AlfalfaGEDB | Msa1415370

M. sativa cultivar zhongmu-4 / Msa1415370

KEGG: -
GO: -
PFAM: PF07798.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415370	sp\|Q9GZT6\|CC90B_HUMAN	27.919	197	124	2	42	220	56	252	4.21e-20	88.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415370	B7FKU2	97.321	224	4	1	1	222	1	224	3.03e-155	438

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0387010	Msa1415370	0.829880	4.018259e-55	-8.615850e-47
Msa0523950	Msa1415370	0.875879	2.174804e-68	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415370	MtrunA17_Chr3g0145131	96.460	226	4	1	1	222	1	226	7.32e-159	438
Msa1415370	MtrunA17_Chr8g0372241	74.877	203	49	1	22	222	39	241	9.93e-109	312
Msa1415370	MtrunA17_Chr5g0405011	69.907	216	57	2	7	222	26	233	1.26e-105	304

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415370	AT2G16460.1	72.174	230	56	2	1	222	1	230	5.39e-114	325
Msa1415370	AT3G51090.1	73.958	192	50	0	31	222	107	298	8.42e-101	294
Msa1415370	AT3G51090.2	71.242	153	44	0	31	183	107	259	2.42e-72	221
Msa1415370	AT2G16460.2	66.071	168	49	1	1	160	1	168	7.32e-71	214
Msa1415370	AT2G35090.1	27.841	176	81	4	47	222	28	157	1.01e-13	67.0
Msa1415370	AT2G35070.2	27.711	166	74	3	47	212	28	147	3.17e-12	62.8
Msa1415370	AT2G35070.1	27.711	166	74	3	47	212	28	147	3.17e-12	62.8

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Find 65 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGCGGAGGCGATAACT+TGG	0.423397	tig0024203:-47256	Msa1415370:CDS
AACTGCAGGTCTTGTACCAA+TGG	0.554731	tig0024203:+44296	None:intergenic
AACTTCATGTGTAGTATCCC+CGG	0.524399	tig0024203:-44231	Msa1415370:three_prime_UTR
AAGTTTGTTTGTCAGATTGG+TGG	0.471335	tig0024203:+44614	None:intergenic
AATGTTACAAATGAAACTGC+AGG	0.549552	tig0024203:+44282	None:intergenic
AATTCTATCTACTTTCTGAT+TGG	0.391161	tig0024203:+44155	None:intergenic
AATTGATTGTATTTGGTTGT+TGG	0.449835	tig0024203:-44190	Msa1415370:three_prime_UTR
ACAAGCGGAGGCGATAACTT+GGG	0.608734	tig0024203:-47255	Msa1415370:CDS
ACAATTCACCTTTGATTACA+TGG	0.433146	tig0024203:+44382	None:intergenic
AGAAAGGAATAAAGAAGCGA+CGG	0.605280	tig0024203:+47501	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTGATCACACTCTTTC+TGG	-	tig0024203:44751-44770	Msa1415370:intron	30.0%
TACTGTATCTATCAAACAGA+AGG	-	tig0024203:44891-44910	Msa1415370:intron	30.0%
CTATATGACATCTCAAAACT+CGG	+	tig0024203:44938-44957	None:intergenic	30.0%
ACAAGACGTTGAAAAAGAAA+TGG	-	tig0024203:45033-45052	Msa1415370:intron	30.0%
CAAGACGTTGAAAAAGAAAT+GGG	-	tig0024203:45034-45053	Msa1415370:intron	30.0%
AAGAACTTAGTAAACTGCAA+AGG	-	tig0024203:45107-45126	Msa1415370:intron	30.0%
ATTGCTGCATTATATATGCA+TGG	-	tig0024203:45139-45158	Msa1415370:intron	30.0%
ACGTGATATTGTGATGAAAA+AGG	+	tig0024203:45240-45259	None:intergenic	30.0%
TCATCACAATATCACGTATA+TGG	-	tig0024203:45243-45262	Msa1415370:intron	30.0%
TGACAATTATTGGAAGTTCA+TGG	-	tig0024203:45415-45434	Msa1415370:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024203	gene	44036	47656	44036	ID=Msa1415370;Name=Msa1415370
tig0024203	mRNA	44036	47656	44036	ID=Msa1415370-mRNA-1;Parent=Msa1415370;Name=Msa1415370-mRNA-1;_AED=0.06;_eAED=0.06;_QI=84\|1\|1\|1\|1\|1\|6\|373\|222
tig0024203	exon	44036	44528	44036	ID=Msa1415370-mRNA-1:exon:22369;Parent=Msa1415370-mRNA-1
tig0024203	exon	44608	44674	44608	ID=Msa1415370-mRNA-1:exon:22368;Parent=Msa1415370-mRNA-1
tig0024203	exon	44792	44848	44792	ID=Msa1415370-mRNA-1:exon:22367;Parent=Msa1415370-mRNA-1
tig0024203	exon	44965	45041	44965	ID=Msa1415370-mRNA-1:exon:22366;Parent=Msa1415370-mRNA-1
tig0024203	exon	47121	47312	47121	ID=Msa1415370-mRNA-1:exon:22365;Parent=Msa1415370-mRNA-1
tig0024203	exon	47417	47656	47417	ID=Msa1415370-mRNA-1:exon:22364;Parent=Msa1415370-mRNA-1
tig0024203	five_prime_UTR	47573	47656	47573	ID=Msa1415370-mRNA-1:five_prime_utr;Parent=Msa1415370-mRNA-1
tig0024203	CDS	47417	47572	47417	ID=Msa1415370-mRNA-1:cds;Parent=Msa1415370-mRNA-1
tig0024203	CDS	47121	47312	47121	ID=Msa1415370-mRNA-1:cds;Parent=Msa1415370-mRNA-1
tig0024203	CDS	44965	45041	44965	ID=Msa1415370-mRNA-1:cds;Parent=Msa1415370-mRNA-1
tig0024203	CDS	44792	44848	44792	ID=Msa1415370-mRNA-1:cds;Parent=Msa1415370-mRNA-1
tig0024203	CDS	44608	44674	44608	ID=Msa1415370-mRNA-1:cds;Parent=Msa1415370-mRNA-1
tig0024203	CDS	44409	44528	44409	ID=Msa1415370-mRNA-1:cds;Parent=Msa1415370-mRNA-1
tig0024203	three_prime_UTR	44036	44408	44036	ID=Msa1415370-mRNA-1:three_prime_utr;Parent=Msa1415370-mRNA-1

Gene Sequence

>Msa1415370

ATGGCGTTGTACAAACGTGCTTTTGATTTGGGTTCAAAATCTCGCTTCACCGTCGCTTCTTTATTCCTTTCTTCTACTTCTACTAGCACCAGGCATGTTTCACAGGCCGTCGATTCCAACGTTAAACGCGCATTCCTCGTCGATACACTTGCACTCGTACGGAATTTAGAGACGAACGGGCTTCCTCCAAAACAAGCGGAGGCGATAACTTGGGCAATCACTGAAGTTCTTAATGACAGCTTGGAAAATGTTGCTCAATCTTTCGTTACTAAATCCGATATGCAAAAATCTGAGATGATGCAAGAATCTAATCTCTCTAAGTTCAAATCTGAAGTCAAAACTTCTCAGGAGCATCATTTTTCTTTGTTGCAACGCGAGACTGAAAAGCTTCGTAATGACATAGATAAGATGAGGACTGAATTGAGGTATGAGATTGACAAAGTCACTGCTGGGCAACGATTGGACTTGAATCTTGAAAGAAGACGCATTCAGGATGAGCTTGCTACTCAAAATACTGAAACCACCAATCTGACAAACAAACTTGATCGGGAAATTCATGCATTGAGAGCCCAGTTAGAAGCTGCAAAGTATGATGTGATCAAATACTGCATAGGTACCCTTATCTCCATATCTGCAGTGGGTCTCGCCGTGATCCGCATCATATTATAG

Protein sequence

>Msa1415370

MALYKRAFDLGSKSRFTVASLFLSSTSTSTRHVSQAVDSNVKRAFLVDTLALVRNLETNGLPPKQAEAITWAITEVLNDSLENVAQSFVTKSDMQKSEMMQESNLSKFKSEVKTSQEHHFSLLQRETEKLRNDIDKMRTELRYEIDKVTAGQRLDLNLERRRIQDELATQNTETTNLTNKLDREIHALRAQLEAAKYDVIKYCIGTLISISAVGLAVIRIIL*