAAAA+TGG + tig0023390:50185-50204 Msa1408560:three_prime_UTR 40.0%
GGTCGAGGGAAAAAGAAAAT+GGG + tig0023390:50186-50205 Msa1408560:three_prime_UTR 40.0%
TGTAATGTTAACAGTGGGTG+GGG + tig0023390:50293-50312 Msa1408560:three_prime_UTR 40.0%
! TGTCATGTGAAGTGCTTGTT+TGG + tig0023390:50582-50601 Msa1408560:three_prime_UTR 40.0%
ACCGACTATGAGAAACAACA+TGG - tig0023390:50879-50898 None:intergenic 40.0%
TGTACTGTACCGGACTTGAT+CGG - tig0023390:47946-47965 None:intergenic 45.0%
GGAGTGGAATTGTACTGTAC+CGG - tig0023390:47956-47975 None:intergenic 45.0%
!! TAAGTAAGGAGTTGGTGGAG+TGG - tig0023390:47972-47991 None:intergenic 45.0%
GGGTTTAAGTAAGGAGTTGG+TGG - tig0023390:47977-47996 None:intergenic 45.0%
TAAACGCAGCGTTTGTGTGA+GGG - tig0023390:48065-48084 None:intergenic 45.0%
ACAGTAGAGAAAACCCAAGC+TGG + tig0023390:48111-48130 Msa1408560:CDS 45.0%
GATCCCTAACCTTGTACTCA+CGG - tig0023390:48137-48156 None:intergenic 45.0%
! GATCTTTCCCAAGCCGATTT+CGG + tig0023390:48153-48172 Msa1408560:CDS 45.0%
GTGATTCTGGCACCTTTGAA+GGG - tig0023390:48255-48274 None:intergenic 45.0%
!! TTCAAAGGTGCCAGAATCAC+CGG + tig0023390:48255-48274 Msa1408560:CDS 45.0%
GATGGTCATGTGAAGAGATC+CGG - tig0023390:48277-48296 None:intergenic 45.0%
ATCGAAACCCTAACCGCTTT+AGG + tig0023390:48312-48331 Msa1408560:CDS 45.0%
TTCAGCACCTAAAGCGGTTA+GGG - tig0023390:48322-48341 None:intergenic 45.0%
TACTGAACGTGCTCTTGACT+GGG + tig0023390:48461-48480 Msa1408560:CDS 45.0%
CCATCGTCAACAATCAAGTC+AGG - tig0023390:48501-48520 None:intergenic 45.0%
! CCTGACTTGATTGTTGACGA+TGG + tig0023390:48498-48517 Msa1408560:CDS 45.0%
GACTTGATTGTTGACGATGG+TGG + tig0023390:48501-48520 Msa1408560:CDS 45.0%
! GCTACTTTGTTGATCCACGA+AGG + tig0023390:48528-48547 Msa1408560:CDS 45.0%
!! GTTGATCCACGAAGGTGTTA+AGG + tig0023390:48536-48555 Msa1408560:CDS 45.0%
GGTTGAAGACTGATCCTAAG+AGG + tig0023390:48652-48671 Msa1408560:CDS 45.0%
TGTGTTCGAATGATGCAGCT+AGG + tig0023390:49143-49162 Msa1408560:intron 45.0%
GTGTTCGAATGATGCAGCTA+GGG + tig0023390:49144-49163 Msa1408560:intron 45.0%
TGCAGCTAGGGTGTAGATAT+TGG + tig0023390:49156-49175 Msa1408560:intron 45.0%
!! TCCTGGATTTTGGTGTACAG+TGG + tig0023390:49348-49367 Msa1408560:intron 45.0%
ACCACTGTACACCAAAATCC+AGG - tig0023390:49352-49371 None:intergenic 45.0%
!! GGATTTTGGTGTACAGTGGT+AGG + tig0023390:49352-49371 Msa1408560:intron 45.0%
ACTTCCCGATGGTTTGATGA+GGG + tig0023390:49456-49475 Msa1408560:CDS 45.0%
AGTAGCCCTCATCAAACCAT+CGG - tig0023390:49464-49483 None:intergenic 45.0%
GTCTGTGGATATGGAGATGT+TGG + tig0023390:49514-49533 Msa1408560:CDS 45.0%
CATCATCAAGAGTCAGAACC+TGG - tig0023390:49636-49655 None:intergenic 45.0%
GACATCTTTGTTACCACCAC+CGG + tig0023390:49670-49689 Msa1408560:CDS 45.0%
CGGTAACAAGGACATCATCA+TGG + tig0023390:49690-49709 Msa1408560:CDS 45.0%
TCATCATGGTTGACGACATG+AGG + tig0023390:49704-49723 Msa1408560:CDS 45.0%
CAAGTTCATCAAACGACCCT+CGG - tig0023390:49890-49909 None:intergenic 45.0%
GAGGGTCGTTTGATGAACTT+GGG + tig0023390:49889-49908 Msa1408560:CDS 45.0%
! ATGAACTTGGGTTGTGCTAC+TGG + tig0023390:49901-49920 Msa1408560:CDS 45.0%
GAGCAGGACATCACAAAACT+AGG - tig0023390:49931-49950 None:intergenic 45.0%
! TCATTGCTCAGCTTGAGTTG+TGG + tig0023390:49965-49984 Msa1408560:CDS 45.0%
! AGCTTGAGTTGTGGAATGAG+AGG + tig0023390:49974-49993 Msa1408560:CDS 45.0%
GCCTAAGCATCTTGATGAGA+AGG + tig0023390:50032-50051 Msa1408560:CDS 45.0%
TTACATCAGTGTGCCAGTTG+AGG + tig0023390:50119-50138 Msa1408560:CDS 45.0%
TACATCAGTGTGCCAGTTGA+GGG + tig0023390:50120-50139 Msa1408560:CDS 45.0%
TCTCAATACCTGTAGTGAGC+GGG - tig0023390:50156-50175 None:intergenic 45.0%
CTCTCAATACCTGTAGTGAG+CGG - tig0023390:50157-50176 None:intergenic 45.0%
GTAAGAGGAAGGCAACCTAT+TGG + tig0023390:50320-50339 Msa1408560:three_prime_UTR 45.0%
TGACACACAATCCCCAAACA+AGG - tig0023390:50382-50401 None:intergenic 45.0%
GTACTGTACCGGACTTGATC+GGG - tig0023390:47945-47964 None:intergenic 50.0%
GGAAGAGTGAGGATGAGAGT+GGG - tig0023390:48009-48028 None:intergenic 50.0%
AGGAAGAGTGAGGATGAGAG+TGG - tig0023390:48010-48029 None:intergenic 50.0%
TGAGAGGTGAAGGAAGAGTG+AGG - tig0023390:48020-48039 None:intergenic 50.0%
! GAAGAGAGTGTGAGAGGTGA+AGG - tig0023390:48030-48049 None:intergenic 50.0%
! GAGAGAGAAGAGAGTGTGAG+AGG - tig0023390:48036-48055 None:intergenic 50.0%
CTAAACGCAGCGTTTGTGTG+AGG - tig0023390:48066-48085 None:intergenic 50.0%
TGGCCGTGAGTACAAGGTTA+GGG + tig0023390:48131-48150 Msa1408560:CDS 50.0%
ATGTCCTGCCGAACTGAGTT+CGG + tig0023390:48219-48238 Msa1408560:CDS 50.0%
! TCTGGCACCTTTGAAGGGTT+GGG - tig0023390:48250-48269 None:intergenic 50.0%
! TTCTGGCACCTTTGAAGGGT+TGG - tig0023390:48251-48270 None:intergenic 50.0%
GGTGATTCTGGCACCTTTGA+AGG - tig0023390:48256-48275 None:intergenic 50.0%
GTGAAGAGATCCGGTGATTC+TGG - tig0023390:48268-48287 None:intergenic 50.0%
GGTTAGGGTTTCGATGAGGA+CGG - tig0023390:48307-48326 None:intergenic 50.0%
AAGCGGTTAGGGTTTCGATG+AGG - tig0023390:48311-48330 None:intergenic 50.0%
CTTCAGCACCTAAAGCGGTT+AGG - tig0023390:48323-48342 None:intergenic 50.0%
ACGGACTTCAGCACCTAAAG+CGG - tig0023390:48328-48347 None:intergenic 50.0%
!! CTTTAGGTGCTGAAGTCCGT+TGG + tig0023390:48328-48347 Msa1408560:CDS 50.0%
TTGAAGAGTCTCACCCTTCC+AGG - tig0023390:48427-48446 None:intergenic 50.0%
GTACTGAACGTGCTCTTGAC+TGG + tig0023390:48460-48479 Msa1408560:CDS 50.0%
ACTGAACGTGCTCTTGACTG+GGG + tig0023390:48462-48481 Msa1408560:CDS 50.0%
CTTCAGCCTTAACACCTTCG+TGG - tig0023390:48545-48564 None:intergenic 50.0%
CGACTCTGTTACCAAGAGCA+AGG + tig0023390:48791-48810 Msa1408560:CDS 50.0%
AACCATCGGGAAGTGAGTGA+CGG - tig0023390:49450-49469 None:intergenic 50.0%
CACTTCCCGATGGTTTGATG+AGG + tig0023390:49455-49474 Msa1408560:CDS 50.0%
GTAGCCCTCATCAAACCATC+GGG - tig0023390:49463-49482 None:intergenic 50.0%
! GTGGGTGTTGTCTGTGGATA+TGG + tig0023390:49505-49524 Msa1408560:CDS 50.0%
TGATCCAATCTGTGCCCTTC+AGG + tig0023390:49594-49613 Msa1408560:CDS 50.0%
ACCTTCCATGAGAGCCTGAA+GGG - tig0023390:49611-49630 None:intergenic 50.0%
AACCTTCCATGAGAGCCTGA+AGG - tig0023390:49612-49631 None:intergenic 50.0%
TGTTACCACCACCGGTAACA+AGG + tig0023390:49678-49697 Msa1408560:CDS 50.0%
GATGATGTCCTTGTTACCGG+TGG - tig0023390:49689-49708 None:intergenic 50.0%
TCTGGACTTGAGAACTACCC+TGG + tig0023390:49787-49806 Msa1408560:CDS 50.0%
!! AAAACCCACCTGTCGGTTTG+AGG - tig0023390:49835-49854 None:intergenic 50.0%
! GTTTTCCCTGAGACCAACAC+CGG + tig0023390:49850-49869 Msa1408560:CDS 50.0%
CAAGACAATGATGCCGGTGT+TGG - tig0023390:49866-49885 None:intergenic 50.0%
CAACACCGGCATCATTGTCT+TGG + tig0023390:49864-49883 Msa1408560:CDS 50.0%
CGAGGGTCGTTTGATGAACT+TGG + tig0023390:49888-49907 Msa1408560:CDS 50.0%
!! AGCAATGACCTGGTTGGTGA+AGG - tig0023390:49953-49972 None:intergenic 50.0%
AAGCTGAGCAATGACCTGGT+TGG - tig0023390:49959-49978 None:intergenic 50.0%
ACTCAAGCTGAGCAATGACC+TGG - tig0023390:49963-49982 None:intergenic 50.0%
AAGGTGGCTGCACTTCATCT+TGG + tig0023390:50051-50070 Msa1408560:CDS 50.0%
GCACTTCATCTTGGCAAGCT+TGG + tig0023390:50060-50079 Msa1408560:CDS 50.0%
CACCAAGCTTTCCCAATCAC+AGG + tig0023390:50092-50111 Msa1408560:CDS 50.0%
AGCCTGTGATTGGGAAAGCT+TGG - tig0023390:50097-50116 None:intergenic 50.0%
TGAGAGATGATGAGAGGTCG+AGG + tig0023390:50171-50190 Msa1408560:exon 50.0%
GAGAGATGATGAGAGGTCGA+GGG + tig0023390:50172-50191 Msa1408560:exon 50.0%
TGTTAACAGTGGGTGGGGAA+TGG + tig0023390:50298-50317 Msa1408560:three_prime_UTR 50.0%
CTTGTACTCACGGCCAGCTT+GGG - tig0023390:48127-48146 None:intergenic 55.0%
CTGGCCGTGAGTACAAGGTT+AGG + tig0023390:48130-48149 Msa1408560:CDS 55.0%
GATCTCGAGTCGGCCGAAAT+CGG - tig0023390:48169-48188 None:intergenic 55.0%
TTGGCCGAAATCGAGATGCC+CGG + tig0023390:48192-48211 Msa1408560:CDS 55.0%
GAGTCCGGGCATCTCGATTT+CGG - tig0023390:48199-48218 None:intergenic 55.0%
AGTTCGGCAGGACATGAGTC+CGG - tig0023390:48214-48233 None:intergenic 55.0%
GGGATGGTCCGAACTCAGTT+CGG - tig0023390:48230-48249 None:intergenic 55.0%
GGACCATCCCAACCCTTCAA+AGG + tig0023390:48240-48259 Msa1408560:CDS 55.0%
! GCACCTTTGAAGGGTTGGGA+TGG - tig0023390:48246-48265 None:intergenic 55.0%
TTTCGATGAGGACGGCGGTT+TGG - tig0023390:48299-48318 None:intergenic 55.0%
TAGGGTTTCGATGAGGACGG+CGG - tig0023390:48304-48323 None:intergenic 55.0%
ATCAAGTCAGGACCACCACC+TGG - tig0023390:48489-48508 None:intergenic 55.0%
! GGAAAGGTGGGTGTTGTCTG+TGG + tig0023390:49499-49518 Msa1408560:CDS 55.0%
GCTGTTGCCTTGAAGCAAGC+TGG + tig0023390:49547-49566 Msa1408560:CDS 55.0%
! ACGAGCTCCAGCTTGCTTCA+AGG - tig0023390:49557-49576 None:intergenic 55.0%
! AGAGCCTGAAGGGCACAGAT+TGG - tig0023390:49601-49620 None:intergenic 55.0%
GGCTCTCATGGAAGGTTTCC+AGG + tig0023390:49615-49634 Msa1408560:CDS 55.0%
GATGTCCTTGTTACCGGTGG+TGG - tig0023390:49686-49705 None:intergenic 55.0%
!! CCTGTCGGTTTGAGGCTTGA+TGG - tig0023390:49827-49846 None:intergenic 55.0%
CCATCAAGCCTCAAACCGAC+AGG + tig0023390:49824-49843 Msa1408560:CDS 55.0%
TCAAGCCTCAAACCGACAGG+TGG + tig0023390:49827-49846 Msa1408560:CDS 55.0%
CAAGCCTCAAACCGACAGGT+GGG + tig0023390:49828-49847 Msa1408560:CDS 55.0%
CTCAGGGAAAACCCACCTGT+CGG - tig0023390:49842-49861 None:intergenic 55.0%
!! TGATGCCGGTGTTGGTCTCA+GGG - tig0023390:49858-49877 None:intergenic 55.0%
!! ATGATGCCGGTGTTGGTCTC+AGG - tig0023390:49859-49878 None:intergenic 55.0%
CTCGGCCAAGACAATGATGC+CGG - tig0023390:49872-49891 None:intergenic 55.0%
GGCATCATTGTCTTGGCCGA+GGG + tig0023390:49871-49890 Msa1408560:CDS 55.0%
GGCTTGTATGGACCCTCAAC+TGG - tig0023390:50135-50154 None:intergenic 55.0%
! CTGTAGTGAGCGGGCTTGTA+TGG - tig0023390:50147-50166 None:intergenic 55.0%
CATACAAGCCCGCTCACTAC+AGG + tig0023390:50145-50164 Msa1408560:CDS 55.0%
AGTGGGTGGGGAATGGTAAG+AGG + tig0023390:50305-50324 Msa1408560:three_prime_UTR 55.0%
GGTGGGGAATGGTAAGAGGA+AGG + tig0023390:50309-50328 Msa1408560:three_prime_UTR 55.0%
CCTTGTACTCACGGCCAGCT+TGG - tig0023390:48128-48147 None:intergenic 60.0%
CCAAGCTGGCCGTGAGTACA+AGG + tig0023390:48125-48144 Msa1408560:CDS 60.0%
! GAGTCGGCCGAAATCGGCTT+GGG - tig0023390:48163-48182 None:intergenic 60.0%
CGGCCAACTCGATCTCGAGT+CGG - tig0023390:48179-48198 None:intergenic 60.0%
GTTCGGCAGGACATGAGTCC+GGG - tig0023390:48213-48232 None:intergenic 60.0%
TGGTCCGAACTCAGTTCGGC+AGG - tig0023390:48226-48245 None:intergenic 60.0%
! GATGAGGACGGCGGTTTGGA+TGG - tig0023390:48295-48314 None:intergenic 60.0%
CTCACCCTTCCAGGCGAAGA+CGG - tig0023390:48418-48437 None:intergenic 60.0%
TGCCGTCACTCACTTCCCGA+TGG + tig0023390:49445-49464 Msa1408560:CDS 60.0%
CTGTGCCCTTCAGGCTCTCA+TGG + tig0023390:49603-49622 Msa1408560:CDS 60.0%
GCCCTTCAGGCTCTCATGGA+AGG + tig0023390:49607-49626 Msa1408560:CDS 60.0%
! TGGTGACGCGCTTCACACCA+GGG - tig0023390:49807-49826 None:intergenic 60.0%
! ATGGTGACGCGCTTCACACC+AGG - tig0023390:49808-49827 None:intergenic 60.0%
CGGCATCATTGTCTTGGCCG+AGG + tig0023390:49870-49889 Msa1408560:CDS 60.0%
GTCCTGCTCCTTCACCAACC+AGG + tig0023390:49942-49961 Msa1408560:CDS 60.0%
!! GACCTGGTTGGTGAAGGAGC+AGG - tig0023390:49947-49966 None:intergenic 60.0%
CGAGTCGGCCGAAATCGGCT+TGG - tig0023390:48164-48183 None:intergenic 65.0%
!! CGGCCGACTCGAGATCGAGT+TGG + tig0023390:48173-48192 Msa1408560:CDS 65.0%
ACCCTTCCAGGCGAAGACGG+CGG - tig0023390:48415-48434 None:intergenic 65.0%
CGTGCTCTTGACTGGGGTCC+AGG + tig0023390:48468-48487 Msa1408560:CDS 65.0%
GCTCTTGACTGGGGTCCAGG+TGG + tig0023390:48471-48490 Msa1408560:CDS 65.0%
CTTGACTGGGGTCCAGGTGG+TGG + tig0023390:48474-48493 Msa1408560:CDS 65.0%
! GCTGTCACGAGCGATGGCGG+CGG - tig0023390:48391-48410 None:intergenic 70.0%
GGCGCTGTCACGAGCGATGG+CGG - tig0023390:48394-48413 None:intergenic 70.0%
GCCGCCGTCTTCGCCTGGAA+GGG + tig0023390:48411-48430 Msa1408560:CDS 70.0%
! GTCACGAGCGATGGCGGCGG+CGG - tig0023390:48388-48407 None:intergenic 75.0%
GGCGGCGCTGTCACGAGCGA+TGG - tig0023390:48397-48416 None:intergenic 75.0%
ACAGCGCCGCCGTCTTCGCC+TGG + tig0023390:48406-48425 Msa1408560:CDS 75.0%
CGCCGCCGTCTTCGCCTGGA+AGG + tig0023390:48410-48429 Msa1408560:CDS 75.0%
!! GAGCGATGGCGGCGGCGGCG+TGG - tig0023390:48383-48402 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
tig0023390 gene 47898 51045 47898 ID=Msa1408560;Name=Msa1408560
tig0023390 mRNA 47898 51045 47898 ID=Msa1408560-mRNA-1;Parent=Msa1408560;Name=Msa1408560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=204|1|1|1|1|1|2|872|485
tig0023390 exon 47898 48812 47898 ID=Msa1408560-mRNA-1:exon:25620;Parent=Msa1408560-mRNA-1
tig0023390 exon 49427 51045 49427 ID=Msa1408560-mRNA-1:exon:25621;Parent=Msa1408560-mRNA-1
tig0023390 five_prime_UTR 47898 48101 47898 ID=Msa1408560-mRNA-1:five_prime_utr;Parent=Msa1408560-mRNA-1
tig0023390 CDS 48102 48812 48102 ID=Msa1408560-mRNA-1:cds;Parent=Msa1408560-mRNA-1
tig0023390 CDS 49427 50173 49427 ID=Msa1408560-mRNA-1:cds;Parent=Msa1408560-mRNA-1
tig0023390 three_prime_UTR 50174 51045 50174 ID=Msa1408560-mRNA-1:three_prime_utr;Parent=Msa1408560-mRNA-1
Gene Sequence

>Msa1408560

ATGTCTCTTACAGTAGAGAAAACCCAAGCTGGCCGTGAGTACAAGGTTAGGGATCTTTCCCAAGCCGATTTCGGCCGACTCGAGATCGAGTTGGCCGAAATCGAGATGCCCGGACTCATGTCCTGCCGAACTGAGTTCGGACCATCCCAACCCTTCAAAGGTGCCAGAATCACCGGATCTCTTCACATGACCATCCAAACCGCCGTCCTCATCGAAACCCTAACCGCTTTAGGTGCTGAAGTCCGTTGGTGTTCCTGCAACATTTTCTCAACTCAAGACCACGCCGCCGCCGCCATCGCTCGTGACAGCGCCGCCGTCTTCGCCTGGAAGGGTGAGACTCTTCAAGAGTATTGGTGGTGTACTGAACGTGCTCTTGACTGGGGTCCAGGTGGTGGTCCTGACTTGATTGTTGACGATGGTGGTGATGCTACTTTGTTGATCCACGAAGGTGTTAAGGCTGAAGAACTTTATGAGAAGACTGGTGAGGTTCCTGATCCAAGTTCTACTGATAACGCTGAGTTTCAGCTTGTGCTTACTATCATTAGAGATGGGTTGAAGACTGATCCTAAGAGGTATCGTAAGATGAAGGATAGACTTGTTGGTGTTTCTGAAGAGACTACTACTGGTGTTAAGAGGCTTTATCAGATGCAAGCTAGTGGAACTCTTTTGTTCCCTGCTATTAATGTCAACGACTCTGTTACCAAGAGCAAGTTTGACAACTTGTATGGATGCCGTCACTCACTTCCCGATGGTTTGATGAGGGCTACTGATGTTATGATTGCTGGAAAGGTGGGTGTTGTCTGTGGATATGGAGATGTTGGAAAGGGTTGTGCTGTTGCCTTGAAGCAAGCTGGAGCTCGTGTGATCGTTACTGAAATTGATCCAATCTGTGCCCTTCAGGCTCTCATGGAAGGTTTCCAGGTTCTGACTCTTGATGATGTTGTCTCTTATGCTGACATCTTTGTTACCACCACCGGTAACAAGGACATCATCATGGTTGACGACATGAGGAAAATGAAAAACAATGCCATTGTTTGCAACATTGGTCACTTTGACAATGAAATTGATATGTCTGGACTTGAGAACTACCCTGGTGTGAAGCGCGTCACCATCAAGCCTCAAACCGACAGGTGGGTTTTCCCTGAGACCAACACCGGCATCATTGTCTTGGCCGAGGGTCGTTTGATGAACTTGGGTTGTGCTACTGGACACCCTAGTTTTGTGATGTCCTGCTCCTTCACCAACCAGGTCATTGCTCAGCTTGAGTTGTGGAATGAGAGGAAATCTGGAAAGTATGAGAAGAAGGTTTATGTTTTGCCTAAGCATCTTGATGAGAAGGTGGCTGCACTTCATCTTGGCAAGCTTGGAGCTAAACTCACCAAGCTTTCCCAATCACAGGCTGATTACATCAGTGTGCCAGTTGAGGGTCCATACAAGCCCGCTCACTACAGGTATTGA
Protein sequence

>Msa1408560

MSLTVEKTQAGREYKVRDLSQADFGRLEIELAEIEMPGLMSCRTEFGPSQPFKGARITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDHAAAAIARDSAAVFAWKGETLQEYWWCTERALDWGPGGGPDLIVDDGGDATLLIHEGVKAEELYEKTGEVPDPSSTDNAEFQLVLTIIRDGLKTDPKRYRKMKDRLVGVSEETTTGVKRLYQMQASGTLLFPAINVNDSVTKSKFDNLYGCRHSLPDGLMRATDVMIAGKVGVVCGYGDVGKGCAVALKQAGARVIVTEIDPICALQALMEGFQVLTLDDVVSYADIFVTTTGNKDIIMVDDMRKMKNNAIVCNIGHFDNEIDMSGLENYPGVKRVTIKPQTDRWVFPETNTGIIVLAEGRLMNLGCATGHPSFVMSCSFTNQVIAQLELWNERKSGKYEKKVYVLPKHLDEKVAALHLGKLGAKLTKLSQSQADYISVPVEGPYKPAHYRY*