AlfalfaGEDB | Msa1445320

M. sativa cultivar zhongmu-4 / Msa1445320

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1445320	A0A072TTW9	92.461	955	62	5	1	950	1	950	0.0	1747

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TFs/TRs:

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Gene ID	Type	Classification
Msa1445320	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1301980	Msa1445320	0.810945	8.955553e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1445320	MtrunA17_Chr8g0377811	92.461	955	62	5	1	950	1	950	0.0	1747

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1445320	AT4G14200.1	42.623	122	62	6	829	945	665	783	1.36e-16	85.5
Msa1445320	AT4G14200.1	39.831	118	54	4	1	104	1	115	4.13e-14	77.4

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Find 195 sgRNAs with CRISPR-Local

Find 334 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACAGGGTTGAAATCA+TGG	0.423875	tig0031043:+8423	Msa1445320:CDS
AAAGGTTGCAGGAGCAGATG+TGG	0.573435	tig0031043:+9197	Msa1445320:CDS
AAATGTGACAGATTGTAGTT+TGG	0.486137	tig0031043:+8348	Msa1445320:CDS
AACAATGACAAGGGTAATGA+TGG	0.452738	tig0031043:+6757	Msa1445320:CDS
AACCAGTTGAGTGAAAGAAG+TGG	0.653078	tig0031043:+8838	Msa1445320:CDS
AACTGATGTTTCAGTTGACT+CGG	0.491776	tig0031043:+9830	Msa1445320:CDS
AAGAAAAGGCTGCAAATGAA+TGG	0.500017	tig0031043:+10495	Msa1445320:CDS
AAGATCTGTGGAATCATTGA+AGG	0.513923	tig0031043:+6516	Msa1445320:CDS
AAGGAGGAGTAAGTTGAGAG+TGG	0.535658	tig0031043:-5978	Msa1445320:intergenic
AAGGCCAACGAAAGAAAGAA+AGG	0.506117	tig0031043:-6117	Msa1445320:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATGATGAAATGGAATGGA+AGG	-	tig0031043:6072-6091	Msa1053850:intergenic	30.0%
AGGATAATGATGAAATGGAA+TGG	-	tig0031043:6076-6095	Msa1053850:intergenic	30.0%
AGAAGAGGATAATGATGAAA+TGG	-	tig0031043:6081-6100	Msa1053850:intergenic	30.0%
AGTCAACGAAAGAAAAGAAA+AGG	-	tig0031043:6139-6158	Msa1445320:intergenic	30.0%
GAAGAACAAACCAATTTCAA+TGG	+	tig0031043:6546-6565	Msa1445320:CDS	30.0%
ATCATCTGAACCATTGAAAT+TGG	-	tig0031043:6559-6578	Msa1445320:intergenic	30.0%
ATTGTAACTGTAAACTTACC+TGG	-	tig0031043:6594-6613	Msa1445320:intergenic	30.0%
ATTTAGTAGATCAGAAAGTG+AGG	+	tig0031043:6792-6811	Msa1445320:CDS	30.0%
CAAGAACATAATTTGAACTC+AGG	+	tig0031043:6883-6902	Msa1445320:CDS	30.0%
TTGTACTTGGAAGACATTAA+AGG	-	tig0031043:6913-6932	Msa1445320:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0031043	gene	5951	10893	5951	ID=Msa1445320;Name=Msa1445320
tig0031043	mRNA	5951	10893	5951	ID=Msa1445320-mRNA-1;Parent=Msa1445320;Name=Msa1445320-mRNA-1;_AED=0.02;_eAED=0.02;_QI=227\|1\|1\|1\|1\|1\|5\|262\|950
tig0031043	exon	5951	6214	5951	ID=Msa1445320-mRNA-1:exon:14937;Parent=Msa1445320-mRNA-1
tig0031043	exon	6424	6594	6424	ID=Msa1445320-mRNA-1:exon:14938;Parent=Msa1445320-mRNA-1
tig0031043	exon	6737	6904	6737	ID=Msa1445320-mRNA-1:exon:14939;Parent=Msa1445320-mRNA-1
tig0031043	exon	8062	9897	8062	ID=Msa1445320-mRNA-1:exon:14940;Parent=Msa1445320-mRNA-1
tig0031043	exon	9991	10893	9991	ID=Msa1445320-mRNA-1:exon:14941;Parent=Msa1445320-mRNA-1
tig0031043	five_prime_UTR	5951	6177	5951	ID=Msa1445320-mRNA-1:five_prime_utr;Parent=Msa1445320-mRNA-1
tig0031043	CDS	6178	6214	6178	ID=Msa1445320-mRNA-1:cds;Parent=Msa1445320-mRNA-1
tig0031043	CDS	6424	6594	6424	ID=Msa1445320-mRNA-1:cds;Parent=Msa1445320-mRNA-1
tig0031043	CDS	6737	6904	6737	ID=Msa1445320-mRNA-1:cds;Parent=Msa1445320-mRNA-1
tig0031043	CDS	8062	9897	8062	ID=Msa1445320-mRNA-1:cds;Parent=Msa1445320-mRNA-1
tig0031043	CDS	9991	10631	9991	ID=Msa1445320-mRNA-1:cds;Parent=Msa1445320-mRNA-1
tig0031043	three_prime_UTR	10632	10893	10632	ID=Msa1445320-mRNA-1:three_prime_utr;Parent=Msa1445320-mRNA-1

Gene Sequence

>Msa1445320

ATGCATCACCAAGATCAAACCAAAACTCACACTCCAGGGAACGAGAATCATGGAGTTTATGTGTGTCACAAATGTGGTTGGCCTTTTCCAAATCCACATCCCAGTGCTAAACATAGACGTGCTCACAAGAAGATCTGTGGAATCATTGAAGGTTACAAAGAAGAACAAACCAATTTCAATGGTTCAGATGATGATTACAAAACACCAGGTTTGGTTGAGTTGGGTTCAAACAATGACAAGGGTAATGATGGAATTGAAAGGAAATTTAGTAGATCAGAAAGTGAGGTGTATTCAGATGCTGTTGATGATTTTCCGGATAGTGGACTAAGTCAAGGAGTTAAACAGAATTTGCAACAAGAACATAATTTGAACTCAGCTGCAGATGTGAGTCCACTTATTGCTAGCTCAAGCAATGACTGCCAAATTATAAATCCAAAAATTATGCAAAGTGTAAGTTTTGAAGTGGGAAATATAGGTGGGACGCAAAGTCAATTGCCAGGCTCTACTGTGGATCCATTAACAAGCTCAATTGCAGATTCAAAGAATGAAGAATCGAGCATTGTGCATGGCGATGGTTTTTCTGGTTTGTCAAGTGACTCAAGTCTCAGCATAGCTGAAGCCGTGCCCAATTTATTGCCAGAAAAAAATATTTATGCTGGTGAAAATGTGACAGATTGTAGTTTGGTGTGTGATGAAAAGGAGCTTAATTTGAAAGGAAGCGATGAGGTAAAATCAGAAAAAGACAGGGTTGAAATCATGGAGTCAACTGATAACGTTGTAGGTGAAACATATGAAGGAACTTCTAAAATAGTAGTTAACGAAGCAATTTCCCTGGATCATGACATGGGCGATGGAGCTGTTAATCCGAAGGAAAAGAAGGAACCCGGGTCCCTTTCTTTGCTGCCCCAGTATGAATTTCCTCAAGAAGTAAACTCGCCAATAATCACGAACGAGGCTCAGGTAGAATCTGCGCATGCAATACATTCTACTACTTGTAATGAAGTCGAGGTTTTGCCAGAAAAGGAAGACGTGAATGTTAATATTGATCCACTTCCTGTGCTTGATGATAAACTTGATGCAGCTTATCCTGAGAGCGTGTCATTGAAACATGAGGAACATGTTACTGAGGAAAATAATTTTCATTTCAACACAAACCAGTTGAGTGAAAGAAGTGGTGTCCTCTCTTCAGAGATGCATGTTATGGATAATGACACGAAGACTGAAAATCTACATGCTGAAGATTGCTCTGAAGTTTCTTTGGTTGAGCTCACAACTGAAACTTATCAAATATCGCATGAAATCAGAGTGTCTACGAAAACTGAGATGAATGAGAATAATTTTCCTGAAGAGCATGGACCTGATGAAATTCATGAAAGTTCTCAACCTGAAAGTAGTTTAATGGTCTCAGCAAATGAATTTCAGAGGGAGGCATCCTTTCGGTCTGCGACTGATGAAACATTTAGCATCATCAGTAATGACACAACTGAAATAAACGATGCATCTGTAGTTGGAAAGGTTGCAGGAGCAGATGTGGAAAGTGACACTGAAGTTATAGTGGATTTTCAACCTCGTAGTGACCACTTGCAATCAGAAGTTGAGCAATCAAGTGAGTTATTTAGGAATAATAGTGATGATGCTGGTGAAAATGGCAAAATTGAAGATGAAATCAATAGCAATATCAAGTTATATGAAGAGAATAAGAAACCGACTGGTATTGCTGCTGACTCGCACGAAGAACAGGATGAACAGCTATCAGTGAAGGCTGCTGAAGACTTTTCTCGCAAGCATACATCACATTCTTCGACAAATGCTGAGCTTTCTGCTGAACCGGATTCCGCTGTTGAAGATGATTCAATTGGAGAGCCTGTTCAAGATCAACGCCGTAATAACTTGGTTAAACTTGGTTCATCTGGAATTGATACTTCTGCTGACTCACATGAAGCACGGGATGCACAACTGTTAGTGAAGGCTACAGAAGACCTTGCCAGCAAGTATACATCACATTCTTCTATAAATTCCGGGGCTTCTGCTCGACATGATTCTGCTGTTGAAGATAATTCAGGTGAAGTGTCTAGAGTTACTGCTGTGCTAATGCCGGTTGATGATCAAAGCAATAATAACTTGACTAAATTAACTCCACCCGGAACTGATGTTTCAGTTGACTCGGGTAGCCGGCGTGACAGTTTGGAAGGCAACTGGGGTTCTGGTTCAGTTATCTCGATGATATCCGATGTACCAGCTGTTACTGATGTGGAAACTTTACCATCAACCGGTTCGCTGGCATCGACAGAAGCAGGCAAATCAGACTTGAACGTCCGACAAGCTGCACCTGCTGAGAGACAGCTGTCTGGAAAATCAGAAACATTTGAGCTGCCATCTTTCACAACATTGGTTGAGCCTAGCCACGTGGCTAGTCCTAAAGGCGCCACTTCTGAAACCGTAAAACCACAACAGTCAAATTCCACCTCTAGTTGGTTCCCAACTTTAAATCAGGTTATCAATGAGTCTGAAGCGAAAAAGAAGAATGAAGAAAATATCGCCAAGATAACAAACCAGAGCAGAAGTAAAGAGCACACACCTCTCAAGAGTCTTTTGGGTGAGGCTACTCCTAGCAACAAGCCGAAATCACCAAAAATAGAAGAAAATAATGGTTCTGGATTGACAACTGTTAACTCAATTCTTGGTCCTGAGTCCCCTTCAGCGACTCAAGTGGTGAAAGAAAAGGCTGCAAATGAATGGAACTCTCCAGCAAGATATCCTGCAAATATCAAGAGGGAAAAAAAGAAATTGAAGAGCAGACCATTTTGGATACAGTTGGTTTGTTGCACAACAGTGGATCCTCAGCGAAGGTAG

Protein sequence

>Msa1445320

MHHQDQTKTHTPGNENHGVYVCHKCGWPFPNPHPSAKHRRAHKKICGIIEGYKEEQTNFNGSDDDYKTPGLVELGSNNDKGNDGIERKFSRSESEVYSDAVDDFPDSGLSQGVKQNLQQEHNLNSAADVSPLIASSSNDCQIINPKIMQSVSFEVGNIGGTQSQLPGSTVDPLTSSIADSKNEESSIVHGDGFSGLSSDSSLSIAEAVPNLLPEKNIYAGENVTDCSLVCDEKELNLKGSDEVKSEKDRVEIMESTDNVVGETYEGTSKIVVNEAISLDHDMGDGAVNPKEKKEPGSLSLLPQYEFPQEVNSPIITNEAQVESAHAIHSTTCNEVEVLPEKEDVNVNIDPLPVLDDKLDAAYPESVSLKHEEHVTEENNFHFNTNQLSERSGVLSSEMHVMDNDTKTENLHAEDCSEVSLVELTTETYQISHEIRVSTKTEMNENNFPEEHGPDEIHESSQPESSLMVSANEFQREASFRSATDETFSIISNDTTEINDASVVGKVAGADVESDTEVIVDFQPRSDHLQSEVEQSSELFRNNSDDAGENGKIEDEINSNIKLYEENKKPTGIAADSHEEQDEQLSVKAAEDFSRKHTSHSSTNAELSAEPDSAVEDDSIGEPVQDQRRNNLVKLGSSGIDTSADSHEARDAQLLVKATEDLASKYTSHSSINSGASARHDSAVEDNSGEVSRVTAVLMPVDDQSNNNLTKLTPPGTDVSVDSGSRRDSLEGNWGSGSVISMISDVPAVTDVETLPSTGSLASTEAGKSDLNVRQAAPAERQLSGKSETFELPSFTTLVEPSHVASPKGATSETVKPQQSNSTSSWFPTLNQVINESEAKKKNEENIAKITNQSRSKEHTPLKSLLGEATPSNKPKSPKIEENNGSGLTTVNSILGPESPSATQVVKEKAANEWNSPARYPANIKREKKKLKSRPFWIQLVCCTTVDPQRR*