AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1425950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1425950 MtrunA17_Chr8g0347121 90.076 131 13 0 1 131 1 131 2.55e-76 239
Msa1425950 MtrunA17_Chr7g0275581 49.032 155 54 3 1 131 1 154 4.27e-41 144
Msa1425950 MtrunA17_Chr3g0126541 43.421 152 65 2 1 131 1 152 5.21e-32 119
Msa1425950 MtrunA17_Chr1g0158861 41.333 150 66 3 4 131 5 154 3.26e-31 116
Msa1425950 MtrunA17_Chr2g0326871 39.000 100 58 2 34 131 49 147 2.01e-19 82.8
Msa1425950 MtrunA17_Chr4g0073941 41.667 96 54 1 36 131 77 170 2.56e-18 79.7
Msa1425950 MtrunA17_Chr1g0181121 35.238 105 60 3 34 131 11 114 4.69e-17 76.3
Msa1425950 MtrunA17_Chr4g0028861 27.481 131 93 2 1 131 66 194 3.07e-16 73.9
Msa1425950 MtrunA17_Chr7g0228181 34.653 101 63 2 33 131 15 114 1.61e-15 71.6
Msa1425950 MtrunA17_Chr2g0305771 34.177 79 48 1 57 131 40 118 1.96e-15 71.6
Msa1425950 MtrunA17_Chr4g0001481 40.625 96 55 1 36 131 79 172 2.56e-15 71.2
Msa1425950 MtrunA17_Chr6g0480851 34.247 73 48 0 59 131 183 255 9.80e-14 66.6
Msa1425950 MtrunA17_Chr6g0480781 34.247 73 48 0 59 131 168 240 6.61e-13 64.3
Msa1425950 MtrunA17_Chr6g0480741 36.111 72 46 0 60 131 115 186 2.14e-12 62.8
Msa1425950 MtrunA17_Chr6g0480671 36.111 72 46 0 60 131 193 264 3.52e-12 62.4
Msa1425950 MtrunA17_Chr3g0125651 36.111 72 45 1 60 131 36 106 3.99e-12 62.0
Msa1425950 MtrunA17_Chr2g0318551 34.722 72 47 0 60 131 63 134 8.08e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1425950 AT5G53130.1 42.581 155 65 4 1 131 1 155 9.39e-32 118
Msa1425950 AT4G01010.1 40.714 140 73 3 1 131 1 139 4.87e-30 113
Msa1425950 AT4G01010.2 40.714 140 73 3 1 131 1 139 4.87e-30 113
Msa1425950 AT1G01340.2 55.128 78 35 0 54 131 62 139 1.28e-25 100
Msa1425950 AT1G01340.1 55.128 78 35 0 54 131 57 134 1.34e-25 100
Msa1425950 AT1G19780.1 41.304 92 52 1 40 131 79 168 5.36e-21 87.8
Msa1425950 AT1G15990.1 32.051 156 81 4 1 131 7 162 1.14e-19 84.0
Msa1425950 AT2G46430.1 48.611 72 37 0 60 131 72 143 1.06e-18 81.3
Msa1425950 AT2G46430.2 48.611 72 37 0 60 131 72 143 1.06e-18 81.3
Msa1425950 AT5G57940.1 44.578 83 44 1 49 131 79 159 1.17e-17 78.2
Msa1425950 AT5G57940.2 44.578 83 44 1 49 131 79 159 1.17e-17 78.2
Msa1425950 AT5G57940.3 44.578 83 44 1 49 131 72 152 1.19e-17 78.2
Msa1425950 AT4G30560.1 37.500 96 58 1 36 131 81 174 4.21e-15 70.9
Msa1425950 AT4G30560.4 37.500 96 58 1 36 131 81 174 4.21e-15 70.9
Msa1425950 AT4G30560.5 37.500 96 58 1 36 131 55 148 4.35e-15 70.9
Msa1425950 AT4G30560.2 37.500 96 58 1 36 131 55 148 4.35e-15 70.9
Msa1425950 AT4G30560.3 37.500 96 58 1 36 131 55 148 4.35e-15 70.9
Msa1425950 AT3G48010.1 35.897 78 50 0 54 131 38 115 1.28e-14 69.7
Msa1425950 AT2G28260.1 33.333 93 59 2 39 131 49 138 1.53e-14 69.3
Msa1425950 AT2G28260.2 33.333 93 59 2 39 131 9 98 1.70e-14 69.3
Msa1425950 AT2G23980.6 38.158 76 47 0 56 131 72 147 6.26e-14 67.8
Msa1425950 AT2G23980.9 38.158 76 47 0 56 131 99 174 6.34e-14 67.8
Msa1425950 AT2G23980.10 38.158 76 47 0 56 131 99 174 6.34e-14 67.8
Msa1425950 AT2G23980.1 38.158 76 47 0 56 131 99 174 6.34e-14 67.8
Msa1425950 AT2G23980.4 38.158 76 47 0 56 131 99 174 6.34e-14 67.8
Msa1425950 AT2G23980.5 38.158 76 47 0 56 131 99 174 6.34e-14 67.8
Msa1425950 AT2G23980.3 38.158 76 47 0 56 131 99 174 6.49e-14 67.4
Msa1425950 AT2G23980.2 38.158 76 47 0 56 131 99 174 6.49e-14 67.4
Msa1425950 AT2G23980.8 38.158 76 47 0 56 131 99 174 6.49e-14 67.4
Msa1425950 AT2G23980.7 38.158 76 47 0 56 131 99 174 6.49e-14 67.4
Msa1425950 AT2G46440.1 43.548 62 35 0 68 129 37 98 7.31e-14 67.4
Msa1425950 AT2G46450.4 43.548 62 35 0 68 129 37 98 7.80e-14 67.4
Msa1425950 AT2G46450.1 43.548 62 35 0 68 129 37 98 7.80e-14 67.4
Msa1425950 AT4G30360.1 34.667 75 47 1 59 131 70 144 1.12e-12 63.9
Msa1425950 AT2G24610.1 35.065 77 48 1 57 131 69 145 2.55e-12 63.2
Msa1425950 AT5G14870.1 33.333 75 49 1 57 131 37 110 2.59e-12 63.2
Msa1425950 AT2G24610.2 35.065 77 48 1 57 131 84 160 2.84e-12 62.8

Find 31 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGTTCCATTCAAATTA+AGG 0.206765 tig0025908:+34797 None:intergenic
TGCAATATAAATAATGAAAT+TGG 0.233761 tig0025908:-35863 Msa1425950:five_prime_UTR
ATAAATTAAATTACGAATAA+TGG 0.247420 tig0025908:+33877 None:intergenic
AGATTACTGCTAGTGTTCTT+TGG 0.306117 tig0025908:-34774 Msa1425950:CDS
CAATATGATTCTTCAATTGA+TGG 0.309646 tig0025908:-35027 Msa1425950:CDS
TTCCATATATAACAAAATAT+TGG 0.312357 tig0025908:-34198 Msa1425950:three_prime_UTR
TTCTGTCCAGATTTGATGAT+TGG 0.338850 tig0025908:-35080 Msa1425950:intron
GGAACTGAGCCTTGTGGAAT+TGG 0.348268 tig0025908:+34217 None:intergenic
GAATTAATTTATGTATGAAT+TGG 0.352524 tig0025908:-33989 Msa1425950:three_prime_UTR
ACAGGGATGTAAAAGAATAA+TGG 0.368359 tig0025908:+34841 None:intergenic
ATGTGTGATGGCAGTATCTA+TGG 0.374144 tig0025908:-34866 Msa1425950:CDS
ATCAATTCTTCCACTTCCTC+AGG 0.380821 tig0025908:-34593 Msa1425950:intron
AGAAAATGCCTTAATTTGAA+TGG 0.388519 tig0025908:-34805 Msa1425950:CDS
AAGGTCCACTACTTCAGAAA+TGG 0.424933 tig0025908:-34909 Msa1425950:CDS
TATCTCATTGCTATAGCAAA+AGG 0.451371 tig0025908:+34646 None:intergenic
GTATAGATTCACATAAATCT+TGG 0.467041 tig0025908:+34141 None:intergenic
TATTCTGAAACCTGAGGAAG+TGG 0.479731 tig0025908:+34583 None:intergenic
TTTCTCTGGCCATCAATCAC+AGG 0.480949 tig0025908:+34823 None:intergenic
CTCAAATTCTAATTTGAAGA+CGG 0.483730 tig0025908:-35796 Msa1425950:five_prime_UTR
TTGTTCCATTTCTGAAGTAG+TGG 0.494112 tig0025908:+34904 None:intergenic
TTAACTGAGACAGCATGGGC+TGG 0.523523 tig0025908:-34084 Msa1425950:three_prime_UTR
TTCTGAAGTAGTGGACCTTG+TGG 0.543372 tig0025908:+34913 None:intergenic
TTCTCTGGCCATCAATCACA+GGG 0.581148 tig0025908:+34824 None:intergenic
GTATATTAACTGAGACAGCA+TGG 0.590115 tig0025908:-34089 Msa1425950:three_prime_UTR
CATAATTAGAATTTGCTGCA+TGG 0.592070 tig0025908:+35825 None:intergenic
TATATTAACTGAGACAGCAT+GGG 0.593271 tig0025908:-34088 Msa1425950:three_prime_UTR
CTTTGTTATAACATGTGTGA+TGG 0.634441 tig0025908:-34878 Msa1425950:CDS
CAAATGAAAAGAAATGTGTG+AGG 0.650253 tig0025908:-35706 Msa1425950:intron
GCTATGATTCCAATTCCACA+AGG 0.677659 tig0025908:-34226 Msa1425950:three_prime_UTR
AGTAGTGTTCTTGATCCACA+AGG 0.717963 tig0025908:-34928 Msa1425950:CDS
ATATATGGAACTGAGCCTTG+TGG 0.739194 tig0025908:+34211 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAAATTACGAATAA+TGG + tig0025908:35803-35822 None:intergenic 10.0%
!! ATAAATTAAATTACGAATAA+TGG + tig0025908:35803-35822 None:intergenic 10.0%
!! TGCAATATAAATAATGAAAT+TGG - tig0025908:33814-33833 Msa1425950:three_prime_UTR 15.0%
!! TAAAATTGCATATATGAATT+CGG - tig0025908:34168-34187 Msa1425950:three_prime_UTR 15.0%
!! TTCCATATATAACAAAATAT+TGG - tig0025908:35479-35498 Msa1425950:intron 15.0%
!!! AACCAATATTTTGTTATATA+TGG + tig0025908:35484-35503 None:intergenic 15.0%
!! GAATTAATTTATGTATGAAT+TGG - tig0025908:35688-35707 Msa1425950:intron 15.0%
!! TGCAATATAAATAATGAAAT+TGG - tig0025908:33814-33833 Msa1425950:three_prime_UTR 15.0%
!! TAAAATTGCATATATGAATT+CGG - tig0025908:34168-34187 Msa1425950:three_prime_UTR 15.0%
!! TTCCATATATAACAAAATAT+TGG - tig0025908:35479-35498 Msa1425950:intron 15.0%
!!! AACCAATATTTTGTTATATA+TGG + tig0025908:35484-35503 None:intergenic 15.0%
!! GAATTAATTTATGTATGAAT+TGG - tig0025908:35688-35707 Msa1425950:intron 15.0%
!! AAATTGAAAAATAACCAACT+AGG + tig0025908:34511-34530 None:intergenic 20.0%
!!! AATGATTCATTATTTTTTGC+TGG + tig0025908:34710-34729 None:intergenic 20.0%
!! TAAGTTACCAATGTATTAAA+AGG - tig0025908:35211-35230 Msa1425950:intron 20.0%
!!! TTAAATTTTGTGCATTCAAT+CGG + tig0025908:35847-35866 None:intergenic 20.0%
!! AAATTGAAAAATAACCAACT+AGG + tig0025908:34511-34530 None:intergenic 20.0%
!!! AATGATTCATTATTTTTTGC+TGG + tig0025908:34710-34729 None:intergenic 20.0%
!! TAAGTTACCAATGTATTAAA+AGG - tig0025908:35211-35230 Msa1425950:intron 20.0%
!!! TTAAATTTTGTGCATTCAAT+CGG + tig0025908:35847-35866 None:intergenic 20.0%
! CTCAAATTCTAATTTGAAGA+CGG - tig0025908:33881-33900 Msa1425950:three_prime_UTR 25.0%
! CTGATTCAAAAGAATATGTA+TGG + tig0025908:34071-34090 None:intergenic 25.0%
!! TTCTTTTGTTCAGGAAAAAA+AGG - tig0025908:34106-34125 Msa1425950:three_prime_UTR 25.0%
! TTTAGTCACTCATAGTAATA+TGG + tig0025908:34438-34457 None:intergenic 25.0%
! GTAAACTAAGCATAAATCTA+TGG - tig0025908:34481-34500 Msa1425950:intron 25.0%
! CAATATGATTCTTCAATTGA+TGG - tig0025908:34650-34669 Msa1425950:three_prime_UTR 25.0%
!! AGAAAATGCCTTAATTTGAA+TGG - tig0025908:34872-34891 Msa1425950:CDS 25.0%
! TCAATGTTCCATTCAAATTA+AGG + tig0025908:34883-34902 None:intergenic 25.0%
! CTTTATTCAAGCATATTCTA+AGG - tig0025908:35126-35145 Msa1425950:intron 25.0%
!! AAGTTCACCTTTTAATACAT+TGG + tig0025908:35221-35240 None:intergenic 25.0%
! TTTACTAATTCTCTTAAGCA+TGG - tig0025908:35297-35316 Msa1425950:intron 25.0%
! GTATAGATTCACATAAATCT+TGG + tig0025908:35539-35558 None:intergenic 25.0%
! CTCAAATTCTAATTTGAAGA+CGG - tig0025908:33881-33900 Msa1425950:three_prime_UTR 25.0%
! CTGATTCAAAAGAATATGTA+TGG + tig0025908:34071-34090 None:intergenic 25.0%
!! TTCTTTTGTTCAGGAAAAAA+AGG - tig0025908:34106-34125 Msa1425950:three_prime_UTR 25.0%
! TTTAGTCACTCATAGTAATA+TGG + tig0025908:34438-34457 None:intergenic 25.0%
! GTAAACTAAGCATAAATCTA+TGG - tig0025908:34481-34500 Msa1425950:intron 25.0%
! CAATATGATTCTTCAATTGA+TGG - tig0025908:34650-34669 Msa1425950:three_prime_UTR 25.0%
!! AGAAAATGCCTTAATTTGAA+TGG - tig0025908:34872-34891 Msa1425950:CDS 25.0%
! TCAATGTTCCATTCAAATTA+AGG + tig0025908:34883-34902 None:intergenic 25.0%
! CTTTATTCAAGCATATTCTA+AGG - tig0025908:35126-35145 Msa1425950:intron 25.0%
!! AAGTTCACCTTTTAATACAT+TGG + tig0025908:35221-35240 None:intergenic 25.0%
! TTTACTAATTCTCTTAAGCA+TGG - tig0025908:35297-35316 Msa1425950:intron 25.0%
! GTATAGATTCACATAAATCT+TGG + tig0025908:35539-35558 None:intergenic 25.0%
!! CATAATTAGAATTTGCTGCA+TGG + tig0025908:33855-33874 None:intergenic 30.0%
CAAATGAAAAGAAATGTGTG+AGG - tig0025908:33971-33990 Msa1425950:three_prime_UTR 30.0%
! GTGATTCATTTCTTTTGTTC+AGG - tig0025908:34097-34116 Msa1425950:three_prime_UTR 30.0%
TCAAAATCTGCACTAAAACA+TGG + tig0025908:34268-34287 None:intergenic 30.0%
CCTTTGTTAATGCTTACAAT+CGG - tig0025908:34366-34385 Msa1425950:intron 30.0%
! GTACTACTAACTTGTGAATT+CGG + tig0025908:34406-34425 None:intergenic 30.0%
! AAATCTATGGTTTTCCTAGT+TGG - tig0025908:34494-34513 Msa1425950:intron 30.0%
CATTGAAAAAATCAGCTTCT+AGG - tig0025908:34726-34745 Msa1425950:CDS 30.0%
! CTTTGTTATAACATGTGTGA+TGG - tig0025908:34799-34818 Msa1425950:CDS 30.0%
ACAGGGATGTAAAAGAATAA+TGG + tig0025908:34839-34858 None:intergenic 30.0%
!! TCAAATTAAGGCATTTTCTC+TGG + tig0025908:34871-34890 None:intergenic 30.0%
TATCTCATTGCTATAGCAAA+AGG + tig0025908:35034-35053 None:intergenic 30.0%
!! TGTAATGAAGTGTTGATAGA+TGG - tig0025908:35420-35439 Msa1425950:intron 30.0%
TATATTAACTGAGACAGCAT+GGG - tig0025908:35589-35608 Msa1425950:intron 30.0%
!! CATAATTAGAATTTGCTGCA+TGG + tig0025908:33855-33874 None:intergenic 30.0%
CAAATGAAAAGAAATGTGTG+AGG - tig0025908:33971-33990 Msa1425950:three_prime_UTR 30.0%
! GTGATTCATTTCTTTTGTTC+AGG - tig0025908:34097-34116 Msa1425950:three_prime_UTR 30.0%
TCAAAATCTGCACTAAAACA+TGG + tig0025908:34268-34287 None:intergenic 30.0%
CCTTTGTTAATGCTTACAAT+CGG - tig0025908:34366-34385 Msa1425950:intron 30.0%
! GTACTACTAACTTGTGAATT+CGG + tig0025908:34406-34425 None:intergenic 30.0%
! AAATCTATGGTTTTCCTAGT+TGG - tig0025908:34494-34513 Msa1425950:intron 30.0%
CATTGAAAAAATCAGCTTCT+AGG - tig0025908:34726-34745 Msa1425950:CDS 30.0%
! CTTTGTTATAACATGTGTGA+TGG - tig0025908:34799-34818 Msa1425950:CDS 30.0%
ACAGGGATGTAAAAGAATAA+TGG + tig0025908:34839-34858 None:intergenic 30.0%
!! TCAAATTAAGGCATTTTCTC+TGG + tig0025908:34871-34890 None:intergenic 30.0%
TATCTCATTGCTATAGCAAA+AGG + tig0025908:35034-35053 None:intergenic 30.0%
!! TGTAATGAAGTGTTGATAGA+TGG - tig0025908:35420-35439 Msa1425950:intron 30.0%
TATATTAACTGAGACAGCAT+GGG - tig0025908:35589-35608 Msa1425950:intron 30.0%
!! AATTTGAAGACGGATTTTGC+AGG - tig0025908:33891-33910 Msa1425950:three_prime_UTR 35.0%
!! ATTTGAAGACGGATTTTGCA+GGG - tig0025908:33892-33911 Msa1425950:three_prime_UTR 35.0%
TGTATGGCAACTGAAACAAT+AGG + tig0025908:34055-34074 None:intergenic 35.0%
GTCATTTGATTGAGATCGAA+CGG - tig0025908:34241-34260 Msa1425950:three_prime_UTR 35.0%
CCGATTGTAAGCATTAACAA+AGG + tig0025908:34369-34388 None:intergenic 35.0%
! TTCTGTCCAGATTTGATGAT+TGG - tig0025908:34597-34616 Msa1425950:three_prime_UTR 35.0%
TGACTTCCAATCATCAAATC+TGG + tig0025908:34606-34625 None:intergenic 35.0%
!! TTGTTCCATTTCTGAAGTAG+TGG + tig0025908:34776-34795 None:intergenic 35.0%
! TTTTACATCCCTGTGATTGA+TGG - tig0025908:34845-34864 Msa1425950:CDS 35.0%
AGATTACTGCTAGTGTTCTT+TGG - tig0025908:34903-34922 Msa1425950:CDS 35.0%
!!! ATCGTCTTTCAGTTTTGAAC+TGG - tig0025908:34956-34975 Msa1425950:CDS 35.0%
!!! TCGTCTTTCAGTTTTGAACT+GGG - tig0025908:34957-34976 Msa1425950:CDS 35.0%
!! AGCATGGTTAAAAGTATCGT+AGG - tig0025908:35313-35332 Msa1425950:intron 35.0%
GTATATTAACTGAGACAGCA+TGG - tig0025908:35588-35607 Msa1425950:intron 35.0%
!! AATTTGAAGACGGATTTTGC+AGG - tig0025908:33891-33910 Msa1425950:three_prime_UTR 35.0%
!! ATTTGAAGACGGATTTTGCA+GGG - tig0025908:33892-33911 Msa1425950:three_prime_UTR 35.0%
TGTATGGCAACTGAAACAAT+AGG + tig0025908:34055-34074 None:intergenic 35.0%
GTCATTTGATTGAGATCGAA+CGG - tig0025908:34241-34260 Msa1425950:three_prime_UTR 35.0%
CCGATTGTAAGCATTAACAA+AGG + tig0025908:34369-34388 None:intergenic 35.0%
! TTCTGTCCAGATTTGATGAT+TGG - tig0025908:34597-34616 Msa1425950:three_prime_UTR 35.0%
TGACTTCCAATCATCAAATC+TGG + tig0025908:34606-34625 None:intergenic 35.0%
!! TTGTTCCATTTCTGAAGTAG+TGG + tig0025908:34776-34795 None:intergenic 35.0%
! TTTTACATCCCTGTGATTGA+TGG - tig0025908:34845-34864 Msa1425950:CDS 35.0%
AGATTACTGCTAGTGTTCTT+TGG - tig0025908:34903-34922 Msa1425950:CDS 35.0%
!!! ATCGTCTTTCAGTTTTGAAC+TGG - tig0025908:34956-34975 Msa1425950:CDS 35.0%
!!! TCGTCTTTCAGTTTTGAACT+GGG - tig0025908:34957-34976 Msa1425950:CDS 35.0%
!! AGCATGGTTAAAAGTATCGT+AGG - tig0025908:35313-35332 Msa1425950:intron 35.0%
GTATATTAACTGAGACAGCA+TGG - tig0025908:35588-35607 Msa1425950:intron 35.0%
AATAGGCAATAGCACATGTG+AGG + tig0025908:34038-34057 None:intergenic 40.0%
ACTACGTGAATGAGTGCAAT+CGG + tig0025908:34208-34227 None:intergenic 40.0%
CATTCACGTAGTCAACACAT+CGG - tig0025908:34216-34235 Msa1425950:three_prime_UTR 40.0%
ACCATCTGATCTCTATCCAA+AGG - tig0025908:34326-34345 Msa1425950:intron 40.0%
ACCTTTGGATAGAGATCAGA+TGG + tig0025908:34330-34349 None:intergenic 40.0%
TTGTGAATTCGGATTCCTTG+TGG + tig0025908:34395-34414 None:intergenic 40.0%
AGTAGTGTTCTTGATCCACA+AGG - tig0025908:34749-34768 Msa1425950:CDS 40.0%
AAGGTCCACTACTTCAGAAA+TGG - tig0025908:34768-34787 Msa1425950:CDS 40.0%
ATGTGTGATGGCAGTATCTA+TGG - tig0025908:34811-34830 Msa1425950:CDS 40.0%
!! CATTTTTGGAAGGAGAGAGT+CGG - tig0025908:35000-35019 Msa1425950:CDS 40.0%
ATCAATTCTTCCACTTCCTC+AGG - tig0025908:35084-35103 Msa1425950:intron 40.0%
!! TATTCTGAAACCTGAGGAAG+TGG + tig0025908:35097-35116 None:intergenic 40.0%
!! GTACTGTATTCTGAAACCTG+AGG + tig0025908:35103-35122 None:intergenic 40.0%
GCTATGATTCCAATTCCACA+AGG - tig0025908:35451-35470 Msa1425950:intron 40.0%
ATATATGGAACTGAGCCTTG+TGG + tig0025908:35469-35488 None:intergenic 40.0%
AATAGGCAATAGCACATGTG+AGG + tig0025908:34038-34057 None:intergenic 40.0%
ACTACGTGAATGAGTGCAAT+CGG + tig0025908:34208-34227 None:intergenic 40.0%
CATTCACGTAGTCAACACAT+CGG - tig0025908:34216-34235 Msa1425950:three_prime_UTR 40.0%
ACCATCTGATCTCTATCCAA+AGG - tig0025908:34326-34345 Msa1425950:intron 40.0%
ACCTTTGGATAGAGATCAGA+TGG + tig0025908:34330-34349 None:intergenic 40.0%
TTGTGAATTCGGATTCCTTG+TGG + tig0025908:34395-34414 None:intergenic 40.0%
AGTAGTGTTCTTGATCCACA+AGG - tig0025908:34749-34768 Msa1425950:CDS 40.0%
AAGGTCCACTACTTCAGAAA+TGG - tig0025908:34768-34787 Msa1425950:CDS 40.0%
ATGTGTGATGGCAGTATCTA+TGG - tig0025908:34811-34830 Msa1425950:CDS 40.0%
!! CATTTTTGGAAGGAGAGAGT+CGG - tig0025908:35000-35019 Msa1425950:CDS 40.0%
ATCAATTCTTCCACTTCCTC+AGG - tig0025908:35084-35103 Msa1425950:intron 40.0%
!! TATTCTGAAACCTGAGGAAG+TGG + tig0025908:35097-35116 None:intergenic 40.0%
!! GTACTGTATTCTGAAACCTG+AGG + tig0025908:35103-35122 None:intergenic 40.0%
GCTATGATTCCAATTCCACA+AGG - tig0025908:35451-35470 Msa1425950:intron 40.0%
ATATATGGAACTGAGCCTTG+TGG + tig0025908:35469-35488 None:intergenic 40.0%
TAACAAAGGCAGCAGCACAT+CGG + tig0025908:34355-34374 None:intergenic 45.0%
!! TTCTGAAGTAGTGGACCTTG+TGG + tig0025908:34767-34786 None:intergenic 45.0%
TTCTCTGGCCATCAATCACA+GGG + tig0025908:34856-34875 None:intergenic 45.0%
TTTCTCTGGCCATCAATCAC+AGG + tig0025908:34857-34876 None:intergenic 45.0%
CTTCCAAAAATGCGCGAAGA+AGG + tig0025908:34992-35011 None:intergenic 45.0%
!! CTTCTTCGCGCATTTTTGGA+AGG - tig0025908:34990-35009 Msa1425950:CDS 45.0%
TAACAAAGGCAGCAGCACAT+CGG + tig0025908:34355-34374 None:intergenic 45.0%
!! TTCTGAAGTAGTGGACCTTG+TGG + tig0025908:34767-34786 None:intergenic 45.0%
TTCTCTGGCCATCAATCACA+GGG + tig0025908:34856-34875 None:intergenic 45.0%
TTTCTCTGGCCATCAATCAC+AGG + tig0025908:34857-34876 None:intergenic 45.0%
CTTCCAAAAATGCGCGAAGA+AGG + tig0025908:34992-35011 None:intergenic 45.0%
!! CTTCTTCGCGCATTTTTGGA+AGG - tig0025908:34990-35009 Msa1425950:CDS 45.0%
TCACGTAGTCAACACATCGG+TGG - tig0025908:34219-34238 Msa1425950:three_prime_UTR 50.0%
GCTTACAATCGGTGACCACA+AGG - tig0025908:34377-34396 Msa1425950:intron 50.0%
! GCACCTTCTTCGCGCATTTT+TGG - tig0025908:34986-35005 Msa1425950:CDS 50.0%
! GGAACTGAGCCTTGTGGAAT+TGG + tig0025908:35463-35482 None:intergenic 50.0%
TTAACTGAGACAGCATGGGC+TGG - tig0025908:35593-35612 Msa1425950:intron 50.0%
TCACGTAGTCAACACATCGG+TGG - tig0025908:34219-34238 Msa1425950:three_prime_UTR 50.0%
GCTTACAATCGGTGACCACA+AGG - tig0025908:34377-34396 Msa1425950:intron 50.0%
! GCACCTTCTTCGCGCATTTT+TGG - tig0025908:34986-35005 Msa1425950:CDS 50.0%
! GGAACTGAGCCTTGTGGAAT+TGG + tig0025908:35463-35482 None:intergenic 50.0%
TTAACTGAGACAGCATGGGC+TGG - tig0025908:35593-35612 Msa1425950:intron 50.0%
TGAGTGCAATCGGTGACTGC+AGG + tig0025908:34198-34217 None:intergenic 55.0%
AGCAGCACATCGGTGACCTT+TGG + tig0025908:34345-34364 None:intergenic 55.0%
TGAGTGCAATCGGTGACTGC+AGG + tig0025908:34198-34217 None:intergenic 55.0%
AGCAGCACATCGGTGACCTT+TGG + tig0025908:34345-34364 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0025908 gene 33801 35898 33801 ID=Msa1425950;Name=Msa1425950
tig0025908 mRNA 33801 35898 33801 ID=Msa1425950-mRNA-1;Parent=Msa1425950;Name=Msa1425950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=163|1|1|1|0.5|0.33|3|592|131
tig0025908 exon 33801 34260 33801 ID=Msa1425950-mRNA-1:exon:4588;Parent=Msa1425950-mRNA-1
tig0025908 exon 34594 35092 34594 ID=Msa1425950-mRNA-1:exon:4587;Parent=Msa1425950-mRNA-1
tig0025908 exon 35707 35898 35707 ID=Msa1425950-mRNA-1:exon:4586;Parent=Msa1425950-mRNA-1
tig0025908 five_prime_UTR 35736 35898 35736 ID=Msa1425950-mRNA-1:five_prime_utr;Parent=Msa1425950-mRNA-1
tig0025908 CDS 35707 35735 35707 ID=Msa1425950-mRNA-1:cds;Parent=Msa1425950-mRNA-1
tig0025908 CDS 34726 35092 34726 ID=Msa1425950-mRNA-1:cds;Parent=Msa1425950-mRNA-1
tig0025908 three_prime_UTR 34594 34725 34594 ID=Msa1425950-mRNA-1:three_prime_utr;Parent=Msa1425950-mRNA-1
tig0025908 three_prime_UTR 33801 34260 33801 ID=Msa1425950-mRNA-1:three_prime_utr;Parent=Msa1425950-mRNA-1
Gene Sequence

>Msa1425950

ATGAGTTCAAATGAAAAGAAATGTGTGAGATTTGATGATTGGAAGTCAGAATCATCATCTTTTAATTTTTCACAATATGATTCTTCAATTGATGGATTTGAAATAAGAAATTCTAAAGCAAGTGTTACTCCAGCAAAAAATAATGAATCATTGAAAAAATCAGCTTCTAGGAGTAGTGTTCTTGATCCACAAGGTCCACTACTTCAGAAATGGAACAAGATCTTTGTTATAACATGTGTGATGGCAGTATCTATGGATCCATTATTCTTTTACATCCCTGTGATTGATGGCCAGAGAAAATGCCTTAATTTGAATGGAACATTGAAGATTACTGCTAGTGTTCTTTGGACGTTTTTCGATCTTTTCTACATTATTCGCATCGTCTTTCAGTTTTGA
Protein sequence

>Msa1425950

MSSNEKKCVRFDDWKSESSSFNFSQYDSSIDGFEIRNSKASVTPAKNNESLKKSASRSSVLDPQGPLLQKWNKIFVITCVMAVSMDPLFFYIPVIDGQRKCLNLNGTLKITASVLWTFFDLFYIIRIVFQF*