Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1356960 | AES61528.1 | 99.000 | 100 | 1 | 0 | 1 | 100 | 1 | 100 | 9.31e-66 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1356960 | G7IAE7 | 99.000 | 100 | 1 | 0 | 1 | 100 | 1 | 100 | 4.44e-66 | 203 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1356960 | Msa1433000 | 0.857612 | 1.449816e-62 | -8.615850e-47 |
| Msa0217670 | Msa1356960 | 0.807418 | 5.103493e-50 | -8.615850e-47 |
| Msa0237300 | Msa1356960 | 0.803055 | 4.183704e-49 | -8.615850e-47 |
| Msa0439960 | Msa1356960 | 0.804859 | 1.764888e-49 | -8.615850e-47 |
| Msa0992510 | Msa1356960 | 0.804884 | 1.743518e-49 | -8.615850e-47 |
| Msa0274720 | Msa1356960 | 0.817750 | 2.805120e-52 | -8.615850e-47 |
| Msa0315520 | Msa1356960 | 0.807232 | 5.590467e-50 | -8.615850e-47 |
| Msa0910960 | Msa1356960 | 0.819386 | 1.193249e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1356960 | MtrunA17_Chr1g0193451 | 99.000 | 100 | 1 | 0 | 1 | 100 | 1 | 100 | 8.56e-70 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTATATTCTCTTTATTAA+TGG | 0.166144 | tig0015878:+21808 | Msa1356960:CDS |
| AGATTCTCTTCACACAATTC+AGG | 0.309458 | tig0015878:-21688 | None:intergenic |
| AGGACATGGAAGAAATTCTA+AGG | 0.339186 | tig0015878:+21638 | Msa1356960:CDS |
| GGGCATGCAGGAAAATATTA+AGG | 0.375591 | tig0015878:+21578 | Msa1356960:CDS |
| GAAATCCTCATCATGTGTCT+TGG | 0.471233 | tig0015878:-21721 | None:intergenic |
| AAGAAATGAGGAGGGCATGC+AGG | 0.501666 | tig0015878:+21566 | None:intergenic |
| CACTTCTCCTGCTCGTGCAA+GGG | 0.516284 | tig0015878:-21606 | None:intergenic |
| TCACTTCTCCTGCTCGTGCA+AGG | 0.517138 | tig0015878:-21607 | None:intergenic |
| GGTCAGTCCCTTGCACGAGC+AGG | 0.526768 | tig0015878:+21599 | Msa1356960:CDS |
| ATCCTCATCATGTGTCTTGG+TGG | 0.548849 | tig0015878:-21718 | None:intergenic |
| CGAGCAGGAGAAGTGAGTCT+AGG | 0.560331 | tig0015878:+21614 | Msa1356960:CDS |
| AAGATTGCTTTAGATTAACA+TGG | 0.562152 | tig0015878:-21789 | None:intergenic |
| ATGAGGATTTCAAAGAAGCA+AGG | 0.570103 | tig0015878:+21733 | Msa1356960:CDS |
| CAGGAGAAGTGAGTCTAGGA+AGG | 0.590561 | tig0015878:+21618 | Msa1356960:CDS |
| AGATTGCTTTAGATTAACAT+GGG | 0.597388 | tig0015878:-21788 | None:intergenic |
| TGAGGATTTCAAAGAAGCAA+GGG | 0.598782 | tig0015878:+21734 | Msa1356960:CDS |
| AAGTGAGTCTAGGAAGGACA+TGG | 0.600576 | tig0015878:+21624 | Msa1356960:CDS |
| CAGAAGCAAAAGAAATGAGG+AGG | 0.606480 | tig0015878:+21557 | None:intergenic |
| GGACATGGAAGAAATTCTAA+GGG | 0.641098 | tig0015878:+21639 | Msa1356960:CDS |
| CACCACCAAGACACATGATG+AGG | 0.657704 | tig0015878:+21716 | Msa1356960:CDS |
| AGAAGCAAAAGAAATGAGGA+GGG | 0.660259 | tig0015878:+21558 | None:intergenic |
| GATTGCTTTAGATTAACATG+GGG | 0.689330 | tig0015878:-21787 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTATATTCTCTTTATTAA+TGG | + | tig0015878:21808-21827 | Msa1356960:CDS | 15.0% |
| !! | AGATTGCTTTAGATTAACAT+GGG | - | tig0015878:21791-21810 | None:intergenic | 25.0% |
| !! | AAGATTGCTTTAGATTAACA+TGG | - | tig0015878:21792-21811 | None:intergenic | 25.0% |
| ! | GATTGCTTTAGATTAACATG+GGG | - | tig0015878:21790-21809 | None:intergenic | 30.0% |
| CTTCAAAAAGTAACTTTGCT+GGG | + | tig0015878:21836-21855 | Msa1356960:CDS | 30.0% | |
| AGGACATGGAAGAAATTCTA+AGG | + | tig0015878:21638-21657 | Msa1356960:CDS | 35.0% | |
| GGACATGGAAGAAATTCTAA+GGG | + | tig0015878:21639-21658 | Msa1356960:CDS | 35.0% | |
| AGATTCTCTTCACACAATTC+AGG | - | tig0015878:21691-21710 | None:intergenic | 35.0% | |
| ATGAGGATTTCAAAGAAGCA+AGG | + | tig0015878:21733-21752 | Msa1356960:CDS | 35.0% | |
| TGAGGATTTCAAAGAAGCAA+GGG | + | tig0015878:21734-21753 | Msa1356960:CDS | 35.0% | |
| CAAGGGAAAAAGAATAAAGC+AGG | + | tig0015878:21751-21770 | Msa1356960:CDS | 35.0% | |
| GGAAAAAGAATAAAGCAGGA+CGG | + | tig0015878:21755-21774 | Msa1356960:CDS | 35.0% | |
| GCTTCAAAAAGTAACTTTGC+TGG | + | tig0015878:21835-21854 | Msa1356960:CDS | 35.0% | |
| GGGCATGCAGGAAAATATTA+AGG | + | tig0015878:21578-21597 | Msa1356960:CDS | 40.0% | |
| GAAATCCTCATCATGTGTCT+TGG | - | tig0015878:21724-21743 | None:intergenic | 40.0% | |
| AAGTGAGTCTAGGAAGGACA+TGG | + | tig0015878:21624-21643 | Msa1356960:CDS | 45.0% | |
| ATCCTCATCATGTGTCTTGG+TGG | - | tig0015878:21721-21740 | None:intergenic | 45.0% | |
| CAGGAGAAGTGAGTCTAGGA+AGG | + | tig0015878:21618-21637 | Msa1356960:CDS | 50.0% | |
| CACCACCAAGACACATGATG+AGG | + | tig0015878:21716-21735 | Msa1356960:CDS | 50.0% | |
| CACTTCTCCTGCTCGTGCAA+GGG | - | tig0015878:21609-21628 | None:intergenic | 55.0% | |
| TCACTTCTCCTGCTCGTGCA+AGG | - | tig0015878:21610-21629 | None:intergenic | 55.0% | |
| CGAGCAGGAGAAGTGAGTCT+AGG | + | tig0015878:21614-21633 | Msa1356960:CDS | 55.0% | |
| GGTCAGTCCCTTGCACGAGC+AGG | + | tig0015878:21599-21618 | Msa1356960:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0015878 | gene | 21571 | 21873 | 21571 | ID=Msa1356960;Name=Msa1356960 |
| tig0015878 | mRNA | 21571 | 21873 | 21571 | ID=Msa1356960-mRNA-1;Parent=Msa1356960;Name=Msa1356960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|100 |
| tig0015878 | exon | 21571 | 21873 | 21571 | ID=Msa1356960-mRNA-1:exon:10215;Parent=Msa1356960-mRNA-1 |
| tig0015878 | CDS | 21571 | 21873 | 21571 | ID=Msa1356960-mRNA-1:cds;Parent=Msa1356960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
