Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410110 | A0A392MB99 | 50.935 | 214 | 53 | 7 | 1 | 169 | 185 | 391 | 2.03e-46 | 168 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1410100 | Msa1410110 | 0.800109 | 1.681149e-48 | -8.615850e-47 |
| Msa0289250 | Msa1410110 | 0.807329 | 5.329890e-50 | -8.615850e-47 |
| Msa0289260 | Msa1410110 | 0.994494 | 1.034216e-207 | -8.615850e-47 |
| Msa0289330 | Msa1410110 | 0.839870 | 1.223234e-57 | -8.615850e-47 |
| Msa0327120 | Msa1410110 | 0.966430 | 6.548468e-126 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410110 | MtrunA17_Chr1g0160961 | 49.065 | 214 | 57 | 7 | 1 | 169 | 1108 | 1314 | 1.01e-45 | 159 |
| Msa1410110 | MtrunA17_Chr5g0419891 | 48.598 | 214 | 58 | 7 | 1 | 169 | 984 | 1190 | 4.89e-45 | 157 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTCTATCATCTCATTTC+AGG | 0.217727 | tig0023613:-61475 | Msa1410110:CDS |
| GTTGTCAATTCCTTGAAAAC+TGG | 0.305092 | tig0023613:+61138 | None:intergenic |
| TTGGACAGGACCTGGGGATA+TGG | 0.313019 | tig0023613:-61647 | Msa1410110:CDS |
| GGCAACTTCAAGCAAATTAG+TGG | 0.357784 | tig0023613:+61201 | None:intergenic |
| CATCTCATTTCAGGAGTAGA+TGG | 0.362782 | tig0023613:-61466 | Msa1410110:CDS |
| AAGACAACATGCACTTTACT+AGG | 0.368570 | tig0023613:-61496 | Msa1410110:CDS |
| AGGCCACTCTGATGCGCATT+TGG | 0.371819 | tig0023613:-61388 | Msa1410110:CDS |
| AACAAAGAAAAGTCCACATT+TGG | 0.394798 | tig0023613:+61180 | None:intergenic |
| TTCATATTGCTGCCACCTAC+AGG | 0.401617 | tig0023613:+61520 | None:intergenic |
| ACACTGCCTTGGACAGGACC+TGG | 0.477027 | tig0023613:-61655 | Msa1410110:CDS |
| GTCAATGATGCATGCTGAAT+AGG | 0.489388 | tig0023613:+61106 | None:intergenic |
| CACTGCCTTGGACAGGACCT+GGG | 0.509070 | tig0023613:-61654 | Msa1410110:CDS |
| GCATTTGGATACGATATATG+AGG | 0.530559 | tig0023613:-61373 | Msa1410110:CDS |
| GCAGCGGATTAACACTGCCT+TGG | 0.543231 | tig0023613:-61666 | Msa1410110:CDS |
| GAGTAGATGGTTGTCTGTAA+AGG | 0.545061 | tig0023613:-61453 | Msa1410110:CDS |
| CAAGCTGGTCCTCTAAAAGT+TGG | 0.547141 | tig0023613:-61238 | Msa1410110:CDS |
| GGATTAACACTGCCTTGGAC+AGG | 0.550002 | tig0023613:-61661 | Msa1410110:CDS |
| TTGCTTGAAGTTGCCAAATG+TGG | 0.554295 | tig0023613:-61193 | Msa1410110:CDS |
| GCAACTTCAAGCAAATTAGT+GGG | 0.566368 | tig0023613:+61202 | None:intergenic |
| GGCAGTGTTAATCCGCTGCA+TGG | 0.594322 | tig0023613:+61670 | None:intergenic |
| ATATCCCCAGGTCCTGTCCA+AGG | 0.597750 | tig0023613:+61649 | None:intergenic |
| GTAAAATCTAAAGCCGTGGA+TGG | 0.599176 | tig0023613:-61431 | Msa1410110:CDS |
| GATGCATGCTGAATAGGAGA+AGG | 0.600149 | tig0023613:+61112 | None:intergenic |
| TTTCACTATACCAAGCAAGC+TGG | 0.602071 | tig0023613:-61253 | Msa1410110:CDS |
| TCTATAGCAACCATATCCCC+AGG | 0.613424 | tig0023613:+61637 | None:intergenic |
| AAAGGTAAAATCTAAAGCCG+TGG | 0.614741 | tig0023613:-61435 | Msa1410110:CDS |
| GGTTGCTATAGAGCAAACAT+TGG | 0.621396 | tig0023613:-61626 | Msa1410110:CDS |
| AAAAGAACTAATGCCATCCA+CGG | 0.628505 | tig0023613:+61418 | None:intergenic |
| ACTGCCTTGGACAGGACCTG+GGG | 0.640697 | tig0023613:-61653 | Msa1410110:CDS |
| TACTTCAAAATAGAGAGACA+TGG | 0.684859 | tig0023613:+61593 | None:intergenic |
| AAACAACCTTCACCATGCAG+CGG | 0.695848 | tig0023613:-61682 | None:intergenic |
| TATCCAAATGCGCATCAGAG+TGG | 0.708943 | tig0023613:+61385 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTGTATACAAAGTTCA+AGG | + | tig0023613:61203-61222 | None:intergenic | 20.0% |
| !!! | CATTAGTTCTTTTAGCATTA+AGG | - | tig0023613:61362-61381 | Msa1410110:intron | 25.0% |
| ! | AGAAGTCTTCAATAATAGAA+TGG | - | tig0023613:61440-61459 | Msa1410110:CDS | 25.0% |
| ! | GAAGTCTTCAATAATAGAAT+GGG | - | tig0023613:61441-61460 | Msa1410110:CDS | 25.0% |
| TACTTCAAAATAGAGAGACA+TGG | + | tig0023613:61180-61199 | None:intergenic | 30.0% | |
| AAAAATTTCTCCGTAACAAG+AGG | - | tig0023613:61217-61236 | Msa1410110:CDS | 30.0% | |
| !!! | CTTTTTTACTCCTCTTGTTA+CGG | + | tig0023613:61230-61249 | None:intergenic | 30.0% |
| AACAAAGAAAAGTCCACATT+TGG | + | tig0023613:61593-61612 | None:intergenic | 30.0% | |
| !!! | TGTGGACTTTTCTTTGTTTT+TGG | - | tig0023613:61595-61614 | Msa1410110:CDS | 30.0% |
| AAGACAACATGCACTTTACT+AGG | - | tig0023613:61274-61293 | Msa1410110:intron | 35.0% | |
| GGATTCTATCATCTCATTTC+AGG | - | tig0023613:61295-61314 | Msa1410110:intron | 35.0% | |
| ! | AAAGGTAAAATCTAAAGCCG+TGG | - | tig0023613:61335-61354 | Msa1410110:intron | 35.0% |
| AAAAGAACTAATGCCATCCA+CGG | + | tig0023613:61355-61374 | None:intergenic | 35.0% | |
| GCATTTGGATACGATATATG+AGG | - | tig0023613:61397-61416 | Msa1410110:CDS | 35.0% | |
| AAACTGAACCAAGGAAAAAG+TGG | + | tig0023613:61492-61511 | None:intergenic | 35.0% | |
| GAAATGTTGAAACTGAACCA+AGG | + | tig0023613:61501-61520 | None:intergenic | 35.0% | |
| ! | TCTACACTACCAACTTTTAG+AGG | + | tig0023613:61544-61563 | None:intergenic | 35.0% |
| GCAACTTCAAGCAAATTAGT+GGG | + | tig0023613:61571-61590 | None:intergenic | 35.0% | |
| ! | GAAAGAATGTCCAGTTTTCA+AGG | - | tig0023613:61622-61641 | Msa1410110:CDS | 35.0% |
| GTTGTCAATTCCTTGAAAAC+TGG | + | tig0023613:61635-61654 | None:intergenic | 35.0% | |
| GGTTGCTATAGAGCAAACAT+TGG | - | tig0023613:61144-61163 | Msa1410110:CDS | 40.0% | |
| AGAGGAGTAAAAAAGCCTGT+AGG | - | tig0023613:61235-61254 | Msa1410110:CDS | 40.0% | |
| CATCTCATTTCAGGAGTAGA+TGG | - | tig0023613:61304-61323 | Msa1410110:intron | 40.0% | |
| GAGTAGATGGTTGTCTGTAA+AGG | - | tig0023613:61317-61336 | Msa1410110:intron | 40.0% | |
| ! | GTAAAATCTAAAGCCGTGGA+TGG | - | tig0023613:61339-61358 | Msa1410110:intron | 40.0% |
| TTTCACTATACCAAGCAAGC+TGG | - | tig0023613:61517-61536 | Msa1410110:CDS | 40.0% | |
| GGCAACTTCAAGCAAATTAG+TGG | + | tig0023613:61572-61591 | None:intergenic | 40.0% | |
| TTGCTTGAAGTTGCCAAATG+TGG | - | tig0023613:61577-61596 | Msa1410110:CDS | 40.0% | |
| GTCAATGATGCATGCTGAAT+AGG | + | tig0023613:61667-61686 | None:intergenic | 40.0% | |
| TCTATAGCAACCATATCCCC+AGG | + | tig0023613:61136-61155 | None:intergenic | 45.0% | |
| GGAGTAAAAAAGCCTGTAGG+TGG | - | tig0023613:61238-61257 | Msa1410110:CDS | 45.0% | |
| TTCATATTGCTGCCACCTAC+AGG | + | tig0023613:61253-61272 | None:intergenic | 45.0% | |
| TATCCAAATGCGCATCAGAG+TGG | + | tig0023613:61388-61407 | None:intergenic | 45.0% | |
| !! | AGGAAAAAGTGGACTGGAAG+TGG | + | tig0023613:61481-61500 | None:intergenic | 45.0% |
| !!! | CTTCCAGTCCACTTTTTCCT+TGG | - | tig0023613:61481-61500 | Msa1410110:CDS | 45.0% |
| GAACCAAGGAAAAAGTGGAC+TGG | + | tig0023613:61487-61506 | None:intergenic | 45.0% | |
| !! | TTTTAGAGGACCAGCTTGCT+TGG | + | tig0023613:61530-61549 | None:intergenic | 45.0% |
| ! | CAAGCTGGTCCTCTAAAAGT+TGG | - | tig0023613:61532-61551 | Msa1410110:CDS | 45.0% |
| GATGCATGCTGAATAGGAGA+AGG | + | tig0023613:61661-61680 | None:intergenic | 45.0% | |
| GGATTAACACTGCCTTGGAC+AGG | - | tig0023613:61109-61128 | Msa1410110:CDS | 50.0% | |
| !! | GCAGCGGATTAACACTGCCT+TGG | - | tig0023613:61104-61123 | Msa1410110:CDS | 55.0% |
| ATATCCCCAGGTCCTGTCCA+AGG | + | tig0023613:61124-61143 | None:intergenic | 55.0% | |
| TTGGACAGGACCTGGGGATA+TGG | - | tig0023613:61123-61142 | Msa1410110:CDS | 55.0% | |
| AGGCCACTCTGATGCGCATT+TGG | - | tig0023613:61382-61401 | Msa1410110:CDS | 55.0% | |
| ACACTGCCTTGGACAGGACC+TGG | - | tig0023613:61115-61134 | Msa1410110:CDS | 60.0% | |
| CACTGCCTTGGACAGGACCT+GGG | - | tig0023613:61116-61135 | Msa1410110:CDS | 60.0% | |
| ACTGCCTTGGACAGGACCTG+GGG | - | tig0023613:61117-61136 | Msa1410110:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023613 | gene | 61102 | 61690 | 61102 | ID=Msa1410110;Name=Msa1410110 |
| tig0023613 | mRNA | 61102 | 61690 | 61102 | ID=Msa1410110-mRNA-1;Parent=Msa1410110;Name=Msa1410110-mRNA-1;_AED=0.45;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|169 |
| tig0023613 | exon | 61363 | 61690 | 61363 | ID=Msa1410110-mRNA-1:exon:26378;Parent=Msa1410110-mRNA-1 |
| tig0023613 | exon | 61102 | 61283 | 61102 | ID=Msa1410110-mRNA-1:exon:26377;Parent=Msa1410110-mRNA-1 |
| tig0023613 | CDS | 61363 | 61690 | 61363 | ID=Msa1410110-mRNA-1:cds;Parent=Msa1410110-mRNA-1 |
| tig0023613 | CDS | 61102 | 61283 | 61102 | ID=Msa1410110-mRNA-1:cds;Parent=Msa1410110-mRNA-1 |
| Gene Sequence |
| Protein sequence |
