AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1432910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432910 MtrunA17_Chr8g0389241 92.883 281 12 4 1 274 1 280 3.15e-170 471
Msa1432910 MtrunA17_Chr6g0488471 52.145 303 102 13 1 274 1 289 7.09e-75 230
Msa1432910 MtrunA17_Chr2g0331031 53.371 178 65 5 4 169 3 174 1.56e-46 159
Msa1432910 MtrunA17_Chr4g0055931 47.283 184 67 6 4 176 3 167 1.03e-43 151
Msa1432910 MtrunA17_Chr5g0415191 45.143 175 73 5 4 168 5 166 4.12e-38 136
Msa1432910 MtrunA17_Chr4g0062741 67.089 79 25 1 99 176 132 210 3.79e-33 122
Msa1432910 MtrunA17_Chr3g0135671 35.741 263 128 8 4 260 5 232 7.97e-32 117
Msa1432910 MtrunA17_Chr1g0162081 65.823 79 26 1 99 176 116 194 8.00e-32 119
Msa1432910 MtrunA17_Chr7g0241761 76.056 71 16 1 99 168 134 204 7.84e-31 117
Msa1432910 MtrunA17_Chr3g0104071 71.233 73 20 1 97 168 139 211 2.23e-30 115
Msa1432910 MtrunA17_Chr5g0429521 70.270 74 21 1 96 168 123 196 2.39e-30 115
Msa1432910 MtrunA17_Chr1g0210471 62.791 86 31 1 84 168 103 188 8.90e-30 112
Msa1432910 MtrunA17_Chr5g0436501 64.474 76 24 1 96 168 116 191 4.52e-27 105
Msa1432910 MtrunA17_Chr4g0059701 79.245 53 11 0 99 151 78 130 7.43e-27 105
Msa1432910 MtrunA17_Chr3g0092621 62.319 69 25 1 105 172 84 152 1.06e-25 99.8
Msa1432910 MtrunA17_Chr6g0452791 75.000 48 12 0 105 152 80 127 2.00e-21 88.6
Msa1432910 MtrunA17_Chr5g0409841 52.632 76 33 2 101 173 88 163 9.10e-19 82.8
Msa1432910 MtrunA17_Chr5g0440731 49.383 81 37 2 97 173 97 177 9.30e-19 84.0
Msa1432910 MtrunA17_Chr5g0440741 54.930 71 28 2 107 173 102 172 6.81e-18 81.6
Msa1432910 MtrunA17_Chr5g0409831 57.143 56 24 0 101 156 73 128 3.93e-16 75.5
Msa1432910 MtrunA17_Chr8g0365011 46.341 82 29 2 107 173 94 175 2.90e-14 71.2
Msa1432910 MtrunA17_Chr8g0364731 56.452 62 24 1 115 173 11 72 1.11e-13 67.8
Msa1432910 MtrunA17_Chr1g0192061 51.562 64 26 3 107 166 63 125 1.02e-12 65.1
Msa1432910 MtrunA17_Chr5g0432921 63.830 47 16 1 107 153 115 160 9.74e-12 63.2
Msa1432910 MtrunA17_Chr5g0432941 61.702 47 17 1 107 153 115 160 3.04e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432910 AT5G56840.1 54.891 184 51 5 1 174 1 162 5.82e-53 172
Msa1432910 AT5G61620.1 47.904 167 81 3 4 168 10 172 1.94e-44 153
Msa1432910 AT5G47390.1 43.062 209 96 5 4 208 3 192 1.10e-43 152
Msa1432910 AT1G70000.1 47.514 181 60 6 4 169 3 163 5.69e-40 140
Msa1432910 AT1G70000.2 47.514 181 60 6 4 169 3 163 5.69e-40 140
Msa1432910 AT3G16350.1 44.286 210 63 7 4 169 3 202 3.96e-36 133
Msa1432910 AT1G49010.1 69.737 76 22 1 99 173 128 203 1.44e-32 122
Msa1432910 AT5G08520.1 63.855 83 27 2 99 178 112 194 1.79e-31 118
Msa1432910 AT5G01200.1 68.293 82 23 2 90 168 129 210 4.99e-31 116
Msa1432910 AT2G38090.1 73.239 71 18 1 99 168 135 205 5.73e-30 114
Msa1432910 AT5G58900.1 69.863 73 21 1 97 168 133 205 1.90e-29 113
Msa1432910 AT3G11280.2 54.128 109 43 3 96 199 118 224 3.10e-28 109
Msa1432910 AT3G11280.1 54.128 109 43 3 96 199 118 224 3.10e-28 109
Msa1432910 AT5G04760.1 65.714 70 23 1 100 168 94 163 4.65e-27 105
Msa1432910 AT5G05790.2 67.568 74 23 1 96 168 122 195 5.25e-27 106
Msa1432910 AT5G05790.1 67.568 74 23 1 96 168 122 195 5.25e-27 106
Msa1432910 AT1G19000.2 80.392 51 10 0 101 151 98 148 2.70e-25 102
Msa1432910 AT1G19000.1 80.392 51 10 0 101 151 98 148 2.70e-25 102
Msa1432910 AT1G74840.2 76.923 52 12 0 100 151 91 142 1.86e-24 98.6
Msa1432910 AT1G74840.1 76.923 52 12 0 100 151 91 142 2.49e-24 99.0
Msa1432910 AT3G10580.2 61.765 68 25 1 101 168 91 157 2.03e-21 90.9
Msa1432910 AT3G10580.1 61.765 68 25 1 101 168 91 157 2.70e-21 91.3
Msa1432910 AT5G23650.1 56.579 76 32 1 99 173 114 189 2.91e-21 92.0
Msa1432910 AT4G09450.1 67.925 53 17 0 101 153 87 139 6.88e-18 80.1
Msa1432910 AT3G10590.1 54.930 71 28 3 101 168 105 174 1.45e-14 71.2

Find 74 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAATTAAGTGACGCTAAT+TGG 0.117960 tig0027185:+14549 None:intergenic
CCTGCTGGCTCATTCCTTCT+TGG 0.142813 tig0027185:-14573 Msa1432910:CDS
TGCTAATTCAGTAGTAAATT+TGG 0.198359 tig0027185:-14814 Msa1432910:CDS
TTGGAGGACTTCGTCTCTTT+GGG 0.209128 tig0027185:-16068 Msa1432910:CDS
CTTGGAGGACTTCGTCTCTT+TGG 0.217697 tig0027185:-16069 Msa1432910:CDS
TTGTGCAGCAGTGCCTAATT+TGG 0.253498 tig0027185:-14646 Msa1432910:CDS
ATAATTACTCTACAGATAAT+TGG 0.274738 tig0027185:-14686 Msa1432910:CDS
TATACCTTTCTTTCTCTCTT+GGG 0.282804 tig0027185:+15849 None:intergenic
TTTAATTGGTGGTTTGTAAT+GGG 0.303409 tig0027185:-16159 Msa1432910:exon
ATATACCTTTCTTTCTCTCT+TGG 0.345963 tig0027185:+15848 None:intergenic
GAAAGTGTGAGGTCCAAATT+AGG 0.360682 tig0027185:+14633 None:intergenic
CTTTAATTGGTGGTTTGTAA+TGG 0.382037 tig0027185:-16160 Msa1432910:exon
TTAATTAAGTGACGCTAATT+GGG 0.384534 tig0027185:+14550 None:intergenic
GAACAAACGAATTAGTTTAG+AGG 0.384852 tig0027185:+16229 None:intergenic
CTGTGCTCTTCTTCTGTCCA+TGG 0.393875 tig0027185:+15334 None:intergenic
GCTAATTCAGTAGTAAATTT+GGG 0.397356 tig0027185:-14813 Msa1432910:CDS
GAGCACAGAACATTCCTTGT+TGG 0.426067 tig0027185:-15319 Msa1432910:CDS
TGTTCGCATTGTGGTAAGAT+AGG 0.440998 tig0027185:-16126 Msa1432910:CDS
AATGAGGAAGATGGAGAGGA+TGG 0.441387 tig0027185:+15967 None:intergenic
CATGGCTAGCGACTTGTGTA+GGG 0.443009 tig0027185:+15233 None:intergenic
ATCTTCTTGTACATGAGGTT+AGG 0.445120 tig0027185:+14452 None:intergenic
GCATGGCTAGCGACTTGTGT+AGG 0.465972 tig0027185:+15232 None:intergenic
ATACCTTTCTTTCTCTCTTG+GGG 0.468820 tig0027185:+15850 None:intergenic
CCAAGAAGGAATGAGCCAGC+AGG 0.469903 tig0027185:+14573 None:intergenic
CACACACACACTTTAATTGG+TGG 0.471799 tig0027185:-16170 Msa1432910:five_prime_UTR
CAAAATCAACCCTCCCCTGC+TGG 0.476253 tig0027185:-14588 Msa1432910:CDS
GGATTAGAGAAGCTAGGAAA+AGG 0.476740 tig0027185:-15298 Msa1432910:CDS
TGCACATCCTTCACTACCCT+TGG 0.478767 tig0027185:-16087 Msa1432910:CDS
AGAAGCTAGGAAAAGGAGAC+TGG 0.482282 tig0027185:-15291 Msa1432910:CDS
CCTTTCTTTCTCTCTTGGGG+TGG 0.484527 tig0027185:+15853 None:intergenic
TGGAGAGGATGGTGAGGGAA+AGG 0.486578 tig0027185:+15978 None:intergenic
CAAGAAGGAATGAGCCAGCA+GGG 0.487978 tig0027185:+14574 None:intergenic
ACTTAATTAATTAAGTACTC+TGG 0.497298 tig0027185:-14537 Msa1432910:exon
TGCTTCTTCTTGTTTAAAGT+AGG 0.502048 tig0027185:+14731 None:intergenic
GTAAGATAGGACATAATTGT+AGG 0.502669 tig0027185:-16113 Msa1432910:CDS
CCACCCCAAGAGAGAAAGAA+AGG 0.504684 tig0027185:-15853 Msa1432910:intron
AGAGAATGAGGAAGATGGAG+AGG 0.510998 tig0027185:+15963 None:intergenic
ATCATATCTTCTTGTACATG+AGG 0.512407 tig0027185:+14447 None:intergenic
GTACAAGAAGATATGATCAA+TGG 0.515773 tig0027185:-14441 Msa1432910:three_prime_UTR
AAGGAATGAGCCAGCAGGGG+AGG 0.519594 tig0027185:+14578 None:intergenic
GATGAAGAGAATGAGGAAGA+TGG 0.523435 tig0027185:+15958 None:intergenic
AAATTTGGGTCATAAATTTG+AGG 0.527043 tig0027185:-14799 Msa1432910:CDS
ACGAGGAGGATGATGATAGT+TGG 0.527221 tig0027185:+16043 None:intergenic
CTTGTTGGATTAGAGAAGCT+AGG 0.528666 tig0027185:-15304 Msa1432910:CDS
TTGATTGATCAGGAGTTCCA+TGG 0.532260 tig0027185:-15351 Msa1432910:intron
ACATCCTTCACTACCCTTGG+AGG 0.544075 tig0027185:-16084 Msa1432910:CDS
TGACGCTAATTGGGCCAAGA+AGG 0.545277 tig0027185:+14559 None:intergenic
AGGAATGAGCCAGCAGGGGA+GGG 0.549931 tig0027185:+14579 None:intergenic
GGAAGATGGAGAGGATGGTG+AGG 0.553718 tig0027185:+15972 None:intergenic
TTAATTGGTGGTTTGTAATG+GGG 0.554717 tig0027185:-16158 Msa1432910:exon
TGTGTGTGTGAGAATGAGAA+TGG 0.555374 tig0027185:+16189 None:intergenic
AGATATGAAATATTAGAAGT+GGG 0.561423 tig0027185:+15895 None:intergenic
GATCAAGAAAAGCTTTAGCA+TGG 0.562627 tig0027185:-16004 Msa1432910:CDS
TAGATATGAAATATTAGAAG+TGG 0.562815 tig0027185:+15894 None:intergenic
GAAGATGGAGAGGATGGTGA+GGG 0.564987 tig0027185:+15973 None:intergenic
TGGAGGACTTCGTCTCTTTG+GGG 0.565207 tig0027185:-16067 Msa1432910:CDS
AACACCAACACAAAAGAACA+AGG 0.589703 tig0027185:-14840 Msa1432910:CDS
CAAAGAGACGAAGTCCTCCA+AGG 0.592483 tig0027185:+16070 None:intergenic
TGATGTTGATGAAGAGAATG+AGG 0.596418 tig0027185:+15951 None:intergenic
CTAGCTTCTCTAATCCAACA+AGG 0.600496 tig0027185:+15305 None:intergenic
AGAAGAAAGTGTTCGCATTG+TGG 0.603130 tig0027185:-16135 Msa1432910:CDS
AAGTCCTCCAAGGGTAGTGA+AGG 0.604978 tig0027185:+16080 None:intergenic
ACGTTCAAGTCTCTTCGACT+TGG 0.607278 tig0027185:-15167 Msa1432910:intron
TTGTTTAAAGTAGGACAAAG+TGG 0.607562 tig0027185:+14740 None:intergenic
TGTGAAGTTGAGATGAATGA+TGG 0.607811 tig0027185:-14765 Msa1432910:CDS
GTGTGTGTGAGAATGAGAAT+GGG 0.611563 tig0027185:+16190 None:intergenic
AAGAAGGAATGAGCCAGCAG+GGG 0.613479 tig0027185:+14575 None:intergenic
CATGGTACTAGATGATGACG+AGG 0.629229 tig0027185:+16026 None:intergenic
AAAGAGACGAAGTCCTCCAA+GGG 0.643343 tig0027185:+16071 None:intergenic
ATGGCTAGCGACTTGTGTAG+GGG 0.659705 tig0027185:+15234 None:intergenic
CTAGGAAAAGGAGACTGGAG+AGG 0.661686 tig0027185:-15286 Msa1432910:CDS
GGGATGCAACTGAAAGTGTG+AGG 0.693635 tig0027185:+14622 None:intergenic
GGTACTAGATGATGACGAGG+AGG 0.697526 tig0027185:+16029 None:intergenic
ACACCAACACAAAAGAACAA+GGG 0.712802 tig0027185:-14839 Msa1432910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATAAAATATTTATTT+AGG + tig0027185:15736-15755 None:intergenic 0.0%
!!! TTTTATTATTTTTATTTTTA+TGG - tig0027185:15745-15764 Msa1432910:intron 0.0%
!! AAATAATAAAATATTTATTT+AGG + tig0027185:15736-15755 None:intergenic 0.0%
!!! TTTTATTATTTTTATTTTTA+TGG - tig0027185:15745-15764 Msa1432910:intron 0.0%
!!! ATAATAATTTTCATCAATAT+TGG + tig0027185:14741-14760 None:intergenic 10.0%
!! ATAAATTAAAAAACAAATGA+AGG + tig0027185:15053-15072 None:intergenic 10.0%
!! AAATAAAAAAAAGTTAAAAG+AGG + tig0027185:15691-15710 None:intergenic 10.0%
!!! ATAATAATTTTCATCAATAT+TGG + tig0027185:14741-14760 None:intergenic 10.0%
!! ATAAATTAAAAAACAAATGA+AGG + tig0027185:15053-15072 None:intergenic 10.0%
!! AAATAAAAAAAAGTTAAAAG+AGG + tig0027185:15691-15710 None:intergenic 10.0%
!! TATATTTATACATATGTTAG+CGG - tig0027185:14832-14851 Msa1432910:CDS 15.0%
!!! TATAAAGGAATATAAGATTT+TGG + tig0027185:15137-15156 None:intergenic 15.0%
!! TCTTATATTCCTTTATATAT+TGG - tig0027185:15140-15159 Msa1432910:intron 15.0%
!!! TAATTTTTATTTGTGGTTAT+GGG + tig0027185:15640-15659 None:intergenic 15.0%
!!! ATAATTTTTATTTGTGGTTA+TGG + tig0027185:15641-15660 None:intergenic 15.0%
!! ATATTATTAATTCAATGACA+AGG + tig0027185:15773-15792 None:intergenic 15.0%
!! TATATTTATACATATGTTAG+CGG - tig0027185:14832-14851 Msa1432910:CDS 15.0%
!!! TATAAAGGAATATAAGATTT+TGG + tig0027185:15137-15156 None:intergenic 15.0%
!! TCTTATATTCCTTTATATAT+TGG - tig0027185:15140-15159 Msa1432910:intron 15.0%
!!! TAATTTTTATTTGTGGTTAT+GGG + tig0027185:15640-15659 None:intergenic 15.0%
!!! ATAATTTTTATTTGTGGTTA+TGG + tig0027185:15641-15660 None:intergenic 15.0%
!! ATATTATTAATTCAATGACA+AGG + tig0027185:15773-15792 None:intergenic 15.0%
!! AGATATGAAATATTAGAAGT+GGG + tig0027185:14770-14789 None:intergenic 20.0%
!! TAGATATGAAATATTAGAAG+TGG + tig0027185:14771-14790 None:intergenic 20.0%
!!! TATCTATCAGATTGTTTTAT+AGG - tig0027185:14785-14804 Msa1432910:CDS 20.0%
!! TCTTTCAATTCTATTCTTAT+TGG + tig0027185:15103-15122 None:intergenic 20.0%
!! TATTCCTTTATATATTGGTA+TGG - tig0027185:15145-15164 Msa1432910:intron 20.0%
!! TCAAAAATACTTTCTTAGAT+TGG - tig0027185:15453-15472 Msa1432910:intron 20.0%
!! AATATAAAGTAAGTCTTACA+TGG - tig0027185:15607-15626 Msa1432910:intron 20.0%
!! ATAATTACTCTACAGATAAT+TGG - tig0027185:15976-15995 Msa1432910:CDS 20.0%
!! ACTTAATTAATTAAGTACTC+TGG - tig0027185:16125-16144 Msa1432910:CDS 20.0%
!! AGATATGAAATATTAGAAGT+GGG + tig0027185:14770-14789 None:intergenic 20.0%
!! TAGATATGAAATATTAGAAG+TGG + tig0027185:14771-14790 None:intergenic 20.0%
!!! TATCTATCAGATTGTTTTAT+AGG - tig0027185:14785-14804 Msa1432910:CDS 20.0%
!! TCTTTCAATTCTATTCTTAT+TGG + tig0027185:15103-15122 None:intergenic 20.0%
!! TATTCCTTTATATATTGGTA+TGG - tig0027185:15145-15164 Msa1432910:intron 20.0%
!! TCAAAAATACTTTCTTAGAT+TGG - tig0027185:15453-15472 Msa1432910:intron 20.0%
!! AATATAAAGTAAGTCTTACA+TGG - tig0027185:15607-15626 Msa1432910:intron 20.0%
!! ATAATTACTCTACAGATAAT+TGG - tig0027185:15976-15995 Msa1432910:CDS 20.0%
!! ACTTAATTAATTAAGTACTC+TGG - tig0027185:16125-16144 Msa1432910:CDS 20.0%
!! TTTAATTGGTGGTTTGTAAT+GGG - tig0027185:14503-14522 Msa1432910:three_prime_UTR 25.0%
! ACATCCATACCAATATATAA+AGG + tig0027185:15152-15171 None:intergenic 25.0%
! TATACTTCTAATTTGAGCTA+AGG - tig0027185:15209-15228 Msa1432910:CDS 25.0%
! ATACTTCTAATTTGAGCTAA+GGG - tig0027185:15210-15229 Msa1432910:CDS 25.0%
! ATGATGAATGAGAAAATACT+TGG - tig0027185:15257-15276 Msa1432910:CDS 25.0%
! TGATGAATGAGAAAATACTT+GGG - tig0027185:15258-15277 Msa1432910:CDS 25.0%
!! TATATACTGATTGATTGATC+AGG - tig0027185:15301-15320 Msa1432910:CDS 25.0%
!!! TAAGAAAGTATTTTTGAGCA+TGG + tig0027185:15450-15469 None:intergenic 25.0%
! AAGACTTACTTTATATTCAG+TGG + tig0027185:15604-15623 None:intergenic 25.0%
! TGCTAATTCAGTAGTAAATT+TGG - tig0027185:15848-15867 Msa1432910:intron 25.0%
! GCTAATTCAGTAGTAAATTT+GGG - tig0027185:15849-15868 Msa1432910:intron 25.0%
! AAATTTGGGTCATAAATTTG+AGG - tig0027185:15863-15882 Msa1432910:CDS 25.0%
!!! ATTTTCTTGCTTTTCTGATT+TGG + tig0027185:15958-15977 None:intergenic 25.0%
! TTAATTAAGTGACGCTAATT+GGG + tig0027185:16115-16134 None:intergenic 25.0%
! ATTAATTAAGTGACGCTAAT+TGG + tig0027185:16116-16135 None:intergenic 25.0%
!! TTTAATTGGTGGTTTGTAAT+GGG - tig0027185:14503-14522 Msa1432910:three_prime_UTR 25.0%
! ACATCCATACCAATATATAA+AGG + tig0027185:15152-15171 None:intergenic 25.0%
! TATACTTCTAATTTGAGCTA+AGG - tig0027185:15209-15228 Msa1432910:CDS 25.0%
! ATACTTCTAATTTGAGCTAA+GGG - tig0027185:15210-15229 Msa1432910:CDS 25.0%
! ATGATGAATGAGAAAATACT+TGG - tig0027185:15257-15276 Msa1432910:CDS 25.0%
! TGATGAATGAGAAAATACTT+GGG - tig0027185:15258-15277 Msa1432910:CDS 25.0%
!! TATATACTGATTGATTGATC+AGG - tig0027185:15301-15320 Msa1432910:CDS 25.0%
!!! TAAGAAAGTATTTTTGAGCA+TGG + tig0027185:15450-15469 None:intergenic 25.0%
! AAGACTTACTTTATATTCAG+TGG + tig0027185:15604-15623 None:intergenic 25.0%
! TGCTAATTCAGTAGTAAATT+TGG - tig0027185:15848-15867 Msa1432910:intron 25.0%
! GCTAATTCAGTAGTAAATTT+GGG - tig0027185:15849-15868 Msa1432910:intron 25.0%
! AAATTTGGGTCATAAATTTG+AGG - tig0027185:15863-15882 Msa1432910:CDS 25.0%
!!! ATTTTCTTGCTTTTCTGATT+TGG + tig0027185:15958-15977 None:intergenic 25.0%
! TTAATTAAGTGACGCTAATT+GGG + tig0027185:16115-16134 None:intergenic 25.0%
! ATTAATTAAGTGACGCTAAT+TGG + tig0027185:16116-16135 None:intergenic 25.0%
! GAACAAACGAATTAGTTTAG+AGG + tig0027185:14436-14455 None:intergenic 30.0%
! CTTTAATTGGTGGTTTGTAA+TGG - tig0027185:14502-14521 Msa1432910:three_prime_UTR 30.0%
! TTAATTGGTGGTTTGTAATG+GGG - tig0027185:14504-14523 Msa1432910:three_prime_UTR 30.0%
GTAAGATAGGACATAATTGT+AGG - tig0027185:14549-14568 Msa1432910:exon 30.0%
TATACCTTTCTTTCTCTCTT+GGG + tig0027185:14816-14835 None:intergenic 30.0%
ATATACCTTTCTTTCTCTCT+TGG + tig0027185:14817-14836 None:intergenic 30.0%
AATATGATATATTTGGCTGC+AGG - tig0027185:15788-15807 Msa1432910:intron 30.0%
! TTGTTTAAAGTAGGACAAAG+TGG + tig0027185:15925-15944 None:intergenic 30.0%
! TGCTTCTTCTTGTTTAAAGT+AGG + tig0027185:15934-15953 None:intergenic 30.0%
! TTGATTTTGTTCAAGCTTAG+GGG + tig0027185:16063-16082 None:intergenic 30.0%
!! GTTGATTTTGTTCAAGCTTA+GGG + tig0027185:16064-16083 None:intergenic 30.0%
ATCATATCTTCTTGTACATG+AGG + tig0027185:16218-16237 None:intergenic 30.0%
GTACAAGAAGATATGATCAA+TGG - tig0027185:16221-16240 Msa1432910:five_prime_UTR 30.0%
! GAACAAACGAATTAGTTTAG+AGG + tig0027185:14436-14455 None:intergenic 30.0%
! CTTTAATTGGTGGTTTGTAA+TGG - tig0027185:14502-14521 Msa1432910:three_prime_UTR 30.0%
! TTAATTGGTGGTTTGTAATG+GGG - tig0027185:14504-14523 Msa1432910:three_prime_UTR 30.0%
GTAAGATAGGACATAATTGT+AGG - tig0027185:14549-14568 Msa1432910:exon 30.0%
TATACCTTTCTTTCTCTCTT+GGG + tig0027185:14816-14835 None:intergenic 30.0%
ATATACCTTTCTTTCTCTCT+TGG + tig0027185:14817-14836 None:intergenic 30.0%
AATATGATATATTTGGCTGC+AGG - tig0027185:15788-15807 Msa1432910:intron 30.0%
! TTGTTTAAAGTAGGACAAAG+TGG + tig0027185:15925-15944 None:intergenic 30.0%
! TGCTTCTTCTTGTTTAAAGT+AGG + tig0027185:15934-15953 None:intergenic 30.0%
! TTGATTTTGTTCAAGCTTAG+GGG + tig0027185:16063-16082 None:intergenic 30.0%
!! GTTGATTTTGTTCAAGCTTA+GGG + tig0027185:16064-16083 None:intergenic 30.0%
ATCATATCTTCTTGTACATG+AGG + tig0027185:16218-16237 None:intergenic 30.0%
GTACAAGAAGATATGATCAA+TGG - tig0027185:16221-16240 Msa1432910:five_prime_UTR 30.0%
ACACACACACACACTTTAAT+TGG - tig0027185:14489-14508 Msa1432910:three_prime_UTR 35.0%
! GCTAAAGCTTTTCTTGATCA+TGG + tig0027185:14657-14676 None:intergenic 35.0%
! GATCAAGAAAAGCTTTAGCA+TGG - tig0027185:14658-14677 Msa1432910:CDS 35.0%
! TGATGTTGATGAAGAGAATG+AGG + tig0027185:14714-14733 None:intergenic 35.0%
ATACCTTTCTTTCTCTCTTG+GGG + tig0027185:14815-14834 None:intergenic 35.0%
TACATATGTTAGCGGAAAGA+TGG - tig0027185:14840-14859 Msa1432910:CDS 35.0%
AAACAAATGAAGGAAGCTGT+TGG + tig0027185:15043-15062 None:intergenic 35.0%
ATTTGAGCTAAGGGAGTTAA+TGG - tig0027185:15219-15238 Msa1432910:CDS 35.0%
AACACCAACACAAAAGAACA+AGG - tig0027185:15822-15841 Msa1432910:intron 35.0%
ACACCAACACAAAAGAACAA+GGG - tig0027185:15823-15842 Msa1432910:intron 35.0%
! ATACCCTTGTTCTTTTGTGT+TGG + tig0027185:15829-15848 None:intergenic 35.0%
TGTGAAGTTGAGATGAATGA+TGG - tig0027185:15897-15916 Msa1432910:CDS 35.0%
!! GGTTGATTTTGTTCAAGCTT+AGG + tig0027185:16065-16084 None:intergenic 35.0%
ATCTTCTTGTACATGAGGTT+AGG + tig0027185:16213-16232 None:intergenic 35.0%
ACACACACACACACTTTAAT+TGG - tig0027185:14489-14508 Msa1432910:three_prime_UTR 35.0%
! GCTAAAGCTTTTCTTGATCA+TGG + tig0027185:14657-14676 None:intergenic 35.0%
! GATCAAGAAAAGCTTTAGCA+TGG - tig0027185:14658-14677 Msa1432910:CDS 35.0%
! TGATGTTGATGAAGAGAATG+AGG + tig0027185:14714-14733 None:intergenic 35.0%
ATACCTTTCTTTCTCTCTTG+GGG + tig0027185:14815-14834 None:intergenic 35.0%
TACATATGTTAGCGGAAAGA+TGG - tig0027185:14840-14859 Msa1432910:CDS 35.0%
AAACAAATGAAGGAAGCTGT+TGG + tig0027185:15043-15062 None:intergenic 35.0%
ATTTGAGCTAAGGGAGTTAA+TGG - tig0027185:15219-15238 Msa1432910:CDS 35.0%
AACACCAACACAAAAGAACA+AGG - tig0027185:15822-15841 Msa1432910:intron 35.0%
ACACCAACACAAAAGAACAA+GGG - tig0027185:15823-15842 Msa1432910:intron 35.0%
! ATACCCTTGTTCTTTTGTGT+TGG + tig0027185:15829-15848 None:intergenic 35.0%
TGTGAAGTTGAGATGAATGA+TGG - tig0027185:15897-15916 Msa1432910:CDS 35.0%
!! GGTTGATTTTGTTCAAGCTT+AGG + tig0027185:16065-16084 None:intergenic 35.0%
ATCTTCTTGTACATGAGGTT+AGG + tig0027185:16213-16232 None:intergenic 35.0%
GTGTGTGTGAGAATGAGAAT+GGG + tig0027185:14475-14494 None:intergenic 40.0%
TGTGTGTGTGAGAATGAGAA+TGG + tig0027185:14476-14495 None:intergenic 40.0%
CACACACACACTTTAATTGG+TGG - tig0027185:14492-14511 Msa1432910:three_prime_UTR 40.0%
!! AGAAGAAAGTGTTCGCATTG+TGG - tig0027185:14527-14546 Msa1432910:three_prime_UTR 40.0%
TGTTCGCATTGTGGTAAGAT+AGG - tig0027185:14536-14555 Msa1432910:exon 40.0%
GATGAAGAGAATGAGGAAGA+TGG + tig0027185:14707-14726 None:intergenic 40.0%
TTGATTGATCAGGAGTTCCA+TGG - tig0027185:15311-15330 Msa1432910:CDS 40.0%
CTAGCTTCTCTAATCCAACA+AGG + tig0027185:15360-15379 None:intergenic 40.0%
! CTTGTTGGATTAGAGAAGCT+AGG - tig0027185:15358-15377 Msa1432910:intron 40.0%
GGATTAGAGAAGCTAGGAAA+AGG - tig0027185:15364-15383 Msa1432910:intron 40.0%
TATTTGTGGTTATGGGCTTG+TGG + tig0027185:15633-15652 None:intergenic 40.0%
TGATATATTTGGCTGCAGGT+TGG - tig0027185:15792-15811 Msa1432910:intron 40.0%
GATATATTTGGCTGCAGGTT+GGG - tig0027185:15793-15812 Msa1432910:intron 40.0%
! GAAAGTGTGAGGTCCAAATT+AGG + tig0027185:16032-16051 None:intergenic 40.0%
GTGTGTGTGAGAATGAGAAT+GGG + tig0027185:14475-14494 None:intergenic 40.0%
TGTGTGTGTGAGAATGAGAA+TGG + tig0027185:14476-14495 None:intergenic 40.0%
CACACACACACTTTAATTGG+TGG - tig0027185:14492-14511 Msa1432910:three_prime_UTR 40.0%
!! AGAAGAAAGTGTTCGCATTG+TGG - tig0027185:14527-14546 Msa1432910:three_prime_UTR 40.0%
TGTTCGCATTGTGGTAAGAT+AGG - tig0027185:14536-14555 Msa1432910:exon 40.0%
GATGAAGAGAATGAGGAAGA+TGG + tig0027185:14707-14726 None:intergenic 40.0%
TTGATTGATCAGGAGTTCCA+TGG - tig0027185:15311-15330 Msa1432910:CDS 40.0%
CTAGCTTCTCTAATCCAACA+AGG + tig0027185:15360-15379 None:intergenic 40.0%
! CTTGTTGGATTAGAGAAGCT+AGG - tig0027185:15358-15377 Msa1432910:intron 40.0%
GGATTAGAGAAGCTAGGAAA+AGG - tig0027185:15364-15383 Msa1432910:intron 40.0%
TATTTGTGGTTATGGGCTTG+TGG + tig0027185:15633-15652 None:intergenic 40.0%
TGATATATTTGGCTGCAGGT+TGG - tig0027185:15792-15811 Msa1432910:intron 40.0%
GATATATTTGGCTGCAGGTT+GGG - tig0027185:15793-15812 Msa1432910:intron 40.0%
! GAAAGTGTGAGGTCCAAATT+AGG + tig0027185:16032-16051 None:intergenic 40.0%
AAAGAGACGAAGTCCTCCAA+GGG + tig0027185:14594-14613 None:intergenic 45.0%
TTGGAGGACTTCGTCTCTTT+GGG - tig0027185:14594-14613 Msa1432910:CDS 45.0%
ACGAGGAGGATGATGATAGT+TGG + tig0027185:14622-14641 None:intergenic 45.0%
! CATGGTACTAGATGATGACG+AGG + tig0027185:14639-14658 None:intergenic 45.0%
AATGAGGAAGATGGAGAGGA+TGG + tig0027185:14698-14717 None:intergenic 45.0%
AGAGAATGAGGAAGATGGAG+AGG + tig0027185:14702-14721 None:intergenic 45.0%
GAGCACAGAACATTCCTTGT+TGG - tig0027185:15343-15362 Msa1432910:intron 45.0%
AGAAGCTAGGAAAAGGAGAC+TGG - tig0027185:15371-15390 Msa1432910:intron 45.0%
ACGTTCAAGTCTCTTCGACT+TGG - tig0027185:15495-15514 Msa1432910:intron 45.0%
TTGTGCAGCAGTGCCTAATT+TGG - tig0027185:16016-16035 Msa1432910:CDS 45.0%
AAAGAGACGAAGTCCTCCAA+GGG + tig0027185:14594-14613 None:intergenic 45.0%
TTGGAGGACTTCGTCTCTTT+GGG - tig0027185:14594-14613 Msa1432910:CDS 45.0%
ACGAGGAGGATGATGATAGT+TGG + tig0027185:14622-14641 None:intergenic 45.0%
! CATGGTACTAGATGATGACG+AGG + tig0027185:14639-14658 None:intergenic 45.0%
AATGAGGAAGATGGAGAGGA+TGG + tig0027185:14698-14717 None:intergenic 45.0%
AGAGAATGAGGAAGATGGAG+AGG + tig0027185:14702-14721 None:intergenic 45.0%
GAGCACAGAACATTCCTTGT+TGG - tig0027185:15343-15362 Msa1432910:intron 45.0%
AGAAGCTAGGAAAAGGAGAC+TGG - tig0027185:15371-15390 Msa1432910:intron 45.0%
ACGTTCAAGTCTCTTCGACT+TGG - tig0027185:15495-15514 Msa1432910:intron 45.0%
TTGTGCAGCAGTGCCTAATT+TGG - tig0027185:16016-16035 Msa1432910:CDS 45.0%
!!! AATTAAATAATTTTTATTTG+TGG + tig0027185:15647-15666 None:intergenic 5.0%
!! AATTAATAATATGATATATT+TGG - tig0027185:15781-15800 Msa1432910:intron 5.0%
!!! AATTAAATAATTTTTATTTG+TGG + tig0027185:15647-15666 None:intergenic 5.0%
!! AATTAATAATATGATATATT+TGG - tig0027185:15781-15800 Msa1432910:intron 5.0%
TGCACATCCTTCACTACCCT+TGG - tig0027185:14575-14594 Msa1432910:CDS 50.0%
ACATCCTTCACTACCCTTGG+AGG - tig0027185:14578-14597 Msa1432910:CDS 50.0%
AAGTCCTCCAAGGGTAGTGA+AGG + tig0027185:14585-14604 None:intergenic 50.0%
CAAAGAGACGAAGTCCTCCA+AGG + tig0027185:14595-14614 None:intergenic 50.0%
CTTGGAGGACTTCGTCTCTT+TGG - tig0027185:14593-14612 Msa1432910:CDS 50.0%
TGGAGGACTTCGTCTCTTTG+GGG - tig0027185:14595-14614 Msa1432910:CDS 50.0%
! GGTACTAGATGATGACGAGG+AGG + tig0027185:14636-14655 None:intergenic 50.0%
GAAGATGGAGAGGATGGTGA+GGG + tig0027185:14692-14711 None:intergenic 50.0%
CCTTTCTTTCTCTCTTGGGG+TGG + tig0027185:14812-14831 None:intergenic 50.0%
CCACCCCAAGAGAGAAAGAA+AGG - tig0027185:14809-14828 Msa1432910:CDS 50.0%
CTGTGCTCTTCTTCTGTCCA+TGG + tig0027185:15331-15350 None:intergenic 50.0%
CTAGGAAAAGGAGACTGGAG+AGG - tig0027185:15376-15395 Msa1432910:intron 50.0%
ATGGCTAGCGACTTGTGTAG+GGG + tig0027185:15431-15450 None:intergenic 50.0%
CATGGCTAGCGACTTGTGTA+GGG + tig0027185:15432-15451 None:intergenic 50.0%
! GGGATGCAACTGAAAGTGTG+AGG + tig0027185:16043-16062 None:intergenic 50.0%
AAGAAGGAATGAGCCAGCAG+GGG + tig0027185:16090-16109 None:intergenic 50.0%
CAAGAAGGAATGAGCCAGCA+GGG + tig0027185:16091-16110 None:intergenic 50.0%
TGACGCTAATTGGGCCAAGA+AGG + tig0027185:16106-16125 None:intergenic 50.0%
TGCACATCCTTCACTACCCT+TGG - tig0027185:14575-14594 Msa1432910:CDS 50.0%
ACATCCTTCACTACCCTTGG+AGG - tig0027185:14578-14597 Msa1432910:CDS 50.0%
AAGTCCTCCAAGGGTAGTGA+AGG + tig0027185:14585-14604 None:intergenic 50.0%
CAAAGAGACGAAGTCCTCCA+AGG + tig0027185:14595-14614 None:intergenic 50.0%
CTTGGAGGACTTCGTCTCTT+TGG - tig0027185:14593-14612 Msa1432910:CDS 50.0%
TGGAGGACTTCGTCTCTTTG+GGG - tig0027185:14595-14614 Msa1432910:CDS 50.0%
! GGTACTAGATGATGACGAGG+AGG + tig0027185:14636-14655 None:intergenic 50.0%
GAAGATGGAGAGGATGGTGA+GGG + tig0027185:14692-14711 None:intergenic 50.0%
CCTTTCTTTCTCTCTTGGGG+TGG + tig0027185:14812-14831 None:intergenic 50.0%
CCACCCCAAGAGAGAAAGAA+AGG - tig0027185:14809-14828 Msa1432910:CDS 50.0%
CTGTGCTCTTCTTCTGTCCA+TGG + tig0027185:15331-15350 None:intergenic 50.0%
CTAGGAAAAGGAGACTGGAG+AGG - tig0027185:15376-15395 Msa1432910:intron 50.0%
ATGGCTAGCGACTTGTGTAG+GGG + tig0027185:15431-15450 None:intergenic 50.0%
CATGGCTAGCGACTTGTGTA+GGG + tig0027185:15432-15451 None:intergenic 50.0%
! GGGATGCAACTGAAAGTGTG+AGG + tig0027185:16043-16062 None:intergenic 50.0%
AAGAAGGAATGAGCCAGCAG+GGG + tig0027185:16090-16109 None:intergenic 50.0%
CAAGAAGGAATGAGCCAGCA+GGG + tig0027185:16091-16110 None:intergenic 50.0%
TGACGCTAATTGGGCCAAGA+AGG + tig0027185:16106-16125 None:intergenic 50.0%
TGGAGAGGATGGTGAGGGAA+AGG + tig0027185:14687-14706 None:intergenic 55.0%
GGAAGATGGAGAGGATGGTG+AGG + tig0027185:14693-14712 None:intergenic 55.0%
GCATGGCTAGCGACTTGTGT+AGG + tig0027185:15433-15452 None:intergenic 55.0%
CAAAATCAACCCTCCCCTGC+TGG - tig0027185:16074-16093 Msa1432910:CDS 55.0%
CCAAGAAGGAATGAGCCAGC+AGG + tig0027185:16092-16111 None:intergenic 55.0%
!! CCTGCTGGCTCATTCCTTCT+TGG - tig0027185:16089-16108 Msa1432910:CDS 55.0%
TGGAGAGGATGGTGAGGGAA+AGG + tig0027185:14687-14706 None:intergenic 55.0%
GGAAGATGGAGAGGATGGTG+AGG + tig0027185:14693-14712 None:intergenic 55.0%
GCATGGCTAGCGACTTGTGT+AGG + tig0027185:15433-15452 None:intergenic 55.0%
CAAAATCAACCCTCCCCTGC+TGG - tig0027185:16074-16093 Msa1432910:CDS 55.0%
CCAAGAAGGAATGAGCCAGC+AGG + tig0027185:16092-16111 None:intergenic 55.0%
!! CCTGCTGGCTCATTCCTTCT+TGG - tig0027185:16089-16108 Msa1432910:CDS 55.0%
AGGAATGAGCCAGCAGGGGA+GGG + tig0027185:16086-16105 None:intergenic 60.0%
AAGGAATGAGCCAGCAGGGG+AGG + tig0027185:16087-16106 None:intergenic 60.0%
AGGAATGAGCCAGCAGGGGA+GGG + tig0027185:16086-16105 None:intergenic 60.0%
AAGGAATGAGCCAGCAGGGG+AGG + tig0027185:16087-16106 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0027185 gene 14429 16255 14429 ID=Msa1432910;Name=Msa1432910
tig0027185 mRNA 14429 16255 14429 ID=Msa1432910-mRNA-1;Parent=Msa1432910;Name=Msa1432910-mRNA-1;_AED=0.11;_eAED=0.11;_QI=92|1|1|1|1|1|3|125|274
tig0027185 exon 14429 14874 14429 ID=Msa1432910-mRNA-1:exon:8376;Parent=Msa1432910-mRNA-1
tig0027185 exon 15168 15361 15168 ID=Msa1432910-mRNA-1:exon:8375;Parent=Msa1432910-mRNA-1
tig0027185 exon 15854 16255 15854 ID=Msa1432910-mRNA-1:exon:8374;Parent=Msa1432910-mRNA-1
tig0027185 five_prime_UTR 16164 16255 16164 ID=Msa1432910-mRNA-1:five_prime_utr;Parent=Msa1432910-mRNA-1
tig0027185 CDS 15854 16163 15854 ID=Msa1432910-mRNA-1:cds;Parent=Msa1432910-mRNA-1
tig0027185 CDS 15168 15361 15168 ID=Msa1432910-mRNA-1:cds;Parent=Msa1432910-mRNA-1
tig0027185 CDS 14554 14874 14554 ID=Msa1432910-mRNA-1:cds;Parent=Msa1432910-mRNA-1
tig0027185 three_prime_UTR 14429 14553 14429 ID=Msa1432910-mRNA-1:three_prime_utr;Parent=Msa1432910-mRNA-1
Gene Sequence

>Msa1432910

ATGGGGAGAAGAAAGTGTTCGCATTGTGGTAAGATAGGACATAATTGTAGGACATGCACATCCTTCACTACCCTTGGAGGACTTCGTCTCTTTGGGGTCCAACTATCATCATCCTCCTCGTCATCATCTAGTACCATGATCAAGAAAAGCTTTAGCATGGACACCTTTCCCTCACCATCCTCTCCATCTTCCTCATTCTCTTCATCAACATCATTAACCAATATTGATGAAAATTATTATCACAAACCCACTTCTAATATTTCATATCTATCAGATTGTTTTATAGGTCCACCCCAAGAGAGAAAGAAAGGAGTTCCATGGACAGAAGAAGAGCACAGAACATTCCTTGTTGGATTAGAGAAGCTAGGAAAAGGAGACTGGAGAGGCATCTCTAAAAACTTTGTCACTTCAAGAACCCCTACACAAGTCGCTAGCCATGCTCAAAAATACTTTCTTAGATTGGCTACTATCAACAAGAAGCGACGTTCAAGTCTCTTCGACTTGGTTGGGAGCAAGAACACCAACACAAAAGAACAAGGGTATGCTAATTCAGTAGTAAATTTGGGTCATAAATTTGAGGATAAGTGTAAATGTGAAGTTGAGATGAATGATGGCACCACTTTGTCCTACTTTAAACAAGAAGAAGCAGCCAAATCAGAAAAGCAAGAAAATAATTACTCTACAGATAATTGGCTACATGACTCTTCAAATTGTGCAGCAGTGCCTAATTTGGACCTCACACTTTCAGTTGCATCCCCTAAGCTTGAACAAAATCAACCCTCCCCTGCTGGCTCATTCCTTCTTGGCCCAATTAGCGTCACTTAA
Protein sequence

>Msa1432910

MGRRKCSHCGKIGHNCRTCTSFTTLGGLRLFGVQLSSSSSSSSSTMIKKSFSMDTFPSPSSPSSSFSSSTSLTNIDENYYHKPTSNISYLSDCFIGPPQERKKGVPWTEEEHRTFLVGLEKLGKGDWRGISKNFVTSRTPTQVASHAQKYFLRLATINKKRRSSLFDLVGSKNTNTKEQGYANSVVNLGHKFEDKCKCEVEMNDGTTLSYFKQEEAAKSEKQENNYSTDNWLHDSSNCAAVPNLDLTLSVASPKLEQNQPSPAGSFLLGPISVT*