AlfalfaGEDB | Msa1431860

M. sativa cultivar zhongmu-4 / Msa1431860

KEGG: -
GO: -
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431860	XP_013448091.1	86.255	502	29	2	1	463	1	501	0.0	853

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431860	sp\|Q6NQK2\|NAC8_ARATH	37.313	469	244	13	2	453	3	438	3.30e-81	261

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431860	A0A072TZ22	86.255	502	29	2	1	463	1	501	0.0	853

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TFs/TRs:

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Gene ID	Type	Classification
Msa1431860	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1014110	Msa1431860	0.980054	2.431800e-149	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431860	MtrunA17_Chr7g0227071	86.255	502	29	2	1	463	1	501	0.0	853
Msa1431860	MtrunA17_Chr5g0422431	38.351	485	212	14	5	456	6	436	2.86e-92	286
Msa1431860	MtrunA17_Chr1g0198751	51.362	257	118	3	5	256	8	262	4.90e-88	277
Msa1431860	MtrunA17_Chr2g0282871	56.216	185	74	3	35	215	31	212	4.80e-67	217
Msa1431860	MtrunA17_Chr4g0046431	55.978	184	74	3	36	215	47	227	1.69e-64	211
Msa1431860	MtrunA17_Chr8g0367761	56.667	180	71	3	36	211	47	223	5.07e-63	205
Msa1431860	MtrunA17_Chr4g0040541	54.054	185	82	1	34	215	24	208	6.39e-63	205
Msa1431860	MtrunA17_Chr8g0389661	53.646	192	80	4	30	215	25	213	8.95e-61	206
Msa1431860	MtrunA17_Chr2g0324961	50.246	203	97	2	34	233	25	226	1.60e-56	189
Msa1431860	MtrunA17_Chr1g0198741	48.227	141	68	2	113	249	10	149	1.42e-35	133

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1431860	AT3G01600.1	55.856	222	97	1	1	222	1	221	6.72e-85	265
Msa1431860	AT5G14490.4	46.897	290	146	3	1	285	1	287	1.35e-83	261
Msa1431860	AT5G14490.3	46.552	290	147	3	1	285	4	290	6.84e-83	260
Msa1431860	AT5G14490.2	51.867	241	113	2	1	241	1	238	7.05e-83	257
Msa1431860	AT5G14490.1	46.552	290	147	3	1	285	4	290	8.84e-83	259
Msa1431860	AT1G25580.1	37.313	469	244	13	2	453	3	438	3.35e-82	261
Msa1431860	AT4G29230.3	52.332	193	82	3	30	215	26	215	1.08e-62	210
Msa1431860	AT4G29230.1	52.332	193	82	3	30	215	26	215	3.18e-62	210
Msa1431860	AT4G29230.2	52.332	193	81	3	30	215	26	214	3.60e-62	210
Msa1431860	AT1G28470.1	49.515	206	91	4	36	233	53	253	3.31e-61	202

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Find 118 sgRNAs with CRISPR-Local

Find 294 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACATACAACTTTGCA+TGG	0.525483	tig0026915:+34887	Msa1431860:CDS
AAAACGCTTCTGGTACAAAA+AGG	0.355639	tig0026915:-35526	None:intergenic
AAAATGCATCAGTACCATCT+AGG	0.444254	tig0026915:+34124	Msa1431860:CDS
AAAATTTGATCCTTCTGATG+TGG	0.522815	tig0026915:+32663	Msa1431860:CDS
AAAGCCTCACATGTTTATCG+AGG	0.708264	tig0026915:+32729	Msa1431860:CDS
AAATCTCGTCACACAATAAT+AGG	0.320076	tig0026915:-35170	None:intergenic
AAATGCATCAGTACCATCTA+GGG	0.560385	tig0026915:+34125	Msa1431860:CDS
AAATTCGGAATATGCCGGCT+TGG	0.543783	tig0026915:+35143	Msa1431860:CDS
AACAACTCTGCATGGTTGGC+TGG	0.431062	tig0026915:+35102	Msa1431860:CDS
AACCACGATGATGAATCTTT+CGG	0.450635	tig0026915:-33961	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTGAACTATCCAGTTTCT+CGG	+	tig0026915:31436-31455	Msa1431860:intron	30.0%
ATTTACAATTTGGGGGTTTA+TGG	+	tig0026915:31629-31648	Msa1431860:intron	30.0%
TTTACAATTTGGGGGTTTAT+GGG	+	tig0026915:31630-31649	Msa1431860:intron	30.0%
TCATCATCAATTGCAGAAAA+TGG	+	tig0026915:31720-31739	Msa1431860:intron	30.0%
AATATACCATTCATATGTGG+TGG	-	tig0026915:31771-31790	None:intergenic	30.0%
AGAAAACCACCAAAATACAA+GGG	-	tig0026915:31936-31955	None:intergenic	30.0%
TAGAAAACCACCAAAATACA+AGG	-	tig0026915:31937-31956	None:intergenic	30.0%
GAATGGCTAATTAACATAAG+AGG	+	tig0026915:32185-32204	Msa1431860:CDS	30.0%
AAAATGCAGGGTATAGTAAT+TGG	-	tig0026915:32461-32480	None:intergenic	30.0%
TCCAACAAAAGAAAAATGCA+GGG	-	tig0026915:32473-32492	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0026915	gene	31084	35816	31084	ID=Msa1431860;Name=Msa1431860
tig0026915	mRNA	31084	35816	31084	ID=Msa1431860-mRNA-1;Parent=Msa1431860;Name=Msa1431860-mRNA-1;_AED=0.04;_eAED=0.04;_QI=119\|1\|0.83\|1\|1\|1\|6\|311\|463
tig0026915	exon	31084	31210	31084	ID=Msa1431860-mRNA-1:exon:7765;Parent=Msa1431860-mRNA-1
tig0026915	exon	32184	32316	32184	ID=Msa1431860-mRNA-1:exon:7766;Parent=Msa1431860-mRNA-1
tig0026915	exon	32628	32814	32628	ID=Msa1431860-mRNA-1:exon:7767;Parent=Msa1431860-mRNA-1
tig0026915	exon	33885	34366	33885	ID=Msa1431860-mRNA-1:exon:7768;Parent=Msa1431860-mRNA-1
tig0026915	exon	34829	35353	34829	ID=Msa1431860-mRNA-1:exon:7769;Parent=Msa1431860-mRNA-1
tig0026915	exon	35449	35816	35449	ID=Msa1431860-mRNA-1:exon:7770;Parent=Msa1431860-mRNA-1
tig0026915	five_prime_UTR	31084	31202	31084	ID=Msa1431860-mRNA-1:five_prime_utr;Parent=Msa1431860-mRNA-1
tig0026915	CDS	31203	31210	31203	ID=Msa1431860-mRNA-1:cds;Parent=Msa1431860-mRNA-1
tig0026915	CDS	32184	32316	32184	ID=Msa1431860-mRNA-1:cds;Parent=Msa1431860-mRNA-1
tig0026915	CDS	32628	32814	32628	ID=Msa1431860-mRNA-1:cds;Parent=Msa1431860-mRNA-1
tig0026915	CDS	33885	34366	33885	ID=Msa1431860-mRNA-1:cds;Parent=Msa1431860-mRNA-1
tig0026915	CDS	34829	35353	34829	ID=Msa1431860-mRNA-1:cds;Parent=Msa1431860-mRNA-1
tig0026915	CDS	35449	35505	35449	ID=Msa1431860-mRNA-1:cds;Parent=Msa1431860-mRNA-1
tig0026915	three_prime_UTR	35506	35816	35506	ID=Msa1431860-mRNA-1:three_prime_utr;Parent=Msa1431860-mRNA-1

Gene Sequence

>Msa1431860

ATGGCTAGGGAATGGCTAATTAACATAAGAGGATTTGCAAAGAAATTGAGAAGCACTTCACTCTCTTCAGCTGATCAGATCAAGGATTGTGGCGCATGCCGTGAATGTCCAAACTGTCATTGTCGTATAGACAACAGTGATGTTTCTAGTGAGTGGCCTGGCTTTCCCCTCGGCATAAAATTTGATCCTTCTGATGTGGAAATATTGGAGCATTTAGCGGCTAAATGCGGTGTTGGGAACACAAAGCCTCACATGTTTATCGAGGAGTTCATACCAACATTGGAAGGAGAACAAGGCATTTGCTATACACATCCTGAAAATCTTCCAGGTGCTAAGAAAGATGGGAGCAGTGTTCATTTCTTTCACAGAACAATGAATGCATATAGTTCTGGTCAACGAAAGCGCCGAAAGATTCATCATCGTGGTTCAACTGAAGACCATGTACGCTGGCACAAGACTGGTAAGACCAAAGCTATAATAGAACATGGAGAACATAAGGGATTTAAGAAGATCATGGTTCTGTATATAAGATCTGAGAAAGAGTCCAAGTCCTACAAATCTGACTGGAAAATGCATCAGTACCATCTAGGGACTGATGAAGACGAGAAGAATGGAGAATACGTCGTTTCAAAAATATTTTATAAGCAGAATGAGAAAAATGAAGATAAAGCAATGGCTGAAGAACCTGACAGTATAACATTGCGAACAAGTCCAAGGACTCCAAACCCAAATCCTCCAAATCCACCGCGTACAGGAAAATGTGTTGACAATGGCAACATCAATGAAACTTCACTGTTGCCATTTGCAGAGGATGTCAAGTCTATCCCTGTAGAATCAAATGCCATGCAATCTGATATTCAGGATCAGGAAAACACATACAACTTTGCATGGTTGGCTGGTGAATCACAGGCTGCCCCGCAATTTGATATTCAGGATCAGGACAACACATTCAACTCTGCATGGTTGGCTGGTGAATCACAGGCTGCCCTGCAATCTGATATTCAAGATCACGACAACTCTGCATGGTTGGCCGGTGAATCGCAAGCTGCCCTGCAAACTGATATTCAGAATCAGGATAACACAAACAACTCTGCATGGTTGGCTGGTGAATCACAGGCTATGGAAAATTCGGAATATGCCGGCTTGGATGACCTATTATTGTGTGACGAGATTTTTGATTCATCTGCTCTATTTACTGATTCTCAATTGGACTCGATCAATGACGTCACTCACTACGAAAACAAAATGACAGGAAATTATAATGTATCTAGTGAAACTTCAACTGCTGTCCTGGATACACTGGAATTGGATACTCCTCCAGAGTATGATCTTTCGAATTTGACATTTGGTTCTCAAGACGACAGTATCCTTGGTTTGATAGGTGGGCTATGA

Protein sequence

>Msa1431860

MAREWLINIRGFAKKLRSTSLSSADQIKDCGACRECPNCHCRIDNSDVSSEWPGFPLGIKFDPSDVEILEHLAAKCGVGNTKPHMFIEEFIPTLEGEQGICYTHPENLPGAKKDGSSVHFFHRTMNAYSSGQRKRRKIHHRGSTEDHVRWHKTGKTKAIIEHGEHKGFKKIMVLYIRSEKESKSYKSDWKMHQYHLGTDEDEKNGEYVVSKIFYKQNEKNEDKAMAEEPDSITLRTSPRTPNPNPPNPPRTGKCVDNGNINETSLLPFAEDVKSIPVESNAMQSDIQDQENTYNFAWLAGESQAAPQFDIQDQDNTFNSAWLAGESQAALQSDIQDHDNSAWLAGESQAALQTDIQNQDNTNNSAWLAGESQAMENSEYAGLDDLLLCDEIFDSSALFTDSQLDSINDVTHYENKMTGNYNVSSETSTAVLDTLELDTPPEYDLSNLTFGSQDDSILGLIGGL*