Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410340 | G7JKL5 | 72.727 | 44 | 12 | 0 | 28 | 71 | 4 | 47 | 4.47e-11 | 63.9 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010710 | Msa1410340 | 0.809799 | 1.582340e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410340 | MtrunA17_Chr4g0005211 | 72.727 | 44 | 12 | 0 | 28 | 71 | 4 | 47 | 3.61e-14 | 62.4 |
| Msa1410340 | MtrunA17_Chr3g0122061 | 48.750 | 80 | 28 | 2 | 1 | 72 | 2 | 76 | 3.27e-13 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTTGACTACTATGTTATT+TGG | 0.301353 | tig0023648:+82398 | Msa1410340:CDS |
| CCTTCTGATCTAGACCTATA+TGG | 0.343204 | tig0023648:+82261 | Msa1410340:CDS |
| TCGATCAGAACTTTCTCATT+CGG | 0.393624 | tig0023648:+82377 | Msa1410340:CDS |
| GTCGGTGAGTCTTCAACGGT+TGG | 0.418675 | tig0023648:+82352 | Msa1410340:CDS |
| TATTTGGTGGTTTGTCTATG+TGG | 0.462582 | tig0023648:+82414 | Msa1410340:CDS |
| TTGACTACTATGTTATTTGG+TGG | 0.492151 | tig0023648:+82401 | Msa1410340:CDS |
| TCGGTGAGTCTTCAACGGTT+GGG | 0.493764 | tig0023648:+82353 | Msa1410340:CDS |
| GTTGCAAAGGTGATTCGTTA+AGG | 0.504875 | tig0023648:+82494 | Msa1410340:CDS |
| CACCATCGCCTCCTCCATAT+AGG | 0.513129 | tig0023648:-82275 | None:intergenic |
| GACCTATATGGAGGAGGCGA+TGG | 0.524738 | tig0023648:+82273 | Msa1410340:CDS |
| CCATATAGGTCTAGATCAGA+AGG | 0.542876 | tig0023648:-82261 | None:intergenic |
| AGGAGGCGATGGTGTGATTG+TGG | 0.547588 | tig0023648:+82284 | Msa1410340:CDS |
| TTTGTGGTGTGAGGAGGTGT+CGG | 0.556495 | tig0023648:+82334 | Msa1410340:CDS |
| CGATGGTGTTGCTGTTGTGA+AGG | 0.574601 | tig0023648:+82439 | Msa1410340:CDS |
| TGGTGTTGCTGTTGTGAAGG+TGG | 0.575806 | tig0023648:+82442 | Msa1410340:CDS |
| GGTTTGTCTATGTGGATCGA+TGG | 0.596675 | tig0023648:+82422 | Msa1410340:CDS |
| GATCTAGACCTATATGGAGG+AGG | 0.597154 | tig0023648:+82267 | Msa1410340:CDS |
| AGGTGTCGGTGAGTCTTCAA+CGG | 0.623839 | tig0023648:+82348 | Msa1410340:CDS |
| TCTGATCTAGACCTATATGG+AGG | 0.685762 | tig0023648:+82264 | Msa1410340:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GGTTTGACTACTATGTTATT+TGG | + | tig0023648:82398-82417 | Msa1410340:CDS | 30.0% | |
| ! | TTGACTACTATGTTATTTGG+TGG | + | tig0023648:82401-82420 | Msa1410340:CDS | 30.0% |
| TCGATCAGAACTTTCTCATT+CGG | + | tig0023648:82377-82396 | Msa1410340:CDS | 35.0% | |
| ! | TATTTGGTGGTTTGTCTATG+TGG | + | tig0023648:82414-82433 | Msa1410340:CDS | 35.0% |
| !!! | TGTTTGGTGTTTTTGTGTGG+TGG | + | tig0023648:82232-82251 | Msa1410340:CDS | 40.0% |
| CCATATAGGTCTAGATCAGA+AGG | - | tig0023648:82264-82283 | None:intergenic | 40.0% | |
| CCTTCTGATCTAGACCTATA+TGG | + | tig0023648:82261-82280 | Msa1410340:CDS | 40.0% | |
| TCTGATCTAGACCTATATGG+AGG | + | tig0023648:82264-82283 | Msa1410340:CDS | 40.0% | |
| ! | GGTTGTTGTTGTTGTTGCAA+AGG | + | tig0023648:82481-82500 | Msa1410340:CDS | 40.0% |
| GATCTAGACCTATATGGAGG+AGG | + | tig0023648:82267-82286 | Msa1410340:CDS | 45.0% | |
| GGTTTGTCTATGTGGATCGA+TGG | + | tig0023648:82422-82441 | Msa1410340:CDS | 45.0% | |
| GATGGTGTGATTGTGGTGTG+AGG | + | tig0023648:82291-82310 | Msa1410340:intron | 50.0% | |
| !! | GATGTAGGCCACGCTTTTTG+TGG | + | tig0023648:82318-82337 | Msa1410340:intron | 50.0% |
| TCCTCACACCACAAAAAGCG+TGG | - | tig0023648:82329-82348 | None:intergenic | 50.0% | |
| !! | ACGCTTTTTGTGGTGTGAGG+AGG | + | tig0023648:82328-82347 | Msa1410340:CDS | 50.0% |
| ! | TTTGTGGTGTGAGGAGGTGT+CGG | + | tig0023648:82334-82353 | Msa1410340:CDS | 50.0% |
| !! | AGGTGTCGGTGAGTCTTCAA+CGG | + | tig0023648:82348-82367 | Msa1410340:CDS | 50.0% |
| TCGGTGAGTCTTCAACGGTT+GGG | + | tig0023648:82353-82372 | Msa1410340:CDS | 50.0% | |
| !! | CGATGGTGTTGCTGTTGTGA+AGG | + | tig0023648:82439-82458 | Msa1410340:CDS | 50.0% |
| !! | TGGTGTTGCTGTTGTGAAGG+TGG | + | tig0023648:82442-82461 | Msa1410340:CDS | 50.0% |
| !!! | GGTGGATTTTGTTGTTGCGC+TGG | + | tig0023648:82460-82479 | Msa1410340:CDS | 50.0% |
| GACCTATATGGAGGAGGCGA+TGG | + | tig0023648:82273-82292 | Msa1410340:CDS | 55.0% | |
| CACCATCGCCTCCTCCATAT+AGG | - | tig0023648:82278-82297 | None:intergenic | 55.0% | |
| AGGAGGCGATGGTGTGATTG+TGG | + | tig0023648:82284-82303 | Msa1410340:CDS | 55.0% | |
| GGTGTGATTGTGGTGTGAGG+TGG | + | tig0023648:82294-82313 | Msa1410340:intron | 55.0% | |
| ! | GTGGTGTGAGGTGGTGATGT+AGG | + | tig0023648:82303-82322 | Msa1410340:intron | 55.0% |
| !! | GCCACGCTTTTTGTGGTGTG+AGG | + | tig0023648:82325-82344 | Msa1410340:CDS | 55.0% |
| GTCGGTGAGTCTTCAACGGT+TGG | + | tig0023648:82352-82371 | Msa1410340:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023648 | gene | 82231 | 82514 | 82231 | ID=Msa1410340;Name=Msa1410340 |
| tig0023648 | mRNA | 82231 | 82514 | 82231 | ID=Msa1410340-mRNA-1;Parent=Msa1410340;Name=Msa1410340-mRNA-1;_AED=0.31;_eAED=0.53;_QI=0|0|0|1|0|0.5|2|0|88 |
| tig0023648 | exon | 82231 | 82307 | 82231 | ID=Msa1410340-mRNA-1:exon:26497;Parent=Msa1410340-mRNA-1 |
| tig0023648 | exon | 82325 | 82514 | 82325 | ID=Msa1410340-mRNA-1:exon:26498;Parent=Msa1410340-mRNA-1 |
| tig0023648 | CDS | 82231 | 82307 | 82231 | ID=Msa1410340-mRNA-1:cds;Parent=Msa1410340-mRNA-1 |
| tig0023648 | CDS | 82325 | 82514 | 82325 | ID=Msa1410340-mRNA-1:cds;Parent=Msa1410340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
