AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1413650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413650 MtrunA17_Chr1g0213811 96.169 261 5 2 55 310 21 281 0.0 518
Msa1413650 MtrunA17_Chr5g0406051 52.444 225 91 4 89 305 67 283 4.62e-71 221
Msa1413650 MtrunA17_Chr3g0143381 52.607 211 82 3 105 304 70 273 1.33e-69 218
Msa1413650 MtrunA17_Chr8g0371221 53.110 209 86 3 105 305 68 272 3.91e-69 216
Msa1413650 MtrunA17_Chr8g0371281 50.962 208 90 3 105 304 67 270 2.35e-67 211
Msa1413650 MtrunA17_Chr8g0371251 49.519 208 93 3 105 304 69 272 9.21e-64 202
Msa1413650 MtrunA17_Chr6g0452281 48.558 208 90 3 107 304 54 254 4.98e-61 196
Msa1413650 MtrunA17_Chr4g0066531 47.418 213 96 3 103 304 36 243 2.85e-59 191
Msa1413650 MtrunA17_Chr8g0371231 50.249 201 88 3 113 305 1 197 9.96e-59 187
Msa1413650 MtrunA17_Chr2g0286811 45.370 216 100 3 102 304 38 248 6.95e-56 183
Msa1413650 MtrunA17_Chr3g0143361 49.398 166 82 1 139 304 4 167 7.01e-46 154
Msa1413650 MtrunA17_Chr4g0053801 38.298 235 134 4 75 305 62 289 1.66e-42 148
Msa1413650 MtrunA17_Chr5g0401481 35.094 265 145 6 65 309 50 307 3.44e-42 147
Msa1413650 MtrunA17_Chr8g0371241 44.000 175 68 4 140 305 4 157 3.93e-40 138
Msa1413650 MtrunA17_Chr3g0143371 47.403 154 78 2 136 288 1 152 8.44e-37 129
Msa1413650 MtrunA17_Chr3g0143351 44.970 169 84 3 139 304 4 166 1.11e-34 124
Msa1413650 MtrunA17_Chr6g0470551 34.500 200 111 5 107 304 57 238 7.98e-30 115
Msa1413650 MtrunA17_Chr3g0098671 53.448 58 26 1 188 245 31 87 1.19e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1413650 AT2G43080.1 67.939 262 72 2 55 306 20 279 1.54e-132 378
Msa1413650 AT1G20270.1 57.212 208 77 3 105 304 77 280 7.34e-72 224
Msa1413650 AT4G35810.1 55.238 210 78 4 105 304 81 284 3.56e-70 219
Msa1413650 AT4G35810.2 55.238 210 78 4 105 304 81 284 3.56e-70 219
Msa1413650 AT5G66060.1 48.963 241 98 4 76 305 57 283 5.94e-69 216
Msa1413650 AT2G17720.1 52.857 210 83 4 105 304 81 284 2.08e-66 210
Msa1413650 AT3G28480.1 39.615 260 133 5 62 304 7 259 1.70e-57 188
Msa1413650 AT2G43080.2 56.707 164 59 2 55 208 20 181 1.26e-56 181
Msa1413650 AT3G28490.1 47.087 206 97 4 107 304 35 236 4.28e-56 183
Msa1413650 AT3G06300.1 46.698 212 99 3 103 304 37 244 4.91e-56 184
Msa1413650 AT5G18900.1 45.070 213 101 3 103 304 36 243 3.54e-54 179
Msa1413650 AT3G28480.2 36.842 266 140 6 62 304 7 267 2.43e-47 162
Msa1413650 AT5G66060.2 47.368 190 78 4 76 254 57 235 3.69e-45 154
Msa1413650 AT1G20270.2 59.055 127 50 1 105 231 77 201 2.41e-42 145
Msa1413650 AT1G20270.3 59.055 127 50 1 105 231 77 201 2.41e-42 145
Msa1413650 AT4G35820.1 45.833 192 78 4 105 288 89 262 4.84e-42 146
Msa1413650 AT4G33910.1 38.428 229 130 4 81 305 61 282 6.89e-42 147
Msa1413650 AT2G23096.1 34.466 206 129 3 107 309 71 273 7.94e-34 125
Msa1413650 AT4G25600.1 29.435 248 140 7 73 310 21 243 1.89e-22 95.1
Msa1413650 AT4G25600.4 29.703 202 114 5 112 310 3 179 1.33e-18 83.2
Msa1413650 AT4G25600.3 29.703 202 114 5 112 310 6 182 1.38e-18 83.6
Msa1413650 AT4G25600.2 29.703 202 114 5 112 310 15 191 1.74e-18 83.2

Find 98 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACAATGCTTATGTATTT+AGG 0.204792 tig0024042:+77551 Msa1413650:CDS
CTTGAAGTTGTTGTTGTAAA+TGG 0.219348 tig0024042:-76986 None:intergenic
CTTATTTCCACAACTTCTTT+AGG 0.222275 tig0024042:-79614 None:intergenic
GTCACAATCGGAATGATTAT+TGG 0.231053 tig0024042:+76828 Msa1413650:CDS
CGGATCGAATCGATTCAATT+TGG 0.233243 tig0024042:+76902 Msa1413650:CDS
ACGAGGAATGTGATTATCTT+AGG 0.235744 tig0024042:+77134 Msa1413650:CDS
AGTTGGTCTTAGTACTTAAT+TGG 0.245028 tig0024042:+79429 Msa1413650:three_prime_UTR
TCTCAAATTCCAATTGAAAA+TGG 0.255513 tig0024042:+77316 Msa1413650:CDS
TTGGATAAGCAGCTAAATTA+AGG 0.273419 tig0024042:+79500 Msa1413650:three_prime_UTR
AGCACAGCATTTCCTTTAAC+TGG 0.282461 tig0024042:-78895 None:intergenic
GGAGGATGCCCGGTACTTGC+TGG 0.290409 tig0024042:+79094 Msa1413650:CDS
GTCGTCGTTATTATAACCTC+TGG 0.331910 tig0024042:-77020 None:intergenic
CAATTTGGGATTACAATTGC+AGG 0.351451 tig0024042:+76917 Msa1413650:CDS
CGCATGATTTAGCTAGCTTT+CGG 0.353098 tig0024042:+79159 Msa1413650:exon
GGATCGAATCGATTCAATTT+GGG 0.361388 tig0024042:+76903 Msa1413650:CDS
TTTCTGATCAAGGCTTTGTT+TGG 0.366792 tig0024042:+79481 Msa1413650:three_prime_UTR
TTCAGCTCCATTTCTGATCA+AGG 0.388513 tig0024042:+79471 Msa1413650:three_prime_UTR
TTATGTGTGAAACCAGTTAA+AGG 0.389095 tig0024042:+78883 Msa1413650:CDS
AACAAAGCCTTGATCAGAAA+TGG 0.390787 tig0024042:-79478 None:intergenic
AGTGATGTCAGAACAAGTTC+TGG 0.417022 tig0024042:+77223 Msa1413650:CDS
GGAGACCAACTAAGGACTTC+AGG 0.418550 tig0024042:-77079 None:intergenic
CGAGGAATGTGATTATCTTA+GGG 0.422123 tig0024042:+77135 Msa1413650:CDS
TGCAGGTGCTCTCTCTCAAT+TGG 0.426323 tig0024042:+76934 Msa1413650:CDS
CTTCTTCTTCGCATGATGAT+CGG 0.439825 tig0024042:+76882 Msa1413650:CDS
CTTCTCACTTTCGTCACAAT+CGG 0.443635 tig0024042:+76816 Msa1413650:CDS
GATGGTTTGATCTATATCAT+TGG 0.448253 tig0024042:+79337 Msa1413650:three_prime_UTR
TCGTCGTTATTATAACCTCT+GGG 0.448332 tig0024042:-77019 None:intergenic
TGGTTTCACACATAACCCTT+TGG 0.454448 tig0024042:-78875 None:intergenic
CATCCTCCATGCACACTATC+CGG 0.458292 tig0024042:-79079 None:intergenic
CACTTCTCCCCAGCAAGTAC+CGG 0.463904 tig0024042:-79103 None:intergenic
GAGGATGCCCGGTACTTGCT+GGG 0.470338 tig0024042:+79095 Msa1413650:CDS
TATTTAGGTGACAATGTTGA+GGG 0.478530 tig0024042:+77566 Msa1413650:CDS
TTAGCTAGCTTTCGGTGGGT+AGG 0.486072 tig0024042:+79167 Msa1413650:three_prime_UTR
TTAAACCTGAAGTCCTTAGT+TGG 0.490970 tig0024042:+77074 Msa1413650:CDS
TCATGTGACCTTGTCATAGT+TGG 0.497920 tig0024042:+79412 Msa1413650:three_prime_UTR
TATATCCTTGCGATTGGTCT+CGG 0.499648 tig0024042:+79376 Msa1413650:three_prime_UTR
GTCGATGCAAACACAGGAAA+GGG 0.501803 tig0024042:+77193 Msa1413650:CDS
TAGCTAGCTTTCGGTGGGTA+GGG 0.504284 tig0024042:+79168 Msa1413650:three_prime_UTR
GAAGCACAAATCTTACGCCT+TGG 0.505360 tig0024042:+77046 Msa1413650:CDS
CCGGCGATGAAGATTGTGTT+CGG 0.507131 tig0024042:+76792 Msa1413650:CDS
AGGTGACAATGTTGAGGGAG+GGG 0.507663 tig0024042:+77571 Msa1413650:CDS
GAGAGCTTATGCAAGTCCTT+AGG 0.507911 tig0024042:+77338 Msa1413650:CDS
TTATGAAGTAAAATAATTCG+TGG 0.516165 tig0024042:-77100 None:intergenic
TAAAGATATATCCTTGCGAT+TGG 0.518385 tig0024042:+79370 Msa1413650:three_prime_UTR
TGATATTAATACACAATGGA+TGG 0.527767 tig0024042:+79319 Msa1413650:three_prime_UTR
TGGTATTATCAGGGACTGGA+TGG 0.528484 tig0024042:+79046 Msa1413650:intron
AGTTTCGATCAATAAAATAC+AGG 0.529356 tig0024042:+79288 Msa1413650:three_prime_UTR
TAGGTGACAATGTTGAGGGA+GGG 0.530460 tig0024042:+77570 Msa1413650:CDS
ATGATTTAGCTAGCTTTCGG+TGG 0.531891 tig0024042:+79162 Msa1413650:exon
ACTGGATGGACAGTCAGATC+CGG 0.531985 tig0024042:+79060 Msa1413650:CDS
ACTTTGAAGTGAAAATATGA+AGG 0.535155 tig0024042:-79216 None:intergenic
CGGTACTTGCTGGGGAGAAG+TGG 0.535804 tig0024042:+79104 Msa1413650:CDS
ATGGTTTGATCTATATCATT+GGG 0.540700 tig0024042:+79338 Msa1413650:three_prime_UTR
TAGTGTGCATGGAGGATGCC+CGG 0.552060 tig0024042:+79084 Msa1413650:CDS
TTAGGTGACAATGTTGAGGG+AGG 0.555757 tig0024042:+77569 Msa1413650:CDS
GTATTTAGGTGACAATGTTG+AGG 0.557593 tig0024042:+77565 Msa1413650:CDS
CTTTGAAGTGAAAATATGAA+GGG 0.561377 tig0024042:-79215 None:intergenic
AATATGAAGGGGAAAACCCC+TGG 0.566836 tig0024042:-79203 None:intergenic
GTGGTGGGAAATTAACCAAA+GGG 0.569757 tig0024042:+78860 Msa1413650:CDS
AATCGAATCGATGAGTCCTC+CGG 0.571781 tig0024042:+76773 Msa1413650:CDS
TAAGCATTGTAGCTATTCGC+TGG 0.573513 tig0024042:-77541 None:intergenic
TAATTCGTGGAGACCAACTA+AGG 0.573940 tig0024042:-77087 None:intergenic
CGTCGATGCAAACACAGGAA+AGG 0.581169 tig0024042:+77192 Msa1413650:CDS
ACTGATTCTTCTCATACCTA+AGG 0.583409 tig0024042:-77354 None:intergenic
TCAGATCCGGATAGTGTGCA+TGG 0.584101 tig0024042:+79073 Msa1413650:CDS
ACTTCTCCCCAGCAAGTACC+GGG 0.590424 tig0024042:-79102 None:intergenic
CCGAACACAATCTTCATCGC+CGG 0.591398 tig0024042:-76792 None:intergenic
GGTCGGCTACAAAGTGGATG+AGG 0.597597 tig0024042:+79125 Msa1413650:CDS
CTTCAGGTTTAACATATCCA+AGG 0.599046 tig0024042:-77063 None:intergenic
CAGTTCAATTTGAAGCGTGG+TGG 0.599421 tig0024042:+77518 Msa1413650:intron
AACTTCTTTAGGATAGCTTG+CGG 0.604033 tig0024042:-79603 None:intergenic
AATCACATTCCTCGTAGCTG+AGG 0.604915 tig0024042:-77126 None:intergenic
TGTTATTTCTATTTAAAACG+AGG 0.606376 tig0024042:+79561 Msa1413650:three_prime_UTR
TGATTTAGCTAGCTTTCGGT+GGG 0.607907 tig0024042:+79163 Msa1413650:exon
AAGCTATCCTAAAGAAGTTG+TGG 0.610343 tig0024042:+79607 Msa1413650:three_prime_UTR
TCAGATGAATGTAGCTGTGG+TGG 0.613560 tig0024042:+78844 Msa1413650:CDS
AGGATGCCCGGTACTTGCTG+GGG 0.623094 tig0024042:+79096 Msa1413650:CDS
AACACAATCTTCATCGCCGG+AGG 0.630657 tig0024042:-76789 None:intergenic
AAATACCGAGACCAATCGCA+AGG 0.632027 tig0024042:-79381 None:intergenic
AGAAGTGGTCGGCTACAAAG+TGG 0.632725 tig0024042:+79119 Msa1413650:CDS
GAGGAATGTGATTATCTTAG+GGG 0.634805 tig0024042:+77136 Msa1413650:CDS
TAACAGTTCAATTTGAAGCG+TGG 0.636945 tig0024042:+77515 Msa1413650:intron
TGTGGTGGGAAATTAACCAA+AGG 0.637747 tig0024042:+78859 Msa1413650:CDS
CAGATGAATGTAGCTGTGGT+GGG 0.637801 tig0024042:+78845 Msa1413650:CDS
GGTTCAGATGAATGTAGCTG+TGG 0.641033 tig0024042:+78841 Msa1413650:CDS
TTTGAAGTGAAAATATGAAG+GGG 0.641588 tig0024042:-79214 None:intergenic
ACTTGCTGGGGAGAAGTGGT+CGG 0.648554 tig0024042:+79108 Msa1413650:CDS
AGGGGAAACGCATTTCCCAT+CGG 0.656658 tig0024042:+77589 Msa1413650:CDS
ATAGTATAAATATTAAATAG+CGG 0.658559 tig0024042:+79263 Msa1413650:three_prime_UTR
TGTATGATATTAATACACAA+TGG 0.662040 tig0024042:+79315 Msa1413650:three_prime_UTR
ACTGTCGTCGATGCAAACAC+AGG 0.669566 tig0024042:+77187 Msa1413650:CDS
AAATGGAGCTGAAATCTCAA+CGG 0.670043 tig0024042:-79461 None:intergenic
CGTCGTTATTATAACCTCTG+GGG 0.670331 tig0024042:-77018 None:intergenic
TACTAAGACCAACTATGACA+AGG 0.677316 tig0024042:-79420 None:intergenic
TGGAGCTGAAATCTCAACGG+TGG 0.681243 tig0024042:-79458 None:intergenic
GATCCGGATAGTGTGCATGG+AGG 0.746880 tig0024042:+79076 Msa1413650:CDS
TCATAACTTCCTCAGCTACG+AGG 0.747861 tig0024042:+77117 Msa1413650:CDS
TCAAGATCACTACTCCCCAG+AGG 0.760039 tig0024042:+77004 Msa1413650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAAAAAAAAAAAAAA+GGG - tig0024042:77881-77900 None:intergenic 0.0%
!! ATAGTATAAATATTAAATAG+CGG + tig0024042:79263-79282 Msa1413650:three_prime_UTR 10.0%
!!! TGTTTTTTTACGAATATATA+CGG - tig0024042:77975-77994 None:intergenic 15.0%
!!! GATTTTTAACATAACAAAAA+GGG - tig0024042:78384-78403 None:intergenic 15.0%
!!! TTTTTGTTATGTTAAAAATC+CGG + tig0024042:78384-78403 Msa1413650:intron 15.0%
!! TTATGAAGTAAAATAATTCG+TGG - tig0024042:77103-77122 None:intergenic 20.0%
!!! GGATTTTTAACATAACAAAA+AGG - tig0024042:78385-78404 None:intergenic 20.0%
!!! AGATTGATTTAAATTGCATT+TGG - tig0024042:78637-78656 None:intergenic 20.0%
!!! ATTCTAGAAAACTTGTTATA+TGG + tig0024042:78997-79016 Msa1413650:intron 20.0%
!! TGTATGATATTAATACACAA+TGG + tig0024042:79315-79334 Msa1413650:three_prime_UTR 20.0%
!!! TGTTATTTCTATTTAAAACG+AGG + tig0024042:79561-79580 Msa1413650:three_prime_UTR 20.0%
!! TTTTCAATTGCATGTATCAT+AGG - tig0024042:77280-77299 None:intergenic 25.0%
! TCTCAAATTCCAATTGAAAA+TGG + tig0024042:77316-77335 Msa1413650:CDS 25.0%
! ATCAGAAAAATAGTCATGAT+GGG - tig0024042:77389-77408 None:intergenic 25.0%
! TATCAGAAAAATAGTCATGA+TGG - tig0024042:77390-77409 None:intergenic 25.0%
!! TCAAATGATAGTTTTCGAAA+AGG - tig0024042:77624-77643 None:intergenic 25.0%
! TATCTTCTGCTATAGATTAA+GGG - tig0024042:77650-77669 None:intergenic 25.0%
! ATATCTTCTGCTATAGATTA+AGG - tig0024042:77651-77670 None:intergenic 25.0%
! TTGAACTCATTATGAATGAA+AGG - tig0024042:77738-77757 None:intergenic 25.0%
! CATTCATAATGAGTTCAATT+TGG + tig0024042:77740-77759 Msa1413650:intron 25.0%
! AGTTCAATTTGGATTATTCT+GGG + tig0024042:77751-77770 Msa1413650:intron 25.0%
!! TTTTCAAAGTAGATAACAAG+TGG + tig0024042:77774-77793 Msa1413650:intron 25.0%
! ATTGCGAAGAATAAAAATTC+CGG - tig0024042:78330-78349 None:intergenic 25.0%
!! TTTTAACATAACAAAAAGGG+TGG - tig0024042:78381-78400 None:intergenic 25.0%
!! TTGTTATTAATGAAGTGGTT+TGG - tig0024042:78548-78567 None:intergenic 25.0%
!! TTTGGTTGTTATTAATGAAG+TGG - tig0024042:78553-78572 None:intergenic 25.0%
!! TTTGAAGTGAAAATATGAAG+GGG - tig0024042:79217-79236 None:intergenic 25.0%
!!! CTTTGAAGTGAAAATATGAA+GGG - tig0024042:79218-79237 None:intergenic 25.0%
!!! ACTTTGAAGTGAAAATATGA+AGG - tig0024042:79219-79238 None:intergenic 25.0%
! AGTTTCGATCAATAAAATAC+AGG + tig0024042:79288-79307 Msa1413650:three_prime_UTR 25.0%
! TGATATTAATACACAATGGA+TGG + tig0024042:79319-79338 Msa1413650:three_prime_UTR 25.0%
! ATGGTTTGATCTATATCATT+GGG + tig0024042:79338-79357 Msa1413650:three_prime_UTR 25.0%
! CTTGAAGTTGTTGTTGTAAA+TGG - tig0024042:76989-77008 None:intergenic 30.0%
! ATAAGCTCTCCATTTTCAAT+TGG - tig0024042:77328-77347 None:intergenic 30.0%
TCAGAAAAATAGTCATGATG+GGG - tig0024042:77388-77407 None:intergenic 30.0%
GCTACAATGCTTATGTATTT+AGG + tig0024042:77551-77570 Msa1413650:CDS 30.0%
TATTTAGGTGACAATGTTGA+GGG + tig0024042:77566-77585 Msa1413650:CDS 30.0%
AATGAAAGGAATGTGGAATA+AGG - tig0024042:77724-77743 None:intergenic 30.0%
CATTATGAATGAAAGGAATG+TGG - tig0024042:77731-77750 None:intergenic 30.0%
GAGTTCAATTTGGATTATTC+TGG + tig0024042:77750-77769 Msa1413650:intron 30.0%
AAAAAAAAAAGGGAGACACA+AGG - tig0024042:77871-77890 None:intergenic 30.0%
! ATGTATTTCGTTGTTTCATG+CGG - tig0024042:77930-77949 None:intergenic 30.0%
TGTTGTTGAGTTCTTGATTA+GGG - tig0024042:78021-78040 None:intergenic 30.0%
! TTGTTGTTGAGTTCTTGATT+AGG - tig0024042:78022-78041 None:intergenic 30.0%
!!! GGTAATGAGGTATTTTTTCT+GGG - tig0024042:78259-78278 None:intergenic 30.0%
!!! AGGTAATGAGGTATTTTTTC+TGG - tig0024042:78260-78279 None:intergenic 30.0%
! GTATGTTTTATGAATGGTAG+TGG - tig0024042:78304-78323 None:intergenic 30.0%
CATTCATAAAACATACAAGC+CGG + tig0024042:78308-78327 Msa1413650:intron 30.0%
CTAATAAGAAAATCCGGAAA+GGG - tig0024042:78463-78482 None:intergenic 30.0%
ACTAATAAGAAAATCCGGAA+AGG - tig0024042:78464-78483 None:intergenic 30.0%
GGGTAACTAATAAGAAAATC+CGG - tig0024042:78469-78488 None:intergenic 30.0%
TTCCTACTGATAAACAAGTT+TGG + tig0024042:78503-78522 Msa1413650:intron 30.0%
! ACACTTGCATTTTACACATT+GGG - tig0024042:78719-78738 None:intergenic 30.0%
! AACACTTGCATTTTACACAT+TGG - tig0024042:78720-78739 None:intergenic 30.0%
CTATTATCAAACACTCTAGT+TGG + tig0024042:78764-78783 Msa1413650:intron 30.0%
TTATGTGTGAAACCAGTTAA+AGG + tig0024042:78883-78902 Msa1413650:CDS 30.0%
TAGTATAACTTTGCCTTTGA+AGG + tig0024042:78933-78952 Msa1413650:intron 30.0%
AGATGAATGATTTCCTTCAA+AGG - tig0024042:78949-78968 None:intergenic 30.0%
TTTGAAGGAAATCATTCATC+TGG + tig0024042:78948-78967 Msa1413650:intron 30.0%
GATAGTAATTCTTATCTGCT+TGG + tig0024042:79026-79045 Msa1413650:intron 30.0%
TTATCTGCTTGGTATTATCA+GGG + tig0024042:79037-79056 Msa1413650:intron 30.0%
TAGGGTAAAAAAAATCACCA+GGG + tig0024042:79186-79205 Msa1413650:three_prime_UTR 30.0%
GATGGTTTGATCTATATCAT+TGG + tig0024042:79337-79356 Msa1413650:three_prime_UTR 30.0%
TAAAGATATATCCTTGCGAT+TGG + tig0024042:79370-79389 Msa1413650:three_prime_UTR 30.0%
!! AGTTGGTCTTAGTACTTAAT+TGG + tig0024042:79429-79448 Msa1413650:three_prime_UTR 30.0%
TTGGATAAGCAGCTAAATTA+AGG + tig0024042:79500-79519 Msa1413650:three_prime_UTR 30.0%
CTTATTTCCACAACTTCTTT+AGG - tig0024042:79617-79636 None:intergenic 30.0%
GTCACAATCGGAATGATTAT+TGG + tig0024042:76828-76847 Msa1413650:CDS 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG - tig0024042:76872-76891 None:intergenic 35.0%
! GGATCGAATCGATTCAATTT+GGG + tig0024042:76903-76922 Msa1413650:CDS 35.0%
! CAATTTGGGATTACAATTGC+AGG + tig0024042:76917-76936 Msa1413650:CDS 35.0%
TCGTCGTTATTATAACCTCT+GGG - tig0024042:77022-77041 None:intergenic 35.0%
! CTTCAGGTTTAACATATCCA+AGG - tig0024042:77066-77085 None:intergenic 35.0%
TTAAACCTGAAGTCCTTAGT+TGG + tig0024042:77074-77093 Msa1413650:CDS 35.0%
ACGAGGAATGTGATTATCTT+AGG + tig0024042:77134-77153 Msa1413650:CDS 35.0%
CGAGGAATGTGATTATCTTA+GGG + tig0024042:77135-77154 Msa1413650:CDS 35.0%
GAGGAATGTGATTATCTTAG+GGG + tig0024042:77136-77155 Msa1413650:CDS 35.0%
!!! CAGTTGAAATTTTGAGACGA+GGG - tig0024042:77171-77190 None:intergenic 35.0%
!!! ACAGTTGAAATTTTGAGACG+AGG - tig0024042:77172-77191 None:intergenic 35.0%
! ACTGATTCTTCTCATACCTA+AGG - tig0024042:77357-77376 None:intergenic 35.0%
AACTGTTAAGCAAGCAGAAA+GGG - tig0024042:77503-77522 None:intergenic 35.0%
! TAACAGTTCAATTTGAAGCG+TGG + tig0024042:77515-77534 Msa1413650:intron 35.0%
GTATTTAGGTGACAATGTTG+AGG + tig0024042:77565-77584 Msa1413650:CDS 35.0%
CACAAAAGATACATGCATCT+AGG - tig0024042:77682-77701 None:intergenic 35.0%
! GTATCTTTTGTGTTTTGTCC+CGG + tig0024042:77690-77709 Msa1413650:intron 35.0%
AAGGAATGTGGAATAAGGTT+TGG - tig0024042:77719-77738 None:intergenic 35.0%
! TGTATTTCGTTGTTTCATGC+GGG - tig0024042:77929-77948 None:intergenic 35.0%
! GTGTTGTAGTATCTGTTTTG+AGG - tig0024042:78061-78080 None:intergenic 35.0%
AGAACAACCTCAAAATGACA+AGG + tig0024042:78179-78198 Msa1413650:intron 35.0%
!!! GAGTTTTCCTTGTCATTTTG+AGG - tig0024042:78189-78208 None:intergenic 35.0%
!!! GATTGTCTTTTGAGGTAATG+AGG - tig0024042:78272-78291 None:intergenic 35.0%
! CGGCTTGTATGTTTTATGAA+TGG - tig0024042:78310-78329 None:intergenic 35.0%
AACAAAAAGGGTGGAAGATT+TGG - tig0024042:78372-78391 None:intergenic 35.0%
ATGCGGCTATTTGGATTATT+TGG - tig0024042:78424-78443 None:intergenic 35.0%
! TGTTTATCAGTAGGAAGTGT+TGG - tig0024042:78499-78518 None:intergenic 35.0%
! CACCAAACTTGTTTATCAGT+AGG - tig0024042:78508-78527 None:intergenic 35.0%
CTACTGATAAACAAGTTTGG+TGG + tig0024042:78506-78525 Msa1413650:intron 35.0%
!! AATTGCATTTGGTGTACGAT+GGG - tig0024042:78626-78645 None:intergenic 35.0%
!! AAATTGCATTTGGTGTACGA+TGG - tig0024042:78627-78646 None:intergenic 35.0%
CTTATCTGCTTGGTATTATC+AGG + tig0024042:79036-79055 Msa1413650:intron 35.0%
GTAGGGTAAAAAAAATCACC+AGG + tig0024042:79185-79204 Msa1413650:three_prime_UTR 35.0%
AGGGTAAAAAAAATCACCAG+GGG + tig0024042:79187-79206 Msa1413650:three_prime_UTR 35.0%
TACTAAGACCAACTATGACA+AGG - tig0024042:79423-79442 None:intergenic 35.0%
AAATGGAGCTGAAATCTCAA+CGG - tig0024042:79464-79483 None:intergenic 35.0%
AACAAAGCCTTGATCAGAAA+TGG - tig0024042:79481-79500 None:intergenic 35.0%
!! TTTCTGATCAAGGCTTTGTT+TGG + tig0024042:79481-79500 Msa1413650:three_prime_UTR 35.0%
!! AACTTCTTTAGGATAGCTTG+CGG - tig0024042:79606-79625 None:intergenic 35.0%
AAGCTATCCTAAAGAAGTTG+TGG + tig0024042:79607-79626 Msa1413650:three_prime_UTR 35.0%
CTTCTCACTTTCGTCACAAT+CGG + tig0024042:76816-76835 Msa1413650:CDS 40.0%
CTTCTTCTTCGCATGATGAT+CGG + tig0024042:76882-76901 Msa1413650:CDS 40.0%
! CGGATCGAATCGATTCAATT+TGG + tig0024042:76902-76921 Msa1413650:CDS 40.0%
CGTCGTTATTATAACCTCTG+GGG - tig0024042:77021-77040 None:intergenic 40.0%
GTCGTCGTTATTATAACCTC+TGG - tig0024042:77023-77042 None:intergenic 40.0%
TAATTCGTGGAGACCAACTA+AGG - tig0024042:77090-77109 None:intergenic 40.0%
!!! TGAAATTTTGAGACGAGGGA+GGG - tig0024042:77167-77186 None:intergenic 40.0%
!!! TTGAAATTTTGAGACGAGGG+AGG - tig0024042:77168-77187 None:intergenic 40.0%
AGTGATGTCAGAACAAGTTC+TGG + tig0024042:77223-77242 Msa1413650:CDS 40.0%
AAAGGGTTGCATTGCAATTG+AGG - tig0024042:77486-77505 None:intergenic 40.0%
GAACTGTTAAGCAAGCAGAA+AGG - tig0024042:77504-77523 None:intergenic 40.0%
! TAAGCATTGTAGCTATTCGC+TGG - tig0024042:77544-77563 None:intergenic 40.0%
AAAAGGAGAAGCTTACCGAT+GGG - tig0024042:77607-77626 None:intergenic 40.0%
TGGAATAAGGTTTGGATTCC+GGG - tig0024042:77711-77730 None:intergenic 40.0%
GTGGAATAAGGTTTGGATTC+CGG - tig0024042:77712-77731 None:intergenic 40.0%
!!! AGCTGTTTTGAGGTTCTATG+GGG - tig0024042:77998-78017 None:intergenic 40.0%
!!! GAGCTGTTTTGAGGTTCTAT+GGG - tig0024042:77999-78018 None:intergenic 40.0%
!! TTGATTAGGGAGCTGTTTTG+AGG - tig0024042:78008-78027 None:intergenic 40.0%
!! TGTTTCTGTCAGGTTTTGAC+AGG - tig0024042:78104-78123 None:intergenic 40.0%
!! GGTGTTTTTGTGTTTCTGTC+AGG - tig0024042:78114-78133 None:intergenic 40.0%
!!! AGGTATTTTTTCTGGGATGC+TGG - tig0024042:78252-78271 None:intergenic 40.0%
TACCTCAAAAGACAATCGCT+CGG + tig0024042:78275-78294 Msa1413650:intron 40.0%
!! AGATTTGGTGGAGGAGATTA+AGG - tig0024042:78357-78376 None:intergenic 40.0%
AAAAAGGGTGGAAGATTTGG+TGG - tig0024042:78369-78388 None:intergenic 40.0%
TTTGGAAAGCGAGAAATGAC+CGG - tig0024042:78406-78425 None:intergenic 40.0%
ATAAGAAAATCCGGAAAGGG+AGG - tig0024042:78460-78479 None:intergenic 40.0%
!! ATTGCATTTGGTGTACGATG+GGG - tig0024042:78625-78644 None:intergenic 40.0%
CAAGAAGGAATCTTGCGATT+AGG - tig0024042:78670-78689 None:intergenic 40.0%
CTAATCGCAAGATTCCTTCT+TGG + tig0024042:78668-78687 Msa1413650:intron 40.0%
GGTTATAGGCAAATCCAAGA+AGG - tig0024042:78685-78704 None:intergenic 40.0%
!!! GGACATTTTGACTCTACTCA+AGG + tig0024042:78785-78804 Msa1413650:intron 40.0%
GATAGTATGCAATGCGTTTC+AGG + tig0024042:78816-78835 Msa1413650:intron 40.0%
! TGTGGTGGGAAATTAACCAA+AGG + tig0024042:78859-78878 Msa1413650:CDS 40.0%
! GTGGTGGGAAATTAACCAAA+GGG + tig0024042:78860-78879 Msa1413650:CDS 40.0%
TGGTTTCACACATAACCCTT+TGG - tig0024042:78878-78897 None:intergenic 40.0%
AGCACAGCATTTCCTTTAAC+TGG - tig0024042:78898-78917 None:intergenic 40.0%
! AAGGAAATGCTGTGCTTTTC+TGG + tig0024042:78902-78921 Msa1413650:CDS 40.0%
!! CGCATGATTTAGCTAGCTTT+CGG + tig0024042:79159-79178 Msa1413650:exon 40.0%
!! ATGATTTAGCTAGCTTTCGG+TGG + tig0024042:79162-79181 Msa1413650:exon 40.0%
!! TGATTTAGCTAGCTTTCGGT+GGG + tig0024042:79163-79182 Msa1413650:exon 40.0%
!! TATATCCTTGCGATTGGTCT+CGG + tig0024042:79376-79395 Msa1413650:three_prime_UTR 40.0%
TCATGTGACCTTGTCATAGT+TGG + tig0024042:79412-79431 Msa1413650:three_prime_UTR 40.0%
TTCAGCTCCATTTCTGATCA+AGG + tig0024042:79471-79490 Msa1413650:three_prime_UTR 40.0%
AATCGAATCGATGAGTCCTC+CGG + tig0024042:76773-76792 Msa1413650:CDS 45.0%
GAAGCACAAATCTTACGCCT+TGG + tig0024042:77046-77065 Msa1413650:CDS 45.0%
TCATAACTTCCTCAGCTACG+AGG + tig0024042:77117-77136 Msa1413650:CDS 45.0%
AATCACATTCCTCGTAGCTG+AGG - tig0024042:77129-77148 None:intergenic 45.0%
GTCGATGCAAACACAGGAAA+GGG + tig0024042:77193-77212 Msa1413650:CDS 45.0%
GAGAGCTTATGCAAGTCCTT+AGG + tig0024042:77338-77357 Msa1413650:CDS 45.0%
! CAGTTCAATTTGAAGCGTGG+TGG + tig0024042:77518-77537 Msa1413650:intron 45.0%
TTAGGTGACAATGTTGAGGG+AGG + tig0024042:77569-77588 Msa1413650:CDS 45.0%
TAGGTGACAATGTTGAGGGA+GGG + tig0024042:77570-77589 Msa1413650:CDS 45.0%
GAAAAGGAGAAGCTTACCGA+TGG - tig0024042:77608-77627 None:intergenic 45.0%
ACATACGCGACGAAAAAGAG+AGG + tig0024042:77946-77965 Msa1413650:intron 45.0%
!!! GGAGCTGTTTTGAGGTTCTA+TGG - tig0024042:78000-78019 None:intergenic 45.0%
!!! ATTTTTTCTGGGATGCTGGC+AGG - tig0024042:78248-78267 None:intergenic 45.0%
ACCTCAAAAGACAATCGCTC+GGG + tig0024042:78276-78295 Msa1413650:intron 45.0%
!! TCAGTAGGAAGTGTTGGAGT+GGG - tig0024042:78493-78512 None:intergenic 45.0%
!! ATCAGTAGGAAGTGTTGGAG+TGG - tig0024042:78494-78513 None:intergenic 45.0%
CTTATTCCTGCATTGCGAGT+TGG - tig0024042:78585-78604 None:intergenic 45.0%
!! TTGCATTTGGTGTACGATGG+GGG - tig0024042:78624-78643 None:intergenic 45.0%
GGGCATGTTTCTCAGGTTAT+AGG - tig0024042:78699-78718 None:intergenic 45.0%
ACACATTGGGCATGTTTCTC+AGG - tig0024042:78706-78725 None:intergenic 45.0%
GGTTCAGATGAATGTAGCTG+TGG + tig0024042:78841-78860 Msa1413650:CDS 45.0%
TCAGATGAATGTAGCTGTGG+TGG + tig0024042:78844-78863 Msa1413650:CDS 45.0%
CAGATGAATGTAGCTGTGGT+GGG + tig0024042:78845-78864 Msa1413650:CDS 45.0%
!!! TGCTGTGCTTTTCTGGAGTA+TGG + tig0024042:78909-78928 Msa1413650:CDS 45.0%
TGCTTGGTATTATCAGGGAC+TGG + tig0024042:79042-79061 Msa1413650:intron 45.0%
TGGTATTATCAGGGACTGGA+TGG + tig0024042:79046-79065 Msa1413650:intron 45.0%
AATATGAAGGGGAAAACCCC+TGG - tig0024042:79206-79225 None:intergenic 45.0%
AAATACCGAGACCAATCGCA+AGG - tig0024042:79384-79403 None:intergenic 45.0%
!! GATTAAAAAAAAAAAAAAAA+AGG - tig0024042:77882-77901 None:intergenic 5.0%
AACACAATCTTCATCGCCGG+AGG - tig0024042:76792-76811 None:intergenic 50.0%
CCGAACACAATCTTCATCGC+CGG - tig0024042:76795-76814 None:intergenic 50.0%
CCGGCGATGAAGATTGTGTT+CGG + tig0024042:76792-76811 Msa1413650:CDS 50.0%
TGCAGGTGCTCTCTCTCAAT+TGG + tig0024042:76934-76953 Msa1413650:CDS 50.0%
TCAAGATCACTACTCCCCAG+AGG + tig0024042:77004-77023 Msa1413650:CDS 50.0%
GGAGACCAACTAAGGACTTC+AGG - tig0024042:77082-77101 None:intergenic 50.0%
ACTGTCGTCGATGCAAACAC+AGG + tig0024042:77187-77206 Msa1413650:CDS 50.0%
CGTCGATGCAAACACAGGAA+AGG + tig0024042:77192-77211 Msa1413650:CDS 50.0%
AGGTGACAATGTTGAGGGAG+GGG + tig0024042:77571-77590 Msa1413650:CDS 50.0%
AGGGGAAACGCATTTCCCAT+CGG + tig0024042:77589-77608 Msa1413650:CDS 50.0%
TGACAGGTGCAGATAGCAGT+AGG - tig0024042:78088-78107 None:intergenic 50.0%
! GCTTTTGAGCTGTTGCGCTT+GGG - tig0024042:78143-78162 None:intergenic 50.0%
! TGCTTTTGAGCTGTTGCGCT+TGG - tig0024042:78144-78163 None:intergenic 50.0%
! ACCCGAGCGATTGTCTTTTG+AGG - tig0024042:78280-78299 None:intergenic 50.0%
!! AAGGGTGGAAGATTTGGTGG+AGG - tig0024042:78366-78385 None:intergenic 50.0%
TAGAGTGGCATGCGGCTATT+TGG - tig0024042:78433-78452 None:intergenic 50.0%
ACTCTATCCACCTCCCTTTC+CGG + tig0024042:78447-78466 Msa1413650:intron 50.0%
AGAAAATCCGGAAAGGGAGG+TGG - tig0024042:78457-78476 None:intergenic 50.0%
!! AGTAGGAAGTGTTGGAGTGG+GGG - tig0024042:78491-78510 None:intergenic 50.0%
!! CAGTAGGAAGTGTTGGAGTG+GGG - tig0024042:78492-78511 None:intergenic 50.0%
GTATGCAATGCGTTTCAGGC+TGG + tig0024042:78820-78839 Msa1413650:intron 50.0%
ACTGGATGGACAGTCAGATC+CGG + tig0024042:79060-79079 Msa1413650:CDS 50.0%
TCAGATCCGGATAGTGTGCA+TGG + tig0024042:79073-79092 Msa1413650:CDS 50.0%
CATCCTCCATGCACACTATC+CGG - tig0024042:79082-79101 None:intergenic 50.0%
AGAAGTGGTCGGCTACAAAG+TGG + tig0024042:79119-79138 Msa1413650:CDS 50.0%
!! TTAGCTAGCTTTCGGTGGGT+AGG + tig0024042:79167-79186 Msa1413650:three_prime_UTR 50.0%
!! TAGCTAGCTTTCGGTGGGTA+GGG + tig0024042:79168-79187 Msa1413650:three_prime_UTR 50.0%
TGGAGCTGAAATCTCAACGG+TGG - tig0024042:79461-79480 None:intergenic 50.0%
GAGGTGGATAGAGTGGCATG+CGG - tig0024042:78441-78460 None:intergenic 55.0%
GGAAAGGGAGGTGGATAGAG+TGG - tig0024042:78448-78467 None:intergenic 55.0%
!! TAGGAAGTGTTGGAGTGGGG+GGG - tig0024042:78489-78508 None:intergenic 55.0%
!! GTAGGAAGTGTTGGAGTGGG+GGG - tig0024042:78490-78509 None:intergenic 55.0%
! GCGAGTTGGCGTGGAATGTT+TGG - tig0024042:78571-78590 None:intergenic 55.0%
GATCCGGATAGTGTGCATGG+AGG + tig0024042:79076-79095 Msa1413650:CDS 55.0%
TAGTGTGCATGGAGGATGCC+CGG + tig0024042:79084-79103 Msa1413650:CDS 55.0%
ACTTCTCCCCAGCAAGTACC+GGG - tig0024042:79105-79124 None:intergenic 55.0%
CACTTCTCCCCAGCAAGTAC+CGG - tig0024042:79106-79125 None:intergenic 55.0%
! ACTTGCTGGGGAGAAGTGGT+CGG + tig0024042:79108-79127 Msa1413650:CDS 55.0%
GGTCGGCTACAAAGTGGATG+AGG + tig0024042:79125-79144 Msa1413650:CDS 55.0%
! GCTGTTGCGCTTGGGTTGCT+GGG - tig0024042:78135-78154 None:intergenic 60.0%
! AGCTGTTGCGCTTGGGTTGC+TGG - tig0024042:78136-78155 None:intergenic 60.0%
TCCACGCCAACTCGCAATGC+AGG + tig0024042:78576-78595 Msa1413650:intron 60.0%
! TCCTGCATTGCGAGTTGGCG+TGG - tig0024042:78580-78599 None:intergenic 60.0%
GAGGATGCCCGGTACTTGCT+GGG + tig0024042:79095-79114 Msa1413650:CDS 60.0%
! AGGATGCCCGGTACTTGCTG+GGG + tig0024042:79096-79115 Msa1413650:CDS 60.0%
! CGGTACTTGCTGGGGAGAAG+TGG + tig0024042:79104-79123 Msa1413650:CDS 60.0%
! TGCTGGCAGGTGCTGTGGGT+TGG - tig0024042:78235-78254 None:intergenic 65.0%
! GGGATGCTGGCAGGTGCTGT+GGG - tig0024042:78239-78258 None:intergenic 65.0%
! TGGGATGCTGGCAGGTGCTG+TGG - tig0024042:78240-78259 None:intergenic 65.0%
GGAGGATGCCCGGTACTTGC+TGG + tig0024042:79094-79113 Msa1413650:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0024042 gene 76770 79695 76770 ID=Msa1413650;Name=Msa1413650
tig0024042 mRNA 76770 79695 76770 ID=Msa1413650-mRNA-1;Parent=Msa1413650;Name=Msa1413650-mRNA-1;_AED=0.09;_eAED=0.14;_QI=0|1|0.75|1|1|1|4|530|310
tig0024042 exon 76770 77411 76770 ID=Msa1413650-mRNA-1:exon:21276;Parent=Msa1413650-mRNA-1
tig0024042 exon 77521 77610 77521 ID=Msa1413650-mRNA-1:exon:21277;Parent=Msa1413650-mRNA-1
tig0024042 exon 78838 78930 78838 ID=Msa1413650-mRNA-1:exon:21278;Parent=Msa1413650-mRNA-1
tig0024042 exon 79058 79695 79058 ID=Msa1413650-mRNA-1:exon:21279;Parent=Msa1413650-mRNA-1
tig0024042 CDS 76770 77411 76770 ID=Msa1413650-mRNA-1:cds;Parent=Msa1413650-mRNA-1
tig0024042 CDS 77521 77610 77521 ID=Msa1413650-mRNA-1:cds;Parent=Msa1413650-mRNA-1
tig0024042 CDS 78838 78930 78838 ID=Msa1413650-mRNA-1:cds;Parent=Msa1413650-mRNA-1
tig0024042 CDS 79058 79165 79058 ID=Msa1413650-mRNA-1:cds;Parent=Msa1413650-mRNA-1
tig0024042 three_prime_UTR 79166 79695 79166 ID=Msa1413650-mRNA-1:three_prime_utr;Parent=Msa1413650-mRNA-1
Gene Sequence

>Msa1413650

ATGAATCGAATCGATGAGTCCTCCGGCGATGAAGATTGTGTTCGGACTTCTCACTTTCGTCACAATCGGAATGATTATTGGTATGTAATCTTTATTTATCCTTCTTCTTCTTCTTCTTCTTCGCATGATGATCGGATCGAATCGATTCAATTTGGGATTACAATTGCAGGTGCTCTCTCTCAATTGGCATTCATACGCAGATTAGAACTAGAAGAACCATTTACAACAACAACTTCAAGATCACTACTCCCCAGAGGTTATAATAACGACGACAAAGAAGCACAAATCTTACGCCTTGGATATGTTAAACCTGAAGTCCTTAGTTGGTCTCCACGAATTATTTTACTTCATAACTTCCTCAGCTACGAGGAATGTGATTATCTTAGGGGAGTTGCCCTCCCTCGTCTCAAAATTTCAACTGTCGTCGATGCAAACACAGGAAAGGGAATCAAGAGTGATGTCAGAACAAGTTCTGGTATGTTTCTGAATCATGAAGAGAGAAAATATCCTATGATACATGCAATTGAAAAAAGAATTTCTGTCTACTCTCAAATTCCAATTGAAAATGGAGAGCTTATGCAAGTCCTTAGGTATGAGAAGAATCAGTATTACAGACCCCATCATGACTATTTTTCTGATACTTTCAATTTGAAGCGTGGTGGCCAGCGAATAGCTACAATGCTTATGTATTTAGGTGACAATGTTGAGGGAGGGGAAACGCATTTCCCATCGGCTGGTTCAGATGAATGTAGCTGTGGTGGGAAATTAACCAAAGGGTTATGTGTGAAACCAGTTAAAGGAAATGCTGTGCTTTTCTGGAGTATGGGACTGGATGGACAGTCAGATCCGGATAGTGTGCATGGAGGATGCCCGGTACTTGCTGGGGAGAAGTGGTCGGCTACAAAGTGGATGAGGCAATCAGTTCACGCATGA
Protein sequence

>Msa1413650

MNRIDESSGDEDCVRTSHFRHNRNDYWYVIFIYPSSSSSSSHDDRIESIQFGITIAGALSQLAFIRRLELEEPFTTTTSRSLLPRGYNNDDKEAQILRLGYVKPEVLSWSPRIILLHNFLSYEECDYLRGVALPRLKISTVVDANTGKGIKSDVRTSSGMFLNHEERKYPMIHAIEKRISVYSQIPIENGELMQVLRYEKNQYYRPHHDYFSDTFNLKRGGQRIATMLMYLGDNVEGGETHFPSAGSDECSCGGKLTKGLCVKPVKGNAVLFWSMGLDGQSDPDSVHGGCPVLAGEKWSATKWMRQSVHA*