AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1418670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418670 MtrunA17_Chr4g0058091 97.701 174 4 0 1 174 1 174 3.82e-117 341
Msa1418670 MtrunA17_Chr4g0001841 78.621 145 7 1 30 174 20 140 7.56e-71 215
Msa1418670 MtrunA17_Chr3g0116561 67.857 168 54 0 7 174 2 169 2.14e-67 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418670 AT5G50230.1 67.066 167 55 0 8 174 3 169 2.80e-73 229

Find 27 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGAATTGATCACTAAATT+AGG 0.120625 tig0024623:-76680 None:intergenic
ACCATTGTTGTTGTTGTGTT+TGG 0.308418 tig0024623:-76705 None:intergenic
GATTGTATTATACCTCATTT+AGG 0.316444 tig0024623:-78029 None:intergenic
CAGAATTGATCACTAAATTA+GGG 0.329052 tig0024623:-76679 None:intergenic
CTCTCAATTAAAGACAGATT+TGG 0.339284 tig0024623:+77852 Msa1418670:CDS
TCAATCGCAACTTGTTCTTT+CGG 0.343457 tig0024623:-76741 None:intergenic
ACAACAACAATGGTGAAAAC+TGG 0.379287 tig0024623:+76714 Msa1418670:exon
TTCAGAACTAAAAGCACAAC+TGG 0.383721 tig0024623:+77915 Msa1418670:CDS
GAAAGAGAAAGCAGAGAACT+TGG 0.396574 tig0024623:+77372 Msa1418670:CDS
TTCTAGACCCATCGTTTCTC+AGG 0.404660 tig0024623:+76845 Msa1418670:CDS
CTAGAAAGTGCGAGAAAAGC+AGG 0.474966 tig0024623:-76828 None:intergenic
AGACTAGAGAGACCGAAAAT+AGG 0.486000 tig0024623:-76623 None:intergenic
GAAAGCAGAGAACTTGGAAG+TGG 0.488324 tig0024623:+77378 Msa1418670:CDS
AAACGCCATCTTCTCGAAGA+AGG 0.507490 tig0024623:+76795 Msa1418670:CDS
GAGCGCCTTCTTCGAGAAGA+TGG 0.521377 tig0024623:-76800 None:intergenic
TACTTCCACAACAAGTTGCT+CGG 0.529307 tig0024623:-77437 None:intergenic
AGAAGGAGTTGACAGAATTG+AGG 0.554456 tig0024623:+77521 Msa1418670:CDS
TGCAATTTCTGATATGCAGA+AGG 0.560064 tig0024623:+77504 Msa1418670:CDS
CTTGATGTTAGAAAAGATGA+AGG 0.562481 tig0024623:+77996 Msa1418670:CDS
GTTATCCGAGCAACTTGTTG+TGG 0.566280 tig0024623:+77432 Msa1418670:CDS
GGATGCTGAACGCCTAAATG+AGG 0.592570 tig0024623:+78017 Msa1418670:CDS
AATGCTATAAAGCTCAATCT+CGG 0.592904 tig0024623:+77410 Msa1418670:CDS
ATGATCTACTTATTTGTACG+TGG 0.604095 tig0024623:+76598 Msa1418670:five_prime_UTR
TGATCTACTTATTTGTACGT+GGG 0.620905 tig0024623:+76599 Msa1418670:five_prime_UTR
ACCAAACACAACAACAACAA+TGG 0.632413 tig0024623:+76704 Msa1418670:exon
GGTACGTACCTGAGAAACGA+TGG 0.636424 tig0024623:-76853 None:intergenic
GTACGTACCTGAGAAACGAT+GGG 0.661026 tig0024623:-76852 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACAATTTTCATTTTTTTT+AGG + tig0024623:76883-76902 Msa1418670:intron 10.0%
!!! AACAATTTTCATTTTTTTTA+GGG + tig0024623:76884-76903 Msa1418670:intron 10.0%
!!! TAACAATTTTCATTTTTTTT+AGG + tig0024623:76883-76902 Msa1418670:intron 10.0%
!!! AACAATTTTCATTTTTTTTA+GGG + tig0024623:76884-76903 Msa1418670:intron 10.0%
!! AATGAAAATTGTTAATGAAA+GGG - tig0024623:76877-76896 None:intergenic 15.0%
!! AAATGAAAATTGTTAATGAA+AGG - tig0024623:76878-76897 None:intergenic 15.0%
!! AGGAAACAATAAAAAAAAAA+TGG - tig0024623:76944-76963 None:intergenic 15.0%
!! AATGAAAATTGTTAATGAAA+GGG - tig0024623:76877-76896 None:intergenic 15.0%
!! AAATGAAAATTGTTAATGAA+AGG - tig0024623:76878-76897 None:intergenic 15.0%
!! AGGAAACAATAAAAAAAAAA+TGG - tig0024623:76944-76963 None:intergenic 15.0%
!!! TTTTATTGTTTCCTTTATTG+TGG + tig0024623:76950-76969 Msa1418670:intron 20.0%
!! ATGATTGAACTAATTTCTTA+CGG - tig0024623:76978-76997 None:intergenic 20.0%
!!! TTTTATTGTTTCCTTTATTG+TGG + tig0024623:76950-76969 Msa1418670:intron 20.0%
!! ATGATTGAACTAATTTCTTA+CGG - tig0024623:76978-76997 None:intergenic 20.0%
! CAGAATTGATCACTAAATTA+GGG - tig0024623:76682-76701 None:intergenic 25.0%
! TCAGAATTGATCACTAAATT+AGG - tig0024623:76683-76702 None:intergenic 25.0%
! TAATTCAATGTGATCACATA+TGG - tig0024623:77109-77128 None:intergenic 25.0%
! TAACTTTGCACAGTTAAAAT+AGG - tig0024623:77202-77221 None:intergenic 25.0%
!!! TTTTAACTGTGCAAAGTTAT+TGG + tig0024623:77203-77222 Msa1418670:intron 25.0%
! TGATGTATTTATGTTGTTGA+AGG + tig0024623:77339-77358 Msa1418670:intron 25.0%
! GATGTATTTATGTTGTTGAA+GGG + tig0024623:77340-77359 Msa1418670:intron 25.0%
! AAATTAATGTTGCAAAATGC+AGG + tig0024623:77821-77840 Msa1418670:intron 25.0%
! AATTAATGTTGCAAAATGCA+GGG + tig0024623:77822-77841 Msa1418670:intron 25.0%
! CAGAATTGATCACTAAATTA+GGG - tig0024623:76682-76701 None:intergenic 25.0%
! TCAGAATTGATCACTAAATT+AGG - tig0024623:76683-76702 None:intergenic 25.0%
! TAATTCAATGTGATCACATA+TGG - tig0024623:77109-77128 None:intergenic 25.0%
! TAACTTTGCACAGTTAAAAT+AGG - tig0024623:77202-77221 None:intergenic 25.0%
!!! TTTTAACTGTGCAAAGTTAT+TGG + tig0024623:77203-77222 Msa1418670:intron 25.0%
! TGATGTATTTATGTTGTTGA+AGG + tig0024623:77339-77358 Msa1418670:intron 25.0%
! GATGTATTTATGTTGTTGAA+GGG + tig0024623:77340-77359 Msa1418670:intron 25.0%
! AAATTAATGTTGCAAAATGC+AGG + tig0024623:77821-77840 Msa1418670:intron 25.0%
! AATTAATGTTGCAAAATGCA+GGG + tig0024623:77822-77841 Msa1418670:intron 25.0%
ATGATCTACTTATTTGTACG+TGG + tig0024623:76598-76617 Msa1418670:five_prime_UTR 30.0%
TGATCTACTTATTTGTACGT+GGG + tig0024623:76599-76618 Msa1418670:five_prime_UTR 30.0%
!! TTCTGATTCGAATGATTTTC+AGG - tig0024623:76652-76671 None:intergenic 30.0%
! ACTACTAAGCACTATTCTTT+TGG - tig0024623:77006-77025 None:intergenic 30.0%
AATGCTATAAAGCTCAATCT+CGG + tig0024623:77410-77429 Msa1418670:CDS 30.0%
! TGCATTTTCTTTCTCTTGAA+GGG - tig0024623:77488-77507 None:intergenic 30.0%
! TTGCATTTTCTTTCTCTTGA+AGG - tig0024623:77489-77508 None:intergenic 30.0%
! TAGCTGCTATTTAACAACAT+TGG - tig0024623:77574-77593 None:intergenic 30.0%
CAAATTCTGCTATGGTATAA+CGG - tig0024623:77602-77621 None:intergenic 30.0%
AGTGTATCTTGAAACTATAG+CGG - tig0024623:77631-77650 None:intergenic 30.0%
TACAAAATGTTGTCCAATAG+CGG + tig0024623:77657-77676 Msa1418670:intron 30.0%
ACTAAAAAAAATGTTGGTCG+CGG - tig0024623:77776-77795 None:intergenic 30.0%
TCGCAGACTAAAAAAAATGT+TGG - tig0024623:77782-77801 None:intergenic 30.0%
CTCTCAATTAAAGACAGATT+TGG + tig0024623:77852-77871 Msa1418670:CDS 30.0%
! TCATCTTTTCTAACATCAAG+CGG - tig0024623:77996-78015 None:intergenic 30.0%
CTTGATGTTAGAAAAGATGA+AGG + tig0024623:77996-78015 Msa1418670:CDS 30.0%
ATGATCTACTTATTTGTACG+TGG + tig0024623:76598-76617 Msa1418670:five_prime_UTR 30.0%
TGATCTACTTATTTGTACGT+GGG + tig0024623:76599-76618 Msa1418670:five_prime_UTR 30.0%
!! TTCTGATTCGAATGATTTTC+AGG - tig0024623:76652-76671 None:intergenic 30.0%
! ACTACTAAGCACTATTCTTT+TGG - tig0024623:77006-77025 None:intergenic 30.0%
AATGCTATAAAGCTCAATCT+CGG + tig0024623:77410-77429 Msa1418670:CDS 30.0%
! TGCATTTTCTTTCTCTTGAA+GGG - tig0024623:77488-77507 None:intergenic 30.0%
! TTGCATTTTCTTTCTCTTGA+AGG - tig0024623:77489-77508 None:intergenic 30.0%
! TAGCTGCTATTTAACAACAT+TGG - tig0024623:77574-77593 None:intergenic 30.0%
CAAATTCTGCTATGGTATAA+CGG - tig0024623:77602-77621 None:intergenic 30.0%
AGTGTATCTTGAAACTATAG+CGG - tig0024623:77631-77650 None:intergenic 30.0%
TACAAAATGTTGTCCAATAG+CGG + tig0024623:77657-77676 Msa1418670:intron 30.0%
ACTAAAAAAAATGTTGGTCG+CGG - tig0024623:77776-77795 None:intergenic 30.0%
TCGCAGACTAAAAAAAATGT+TGG - tig0024623:77782-77801 None:intergenic 30.0%
CTCTCAATTAAAGACAGATT+TGG + tig0024623:77852-77871 Msa1418670:CDS 30.0%
! TCATCTTTTCTAACATCAAG+CGG - tig0024623:77996-78015 None:intergenic 30.0%
CTTGATGTTAGAAAAGATGA+AGG + tig0024623:77996-78015 Msa1418670:CDS 30.0%
!! TGATTCGAATGATTTTCAGG+TGG - tig0024623:76649-76668 None:intergenic 35.0%
ACCAAACACAACAACAACAA+TGG + tig0024623:76704-76723 Msa1418670:exon 35.0%
! ACCATTGTTGTTGTTGTGTT+TGG - tig0024623:76708-76727 None:intergenic 35.0%
ACAACAACAATGGTGAAAAC+TGG + tig0024623:76714-76733 Msa1418670:exon 35.0%
! TCAATCGCAACTTGTTCTTT+CGG - tig0024623:76744-76763 None:intergenic 35.0%
TTCTTACGGATCCACAATAA+AGG - tig0024623:76964-76983 None:intergenic 35.0%
ACTTCTGATTGAATGTGTGT+TGG + tig0024623:77055-77074 Msa1418670:intron 35.0%
TTCAGAATGTTGCTCGATAT+TGG + tig0024623:77244-77263 Msa1418670:intron 35.0%
!!! AGCTTTTGAAACTCTAGACT+CGG - tig0024623:77464-77483 None:intergenic 35.0%
TGCAATTTCTGATATGCAGA+AGG + tig0024623:77504-77523 Msa1418670:CDS 35.0%
CGTTATACCATAGCAGAATT+TGG + tig0024623:77600-77619 Msa1418670:intron 35.0%
TCGAGAATAGCAGAAAATAG+TGG - tig0024623:77734-77753 None:intergenic 35.0%
CTATTCTCGATTGACAACAT+TGG + tig0024623:77744-77763 Msa1418670:intron 35.0%
! TTCAGAACTAAAAGCACAAC+TGG + tig0024623:77915-77934 Msa1418670:CDS 35.0%
!! TGATTCGAATGATTTTCAGG+TGG - tig0024623:76649-76668 None:intergenic 35.0%
ACCAAACACAACAACAACAA+TGG + tig0024623:76704-76723 Msa1418670:exon 35.0%
! ACCATTGTTGTTGTTGTGTT+TGG - tig0024623:76708-76727 None:intergenic 35.0%
ACAACAACAATGGTGAAAAC+TGG + tig0024623:76714-76733 Msa1418670:exon 35.0%
! TCAATCGCAACTTGTTCTTT+CGG - tig0024623:76744-76763 None:intergenic 35.0%
TTCTTACGGATCCACAATAA+AGG - tig0024623:76964-76983 None:intergenic 35.0%
ACTTCTGATTGAATGTGTGT+TGG + tig0024623:77055-77074 Msa1418670:intron 35.0%
TTCAGAATGTTGCTCGATAT+TGG + tig0024623:77244-77263 Msa1418670:intron 35.0%
!!! AGCTTTTGAAACTCTAGACT+CGG - tig0024623:77464-77483 None:intergenic 35.0%
TGCAATTTCTGATATGCAGA+AGG + tig0024623:77504-77523 Msa1418670:CDS 35.0%
CGTTATACCATAGCAGAATT+TGG + tig0024623:77600-77619 Msa1418670:intron 35.0%
TCGAGAATAGCAGAAAATAG+TGG - tig0024623:77734-77753 None:intergenic 35.0%
CTATTCTCGATTGACAACAT+TGG + tig0024623:77744-77763 Msa1418670:intron 35.0%
! TTCAGAACTAAAAGCACAAC+TGG + tig0024623:77915-77934 Msa1418670:CDS 35.0%
! TTGTACGTGGGACCTATTTT+CGG + tig0024623:76611-76630 Msa1418670:five_prime_UTR 40.0%
AGACTAGAGAGACCGAAAAT+AGG - tig0024623:76626-76645 None:intergenic 40.0%
GTTATTAACTCTTGTAGCGC+CGG + tig0024623:77031-77050 Msa1418670:intron 40.0%
! ACACATTCAATCAGAAGTGC+CGG - tig0024623:77053-77072 None:intergenic 40.0%
!! GCTCGATATTGGTGTTAAGT+TGG + tig0024623:77255-77274 Msa1418670:intron 40.0%
!! TTGGTGTTAAGTTGGTTGCT+TGG + tig0024623:77263-77282 Msa1418670:intron 40.0%
TTGGTTGCTTGGATAGTTGA+TGG + tig0024623:77274-77293 Msa1418670:intron 40.0%
!!! TGTTGTTGAAGGGTTTTGAG+TGG + tig0024623:77350-77369 Msa1418670:intron 40.0%
GAAAGAGAAAGCAGAGAACT+TGG + tig0024623:77372-77391 Msa1418670:CDS 40.0%
TACTTCCACAACAAGTTGCT+CGG - tig0024623:77440-77459 None:intergenic 40.0%
AGAAGGAGTTGACAGAATTG+AGG + tig0024623:77521-77540 Msa1418670:CDS 40.0%
GGTTTGTCCAAATTCTGCTA+TGG - tig0024623:77610-77629 None:intergenic 40.0%
GCTATAGCACTATTGTGTAG+CGG + tig0024623:77696-77715 Msa1418670:intron 40.0%
! TTGTACGTGGGACCTATTTT+CGG + tig0024623:76611-76630 Msa1418670:five_prime_UTR 40.0%
AGACTAGAGAGACCGAAAAT+AGG - tig0024623:76626-76645 None:intergenic 40.0%
GTTATTAACTCTTGTAGCGC+CGG + tig0024623:77031-77050 Msa1418670:intron 40.0%
! ACACATTCAATCAGAAGTGC+CGG - tig0024623:77053-77072 None:intergenic 40.0%
!! GCTCGATATTGGTGTTAAGT+TGG + tig0024623:77255-77274 Msa1418670:intron 40.0%
!! TTGGTGTTAAGTTGGTTGCT+TGG + tig0024623:77263-77282 Msa1418670:intron 40.0%
TTGGTTGCTTGGATAGTTGA+TGG + tig0024623:77274-77293 Msa1418670:intron 40.0%
!!! TGTTGTTGAAGGGTTTTGAG+TGG + tig0024623:77350-77369 Msa1418670:intron 40.0%
GAAAGAGAAAGCAGAGAACT+TGG + tig0024623:77372-77391 Msa1418670:CDS 40.0%
TACTTCCACAACAAGTTGCT+CGG - tig0024623:77440-77459 None:intergenic 40.0%
AGAAGGAGTTGACAGAATTG+AGG + tig0024623:77521-77540 Msa1418670:CDS 40.0%
GGTTTGTCCAAATTCTGCTA+TGG - tig0024623:77610-77629 None:intergenic 40.0%
GCTATAGCACTATTGTGTAG+CGG + tig0024623:77696-77715 Msa1418670:intron 40.0%
AAACGCCATCTTCTCGAAGA+AGG + tig0024623:76795-76814 Msa1418670:CDS 45.0%
! CTAGAAAGTGCGAGAAAAGC+AGG - tig0024623:76831-76850 None:intergenic 45.0%
TTCTAGACCCATCGTTTCTC+AGG + tig0024623:76845-76864 Msa1418670:CDS 45.0%
GTACGTACCTGAGAAACGAT+GGG - tig0024623:76855-76874 None:intergenic 45.0%
! TGTTGGTGTCTGACATGTGT+CGG + tig0024623:77072-77091 Msa1418670:intron 45.0%
GAAAGCAGAGAACTTGGAAG+TGG + tig0024623:77378-77397 Msa1418670:CDS 45.0%
!! GTTATCCGAGCAACTTGTTG+TGG + tig0024623:77432-77451 Msa1418670:CDS 45.0%
TTGTCCAATAGCGGCTATAG+TGG + tig0024623:77666-77685 Msa1418670:intron 45.0%
AAACGCCATCTTCTCGAAGA+AGG + tig0024623:76795-76814 Msa1418670:CDS 45.0%
! CTAGAAAGTGCGAGAAAAGC+AGG - tig0024623:76831-76850 None:intergenic 45.0%
TTCTAGACCCATCGTTTCTC+AGG + tig0024623:76845-76864 Msa1418670:CDS 45.0%
GTACGTACCTGAGAAACGAT+GGG - tig0024623:76855-76874 None:intergenic 45.0%
! TGTTGGTGTCTGACATGTGT+CGG + tig0024623:77072-77091 Msa1418670:intron 45.0%
GAAAGCAGAGAACTTGGAAG+TGG + tig0024623:77378-77397 Msa1418670:CDS 45.0%
!! GTTATCCGAGCAACTTGTTG+TGG + tig0024623:77432-77451 Msa1418670:CDS 45.0%
TTGTCCAATAGCGGCTATAG+TGG + tig0024623:77666-77685 Msa1418670:intron 45.0%
GGTACGTACCTGAGAAACGA+TGG - tig0024623:76856-76875 None:intergenic 50.0%
AGCACCACTATAGCCGCTAT+TGG - tig0024623:77673-77692 None:intergenic 50.0%
GGTACGTACCTGAGAAACGA+TGG - tig0024623:76856-76875 None:intergenic 50.0%
AGCACCACTATAGCCGCTAT+TGG - tig0024623:77673-77692 None:intergenic 50.0%
GAGCGCCTTCTTCGAGAAGA+TGG - tig0024623:76803-76822 None:intergenic 55.0%
GAGCGCCTTCTTCGAGAAGA+TGG - tig0024623:76803-76822 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0024623 gene 76591 78038 76591 ID=Msa1418670;Name=Msa1418670
tig0024623 mRNA 76591 78038 76591 ID=Msa1418670-mRNA-1;Parent=Msa1418670;Name=Msa1418670-mRNA-1;_AED=0.13;_eAED=0.13;_QI=132|1|0.66|1|1|1|3|0|174
tig0024623 exon 76591 76866 76591 ID=Msa1418670-mRNA-1:exon:449;Parent=Msa1418670-mRNA-1
tig0024623 exon 77361 77542 77361 ID=Msa1418670-mRNA-1:exon:450;Parent=Msa1418670-mRNA-1
tig0024623 exon 77843 78038 77843 ID=Msa1418670-mRNA-1:exon:451;Parent=Msa1418670-mRNA-1
tig0024623 five_prime_UTR 76591 76722 76591 ID=Msa1418670-mRNA-1:five_prime_utr;Parent=Msa1418670-mRNA-1
tig0024623 CDS 76723 76866 76723 ID=Msa1418670-mRNA-1:cds;Parent=Msa1418670-mRNA-1
tig0024623 CDS 77361 77542 77361 ID=Msa1418670-mRNA-1:cds;Parent=Msa1418670-mRNA-1
tig0024623 CDS 77843 78038 77843 ID=Msa1418670-mRNA-1:cds;Parent=Msa1418670-mRNA-1
Gene Sequence

>Msa1418670

ATGGTGAAAACTGGAAGACCGAAAGAACAAGTTGCGATTGATGCAATCAATTATGCTATGAAAGCTCTCAAGAAACGCCATCTTCTCGAAGAAGGCGCTCATGCTCCTGCTTTTCTCGCACTTTCTAGACCCATCGTTTCTCAGGGTTTTGAGTGGAAAGAGAAAGCAGAGAACTTGGAAGTGGAACTTCAGCAATGCTATAAAGCTCAATCTCGGTTATCCGAGCAACTTGTTGTGGAAGTAGCCGAGTCTAGAGTTTCAAAAGCTTCCCTTCAAGAGAAAGAAAATGCAATTTCTGATATGCAGAAGGAGTTGACAGAATTGAGGGATGAGTGCTCTCAATTAAAGACAGATTTGGAGCAAAAGACTAAAGAAGTAGAATTAGTTCTTAGCGAGAATTCAGAACTAAAAGCACAACTGGAGCAGATGACTACTAAAGCTAATGAAGCTGAAGCTGAAAATAAGATGTTGATTGACCGCTTGATGTTAGAAAAGATGAAGGATGCTGAACGCCTAAATGAG

Protein sequence

>Msa1418670

MVKTGRPKEQVAIDAINYAMKALKKRHLLEEGAHAPAFLALSRPIVSQGFEWKEKAENLEVELQQCYKAQSRLSEQLVVEVAESRVSKASLQEKENAISDMQKELTELRDECSQLKTDLEQKTKEVELVLSENSELKAQLEQMTTKANEAEAENKMLIDRLMLEKMKDAERLNE