AlfalfaGEDB | Msa1448930

M. sativa cultivar zhongmu-4 / Msa1448930

KEGG: K09422
GO: GO:0000003 GO:0000902 GO:0000904 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010026 GO:0010090 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032989 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044249 GO:0044424 GO:0044464 GO:0048316 GO:0048354 GO:0048359 GO:0048468 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090626 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1448930	sp\|A0A4D6Q4S0\|MYB2_CROXC	57.377	183	68	3	1	180	1	176	1.26e-64	205

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1448930	A0A072U6A5	87.209	258	22	3	1	250	1	255	1.27e-160	454

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TFs/TRs:

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Gene ID	Type	Classification
Msa1448930	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0580960	Msa1448930	0.809326	1.999690e-50	-8.615850e-47
Msa1408130	Msa1448930	0.817459	3.261209e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1448930	MtrunA17_Chr7g0275911	87.209	258	22	3	1	250	1	255	2.45e-164	454
Msa1448930	MtrunA17_Chr8g0346561	52.273	308	76	11	1	250	1	295	2.16e-95	281
Msa1448930	MtrunA17_Chr4g0070611	50.220	227	91	4	1	220	1	212	1.87e-69	216
Msa1448930	MtrunA17_Chr4g0070641	60.000	170	56	2	1	163	1	165	1.02e-67	206
Msa1448930	MtrunA17_Chr3g0121241	79.279	111	23	0	5	115	35	145	4.21e-65	204
Msa1448930	MtrunA17_Chr4g0068061	72.174	115	32	0	1	115	1	115	4.68e-63	200
Msa1448930	MtrunA17_Chr4g0037141	49.505	202	100	2	1	200	1	202	1.09e-62	197
Msa1448930	MtrunA17_Chr1g0194951	73.043	115	31	0	1	115	1	115	2.49e-61	197
Msa1448930	MtrunA17_Chr1g0187761	60.690	145	44	1	1	132	1	145	1.23e-60	191
Msa1448930	MtrunA17_Chr3g0131121	72.174	115	32	0	1	115	1	115	1.45e-59	190

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1448930	AT5G49330.1	74.783	115	29	0	1	115	1	115	5.84e-63	201
Msa1448930	AT3G13540.1	48.095	210	87	4	4	207	15	208	9.58e-63	197
Msa1448930	AT4G38620.1	55.747	174	60	3	1	173	1	158	9.81e-63	198
Msa1448930	AT2G47460.1	59.119	159	52	2	1	155	1	150	1.94e-62	200
Msa1448930	AT4G09460.1	47.500	200	92	2	1	194	1	193	4.30e-62	195
Msa1448930	AT1G22640.1	66.667	129	43	0	1	129	1	129	5.23e-62	195
Msa1448930	AT1G06180.1	62.222	135	51	0	1	135	1	135	1.04e-61	194
Msa1448930	AT2G16720.1	70.435	115	34	0	1	115	1	115	2.82e-60	191
Msa1448930	AT3G62610.1	72.174	115	32	0	1	115	1	115	1.87e-59	191
Msa1448930	AT3G23250.1	66.667	120	40	0	1	120	1	120	1.90e-59	190

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Find 56 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCTTCTTCACCCTTAC+AGG	0.210306	tig0032353:+855	None:intergenic
AACAACAAGCCAAGCGGAAG+AGG	0.365593	tig0032353:-778	Msa1448930:CDS
AACTGATAGTACCTTGAACT+TGG	0.466248	tig0032353:-814	Msa1448930:CDS
AAGCTCATGGAGAAGGCCAA+TGG	0.567347	tig0032353:-1557	Msa1448930:CDS
AAGGAGAACAAAGTTGTATA+TGG	0.265595	tig0032353:-1746	Msa1448930:five_prime_UTR
AATAGATGGTCCCTTATTGC+GGG	0.508874	tig0032353:-1038	Msa1448930:CDS
ACAAAAGAGAGAGACAATGA+TGG	0.536178	tig0032353:-738	Msa1448930:CDS
ACAACAAGCCAAGCGGAAGA+GGG	0.532451	tig0032353:-777	Msa1448930:CDS
ACCAAATATATTCAAGCTCA+TGG	0.438518	tig0032353:-1570	Msa1448930:CDS
ACTCCTTGTTGTTCAAAAGT+TGG	0.384251	tig0032353:-1636	Msa1448930:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGGATAATGACATGATGA+TGG	+	tig0032353:578-597	None:intergenic	30.0%
ATAGCTTATACAAGCTCTTA+AGG	+	tig0032353:596-615	None:intergenic	30.0%
TTGGAGTTTGGACATTTAAA+AGG	-	tig0032353:619-638	Msa1448930:CDS	30.0%
AAGGAGAACAAAGTTGTATA+TGG	-	tig0032353:638-657	Msa1448930:CDS	30.0%
AGGAGAACAAAGTTGTATAT+GGG	-	tig0032353:639-658	Msa1448930:CDS	30.0%
ACCAAATATATTCAAGCTCA+TGG	-	tig0032353:814-833	Msa1448930:CDS	30.0%
TCCATGAGCTTGAATATATT+TGG	+	tig0032353:818-837	None:intergenic	30.0%
ATGTTTCCTCTCTTTATATC+TGG	+	tig0032353:1277-1296	None:intergenic	30.0%
ATGAGAATGCATTCACTTAT+TGG	-	tig0032353:1322-1341	Msa1448930:intron	30.0%
TAAGGATAATGACATGATGA+TGG	+	tig0032353:578-597	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0032353	gene	569	1837	569	ID=Msa1448930;Name=Msa1448930
tig0032353	mRNA	569	1837	569	ID=Msa1448930-mRNA-1;Parent=Msa1448930;Name=Msa1448930-mRNA-1;_AED=0.05;_eAED=0.05;_QI=170\|1\|0.5\|1\|1\|1\|2\|0\|250
tig0032353	exon	569	1185	569	ID=Msa1448930-mRNA-1:exon:16726;Parent=Msa1448930-mRNA-1
tig0032353	exon	1535	1837	1535	ID=Msa1448930-mRNA-1:exon:16725;Parent=Msa1448930-mRNA-1
tig0032353	five_prime_UTR	1668	1837	1668	ID=Msa1448930-mRNA-1:five_prime_utr;Parent=Msa1448930-mRNA-1
tig0032353	CDS	1535	1667	1535	ID=Msa1448930-mRNA-1:cds;Parent=Msa1448930-mRNA-1
tig0032353	CDS	569	1185	569	ID=Msa1448930-mRNA-1:cds;Parent=Msa1448930-mRNA-1

Gene Sequence

>Msa1448930

ATGGGAAGAACTCCTTGTTGTTCAAAAGTTGGGTTGCATAGAGGTCCATGGACTTCACGTGAAGATGCATTGCTCACCAAATATATTCAAGCTCATGGAGAAGGCCAATGGAGATCACTTCCAAAAAGAGCTGGGCTTCTTAGATGTGGAAAAAGTTGCAGACTAAGATGGATGAACTATCTAAGACCAGATATAAAGAGAGGAAACATAACTCCAGAAGAAGATGACCTTATCATGAGAATGCATTCACTTATTGGTAATAGATGGTCCCTTATTGCGGGAAGATTACCAGGACGTACAGATAATGAGATAAAGAATTACTGGAACACACATCTCAGTAAAAAGAAGAAAAAGAAGCAGAAGAAAAATGACAAGAACAGAGTCATAATAACCAAAGTACCAGAAGATTCAGAGAAGAAGACCTTAGTTTATCTACCAAAGCCTGTAAGGGTGAAGAAGACTTTATCATCAATAATACCAAGAACTGATAGTACCTTGAACTTGGAATACAACTCAGCAACAACAAGCCAAGCGGAAGAGGGAGAGCAAAGAGCAGAAACAAAAGAGAGAGACAATGATGGGTTTGGATTGTTGTTTGAGGAACAGTACCATGCAGACTTGGTCAACAATGATGACATGGAATGCCAATCTGGTTTTCTTGAGAAGATGTATGAAGAGTACTTGCAGCTATTGAAGAATGAAGAAAATCATGAAAAAATACTAGATTCTTCTTTTGCGGAGTCCTTATTA

Protein sequence

>Msa1448930

MGRTPCCSKVGLHRGPWTSREDALLTKYIQAHGEGQWRSLPKRAGLLRCGKSCRLRWMNYLRPDIKRGNITPEEDDLIMRMHSLIGNRWSLIAGRLPGRTDNEIKNYWNTHLSKKKKKKQKKNDKNRVIITKVPEDSEKKTLVYLPKPVRVKKTLSSIIPRTDSTLNLEYNSATTSQAEEGEQRAETKERDNDGFGLLFEEQYHADLVNNDDMECQSGFLEKMYEEYLQLLKNEENHEKILDSSFAESLL