Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449300 | sp|Q9SKC9|NDUB7_ARATH | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 2.55e-49 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449300 | A0A072VQV0 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 3.32e-67 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1428810 | Msa1449300 | 0.839963 | 1.156683e-57 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449300 | MtrunA17_Chr1g0210951 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 6.40e-71 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449300 | AT2G02050.1 | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 2.59e-50 | 154 |
Find 25 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCAAGCATTGGCGGGTT+TGG | 0.229519 | tig0032509:+12598 | None:intergenic |
| GATCAAGCATTGGCGGGTTT+GGG | 0.236660 | tig0032509:+12599 | None:intergenic |
| ACCCTCTGAAACGGGTTGTT+TGG | 0.368427 | tig0032509:+12640 | None:intergenic |
| CGTTCGTTCTCGCACTTCCA+TGG | 0.425940 | tig0032509:+12749 | None:intergenic |
| AAGAGGAGATGGTGGAAGCT+AGG | 0.454716 | tig0032509:-12850 | Msa1449300:CDS |
| ATAGCAGCACCCTCTGAAAC+GGG | 0.480710 | tig0032509:+12632 | None:intergenic |
| AATAGCAGCACCCTCTGAAA+CGG | 0.485923 | tig0032509:+12631 | None:intergenic |
| CTTGTCTGCATTTGTTGAGA+GGG | 0.492622 | tig0032509:+12786 | None:intergenic |
| GGTGGAAGCTAGGGTTCCAT+TGG | 0.505790 | tig0032509:-12840 | Msa1449300:CDS |
| GCTTGTCTGCATTTGTTGAG+AGG | 0.517488 | tig0032509:+12785 | None:intergenic |
| AAGCTGAATTCTATCTTCCA+TGG | 0.530156 | tig0032509:-12766 | Msa1449300:CDS |
| AGAGGAGATGGTGGAAGCTA+GGG | 0.534962 | tig0032509:-12849 | Msa1449300:CDS |
| ATCAAGCATTGGCGGGTTTG+GGG | 0.544039 | tig0032509:+12600 | None:intergenic |
| GAGAGGGATCAAGCATTGGC+GGG | 0.545254 | tig0032509:+12593 | None:intergenic |
| GTGCGAATACGAACTCGTCA+TGG | 0.547428 | tig0032509:-12711 | Msa1449300:CDS |
| AGAGAGGGATCAAGCATTGG+CGG | 0.549265 | tig0032509:+12592 | None:intergenic |
| TCCAAACAACCCGTTTCAGA+GGG | 0.555884 | tig0032509:-12641 | Msa1449300:CDS |
| GAATCGAAGATGGAATTTGA+AGG | 0.557443 | tig0032509:-12899 | None:intergenic |
| GAGAGAGAGGGATCAAGCAT+TGG | 0.561121 | tig0032509:+12589 | None:intergenic |
| TTCCAAACAACCCGTTTCAG+AGG | 0.569899 | tig0032509:-12642 | Msa1449300:CDS |
| TTGGCGGGTTTGGGGATGAG+AGG | 0.579099 | tig0032509:+12608 | None:intergenic |
| GATTGCGACACAAGAGGAGA+TGG | 0.580463 | tig0032509:-12861 | Msa1449300:CDS |
| CATTGATCTCTGTAAGCCAA+TGG | 0.586159 | tig0032509:+12824 | None:intergenic |
| TGCGACACAAGAGGAGATGG+TGG | 0.590865 | tig0032509:-12858 | Msa1449300:CDS |
| GAAGATGATTGCGACACAAG+AGG | 0.593830 | tig0032509:-12867 | Msa1449300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAGCATTTTGATTTTCG+CGG | + | tig0032509:12831-12850 | None:intergenic | 25.0% |
| AAGCTGAATTCTATCTTCCA+TGG | - | tig0032509:12725-12744 | Msa1449300:CDS | 35.0% | |
| CATTGATCTCTGTAAGCCAA+TGG | + | tig0032509:12670-12689 | None:intergenic | 40.0% | |
| ! | CTTGTCTGCATTTGTTGAGA+GGG | + | tig0032509:12708-12727 | None:intergenic | 40.0% |
| GAAGATGATTGCGACACAAG+AGG | - | tig0032509:12624-12643 | Msa1449300:CDS | 45.0% | |
| ! | GCTTGTCTGCATTTGTTGAG+AGG | + | tig0032509:12709-12728 | None:intergenic | 45.0% |
| TTCCAAACAACCCGTTTCAG+AGG | - | tig0032509:12849-12868 | Msa1449300:CDS | 45.0% | |
| TCCAAACAACCCGTTTCAGA+GGG | - | tig0032509:12850-12869 | Msa1449300:CDS | 45.0% | |
| ! | AATAGCAGCACCCTCTGAAA+CGG | + | tig0032509:12863-12882 | None:intergenic | 45.0% |
| GATTGCGACACAAGAGGAGA+TGG | - | tig0032509:12630-12649 | Msa1449300:CDS | 50.0% | |
| !! | AAGAGGAGATGGTGGAAGCT+AGG | - | tig0032509:12641-12660 | Msa1449300:CDS | 50.0% |
| !! | AGAGGAGATGGTGGAAGCTA+GGG | - | tig0032509:12642-12661 | Msa1449300:CDS | 50.0% |
| GTGCGAATACGAACTCGTCA+TGG | - | tig0032509:12780-12799 | Msa1449300:CDS | 50.0% | |
| !! | ACCCTCTGAAACGGGTTGTT+TGG | + | tig0032509:12854-12873 | None:intergenic | 50.0% |
| !! | ATAGCAGCACCCTCTGAAAC+GGG | + | tig0032509:12862-12881 | None:intergenic | 50.0% |
| TGCGACACAAGAGGAGATGG+TGG | - | tig0032509:12633-12652 | Msa1449300:CDS | 55.0% | |
| GGTGGAAGCTAGGGTTCCAT+TGG | - | tig0032509:12651-12670 | Msa1449300:CDS | 55.0% | |
| CGTTCGTTCTCGCACTTCCA+TGG | + | tig0032509:12745-12764 | None:intergenic | 55.0% | |
| !! | TTGGCGGGTTTGGGGATGAG+AGG | + | tig0032509:12886-12905 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0032509 | gene | 12601 | 12912 | 12601 | ID=Msa1449300;Name=Msa1449300 |
| tig0032509 | mRNA | 12601 | 12912 | 12601 | ID=Msa1449300-mRNA-1;Parent=Msa1449300;Name=Msa1449300-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|103 |
| tig0032509 | exon | 12601 | 12912 | 12601 | ID=Msa1449300-mRNA-1:exon:16902;Parent=Msa1449300-mRNA-1 |
| tig0032509 | CDS | 12601 | 12912 | 12601 | ID=Msa1449300-mRNA-1:cds;Parent=Msa1449300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
