AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1015930


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1015930 MtrunA17_Chr7g0236571 76.471 51 12 0 1 51 600 650 7.35e-22 87.4
Msa1015930 MtrunA17_Chr7g0236471 76.471 51 12 0 1 51 605 655 3.44e-20 82.4
Msa1015930 MtrunA17_Chr7g0236551 76.471 51 12 0 1 51 753 803 5.17e-20 82.0
Msa1015930 MtrunA17_Chr7g0236341 78.431 51 7 1 1 51 782 828 1.82e-19 80.5
Msa1015930 MtrunA17_Chr7g0236511 72.549 51 14 0 1 51 745 795 9.88e-19 78.6
Msa1015930 MtrunA17_Chr7g0236531 70.588 51 15 0 1 51 303 353 3.83e-18 76.3
Msa1015930 MtrunA17_Chr7g0236691 68.627 51 16 0 1 51 765 815 2.85e-16 71.2
Msa1015930 MtrunA17_Chr7g0236201 59.615 52 20 1 1 51 734 785 2.60e-14 65.9
Msa1015930 MtrunA17_Chr7g0236641 60.784 51 20 0 1 51 790 840 5.21e-14 65.1
Msa1015930 MtrunA17_Chr7g0236161 71.053 38 11 0 1 38 715 752 2.57e-13 63.2
Msa1015930 MtrunA17_Chr7g0236561 62.745 51 18 1 1 51 770 819 2.90e-13 62.8
Msa1015930 MtrunA17_Chr7g0236121 71.053 38 11 0 1 38 768 805 3.39e-13 62.8
Msa1015930 MtrunA17_Chr7g0236651 68.182 44 14 0 1 44 750 793 6.64e-13 62.0
Msa1015930 MtrunA17_Chr4g0055011 60.784 51 20 0 1 51 336 386 1.92e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCAACATATTCTCCATC+AGG 0.408760 7_1:+30842730 None:intergenic
ATGATTGTCAATCAAATTAG+TGG 0.418818 7_1:+30840260 None:intergenic
CAAGAATAACATGTAACAAA+TGG 0.464556 7_1:+30840321 None:intergenic
AGGATGAATGCAGGTTTACT+AGG 0.474164 7_1:+30842750 None:intergenic
TCCACATACGTCTGTAATAC+CGG 0.478739 7_1:+30842662 None:intergenic
ATATGTTCTATTAATACTGC+CGG 0.533049 7_1:-30842681 Msa1015930:CDS
TAAACCTGCATTCATCCTGA+TGG 0.539083 7_1:-30842745 Msa1015930:CDS
TTCTCCATCAGGATGAATGC+AGG 0.539314 7_1:+30842741 None:intergenic
AATGCAGGTTTACTAGGAGG+AGG 0.559449 7_1:+30842756 None:intergenic
GAATAACATGTAACAAATGG+AGG 0.569599 7_1:+30840324 None:intergenic
AATAACATGTAACAAATGGA+GGG 0.612635 7_1:+30840325 None:intergenic
AATTTCTATGCTTAACAGTG+AGG 0.622691 7_1:-30842790 Msa1015930:CDS
ATGAATGCAGGTTTACTAGG+AGG 0.660515 7_1:+30842753 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTCAATTAATATATATAT+TGG - chr7_1:30841695-30841714 Msa1015930:intron 10.0%
!! AAAAATAATCAAAATTATTG+TGG + chr7_1:30841886-30841905 None:intergenic 10.0%
!!! ATAATAAAGCAAAATAATTT+TGG + chr7_1:30842094-30842113 None:intergenic 10.0%
!!! GTGATAATTTAACTTTATTT+AGG - chr7_1:30841197-30841216 Msa1015930:intron 15.0%
!!! TAATTTTGTTAAATGGAATA+TGG - chr7_1:30841325-30841344 Msa1015930:intron 15.0%
!!! GATTTTCATAACTAATTTTA+AGG + chr7_1:30841384-30841403 None:intergenic 15.0%
!! TAGATAAATAACACAAACAA+TGG + chr7_1:30840645-30840664 None:intergenic 20.0%
!! ATTATCATGTAGACTATATA+TGG - chr7_1:30840683-30840702 Msa1015930:intron 20.0%
!! GTTGTTATCTTCATATTATA+AGG + chr7_1:30841033-30841052 None:intergenic 20.0%
!! TGAAATCATATGAAAAAGTA+AGG + chr7_1:30841298-30841317 None:intergenic 20.0%
!!! AGCAAACTAATTTTGTTAAA+TGG - chr7_1:30841318-30841337 Msa1015930:intron 20.0%
!!! ATTTTTTATTACACCAATTC+AGG - chr7_1:30841723-30841742 Msa1015930:intron 20.0%
!!! GCAACTTTTAAAAAAAATGA+AGG + chr7_1:30842350-30842369 None:intergenic 20.0%
!! TTTAGATCTGTAATCTAAAA+AGG + chr7_1:30842479-30842498 None:intergenic 20.0%
!! CTAAAGAAAAAACATAATTG+AGG + chr7_1:30842726-30842745 None:intergenic 20.0%
!!! CTCAATTATGTTTTTTCTTT+AGG - chr7_1:30842724-30842743 Msa1015930:CDS 20.0%
!! TTTGATGGAAATAAAAATTC+TGG + chr7_1:30842783-30842802 None:intergenic 20.0%
! ATATGTTCTATTAATACTGC+CGG - chr7_1:30840391-30840410 Msa1015930:intron 25.0%
! TTTGTGAAAAAACAAAACTC+TGG - chr7_1:30840500-30840519 Msa1015930:intron 25.0%
! ACATAAAGACATGTATAGTA+TGG - chr7_1:30840539-30840558 Msa1015930:intron 25.0%
! AATAACACAAACAATGGTAA+AGG + chr7_1:30840639-30840658 None:intergenic 25.0%
! ATAGTATAGTTTCTCTTTAG+AGG - chr7_1:30840718-30840737 Msa1015930:intron 25.0%
! TAGTATAGTTTCTCTTTAGA+GGG - chr7_1:30840719-30840738 Msa1015930:intron 25.0%
!!! AGAAAATTTTGTGTTGATTG+TGG - chr7_1:30840818-30840837 Msa1015930:intron 25.0%
! TAATATGAAGATAACAACAG+TGG - chr7_1:30841035-30841054 Msa1015930:intron 25.0%
!!! TTCTGAATATCCGTTTTATT+CGG - chr7_1:30841163-30841182 Msa1015930:intron 25.0%
! TATTTGTCAACCGAATAAAA+CGG + chr7_1:30841176-30841195 None:intergenic 25.0%
! AAATGGAATATGGAAGAATT+CGG - chr7_1:30841335-30841354 Msa1015930:intron 25.0%
! TTCAAATCAATTACAGCATT+CGG - chr7_1:30841404-30841423 Msa1015930:intron 25.0%
!! CTTCTTTAGGAGAAATAATA+GGG - chr7_1:30841448-30841467 Msa1015930:intron 25.0%
! AAGTTACCAATTCAATATCT+TGG - chr7_1:30841483-30841502 Msa1015930:intron 25.0%
! AGTTACCAATTCAATATCTT+GGG - chr7_1:30841484-30841503 Msa1015930:intron 25.0%
!! TGTCTACATTTTAAGCATAA+AGG - chr7_1:30841669-30841688 Msa1015930:intron 25.0%
!! TCTTTTGATAAAAATAGCAG+AGG - chr7_1:30842135-30842154 Msa1015930:intron 25.0%
!! CTTTTGATAAAAATAGCAGA+GGG - chr7_1:30842136-30842155 Msa1015930:intron 25.0%
! ATCTAAAAAGGATCACAATT+AGG + chr7_1:30842467-30842486 None:intergenic 25.0%
! GAGCAAATCAACATTATTTA+TGG - chr7_1:30842509-30842528 Msa1015930:intron 25.0%
! TCAACATTATTTATGGTACA+TGG - chr7_1:30842516-30842535 Msa1015930:intron 25.0%
!! TTTTGTAACATTTCTTGCTA+TGG - chr7_1:30842573-30842592 Msa1015930:intron 25.0%
! TTGTGACATTGTTTAAATCT+AGG - chr7_1:30842598-30842617 Msa1015930:intron 25.0%
! AATAACATGTAACAAATGGA+GGG + chr7_1:30842750-30842769 None:intergenic 25.0%
! CAAGAATAACATGTAACAAA+TGG + chr7_1:30842754-30842773 None:intergenic 25.0%
AATTTCTATGCTTAACAGTG+AGG - chr7_1:30840282-30840301 Msa1015930:CDS 30.0%
AATTCAACATATTCTCCATC+AGG + chr7_1:30840345-30840364 None:intergenic 30.0%
! CATATACTTCGATTTTCTTC+AGG + chr7_1:30840376-30840395 None:intergenic 30.0%
GAAAAAACAAAACTCTGGTT+TGG - chr7_1:30840505-30840524 Msa1015930:intron 30.0%
! TACGACAGCATTGAAAATTA+TGG - chr7_1:30840583-30840602 Msa1015930:intron 30.0%
AGAAACTATACTATAGACCA+TGG + chr7_1:30840712-30840731 None:intergenic 30.0%
GAACAAAATCTGGAAAGTTA+GGG - chr7_1:30840904-30840923 Msa1015930:intron 30.0%
TAATATAACCTCCACGTATA+AGG - chr7_1:30840981-30841000 Msa1015930:intron 30.0%
!!! AACAGTGGAAGTTTTTAGTT+TGG - chr7_1:30841050-30841069 Msa1015930:intron 30.0%
!!! ACAGTGGAAGTTTTTAGTTT+GGG - chr7_1:30841051-30841070 Msa1015930:intron 30.0%
! TTAACTTTATTTAGGAGCTG+AGG - chr7_1:30841205-30841224 Msa1015930:intron 30.0%
TATTTCTCCTAAAGAAGCAA+TGG + chr7_1:30841445-30841464 None:intergenic 30.0%
! GCTTCTTTAGGAGAAATAAT+AGG - chr7_1:30841447-30841466 Msa1015930:intron 30.0%
CAAAACCCAAGATATTGAAT+TGG + chr7_1:30841492-30841511 None:intergenic 30.0%
! CAATTCAATATCTTGGGTTT+TGG - chr7_1:30841490-30841509 Msa1015930:intron 30.0%
CAAGGATTATGTTGAAAGTT+TGG - chr7_1:30841522-30841541 Msa1015930:intron 30.0%
AGACAATCGAGTATTAAAGA+TGG + chr7_1:30841654-30841673 None:intergenic 30.0%
CATTGTTGTAAAAACATGCT+TGG + chr7_1:30842273-30842292 None:intergenic 30.0%
!! CAAGCATGTTTTTACAACAA+TGG - chr7_1:30842271-30842290 Msa1015930:intron 30.0%
CTTGAGAAATCTGTATTTGA+TGG - chr7_1:30842304-30842323 Msa1015930:intron 30.0%
ATTCAAATCCTATGATGCAT+AGG + chr7_1:30842410-30842429 None:intergenic 30.0%
CTCATTAAAAACTCCATTTG+TGG + chr7_1:30842646-30842665 None:intergenic 30.0%
! CACAAATGGAGTTTTTAATG+AGG - chr7_1:30842644-30842663 Msa1015930:intron 30.0%
GAATAACATGTAACAAATGG+AGG + chr7_1:30842751-30842770 None:intergenic 30.0%
!!! TAGTGGAGTTGATATTTTGA+TGG + chr7_1:30842798-30842817 None:intergenic 30.0%
ACAAACAATGGTAAAGGCAA+CGG + chr7_1:30840633-30840652 None:intergenic 35.0%
AGTGTAAGTGAGCATTTGTA+GGG - chr7_1:30840841-30840860 Msa1015930:intron 35.0%
CGAACAAAATCTGGAAAGTT+AGG - chr7_1:30840903-30840922 Msa1015930:intron 35.0%
!! TATCTCAGTTGATGAATCGA+AGG + chr7_1:30840938-30840957 None:intergenic 35.0%
AAGTAAGGAATCCCATAGTT+GGG + chr7_1:30841283-30841302 None:intergenic 35.0%
AAAGTAAGGAATCCCATAGT+TGG + chr7_1:30841284-30841303 None:intergenic 35.0%
!! AATATCTTGGGTTTTGGTCA+AGG - chr7_1:30841496-30841515 Msa1015930:intron 35.0%
CGAGTATTAAAGATGGTAAC+AGG + chr7_1:30841647-30841666 None:intergenic 35.0%
!!! TAGTTTTGATCTCTGAAGCA+TGG - chr7_1:30841915-30841934 Msa1015930:intron 35.0%
!!! AGTTTTGATCTCTGAAGCAT+GGG - chr7_1:30841916-30841935 Msa1015930:intron 35.0%
! CAACATGATCATAGAAGTGT+GGG - chr7_1:30842187-30842206 Msa1015930:intron 35.0%
AATCCTATGATGCATAGGTA+CGG + chr7_1:30842405-30842424 None:intergenic 35.0%
AAAAAGGATCACAATTAGGC+AGG + chr7_1:30842463-30842482 None:intergenic 35.0%
! GCAGAATGATGGGAATAAAT+TGG - chr7_1:30842538-30842557 Msa1015930:intron 35.0%
!! ATGGGAATAAATTGGCTTGT+TGG - chr7_1:30842546-30842565 Msa1015930:intron 35.0%
ATGAATGCAGGTTTACTAGG+AGG + chr7_1:30840322-30840341 None:intergenic 40.0%
AGGATGAATGCAGGTTTACT+AGG + chr7_1:30840325-30840344 None:intergenic 40.0%
TAAACCTGCATTCATCCTGA+TGG - chr7_1:30840327-30840346 Msa1015930:CDS 40.0%
TCCACATACGTCTGTAATAC+CGG + chr7_1:30840413-30840432 None:intergenic 40.0%
ATGTGGAAGATAGCACACTA+AGG - chr7_1:30840426-30840445 Msa1015930:intron 40.0%
CACACTAAGGACTCTAACAA+GGG - chr7_1:30840439-30840458 Msa1015930:intron 40.0%
TAGACTATATATGGCTGCCA+TGG - chr7_1:30840692-30840711 Msa1015930:intron 40.0%
GAGTGTAAGTGAGCATTTGT+AGG - chr7_1:30840840-30840859 Msa1015930:intron 40.0%
AAGATGTATGCCTTCGTTTC+TGG - chr7_1:30840868-30840887 Msa1015930:intron 40.0%
! CAGATTTTGTTCGTGTGCAT+GGG + chr7_1:30840896-30840915 None:intergenic 40.0%
CAACAACACAACCTTATACG+TGG + chr7_1:30840995-30841014 None:intergenic 40.0%
AGGTGCTCGATATTGGAAAA+AGG - chr7_1:30841121-30841140 Msa1015930:intron 40.0%
GGTGCTCGATATTGGAAAAA+GGG - chr7_1:30841122-30841141 Msa1015930:intron 40.0%
! ATTGGAAAAAGGGTGCAAAG+TGG - chr7_1:30841132-30841151 Msa1015930:intron 40.0%
ATTCATCAAAACCTTGAGGC+AGG + chr7_1:30841247-30841266 None:intergenic 40.0%
GGCAATTCATCAAAACCTTG+AGG + chr7_1:30841251-30841270 None:intergenic 40.0%
! TTTCGTGCCATTGCTTCTTT+AGG - chr7_1:30841435-30841454 Msa1015930:intron 40.0%
GGAGAAATAATAGGGTAGAG+AGG - chr7_1:30841456-30841475 Msa1015930:intron 40.0%
CAGCATTACTGTACCTGAAT+TGG + chr7_1:30841739-30841758 None:intergenic 40.0%
AACCAGTGTGTAATCTGTAG+TGG - chr7_1:30841803-30841822 Msa1015930:intron 40.0%
ACCAGTGTGTAATCTGTAGT+GGG - chr7_1:30841804-30841823 Msa1015930:intron 40.0%
!! ACTTGTTGTTTCAGCGTAGT+TGG - chr7_1:30842035-30842054 Msa1015930:intron 40.0%
GCAACATGATCATAGAAGTG+TGG - chr7_1:30842186-30842205 Msa1015930:intron 40.0%
AAAGTTGCCGTATTACGTAC+CGG - chr7_1:30842362-30842381 Msa1015930:intron 40.0%
ATCCTATGATGCATAGGTAC+GGG + chr7_1:30842404-30842423 None:intergenic 40.0%
TATGGTACATGGCAGAATGA+TGG - chr7_1:30842527-30842546 Msa1015930:intron 40.0%
ATGGTACATGGCAGAATGAT+GGG - chr7_1:30842528-30842547 Msa1015930:intron 40.0%
! AGCACTCTTTTCTCCACAAA+TGG - chr7_1:30842630-30842649 Msa1015930:intron 40.0%
AATGCAGGTTTACTAGGAGG+AGG + chr7_1:30840319-30840338 None:intergenic 45.0%
TTCTCCATCAGGATGAATGC+AGG + chr7_1:30840334-30840353 None:intergenic 45.0%
GCACACTAAGGACTCTAACA+AGG - chr7_1:30840438-30840457 Msa1015930:intron 45.0%
! CCAGATTTTGTTCGTGTGCA+TGG + chr7_1:30840897-30840916 None:intergenic 45.0%
CCATGCACACGAACAAAATC+TGG - chr7_1:30840894-30840913 Msa1015930:intron 45.0%
CAACACAACCTTATACGTGG+AGG + chr7_1:30840992-30841011 None:intergenic 45.0%
! GAAAAAGGGTGCAAAGTGGT+TGG - chr7_1:30841136-30841155 Msa1015930:intron 45.0%
CCCATAGTTGGGAAAACACA+GGG + chr7_1:30841272-30841291 None:intergenic 45.0%
! CCCTGTGTTTTCCCAACTAT+GGG - chr7_1:30841269-30841288 Msa1015930:intron 45.0%
TCCCATAGTTGGGAAAACAC+AGG + chr7_1:30841273-30841292 None:intergenic 45.0%
CCAGTGTGTAATCTGTAGTG+GGG - chr7_1:30841805-30841824 Msa1015930:intron 45.0%
! GTAGTGGGGACTTGTATTCT+TGG - chr7_1:30841819-30841838 Msa1015930:intron 45.0%
AAGCATGGGTACTCCAAACA+TGG - chr7_1:30841930-30841949 Msa1015930:intron 45.0%
! CTCCAAACATGGAGAAGTAC+CGG - chr7_1:30841941-30841960 Msa1015930:intron 45.0%
TACCGGTACTTCTCCATGTT+TGG + chr7_1:30841946-30841965 None:intergenic 45.0%
TACTGTACACGTACCCGATA+CGG + chr7_1:30841981-30842000 None:intergenic 45.0%
GTTGGTACCGCAAAAACGTA+CGG - chr7_1:30842053-30842072 Msa1015930:intron 45.0%
TTGGTACCGCAAAAACGTAC+GGG - chr7_1:30842054-30842073 Msa1015930:intron 45.0%
!! TACGGTACCGGTACGTAATA+CGG + chr7_1:30842372-30842391 None:intergenic 45.0%
GCCGGTATTACAGACGTATG+TGG - chr7_1:30840409-30840428 Msa1015930:intron 50.0%
TGCATGGGTTCCAGAAACGA+AGG + chr7_1:30840881-30840900 None:intergenic 50.0%
TTTCCCTGCCATTCCTCTTC+AGG - chr7_1:30841101-30841120 Msa1015930:intron 50.0%
ATCGAGCACCTGAAGAGGAA+TGG + chr7_1:30841112-30841131 None:intergenic 50.0%
CCAATATCGAGCACCTGAAG+AGG + chr7_1:30841117-30841136 None:intergenic 50.0%
CCTCTTCAGGTGCTCGATAT+TGG - chr7_1:30841114-30841133 Msa1015930:intron 50.0%
! GCCCTGTGTTTTCCCAACTA+TGG - chr7_1:30841268-30841287 Msa1015930:intron 50.0%
!! GGGTTTTGGTCAAGGTCTCA+AGG - chr7_1:30841504-30841523 Msa1015930:intron 50.0%
CCCCACTACAGATTACACAC+TGG + chr7_1:30841808-30841827 None:intergenic 50.0%
ACTGTACACGTACCCGATAC+GGG + chr7_1:30841980-30841999 None:intergenic 50.0%
! TCAACGCCCGTACGTTTTTG+CGG + chr7_1:30842063-30842082 None:intergenic 50.0%
! GTATTACGTACCGGTACCGT+AGG - chr7_1:30842371-30842390 Msa1015930:intron 50.0%
TGCCCGTACCTATGCATCAT+AGG - chr7_1:30842399-30842418 Msa1015930:intron 50.0%
GCACCTGAAGAGGAATGGCA+GGG + chr7_1:30841107-30841126 None:intergenic 55.0%
AGCACCTGAAGAGGAATGGC+AGG + chr7_1:30841108-30841127 None:intergenic 55.0%
!! CATGGAGAAGTACCGGTAGC+AGG - chr7_1:30841948-30841967 Msa1015930:intron 55.0%
TAGCAGGTACGCACCCGTAT+CGG - chr7_1:30841964-30841983 Msa1015930:intron 55.0%
AGGTACGGGCACAGTACCTA+CGG + chr7_1:30842390-30842409 None:intergenic 55.0%
ACGCAGATGCACCTGCCTCA+AGG - chr7_1:30841233-30841252 Msa1015930:intron 60.0%
TACGGGTGCGTACCTGCTAC+CGG + chr7_1:30841963-30841982 None:intergenic 60.0%
AGCAGGTACGCACCCGTATC+GGG - chr7_1:30841965-30841984 Msa1015930:intron 60.0%
GGGCACAGTACCTACGGTAC+CGG + chr7_1:30842384-30842403 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 30840271 30842823 30840271 ID=Msa1015930;Name=Msa1015930
chr7_1 mRNA 30840271 30842823 30840271 ID=Msa1015930-mRNA-1;Parent=Msa1015930;Name=Msa1015930-mRNA-1;_AED=0.64;_eAED=0.64;_QI=0|0|0|0.5|1|1|2|0|76
chr7_1 exon 30842671 30842823 30842671 ID=Msa1015930-mRNA-1:exon:6176;Parent=Msa1015930-mRNA-1
chr7_1 exon 30840271 30840348 30840271 ID=Msa1015930-mRNA-1:exon:6175;Parent=Msa1015930-mRNA-1
chr7_1 CDS 30842671 30842823 30842671 ID=Msa1015930-mRNA-1:cds;Parent=Msa1015930-mRNA-1
chr7_1 CDS 30840271 30840348 30840271 ID=Msa1015930-mRNA-1:cds;Parent=Msa1015930-mRNA-1
Gene Sequence

>Msa1015930

ATGGCTACAGTAATTTCTATGCTTAACAGTGAGGTTGCATCACTTCCTCCTCCTAGTAAACCTGCATTCATCCTGATGGAGAATATGTTGAATTCAAAGACACCTGAAGAAAATCGAAGTATATGTTCTATTAATACTGCCGGTATTACAGACGCCCTCCATTTGTTACATGTTATTCTTGAAGATTCCAGAATTTTTATTTCCATCAAAATATCAACTCCACTAATTTGA

Protein sequence

>Msa1015930

MATVISMLNSEVASLPPPSKPAFILMENMLNSKTPEENRSICSINTAGITDALHLLHVILEDSRIFISIKISTPLI*