AlfalfaGEDB | Msa1433000

M. sativa cultivar zhongmu-4 / Msa1433000

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433000	AES61528.1	100.000	76	0	0	1	76	25	100	3.02e-48	157

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433000	G7IAE7	100.000	76	0	0	1	76	25	100	1.44e-48	157

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1356960	Msa1433000	0.857612	1.449816e-62	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1433000	MtrunA17_Chr1g0193451	100.000	76	0	0	1	76	25	100	2.78e-52	157

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 12 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTGCTTTAGATTAACA+TGG	0.562152	tig0027216:-53310	None:intergenic
AGATTCTCTTCACACAATTC+AGG	0.309458	tig0027216:-53209	None:intergenic
AGATTGCTTTAGATTAACAT+GGG	0.597388	tig0027216:-53309	None:intergenic
AGGACATGGAAGAAATTCTA+AGG	0.339186	tig0027216:+53159	None:intergenic
ATCCTCATCATGTGTCTTGG+TGG	0.548849	tig0027216:-53239	None:intergenic
ATGAGGATTTCAAAGAAGCA+AGG	0.570103	tig0027216:+53254	Msa1433000:CDS
CACCACCAAGACACATGATG+AGG	0.657704	tig0027216:+53237	Msa1433000:CDS
GAAATCCTCATCATGTGTCT+TGG	0.471233	tig0027216:-53242	None:intergenic
GATTGCTTTAGATTAACATG+GGG	0.689330	tig0027216:-53308	None:intergenic
GGACATGGAAGAAATTCTAA+GGG	0.641098	tig0027216:+53160	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTCAAAAAGTAACTTTGCT+GGG	+	tig0027216:53357-53376	Msa1433000:CDS	30.0%
AGATTCTCTTCACACAATTC+AGG	-	tig0027216:53212-53231	None:intergenic	35.0%
ATGAGGATTTCAAAGAAGCA+AGG	+	tig0027216:53254-53273	Msa1433000:CDS	35.0%
TGAGGATTTCAAAGAAGCAA+GGG	+	tig0027216:53255-53274	Msa1433000:CDS	35.0%
CAAGGGAAAAAGAATAAAGC+AGG	+	tig0027216:53272-53291	Msa1433000:CDS	35.0%
GGAAAAAGAATAAAGCAGGA+CGG	+	tig0027216:53276-53295	Msa1433000:CDS	35.0%
GCTTCAAAAAGTAACTTTGC+TGG	+	tig0027216:53356-53375	Msa1433000:CDS	35.0%
GAAATCCTCATCATGTGTCT+TGG	-	tig0027216:53245-53264	None:intergenic	40.0%
ATCCTCATCATGTGTCTTGG+TGG	-	tig0027216:53242-53261	None:intergenic	45.0%
CACCACCAAGACACATGATG+AGG	+	tig0027216:53237-53256	Msa1433000:CDS	50.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0027216	gene	53164	53394	53164	ID=Msa1433000;Name=Msa1433000
tig0027216	mRNA	53164	53394	53164	ID=Msa1433000-mRNA-1;Parent=Msa1433000;Name=Msa1433000-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|76
tig0027216	exon	53164	53394	53164	ID=Msa1433000-mRNA-1:exon:8379;Parent=Msa1433000-mRNA-1
tig0027216	CDS	53164	53394	53164	ID=Msa1433000-mRNA-1:cds;Parent=Msa1433000-mRNA-1

Gene Sequence	>Msa1433000 ATGGAAGAAATTCTAAGGGCAAAGCTTTCTACTATCATTGAAGAACCTGAATTGTGTGAAGAGAATCTAACATCACCACCAAGACACATGATGAGGATTTCAAAGAAGCAAGGGAAAAAGAATAAAGCAGGACGGTGTTTAGTACCCCATGTTAATCTAAAGCAATCTTATATTCTCTTTATTAATGGCTTTGCTTCAAAAAGTAACTTTGCTGGGTTTCTTCAACGCTAA
Protein sequence	>Msa1433000 MEEILRAKLSTIIEEPELCEENLTSPPRHMMRISKKQGKKNKAGRCLVPHVNLKQSYILFINGFASKSNFAGFLQR*