Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433000 | AES61528.1 | 100.000 | 76 | 0 | 0 | 1 | 76 | 25 | 100 | 3.02e-48 | 157 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433000 | G7IAE7 | 100.000 | 76 | 0 | 0 | 1 | 76 | 25 | 100 | 1.44e-48 | 157 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1356960 | Msa1433000 | 0.857612 | 1.449816e-62 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1433000 | MtrunA17_Chr1g0193451 | 100.000 | 76 | 0 | 0 | 1 | 76 | 25 | 100 | 2.78e-52 | 157 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTATATTCTCTTTATTAA+TGG | 0.166144 | tig0027216:+53329 | Msa1433000:CDS |
| AGATTCTCTTCACACAATTC+AGG | 0.309458 | tig0027216:-53209 | None:intergenic |
| AGGACATGGAAGAAATTCTA+AGG | 0.339186 | tig0027216:+53159 | None:intergenic |
| GAAATCCTCATCATGTGTCT+TGG | 0.471233 | tig0027216:-53242 | None:intergenic |
| ATCCTCATCATGTGTCTTGG+TGG | 0.548849 | tig0027216:-53239 | None:intergenic |
| AAGATTGCTTTAGATTAACA+TGG | 0.562152 | tig0027216:-53310 | None:intergenic |
| ATGAGGATTTCAAAGAAGCA+AGG | 0.570103 | tig0027216:+53254 | Msa1433000:CDS |
| AGATTGCTTTAGATTAACAT+GGG | 0.597388 | tig0027216:-53309 | None:intergenic |
| TGAGGATTTCAAAGAAGCAA+GGG | 0.598782 | tig0027216:+53255 | Msa1433000:CDS |
| GGACATGGAAGAAATTCTAA+GGG | 0.641098 | tig0027216:+53160 | None:intergenic |
| CACCACCAAGACACATGATG+AGG | 0.657704 | tig0027216:+53237 | Msa1433000:CDS |
| GATTGCTTTAGATTAACATG+GGG | 0.689330 | tig0027216:-53308 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTATATTCTCTTTATTAA+TGG | + | tig0027216:53329-53348 | Msa1433000:CDS | 15.0% |
| !! | AGATTGCTTTAGATTAACAT+GGG | - | tig0027216:53312-53331 | None:intergenic | 25.0% |
| !! | AAGATTGCTTTAGATTAACA+TGG | - | tig0027216:53313-53332 | None:intergenic | 25.0% |
| ! | GATTGCTTTAGATTAACATG+GGG | - | tig0027216:53311-53330 | None:intergenic | 30.0% |
| CTTCAAAAAGTAACTTTGCT+GGG | + | tig0027216:53357-53376 | Msa1433000:CDS | 30.0% | |
| AGATTCTCTTCACACAATTC+AGG | - | tig0027216:53212-53231 | None:intergenic | 35.0% | |
| ATGAGGATTTCAAAGAAGCA+AGG | + | tig0027216:53254-53273 | Msa1433000:CDS | 35.0% | |
| TGAGGATTTCAAAGAAGCAA+GGG | + | tig0027216:53255-53274 | Msa1433000:CDS | 35.0% | |
| CAAGGGAAAAAGAATAAAGC+AGG | + | tig0027216:53272-53291 | Msa1433000:CDS | 35.0% | |
| GGAAAAAGAATAAAGCAGGA+CGG | + | tig0027216:53276-53295 | Msa1433000:CDS | 35.0% | |
| GCTTCAAAAAGTAACTTTGC+TGG | + | tig0027216:53356-53375 | Msa1433000:CDS | 35.0% | |
| GAAATCCTCATCATGTGTCT+TGG | - | tig0027216:53245-53264 | None:intergenic | 40.0% | |
| ATCCTCATCATGTGTCTTGG+TGG | - | tig0027216:53242-53261 | None:intergenic | 45.0% | |
| CACCACCAAGACACATGATG+AGG | + | tig0027216:53237-53256 | Msa1433000:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027216 | gene | 53164 | 53394 | 53164 | ID=Msa1433000;Name=Msa1433000 |
| tig0027216 | mRNA | 53164 | 53394 | 53164 | ID=Msa1433000-mRNA-1;Parent=Msa1433000;Name=Msa1433000-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|76 |
| tig0027216 | exon | 53164 | 53394 | 53164 | ID=Msa1433000-mRNA-1:exon:8379;Parent=Msa1433000-mRNA-1 |
| tig0027216 | CDS | 53164 | 53394 | 53164 | ID=Msa1433000-mRNA-1:cds;Parent=Msa1433000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
