Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419740 | sp|Q9SJM6|SAP4_ARATH | 59.412 | 170 | 60 | 3 | 1 | 170 | 1 | 161 | 2.05e-64 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419740 | G7L614 | 97.647 | 170 | 2 | 1 | 1 | 170 | 1 | 168 | 3.40e-117 | 338 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0725480 | Msa1419740 | 0.805123 | 1.553920e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419740 | MtrunA17_Chr7g0266121 | 97.647 | 170 | 2 | 1 | 1 | 170 | 1 | 168 | 6.54e-121 | 338 |
| Msa1419740 | MtrunA17_Chr1g0178851 | 58.824 | 170 | 56 | 3 | 1 | 170 | 1 | 156 | 4.49e-60 | 183 |
| Msa1419740 | MtrunA17_Chr4g0026561 | 53.672 | 177 | 68 | 5 | 1 | 170 | 1 | 170 | 2.88e-59 | 182 |
| Msa1419740 | MtrunA17_Chr2g0321261 | 51.765 | 170 | 62 | 5 | 1 | 170 | 5 | 154 | 8.00e-55 | 170 |
| Msa1419740 | MtrunA17_Chr1g0173971 | 44.706 | 170 | 87 | 3 | 3 | 170 | 5 | 169 | 2.80e-47 | 151 |
| Msa1419740 | MtrunA17_Chr2g0328941 | 44.910 | 167 | 80 | 4 | 10 | 170 | 12 | 172 | 3.63e-46 | 149 |
| Msa1419740 | MtrunA17_Chr7g0258071 | 43.353 | 173 | 88 | 2 | 3 | 170 | 93 | 260 | 1.43e-45 | 150 |
| Msa1419740 | MtrunA17_Chr4g0032621 | 39.674 | 184 | 95 | 2 | 3 | 170 | 4 | 187 | 5.73e-44 | 144 |
| Msa1419740 | MtrunA17_Chr7g0274001 | 39.610 | 154 | 66 | 4 | 15 | 168 | 5 | 131 | 1.26e-30 | 108 |
| Msa1419740 | MtrunA17_Chr1g0203591 | 37.908 | 153 | 75 | 5 | 15 | 167 | 5 | 137 | 2.49e-29 | 105 |
| Msa1419740 | MtrunA17_Chr3g0087831 | 33.742 | 163 | 74 | 3 | 15 | 170 | 28 | 163 | 1.75e-26 | 98.6 |
| Msa1419740 | MtrunA17_Chr8g0351971 | 59.677 | 62 | 25 | 0 | 109 | 170 | 179 | 240 | 2.14e-24 | 95.1 |
| Msa1419740 | MtrunA17_Chr2g0306911 | 37.255 | 153 | 62 | 4 | 15 | 167 | 6 | 124 | 2.78e-24 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1419740 | AT2G36320.1 | 59.412 | 170 | 60 | 3 | 1 | 170 | 1 | 161 | 2.08e-65 | 197 |
| Msa1419740 | AT3G52800.2 | 53.757 | 173 | 74 | 3 | 1 | 170 | 1 | 170 | 7.23e-52 | 163 |
| Msa1419740 | AT3G52800.1 | 53.757 | 173 | 74 | 3 | 1 | 170 | 1 | 170 | 7.23e-52 | 163 |
| Msa1419740 | AT2G27580.2 | 50.292 | 171 | 76 | 4 | 1 | 170 | 1 | 163 | 1.39e-49 | 157 |
| Msa1419740 | AT2G27580.1 | 50.292 | 171 | 76 | 4 | 1 | 170 | 1 | 163 | 1.39e-49 | 157 |
| Msa1419740 | AT4G22820.1 | 49.080 | 163 | 71 | 4 | 14 | 170 | 20 | 176 | 2.39e-49 | 157 |
| Msa1419740 | AT4G22820.2 | 49.080 | 163 | 71 | 4 | 14 | 170 | 20 | 176 | 2.39e-49 | 157 |
| Msa1419740 | AT4G12040.2 | 47.619 | 168 | 76 | 4 | 10 | 170 | 13 | 175 | 4.63e-47 | 151 |
| Msa1419740 | AT4G12040.1 | 47.619 | 168 | 76 | 4 | 10 | 170 | 13 | 175 | 4.63e-47 | 151 |
| Msa1419740 | AT1G12440.2 | 44.706 | 170 | 73 | 3 | 9 | 170 | 12 | 168 | 8.33e-43 | 140 |
| Msa1419740 | AT1G12440.1 | 44.706 | 170 | 73 | 3 | 9 | 170 | 12 | 168 | 8.33e-43 | 140 |
| Msa1419740 | AT1G12440.3 | 44.706 | 170 | 73 | 3 | 9 | 170 | 12 | 168 | 8.33e-43 | 140 |
| Msa1419740 | AT1G51200.4 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 3.07e-40 | 134 |
| Msa1419740 | AT1G51200.1 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 3.07e-40 | 134 |
| Msa1419740 | AT1G51200.3 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 3.07e-40 | 134 |
| Msa1419740 | AT1G51200.2 | 46.707 | 167 | 78 | 3 | 10 | 170 | 12 | 173 | 3.07e-40 | 134 |
| Msa1419740 | AT4G25380.1 | 36.129 | 155 | 65 | 4 | 16 | 170 | 10 | 130 | 2.19e-27 | 100 |
| Msa1419740 | AT3G12630.1 | 34.211 | 152 | 76 | 4 | 21 | 170 | 31 | 160 | 1.80e-24 | 93.6 |
| Msa1419740 | AT4G14225.1 | 35.032 | 157 | 63 | 4 | 15 | 170 | 7 | 125 | 5.02e-23 | 89.0 |
Find 50 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGCAGAGTCTGTGGCCTTC+TGG | 0.265748 | tig0024727:-3854 | None:intergenic |
| CTTGTTCTTGTTCCTTCAAA+TGG | 0.292468 | tig0024727:-3943 | None:intergenic |
| TGTGAATTGTCCGATGCTTC+AGG | 0.360056 | tig0024727:-4112 | None:intergenic |
| AACGCCGGTGGTTGAGGTTG+AGG | 0.369798 | tig0024727:-4070 | None:intergenic |
| AGAAAGAGGACCGGGTTAAC+CGG | 0.386243 | tig0024727:+4169 | Msa1419740:CDS |
| GGAGATAGCGAAAGCGAATC+CGG | 0.416264 | tig0024727:+4285 | Msa1419740:CDS |
| GCAAACAACTGCGGCTTCTT+CGG | 0.427864 | tig0024727:+3875 | Msa1419740:CDS |
| TTGTGGGTCCCACAGGTATC+CGG | 0.444625 | tig0024727:+4219 | Msa1419740:CDS |
| AGAGATTCGATTTCCGCAAC+GGG | 0.444842 | tig0024727:-4046 | None:intergenic |
| AGCTTATCAGCTTTGATCAC+CGG | 0.447035 | tig0024727:-4304 | None:intergenic |
| AACGAACACGGCGGTGGAAG+CGG | 0.470759 | tig0024727:-4014 | None:intergenic |
| CGGTTCAATCGAACCGGTGC+GGG | 0.475968 | tig0024727:+4140 | Msa1419740:CDS |
| AACTAAATCAACAATCGAAA+CGG | 0.478568 | tig0024727:+3970 | Msa1419740:CDS |
| ACGGTCAACGCCGGTGGTTG+AGG | 0.481127 | tig0024727:-4076 | None:intergenic |
| AGCAGCAACGAACACGGCGG+TGG | 0.486244 | tig0024727:-4020 | None:intergenic |
| CCGCACCGGTTCGATTGAAC+CGG | 0.490138 | tig0024727:-4139 | None:intergenic |
| TTACAGAGACATCCATTTGA+AGG | 0.490147 | tig0024727:+3931 | Msa1419740:CDS |
| ACGCCGGTGGTTGAGGTTGA+GGG | 0.510233 | tig0024727:-4069 | None:intergenic |
| CGTTGCTGCTTCGCCCGTTG+CGG | 0.514245 | tig0024727:+4033 | Msa1419740:CDS |
| ATCGGACAATTCACAAGGAC+CGG | 0.522802 | tig0024727:+4120 | Msa1419740:CDS |
| GGTGGAAGCGGATGCTGAGG+CGG | 0.523382 | tig0024727:-4002 | None:intergenic |
| AAACCGCACTCATGTTTCTC+CGG | 0.524064 | tig0024727:-4238 | None:intergenic |
| TTTCAAAGCAGTGGGGAGAG+AGG | 0.529533 | tig0024727:+4264 | Msa1419740:CDS |
| TCTTTCTACACGTCCCGCAC+CGG | 0.534045 | tig0024727:-4153 | None:intergenic |
| GAAAGAGGACCGGGTTAACC+GGG | 0.537492 | tig0024727:+4170 | Msa1419740:CDS |
| AAGGACCGGTTCAATCGAAC+CGG | 0.537575 | tig0024727:+4134 | Msa1419740:CDS |
| GGAAGCGGATGCTGAGGCGG+AGG | 0.542625 | tig0024727:-3999 | None:intergenic |
| AGTTGTTTGCGCAGAGTCTG+TGG | 0.555837 | tig0024727:-3862 | None:intergenic |
| GGGCGAAGCAGCAACGAACA+CGG | 0.557556 | tig0024727:-4026 | None:intergenic |
| ACACTTGAACCCGGTTAACC+CGG | 0.561755 | tig0024727:-4179 | None:intergenic |
| GGCGGTGGAAGCGGATGCTG+AGG | 0.566120 | tig0024727:-4005 | None:intergenic |
| GAGAGATTCGATTTCCGCAA+CGG | 0.574585 | tig0024727:-4047 | None:intergenic |
| CATGTTTCTCCGGATACCTG+TGG | 0.575785 | tig0024727:-4228 | None:intergenic |
| GGTGCGGGACGTGTAGAAAG+AGG | 0.587813 | tig0024727:+4155 | Msa1419740:CDS |
| ACCGGGTTCAAGTGTAGATG+TGG | 0.588078 | tig0024727:+4187 | Msa1419740:CDS |
| GACGGTACGGTCAACGCCGG+TGG | 0.590519 | tig0024727:-4082 | None:intergenic |
| TCCACATCTACACTTGAACC+CGG | 0.592518 | tig0024727:-4188 | None:intergenic |
| GGGACGTGTAGAAAGAGGAC+CGG | 0.594127 | tig0024727:+4160 | Msa1419740:CDS |
| GGACGTGTAGAAAGAGGACC+GGG | 0.606988 | tig0024727:+4161 | Msa1419740:CDS |
| ATGTTTCTCCGGATACCTGT+GGG | 0.618435 | tig0024727:-4227 | None:intergenic |
| TTCAGGCACGATCGACGGTA+CGG | 0.620849 | tig0024727:-4095 | None:intergenic |
| TCTCCCTCAACCTCAACCAC+CGG | 0.644565 | tig0024727:+4066 | Msa1419740:CDS |
| GATGCTTCAGGCACGATCGA+CGG | 0.657260 | tig0024727:-4100 | None:intergenic |
| ATCGACGGTACGGTCAACGC+CGG | 0.667919 | tig0024727:-4085 | None:intergenic |
| CCGGTTCAATCGAACCGGTG+CGG | 0.668012 | tig0024727:+4139 | Msa1419740:CDS |
| CATCGATGTGAAACTCCAGA+AGG | 0.699805 | tig0024727:+3839 | Msa1419740:CDS |
| GAAGCATCGGACAATTCACA+AGG | 0.714221 | tig0024727:+4115 | Msa1419740:CDS |
| AGACTCTGCGCAAACAACTG+CGG | 0.735745 | tig0024727:+3866 | Msa1419740:CDS |
| TATCCGGAGAAACATGAGTG+CGG | 0.743977 | tig0024727:+4235 | Msa1419740:CDS |
| CGAAGCAGCAACGAACACGG+CGG | 0.748812 | tig0024727:-4023 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTAAATCAACAATCGAAA+CGG | + | tig0024727:3970-3989 | Msa1419740:CDS | 25.0% |
| TTACAGAGACATCCATTTGA+AGG | + | tig0024727:3931-3950 | Msa1419740:CDS | 35.0% | |
| CTTGTTCTTGTTCCTTCAAA+TGG | - | tig0024727:3946-3965 | None:intergenic | 35.0% | |
| ! | AGATGTGGAATAACGTTTTG+TGG | + | tig0024727:4202-4221 | Msa1419740:CDS | 35.0% |
| ! | GATGTGGAATAACGTTTTGT+GGG | + | tig0024727:4203-4222 | Msa1419740:CDS | 35.0% |
| !!! | GGTTTTGATTTCAAAGCAGT+GGG | + | tig0024727:4256-4275 | Msa1419740:CDS | 35.0% |
| !!! | GTTTTGATTTCAAAGCAGTG+GGG | + | tig0024727:4257-4276 | Msa1419740:CDS | 35.0% |
| !!! | CGGTTTTGATTTCAAAGCAG+TGG | + | tig0024727:4255-4274 | Msa1419740:CDS | 40.0% |
| ! | AGCTTATCAGCTTTGATCAC+CGG | - | tig0024727:4307-4326 | None:intergenic | 40.0% |
| CATCGATGTGAAACTCCAGA+AGG | + | tig0024727:3839-3858 | Msa1419740:CDS | 45.0% | |
| AGAGATTCGATTTCCGCAAC+GGG | - | tig0024727:4049-4068 | None:intergenic | 45.0% | |
| GAGAGATTCGATTTCCGCAA+CGG | - | tig0024727:4050-4069 | None:intergenic | 45.0% | |
| TGTGAATTGTCCGATGCTTC+AGG | - | tig0024727:4115-4134 | None:intergenic | 45.0% | |
| !! | GAAGCATCGGACAATTCACA+AGG | + | tig0024727:4115-4134 | Msa1419740:CDS | 45.0% |
| ATCGGACAATTCACAAGGAC+CGG | + | tig0024727:4120-4139 | Msa1419740:CDS | 45.0% | |
| TCCACATCTACACTTGAACC+CGG | - | tig0024727:4191-4210 | None:intergenic | 45.0% | |
| ATGTTTCTCCGGATACCTGT+GGG | - | tig0024727:4230-4249 | None:intergenic | 45.0% | |
| TATCCGGAGAAACATGAGTG+CGG | + | tig0024727:4235-4254 | Msa1419740:CDS | 45.0% | |
| AAACCGCACTCATGTTTCTC+CGG | - | tig0024727:4241-4260 | None:intergenic | 45.0% | |
| AGTTGTTTGCGCAGAGTCTG+TGG | - | tig0024727:3865-3884 | None:intergenic | 50.0% | |
| AGACTCTGCGCAAACAACTG+CGG | + | tig0024727:3866-3885 | Msa1419740:CDS | 50.0% | |
| GCAAACAACTGCGGCTTCTT+CGG | + | tig0024727:3875-3894 | Msa1419740:CDS | 50.0% | |
| ! | AAGGACCGGTTCAATCGAAC+CGG | + | tig0024727:4134-4153 | Msa1419740:CDS | 50.0% |
| AGAAAGAGGACCGGGTTAAC+CGG | + | tig0024727:4169-4188 | Msa1419740:CDS | 50.0% | |
| ACACTTGAACCCGGTTAACC+CGG | - | tig0024727:4182-4201 | None:intergenic | 50.0% | |
| ACCGGGTTCAAGTGTAGATG+TGG | + | tig0024727:4187-4206 | Msa1419740:CDS | 50.0% | |
| ! | TAACGTTTTGTGGGTCCCAC+AGG | + | tig0024727:4212-4231 | Msa1419740:CDS | 50.0% |
| CATGTTTCTCCGGATACCTG+TGG | - | tig0024727:4231-4250 | None:intergenic | 50.0% | |
| TTTCAAAGCAGTGGGGAGAG+AGG | + | tig0024727:4264-4283 | Msa1419740:CDS | 50.0% | |
| GGAGATAGCGAAAGCGAATC+CGG | + | tig0024727:4285-4304 | Msa1419740:CDS | 50.0% | |
| TCTCCCTCAACCTCAACCAC+CGG | + | tig0024727:4066-4085 | Msa1419740:CDS | 55.0% | |
| TTCAGGCACGATCGACGGTA+CGG | - | tig0024727:4098-4117 | None:intergenic | 55.0% | |
| GATGCTTCAGGCACGATCGA+CGG | - | tig0024727:4103-4122 | None:intergenic | 55.0% | |
| GTCGATCGTGCCTGAAGCAT+CGG | + | tig0024727:4102-4121 | Msa1419740:CDS | 55.0% | |
| TCTTTCTACACGTCCCGCAC+CGG | - | tig0024727:4156-4175 | None:intergenic | 55.0% | |
| GGGACGTGTAGAAAGAGGAC+CGG | + | tig0024727:4160-4179 | Msa1419740:CDS | 55.0% | |
| GGACGTGTAGAAAGAGGACC+GGG | + | tig0024727:4161-4180 | Msa1419740:CDS | 55.0% | |
| GAAAGAGGACCGGGTTAACC+GGG | + | tig0024727:4170-4189 | Msa1419740:CDS | 55.0% | |
| TTGTGGGTCCCACAGGTATC+CGG | + | tig0024727:4219-4238 | Msa1419740:CDS | 55.0% | |
| AACGAACACGGCGGTGGAAG+CGG | - | tig0024727:4017-4036 | None:intergenic | 60.0% | |
| CGAAGCAGCAACGAACACGG+CGG | - | tig0024727:4026-4045 | None:intergenic | 60.0% | |
| GGGCGAAGCAGCAACGAACA+CGG | - | tig0024727:4029-4048 | None:intergenic | 60.0% | |
| ACGCCGGTGGTTGAGGTTGA+GGG | - | tig0024727:4072-4091 | None:intergenic | 60.0% | |
| AACGCCGGTGGTTGAGGTTG+AGG | - | tig0024727:4073-4092 | None:intergenic | 60.0% | |
| ATCGACGGTACGGTCAACGC+CGG | - | tig0024727:4088-4107 | None:intergenic | 60.0% | |
| !! | CCGCACCGGTTCGATTGAAC+CGG | - | tig0024727:4142-4161 | None:intergenic | 60.0% |
| CCGGTTCAATCGAACCGGTG+CGG | + | tig0024727:4139-4158 | Msa1419740:CDS | 60.0% | |
| CGGTTCAATCGAACCGGTGC+GGG | + | tig0024727:4140-4159 | Msa1419740:CDS | 60.0% | |
| GGTGCGGGACGTGTAGAAAG+AGG | + | tig0024727:4155-4174 | Msa1419740:CDS | 60.0% | |
| GCGCAGAGTCTGTGGCCTTC+TGG | - | tig0024727:3857-3876 | None:intergenic | 65.0% | |
| GGTGGAAGCGGATGCTGAGG+CGG | - | tig0024727:4005-4024 | None:intergenic | 65.0% | |
| AGCAGCAACGAACACGGCGG+TGG | - | tig0024727:4023-4042 | None:intergenic | 65.0% | |
| ! | CGTTGCTGCTTCGCCCGTTG+CGG | + | tig0024727:4033-4052 | Msa1419740:CDS | 65.0% |
| ACGGTCAACGCCGGTGGTTG+AGG | - | tig0024727:4079-4098 | None:intergenic | 65.0% | |
| GGAAGCGGATGCTGAGGCGG+AGG | - | tig0024727:4002-4021 | None:intergenic | 70.0% | |
| GGCGGTGGAAGCGGATGCTG+AGG | - | tig0024727:4008-4027 | None:intergenic | 70.0% | |
| GACGGTACGGTCAACGCCGG+TGG | - | tig0024727:4085-4104 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024727 | gene | 3827 | 4339 | 3827 | ID=Msa1419740;Name=Msa1419740 |
| tig0024727 | mRNA | 3827 | 4339 | 3827 | ID=Msa1419740-mRNA-1;Parent=Msa1419740;Name=Msa1419740-mRNA-1;_AED=0.28;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|170 |
| tig0024727 | exon | 3827 | 4339 | 3827 | ID=Msa1419740-mRNA-1:exon:1061;Parent=Msa1419740-mRNA-1 |
| tig0024727 | CDS | 3827 | 4339 | 3827 | ID=Msa1419740-mRNA-1:cds;Parent=Msa1419740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
