Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438540 | RHN69381.1 | 98.000 | 50 | 1 | 0 | 1 | 50 | 1 | 50 | 9.31e-27 | 105 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438540 | A0A396IXH4 | 98.000 | 50 | 1 | 0 | 1 | 50 | 1 | 50 | 4.45e-27 | 105 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1015930 | Msa1438540 | 0.832406 | 9.626606e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1438540 | MtrunA17_Chr3g0124091 | 98.000 | 50 | 1 | 0 | 1 | 50 | 1 | 50 | 8.56e-31 | 105 |
| Msa1438540 | MtrunA17_Chr3g0124141 | 92.000 | 50 | 4 | 0 | 1 | 50 | 1 | 50 | 1.55e-27 | 97.1 |
| Msa1438540 | MtrunA17_Chr3g0124111 | 86.000 | 50 | 7 | 0 | 1 | 50 | 1 | 50 | 7.93e-27 | 95.5 |
| Msa1438540 | MtrunA17_Chr3g0124131 | 80.000 | 50 | 10 | 0 | 1 | 50 | 1 | 50 | 3.27e-26 | 89.4 |
| Msa1438540 | MtrunA17_Chr3g0124061 | 82.000 | 50 | 9 | 0 | 1 | 50 | 1 | 50 | 6.93e-25 | 90.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 1 sgRNAs with CRISPR-Local
Find 8 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAGGTTGTATCTTCCAAC+AGG | 0.413782 | tig0028583:-37 | Msa1438540:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCTTTTGTTGTTTCCTGT+TGG | + | tig0028583:132-151 | None:intergenic | 30.0% |
| GCATAAGCAGGTATATGTAA+TGG | + | tig0028583:21-40 | None:intergenic | 35.0% | |
| ACTTGACAGATTCAACGTTT+CGG | + | tig0028583:77-96 | None:intergenic | 35.0% | |
| CTTGACAGATTCAACGTTTC+GGG | + | tig0028583:76-95 | None:intergenic | 40.0% | |
| ! | CCTTGCAAACAAGTTTTTCG+TGG | + | tig0028583:100-119 | None:intergenic | 40.0% |
| ! | CCACGAAAAACTTGTTTGCA+AGG | - | tig0028583:97-116 | Msa1438540:CDS | 40.0% |
| CAGCTGAAGTTTGCATAAGC+AGG | + | tig0028583:33-52 | None:intergenic | 45.0% | |
| GCAAGGTTGTATCTTCCAAC+AGG | - | tig0028583:114-133 | Msa1438540:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0028583 | gene | 12 | 161 | 12 | ID=Msa1438540;Name=Msa1438540 |
| tig0028583 | mRNA | 12 | 161 | 12 | ID=Msa1438540-mRNA-1;Parent=Msa1438540;Name=Msa1438540-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|50 |
| tig0028583 | exon | 12 | 161 | 12 | ID=Msa1438540-mRNA-1:exon:11326;Parent=Msa1438540-mRNA-1 |
| tig0028583 | CDS | 12 | 161 | 12 | ID=Msa1438540-mRNA-1:cds;Parent=Msa1438540-mRNA-1 |
| Gene Sequence |
| Protein sequence |
