AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1435660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1435660 MtrunA17_Chr3g0137821 97.939 825 14 1 1 825 1 822 0.0 1687
Msa1435660 MtrunA17_Chr3g0137791 70.167 838 225 12 1 825 1 826 0.0 1197
Msa1435660 MtrunA17_Chr4g0003411 64.269 834 263 12 1 825 1 808 0.0 1083
Msa1435660 MtrunA17_Chr2g0333021 62.936 831 273 11 4 825 5 809 0.0 1038
Msa1435660 MtrunA17_Chr5g0398161 44.686 828 421 16 4 814 37 844 0.0 684
Msa1435660 MtrunA17_Chr7g0246481 44.578 830 418 18 4 814 298 1104 0.0 683
Msa1435660 MtrunA17_Chr8g0380631 44.983 867 405 23 2 825 12 849 0.0 679
Msa1435660 MtrunA17_Chr3g0143471 43.977 880 408 24 5 825 13 866 0.0 678
Msa1435660 MtrunA17_Chr5g0398171 43.824 842 402 19 4 814 37 838 0.0 672
Msa1435660 MtrunA17_Chr5g0406161 42.321 879 427 18 5 825 16 872 0.0 668
Msa1435660 MtrunA17_Chr8g0350951 43.473 835 425 17 4 814 228 1039 0.0 666
Msa1435660 MtrunA17_Chr2g0330431 43.406 781 380 18 53 825 30 756 0.0 621
Msa1435660 MtrunA17_Chr8g0341931 41.276 831 399 21 2 825 5 753 0.0 585
Msa1435660 MtrunA17_Chr1g0192401 32.500 240 123 10 510 740 733 942 2.74e-21 100
Msa1435660 MtrunA17_Chr1g0192401 30.544 239 123 10 211 435 350 559 1.48e-19 94.4
Msa1435660 MtrunA17_Chr2g0295411 33.333 201 102 5 510 705 783 956 2.87e-18 90.1
Msa1435660 MtrunA17_Chr2g0295411 30.453 243 119 11 211 435 376 586 4.97e-18 89.4
Msa1435660 MtrunA17_Chr5g0406151 51.351 74 30 2 326 399 12 79 6.57e-18 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1435660 AT3G15730.1 62.440 828 285 12 3 825 4 810 0.0 1058
Msa1435660 AT1G52570.1 61.911 827 289 12 4 825 5 810 0.0 1038
Msa1435660 AT1G52570.3 61.911 827 289 12 4 825 5 810 0.0 1038
Msa1435660 AT1G52570.2 61.911 827 289 12 4 825 5 810 0.0 1038
Msa1435660 AT5G25370.1 60.434 829 307 14 4 825 6 820 0.0 1001
Msa1435660 AT5G25370.3 60.434 829 307 14 4 825 6 820 0.0 1001
Msa1435660 AT5G25370.2 60.434 829 307 14 4 825 6 820 0.0 1001
Msa1435660 AT5G25370.4 62.716 751 264 12 81 825 5 745 0.0 952
Msa1435660 AT4G11830.2 44.153 838 423 20 1 814 30 846 0.0 685
Msa1435660 AT2G42010.1 44.270 829 419 17 4 814 270 1073 0.0 685
Msa1435660 AT4G11830.1 45.817 777 383 16 59 814 55 814 0.0 684
Msa1435660 AT2G42010.2 43.243 851 421 17 4 814 270 1098 0.0 679
Msa1435660 AT4G11840.1 43.705 842 417 20 3 814 42 856 0.0 672
Msa1435660 AT4G00240.1 43.938 833 416 19 4 814 114 917 0.0 670
Msa1435660 AT4G11850.1 44.019 836 419 19 3 814 38 848 0.0 667
Msa1435660 AT4G35790.2 43.886 875 410 19 4 825 11 857 0.0 667
Msa1435660 AT4G00240.2 45.220 774 387 15 59 814 128 882 0.0 665
Msa1435660 AT4G00240.3 45.220 774 387 15 59 814 11 765 0.0 665
Msa1435660 AT4G35790.1 43.567 886 408 20 4 825 11 868 0.0 661
Msa1435660 AT4G11840.2 43.214 840 394 19 3 814 42 826 0.0 654
Msa1435660 AT1G55180.2 40.875 800 398 23 39 825 3 740 0.0 580
Msa1435660 AT1G55180.1 41.058 794 393 23 45 825 31 762 0.0 579
Msa1435660 AT4G35790.3 42.857 693 323 15 4 643 11 683 1.47e-168 504
Msa1435660 AT4G11830.4 42.697 623 325 15 1 609 30 634 2.05e-160 481
Msa1435660 AT4G11830.3 42.904 606 314 15 1 592 30 617 2.22e-156 471
Msa1435660 AT3G05630.1 34.413 247 125 9 512 750 714 931 2.04e-24 110
Msa1435660 AT3G05630.1 32.218 239 119 10 211 435 363 572 2.66e-19 94.0
Msa1435660 AT3G16785.4 33.182 220 101 9 231 435 389 577 6.04e-20 96.3
Msa1435660 AT3G16785.4 36.453 203 95 7 509 705 756 930 1.05e-19 95.5
Msa1435660 AT3G16785.6 33.182 220 101 9 231 435 389 577 6.41e-20 95.9
Msa1435660 AT3G16785.6 36.453 203 95 7 509 705 756 930 9.76e-20 95.5
Msa1435660 AT3G16785.5 33.182 220 101 9 231 435 389 577 6.41e-20 95.9
Msa1435660 AT3G16785.5 36.453 203 95 7 509 705 756 930 9.76e-20 95.5
Msa1435660 AT3G16785.1 33.182 220 101 9 231 435 389 577 7.01e-20 95.9
Msa1435660 AT3G16785.1 36.453 203 95 7 509 705 756 930 9.96e-20 95.5
Msa1435660 AT3G16785.3 33.182 220 101 9 231 435 389 577 7.38e-20 95.9
Msa1435660 AT3G16785.3 36.453 203 95 7 509 705 756 930 9.54e-20 95.5
Msa1435660 AT3G16785.2 33.182 220 101 9 231 435 389 577 7.38e-20 95.9
Msa1435660 AT3G16785.2 36.453 203 95 7 509 705 756 930 9.54e-20 95.5

Find 203 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACGCATTCCACTCTTTC+AGG 0.164231 tig0027765:+28458 None:intergenic
CAAAGGTCTCATTCCATTTC+GGG 0.167631 tig0027765:+30819 None:intergenic
CTACTTTCTGTTGGCTTGTT+TGG 0.177927 tig0027765:+30305 None:intergenic
GATACAATGGCTTTGATTCT+TGG 0.200605 tig0027765:-28287 Msa1435660:CDS
ATAGAAGCACCTGGGAAATT+TGG 0.213465 tig0027765:+29876 None:intergenic
CCAAATGAAGTTATTGAATT+AGG 0.215359 tig0027765:-29585 Msa1435660:CDS
TTCCACCATCCATTGATCTT+TGG 0.219999 tig0027765:+28630 None:intergenic
GTTGCAAGCTGGATGTAATT+TGG 0.224934 tig0027765:-31406 Msa1435660:CDS
CTAGGACTAGGTGGTAAGTA+TGG 0.233733 tig0027765:+28241 None:intergenic
CAACCTATCAACTTCACTTA+TGG 0.246044 tig0027765:+31425 None:intergenic
TACTTTCTGTTGGCTTGTTT+GGG 0.247108 tig0027765:+30306 None:intergenic
GGAAGTGTGACATCACTTTC+AGG 0.249099 tig0027765:-28326 Msa1435660:CDS
TAAAATACTCTGCAGTTTCT+TGG 0.255279 tig0027765:+30159 None:intergenic
TCAAAGGTCTCATTCCATTT+CGG 0.277608 tig0027765:+30818 None:intergenic
GGGTTGATTCCCTATCATTC+TGG 0.280304 tig0027765:+30847 None:intergenic
TTAATGTTCTTGTGCTTGTT+TGG 0.292615 tig0027765:-30238 Msa1435660:CDS
ACAAACGGGAACTATTTCTT+TGG 0.299627 tig0027765:+31593 None:intergenic
TCAACCCATCTGTCAATTAT+AGG 0.308791 tig0027765:+30680 None:intergenic
AAAGTCTTTATTTACATAAC+TGG 0.310682 tig0027765:-30365 Msa1435660:CDS
ATACAGAAATAACCTTGATT+AGG 0.319930 tig0027765:-30331 Msa1435660:CDS
ATTCTATCAAGCTCTTGAAT+TGG 0.328163 tig0027765:+29717 None:intergenic
CATCAACAACTATAGTCTTT+TGG 0.330540 tig0027765:+30039 None:intergenic
AAGAACATTAACACCTTCAT+TGG 0.337331 tig0027765:+30250 None:intergenic
CCTAATTCAATAACTTCATT+TGG 0.339645 tig0027765:+29585 None:intergenic
TACAGAAATAACCTTGATTA+GGG 0.344363 tig0027765:-30330 Msa1435660:CDS
CTCATTCCATTTCGGGTTTG+AGG 0.344995 tig0027765:+30826 None:intergenic
AGAATCAAAGCCATTGTATC+AGG 0.349603 tig0027765:+28290 None:intergenic
CTTCAACAGGATACCGTAAA+AGG 0.350159 tig0027765:+28360 None:intergenic
GGAACAAAATATTATGTTCC+TGG 0.351442 tig0027765:-30428 Msa1435660:CDS
CGAACAGTTATCAGTTTCAT+AGG 0.359772 tig0027765:-29990 Msa1435660:CDS
GATATTAAAGTTGAGGATAT+TGG 0.365230 tig0027765:-29426 Msa1435660:CDS
CATCCATGGATACCAATATC+TGG 0.368621 tig0027765:-30449 Msa1435660:CDS
TTCTATCAAGCTCTTGAATT+GGG 0.378843 tig0027765:+29718 None:intergenic
CCAATTGGTGCAACTCTAAT+CGG 0.382314 tig0027765:-30740 Msa1435660:CDS
AGACCACAAGGTCAAGTATA+TGG 0.383000 tig0027765:-28539 Msa1435660:CDS
GTTGGACGAACCAGAATGAT+AGG 0.386126 tig0027765:-30857 Msa1435660:CDS
CATCTTACCTATCATCATCT+TGG 0.386141 tig0027765:+29044 None:intergenic
ATATCTTCCCAACATCTTCC+AGG 0.387093 tig0027765:+30410 None:intergenic
ACCTGAGAGTGCATCAGTTC+AGG 0.390624 tig0027765:-29295 Msa1435660:CDS
CATGATTTCATCAAAGTGTA+AGG 0.391165 tig0027765:+28153 None:intergenic
CTGTCAGGCTCTGGTTGCTC+TGG 0.392079 tig0027765:+29117 None:intergenic
CCATCTTCACCCACTTCAAC+AGG 0.395226 tig0027765:+28347 None:intergenic
CCGATTAGAGTTGCACCAAT+TGG 0.395417 tig0027765:+30740 None:intergenic
TAATGTTCTTGTGCTTGTTT+GGG 0.399896 tig0027765:-30237 Msa1435660:CDS
ATTGAAAATCAGTATTTCCT+AGG 0.407400 tig0027765:-29474 Msa1435660:CDS
CAGTTCAGGCAATATTAGAT+TGG 0.408091 tig0027765:-29281 Msa1435660:CDS
ACCGTGTTGCTGAGAGGAAT+TGG 0.410354 tig0027765:-28427 Msa1435660:CDS
ATCAACCCTCAAACCCGAAA+TGG 0.413276 tig0027765:-30832 Msa1435660:CDS
CAGAAGAAGATGGCGAAACT+TGG 0.417133 tig0027765:-29665 Msa1435660:CDS
CAAAAGACTATAGTTGTTGA+TGG 0.423538 tig0027765:-30038 Msa1435660:CDS
CTTGTTTGGGTCCCTAATCA+AGG 0.426022 tig0027765:+30319 None:intergenic
GCATTATGGTATGAACATAT+TGG 0.427241 tig0027765:-28506 Msa1435660:CDS
CAAGCTCTTGAATTGGGAAC+AGG 0.427300 tig0027765:+29724 None:intergenic
AAAGACTTTAGCATTCATAA+TGG 0.428519 tig0027765:+30379 None:intergenic
GGAAATAATGGCTGAACAAA+CGG 0.432945 tig0027765:+31578 None:intergenic
ATGGCTTCAGGAGAGCATTA+TGG 0.435411 tig0027765:-28520 Msa1435660:CDS
GATATTCACTGCAAGCTAGA+AGG 0.435535 tig0027765:-29822 Msa1435660:CDS
GTCAAAGATGATAATCCAAT+TGG 0.435782 tig0027765:-30755 Msa1435660:CDS
TCATTCCATTTCGGGTTTGA+GGG 0.436659 tig0027765:+30827 None:intergenic
GTTCATTCCAAGATGATGAT+AGG 0.436783 tig0027765:-29051 Msa1435660:intron
TAACCATAAGTGAAGTTGAT+AGG 0.438118 tig0027765:-31428 Msa1435660:CDS
ATCTTCAATGACAACTCCTT+TGG 0.440344 tig0027765:+29387 None:intergenic
ATCCATGGATGAACACTGTC+AGG 0.442733 tig0027765:+30461 None:intergenic
GAATTGGGAACAGGAATTGT+TGG 0.442982 tig0027765:+29733 None:intergenic
TCCTAGGGAGTTCCTATGGT+TGG 0.446572 tig0027765:-29458 Msa1435660:CDS
GCTGCTACATGGGACACTTA+AGG 0.452370 tig0027765:-31451 Msa1435660:CDS
ACAAGATACAACCCTTGACT+TGG 0.452543 tig0027765:+31040 None:intergenic
TTTCTTGAGTATTTCTCCTA+GGG 0.452951 tig0027765:+30277 None:intergenic
GCACTCTCAGGTATACCTTC+TGG 0.455321 tig0027765:+29306 None:intergenic
TTGTGGTCTTTGTGAGCCAT+TGG 0.456429 tig0027765:+28553 None:intergenic
CCAGGTGCTTCTATTAAGAA+AGG 0.457313 tig0027765:-29867 Msa1435660:CDS
GGACGACAGAACCTCTGTTC+CGG 0.460427 tig0027765:-30216 Msa1435660:CDS
AAGGTCAAGTATATGGCTTC+AGG 0.460768 tig0027765:-28532 Msa1435660:CDS
CAGTTGTTTAGATCCATTGA+TGG 0.464754 tig0027765:-29636 Msa1435660:CDS
GTTCCAGATATTGGTATCCA+TGG 0.465135 tig0027765:+30446 None:intergenic
TAACTTCATTTGGTGCTTGA+GGG 0.468911 tig0027765:+29595 None:intergenic
TTATGTTCCTGGAAGATGTT+GGG 0.469431 tig0027765:-30417 Msa1435660:CDS
AAAATGCCTCAGCTGCTACA+TGG 0.472016 tig0027765:-31462 Msa1435660:CDS
ATAACTTCATTTGGTGCTTG+AGG 0.473405 tig0027765:+29594 None:intergenic
ATTGTTAGCAAGATTGAAGC+TGG 0.474341 tig0027765:-29366 Msa1435660:CDS
GAAATAATGGCTGAACAAAC+GGG 0.474517 tig0027765:+31579 None:intergenic
AGAAACAGCACCACCATCAA+TGG 0.474765 tig0027765:+29623 None:intergenic
CCTGTTGAAGTGGGTGAAGA+TGG 0.476067 tig0027765:-28347 Msa1435660:CDS
AAAAGGGAGAGTAAGAAAGA+TGG 0.477240 tig0027765:-29153 Msa1435660:CDS
GCCTGAACTGATGCACTCTC+AGG 0.477938 tig0027765:+29294 None:intergenic
TATTTCCTAGGGAGTTCCTA+TGG 0.478923 tig0027765:-29462 Msa1435660:CDS
TTTGCGATCCTTCAACATGA+TGG 0.480827 tig0027765:+30015 None:intergenic
TGATAATCACTGTCAGGCTC+TGG 0.481330 tig0027765:+29108 None:intergenic
ACTTGCTCGTTCATACACTA+TGG 0.481773 tig0027765:-28100 Msa1435660:three_prime_UTR
TTATGATAACAACCTTGTCT+TGG 0.481826 tig0027765:+28195 None:intergenic
TCTTTGTGAGCCATTGGATG+TGG 0.483037 tig0027765:+28559 None:intergenic
ATTGAATTAGGCCTTGTTAG+TGG 0.484468 tig0027765:-29573 Msa1435660:CDS
GTTGATGAAAATCATCATGA+TGG 0.485239 tig0027765:+29898 None:intergenic
GATATAGATCATAGCATAGC+AGG 0.485628 tig0027765:-31511 Msa1435660:five_prime_UTR
GAAGATCATCGTCCTGTACC+AGG 0.485983 tig0027765:-30644 Msa1435660:CDS
GATTTAGATAAAGCAAGGGT+TGG 0.486390 tig0027765:-30875 Msa1435660:CDS
CAATATTAGATTGGCAAAGA+AGG 0.486949 tig0027765:-29272 Msa1435660:CDS
TCTTTATTTACATAACTGGT+TGG 0.487503 tig0027765:-30361 Msa1435660:CDS
TCAGGCTCTGGTTGCTCTGG+AGG 0.487804 tig0027765:+29120 None:intergenic
CACTTGTGATGAAGATTGTT+GGG 0.493040 tig0027765:-30919 Msa1435660:intron
TACAACACATCCCATGCAAT+AGG 0.493852 tig0027765:+29798 None:intergenic
AGAGGTGGGAAAAGCAAGTC+GGG 0.494783 tig0027765:-29764 Msa1435660:CDS
AGGGTGATACTACTTTCTGT+TGG 0.495579 tig0027765:+30296 None:intergenic
CCACTTGTGATGAAGATTGT+TGG 0.497598 tig0027765:-30920 Msa1435660:intron
TCTTCGTATAGCATTTATGT+AGG 0.504718 tig0027765:+29515 None:intergenic
GCTCTATGATAATCACTGTC+AGG 0.504896 tig0027765:+29102 None:intergenic
ATATCATGCCACGGTTCTCT+AGG 0.506247 tig0027765:+29840 None:intergenic
AGGTGGTAAGTATGGGAGGA+AGG 0.511866 tig0027765:+28249 None:intergenic
CCTTTCTTAATAGAAGCACC+TGG 0.512394 tig0027765:+29867 None:intergenic
TTTACGGTATCCTGTTGAAG+TGG 0.514342 tig0027765:-28357 Msa1435660:CDS
AAACCGTCTTTCTTAAAAGC+CGG 0.515049 tig0027765:+30197 None:intergenic
TTCAACTGATATTAAAGTTG+AGG 0.520279 tig0027765:-29433 Msa1435660:CDS
TTGAAAATCAGTATTTCCTA+GGG 0.521701 tig0027765:-29473 Msa1435660:CDS
TAATTTGGATTTCTGTCGAA+AGG 0.523952 tig0027765:-31391 Msa1435660:intron
AAATGCCTCAGCTGCTACAT+GGG 0.524555 tig0027765:-31461 Msa1435660:CDS
AGAGAGGTTTGTTGTGTATG+TGG 0.525642 tig0027765:-29343 Msa1435660:CDS
AAGCACTGTGCTAGCTAGCT+AGG 0.526978 tig0027765:+28223 None:intergenic
GGGGTCGGGAGAGGAAATAA+TGG 0.528859 tig0027765:+31566 None:intergenic
TTTAAGAAAGACGGTTTGAT+GGG 0.532749 tig0027765:-30191 Msa1435660:CDS
TTGAGGATAGAAAATGTCTA+TGG 0.533093 tig0027765:-30580 Msa1435660:CDS
CTAGCTAGCACAGTGCTTGA+CGG 0.534749 tig0027765:-28218 Msa1435660:three_prime_UTR
AAACATAAACCAAAGATCAA+TGG 0.535452 tig0027765:-28639 Msa1435660:CDS
TGATGAAATCATGTGGTACA+AGG 0.537673 tig0027765:-28143 Msa1435660:three_prime_UTR
AGAAAATGTCTATGGTCTCA+AGG 0.538595 tig0027765:-30572 Msa1435660:CDS
TCTGATAACATTGCATCAGA+AGG 0.542244 tig0027765:+29684 None:intergenic
AGCTAGAAGGACCTATTGCA+TGG 0.543044 tig0027765:-29809 Msa1435660:CDS
ATTATGTTCCTGGAAGATGT+TGG 0.543312 tig0027765:-30418 Msa1435660:CDS
TTTCATCAACCAAATTTCCC+AGG 0.543862 tig0027765:-29885 Msa1435660:CDS
GGTATACCTTCTGGCCACAT+TGG 0.545537 tig0027765:+29315 None:intergenic
AAGGACCTATAATTGACAGA+TGG 0.549514 tig0027765:-30685 Msa1435660:CDS
TCAAGGGTTGTATCTTGTGC+CGG 0.551228 tig0027765:-31035 Msa1435660:CDS
GAAGTGTGACATCACTTTCA+GGG 0.551309 tig0027765:-28325 Msa1435660:CDS
TTCGATCAGAGACAAGGTTG+TGG 0.553785 tig0027765:-30512 Msa1435660:CDS
GTTGATGGCCATCATGTTGA+AGG 0.554676 tig0027765:-30023 Msa1435660:CDS
GACTAGGTGGTAAGTATGGG+AGG 0.555923 tig0027765:+28245 None:intergenic
TTGTATCTTGTGCCGGCCTG+AGG 0.558222 tig0027765:-31028 Msa1435660:intron
AGAGACACAGAGATTGCAAT+GGG 0.562687 tig0027765:-28608 Msa1435660:CDS
ATAACATTGCATCAGAAGGA+TGG 0.563136 tig0027765:+29688 None:intergenic
CAGTTGATTTAGATAAAGCA+AGG 0.564307 tig0027765:-30880 Msa1435660:CDS
AGGACCTATAATTGACAGAT+GGG 0.565090 tig0027765:-30684 Msa1435660:CDS
GATGATGAATACATAGTGAT+TGG 0.566916 tig0027765:-28668 Msa1435660:CDS
TTACGGTATCCTGTTGAAGT+GGG 0.569394 tig0027765:-28356 Msa1435660:CDS
TGTGCTAGCTAGCTAGGACT+AGG 0.570468 tig0027765:+28229 None:intergenic
GCAATTCTCTAGTGTCATTG+AGG 0.571602 tig0027765:-30597 Msa1435660:CDS
TCCCTGACAGTGTTCATCCA+TGG 0.573348 tig0027765:-30463 Msa1435660:CDS
CAAAATGTAGGGGTCGGGAG+AGG 0.575553 tig0027765:+31557 None:intergenic
GAGATTTCTAGCCCAAGTCA+AGG 0.578002 tig0027765:-31052 Msa1435660:CDS
TAGGACTAGGTGGTAAGTAT+GGG 0.578728 tig0027765:+28242 None:intergenic
CTCAGTATACATCATCTCCA+TGG 0.584166 tig0027765:+29248 None:intergenic
TGTCAGGGACATGAGCGTCT+TGG 0.584673 tig0027765:+30477 None:intergenic
CAAGATACAACCCTTGACTT+GGG 0.589678 tig0027765:+31041 None:intergenic
CATCTAGCATCCACATCCAA+TGG 0.592837 tig0027765:-28569 Msa1435660:CDS
TTATGTCCGCGTAACCCTGA+TGG 0.596322 tig0027765:-30113 Msa1435660:CDS
GCCAACCATAGGAACTCCCT+AGG 0.597582 tig0027765:+29457 None:intergenic
GCAAGATTGAAGCTGGAGAG+AGG 0.597835 tig0027765:-29359 Msa1435660:CDS
TCCAATTCCTCTCAGCAACA+CGG 0.598333 tig0027765:+28426 None:intergenic
GAAGTTGATAGGTTGCAAGC+TGG 0.598778 tig0027765:-31417 Msa1435660:CDS
ATGAGCACAATAGATTTCAA+AGG 0.601968 tig0027765:+30802 None:intergenic
TGCATTGCATCTCATACCAA+AGG 0.606137 tig0027765:-29403 Msa1435660:CDS
AGATTTCTAGCCCAAGTCAA+GGG 0.608267 tig0027765:-31051 Msa1435660:CDS
ATATAGATCATAGCATAGCA+GGG 0.609293 tig0027765:-31510 Msa1435660:five_prime_UTR
TTGTTTAGATCCATTGATGG+TGG 0.610089 tig0027765:-29633 Msa1435660:CDS
ATCAGTTGAATGCCAACCAT+AGG 0.610416 tig0027765:+29446 None:intergenic
TTAAATTGTGTACCTCAGGC+CGG 0.610436 tig0027765:+31016 None:intergenic
AACCAAAGATCAATGGATGG+TGG 0.611885 tig0027765:-28632 Msa1435660:CDS
CAGAGGTGGGAAAAGCAAGT+CGG 0.616576 tig0027765:-29765 Msa1435660:CDS
TTTCCACCATCAGGGTTACG+CGG 0.618021 tig0027765:+30107 None:intergenic
AGTTGATTTAGATAAAGCAA+GGG 0.621560 tig0027765:-30879 Msa1435660:CDS
TACACTTTGATGAAATCATG+TGG 0.621582 tig0027765:-28150 Msa1435660:three_prime_UTR
TTGGACGAACCAGAATGATA+GGG 0.623042 tig0027765:-30856 Msa1435660:CDS
ACAGAAAGTAGTATCACCCT+AGG 0.623457 tig0027765:-30293 Msa1435660:CDS
AGTGTCCCATGTAGCAGCTG+AGG 0.627470 tig0027765:+31456 None:intergenic
GGTGGTATTGATCTGTGTGA+TGG 0.628015 tig0027765:-29969 Msa1435660:CDS
TAAAGGGAACAACACACAAG+GGG 0.628918 tig0027765:-31077 Msa1435660:intron
AGTGCTTGACGGCCAAGACA+AGG 0.628973 tig0027765:-28207 Msa1435660:three_prime_UTR
GTGTTCAAAGTTGAAAACAA+AGG 0.629611 tig0027765:+29924 None:intergenic
GCTAGAAGGACCTATTGCAT+GGG 0.631026 tig0027765:-29808 Msa1435660:CDS
TGTCCGCGTAACCCTGATGG+TGG 0.631372 tig0027765:-30110 Msa1435660:CDS
ACAGTTATCAGTTTCATAGG+TGG 0.633175 tig0027765:-29987 Msa1435660:CDS
ATAAACCAAAGATCAATGGA+TGG 0.633380 tig0027765:-28635 Msa1435660:CDS
CCTTCCGAGAAAATTCTCAA+AGG 0.639521 tig0027765:-30704 Msa1435660:CDS
AAGCCATATACTTGACCTTG+TGG 0.641516 tig0027765:+28536 None:intergenic
TTTCTTAAAAGCCGGAACAG+AGG 0.644428 tig0027765:+30205 None:intergenic
CTTTCTTAATAGAAGCACCT+GGG 0.646815 tig0027765:+29868 None:intergenic
ATAGTGTATGAACGAGCAAG+TGG 0.648306 tig0027765:+28102 None:intergenic
GGTGCTTCTATTAAGAAAGG+TGG 0.653659 tig0027765:-29864 Msa1435660:CDS
GTGATTCCAATGTGGCCAGA+AGG 0.653982 tig0027765:-29321 Msa1435660:CDS
GCAATGTTATCAGAAGAAGA+TGG 0.655613 tig0027765:-29675 Msa1435660:CDS
AAGAGACACAGAGATTGCAA+TGG 0.659014 tig0027765:-28609 Msa1435660:CDS
TTGCAGTGAATATCATGCCA+CGG 0.660624 tig0027765:+29831 None:intergenic
AATAAAGGGAACAACACACA+AGG 0.665452 tig0027765:-31079 Msa1435660:intron
AGATTGGCAAAGAAGGACCA+TGG 0.665799 tig0027765:-29265 Msa1435660:CDS
GCTAGCTAGCTAGGACTAGG+TGG 0.669608 tig0027765:+28232 None:intergenic
TGATGGAAGATATGATACAA+TGG 0.676865 tig0027765:-29952 Msa1435660:CDS
GATGTGGATGCTAGATGATG+AGG 0.676901 tig0027765:+28575 None:intergenic
TAAAATGTCAAATAATCACG+AGG 0.677762 tig0027765:+29186 None:intergenic
AAAATGTCAAATAATCACGA+GGG 0.681608 tig0027765:+29187 None:intergenic
TCCATGGATGAACACTGTCA+GGG 0.688183 tig0027765:+30462 None:intergenic
ATAAAGGGAACAACACACAA+GGG 0.688691 tig0027765:-31078 Msa1435660:intron
AATGGCTCACAAAGACCACA+AGG 0.712737 tig0027765:-28551 Msa1435660:CDS
TGTATGTGGTGATTCCAATG+TGG 0.713699 tig0027765:-29329 Msa1435660:CDS
GATCATCGTCCTGTACCAGG+CGG 0.755483 tig0027765:-30641 Msa1435660:CDS
AAGGTGGTCCTAGAGAACCG+TGG 0.794041 tig0027765:-29848 Msa1435660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATTTTTTACAAAATGTA+GGG + tig0027765:28108-28127 None:intergenic 15.0%
!!! TTCAATTTTTTACAAAATGT+AGG + tig0027765:28109-28128 None:intergenic 15.0%
!! TTGAAGTTTAAATTCATAAA+TGG - tig0027765:28345-28364 Msa1435660:CDS 15.0%
!! CATATAAGCTATTATATTAT+AGG + tig0027765:30642-30661 None:intergenic 15.0%
!! AAAAGATTTATTTGAATGTT+TGG + tig0027765:30679-30698 None:intergenic 15.0%
!!! ACATTCAAATAAATCTTTTT+AGG - tig0027765:30680-30699 Msa1435660:CDS 15.0%
!! AAACCTAGAAAATATATATT+TGG - tig0027765:30874-30893 Msa1435660:CDS 15.0%
!!! CAATTTTTTACAAAATGTAG+GGG + tig0027765:28107-28126 None:intergenic 20.0%
!! CACTTTAATTATTAAAACAC+TGG + tig0027765:28306-28325 None:intergenic 20.0%
!! AAATGGCAAAATATATTATG+CGG - tig0027765:28362-28381 Msa1435660:CDS 20.0%
!! AATTTGTGTATTCAATGAAT+TGG - tig0027765:28453-28472 Msa1435660:CDS 20.0%
!!! TAATATTTTGTTCCAGATAT+TGG + tig0027765:29217-29236 None:intergenic 20.0%
!!! AAAGTCTTTATTTACATAAC+TGG - tig0027765:29286-29305 Msa1435660:CDS 20.0%
!!! AGAGTATTTTAAAAACACAA+AGG - tig0027765:29504-29523 Msa1435660:CDS 20.0%
!!! TTCAACATTCTTCTTTTTTA+TGG + tig0027765:30439-30458 None:intergenic 20.0%
!!! TATTTGACATTTTATTGTCT+TGG - tig0027765:30474-30493 Msa1435660:CDS 20.0%
!!! ATTTTATTGTCTTGGTAAAA+GGG - tig0027765:30482-30501 Msa1435660:CDS 20.0%
!!! TTCAAATAAATCTTTTTAGG+TGG - tig0027765:30683-30702 Msa1435660:CDS 20.0%
!! AATGAAAAATCATGATAGTT+AGG + tig0027765:30839-30858 None:intergenic 20.0%
!!! ATTTTCTAGGTTTTGAATAT+AGG + tig0027765:30867-30886 None:intergenic 20.0%
!!! ATGCCAAATATATATTTTCT+AGG + tig0027765:30880-30899 None:intergenic 20.0%
!! ATAAAGTAAACAATCAATGT+TGG + tig0027765:30948-30967 None:intergenic 20.0%
! CAAAATATATTATGCGGTAT+TGG - tig0027765:28368-28387 Msa1435660:CDS 25.0%
!!! AAAGTCTTTAAAAAGTGTCA+CGG + tig0027765:28414-28433 None:intergenic 25.0%
! AAGAAAAAAATATAGTGCCA+CGG + tig0027765:28536-28555 None:intergenic 25.0%
!! TTTTCTTCTGTCAAATATTG+TGG - tig0027765:28549-28568 Msa1435660:CDS 25.0%
! TGTCAAATATTGTGGAATAA+AGG - tig0027765:28557-28576 Msa1435660:CDS 25.0%
! GTCAAATATTGTGGAATAAA+GGG - tig0027765:28558-28577 Msa1435660:CDS 25.0%
! TATACAAATGGTAGAAAGTT+TGG + tig0027765:28661-28680 None:intergenic 25.0%
!! ACATGAAAAGCATATACAAA+TGG + tig0027765:28673-28692 None:intergenic 25.0%
!! AGTTGATTTAGATAAAGCAA+GGG - tig0027765:28772-28791 Msa1435660:intron 25.0%
! AAAGACTTTAGCATTCATAA+TGG + tig0027765:29275-29294 None:intergenic 25.0%
!! TCTTTATTTACATAACTGGT+TGG - tig0027765:29290-29309 Msa1435660:CDS 25.0%
! ATACAGAAATAACCTTGATT+AGG - tig0027765:29320-29339 Msa1435660:CDS 25.0%
! TACAGAAATAACCTTGATTA+GGG - tig0027765:29321-29340 Msa1435660:CDS 25.0%
! CCTAATTCAATAACTTCATT+TGG + tig0027765:30069-30088 None:intergenic 25.0%
! CCAAATGAAGTTATTGAATT+AGG - tig0027765:30066-30085 Msa1435660:CDS 25.0%
! ATTGAAAATCAGTATTTCCT+AGG - tig0027765:30177-30196 Msa1435660:CDS 25.0%
! TTGAAAATCAGTATTTCCTA+GGG - tig0027765:30178-30197 Msa1435660:CDS 25.0%
! TTCAACTGATATTAAAGTTG+AGG - tig0027765:30218-30237 Msa1435660:CDS 25.0%
! GATATTAAAGTTGAGGATAT+TGG - tig0027765:30225-30244 Msa1435660:CDS 25.0%
! AAAATGTCAAATAATCACGA+GGG + tig0027765:30467-30486 None:intergenic 25.0%
! TAAAATGTCAAATAATCACG+AGG + tig0027765:30468-30487 None:intergenic 25.0%
!! CATTTTATTGTCTTGGTAAA+AGG - tig0027765:30481-30500 Msa1435660:CDS 25.0%
! AAAAATCATGATAGTTAGGT+AGG + tig0027765:30835-30854 None:intergenic 25.0%
! AAACATAAACCAAAGATCAA+TGG - tig0027765:31012-31031 Msa1435660:intron 25.0%
! AAAAATCTATGAAACAAGTG+AGG + tig0027765:31577-31596 None:intergenic 25.0%
! TTTTTACAAAATGTAGGGGT+CGG + tig0027765:28103-28122 None:intergenic 30.0%
ATATAGATCATAGCATAGCA+GGG - tig0027765:28141-28160 Msa1435660:three_prime_UTR 30.0%
! TAACCATAAGTGAAGTTGAT+AGG - tig0027765:28223-28242 Msa1435660:three_prime_UTR 30.0%
TAATTTGGATTTCTGTCGAA+AGG - tig0027765:28260-28279 Msa1435660:CDS 30.0%
!! AAGTCTTTAAAAAGTGTCAC+GGG + tig0027765:28413-28432 None:intergenic 30.0%
! AGCTCATTTAATTTAATCCG+TGG - tig0027765:28516-28535 Msa1435660:CDS 30.0%
! CAGTTGATTTAGATAAAGCA+AGG - tig0027765:28771-28790 Msa1435660:intron 30.0%
ATGAGCACAATAGATTTCAA+AGG + tig0027765:28852-28871 None:intergenic 30.0%
GTCAAAGATGATAATCCAAT+TGG - tig0027765:28896-28915 Msa1435660:intron 30.0%
TTGAGGATAGAAAATGTCTA+TGG - tig0027765:29071-29090 Msa1435660:CDS 30.0%
GGAACAAAATATTATGTTCC+TGG - tig0027765:29223-29242 Msa1435660:CDS 30.0%
TTTCTTGAGTATTTCTCCTA+GGG + tig0027765:29377-29396 None:intergenic 30.0%
! TTTTCTTGAGTATTTCTCCT+AGG + tig0027765:29378-29397 None:intergenic 30.0%
AAGAACATTAACACCTTCAT+TGG + tig0027765:29404-29423 None:intergenic 30.0%
TTAATGTTCTTGTGCTTGTT+TGG - tig0027765:29413-29432 Msa1435660:CDS 30.0%
TAATGTTCTTGTGCTTGTTT+GGG - tig0027765:29414-29433 Msa1435660:CDS 30.0%
!! TTTTAAGAAAGACGGTTTGA+TGG - tig0027765:29459-29478 Msa1435660:CDS 30.0%
TTTAAGAAAGACGGTTTGAT+GGG - tig0027765:29460-29479 Msa1435660:CDS 30.0%
TAAAATACTCTGCAGTTTCT+TGG + tig0027765:29495-29514 None:intergenic 30.0%
! CATCAACAACTATAGTCTTT+TGG + tig0027765:29615-29634 None:intergenic 30.0%
CAAAAGACTATAGTTGTTGA+TGG - tig0027765:29613-29632 Msa1435660:CDS 30.0%
TGATGGAAGATATGATACAA+TGG - tig0027765:29699-29718 Msa1435660:CDS 30.0%
GTGTTCAAAGTTGAAAACAA+AGG + tig0027765:29730-29749 None:intergenic 30.0%
! GTTGATGAAAATCATCATGA+TGG + tig0027765:29756-29775 None:intergenic 30.0%
TTCTATCAAGCTCTTGAATT+GGG + tig0027765:29936-29955 None:intergenic 30.0%
ATTCTATCAAGCTCTTGAAT+TGG + tig0027765:29937-29956 None:intergenic 30.0%
TCTTCGTATAGCATTTATGT+AGG + tig0027765:30139-30158 None:intergenic 30.0%
CAATATTAGATTGGCAAAGA+AGG - tig0027765:30379-30398 Msa1435660:CDS 30.0%
TGAATGTTTGGACTTTGTAA+AGG + tig0027765:30667-30686 None:intergenic 30.0%
!!! ATCTTTTTAGGTGGTTTCTA+AGG - tig0027765:30692-30711 Msa1435660:CDS 30.0%
GAAACTCTAACAACTATCAA+TGG - tig0027765:30767-30786 Msa1435660:CDS 30.0%
ATTTAGTCCAATCTTCTTAC+GGG + tig0027765:30798-30817 None:intergenic 30.0%
CATTTAGTCCAATCTTCTTA+CGG + tig0027765:30799-30818 None:intergenic 30.0%
!!! ACTTTATTTTGTTCCTGTAG+TGG - tig0027765:30961-30980 Msa1435660:intron 30.0%
GATGATGAATACATAGTGAT+TGG - tig0027765:30983-31002 Msa1435660:intron 30.0%
ATAAACCAAAGATCAATGGA+TGG - tig0027765:31016-31035 Msa1435660:intron 30.0%
GCATTATGGTATGAACATAT+TGG - tig0027765:31145-31164 Msa1435660:intron 30.0%
! GTGTAAATACTCACCTTTTA+CGG - tig0027765:31278-31297 Msa1435660:intron 30.0%
! GCAATATTTTCCTGATACAA+TGG - tig0027765:31351-31370 Msa1435660:intron 30.0%
TTATGATAACAACCTTGTCT+TGG + tig0027765:31459-31478 None:intergenic 30.0%
CATGATTTCATCAAAGTGTA+AGG + tig0027765:31501-31520 None:intergenic 30.0%
TACACTTTGATGAAATCATG+TGG - tig0027765:31501-31520 Msa1435660:five_prime_UTR 30.0%
GAAATAATGGCTGAACAAAC+GGG + tig0027765:28075-28094 None:intergenic 35.0%
GGAAATAATGGCTGAACAAA+CGG + tig0027765:28076-28095 None:intergenic 35.0%
! TTTTACAAAATGTAGGGGTC+GGG + tig0027765:28102-28121 None:intergenic 35.0%
GATATAGATCATAGCATAGC+AGG - tig0027765:28140-28159 Msa1435660:three_prime_UTR 35.0%
CAACCTATCAACTTCACTTA+TGG + tig0027765:28229-28248 None:intergenic 35.0%
AATAAAGGGAACAACACACA+AGG - tig0027765:28572-28591 Msa1435660:CDS 35.0%
ATAAAGGGAACAACACACAA+GGG - tig0027765:28573-28592 Msa1435660:CDS 35.0%
TTGGTTAAATTGTGTACCTC+AGG + tig0027765:28642-28661 None:intergenic 35.0%
CACTTGTGATGAAGATTGTT+GGG - tig0027765:28732-28751 Msa1435660:intron 35.0%
GATTTAGATAAAGCAAGGGT+TGG - tig0027765:28776-28795 Msa1435660:intron 35.0%
TCAAAGGTCTCATTCCATTT+CGG + tig0027765:28836-28855 None:intergenic 35.0%
!! CTTTGAGAATTTTCTCGGAA+GGG + tig0027765:28949-28968 None:intergenic 35.0%
!! AGGTCCTTTGAGAATTTTCT+CGG + tig0027765:28954-28973 None:intergenic 35.0%
AAGGACCTATAATTGACAGA+TGG - tig0027765:28966-28985 Msa1435660:intron 35.0%
AGGACCTATAATTGACAGAT+GGG - tig0027765:28967-28986 Msa1435660:intron 35.0%
TCAACCCATCTGTCAATTAT+AGG + tig0027765:28974-28993 None:intergenic 35.0%
AGAAAATGTCTATGGTCTCA+AGG - tig0027765:29079-29098 Msa1435660:CDS 35.0%
!!! AGGAATACACATGCCATTTT+TGG - tig0027765:29099-29118 Msa1435660:CDS 35.0%
!!! GGAATACACATGCCATTTTT+GGG - tig0027765:29100-29119 Msa1435660:CDS 35.0%
!! ACATTTTTCGATCAGAGACA+AGG - tig0027765:29133-29152 Msa1435660:CDS 35.0%
ATTATGTTCCTGGAAGATGT+TGG - tig0027765:29233-29252 Msa1435660:CDS 35.0%
TTATGTTCCTGGAAGATGTT+GGG - tig0027765:29234-29253 Msa1435660:CDS 35.0%
!! TACTTTCTGTTGGCTTGTTT+GGG + tig0027765:29348-29367 None:intergenic 35.0%
CTCAAGAAAAAAGCCAATGA+AGG - tig0027765:29388-29407 Msa1435660:CDS 35.0%
AAACCGTCTTTCTTAAAAGC+CGG + tig0027765:29457-29476 None:intergenic 35.0%
CGAACAGTTATCAGTTTCAT+AGG - tig0027765:29661-29680 Msa1435660:CDS 35.0%
ACAGTTATCAGTTTCATAGG+TGG - tig0027765:29664-29683 Msa1435660:CDS 35.0%
TTTCATCAACCAAATTTCCC+AGG - tig0027765:29766-29785 Msa1435660:CDS 35.0%
!! CTTTCTTAATAGAAGCACCT+GGG + tig0027765:29786-29805 None:intergenic 35.0%
! TGTTGTACAATTTTGAGCAG+AGG - tig0027765:29869-29888 Msa1435660:CDS 35.0%
ATAACATTGCATCAGAAGGA+TGG + tig0027765:29966-29985 None:intergenic 35.0%
TCTGATAACATTGCATCAGA+AGG + tig0027765:29970-29989 None:intergenic 35.0%
GCAATGTTATCAGAAGAAGA+TGG - tig0027765:29976-29995 Msa1435660:CDS 35.0%
CAGTTGTTTAGATCCATTGA+TGG - tig0027765:30015-30034 Msa1435660:CDS 35.0%
TTGTTTAGATCCATTGATGG+TGG - tig0027765:30018-30037 Msa1435660:CDS 35.0%
!! TAACTTCATTTGGTGCTTGA+GGG + tig0027765:30059-30078 None:intergenic 35.0%
!! ATAACTTCATTTGGTGCTTG+AGG + tig0027765:30060-30079 None:intergenic 35.0%
!! ATTGAATTAGGCCTTGTTAG+TGG - tig0027765:30078-30097 Msa1435660:CDS 35.0%
! TGTTGTCTTTTCCACTAACA+AGG + tig0027765:30092-30111 None:intergenic 35.0%
ATCTTCAATGACAACTCCTT+TGG + tig0027765:30267-30286 None:intergenic 35.0%
ATTGTTAGCAAGATTGAAGC+TGG - tig0027765:30285-30304 Msa1435660:CDS 35.0%
CAGTTCAGGCAATATTAGAT+TGG - tig0027765:30370-30389 Msa1435660:CDS 35.0%
AAAAGGGAGAGTAAGAAAGA+TGG - tig0027765:30498-30517 Msa1435660:CDS 35.0%
GTTCATTCCAAGATGATGAT+AGG - tig0027765:30600-30619 Msa1435660:CDS 35.0%
CATCTTACCTATCATCATCT+TGG + tig0027765:30610-30629 None:intergenic 35.0%
!!! TTTTTAGGTGGTTTCTAAGG+TGG - tig0027765:30695-30714 Msa1435660:CDS 35.0%
!!! TTTTAGGTGGTTTCTAAGGT+GGG - tig0027765:30696-30715 Msa1435660:CDS 35.0%
ATGTATTCATCATCCACTAC+AGG + tig0027765:30977-30996 None:intergenic 35.0%
AGAATCAAAGCCATTGTATC+AGG + tig0027765:31364-31383 None:intergenic 35.0%
!! GATACAATGGCTTTGATTCT+TGG - tig0027765:31364-31383 Msa1435660:intron 35.0%
TGATGAAATCATGTGGTACA+AGG - tig0027765:31508-31527 Msa1435660:five_prime_UTR 35.0%
GTTGCAAGCTGGATGTAATT+TGG - tig0027765:28245-28264 Msa1435660:exon 40.0%
!! TCTTTAAAAAGTGTCACGGG+AGG + tig0027765:28410-28429 None:intergenic 40.0%
TAAAGGGAACAACACACAAG+GGG - tig0027765:28574-28593 Msa1435660:CDS 40.0%
!! AGATTTCTAGCCCAAGTCAA+GGG - tig0027765:28600-28619 Msa1435660:CDS 40.0%
CAAGATACAACCCTTGACTT+GGG + tig0027765:28613-28632 None:intergenic 40.0%
ACAAGATACAACCCTTGACT+TGG + tig0027765:28614-28633 None:intergenic 40.0%
TTAAATTGTGTACCTCAGGC+CGG + tig0027765:28638-28657 None:intergenic 40.0%
CCAACAATCTTCATCACAAG+TGG + tig0027765:28734-28753 None:intergenic 40.0%
CCACTTGTGATGAAGATTGT+TGG - tig0027765:28731-28750 Msa1435660:intron 40.0%
TTGGACGAACCAGAATGATA+GGG - tig0027765:28795-28814 Msa1435660:intron 40.0%
!! TCATTCCATTTCGGGTTTGA+GGG + tig0027765:28827-28846 None:intergenic 40.0%
CAAAGGTCTCATTCCATTTC+GGG + tig0027765:28835-28854 None:intergenic 40.0%
CCAATTGGTGCAACTCTAAT+CGG - tig0027765:28911-28930 Msa1435660:intron 40.0%
!! CCTTTGAGAATTTTCTCGGA+AGG + tig0027765:28950-28969 None:intergenic 40.0%
CCTTCCGAGAAAATTCTCAA+AGG - tig0027765:28947-28966 Msa1435660:intron 40.0%
! GCAATTCTCTAGTGTCATTG+AGG - tig0027765:29054-29073 Msa1435660:CDS 40.0%
CATCCATGGATACCAATATC+TGG - tig0027765:29202-29221 Msa1435660:CDS 40.0%
GTTCCAGATATTGGTATCCA+TGG + tig0027765:29208-29227 None:intergenic 40.0%
ATATCTTCCCAACATCTTCC+AGG + tig0027765:29244-29263 None:intergenic 40.0%
!! CTACTTTCTGTTGGCTTGTT+TGG + tig0027765:29349-29368 None:intergenic 40.0%
AGGGTGATACTACTTTCTGT+TGG + tig0027765:29358-29377 None:intergenic 40.0%
ACAGAAAGTAGTATCACCCT+AGG - tig0027765:29358-29377 Msa1435660:CDS 40.0%
TTTCTTAAAAGCCGGAACAG+AGG + tig0027765:29449-29468 None:intergenic 40.0%
!! GTTCCGGCTTTTAAGAAAGA+CGG - tig0027765:29451-29470 Msa1435660:CDS 40.0%
!!! CTATGCTTTTTCCACCATCA+GGG + tig0027765:29555-29574 None:intergenic 40.0%
!!! ACTATGCTTTTTCCACCATC+AGG + tig0027765:29556-29575 None:intergenic 40.0%
! GGTGGAAAAAGCATAGTTCA+AGG - tig0027765:29559-29578 Msa1435660:CDS 40.0%
TTTGCGATCCTTCAACATGA+TGG + tig0027765:29639-29658 None:intergenic 40.0%
!! ATAGAAGCACCTGGGAAATT+TGG + tig0027765:29778-29797 None:intergenic 40.0%
!! CCTTTCTTAATAGAAGCACC+TGG + tig0027765:29787-29806 None:intergenic 40.0%
CCAGGTGCTTCTATTAAGAA+AGG - tig0027765:29784-29803 Msa1435660:CDS 40.0%
GGTGCTTCTATTAAGAAAGG+TGG - tig0027765:29787-29806 Msa1435660:CDS 40.0%
TTGCAGTGAATATCATGCCA+CGG + tig0027765:29823-29842 None:intergenic 40.0%
GATATTCACTGCAAGCTAGA+AGG - tig0027765:29829-29848 Msa1435660:CDS 40.0%
TACAACACATCCCATGCAAT+AGG + tig0027765:29856-29875 None:intergenic 40.0%
! TGTACAATTTTGAGCAGAGG+TGG - tig0027765:29872-29891 Msa1435660:CDS 40.0%
! GTACAATTTTGAGCAGAGGT+GGG - tig0027765:29873-29892 Msa1435660:CDS 40.0%
GAATTGGGAACAGGAATTGT+TGG + tig0027765:29921-29940 None:intergenic 40.0%
TATTTCCTAGGGAGTTCCTA+TGG - tig0027765:30189-30208 Msa1435660:CDS 40.0%
ATCAGTTGAATGCCAACCAT+AGG + tig0027765:30208-30227 None:intergenic 40.0%
TGCATTGCATCTCATACCAA+AGG - tig0027765:30248-30267 Msa1435660:CDS 40.0%
!! AGAGAGGTTTGTTGTGTATG+TGG - tig0027765:30308-30327 Msa1435660:CDS 40.0%
TGTATGTGGTGATTCCAATG+TGG - tig0027765:30322-30341 Msa1435660:CDS 40.0%
CTCAGTATACATCATCTCCA+TGG + tig0027765:30406-30425 None:intergenic 40.0%
GCTCTATGATAATCACTGTC+AGG + tig0027765:30552-30571 None:intergenic 40.0%
!! TTTAGGTGGTTTCTAAGGTG+GGG - tig0027765:30697-30716 Msa1435660:CDS 40.0%
AACCAAAGATCAATGGATGG+TGG - tig0027765:31019-31038 Msa1435660:intron 40.0%
TTCCACCATCCATTGATCTT+TGG + tig0027765:31024-31043 None:intergenic 40.0%
AAGAGACACAGAGATTGCAA+TGG - tig0027765:31042-31061 Msa1435660:CDS 40.0%
AGAGACACAGAGATTGCAAT+GGG - tig0027765:31043-31062 Msa1435660:CDS 40.0%
AGACCACAAGGTCAAGTATA+TGG - tig0027765:31112-31131 Msa1435660:intron 40.0%
AAGCCATATACTTGACCTTG+TGG + tig0027765:31118-31137 None:intergenic 40.0%
AAGGTCAAGTATATGGCTTC+AGG - tig0027765:31119-31138 Msa1435660:intron 40.0%
! TTTTGACGAACCTGAAAGAG+TGG - tig0027765:31183-31202 Msa1435660:intron 40.0%
TTTACGCATTCCACTCTTTC+AGG + tig0027765:31196-31215 None:intergenic 40.0%
CTTCAACAGGATACCGTAAA+AGG + tig0027765:31294-31313 None:intergenic 40.0%
TTTACGGTATCCTGTTGAAG+TGG - tig0027765:31294-31313 Msa1435660:intron 40.0%
TTACGGTATCCTGTTGAAGT+GGG - tig0027765:31295-31314 Msa1435660:intron 40.0%
GAAGTGTGACATCACTTTCA+GGG - tig0027765:31326-31345 Msa1435660:intron 40.0%
TAGGACTAGGTGGTAAGTAT+GGG + tig0027765:31412-31431 None:intergenic 40.0%
ATAGTGTATGAACGAGCAAG+TGG + tig0027765:31552-31571 None:intergenic 40.0%
ACTTGCTCGTTCATACACTA+TGG - tig0027765:31551-31570 Msa1435660:five_prime_UTR 40.0%
AAAATGCCTCAGCTGCTACA+TGG - tig0027765:28189-28208 Msa1435660:three_prime_UTR 45.0%
AAATGCCTCAGCTGCTACAT+GGG - tig0027765:28190-28209 Msa1435660:three_prime_UTR 45.0%
!! GAAGTTGATAGGTTGCAAGC+TGG - tig0027765:28234-28253 Msa1435660:exon 45.0%
!! GAGATTTCTAGCCCAAGTCA+AGG - tig0027765:28599-28618 Msa1435660:CDS 45.0%
TCAAGGGTTGTATCTTGTGC+CGG - tig0027765:28616-28635 Msa1435660:CDS 45.0%
GTTGGACGAACCAGAATGAT+AGG - tig0027765:28794-28813 Msa1435660:intron 45.0%
! GGGTTGATTCCCTATCATTC+TGG + tig0027765:28807-28826 None:intergenic 45.0%
ATCAACCCTCAAACCCGAAA+TGG - tig0027765:28819-28838 Msa1435660:intron 45.0%
!! CTCATTCCATTTCGGGTTTG+AGG + tig0027765:28828-28847 None:intergenic 45.0%
CCGATTAGAGTTGCACCAAT+TGG + tig0027765:28914-28933 None:intergenic 45.0%
!!! ATTTTAGAACCGCCTGGTAC+AGG + tig0027765:29022-29041 None:intergenic 45.0%
!!! ACACGAATTTTAGAACCGCC+TGG + tig0027765:29028-29047 None:intergenic 45.0%
GTACGCTGAACTCCCAAAAA+TGG + tig0027765:29115-29134 None:intergenic 45.0%
TTCGATCAGAGACAAGGTTG+TGG - tig0027765:29139-29158 Msa1435660:CDS 45.0%
TCCATGGATGAACACTGTCA+GGG + tig0027765:29192-29211 None:intergenic 45.0%
ATCCATGGATGAACACTGTC+AGG + tig0027765:29193-29212 None:intergenic 45.0%
CTTGTTTGGGTCCCTAATCA+AGG + tig0027765:29335-29354 None:intergenic 45.0%
!! GTTGATGGCCATCATGTTGA+AGG - tig0027765:29628-29647 Msa1435660:CDS 45.0%
!! GGTGGTATTGATCTGTGTGA+TGG - tig0027765:29682-29701 Msa1435660:CDS 45.0%
ATATCATGCCACGGTTCTCT+AGG + tig0027765:29814-29833 None:intergenic 45.0%
AGCTAGAAGGACCTATTGCA+TGG - tig0027765:29842-29861 Msa1435660:CDS 45.0%
GCTAGAAGGACCTATTGCAT+GGG - tig0027765:29843-29862 Msa1435660:CDS 45.0%
CAAGCTCTTGAATTGGGAAC+AGG + tig0027765:29930-29949 None:intergenic 45.0%
CAGAAGAAGATGGCGAAACT+TGG - tig0027765:29986-30005 Msa1435660:CDS 45.0%
AGAAACAGCACCACCATCAA+TGG + tig0027765:30031-30050 None:intergenic 45.0%
AGATTGGCAAAGAAGGACCA+TGG - tig0027765:30386-30405 Msa1435660:CDS 45.0%
TGATAATCACTGTCAGGCTC+TGG + tig0027765:30546-30565 None:intergenic 45.0%
!! TTAGGTGGTTTCTAAGGTGG+GGG - tig0027765:30698-30717 Msa1435660:CDS 45.0%
GGCATTTCCCGTAAGAAGAT+TGG - tig0027765:30788-30807 Msa1435660:CDS 45.0%
GATGTGGATGCTAGATGATG+AGG + tig0027765:31079-31098 None:intergenic 45.0%
CATCTAGCATCCACATCCAA+TGG - tig0027765:31082-31101 Msa1435660:intron 45.0%
TCTTTGTGAGCCATTGGATG+TGG + tig0027765:31095-31114 None:intergenic 45.0%
TTGTGGTCTTTGTGAGCCAT+TGG + tig0027765:31101-31120 None:intergenic 45.0%
AATGGCTCACAAAGACCACA+AGG - tig0027765:31100-31119 Msa1435660:intron 45.0%
!! ATGGCTTCAGGAGAGCATTA+TGG - tig0027765:31131-31150 Msa1435660:intron 45.0%
!! TTTTGAACCGTGTTGCTGAG+AGG - tig0027765:31218-31237 Msa1435660:intron 45.0%
TCCAATTCCTCTCAGCAACA+CGG + tig0027765:31228-31247 None:intergenic 45.0%
! GGAAGTGTGACATCACTTTC+AGG - tig0027765:31325-31344 Msa1435660:intron 45.0%
CTAGGACTAGGTGGTAAGTA+TGG + tig0027765:31413-31432 None:intergenic 45.0%
!!! AAAAAATTGAATTTTAATTT+AGG - tig0027765:28118-28137 Msa1435660:three_prime_UTR 5.0%
GCTGCTACATGGGACACTTA+AGG - tig0027765:28200-28219 Msa1435660:three_prime_UTR 50.0%
GAAGATCATCGTCCTGTACC+AGG - tig0027765:29007-29026 Msa1435660:intron 50.0%
TCCCTGACAGTGTTCATCCA+TGG - tig0027765:29188-29207 Msa1435660:CDS 50.0%
TTATGTCCGCGTAACCCTGA+TGG - tig0027765:29538-29557 Msa1435660:CDS 50.0%
TTTCCACCATCAGGGTTACG+CGG + tig0027765:29547-29566 None:intergenic 50.0%
CAGAGGTGGGAAAAGCAAGT+CGG - tig0027765:29886-29905 Msa1435660:CDS 50.0%
AGAGGTGGGAAAAGCAAGTC+GGG - tig0027765:29887-29906 Msa1435660:CDS 50.0%
TCCTAGGGAGTTCCTATGGT+TGG - tig0027765:30193-30212 Msa1435660:CDS 50.0%
GCAAGATTGAAGCTGGAGAG+AGG - tig0027765:30292-30311 Msa1435660:CDS 50.0%
GTGATTCCAATGTGGCCAGA+AGG - tig0027765:30330-30349 Msa1435660:CDS 50.0%
GGTATACCTTCTGGCCACAT+TGG + tig0027765:30339-30358 None:intergenic 50.0%
GCACTCTCAGGTATACCTTC+TGG + tig0027765:30348-30367 None:intergenic 50.0%
! ACCTGAGAGTGCATCAGTTC+AGG - tig0027765:30356-30375 Msa1435660:CDS 50.0%
!! AGGTGGGGGAGTGTTTCATA+TGG - tig0027765:30712-30731 Msa1435660:CDS 50.0%
! ACCGTGTTGCTGAGAGGAAT+TGG - tig0027765:31224-31243 Msa1435660:intron 50.0%
CCATCTTCACCCACTTCAAC+AGG + tig0027765:31307-31326 None:intergenic 50.0%
CCTGTTGAAGTGGGTGAAGA+TGG - tig0027765:31304-31323 Msa1435660:intron 50.0%
AGGTGGTAAGTATGGGAGGA+AGG + tig0027765:31405-31424 None:intergenic 50.0%
GACTAGGTGGTAAGTATGGG+AGG + tig0027765:31409-31428 None:intergenic 50.0%
TGTGCTAGCTAGCTAGGACT+AGG + tig0027765:31425-31444 None:intergenic 50.0%
! AAGCACTGTGCTAGCTAGCT+AGG + tig0027765:31431-31450 None:intergenic 50.0%
CTAGCTAGCACAGTGCTTGA+CGG - tig0027765:31433-31452 Msa1435660:CDS 50.0%
GGGGTCGGGAGAGGAAATAA+TGG + tig0027765:28088-28107 None:intergenic 55.0%
CAAAATGTAGGGGTCGGGAG+AGG + tig0027765:28097-28116 None:intergenic 55.0%
AGTGTCCCATGTAGCAGCTG+AGG + tig0027765:28198-28217 None:intergenic 55.0%
! TTGTATCTTGTGCCGGCCTG+AGG - tig0027765:28623-28642 Msa1435660:CDS 55.0%
GATCATCGTCCTGTACCAGG+CGG - tig0027765:29010-29029 Msa1435660:intron 55.0%
TGTCAGGGACATGAGCGTCT+TGG + tig0027765:29177-29196 None:intergenic 55.0%
GGACGACAGAACCTCTGTTC+CGG - tig0027765:29435-29454 Msa1435660:CDS 55.0%
AAGGTGGTCCTAGAGAACCG+TGG - tig0027765:29803-29822 Msa1435660:CDS 55.0%
GCCAACCATAGGAACTCCCT+AGG + tig0027765:30197-30216 None:intergenic 55.0%
GCCTGAACTGATGCACTCTC+AGG + tig0027765:30360-30379 None:intergenic 55.0%
GCTAGCTAGCTAGGACTAGG+TGG + tig0027765:31422-31441 None:intergenic 55.0%
AGTGCTTGACGGCCAAGACA+AGG - tig0027765:31444-31463 Msa1435660:CDS 55.0%
TGTCCGCGTAACCCTGATGG+TGG - tig0027765:29541-29560 Msa1435660:CDS 60.0%
! TCAGGCTCTGGTTGCTCTGG+AGG + tig0027765:30534-30553 None:intergenic 60.0%
! CTGTCAGGCTCTGGTTGCTC+TGG + tig0027765:30537-30556 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0027765 gene 28059 31614 28059 ID=Msa1435660;Name=Msa1435660
tig0027765 mRNA 28059 31614 28059 ID=Msa1435660-mRNA-1;Parent=Msa1435660;Name=Msa1435660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=133|1|1|1|1|1|4|188|825
tig0027765 exon 28059 28692 28059 ID=Msa1435660-mRNA-1:exon:9807;Parent=Msa1435660-mRNA-1
tig0027765 exon 29052 30927 29052 ID=Msa1435660-mRNA-1:exon:9806;Parent=Msa1435660-mRNA-1
tig0027765 exon 31029 31094 31029 ID=Msa1435660-mRNA-1:exon:9805;Parent=Msa1435660-mRNA-1
tig0027765 exon 31392 31614 31392 ID=Msa1435660-mRNA-1:exon:9804;Parent=Msa1435660-mRNA-1
tig0027765 five_prime_UTR 31482 31614 31482 ID=Msa1435660-mRNA-1:five_prime_utr;Parent=Msa1435660-mRNA-1
tig0027765 CDS 31392 31481 31392 ID=Msa1435660-mRNA-1:cds;Parent=Msa1435660-mRNA-1
tig0027765 CDS 31029 31094 31029 ID=Msa1435660-mRNA-1:cds;Parent=Msa1435660-mRNA-1
tig0027765 CDS 29052 30927 29052 ID=Msa1435660-mRNA-1:cds;Parent=Msa1435660-mRNA-1
tig0027765 CDS 28247 28692 28247 ID=Msa1435660-mRNA-1:cds;Parent=Msa1435660-mRNA-1
tig0027765 three_prime_UTR 28059 28246 28059 ID=Msa1435660-mRNA-1:three_prime_utr;Parent=Msa1435660-mRNA-1
Gene Sequence

>Msa1435660

ATGCCTCAGCTGCTACATGGGACACTTAAGGTAACCATAAGTGAAGTTGATAGGTTGCAAGCTGGATGTAATTTGGATTTCTGTCGAAAGGGAACAACACACAAGGGGAAGAGATTTCTAGCCCAAGTCAAGGGTTGTATCTTGTGCCGGCCTGAGATTGTTGGGACAAGACTCTATGCAACAGTTGATTTAGATAAAGCAAGGGTTGGACGAACCAGAATGATAGGGAATCAACCCTCAAACCCGAAATGGAATGAGACCTTTGAAATCTATTGTGCTCATTACATATCAAATATTGTATTCACTGTCAAAGATGATAATCCAATTGGTGCAACTCTAATCGGAAGAGCTTATATCCCTTCCGAGAAAATTCTCAAAGGACCTATAATTGACAGATGGGTTGAGATATTAGACGAAGAAGATCATCGTCCTGTACCAGGCGGTTCTAAAATTCGTGTCAGCATGCAATTCTCTAGTGTCATTGAGGATAGAAAATGTCTATGGTCTCAAGGAATACACATGCCATTTTTGGGAGTTCAGCGTACATTTTTCGATCAGAGACAAGGTTGTGGTGTTACTTTATACCAAGACGCTCATGTCCCTGACAGTGTTCATCCATGGATACCAATATCTGGAACAAAATATTATGTTCCTGGAAGATGTTGGGAAGATATTTACAATGCCATTATGAATGCTAAAGTCTTTATTTACATAACTGGTTGGTCTGTTCATACAGAAATAACCTTGATTAGGGACCCAAACAAGCCAACAGAAAGTAGTATCACCCTAGGAGAAATACTCAAGAAAAAAGCCAATGAAGGTGTTAATGTTCTTGTGCTTGTTTGGGACGACAGAACCTCTGTTCCGGCTTTTAAGAAAGACGGTTTGATGGGAACACATGACCAAGAAACTGCAGAGTATTTTAAAAACACAAAGGTACGTTGTGTTTTATGTCCGCGTAACCCTGATGGTGGAAAAAGCATAGTTCAAGGTTTTGAAACTTCAACTATGTTCACTCATCACCAAAAGACTATAGTTGTTGATGGCCATCATGTTGAAGGATCGCAAAAGCGAACAGTTATCAGTTTCATAGGTGGTATTGATCTGTGTGATGGAAGATATGATACAATGGAACATCCTTTGTTTTCAACTTTGAACACAATCCATCATGATGATTTTCATCAACCAAATTTCCCAGGTGCTTCTATTAAGAAAGGTGGTCCTAGAGAACCGTGGCATGATATTCACTGCAAGCTAGAAGGACCTATTGCATGGGATGTGTTGTACAATTTTGAGCAGAGGTGGGAAAAGCAAGTCGGGAAGCAATTCCAACAATTCCTGTTCCCAATTCAAGAGCTTGATAGAATTCTAATCCATCCTTCTGATGCAATGTTATCAGAAGAAGATGGCGAAACTTGGAGCGTTCAGTTGTTTAGATCCATTGATGGTGGTGCTGTTTCTAACTTCCCTCAAGCACCAAATGAAGTTATTGAATTAGGCCTTGTTAGTGGAAAAGACAACATCATTGATAGAAGCATTCAAGATGCCTACATAAATGCTATACGAAGAGCAAAAAACTTCATCTACATTGAAAATCAGTATTTCCTAGGGAGTTCCTATGGTTGGCATTCAACTGATATTAAAGTTGAGGATATTGGTGCATTGCATCTCATACCAAAGGAGTTGTCATTGAAGATTGTTAGCAAGATTGAAGCTGGAGAGAGGTTTGTTGTGTATGTGGTGATTCCAATGTGGCCAGAAGGTATACCTGAGAGTGCATCAGTTCAGGCAATATTAGATTGGCAAAGAAGGACCATGGAGATGATGTATACTGAGATTGCTGATGCCATAAAAAAGAAGAATGTTGAAGCACACCCTCGTGATTATTTGACATTTTATTGTCTTGGTAAAAGGGAGAGTAAGAAAGATGGAGAGTACACTCCTCCAGAGCAACCAGAGCCTGACAGTGATTATCATAGAGCACAAAAGTCTAGAAGATTCATGATCTATGTTCATTCCAAGATGATGATAGTGGATGATGAATACATAGTGATTGGTTCAGCAAACATAAACCAAAGATCAATGGATGGTGGAAGAGACACAGAGATTGCAATGGGTGCATTTCAACCTCATCATCTAGCATCCACATCCAATGGCTCACAAAGACCACAAGGTCAAGTATATGGCTTCAGGAGAGCATTATGGTATGAACATATTGGAGACAACAGTGATGATTTTGACGAACCTGAAAGAGTGGAATGCGTAAAACTTTTGAACCGTGTTGCTGAGAGGAATTGGAAATTATACTGTGATGATGCATTAGATGAAAGTGTAAATACTCACCTTTTACGGTATCCTGTTGAAGTGGGTGAAGATGGAAGTGTGACATCACTTTCAGGGATGCAATATTTTCCTGATACAATGGCTTTGATTCTTGGTTCAAAATCTGATTACCTTCCTCCCATACTTACCACCTAG
Protein sequence

>Msa1435660

MPQLLHGTLKVTISEVDRLQAGCNLDFCRKGTTHKGKRFLAQVKGCILCRPEIVGTRLYATVDLDKARVGRTRMIGNQPSNPKWNETFEIYCAHYISNIVFTVKDDNPIGATLIGRAYIPSEKILKGPIIDRWVEILDEEDHRPVPGGSKIRVSMQFSSVIEDRKCLWSQGIHMPFLGVQRTFFDQRQGCGVTLYQDAHVPDSVHPWIPISGTKYYVPGRCWEDIYNAIMNAKVFIYITGWSVHTEITLIRDPNKPTESSITLGEILKKKANEGVNVLVLVWDDRTSVPAFKKDGLMGTHDQETAEYFKNTKVRCVLCPRNPDGGKSIVQGFETSTMFTHHQKTIVVDGHHVEGSQKRTVISFIGGIDLCDGRYDTMEHPLFSTLNTIHHDDFHQPNFPGASIKKGGPREPWHDIHCKLEGPIAWDVLYNFEQRWEKQVGKQFQQFLFPIQELDRILIHPSDAMLSEEDGETWSVQLFRSIDGGAVSNFPQAPNEVIELGLVSGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSYGWHSTDIKVEDIGALHLIPKELSLKIVSKIEAGERFVVYVVIPMWPEGIPESASVQAILDWQRRTMEMMYTEIADAIKKKNVEAHPRDYLTFYCLGKRESKKDGEYTPPEQPEPDSDYHRAQKSRRFMIYVHSKMMIVDDEYIVIGSANINQRSMDGGRDTEIAMGAFQPHHLASTSNGSQRPQGQVYGFRRALWYEHIGDNSDDFDEPERVECVKLLNRVAERNWKLYCDDALDESVNTHLLRYPVEVGEDGSVTSLSGMQYFPDTMALILGSKSDYLPPILTT*