AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0289330


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0289330 MtrunA17_Chr1g0160961 49.065 214 57 7 1 169 1108 1314 1.01e-45 159
Msa0289330 MtrunA17_Chr5g0419891 48.598 214 58 7 1 169 984 1190 4.89e-45 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATTCTATCATCTCATTTC+AGG 0.217727 2_3:+35543514 Msa0289330:CDS
GTTGTCAATTCCTTGAAAAC+TGG 0.305092 2_3:-35543851 None:intergenic
TTGGACAGGACCTGGGGATA+TGG 0.313019 2_3:+35543342 Msa0289330:CDS
GGCAACTTCAAGCAAATTAG+TGG 0.357784 2_3:-35543788 None:intergenic
CATCTCATTTCAGGAGTAGA+TGG 0.362782 2_3:+35543523 Msa0289330:CDS
AAGACAACATGCACTTTACT+AGG 0.368570 2_3:+35543493 Msa0289330:CDS
AGGCCACTCTGATGCGCATT+TGG 0.371819 2_3:+35543601 Msa0289330:CDS
AACAAAGAAAAGTCCACATT+TGG 0.394798 2_3:-35543809 None:intergenic
TTCATATTGCTGCCACCTAC+AGG 0.401617 2_3:-35543469 None:intergenic
ACACTGCCTTGGACAGGACC+TGG 0.477027 2_3:+35543334 Msa0289330:CDS
GTCAATGATGCATGCTGAAT+AGG 0.489388 2_3:-35543883 None:intergenic
CACTGCCTTGGACAGGACCT+GGG 0.509070 2_3:+35543335 Msa0289330:CDS
GCATTTGGATACGATATATG+AGG 0.530559 2_3:+35543616 Msa0289330:CDS
GCAGCGGATTAACACTGCCT+TGG 0.543231 2_3:+35543323 Msa0289330:CDS
GAGTAGATGGTTGTCTGTAA+AGG 0.545061 2_3:+35543536 Msa0289330:CDS
CAAGCTGGTCCTCTAAAAGT+TGG 0.547141 2_3:+35543751 Msa0289330:CDS
GGATTAACACTGCCTTGGAC+AGG 0.550002 2_3:+35543328 Msa0289330:CDS
TTGCTTGAAGTTGCCAAATG+TGG 0.554295 2_3:+35543796 Msa0289330:CDS
GCAACTTCAAGCAAATTAGT+GGG 0.566368 2_3:-35543787 None:intergenic
GTTGCTATAGAGCAAACATT+GGG 0.568539 2_3:+35543364 Msa0289330:CDS
GGTTGCTATAGAGCAAACAT+TGG 0.591297 2_3:+35543363 Msa0289330:CDS
GGCAGTGTTAATCCGCTGCA+TGG 0.594322 2_3:-35543319 None:intergenic
ATATCCCCAGGTCCTGTCCA+AGG 0.597750 2_3:-35543340 None:intergenic
GTAAAATCTAAAGCCGTGGA+TGG 0.599176 2_3:+35543558 Msa0289330:CDS
GATGCATGCTGAATAGGAGA+AGG 0.600149 2_3:-35543877 None:intergenic
TTTCACTATACCAAGCAAGC+TGG 0.602071 2_3:+35543736 Msa0289330:CDS
TCTATAGCAACCATATCCCC+AGG 0.613424 2_3:-35543352 None:intergenic
AAAGGTAAAATCTAAAGCCG+TGG 0.614741 2_3:+35543554 Msa0289330:CDS
AAAAGAACTAATGCCATCCA+CGG 0.628505 2_3:-35543571 None:intergenic
ACTGCCTTGGACAGGACCTG+GGG 0.640697 2_3:+35543336 Msa0289330:CDS
TACTTCAAAATAGAGAGACA+TGG 0.684859 2_3:-35543396 None:intergenic
AAACAACCTTCACCATGCAG+CGG 0.695848 2_3:+35543307 None:intergenic
TATCCAAATGCGCATCAGAG+TGG 0.708943 2_3:-35543604 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTGTATACAAAGTTCA+AGG - chr2_3:35543422-35543441 None:intergenic 20.0%
!!! CATTAGTTCTTTTAGCATTA+AGG + chr2_3:35543581-35543600 Msa0289330:CDS 25.0%
! AGAAGTCTTCAATAATAGAA+TGG + chr2_3:35543659-35543678 Msa0289330:intron 25.0%
! GAAGTCTTCAATAATAGAAT+GGG + chr2_3:35543660-35543679 Msa0289330:intron 25.0%
TACTTCAAAATAGAGAGACA+TGG - chr2_3:35543399-35543418 None:intergenic 30.0%
AAAAATTTCTCCGTAACAAG+AGG + chr2_3:35543436-35543455 Msa0289330:CDS 30.0%
!!! CTTTTTTACTCCTCTTGTTA+CGG - chr2_3:35543449-35543468 None:intergenic 30.0%
AACAAAGAAAAGTCCACATT+TGG - chr2_3:35543812-35543831 None:intergenic 30.0%
!!! TGTGGACTTTTCTTTGTTTT+TGG + chr2_3:35543814-35543833 Msa0289330:CDS 30.0%
GTTGCTATAGAGCAAACATT+GGG + chr2_3:35543364-35543383 Msa0289330:CDS 35.0%
AAGACAACATGCACTTTACT+AGG + chr2_3:35543493-35543512 Msa0289330:CDS 35.0%
GGATTCTATCATCTCATTTC+AGG + chr2_3:35543514-35543533 Msa0289330:CDS 35.0%
! AAAGGTAAAATCTAAAGCCG+TGG + chr2_3:35543554-35543573 Msa0289330:CDS 35.0%
AAAAGAACTAATGCCATCCA+CGG - chr2_3:35543574-35543593 None:intergenic 35.0%
GCATTTGGATACGATATATG+AGG + chr2_3:35543616-35543635 Msa0289330:CDS 35.0%
AAACTGAACCAAGGAAAAAG+TGG - chr2_3:35543711-35543730 None:intergenic 35.0%
GAAATGTTGAAACTGAACCA+AGG - chr2_3:35543720-35543739 None:intergenic 35.0%
! TCTACACTACCAACTTTTAG+AGG - chr2_3:35543763-35543782 None:intergenic 35.0%
GCAACTTCAAGCAAATTAGT+GGG - chr2_3:35543790-35543809 None:intergenic 35.0%
! GAAAGAATGTCCAGTTTTCA+AGG + chr2_3:35543841-35543860 Msa0289330:CDS 35.0%
GTTGTCAATTCCTTGAAAAC+TGG - chr2_3:35543854-35543873 None:intergenic 35.0%
GGTTGCTATAGAGCAAACAT+TGG + chr2_3:35543363-35543382 Msa0289330:CDS 40.0%
AGAGGAGTAAAAAAGCCTGT+AGG + chr2_3:35543454-35543473 Msa0289330:CDS 40.0%
CATCTCATTTCAGGAGTAGA+TGG + chr2_3:35543523-35543542 Msa0289330:CDS 40.0%
GAGTAGATGGTTGTCTGTAA+AGG + chr2_3:35543536-35543555 Msa0289330:CDS 40.0%
! GTAAAATCTAAAGCCGTGGA+TGG + chr2_3:35543558-35543577 Msa0289330:CDS 40.0%
TTTCACTATACCAAGCAAGC+TGG + chr2_3:35543736-35543755 Msa0289330:CDS 40.0%
GGCAACTTCAAGCAAATTAG+TGG - chr2_3:35543791-35543810 None:intergenic 40.0%
TTGCTTGAAGTTGCCAAATG+TGG + chr2_3:35543796-35543815 Msa0289330:CDS 40.0%
GTCAATGATGCATGCTGAAT+AGG - chr2_3:35543886-35543905 None:intergenic 40.0%
TCTATAGCAACCATATCCCC+AGG - chr2_3:35543355-35543374 None:intergenic 45.0%
GGAGTAAAAAAGCCTGTAGG+TGG + chr2_3:35543457-35543476 Msa0289330:CDS 45.0%
TTCATATTGCTGCCACCTAC+AGG - chr2_3:35543472-35543491 None:intergenic 45.0%
TATCCAAATGCGCATCAGAG+TGG - chr2_3:35543607-35543626 None:intergenic 45.0%
!! AGGAAAAAGTGGACTGGAAG+TGG - chr2_3:35543700-35543719 None:intergenic 45.0%
!!! CTTCCAGTCCACTTTTTCCT+TGG + chr2_3:35543700-35543719 Msa0289330:intron 45.0%
GAACCAAGGAAAAAGTGGAC+TGG - chr2_3:35543706-35543725 None:intergenic 45.0%
!! TTTTAGAGGACCAGCTTGCT+TGG - chr2_3:35543749-35543768 None:intergenic 45.0%
! CAAGCTGGTCCTCTAAAAGT+TGG + chr2_3:35543751-35543770 Msa0289330:CDS 45.0%
GATGCATGCTGAATAGGAGA+AGG - chr2_3:35543880-35543899 None:intergenic 45.0%
GGATTAACACTGCCTTGGAC+AGG + chr2_3:35543328-35543347 Msa0289330:CDS 50.0%
!! GCAGCGGATTAACACTGCCT+TGG + chr2_3:35543323-35543342 Msa0289330:CDS 55.0%
ATATCCCCAGGTCCTGTCCA+AGG - chr2_3:35543343-35543362 None:intergenic 55.0%
TTGGACAGGACCTGGGGATA+TGG + chr2_3:35543342-35543361 Msa0289330:CDS 55.0%
AGGCCACTCTGATGCGCATT+TGG + chr2_3:35543601-35543620 Msa0289330:CDS 55.0%
ACACTGCCTTGGACAGGACC+TGG + chr2_3:35543334-35543353 Msa0289330:CDS 60.0%
CACTGCCTTGGACAGGACCT+GGG + chr2_3:35543335-35543354 Msa0289330:CDS 60.0%
ACTGCCTTGGACAGGACCTG+GGG + chr2_3:35543336-35543355 Msa0289330:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 35543321 35543909 35543321 ID=Msa0289330;Name=Msa0289330
chr2_3 mRNA 35543321 35543909 35543321 ID=Msa0289330-mRNA-1;Parent=Msa0289330;Name=Msa0289330-mRNA-1;_AED=0.45;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|169
chr2_3 exon 35543321 35543648 35543321 ID=Msa0289330-mRNA-1:exon:11514;Parent=Msa0289330-mRNA-1
chr2_3 exon 35543728 35543909 35543728 ID=Msa0289330-mRNA-1:exon:11515;Parent=Msa0289330-mRNA-1
chr2_3 CDS 35543321 35543648 35543321 ID=Msa0289330-mRNA-1:cds;Parent=Msa0289330-mRNA-1
chr2_3 CDS 35543728 35543909 35543728 ID=Msa0289330-mRNA-1:cds;Parent=Msa0289330-mRNA-1
Gene Sequence

>Msa0289330

ATGCAGCGGATTAACACTGCCTTGGACAGGACCTGGGGATATGGTTGCTATAGAGCAAACATTGGGTCTATTGTTCCATGTCTCTCTATTTTGAAGTACCTTGAACTTTGTATACAAAAATTTCTCCGTAACAAGAGGAGTAAAAAAGCCTGTAGGTGGCAGCAATATGAAGAAGACAACATGCACTTTACTAGGATTCTATCATCTCATTTCAGGAGTAGATGGTTGTCTGTAAAGGTAAAATCTAAAGCCGTGGATGGCATTAGTTCTTTTAGCATTAAGGCCACTCTGATGCGCATTTGGATACGATATATGAGGACTCAGACATTTTCAACATTTCACTATACCAAGCAAGCTGGTCCTCTAAAAGTTGGTAGTGTAGATGATCCCACTAATTTGCTTGAAGTTGCCAAATGTGGACTTTTCTTTGTTTTTGGCATTGAAAGAATGTCCAGTTTTCAAGGAATTGACAACATTCCTTCTCCTATTCAGCATGCATCATTGACATGA

Protein sequence

>Msa0289330

MQRINTALDRTWGYGCYRANIGSIVPCLSILKYLELCIQKFLRNKRSKKACRWQQYEEDNMHFTRILSSHFRSRWLSVKVKSKAVDGISSFSIKATLMRIWIRYMRTQTFSTFHYTKQAGPLKVGSVDDPTNLLEVAKCGLFFVFGIERMSSFQGIDNIPSPIQHASLT*