AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1433080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433080 MtrunA17_Chr2g0320621 97.071 478 13 1 1 478 1 477 0.0 934
Msa1433080 MtrunA17_Chr4g0027681 75.267 469 111 2 6 470 1 468 0.0 733
Msa1433080 MtrunA17_Chr7g0265331 63.011 465 169 3 4 466 5 468 0.0 612
Msa1433080 MtrunA17_Chr1g0179751 60.917 458 171 5 5 458 34 487 0.0 570
Msa1433080 MtrunA17_Chr1g0189381 41.228 456 245 8 18 467 19 457 4.89e-111 337
Msa1433080 MtrunA17_Chr7g0249451 39.462 446 249 9 8 445 5 437 1.46e-104 320
Msa1433080 MtrunA17_Chr7g0221681 37.634 465 265 9 19 473 18 467 3.51e-100 309
Msa1433080 MtrunA17_Chr5g0401601 32.426 404 230 12 53 439 62 439 1.91e-53 190
Msa1433080 MtrunA17_Chr4g0019931 72.131 122 34 0 83 204 1 122 5.84e-52 171
Msa1433080 MtrunA17_Chr4g0053721 31.842 380 215 11 77 439 79 431 5.41e-50 180
Msa1433080 MtrunA17_Chr1g0159791 30.435 391 228 12 65 438 46 409 6.77e-45 162
Msa1433080 MtrunA17_Chr3g0130931 31.915 376 214 17 77 439 103 449 9.30e-42 154
Msa1433080 MtrunA17_Chr5g0430861 88.750 80 6 1 204 280 27 106 4.48e-39 137
Msa1433080 MtrunA17_Chr4g0010951 29.126 412 218 18 77 439 60 446 3.38e-38 144
Msa1433080 MtrunA17_Chr6g0484731 25.926 405 255 11 65 448 180 560 1.71e-36 142
Msa1433080 MtrunA17_Chr8g0371351 29.126 412 252 14 42 438 63 449 3.19e-36 140
Msa1433080 MtrunA17_Chr2g0310801 32.353 374 214 20 77 439 89 434 7.90e-36 137
Msa1433080 MtrunA17_Chr8g0371371 27.427 412 258 14 46 442 78 463 2.37e-35 138
Msa1433080 MtrunA17_Chr5g0442921 28.871 381 237 12 77 439 190 554 5.87e-35 137
Msa1433080 MtrunA17_Chr3g0130741 27.833 406 240 18 53 439 64 435 7.82e-35 135
Msa1433080 MtrunA17_Chr8g0384731 27.089 395 237 16 65 439 62 425 9.33e-35 135
Msa1433080 MtrunA17_Chr2g0310821 30.239 377 213 18 77 441 107 445 1.68e-34 134
Msa1433080 MtrunA17_Chr3g0095891 29.659 381 237 13 74 438 93 458 5.16e-34 133
Msa1433080 MtrunA17_Chr5g0406001 27.765 443 264 19 43 457 72 486 7.48e-33 130
Msa1433080 MtrunA17_Chr5g0435871 30.933 375 220 15 77 439 88 435 2.19e-31 125
Msa1433080 MtrunA17_Chr8g0386441 30.769 377 216 18 77 439 85 430 2.51e-31 125
Msa1433080 MtrunA17_Chr2g0307991 29.211 380 216 16 79 439 60 405 6.79e-31 123
Msa1433080 MtrunA17_Chr2g0310831 28.497 386 216 18 77 439 93 441 1.02e-30 123
Msa1433080 MtrunA17_Chr8g0352671 28.760 379 227 16 76 439 87 437 3.21e-30 122
Msa1433080 MtrunA17_Chr5g0435971 30.667 375 221 15 77 439 85 432 7.65e-30 120
Msa1433080 MtrunA17_Chr2g0308001 28.647 377 227 13 79 439 59 409 1.25e-29 120
Msa1433080 MtrunA17_Chr3g0087851 29.134 381 235 16 70 439 81 437 3.48e-29 119
Msa1433080 MtrunA17_Chr8g0352661 30.184 381 220 17 77 439 85 437 4.93e-29 119
Msa1433080 MtrunA17_Chr7g0267171 28.856 402 221 19 77 438 89 465 6.02e-29 119
Msa1433080 MtrunA17_Chr2g0291201 28.496 379 226 16 77 438 101 451 2.82e-28 116
Msa1433080 MtrunA17_Chr1g0165201 27.920 351 210 16 77 415 127 446 3.50e-28 116
Msa1433080 MtrunA17_Chr1g0212321 29.679 374 219 16 77 438 145 486 1.35e-27 115
Msa1433080 MtrunA17_Chr5g0419291 30.108 372 222 15 77 438 85 428 2.01e-27 114
Msa1433080 MtrunA17_Chr5g0395911 26.241 423 258 18 36 439 27 414 2.33e-27 113
Msa1433080 MtrunA17_Chr1g0210201 26.558 369 233 11 77 438 130 467 2.82e-27 114
Msa1433080 MtrunA17_Chr5g0415951 27.851 377 238 13 73 439 83 435 2.89e-27 113
Msa1433080 MtrunA17_Chr3g0124421 27.838 370 240 13 78 438 99 450 7.20e-27 113
Msa1433080 MtrunA17_Chr2g0309561 24.706 425 249 12 64 438 105 508 1.32e-26 112
Msa1433080 MtrunA17_Chr6g0484901 27.596 366 228 12 79 438 157 491 2.29e-26 111
Msa1433080 MtrunA17_Chr7g0275731 27.851 377 225 14 77 438 140 484 3.22e-26 111
Msa1433080 MtrunA17_Chr5g0395931 28.087 413 238 21 48 439 62 436 6.01e-26 109
Msa1433080 MtrunA17_Chr2g0333991 26.110 383 230 16 75 437 85 434 9.35e-25 106
Msa1433080 MtrunA17_Chr3g0099891 27.346 373 228 11 77 439 116 455 1.76e-24 105
Msa1433080 MtrunA17_Chr5g0395871 27.895 380 223 17 77 439 80 425 3.06e-24 104
Msa1433080 MtrunA17_Chr3g0078321 24.235 392 235 15 76 439 56 413 9.46e-24 103
Msa1433080 MtrunA17_Chr8g0353261 27.467 375 229 15 75 439 93 434 9.93e-24 103
Msa1433080 MtrunA17_Chr6g0457401 28.871 381 234 16 68 439 85 437 1.07e-23 103
Msa1433080 MtrunA17_Chr6g0486981 27.297 381 225 16 79 439 89 437 1.67e-23 102
Msa1433080 MtrunA17_Chr2g0311451 25.571 438 259 19 23 439 45 436 2.83e-23 102
Msa1433080 MtrunA17_Chr5g0425141 25.197 381 255 13 76 447 99 458 3.40e-23 102
Msa1433080 MtrunA17_Chr8g0390761 25.895 363 228 12 79 431 117 448 5.72e-23 101
Msa1433080 MtrunA17_Chr3g0099901 27.688 372 232 11 77 438 141 485 6.27e-23 101
Msa1433080 MtrunA17_Chr4g0023351 30.894 246 144 9 79 314 59 288 1.05e-22 98.2
Msa1433080 MtrunA17_Chr1g0164401 26.202 416 246 21 42 439 57 429 1.99e-22 99.4
Msa1433080 MtrunA17_Chr6g0487471 26.842 380 232 15 77 439 85 435 2.46e-22 99.4
Msa1433080 MtrunA17_Chr4g0001991 30.214 374 194 18 94 440 186 519 3.50e-22 99.4
Msa1433080 MtrunA17_Chr3g0110191 25.945 397 214 20 83 443 91 443 7.80e-22 97.8
Msa1433080 MtrunA17_Chr2g0312941 24.344 419 263 17 50 433 49 448 7.99e-21 94.7
Msa1433080 MtrunA17_Chr2g0307461 25.123 406 258 14 49 429 37 421 2.53e-20 93.2
Msa1433080 MtrunA17_Chr7g0239001 27.506 389 226 17 83 440 64 427 6.67e-20 92.0
Msa1433080 MtrunA17_Chr4g0056211 27.981 411 210 23 89 439 87 471 7.62e-19 89.0
Msa1433080 MtrunA17_Chr5g0434441 25.831 391 244 14 83 451 73 439 3.85e-17 83.6
Msa1433080 MtrunA17_Chr5g0434411 23.760 383 236 17 83 439 86 438 3.94e-17 83.6
Msa1433080 MtrunA17_Chr4g0061641 28.800 375 220 19 79 439 56 397 7.97e-17 82.4
Msa1433080 MtrunA17_Chr3g0112051 24.543 383 215 19 83 440 78 411 2.60e-15 77.8
Msa1433080 MtrunA17_Chr1g0179091 29.084 251 144 12 202 439 138 367 1.54e-14 75.1
Msa1433080 MtrunA17_Chr3g0133071 27.368 380 228 17 74 439 93 438 9.43e-14 73.2
Msa1433080 MtrunA17_Chr2g0287841 20.655 397 242 15 72 425 62 428 1.88e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433080 AT5G22850.1 72.786 463 122 2 1 460 1 462 0.0 689
Msa1433080 AT1G08210.3 62.151 465 173 3 2 466 55 516 0.0 564
Msa1433080 AT1G08210.4 62.151 465 173 3 2 466 41 502 0.0 563
Msa1433080 AT1G08210.1 62.151 465 173 3 2 466 7 468 0.0 563
Msa1433080 AT2G36670.2 60.000 455 172 4 11 460 27 476 0.0 561
Msa1433080 AT2G36670.1 59.348 460 172 5 11 460 27 481 0.0 555
Msa1433080 AT1G08210.2 58.740 492 173 4 2 466 7 495 0.0 548
Msa1433080 AT2G36670.4 62.663 383 138 3 78 460 33 410 5.79e-176 501
Msa1433080 AT2G36670.3 62.663 383 138 3 78 460 33 410 5.79e-176 501
Msa1433080 AT3G02740.1 39.062 448 245 9 5 439 8 440 7.65e-106 324
Msa1433080 AT1G05840.1 39.024 451 255 8 7 451 14 450 2.90e-105 322
Msa1433080 AT5G36260.1 40.528 417 227 8 39 449 43 444 5.99e-103 316
Msa1433080 AT3G02740.2 41.979 374 202 7 71 439 2 365 5.91e-98 301
Msa1433080 AT1G65240.1 40.144 416 225 11 40 449 40 437 1.54e-94 294
Msa1433080 AT3G42550.1 38.117 446 203 11 10 442 8 393 1.97e-88 277
Msa1433080 AT5G43100.2 33.065 372 213 12 77 439 74 418 4.97e-58 199
Msa1433080 AT5G43100.1 33.065 372 213 12 77 439 74 418 9.63e-57 199
Msa1433080 AT3G50050.1 31.078 399 239 11 51 439 65 437 9.24e-46 169
Msa1433080 AT3G25700.1 31.070 383 232 13 77 440 82 451 2.66e-44 162
Msa1433080 AT1G44130.1 28.465 404 241 14 54 439 27 400 2.48e-42 155
Msa1433080 AT3G51350.1 29.187 418 246 13 43 438 63 452 2.55e-40 152
Msa1433080 AT2G17760.1 29.126 412 248 18 43 438 66 449 1.42e-36 141
Msa1433080 AT1G77480.1 28.074 431 265 13 63 475 53 456 5.58e-36 139
Msa1433080 AT3G51340.2 29.040 427 266 13 25 438 45 447 7.07e-36 139
Msa1433080 AT2G03200.1 28.721 383 223 15 77 438 105 458 1.01e-35 138
Msa1433080 AT1G64830.1 30.914 372 222 12 77 439 84 429 2.40e-35 137
Msa1433080 AT3G59080.1 28.796 382 237 12 77 439 168 533 3.56e-35 138
Msa1433080 AT4G33490.2 27.014 422 242 19 53 447 38 420 5.02e-35 135
Msa1433080 AT1G49050.1 26.279 430 257 17 40 438 168 568 5.88e-35 138
Msa1433080 AT3G51330.3 29.767 430 268 15 25 439 44 454 1.17e-34 135
Msa1433080 AT5G10080.1 28.989 376 231 11 78 438 99 453 1.37e-34 136
Msa1433080 AT3G51330.1 29.837 429 267 15 25 438 44 453 2.15e-34 135
Msa1433080 AT1G49050.2 25.840 387 241 14 78 438 29 395 2.34e-34 133
Msa1433080 AT4G35880.1 29.380 371 227 15 78 438 106 451 2.52e-34 135
Msa1433080 AT1G77480.2 27.919 394 239 13 63 438 53 419 4.83e-34 133
Msa1433080 AT2G35615.1 28.025 471 280 18 1 439 1 444 3.33e-32 128
Msa1433080 AT3G51360.1 28.341 434 269 18 36 452 41 449 4.72e-32 128
Msa1433080 AT1G09750.1 30.159 378 223 14 74 438 99 448 4.91e-32 127
Msa1433080 AT3G51360.9 28.341 434 269 18 36 452 51 459 6.64e-32 128
Msa1433080 AT5G10770.1 29.491 373 225 13 77 439 130 474 7.93e-32 127
Msa1433080 AT3G51360.7 28.278 389 244 14 75 452 1 365 1.15e-31 126
Msa1433080 AT2G42980.1 27.604 384 241 13 77 439 158 525 3.76e-31 126
Msa1433080 AT3G51330.2 29.736 417 259 15 25 426 44 441 8.41e-31 124
Msa1433080 AT4G35880.2 29.970 337 203 14 78 404 106 419 1.62e-30 123
Msa1433080 AT2G23945.1 28.191 376 239 11 75 438 92 448 1.89e-30 123
Msa1433080 AT3G51340.3 30.081 369 225 11 25 380 45 393 2.14e-30 122
Msa1433080 AT1G25510.1 28.726 369 225 14 77 438 146 483 3.96e-30 122
Msa1433080 AT3G61820.1 29.178 377 226 16 77 438 133 483 1.25e-29 121
Msa1433080 AT4G30030.1 27.151 372 233 12 79 440 78 421 1.96e-29 120
Msa1433080 AT1G01300.1 30.239 377 218 15 77 439 140 485 7.82e-29 119
Msa1433080 AT3G51340.1 27.189 434 277 12 25 438 45 459 2.11e-28 118
Msa1433080 AT5G33340.1 29.600 375 225 16 77 439 88 435 2.80e-28 117
Msa1433080 AT3G51340.4 30.330 333 204 9 25 346 45 360 7.11e-28 115
Msa1433080 AT3G12700.1 25.600 375 246 14 79 439 106 461 1.01e-27 115
Msa1433080 AT3G51360.10 29.367 395 237 18 36 413 41 410 1.33e-27 115
Msa1433080 AT3G51360.6 29.367 395 237 18 36 413 51 420 1.54e-27 115
Msa1433080 AT4G30040.1 29.375 320 183 12 79 379 85 380 1.61e-27 114
Msa1433080 AT3G54400.1 28.075 374 228 15 75 439 84 425 1.87e-27 114
Msa1433080 AT4G33490.1 27.095 358 202 16 53 386 35 357 3.72e-27 113
Msa1433080 AT1G31450.1 28.982 383 229 16 77 439 83 442 3.75e-26 110
Msa1433080 AT3G18490.1 27.688 372 228 13 77 438 160 500 7.88e-26 110
Msa1433080 AT1G49050.3 26.935 323 197 14 40 349 168 464 1.08e-25 109
Msa1433080 AT2G28030.1 27.062 388 214 15 74 443 56 392 1.12e-25 108
Msa1433080 AT3G51360.5 29.692 357 212 16 36 376 51 384 1.17e-25 108
Msa1433080 AT3G51360.4 29.692 357 212 16 36 376 41 374 2.04e-25 108
Msa1433080 AT5G02190.1 27.979 386 224 16 88 441 82 445 3.22e-25 108
Msa1433080 AT2G28040.2 26.772 381 226 14 71 443 51 386 1.48e-24 105
Msa1433080 AT3G20015.1 28.150 373 226 16 77 438 129 470 1.58e-24 106
Msa1433080 AT2G28040.1 26.772 381 226 14 71 443 60 395 2.15e-24 105
Msa1433080 AT2G28220.1 25.301 415 263 15 36 443 28 402 4.01e-24 104
Msa1433080 AT5G45120.1 28.230 418 224 21 79 440 83 480 8.35e-24 104
Msa1433080 AT2G28010.1 25.266 376 236 12 74 443 60 396 2.25e-23 102
Msa1433080 AT3G51360.2 29.968 317 182 15 36 339 51 340 7.59e-23 99.8
Msa1433080 AT2G28225.1 25.455 385 220 17 74 439 62 398 1.02e-22 100
Msa1433080 AT3G51360.3 27.778 342 216 13 118 452 6 323 5.16e-22 97.8
Msa1433080 AT3G51360.8 27.778 342 216 13 118 452 6 323 5.16e-22 97.8
Msa1433080 AT3G59080.2 25.263 380 217 10 77 439 168 497 5.64e-22 99.0
Msa1433080 AT5G07030.1 26.913 379 231 17 74 439 110 455 1.05e-21 97.8
Msa1433080 AT2G39710.1 27.949 390 224 20 83 440 69 433 2.02e-21 97.1
Msa1433080 AT1G66180.1 23.636 385 239 13 83 442 76 430 5.99e-21 95.5
Msa1433080 AT3G52500.1 26.087 414 222 17 77 439 88 468 2.46e-20 94.0
Msa1433080 AT5G10760.1 25.776 419 256 18 38 438 83 464 5.36e-19 89.7
Msa1433080 AT5G37540.1 22.917 384 243 14 83 440 84 440 6.72e-18 86.3
Msa1433080 AT1G79720.1 25.714 385 228 16 79 440 135 484 3.09e-17 84.7
Msa1433080 AT3G25700.2 35.507 138 79 3 77 211 82 212 9.85e-16 79.0
Msa1433080 AT3G25700.2 29.286 140 91 3 305 440 214 349 1.43e-12 69.3
Msa1433080 AT4G12920.1 24.259 371 242 14 75 440 53 389 9.12e-13 70.1

Find 125 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTGTATATCTGTGATAAT+TGG 0.171336 tig0027224:+54356 Msa1433080:three_prime_UTR
AGAGCAGTTGATGGTATATT+TGG 0.176792 tig0027224:+51894 Msa1433080:CDS
GCGGCGACGGTGATTGTTTC+TGG 0.190594 tig0027224:+50356 Msa1433080:CDS
ACAACCGCCATTAATTAAAA+TGG 0.215842 tig0027224:-50305 None:intergenic
CTTTGCTGGTGGTGCATCTA+TGG 0.236164 tig0027224:+52820 Msa1433080:CDS
GTTGATGGTATATTTGGATT+TGG 0.272872 tig0027224:+51900 Msa1433080:CDS
ATATTGATTGTGTGCACAAT+AGG 0.281707 tig0027224:+54400 Msa1433080:three_prime_UTR
GGTGGAGGAATTCTGGTTCT+TGG 0.284857 tig0027224:+52011 Msa1433080:CDS
TTAGCTTTAGATGATTCTTT+AGG 0.298617 tig0027224:+54433 Msa1433080:three_prime_UTR
TCATCACAAGGGATTGCTTC+TGG 0.311244 tig0027224:+51954 Msa1433080:CDS
TAGCTCTGGTGGAGGAATTC+TGG 0.317072 tig0027224:+52004 Msa1433080:CDS
TGCTCTTACACTTTCCAGTA+TGG 0.324596 tig0027224:+51616 Msa1433080:CDS
TTGATGGTATATTTGGATTT+GGG 0.334758 tig0027224:+51901 Msa1433080:CDS
TTGAATTACCCGACTTGAAA+TGG 0.337659 tig0027224:-50533 None:intergenic
GTAACACTGATTTGCAGCTT+TGG 0.338989 tig0027224:+54261 Msa1433080:three_prime_UTR
CGTCGCCGCCGTTAACAATA+TGG 0.346244 tig0027224:-50342 None:intergenic
CGTGTCTGATACAATGCATT+TGG 0.348942 tig0027224:+51666 Msa1433080:CDS
GTTGTTGATTTCGCTGTTCA+TGG 0.357181 tig0027224:+50500 Msa1433080:CDS
CAATAGAATTCTGTTGTATA+AGG 0.358354 tig0027224:-52862 None:intergenic
GTATTCTCTCATTGTCTTAG+AGG 0.362226 tig0027224:+51978 Msa1433080:CDS
AGAGGCACCATTGTTGATTC+TGG 0.363062 tig0027224:+52362 Msa1433080:CDS
TCCACAGACAAGTGGGTTGC+AGG 0.365572 tig0027224:+51359 Msa1433080:CDS
GGTCAAGGAGTAACAATTCT+AGG 0.373274 tig0027224:+53546 Msa1433080:CDS
CTGTACGTACGGTTGTTTCC+AGG 0.377988 tig0027224:+52630 Msa1433080:CDS
CAAGTAAGTCTGAACTTTGC+TGG 0.395862 tig0027224:+52806 Msa1433080:CDS
GGTGCAGCAGTGTGGTGTAT+TGG 0.396578 tig0027224:+53507 Msa1433080:CDS
ATTTGCACATAAAATTCAAC+TGG 0.400042 tig0027224:-51285 None:intergenic
TATAAAGAAGGAACCGGTTA+AGG 0.402239 tig0027224:-50181 None:intergenic
ACACTGGAAGTGATGTTCTC+TGG 0.404650 tig0027224:+51310 Msa1433080:CDS
TAATCTCTTGTTCTGACAAA+AGG 0.408641 tig0027224:+51539 Msa1433080:CDS
TTCCGCTACCATCTCCATAC+TGG 0.415108 tig0027224:-51630 None:intergenic
AGTGGAGTATAAACTATGTT+AGG 0.415808 tig0027224:-52047 None:intergenic
CAAAATTGTTGTTTACGATC+TGG 0.415968 tig0027224:+53949 Msa1433080:CDS
TTGTCAAGTCTCCACTCTGC+TGG 0.418743 tig0027224:-51863 None:intergenic
TATTGATTGTGTGCACAATA+GGG 0.419892 tig0027224:+54401 Msa1433080:three_prime_UTR
TTCTTGCAGTCTTCTTCAAA+TGG 0.427647 tig0027224:+50476 Msa1433080:CDS
AAAACAACAACAACCTTAAC+CGG 0.429528 tig0027224:+50168 Msa1433080:five_prime_UTR
AGAGCACTGGTTGGTCTGAC+TGG 0.433140 tig0027224:-51599 None:intergenic
ATTCACCGCCATATTGTTAA+CGG 0.438617 tig0027224:+50334 Msa1433080:CDS
CCTTTGATCCATTTCAAGTC+GGG 0.440444 tig0027224:+50525 Msa1433080:CDS
GTTAATGTTTCTGCATCAAC+TGG 0.443678 tig0027224:+54139 Msa1433080:CDS
GAATCATGTGAAGTAATGAT+TGG 0.453341 tig0027224:-54477 None:intergenic
TAAGAAACATTGATTTCCCC+TGG 0.458930 tig0027224:-52648 None:intergenic
CTGGTTGGTCTGACTGGAAC+AGG 0.461396 tig0027224:-51593 None:intergenic
TGGTCGGTCAACGCATTGGA+TGG 0.464375 tig0027224:+53969 Msa1433080:CDS
ATGTTCTCTAACATCATCAT+TGG 0.471641 tig0027224:-50097 None:intergenic
CACTGGAAGTGATGTTCTCT+GGG 0.471938 tig0027224:+51311 Msa1433080:CDS
AAATCTGACAGAGCAGTTGA+TGG 0.478478 tig0027224:+51885 Msa1433080:CDS
TCTGGTTCTTGGTGAGATTG+TGG 0.485463 tig0027224:+52022 Msa1433080:CDS
ATCTCCGGCGACTCTAACGT+TGG 0.491902 tig0027224:+50379 Msa1433080:CDS
TTTAGCATACCTTGCAGAAG+AGG 0.496730 tig0027224:+52391 Msa1433080:CDS
GGAGATGGTAGCGGAACCTC+AGG 0.500285 tig0027224:+51637 Msa1433080:CDS
GGACTTGCGTCGTCAAAGAC+AGG 0.504369 tig0027224:+54223 Msa1433080:CDS
GGAAGCAATTCTTTACATGA+TGG 0.505206 tig0027224:+54202 Msa1433080:CDS
AATGTTTCTTAATTACCGAC+AGG 0.509619 tig0027224:+52660 Msa1433080:CDS
ACTGTATATAAAGAAGGAAC+CGG 0.510801 tig0027224:-50187 None:intergenic
GGTGATTGTTTCTGGATCTC+CGG 0.513289 tig0027224:+50364 Msa1433080:CDS
GAGGATGTTGATGAACTCCT+AGG 0.515381 tig0027224:-51514 None:intergenic
ATCTCACAGCTCTCATCACA+AGG 0.517730 tig0027224:+51942 Msa1433080:CDS
GTTACTACTGTATATAAAGA+AGG 0.522923 tig0027224:-50193 None:intergenic
TATTGGCTTTCAGAAAAGCC+AGG 0.524879 tig0027224:+53524 Msa1433080:CDS
GATCTGGTCGGTCAACGCAT+TGG 0.526025 tig0027224:+53965 Msa1433080:CDS
CATATTGTTAACGGCGGCGA+CGG 0.527436 tig0027224:+50343 Msa1433080:CDS
TCAAAGCTAGAGACTTGTTA+AGG 0.527714 tig0027224:+50444 Msa1433080:CDS
CTTTCCAACGTTAGAGTCGC+CGG 0.530143 tig0027224:-50383 None:intergenic
TGTACGTACGGTTGTTTCCA+GGG 0.530182 tig0027224:+52631 Msa1433080:CDS
ATGGATCGACTTGAAGTGCC+TGG 0.539684 tig0027224:-52316 None:intergenic
TACACTTTCCAGTATGGAGA+TGG 0.539809 tig0027224:+51622 Msa1433080:CDS
TGTAGGTTTAAGTTGTAATG+AGG 0.541851 tig0027224:-52275 None:intergenic
GTTTCTGCATCAACTGGCAC+TGG 0.543133 tig0027224:+54145 Msa1433080:CDS
GAGCATATCTGTCAACGGCC+AGG 0.546917 tig0027224:+52298 Msa1433080:CDS
AAACTCACCGCATTGACAAA+GGG 0.548435 tig0027224:-52422 None:intergenic
GCAGAAAGAGAGAAAGAGAG+AGG 0.549953 tig0027224:-50070 None:intergenic
TGTCTTAGAGGAGATAGCTC+TGG 0.550304 tig0027224:+51990 Msa1433080:CDS
ACCTTTGATCCATTTCAAGT+CGG 0.554751 tig0027224:+50524 Msa1433080:CDS
AGCTCAATTCCATGATTCGT+CGG 0.558726 tig0027224:-50413 None:intergenic
AATACCACAATCTGTACGTA+CGG 0.559219 tig0027224:+52619 Msa1433080:CDS
GAAACTCACCGCATTGACAA+AGG 0.562074 tig0027224:-52423 None:intergenic
CGTTGACAGATATGCTCTGT+AGG 0.566016 tig0027224:-52292 None:intergenic
CAGAAAGAGAGAAAGAGAGA+GGG 0.572348 tig0027224:-50069 None:intergenic
AACGGTTGTCCACAGACAAG+TGG 0.572605 tig0027224:+51351 Msa1433080:CDS
ACCTGCAACCCACTTGTCTG+TGG 0.577779 tig0027224:-51360 None:intergenic
GGTCGGTCAACGCATTGGAT+GGG 0.580910 tig0027224:+53970 Msa1433080:CDS
CCCGACTTGAAATGGATCAA+AGG 0.582246 tig0027224:-50525 None:intergenic
AGTGGAAATAGAACATACAT+AGG 0.583122 tig0027224:-54337 None:intergenic
GAATTGTTACTCCTTGACCC+TGG 0.585059 tig0027224:-53542 None:intergenic
TGCACATAAAATTCAACTGG+AGG 0.588358 tig0027224:-51282 None:intergenic
ACTGGAAAGTGTAAGAGCAC+TGG 0.588565 tig0027224:-51612 None:intergenic
ATTGTTGTTTACGATCTGGT+CGG 0.593128 tig0027224:+53953 Msa1433080:CDS
GTCAGTTGTAGCTCTTGCAA+CGG 0.594137 tig0027224:+51333 Msa1433080:CDS
GCTAGAGACTTGTTAAGGCA+TGG 0.596247 tig0027224:+50449 Msa1433080:CDS
AGAAGTGAATACGTGAATGC+TGG 0.597995 tig0027224:+54169 Msa1433080:CDS
TTTCAGAAAAGCCAGGGTCA+AGG 0.603671 tig0027224:+53531 Msa1433080:CDS
TTCCAGTATGGAGATGGTAG+CGG 0.610454 tig0027224:+51628 Msa1433080:CDS
ACAACCGTACGTACAGATTG+TGG 0.610972 tig0027224:-52623 None:intergenic
GATCATAAGCCTCTTCTGCA+AGG 0.611239 tig0027224:-52400 None:intergenic
CTTAGAGGAGATAGCTCTGG+TGG 0.612995 tig0027224:+51993 Msa1433080:CDS
AGCTGAAGAATTTGTAGACA+CGG 0.613446 tig0027224:-51707 None:intergenic
GCTTACTGTGATGGAACAAG+TGG 0.613732 tig0027224:-52065 None:intergenic
AGGATGTTGATGAACTCCTA+GGG 0.614361 tig0027224:-51513 None:intergenic
TACGTGAATGCTGGAGAGAT+AGG 0.622109 tig0027224:+54178 Msa1433080:CDS
GTACGTACGGTTGTTTCCAG+GGG 0.623948 tig0027224:+52632 Msa1433080:CDS
TCTGACAAAAGGTGCAACAG+TGG 0.632626 tig0027224:+51550 Msa1433080:CDS
GCAAAGTTCAGACTTACTTG+AGG 0.643364 tig0027224:-52803 None:intergenic
TTTGCAACATCAAGCAACAG+AGG 0.646159 tig0027224:+52344 Msa1433080:CDS
GTGAATGCTGGAGAGATAGG+TGG 0.646271 tig0027224:+54181 Msa1433080:CDS
ATTGGCTTTCAGAAAAGCCA+GGG 0.649058 tig0027224:+53525 Msa1433080:CDS
AGAGGAGATAGCTCTGGTGG+AGG 0.653271 tig0027224:+51996 Msa1433080:CDS
CTACAGAGCATATCTGTCAA+CGG 0.662152 tig0027224:+52293 Msa1433080:CDS
GAAAGTGTAAGAGCACTGGT+TGG 0.666621 tig0027224:-51608 None:intergenic
TCAATATTGAAAATAACATG+TGG 0.667687 tig0027224:-54384 None:intergenic
CGCCGCCGTTAACAATATGG+CGG 0.668764 tig0027224:-50339 None:intergenic
CAATTATCACAGATATACAG+TGG 0.670628 tig0027224:-54355 None:intergenic
TGTTGTATAAGGTAGTCCTG+TGG 0.671726 tig0027224:-52851 None:intergenic
ACGGTTGTCCACAGACAAGT+GGG 0.671786 tig0027224:+51352 Msa1433080:CDS
AGTTGTTCCAGAATCAACAA+TGG 0.673134 tig0027224:-52369 None:intergenic
CACCGCCATATTGTTAACGG+CGG 0.679075 tig0027224:+50337 Msa1433080:CDS
TTATGATCCCTTTGTCAATG+CGG 0.686569 tig0027224:+52415 Msa1433080:CDS
ATCAGACACGTAATAGCCTG+AGG 0.690351 tig0027224:-51653 None:intergenic
TACAGGGTGGTGCAGCAGTG+TGG 0.694411 tig0027224:+53499 Msa1433080:intron
GTAAGTCTGAACTTTGCTGG+TGG 0.697231 tig0027224:+52809 Msa1433080:CDS
AGTTGTAGTAACCAGCAGAG+TGG 0.699553 tig0027224:+51852 Msa1433080:intron
CAAGGAGTAACAATTCTAGG+AGG 0.710587 tig0027224:+53549 Msa1433080:CDS
GTCAGAACAAGAGATTAACG+AGG 0.724638 tig0027224:-51533 None:intergenic
TCTCACAGCTCTCATCACAA+GGG 0.756077 tig0027224:+51943 Msa1433080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAACCATTTTAATTAA+TGG + tig0027224:50298-50317 Msa1433080:exon 10.0%
!!! AATTTCACTTTTTTGATAAA+GGG + tig0027224:50559-50578 Msa1433080:intron 15.0%
!! ATCTATAATTCTTCAATTTA+TGG + tig0027224:50690-50709 Msa1433080:intron 15.0%
!!! TCAATTTTGTTTTCTATTTT+TGG + tig0027224:50870-50889 Msa1433080:intron 15.0%
!!! AATAGCAATTTTATTTTCAA+GGG - tig0027224:52090-52109 None:intergenic 15.0%
!!! TAATAGCAATTTTATTTTCA+AGG - tig0027224:52091-52110 None:intergenic 15.0%
!!! TAACTTAAAAGTATTATCTT+TGG - tig0027224:52953-52972 None:intergenic 15.0%
!! TAAAATCAAAATCAGATTAA+TGG - tig0027224:53024-53043 None:intergenic 15.0%
!! ATTTGCAATTCTAATATATT+TGG + tig0027224:53171-53190 Msa1433080:intron 15.0%
!!! TGTATATCATTGTTTTTAAT+GGG - tig0027224:53782-53801 None:intergenic 15.0%
!!! TAAACCATTTTAATTAATGG+CGG + tig0027224:50301-50320 Msa1433080:exon 20.0%
!!! GAATTTCACTTTTTTGATAA+AGG + tig0027224:50558-50577 Msa1433080:intron 20.0%
!!! TTTTTAGGATTTTTGAGTAA+TGG + tig0027224:50643-50662 Msa1433080:intron 20.0%
!!! TGTGTTTGATTTAAAATTGA+TGG + tig0027224:51218-51237 Msa1433080:intron 20.0%
!! ATATAATGGTTTACTGTTTA+TGG + tig0027224:51458-51477 Msa1433080:intron 20.0%
!!! TTATTGGTTTAATCTAATCT+CGG + tig0027224:51805-51824 Msa1433080:intron 20.0%
!! AATCGTAAAAAATGTACAAA+TGG - tig0027224:52703-52722 None:intergenic 20.0%
!!! TTTTCTTTTTCATTTTTCTG+AGG + tig0027224:52905-52924 Msa1433080:intron 20.0%
!! ATTGAACGAATCATTTATTA+AGG + tig0027224:53106-53125 Msa1433080:intron 20.0%
!! AAAAAGTAATAGATTTCAGA+TGG - tig0027224:53307-53326 None:intergenic 20.0%
!! ATCAACAATATCTAAGAATT+TGG - tig0027224:53370-53389 None:intergenic 20.0%
!! AAATAAATGCATGTAATCAA+AGG - tig0027224:53394-53413 None:intergenic 20.0%
!! AAACATTTCCTTCTAAATAT+TGG - tig0027224:53613-53632 None:intergenic 20.0%
!!! ATTTTAAATCCCAATAGTAA+TGG - tig0027224:53740-53759 None:intergenic 20.0%
!!! GTGTATATCATTGTTTTTAA+TGG - tig0027224:53783-53802 None:intergenic 20.0%
!! TTGTTCATAAATAATCTGTT+AGG + tig0027224:53832-53851 Msa1433080:intron 20.0%
!! TGTTCATAAATAATCTGTTA+GGG + tig0027224:53833-53852 Msa1433080:intron 20.0%
!!! TGTTTTTAATTAAACAATGC+AGG + tig0027224:54106-54125 Msa1433080:intron 20.0%
!! TCAATATTGAAAATAACATG+TGG - tig0027224:54387-54406 None:intergenic 20.0%
! TTGTAGTGTGACAAAAAATT+TGG - tig0027224:50042-50061 None:intergenic 25.0%
! GTTACTACTGTATATAAAGA+AGG - tig0027224:50196-50215 None:intergenic 25.0%
!!! GGTTTTACTTTTCATGTTTT+TGG + tig0027224:50584-50603 Msa1433080:intron 25.0%
!!! TGAATGCTTTTTGGATTTTT+AGG + tig0027224:50628-50647 Msa1433080:intron 25.0%
!!! TAGTTTAGTTTAGTTCTCTA+TGG + tig0027224:50837-50856 Msa1433080:intron 25.0%
! TAGTTTCTTTAAGTGATGAT+AGG + tig0027224:50904-50923 Msa1433080:intron 25.0%
! CACTTATAGAAACCATATAT+TGG - tig0027224:50930-50949 None:intergenic 25.0%
! AGATAAGATATAATGCAAAG+AGG - tig0027224:51179-51198 None:intergenic 25.0%
! ATTTGCACATAAAATTCAAC+TGG - tig0027224:51288-51307 None:intergenic 25.0%
! GATGGAATATGTCAATATAA+TGG + tig0027224:51444-51463 Msa1433080:intron 25.0%
!!! CATTTGGATACTATTTTTGA+GGG + tig0027224:51682-51701 Msa1433080:CDS 25.0%
!!! TTTTTTTCCTTCGTGTTTAT+TGG + tig0027224:51789-51808 Msa1433080:intron 25.0%
!! TTGATGGTATATTTGGATTT+GGG + tig0027224:51901-51920 Msa1433080:CDS 25.0%
! TACTTAAGTTAACGAAAAGA+AGG - tig0027224:52120-52139 None:intergenic 25.0%
!! AACCCATTTTCAAATATTTC+AGG + tig0027224:52252-52271 Msa1433080:intron 25.0%
!!! CATTAACATTATGTTGCTTT+AGG + tig0027224:52477-52496 Msa1433080:intron 25.0%
!!! GTACATTTTTTACGATTATG+TGG + tig0027224:52706-52725 Msa1433080:intron 25.0%
! ACTAAACACAATAATACGAA+AGG - tig0027224:52768-52787 None:intergenic 25.0%
! CAATAGAATTCTGTTGTATA+AGG - tig0027224:52865-52884 None:intergenic 25.0%
!!! TTTTTCATTTTTCTGAGGAT+TGG + tig0027224:52910-52929 Msa1433080:intron 25.0%
! ATGTAAAATGTCGTAAAGAA+AGG - tig0027224:53073-53092 None:intergenic 25.0%
! GTAGTACTTGAGAATAATTT+CGG - tig0027224:53144-53163 None:intergenic 25.0%
! ATAGATTGCAAGGATAATAA+AGG - tig0027224:53207-53226 None:intergenic 25.0%
!!! TTACTTTTTAGTGTAGAGAA+AGG + tig0027224:53318-53337 Msa1433080:intron 25.0%
!!! CTAAATTTTTGAGTTCAAAG+TGG - tig0027224:53582-53601 None:intergenic 25.0%
! ATCATGAACCAATATTTAGA+AGG + tig0027224:53602-53621 Msa1433080:intron 25.0%
! TCATACTAAAAGTTTATACG+TGG - tig0027224:53660-53679 None:intergenic 25.0%
!!! TAGTAATGGTGATCATTTTT+TGG - tig0027224:53726-53745 None:intergenic 25.0%
! AAAATGATCACCATTACTAT+TGG + tig0027224:53727-53746 Msa1433080:intron 25.0%
! AAATGATCACCATTACTATT+GGG + tig0027224:53728-53747 Msa1433080:intron 25.0%
! AAATTCACTATATGTCTTTC+TGG - tig0027224:54027-54046 None:intergenic 25.0%
! GACATATAGTGAATTTCATA+TGG + tig0027224:54031-54050 Msa1433080:intron 25.0%
!!! TTAGCTTTAGATGATTCTTT+AGG + tig0027224:54433-54452 Msa1433080:three_prime_UTR 25.0%
ATGTTCTCTAACATCATCAT+TGG - tig0027224:50100-50119 None:intergenic 30.0%
!! TTTTGTTTGGACAAGAGTTA+AGG - tig0027224:50151-50170 None:intergenic 30.0%
!!! AGGTTGTTGTTGTTTTTGTT+TGG - tig0027224:50164-50183 None:intergenic 30.0%
AAAACAACAACAACCTTAAC+CGG + tig0027224:50168-50187 Msa1433080:five_prime_UTR 30.0%
ACTGTATATAAAGAAGGAAC+CGG - tig0027224:50190-50209 None:intergenic 30.0%
ACAACCGCCATTAATTAAAA+TGG - tig0027224:50308-50327 None:intergenic 30.0%
!!! TCACTTTTTTGATAAAGGGT+TGG + tig0027224:50563-50582 Msa1433080:intron 30.0%
!!! GAATCAAGTTGAATGCTTTT+TGG + tig0027224:50619-50638 Msa1433080:intron 30.0%
CACTCTAAACTGATCATAAA+AGG - tig0027224:50744-50763 None:intergenic 30.0%
! AGAAAGCAATTTAGAAGTGT+TGG - tig0027224:51058-51077 None:intergenic 30.0%
TGTAAGCAAAACTAACTTGA+AGG - tig0027224:51087-51106 None:intergenic 30.0%
!! TAAAATTGATGGACTATTGC+AGG + tig0027224:51229-51248 Msa1433080:intron 30.0%
!! TATTTTACAAAGGTGCAGTT+AGG + tig0027224:51255-51274 Msa1433080:CDS 30.0%
! TTTTATGTGCAAATTGACAC+TGG + tig0027224:51294-51313 Msa1433080:CDS 30.0%
TAATCTCTTGTTCTGACAAA+AGG + tig0027224:51539-51558 Msa1433080:CDS 30.0%
!!! GCATTTGGATACTATTTTTG+AGG + tig0027224:51681-51700 Msa1433080:CDS 30.0%
GATTAAACCAATAAACACGA+AGG - tig0027224:51799-51818 None:intergenic 30.0%
!! GTTGATGGTATATTTGGATT+TGG + tig0027224:51900-51919 Msa1433080:CDS 30.0%
AGTGGAGTATAAACTATGTT+AGG - tig0027224:52050-52069 None:intergenic 30.0%
TTAACATTTGATTATGCTCG+TGG - tig0027224:52217-52236 None:intergenic 30.0%
GGCCTGAAATATTTGAAAAT+GGG - tig0027224:52257-52276 None:intergenic 30.0%
AGGCCTGAAATATTTGAAAA+TGG - tig0027224:52258-52277 None:intergenic 30.0%
!! TGTAGGTTTAAGTTGTAATG+AGG - tig0027224:52278-52297 None:intergenic 30.0%
! TAGCAATCATGTCATGTTTT+TGG + tig0027224:52530-52549 Msa1433080:intron 30.0%
GGAGATTTATCAAACATTGA+TGG + tig0027224:52560-52579 Msa1433080:intron 30.0%
AATGTTTCTTAATTACCGAC+AGG + tig0027224:52660-52679 Msa1433080:CDS 30.0%
ATAAAAACATCCTAACCTGT+CGG - tig0027224:52678-52697 None:intergenic 30.0%
! CTTTACGACATTTTACATGA+TGG + tig0027224:53075-53094 Msa1433080:intron 30.0%
CGAATCATTTATTAAGGCAT+AGG + tig0027224:53112-53131 Msa1433080:intron 30.0%
AACTAAACAGATAGATTGCA+AGG - tig0027224:53217-53236 None:intergenic 30.0%
GCATTTAGTGAGAAAACATA+CGG - tig0027224:53459-53478 None:intergenic 30.0%
! TGTTTTCTCACTAAATGCTA+CGG + tig0027224:53461-53480 Msa1433080:intron 30.0%
TAGTTGTATAACACTCTTGA+AGG - tig0027224:53870-53889 None:intergenic 30.0%
!! TCTTTGACTTCTTTGTGTTT+TGG + tig0027224:53907-53926 Msa1433080:intron 30.0%
!! CAAAATTGTTGTTTACGATC+TGG + tig0027224:53949-53968 Msa1433080:CDS 30.0%
TAGAATCTAATGCAATGTCA+AGG - tig0027224:54079-54098 None:intergenic 30.0%
AGTGGAAATAGAACATACAT+AGG - tig0027224:54340-54359 None:intergenic 30.0%
CAATTATCACAGATATACAG+TGG - tig0027224:54358-54377 None:intergenic 30.0%
CACTGTATATCTGTGATAAT+TGG + tig0027224:54356-54375 Msa1433080:three_prime_UTR 30.0%
! ATATTGATTGTGTGCACAAT+AGG + tig0027224:54400-54419 Msa1433080:three_prime_UTR 30.0%
! TATTGATTGTGTGCACAATA+GGG + tig0027224:54401-54420 Msa1433080:three_prime_UTR 30.0%
GAATCATGTGAAGTAATGAT+TGG - tig0027224:54480-54499 None:intergenic 30.0%
TATAAAGAAGGAACCGGTTA+AGG - tig0027224:50184-50203 None:intergenic 35.0%
ATTCACCGCCATATTGTTAA+CGG + tig0027224:50334-50353 Msa1433080:CDS 35.0%
!! TCAAAGCTAGAGACTTGTTA+AGG + tig0027224:50444-50463 Msa1433080:CDS 35.0%
TTCTTGCAGTCTTCTTCAAA+TGG + tig0027224:50476-50495 Msa1433080:CDS 35.0%
! ACCTTTGATCCATTTCAAGT+CGG + tig0027224:50524-50543 Msa1433080:CDS 35.0%
! TTGAATTACCCGACTTGAAA+TGG - tig0027224:50536-50555 None:intergenic 35.0%
AGTGATGATAGGCCAATATA+TGG + tig0027224:50915-50934 Msa1433080:intron 35.0%
AAAGATAAGCTGCACTAAGA+TGG - tig0027224:51155-51174 None:intergenic 35.0%
! TTGCAGGCTCTATTTTACAA+AGG + tig0027224:51245-51264 Msa1433080:intron 35.0%
TGCACATAAAATTCAACTGG+AGG - tig0027224:51285-51304 None:intergenic 35.0%
AACAGTGAACAATGTAGAGA+TGG + tig0027224:51426-51445 Msa1433080:intron 35.0%
AGCTGAAGAATTTGTAGACA+CGG - tig0027224:51710-51729 None:intergenic 35.0%
!! AGAGCAGTTGATGGTATATT+TGG + tig0027224:51894-51913 Msa1433080:CDS 35.0%
GTATTCTCTCATTGTCTTAG+AGG + tig0027224:51978-51997 Msa1433080:CDS 35.0%
AATTGAGATTGCTCTCATCA+TGG - tig0027224:52144-52163 None:intergenic 35.0%
AGATCTGTGTGTTTGTTCTT+AGG + tig0027224:52190-52209 Msa1433080:intron 35.0%
CTTGATGTTGCAAAAACTGA+TGG - tig0027224:52338-52357 None:intergenic 35.0%
AGTTGTTCCAGAATCAACAA+TGG - tig0027224:52372-52391 None:intergenic 35.0%
TTATGATCCCTTTGTCAATG+CGG + tig0027224:52415-52434 Msa1433080:CDS 35.0%
!!! ATCATGTCATGTTTTTGGCT+TGG + tig0027224:52535-52554 Msa1433080:intron 35.0%
AATACCACAATCTGTACGTA+CGG + tig0027224:52619-52638 Msa1433080:CDS 35.0%
!! TAAGAAACATTGATTTCCCC+TGG - tig0027224:52651-52670 None:intergenic 35.0%
TTCTTAATTACCGACAGGTT+AGG + tig0027224:52665-52684 Msa1433080:intron 35.0%
!!! ATCTATGGTTTTAAGACCAC+AGG + tig0027224:52835-52854 Msa1433080:CDS 35.0%
!!! CATTTTTCTGAGGATTGGTT+TGG + tig0027224:52915-52934 Msa1433080:intron 35.0%
AGCTTATGCTTCACTTTGTT+TGG + tig0027224:53423-53442 Msa1433080:intron 35.0%
!! ATTGTTGTTTACGATCTGGT+CGG + tig0027224:53953-53972 Msa1433080:CDS 35.0%
! GTTAATGTTTCTGCATCAAC+TGG + tig0027224:54139-54158 Msa1433080:CDS 35.0%
GGAAGCAATTCTTTACATGA+TGG + tig0027224:54202-54221 Msa1433080:CDS 35.0%
CAGAAAGAGAGAAAGAGAGA+GGG - tig0027224:50072-50091 None:intergenic 40.0%
AGCTCAATTCCATGATTCGT+CGG - tig0027224:50416-50435 None:intergenic 40.0%
!! GTTGTTGATTTCGCTGTTCA+TGG + tig0027224:50500-50519 Msa1433080:CDS 40.0%
! CCTTTGATCCATTTCAAGTC+GGG + tig0027224:50525-50544 Msa1433080:CDS 40.0%
GCATCAACACTTCAGATTGA+AGG + tig0027224:50992-51011 Msa1433080:intron 40.0%
! ACTTCAGATTGAAGGTGTGT+CGG + tig0027224:51000-51019 Msa1433080:intron 40.0%
! ATTTGCGTAAGTGTCGTGTT+GGG + tig0027224:51126-51145 Msa1433080:intron 40.0%
!!! AGCTCAATTTTTTCGACCCT+AGG + tig0027224:51497-51516 Msa1433080:CDS 40.0%
! AGGATGTTGATGAACTCCTA+GGG - tig0027224:51516-51535 None:intergenic 40.0%
GTCAGAACAAGAGATTAACG+AGG - tig0027224:51536-51555 None:intergenic 40.0%
TGCTCTTACACTTTCCAGTA+TGG + tig0027224:51616-51635 Msa1433080:CDS 40.0%
TACACTTTCCAGTATGGAGA+TGG + tig0027224:51622-51641 Msa1433080:CDS 40.0%
CGTGTCTGATACAATGCATT+TGG + tig0027224:51666-51685 Msa1433080:CDS 40.0%
! TCTTCAGCTCCTGTAGTTTT+TGG + tig0027224:51721-51740 Msa1433080:CDS 40.0%
! CTTCAGCTCCTGTAGTTTTT+GGG + tig0027224:51722-51741 Msa1433080:CDS 40.0%
AAAGCTCACCCAAAAACTAC+AGG - tig0027224:51733-51752 None:intergenic 40.0%
GAGCTTTCATGAGCATGAAA+TGG + tig0027224:51746-51765 Msa1433080:intron 40.0%
CTGCTCGATTCACAAAGTAA+CGG - tig0027224:51834-51853 None:intergenic 40.0%
AAATCTGACAGAGCAGTTGA+TGG + tig0027224:51885-51904 Msa1433080:CDS 40.0%
!! TTTTCAAGGGCTTACTGTGA+TGG - tig0027224:52077-52096 None:intergenic 40.0%
CTACAGAGCATATCTGTCAA+CGG + tig0027224:52293-52312 Msa1433080:CDS 40.0%
TTTGCAACATCAAGCAACAG+AGG + tig0027224:52344-52363 Msa1433080:CDS 40.0%
TTTAGCATACCTTGCAGAAG+AGG + tig0027224:52391-52410 Msa1433080:CDS 40.0%
! AAACTCACCGCATTGACAAA+GGG - tig0027224:52425-52444 None:intergenic 40.0%
! TCAATGCGGTGAGTTTCTTT+TGG + tig0027224:52429-52448 Msa1433080:intron 40.0%
! CAATGCGGTGAGTTTCTTTT+GGG + tig0027224:52430-52449 Msa1433080:intron 40.0%
!!! TGTCATGTTTTTGGCTTGGT+TGG + tig0027224:52539-52558 Msa1433080:intron 40.0%
GCAAAGTTCAGACTTACTTG+AGG - tig0027224:52806-52825 None:intergenic 40.0%
CAAGTAAGTCTGAACTTTGC+TGG + tig0027224:52806-52825 Msa1433080:CDS 40.0%
TGTTGTATAAGGTAGTCCTG+TGG - tig0027224:52854-52873 None:intergenic 40.0%
AAGTTAGAGTGAGTTGCAGT+TGG + tig0027224:52967-52986 Msa1433080:intron 40.0%
! GTCGTAAAGAAAGGCACATT+AGG - tig0027224:53064-53083 None:intergenic 40.0%
GTTTAGTTGAGTGCATTAGC+AGG + tig0027224:53229-53248 Msa1433080:intron 40.0%
GTGAACCTATGCATGTTACA+GGG + tig0027224:53483-53502 Msa1433080:intron 40.0%
!! TATTGGCTTTCAGAAAAGCC+AGG + tig0027224:53524-53543 Msa1433080:CDS 40.0%
!! ATTGGCTTTCAGAAAAGCCA+GGG + tig0027224:53525-53544 Msa1433080:CDS 40.0%
GGTCAAGGAGTAACAATTCT+AGG + tig0027224:53546-53565 Msa1433080:CDS 40.0%
CAAGGAGTAACAATTCTAGG+AGG + tig0027224:53549-53568 Msa1433080:CDS 40.0%
AGAAGTGAATACGTGAATGC+TGG + tig0027224:54169-54188 Msa1433080:CDS 40.0%
!! GTAACACTGATTTGCAGCTT+TGG + tig0027224:54261-54280 Msa1433080:three_prime_UTR 40.0%
GCAGAAAGAGAGAAAGAGAG+AGG - tig0027224:50073-50092 None:intergenic 45.0%
!! GGTGATTGTTTCTGGATCTC+CGG + tig0027224:50364-50383 Msa1433080:CDS 45.0%
! AGAGCTTTTCCGACGAATCA+TGG + tig0027224:50404-50423 Msa1433080:CDS 45.0%
!! GCTAGAGACTTGTTAAGGCA+TGG + tig0027224:50449-50468 Msa1433080:CDS 45.0%
! CCCGACTTGAAATGGATCAA+AGG - tig0027224:50528-50547 None:intergenic 45.0%
! GATTTGCGTAAGTGTCGTGT+TGG + tig0027224:51125-51144 Msa1433080:intron 45.0%
ACACTGGAAGTGATGTTCTC+TGG + tig0027224:51310-51329 Msa1433080:CDS 45.0%
CACTGGAAGTGATGTTCTCT+GGG + tig0027224:51311-51330 Msa1433080:CDS 45.0%
GTCAGTTGTAGCTCTTGCAA+CGG + tig0027224:51333-51352 Msa1433080:CDS 45.0%
! GAGGATGTTGATGAACTCCT+AGG - tig0027224:51517-51536 None:intergenic 45.0%
! TCTGACAAAAGGTGCAACAG+TGG + tig0027224:51550-51569 Msa1433080:CDS 45.0%
!! GAAAGTGTAAGAGCACTGGT+TGG - tig0027224:51611-51630 None:intergenic 45.0%
! ACTGGAAAGTGTAAGAGCAC+TGG - tig0027224:51615-51634 None:intergenic 45.0%
TTCCAGTATGGAGATGGTAG+CGG + tig0027224:51628-51647 Msa1433080:CDS 45.0%
ATCAGACACGTAATAGCCTG+AGG - tig0027224:51656-51675 None:intergenic 45.0%
AGTTGTAGTAACCAGCAGAG+TGG + tig0027224:51852-51871 Msa1433080:intron 45.0%
ATCTCACAGCTCTCATCACA+AGG + tig0027224:51942-51961 Msa1433080:CDS 45.0%
TCTCACAGCTCTCATCACAA+GGG + tig0027224:51943-51962 Msa1433080:CDS 45.0%
TCATCACAAGGGATTGCTTC+TGG + tig0027224:51954-51973 Msa1433080:CDS 45.0%
TGTCTTAGAGGAGATAGCTC+TGG + tig0027224:51990-52009 Msa1433080:CDS 45.0%
!! TCTGGTTCTTGGTGAGATTG+TGG + tig0027224:52022-52041 Msa1433080:CDS 45.0%
! GCTTACTGTGATGGAACAAG+TGG - tig0027224:52068-52087 None:intergenic 45.0%
CGTTGACAGATATGCTCTGT+AGG - tig0027224:52295-52314 None:intergenic 45.0%
!! AGAGGCACCATTGTTGATTC+TGG + tig0027224:52362-52381 Msa1433080:CDS 45.0%
GATCATAAGCCTCTTCTGCA+AGG - tig0027224:52403-52422 None:intergenic 45.0%
! GAAACTCACCGCATTGACAA+AGG - tig0027224:52426-52445 None:intergenic 45.0%
ACAACCGTACGTACAGATTG+TGG - tig0027224:52626-52645 None:intergenic 45.0%
! TGTACGTACGGTTGTTTCCA+GGG + tig0027224:52631-52650 Msa1433080:CDS 45.0%
GTAAGTCTGAACTTTGCTGG+TGG + tig0027224:52809-52828 Msa1433080:CDS 45.0%
GGTGAACCTATGCATGTTAC+AGG + tig0027224:53482-53501 Msa1433080:intron 45.0%
AACCTATGCATGTTACAGGG+TGG + tig0027224:53486-53505 Msa1433080:intron 45.0%
TTTCAGAAAAGCCAGGGTCA+AGG + tig0027224:53531-53550 Msa1433080:CDS 45.0%
GAATTGTTACTCCTTGACCC+TGG - tig0027224:53545-53564 None:intergenic 45.0%
CAAGAAGGTAAAACAGGAGG+AGG - tig0027224:53691-53710 None:intergenic 45.0%
!!! ATCATTTTTTGGCCGAAGGC+GGG - tig0027224:53715-53734 None:intergenic 45.0%
!!! GATCATTTTTTGGCCGAAGG+CGG - tig0027224:53716-53735 None:intergenic 45.0%
!!! GGTGATCATTTTTTGGCCGA+AGG - tig0027224:53719-53738 None:intergenic 45.0%
TACGTGAATGCTGGAGAGAT+AGG + tig0027224:54178-54197 Msa1433080:CDS 45.0%
! GTGTGCACAATAGGGTTTTC+AGG + tig0027224:54409-54428 Msa1433080:three_prime_UTR 45.0%
CACCGCCATATTGTTAACGG+CGG + tig0027224:50337-50356 Msa1433080:CDS 50.0%
CATATTGTTAACGGCGGCGA+CGG + tig0027224:50343-50362 Msa1433080:CDS 50.0%
CTTTCCAACGTTAGAGTCGC+CGG - tig0027224:50386-50405 None:intergenic 50.0%
AACGGTTGTCCACAGACAAG+TGG + tig0027224:51351-51370 Msa1433080:CDS 50.0%
ACGGTTGTCCACAGACAAGT+GGG + tig0027224:51352-51371 Msa1433080:CDS 50.0%
TTCCGCTACCATCTCCATAC+TGG - tig0027224:51633-51652 None:intergenic 50.0%
TTGTCAAGTCTCCACTCTGC+TGG - tig0027224:51866-51885 None:intergenic 50.0%
!! CTTAGAGGAGATAGCTCTGG+TGG + tig0027224:51993-52012 Msa1433080:CDS 50.0%
!! TAGCTCTGGTGGAGGAATTC+TGG + tig0027224:52004-52023 Msa1433080:CDS 50.0%
!! GGTGGAGGAATTCTGGTTCT+TGG + tig0027224:52011-52030 Msa1433080:CDS 50.0%
!! ATGGATCGACTTGAAGTGCC+TGG - tig0027224:52319-52338 None:intergenic 50.0%
! CTGTACGTACGGTTGTTTCC+AGG + tig0027224:52630-52649 Msa1433080:CDS 50.0%
! GTACGTACGGTTGTTTCCAG+GGG + tig0027224:52632-52651 Msa1433080:CDS 50.0%
! CTTTGCTGGTGGTGCATCTA+TGG + tig0027224:52820-52839 Msa1433080:CDS 50.0%
CACCACCCTGTAACATGCAT+AGG - tig0027224:53491-53510 None:intergenic 50.0%
GGGCAAGAAGGTAAAACAGG+AGG - tig0027224:53694-53713 None:intergenic 50.0%
!!! TCATTTTTTGGCCGAAGGCG+GGG - tig0027224:53714-53733 None:intergenic 50.0%
GTTTCTGCATCAACTGGCAC+TGG + tig0027224:54145-54164 Msa1433080:CDS 50.0%
GTGAATGCTGGAGAGATAGG+TGG + tig0027224:54181-54200 Msa1433080:CDS 50.0%
CGCCGCCGTTAACAATATGG+CGG - tig0027224:50342-50361 None:intergenic 55.0%
CGTCGCCGCCGTTAACAATA+TGG - tig0027224:50345-50364 None:intergenic 55.0%
ATCTCCGGCGACTCTAACGT+TGG + tig0027224:50379-50398 Msa1433080:CDS 55.0%
!! TCCACAGACAAGTGGGTTGC+AGG + tig0027224:51359-51378 Msa1433080:CDS 55.0%
ACCTGCAACCCACTTGTCTG+TGG - tig0027224:51363-51382 None:intergenic 55.0%
!! CTGGTTGGTCTGACTGGAAC+AGG - tig0027224:51596-51615 None:intergenic 55.0%
!! AGAGCACTGGTTGGTCTGAC+TGG - tig0027224:51602-51621 None:intergenic 55.0%
!! AGAGGAGATAGCTCTGGTGG+AGG + tig0027224:51996-52015 Msa1433080:CDS 55.0%
GAGCATATCTGTCAACGGCC+AGG + tig0027224:52298-52317 Msa1433080:CDS 55.0%
GGTGCAGCAGTGTGGTGTAT+TGG + tig0027224:53507-53526 Msa1433080:CDS 55.0%
GCGGGGCAAGAAGGTAAAAC+AGG - tig0027224:53697-53716 None:intergenic 55.0%
TTACCTTCTTGCCCCGCCTT+CGG + tig0027224:53700-53719 Msa1433080:intron 55.0%
GATCTGGTCGGTCAACGCAT+TGG + tig0027224:53965-53984 Msa1433080:CDS 55.0%
TGGTCGGTCAACGCATTGGA+TGG + tig0027224:53969-53988 Msa1433080:CDS 55.0%
GGTCGGTCAACGCATTGGAT+GGG + tig0027224:53970-53989 Msa1433080:CDS 55.0%
GGACTTGCGTCGTCAAAGAC+AGG + tig0027224:54223-54242 Msa1433080:CDS 55.0%
GCGGCGACGGTGATTGTTTC+TGG + tig0027224:50356-50375 Msa1433080:CDS 60.0%
!! GGAGATGGTAGCGGAACCTC+AGG + tig0027224:51637-51656 Msa1433080:CDS 60.0%
TACAGGGTGGTGCAGCAGTG+TGG + tig0027224:53499-53518 Msa1433080:intron 60.0%
TGGCCGAAGGCGGGGCAAGA+AGG - tig0027224:53706-53725 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0027224 gene 50033 54545 50033 ID=Msa1433080;Name=Msa1433080
tig0027224 mRNA 50033 54545 50033 ID=Msa1433080-mRNA-1;Parent=Msa1433080;Name=Msa1433080-mRNA-1;_AED=0.17;_eAED=0.17;_QI=284|1|1|1|1|1|10|293|478
tig0027224 exon 50033 50546 50033 ID=Msa1433080-mRNA-1:exon:8435;Parent=Msa1433080-mRNA-1
tig0027224 exon 51251 51380 51251 ID=Msa1433080-mRNA-1:exon:8436;Parent=Msa1433080-mRNA-1
tig0027224 exon 51493 51743 51493 ID=Msa1433080-mRNA-1:exon:8437;Parent=Msa1433080-mRNA-1
tig0027224 exon 51854 52081 51854 ID=Msa1433080-mRNA-1:exon:8438;Parent=Msa1433080-mRNA-1
tig0027224 exon 52274 52436 52274 ID=Msa1433080-mRNA-1:exon:8439;Parent=Msa1433080-mRNA-1
tig0027224 exon 52608 52681 52608 ID=Msa1433080-mRNA-1:exon:8440;Parent=Msa1433080-mRNA-1
tig0027224 exon 52787 52883 52787 ID=Msa1433080-mRNA-1:exon:8441;Parent=Msa1433080-mRNA-1
tig0027224 exon 53504 53570 53504 ID=Msa1433080-mRNA-1:exon:8442;Parent=Msa1433080-mRNA-1
tig0027224 exon 53933 54004 53933 ID=Msa1433080-mRNA-1:exon:8443;Parent=Msa1433080-mRNA-1
tig0027224 exon 54128 54545 54128 ID=Msa1433080-mRNA-1:exon:8444;Parent=Msa1433080-mRNA-1
tig0027224 five_prime_UTR 50033 50316 50033 ID=Msa1433080-mRNA-1:five_prime_utr;Parent=Msa1433080-mRNA-1
tig0027224 CDS 50317 50546 50317 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 51251 51380 51251 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 51493 51743 51493 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 51854 52081 51854 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 52274 52436 52274 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 52608 52681 52608 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 52787 52883 52787 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 53504 53570 53504 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 53933 54004 53933 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 CDS 54128 54252 54128 ID=Msa1433080-mRNA-1:cds;Parent=Msa1433080-mRNA-1
tig0027224 three_prime_UTR 54253 54545 54253 ID=Msa1433080-mRNA-1:three_prime_utr;Parent=Msa1433080-mRNA-1
Gene Sequence

>Msa1433080

ATGGCGGTTGTTTTTATATTCACCGCCATATTGTTAACGGCGGCGACGGTGATTGTTTCTGGATCTCCGGCGACTCTAACGTTGGAAAGAGCTTTTCCGACGAATCATGGAATTGAGCTGAATCAGCTCAAAGCTAGAGACTTGTTAAGGCATGGTAGATTCTTGCAGTCTTCTTCAAATGGTGTTGTTGATTTCGCTGTTCATGGTACCTTTGATCCATTTCAAGTCGGGCTCTATTTTACAAAGGTGCAGTTAGGTACTCCTCCAGTTGAATTTTATGTGCAAATTGACACTGGAAGTGATGTTCTCTGGGTCAGTTGTAGCTCTTGCAACGGTTGTCCACAGACAAGTGGGTTGCAGATTGAGCTCAATTTTTTCGACCCTAGGAGTTCATCAACATCCTCGTTAATCTCTTGTTCTGACAAAAGGTGCAACAGTGGAATACAATCATCAGACGCAACCTGTTCCAGTCAGACCAACCAGTGCTCTTACACTTTCCAGTATGGAGATGGTAGCGGAACCTCAGGCTATTACGTGTCTGATACAATGCATTTGGATACTATTTTTGAGGGATCCGTGTCTACAAATTCTTCAGCTCCTGTAGTTTTTGGTTGTAGTAACCAGCAGAGTGGAGACTTGACAAAATCTGACAGAGCAGTTGATGGTATATTTGGATTTGGGCAACAGCAAATGTCTGTGATCTCACAGCTCTCATCACAAGGGATTGCTTCTGGAGTATTCTCTCATTGTCTTAGAGGAGATAGCTCTGGTGGAGGAATTCTGGTTCTTGGTGAGATTGTGGAGCCTAACATAGTTTATACTCCACTTGTTCCATCACAGCCTCATTACAACTTAAACCTACAGAGCATATCTGTCAACGGCCAGGCACTTCAAGTCGATCCATCAGTTTTTGCAACATCAAGCAACAGAGGCACCATTGTTGATTCTGGAACAACTTTAGCATACCTTGCAGAAGAGGCTTATGATCCCTTTGTCAATGCGATTACAGCTACAATACCACAATCTGTACGTACGGTTGTTTCCAGGGGAAATCAATGTTTCTTAATTACCGACAGTGTCACTAACATATTTCCTCAAGTAAGTCTGAACTTTGCTGGTGGTGCATCTATGGTTTTAAGACCACAGGACTACCTTATACAACAGAATTCTATTGGTGGTGCAGCAGTGTGGTGTATTGGCTTTCAGAAAAGCCAGGGTCAAGGAGTAACAATTCTAGGAGATCTCGTATTAAAAGACAAAATTGTTGTTTACGATCTGGTCGGTCAACGCATTGGATGGGCTAACTATGACTGTTCTTTGACAGTTAATGTTTCTGCATCAACTGGCACTGGCAGAAGTGAATACGTGAATGCTGGAGAGATAGGTGGAAGCAATTCTTTACATGATGGACTTGCGTCGTCAAAGACAGGTTTATAG
Protein sequence

>Msa1433080

MAVVFIFTAILLTAATVIVSGSPATLTLERAFPTNHGIELNQLKARDLLRHGRFLQSSSNGVVDFAVHGTFDPFQVGLYFTKVQLGTPPVEFYVQIDTGSDVLWVSCSSCNGCPQTSGLQIELNFFDPRSSSTSSLISCSDKRCNSGIQSSDATCSSQTNQCSYTFQYGDGSGTSGYYVSDTMHLDTIFEGSVSTNSSAPVVFGCSNQQSGDLTKSDRAVDGIFGFGQQQMSVISQLSSQGIASGVFSHCLRGDSSGGGILVLGEIVEPNIVYTPLVPSQPHYNLNLQSISVNGQALQVDPSVFATSSNRGTIVDSGTTLAYLAEEAYDPFVNAITATIPQSVRTVVSRGNQCFLITDSVTNIFPQVSLNFAGGASMVLRPQDYLIQQNSIGGAAVWCIGFQKSQGQGVTILGDLVLKDKIVVYDLVGQRIGWANYDCSLTVNVSASTGTGRSEYVNAGEIGGSNSLHDGLASSKTGL*