Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1428810 | XP_013470311.1 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 6.96e-67 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1428810 | sp|Q9SKC9|NDUB7_ARATH | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 2.55e-49 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1428810 | A0A072VQV0 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 3.32e-67 | 206 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0045730 | Msa1428810 | 0.871481 | 6.612584e-67 | -8.615850e-47 |
| Msa0052300 | Msa1428810 | -0.804517 | 2.079605e-49 | -8.615850e-47 |
| Msa0116130 | Msa1428810 | 0.823036 | 1.719057e-53 | -8.615850e-47 |
| Msa1362790 | Msa1428810 | 0.804040 | 2.614474e-49 | -8.615850e-47 |
| Msa1369190 | Msa1428810 | 0.830708 | 2.522800e-55 | -8.615850e-47 |
| Msa1410440 | Msa1428810 | 0.816422 | 5.577056e-52 | -8.615850e-47 |
| Msa1413180 | Msa1428810 | 0.825755 | 3.941988e-54 | -8.615850e-47 |
| Msa1428810 | Msa1429500 | 0.840798 | 6.997209e-58 | -8.615850e-47 |
| Msa1428810 | Msa1442850 | 0.827703 | 1.351474e-54 | -8.615850e-47 |
| Msa1428810 | Msa1449300 | 0.839963 | 1.156683e-57 | -8.615850e-47 |
| Msa1428810 | Msa1457400 | 0.804243 | 2.372417e-49 | -8.615850e-47 |
| Msa1428810 | Msa1462860 | 0.843029 | 1.801358e-58 | -8.615850e-47 |
| Msa0604960 | Msa1428810 | 0.822938 | 1.812138e-53 | -8.615850e-47 |
| Msa0619860 | Msa1428810 | 0.803753 | 2.999522e-49 | -8.615850e-47 |
| Msa0622750 | Msa1428810 | 0.849195 | 3.778458e-60 | -8.615850e-47 |
| Msa0623200 | Msa1428810 | 0.873829 | 1.085673e-67 | -8.615850e-47 |
| Msa0659030 | Msa1428810 | 0.834708 | 2.564262e-56 | -8.615850e-47 |
| Msa0683780 | Msa1428810 | 0.826807 | 2.214994e-54 | -8.615850e-47 |
| Msa0704660 | Msa1428810 | 0.819388 | 1.192548e-52 | -8.615850e-47 |
| Msa0705000 | Msa1428810 | 0.832757 | 7.879771e-56 | -8.615850e-47 |
| Msa0705040 | Msa1428810 | 0.827763 | 1.307208e-54 | -8.615850e-47 |
| Msa0706500 | Msa1428810 | 0.835414 | 1.701434e-56 | -8.615850e-47 |
| Msa0712610 | Msa1428810 | 0.800609 | 1.330024e-48 | -8.615850e-47 |
| Msa0785130 | Msa1428810 | 0.805818 | 1.110829e-49 | -8.615850e-47 |
| Msa0808800 | Msa1428810 | 0.804401 | 2.198546e-49 | -8.615850e-47 |
| Msa0123010 | Msa1428810 | 0.800306 | 1.533553e-48 | -8.615850e-47 |
| Msa0224560 | Msa1428810 | 0.834465 | 2.951588e-56 | -8.615850e-47 |
| Msa1225660 | Msa1428810 | 0.833272 | 5.866696e-56 | -8.615850e-47 |
| Msa1256950 | Msa1428810 | 0.840428 | 8.747381e-58 | -8.615850e-47 |
| Msa1266060 | Msa1428810 | 0.805003 | 1.646109e-49 | -8.615850e-47 |
| Msa1329510 | Msa1428810 | 0.824252 | 8.928623e-54 | -8.615850e-47 |
| Msa1329540 | Msa1428810 | 0.848269 | 6.823543e-60 | -8.615850e-47 |
| Msa0376820 | Msa1428810 | 0.830322 | 3.134536e-55 | -8.615850e-47 |
| Msa0408240 | Msa1428810 | -0.814958 | 1.181700e-51 | -8.615850e-47 |
| Msa0423750 | Msa1428810 | 0.838696 | 2.466330e-57 | -8.615850e-47 |
| Msa0443390 | Msa1428810 | 0.805881 | 1.077490e-49 | -8.615850e-47 |
| Msa0472130 | Msa1428810 | 0.833687 | 4.620626e-56 | -8.615850e-47 |
| Msa1042780 | Msa1428810 | 0.801057 | 1.077441e-48 | -8.615850e-47 |
| Msa1085470 | Msa1428810 | 0.824372 | 8.364665e-54 | -8.615850e-47 |
| Msa0348830 | Msa1428810 | 0.837847 | 4.081097e-57 | -8.615850e-47 |
| Msa1125500 | Msa1428810 | 0.832157 | 1.109195e-55 | -8.615850e-47 |
| Msa1125580 | Msa1428810 | 0.819118 | 1.373597e-52 | -8.615850e-47 |
| Msa1131170 | Msa1428810 | 0.804182 | 2.442168e-49 | -8.615850e-47 |
| Msa1166870 | Msa1428810 | 0.810857 | 9.356546e-51 | -8.615850e-47 |
| Msa1206090 | Msa1428810 | 0.837608 | 4.701186e-57 | -8.615850e-47 |
| Msa1208020 | Msa1428810 | 0.808342 | 3.245967e-50 | -8.615850e-47 |
| Msa0483110 | Msa1428810 | 0.835711 | 1.430935e-56 | -8.615850e-47 |
| Msa0534750 | Msa1428810 | 0.818454 | 1.943517e-52 | -8.615850e-47 |
| Msa0867060 | Msa1428810 | 0.808013 | 3.814407e-50 | -8.615850e-47 |
| Msa0945480 | Msa1428810 | 0.805262 | 1.453533e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1428810 | MtrunA17_Chr1g0210951 | 96.190 | 105 | 2 | 1 | 1 | 103 | 1 | 105 | 6.40e-71 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1428810 | AT2G02050.1 | 71.845 | 103 | 29 | 0 | 1 | 103 | 1 | 103 | 2.59e-50 | 154 |
Find 25 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATCAAGCATTGGCGGGTT+TGG | 0.229519 | tig0026414:-11757 | None:intergenic |
| GATCAAGCATTGGCGGGTTT+GGG | 0.236660 | tig0026414:-11756 | None:intergenic |
| ACCCTCTGAAACGGGTTGTT+TGG | 0.368427 | tig0026414:-11715 | None:intergenic |
| CGTTCGTTCTCGCACTTCCA+TGG | 0.425940 | tig0026414:-11606 | None:intergenic |
| AAGAGGAGATGGTGGAAGCT+AGG | 0.454716 | tig0026414:+11505 | Msa1428810:CDS |
| ATAGCAGCACCCTCTGAAAC+GGG | 0.480710 | tig0026414:-11723 | None:intergenic |
| AATAGCAGCACCCTCTGAAA+CGG | 0.485923 | tig0026414:-11724 | None:intergenic |
| CTTGTCTGCATTTGTTGAGA+GGG | 0.492622 | tig0026414:-11569 | None:intergenic |
| GGTGGAAGCTAGGGTTCCAT+TGG | 0.505790 | tig0026414:+11515 | Msa1428810:CDS |
| GCTTGTCTGCATTTGTTGAG+AGG | 0.517488 | tig0026414:-11570 | None:intergenic |
| AAGCTGAATTCTATCTTCCA+TGG | 0.530156 | tig0026414:+11589 | Msa1428810:CDS |
| AGAGGAGATGGTGGAAGCTA+GGG | 0.534962 | tig0026414:+11506 | Msa1428810:CDS |
| ATCAAGCATTGGCGGGTTTG+GGG | 0.544039 | tig0026414:-11755 | None:intergenic |
| GAGAGGGATCAAGCATTGGC+GGG | 0.545254 | tig0026414:-11762 | None:intergenic |
| GTGCGAATACGAACTCGTCA+TGG | 0.547428 | tig0026414:+11644 | Msa1428810:CDS |
| AGAGAGGGATCAAGCATTGG+CGG | 0.549265 | tig0026414:-11763 | None:intergenic |
| TCCAAACAACCCGTTTCAGA+GGG | 0.555884 | tig0026414:+11714 | Msa1428810:CDS |
| GAATCGAAGATGGAATTTGA+AGG | 0.557443 | tig0026414:+11456 | None:intergenic |
| GAGAGAGAGGGATCAAGCAT+TGG | 0.561121 | tig0026414:-11766 | None:intergenic |
| TTCCAAACAACCCGTTTCAG+AGG | 0.569899 | tig0026414:+11713 | Msa1428810:CDS |
| TTGGCGGGTTTGGGGATGAG+AGG | 0.579099 | tig0026414:-11747 | None:intergenic |
| GATTGCGACACAAGAGGAGA+TGG | 0.580463 | tig0026414:+11494 | Msa1428810:CDS |
| CATTGATCTCTGTAAGCCAA+TGG | 0.586159 | tig0026414:-11531 | None:intergenic |
| TGCGACACAAGAGGAGATGG+TGG | 0.590865 | tig0026414:+11497 | Msa1428810:CDS |
| GAAGATGATTGCGACACAAG+AGG | 0.593830 | tig0026414:+11488 | Msa1428810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAGCATTTTGATTTTCG+CGG | - | tig0026414:11695-11714 | None:intergenic | 25.0% |
| AAGCTGAATTCTATCTTCCA+TGG | + | tig0026414:11589-11608 | Msa1428810:CDS | 35.0% | |
| CATTGATCTCTGTAAGCCAA+TGG | - | tig0026414:11534-11553 | None:intergenic | 40.0% | |
| ! | CTTGTCTGCATTTGTTGAGA+GGG | - | tig0026414:11572-11591 | None:intergenic | 40.0% |
| GAAGATGATTGCGACACAAG+AGG | + | tig0026414:11488-11507 | Msa1428810:CDS | 45.0% | |
| ! | GCTTGTCTGCATTTGTTGAG+AGG | - | tig0026414:11573-11592 | None:intergenic | 45.0% |
| TTCCAAACAACCCGTTTCAG+AGG | + | tig0026414:11713-11732 | Msa1428810:CDS | 45.0% | |
| TCCAAACAACCCGTTTCAGA+GGG | + | tig0026414:11714-11733 | Msa1428810:CDS | 45.0% | |
| ! | AATAGCAGCACCCTCTGAAA+CGG | - | tig0026414:11727-11746 | None:intergenic | 45.0% |
| GATTGCGACACAAGAGGAGA+TGG | + | tig0026414:11494-11513 | Msa1428810:CDS | 50.0% | |
| !! | AAGAGGAGATGGTGGAAGCT+AGG | + | tig0026414:11505-11524 | Msa1428810:CDS | 50.0% |
| !! | AGAGGAGATGGTGGAAGCTA+GGG | + | tig0026414:11506-11525 | Msa1428810:CDS | 50.0% |
| GTGCGAATACGAACTCGTCA+TGG | + | tig0026414:11644-11663 | Msa1428810:CDS | 50.0% | |
| !! | ACCCTCTGAAACGGGTTGTT+TGG | - | tig0026414:11718-11737 | None:intergenic | 50.0% |
| !! | ATAGCAGCACCCTCTGAAAC+GGG | - | tig0026414:11726-11745 | None:intergenic | 50.0% |
| TGCGACACAAGAGGAGATGG+TGG | + | tig0026414:11497-11516 | Msa1428810:CDS | 55.0% | |
| GGTGGAAGCTAGGGTTCCAT+TGG | + | tig0026414:11515-11534 | Msa1428810:CDS | 55.0% | |
| CGTTCGTTCTCGCACTTCCA+TGG | - | tig0026414:11609-11628 | None:intergenic | 55.0% | |
| !! | TTGGCGGGTTTGGGGATGAG+AGG | - | tig0026414:11750-11769 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0026414 | gene | 11465 | 11776 | 11465 | ID=Msa1428810;Name=Msa1428810 |
| tig0026414 | mRNA | 11465 | 11776 | 11465 | ID=Msa1428810-mRNA-1;Parent=Msa1428810;Name=Msa1428810-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|103 |
| tig0026414 | exon | 11465 | 11776 | 11465 | ID=Msa1428810-mRNA-1:exon:6195;Parent=Msa1428810-mRNA-1 |
| tig0026414 | CDS | 11465 | 11776 | 11465 | ID=Msa1428810-mRNA-1:cds;Parent=Msa1428810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
