AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1450410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450410 MtrunA17_Chr3g0125281 97.619 210 5 0 1 210 1 210 3.53e-141 412
Msa1450410 MtrunA17_Chr1g0159711 65.072 209 54 4 4 210 3 194 2.72e-77 245
Msa1450410 MtrunA17_Chr3g0130431 64.706 153 50 1 9 161 7 155 5.11e-57 191
Msa1450410 MtrunA17_Chr4g0036981 42.759 145 68 3 13 157 6 135 3.83e-27 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450410 AT1G77280.2 61.307 199 68 2 7 205 13 202 3.88e-73 236
Msa1450410 AT1G77280.1 61.307 199 68 2 7 205 13 202 3.88e-73 236
Msa1450410 AT1G21590.1 56.923 195 74 3 11 205 10 194 7.75e-53 181
Msa1450410 AT5G63940.1 55.944 143 59 1 15 157 22 160 3.80e-46 162
Msa1450410 AT1G21590.2 52.288 153 64 2 53 205 11 154 5.73e-32 122
Msa1450410 AT2G16750.2 37.063 143 79 3 15 157 14 145 9.11e-18 81.6
Msa1450410 AT2G16750.1 37.063 143 79 3 15 157 14 145 9.11e-18 81.6

Find 200 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTTCTTGAGCTTCTTTC+AGG 0.101872 tig0032904:+13353 Msa1450410:three_prime_UTR
TTTATTCAGCTCTCTGATTT+TGG 0.163725 tig0032904:+13078 Msa1450410:intron
AAAATTGTTGTCATATTATT+AGG 0.179157 tig0032904:-14634 Msa1450410:intergenic
GAAATATACTGGGCATAAAA+TGG 0.188157 tig0032904:+14804 Msa1450410:three_prime_UTR
CTCATTGGTGGCCTAGCTTT+TGG 0.195272 tig0032904:-13631 Msa1450410:intergenic
AAGGCTCTTCTGCAGATTTA+TGG 0.195874 tig0032904:-11910 Msa1450410:intergenic
CAATGAATTTAACGGAATTA+AGG 0.209624 tig0032904:+14712 Msa1450410:three_prime_UTR
CAACACAAAGCTTCCCTTAT+AGG 0.210924 tig0032904:-14858 Msa1450410:intergenic
TCCTCCTTACCTTAATAATT+AGG 0.217638 tig0032904:+14474 Msa1450410:three_prime_UTR
ATTCCAGTAGATTCGGAAAT+AGG 0.220017 tig0032904:+12240 Msa1450410:three_prime_UTR
ATTCAAGTACCACATTATTT+AGG 0.224249 tig0032904:-13754 Msa1450410:intergenic
GTTGTTGCTCTGCATGTTCT+TGG 0.236521 tig0032904:+10918 Msa1450410:CDS
GCTTAGTTTCCTAATTATTA+AGG 0.243988 tig0032904:-14483 Msa1450410:intergenic
ACAAGATTCTGTTGACCTTT+TGG 0.244001 tig0032904:-13401 Msa1450410:intergenic
GGTGGTGGTGGGAGTGAAAA+TGG 0.247006 tig0032904:+10833 Msa1450410:CDS
ATGAAGTCCCTCCAATTCTT+TGG 0.267865 tig0032904:-12302 Msa1450410:intergenic
AAACATCATATGAAGGCTTT+AGG 0.280846 tig0032904:-12568 Msa1450410:intergenic
TTTGGATAATCAACACTTAT+GGG 0.289473 tig0032904:-13383 Msa1450410:intergenic
ATCATCGGTGAAGAAAATTT+TGG 0.289759 tig0032904:+11360 Msa1450410:CDS
CATGAAGATGCCAAAGCTTA+TGG 0.292080 tig0032904:-14402 Msa1450410:intergenic
CGCCTGTTTATGCAGTTATA+TGG 0.304137 tig0032904:+13268 Msa1450410:intron
AGAAAATAAAAGTTATACTA+TGG 0.320473 tig0032904:+14904 Msa1450410:three_prime_UTR
ACAGATGTTGCTGGAACCTT+TGG 0.323071 tig0032904:+13144 Msa1450410:three_prime_UTR
CATAAGCTTTGGCATCTTCA+TGG 0.335010 tig0032904:+14403 Msa1450410:three_prime_UTR
GGTACTAGCCCAACAAATTA+AGG 0.338121 tig0032904:-14508 Msa1450410:intergenic
CGGTGGCCGGACGGTGGTGG+TGG 0.344552 tig0032904:+10821 Msa1450410:CDS
GGAATTAAGGTATGTGTATC+AGG 0.352412 tig0032904:+14725 Msa1450410:three_prime_UTR
TGGTTGGACACAGAGATATA+AGG 0.357289 tig0032904:+12857 Msa1450410:three_prime_UTR
TTGCCATACATGAAGTATTC+GGG 0.357653 tig0032904:-13293 Msa1450410:intergenic
GTTATTGTTGGAAGTGTTCA+TGG 0.361388 tig0032904:+11415 Msa1450410:CDS
CTGCATGTTCTTGGGACTAA+TGG 0.364560 tig0032904:+10927 Msa1450410:CDS
TACCGTGAATGTTCTCTTCA+AGG 0.365991 tig0032904:-12718 Msa1450410:intergenic
CTAAGCCTCCTTAATTTGTT+GGG 0.372669 tig0032904:+14500 Msa1450410:three_prime_UTR
TTGATTGACAATGAATTTAA+CGG 0.376025 tig0032904:+14704 Msa1450410:three_prime_UTR
TTTGCATCTTCCATAAGCTT+TGG 0.379964 tig0032904:+14392 Msa1450410:three_prime_UTR
AATCCGTCGGGTTCCTCATC+AGG 0.381699 tig0032904:-11794 Msa1450410:intergenic
TCTGCAACTCATGTTATTGT+TGG 0.383290 tig0032904:+11403 Msa1450410:CDS
TTGTTGCTCTGCATGTTCTT+GGG 0.385282 tig0032904:+10919 Msa1450410:CDS
TGTGTTCTTGCTGTGAATAA+TGG 0.385771 tig0032904:+11505 Msa1450410:CDS
TGAGCAAAGTGTTTCATTAG+AGG 0.386128 tig0032904:+14241 Msa1450410:three_prime_UTR
AATTATGTAATAATGCAATA+TGG 0.391565 tig0032904:+14988 Msa1450410:three_prime_UTR
TGGCTGAAACTGAAATATAC+TGG 0.393741 tig0032904:+14793 Msa1450410:three_prime_UTR
AGAGCTTCTTACGTGGGCTT+TGG 0.394876 tig0032904:+10869 Msa1450410:CDS
TTTGCCATACATGAAGTATT+CGG 0.401556 tig0032904:-13294 Msa1450410:intergenic
CGGCGGCTCCGGTGGCCGGA+CGG 0.402950 tig0032904:+10812 Msa1450410:CDS
ACTAAGCCTCCTTAATTTGT+TGG 0.405583 tig0032904:+14499 Msa1450410:three_prime_UTR
ATGTTGCTGATACAAGTTCT+TGG 0.407366 tig0032904:-12360 Msa1450410:intergenic
ATAAAGAATAAAGATGATAG+TGG 0.408236 tig0032904:-13667 Msa1450410:intergenic
GCAAGTCCACTTATAAATAG+TGG 0.408517 tig0032904:+13508 Msa1450410:three_prime_UTR
ACCTTTCCACTATTTATAAG+TGG 0.408565 tig0032904:-13514 Msa1450410:intergenic
TCGCGCGTGAACAGTTTATC+AGG 0.411494 tig0032904:-12078 Msa1450410:intergenic
TTTGATCGACAACGTGGAAA+TGG 0.415908 tig0032904:+11683 Msa1450410:CDS
TTTCCTATTTCCGAATCTAC+TGG 0.416167 tig0032904:-12243 Msa1450410:intergenic
ATGACGGCGGCTCCGGTGGC+CGG 0.417279 tig0032904:+10808 Msa1450410:CDS
AAGGTTTGTGTCATGAACTA+AGG 0.420705 tig0032904:-10664 Msa1450410:intergenic
TGCTTCTTGAGCTTCTTTCA+GGG 0.420899 tig0032904:+13354 Msa1450410:three_prime_UTR
TGCTCTTATTCCAGTAGATT+CGG 0.424228 tig0032904:+12233 Msa1450410:three_prime_UTR
ACAGTTTATCAGGTAATTGC+CGG 0.424665 tig0032904:-12068 Msa1450410:intergenic
ATGCTACAACAACAATAGTT+AGG 0.429695 tig0032904:-10618 Msa1450410:intergenic
TAGTATTTGTGATAAAGCTC+AGG 0.431150 tig0032904:+12179 Msa1450410:three_prime_UTR
CAATTGACACATGGTTTGCT+TGG 0.431286 tig0032904:+11722 Msa1450410:CDS
GGCATAAAATGGGTGAGAAA+TGG 0.434079 tig0032904:+14815 Msa1450410:three_prime_UTR
AAAACATTATTTCCCTCATC+GGG 0.435477 tig0032904:+12639 Msa1450410:three_prime_UTR
CATCAGAGTTGCTCAATTTG+TGG 0.436786 tig0032904:+11868 Msa1450410:three_prime_UTR
CTCCGGTGGCCGGACGGTGG+TGG 0.437422 tig0032904:+10818 Msa1450410:CDS
CAACGTGGAAATGGTTTGCT+TGG 0.440705 tig0032904:+11692 Msa1450410:CDS
ATCGTTGCTTTCTCTTGTTA+AGG 0.443839 tig0032904:+11051 Msa1450410:CDS
AAGATCGACGTCTATGCATT+TGG 0.445303 tig0032904:+13326 Msa1450410:three_prime_UTR
TACCTGAGGTTCAAAATCTT+CGG 0.445535 tig0032904:-12976 Msa1450410:intergenic
ACTTGTGATGACGGCGGCTC+CGG 0.448748 tig0032904:+10801 Msa1450410:CDS
CCCACTGGACCACTGAGATC+TGG 0.450377 tig0032904:-12101 Msa1450410:intergenic
TCATCCTTTACAAAACTCAA+TGG 0.452003 tig0032904:-14571 Msa1450410:intergenic
TCTGTTCTTAATGTTTATGA+AGG 0.452441 tig0032904:+11082 Msa1450410:CDS
TGTTATGTATGCATCCTTGT+TGG 0.457943 tig0032904:+14773 Msa1450410:three_prime_UTR
AGCAAGAACACAACAGTCCT+TGG 0.458238 tig0032904:-11494 Msa1450410:intergenic
AAATATACTGGGCATAAAAT+GGG 0.460940 tig0032904:+14805 Msa1450410:three_prime_UTR
TGGTGGCAATGAGAATTCTT+TGG 0.463358 tig0032904:+11961 Msa1450410:three_prime_UTR
TCATAGTTATCACCTAAGTT+AGG 0.468512 tig0032904:-13553 Msa1450410:intergenic
TAGAGGACTATTTGAGAGGC+AGG 0.468835 tig0032904:+14258 Msa1450410:three_prime_UTR
TTGTGTCATGAACTAAGGTT+TGG 0.469604 tig0032904:-10659 Msa1450410:intergenic
GTCAACAGAATCTTGTTATG+TGG 0.470257 tig0032904:+13408 Msa1450410:three_prime_UTR
ATAAGTGTTGATTATCCAAA+AGG 0.471314 tig0032904:+13386 Msa1450410:three_prime_UTR
TTATCAGGTAATTGCCGGTC+TGG 0.473424 tig0032904:-12063 Msa1450410:intergenic
CAGAGATATAAGGTTGCAAC+AGG 0.474302 tig0032904:+12867 Msa1450410:three_prime_UTR
CACCACCACCGTCCGGCCAC+CGG 0.476638 tig0032904:-10820 Msa1450410:intergenic
CAGATGTTGCTGGAACCTTT+GGG 0.477885 tig0032904:+13145 Msa1450410:three_prime_UTR
CAGGTTTCAAAGCTACTTCA+AGG 0.480604 tig0032904:+14062 Msa1450410:intron
ACTATTGTTGTTGTAGCATA+GGG 0.483607 tig0032904:+10622 Msa1450410:five_prime_UTR
GTTTCCTAATTATTAAGGTA+AGG 0.484044 tig0032904:-14478 Msa1450410:intergenic
CTCAGTCAAAACTTGATGCT+CGG 0.484748 tig0032904:-14286 Msa1450410:intergenic
CGAGAATGGCAATCTACTCT+TGG 0.486488 tig0032904:+12670 Msa1450410:three_prime_UTR
TCTTTCAAAACATCATATGA+AGG 0.488930 tig0032904:-12575 Msa1450410:intergenic
CGGGAAAGGAGGAAGTAGTA+AGG 0.489104 tig0032904:+12499 Msa1450410:three_prime_UTR
ATGATTTCAGAAAACTTGAT+CGG 0.490458 tig0032904:+12479 Msa1450410:intron
TCAGAAAACTTGATCGGGAA+AGG 0.491336 tig0032904:+12485 Msa1450410:intron
TTCAGATCATAGTTTAGCTA+AGG 0.491935 tig0032904:+11816 Msa1450410:exon
ATGATCATCCCGTGATTCAT+CGG 0.493047 tig0032904:+12925 Msa1450410:three_prime_UTR
TGATTTCAGAAAACTTGATC+GGG 0.493304 tig0032904:+12480 Msa1450410:intron
GAAGGATCTGAAGTCACATC+TGG 0.493969 tig0032904:+11935 Msa1450410:three_prime_UTR
GGTGGCCGGACGGTGGTGGT+GGG 0.495939 tig0032904:+10822 Msa1450410:CDS
TGATCATCCCGTGATTCATC+GGG 0.496058 tig0032904:+12926 Msa1450410:three_prime_UTR
CACGTAAGAAGCTCTTTGCT+TGG 0.496383 tig0032904:-10861 Msa1450410:intergenic
TCAACTGTTGCTGATGTACA+AGG 0.497164 tig0032904:+11562 Msa1450410:CDS
AACTATTGTTGTTGTAGCAT+AGG 0.499068 tig0032904:+10621 Msa1450410:five_prime_UTR
ATAACGTGCACAGATGTTGC+TGG 0.506097 tig0032904:+13135 Msa1450410:three_prime_UTR
AAAACCCTCGCGAGTTTGGT+TGG 0.506585 tig0032904:+12841 Msa1450410:three_prime_UTR
AGGATTCTGTAATTTGAAGC+AGG 0.507651 tig0032904:+11102 Msa1450410:CDS
CCCGCATCAGTTGTCTGTAC+AGG 0.507889 tig0032904:-11992 Msa1450410:intergenic
AAGACTAGAAGTCAATGCTT+TGG 0.508167 tig0032904:+14109 Msa1450410:three_prime_UTR
TCACTCCCACCACCACCGTC+CGG 0.511995 tig0032904:-10827 Msa1450410:intergenic
CGGCTCCGGTGGCCGGACGG+TGG 0.513016 tig0032904:+10815 Msa1450410:CDS
AAAGTATTCCAAAGAATTGG+AGG 0.514225 tig0032904:+12294 Msa1450410:three_prime_UTR
CCACTTACAATGCTCATTGG+TGG 0.518254 tig0032904:-13643 Msa1450410:intergenic
GCGGCAACCTCATTGCCCAC+TGG 0.525389 tig0032904:-12116 Msa1450410:intergenic
TGTGATGACGGCGGCTCCGG+TGG 0.526329 tig0032904:+10804 Msa1450410:CDS
GTCCATTGTACATGACATGA+AGG 0.526440 tig0032904:-13702 Msa1450410:intergenic
AACTATGATCTGAAATCCGT+CGG 0.526646 tig0032904:-11807 Msa1450410:intergenic
TCATGTCATGTACAATGGAC+AGG 0.527105 tig0032904:+13705 Msa1450410:three_prime_UTR
TTGGTTAAAGTTGCTCAACC+TGG 0.530182 tig0032904:+10888 Msa1450410:CDS
TGCCATCACGAAGGCAGCCT+CGG 0.530284 tig0032904:-12526 Msa1450410:intergenic
CAATTGTTAGATCCTAACTT+AGG 0.530726 tig0032904:+13541 Msa1450410:three_prime_UTR
GGGCCACTTACAATGCTCAT+TGG 0.538675 tig0032904:-13646 Msa1450410:intergenic
TAATCTTGCACTGCTTGATG+TGG 0.540495 tig0032904:+14190 Msa1450410:three_prime_UTR
ACTTTGCTCAACACTATACA+TGG 0.541807 tig0032904:-14228 Msa1450410:intergenic
TCAAACGTGCTCCAAAAGCT+AGG 0.550921 tig0032904:+13620 Msa1450410:three_prime_UTR
TATGTAATAATGCAATATGG+TGG 0.551576 tig0032904:+14991 Msa1450410:three_prime_UTR
TTTACATCCCGATGAATCAC+GGG 0.552297 tig0032904:-12933 Msa1450410:intergenic
GTCTATGATTTCTTATCAAG+AGG 0.554091 tig0032904:+12692 Msa1450410:three_prime_UTR
TACCGAGGCTGCCTTCGTGA+TGG 0.554450 tig0032904:+12524 Msa1450410:three_prime_UTR
GTTTGTGTAGAAATTGTGAA+TGG 0.554951 tig0032904:+11016 Msa1450410:intron
TAATTATTAAGGTAAGGAGG+AGG 0.555454 tig0032904:-14472 Msa1450410:intergenic
GTAGAAATTGTGAATGGAGA+TGG 0.558948 tig0032904:+11022 Msa1450410:intron
AGCTTGAATTGAAACCTGGT+TGG 0.560105 tig0032904:+12017 Msa1450410:three_prime_UTR
AGATGTGACTGTATATTACT+TGG 0.560396 tig0032904:-14167 Msa1450410:intergenic
ACCAGATCTCAGTGGTCCAG+TGG 0.560833 tig0032904:+12100 Msa1450410:three_prime_UTR
TGTGTACAAGCGTGACAGCT+TGG 0.562962 tig0032904:+11534 Msa1450410:CDS
ATGTAATAATGCAATATGGT+GGG 0.563568 tig0032904:+14992 Msa1450410:three_prime_UTR
TCAACAGAATCTTGTTATGT+GGG 0.565952 tig0032904:+13409 Msa1450410:three_prime_UTR
CCACCAATGAGCATTGTAAG+TGG 0.566515 tig0032904:+13643 Msa1450410:three_prime_UTR
CTTTACATCCCGATGAATCA+CGG 0.566789 tig0032904:-12934 Msa1450410:intergenic
TAGAAATTGTGAATGGAGAT+GGG 0.571352 tig0032904:+11023 Msa1450410:intron
CCTGTACAGACAACTGATGC+GGG 0.573206 tig0032904:+11992 Msa1450410:three_prime_UTR
TGACGAAGTAGCGGCCAACC+AGG 0.573621 tig0032904:-12031 Msa1450410:intergenic
TATTTCAGTTTCAGCCAACA+AGG 0.578428 tig0032904:-14787 Msa1450410:intergenic
TCCACTTATAAATAGTGGAA+AGG 0.579731 tig0032904:+13513 Msa1450410:three_prime_UTR
TCCTAATTATTAAGGTAAGG+AGG 0.581344 tig0032904:-14475 Msa1450410:intergenic
AAAGCTTATGGAAGATGCAA+AGG 0.581577 tig0032904:-14390 Msa1450410:intergenic
AGCCTTGAAGAGAACATTCA+CGG 0.582493 tig0032904:+12716 Msa1450410:three_prime_UTR
TCATGATGAGATGGAAAGAA+TGG 0.584179 tig0032904:+13576 Msa1450410:three_prime_UTR
ACTATGATCTGAAATCCGTC+GGG 0.585172 tig0032904:-11806 Msa1450410:intergenic
TAAAGAATAAAGATGATAGT+GGG 0.588251 tig0032904:-13666 Msa1450410:intergenic
TAACTATGATCATGATGAGA+TGG 0.593593 tig0032904:+13567 Msa1450410:three_prime_UTR
CAACAACAATAGTTAGGAGG+AGG 0.595212 tig0032904:-10612 Msa1450410:intergenic
CTTGTTTATCATGATCAACA+TGG 0.598491 tig0032904:-12155 Msa1450410:intergenic
AGTATTTGTGATAAAGCTCA+GGG 0.599563 tig0032904:+12180 Msa1450410:three_prime_UTR
AAGTATTCCAAAGAATTGGA+GGG 0.602831 tig0032904:+12295 Msa1450410:three_prime_UTR
GGCTGAAACTGAAATATACT+GGG 0.603609 tig0032904:+14794 Msa1450410:three_prime_UTR
CTACAACAACAATAGTTAGG+AGG 0.605858 tig0032904:-10615 Msa1450410:intergenic
GTGACTTCAGATCCTTCTGA+AGG 0.606310 tig0032904:-11929 Msa1450410:intergenic
CAAGCAAAGAGCTTCTTACG+TGG 0.606352 tig0032904:+10862 Msa1450410:CDS
TCTCGAAGCAAAACCCGATG+AGG 0.607635 tig0032904:-12652 Msa1450410:intergenic
GCCCTTCATGTCATGTACAA+TGG 0.608827 tig0032904:+13700 Msa1450410:three_prime_UTR
GGTTCAATTCAATTGACACA+TGG 0.610218 tig0032904:+11713 Msa1450410:CDS
AGGACTATTTGAGAGGCAGG+TGG 0.610626 tig0032904:+14261 Msa1450410:three_prime_UTR
GAAAACTTGATCGGGAAAGG+AGG 0.613251 tig0032904:+12488 Msa1450410:intron
TGTGTCCAACCAAACTCGCG+AGG 0.615194 tig0032904:-12846 Msa1450410:intergenic
TCATTAGAGGACTATTTGAG+AGG 0.616468 tig0032904:+14254 Msa1450410:three_prime_UTR
TCTGCAGAAGAGCCTTCAGA+AGG 0.617401 tig0032904:+11917 Msa1450410:three_prime_UTR
GCCTGTACAGACAACTGATG+CGG 0.617810 tig0032904:+11991 Msa1450410:three_prime_UTR
TGCCATACATGAAGTATTCG+GGG 0.617850 tig0032904:-13292 Msa1450410:intergenic
GGACAGCTTGAATTGAAACC+TGG 0.618955 tig0032904:+12013 Msa1450410:three_prime_UTR
CCAGATCTCAGTGGTCCAGT+GGG 0.622840 tig0032904:+12101 Msa1450410:three_prime_UTR
AATTATTAAGGTAAGGAGGA+GGG 0.624040 tig0032904:-14471 Msa1450410:intergenic
ATCTTCGGATAGTAATACGT+TGG 0.626548 tig0032904:-12961 Msa1450410:intergenic
ATTGTTGTTGTAGCATAGGG+TGG 0.626837 tig0032904:+10625 Msa1450410:five_prime_UTR
GCGATATTGATGCAATCAAG+TGG 0.626895 tig0032904:+14084 Msa1450410:three_prime_UTR
CTCGAAGCAAAACCCGATGA+GGG 0.629063 tig0032904:-12651 Msa1450410:intergenic
CAGTGGTCCAGTGGGCAATG+AGG 0.634781 tig0032904:+12109 Msa1450410:three_prime_UTR
ACTTAATGCTGATCCTGATG+AGG 0.635176 tig0032904:+11781 Msa1450410:CDS
AGAGCAACAACAAGATCACC+AGG 0.636218 tig0032904:-10906 Msa1450410:intergenic
AGCACGTTTGATACAAAGAG+TGG 0.639847 tig0032904:-13608 Msa1450410:intergenic
GTGTCCAACCAAACTCGCGA+GGG 0.641208 tig0032904:-12845 Msa1450410:intergenic
CGATATTGATGCAATCAAGT+GGG 0.641258 tig0032904:+14085 Msa1450410:three_prime_UTR
TCTTGCTGTGAATAATGGAA+AGG 0.644367 tig0032904:+11510 Msa1450410:CDS
TCCATTGTACATGACATGAA+GGG 0.650215 tig0032904:-13701 Msa1450410:intergenic
GCCATACATGAAGTATTCGG+GGG 0.651556 tig0032904:-13291 Msa1450410:intergenic
TGTGACTGTATATTACTTGG+TGG 0.652223 tig0032904:-14164 Msa1450410:intergenic
TAAAGCTCAGGGTCAATCTG+TGG 0.656231 tig0032904:+12191 Msa1450410:three_prime_UTR
AAGCAAAGAGCTTCTTACGT+GGG 0.657040 tig0032904:+10863 Msa1450410:CDS
GTGGCTTTAGATAGTGAAAG+TGG 0.657721 tig0032904:+12210 Msa1450410:three_prime_UTR
TCTGAAGTCACATCTGGTGA+TGG 0.661318 tig0032904:+11941 Msa1450410:three_prime_UTR
GATCCTGATGAGGAACCCGA+CGG 0.667135 tig0032904:+11791 Msa1450410:CDS
GAAGTCACATCTGGTGATGG+TGG 0.672465 tig0032904:+11944 Msa1450410:three_prime_UTR
GGAAGTAGTAAGGTTTACCG+AGG 0.672673 tig0032904:+12509 Msa1450410:three_prime_UTR
CAAGTTCTTTGCCATCACGA+AGG 0.672806 tig0032904:-12535 Msa1450410:intergenic
AAAGGGTTTGATCGACAACG+TGG 0.683684 tig0032904:+11677 Msa1450410:intron
ACTAGCCCAACAAATTAAGG+AGG 0.687236 tig0032904:-14505 Msa1450410:intergenic
CACGCGCGACCAGATCTCAG+TGG 0.743340 tig0032904:+12092 Msa1450410:three_prime_UTR
ATATAGCGTACACAACACAA+AGG 0.750051 tig0032904:-10683 Msa1450400:intergenic
TGGTATGAAAGATTTCTACG+CGG 0.833105 tig0032904:-12135 Msa1450400:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTTTATTTTGTTG+AGG + tig0032904:10965-10984 Msa1450410:intron 10.0%
!! TAATCTTGTTAAAAATAAAA+TGG - tig0032904:12451-12470 Msa1450410:intergenic 10.0%
!!! TTTTTGATTTTCAATTTTGT+AGG + tig0032904:11309-11328 Msa1450410:intron 15.0%
!! ACACTTAAAACAAAATTATA+TGG - tig0032904:13883-13902 Msa1450410:intergenic 15.0%
!!! TTTGAAGTATAATGAAAAAA+GGG - tig0032904:14534-14553 Msa1450410:intergenic 15.0%
!!! ATTTGAAGTATAATGAAAAA+AGG - tig0032904:14535-14554 Msa1450410:intergenic 15.0%
!! AAAATTGTTGTCATATTATT+AGG - tig0032904:14637-14656 Msa1450410:intergenic 15.0%
!! AGAAAATAAAAGTTATACTA+TGG + tig0032904:14904-14923 Msa1450410:three_prime_UTR 15.0%
!! AATTATGTAATAATGCAATA+TGG + tig0032904:14988-15007 Msa1450410:three_prime_UTR 15.0%
!! TTGTTTATTAGGATCATAAT+TGG - tig0032904:11245-11264 Msa1450410:intergenic 20.0%
!!! TTTTGGATTTTTCTTAGTTT+TGG + tig0032904:11585-11604 Msa1450410:intron 20.0%
!!! TTTGTTTTTTTGCTTTTGAA+AGG + tig0032904:11659-11678 Msa1450410:intron 20.0%
!!! TTGTTTTTTTGCTTTTGAAA+GGG + tig0032904:11660-11679 Msa1450410:intron 20.0%
!!! ATACTTTTTAAATTGCATTC+AGG - tig0032904:12281-12300 Msa1450410:intergenic 20.0%
!!! TAAAAAGTATTCCAAAGAAT+TGG + tig0032904:12291-12310 Msa1450410:three_prime_UTR 20.0%
!! TTTCTATATCATGCTAAATT+AGG + tig0032904:12810-12829 Msa1450410:intron 20.0%
!! TTCTATATCATGCTAAATTA+GGG + tig0032904:12811-12830 Msa1450410:intron 20.0%
!! TAAAGAATAAAGATGATAGT+GGG - tig0032904:13669-13688 Msa1450410:intergenic 20.0%
!! ATAAAGAATAAAGATGATAG+TGG - tig0032904:13670-13689 Msa1450410:intergenic 20.0%
!!! TTATTTTCTTGTTCTAGTAA+TGG + tig0032904:13798-13817 Msa1450410:intron 20.0%
!!! TCTTCATGGTTTTTATTAAA+TGG + tig0032904:14417-14436 Msa1450410:three_prime_UTR 20.0%
!!! CTTCATGGTTTTTATTAAAT+GGG + tig0032904:14418-14437 Msa1450410:three_prime_UTR 20.0%
!!! TTCATGGTTTTTATTAAATG+GGG + tig0032904:14419-14438 Msa1450410:three_prime_UTR 20.0%
!! TTGAAGTATAATGAAAAAAG+GGG - tig0032904:14533-14552 Msa1450410:intergenic 20.0%
!!! AAGTTGTATTTTAGAGTATT+AGG + tig0032904:14596-14615 Msa1450410:three_prime_UTR 20.0%
!! TTGATTGACAATGAATTTAA+CGG + tig0032904:14704-14723 Msa1450410:three_prime_UTR 20.0%
!! CTAAAAATACATACCTATAA+GGG + tig0032904:14845-14864 Msa1450410:three_prime_UTR 20.0%
! TGATGATTGATATGTGAAAT+GGG + tig0032904:10994-11013 Msa1450410:intron 25.0%
! TCTGTTCTTAATGTTTATGA+AGG + tig0032904:11082-11101 Msa1450410:CDS 25.0%
! TGTCTCAAATTGATTACAAA+TGG + tig0032904:11189-11208 Msa1450410:intron 25.0%
! TTTATTAGGATCATAATTGG+AGG - tig0032904:11242-11261 Msa1450410:intergenic 25.0%
!! TTGATTTTCAATTTTGTAGG+TGG + tig0032904:11312-11331 Msa1450410:intron 25.0%
!! TTCATGGTTTTCATAGAATT+CGG + tig0032904:11431-11450 Msa1450410:CDS 25.0%
!!! TTTCTTAGTTTTGGTTAATG+TGG + tig0032904:11594-11613 Msa1450410:intron 25.0%
!! TACTTTTGTATTCTTACTGA+GGG - tig0032904:11762-11781 Msa1450410:intergenic 25.0%
! TTAATACTAAGAAACAAACC+AGG - tig0032904:12397-12416 Msa1450410:intergenic 25.0%
! TAATTAAGTTTGACTGTGTT+CGG + tig0032904:12414-12433 Msa1450410:intron 25.0%
! ATGATTTCAGAAAACTTGAT+CGG + tig0032904:12479-12498 Msa1450410:intron 25.0%
! TCTTTCAAAACATCATATGA+AGG - tig0032904:12578-12597 Msa1450410:intergenic 25.0%
!!! TGTTTTGAAAGAGTTTCTTT+TGG + tig0032904:12586-12605 Msa1450410:three_prime_UTR 25.0%
! AAAAACATTATTTCCCTCAT+CGG + tig0032904:12638-12657 Msa1450410:three_prime_UTR 25.0%
! ATCACATAGAAAAGTTAAAG+GGG - tig0032904:12765-12784 Msa1450410:intergenic 25.0%
! GATCACATAGAAAAGTTAAA+GGG - tig0032904:12766-12785 Msa1450410:intergenic 25.0%
! TGATCACATAGAAAAGTTAA+AGG - tig0032904:12767-12786 Msa1450410:intergenic 25.0%
! TTATCTATCTTTCGATTGAT+TGG + tig0032904:13000-13019 Msa1450410:intron 25.0%
! TTTGGATAATCAACACTTAT+GGG - tig0032904:13386-13405 Msa1450410:intergenic 25.0%
!! TTTTGGATAATCAACACTTA+TGG - tig0032904:13387-13406 Msa1450410:intergenic 25.0%
!!! ATAAGTGTTGATTATCCAAA+AGG + tig0032904:13386-13405 Msa1450410:three_prime_UTR 25.0%
! TGCTATAATGCATAATGATT+AGG - tig0032904:13462-13481 Msa1450410:intergenic 25.0%
!! TAATCGAAAAGATATTTTGC+AGG + tig0032904:13486-13505 Msa1450410:intron 25.0%
! TAGGAAAAGCTAAACAAAAA+AGG - tig0032904:13738-13757 Msa1450410:intergenic 25.0%
!!! TTAGCTTTTCCTAAATAATG+TGG + tig0032904:13745-13764 Msa1450410:three_prime_UTR 25.0%
! ATTCAAGTACCACATTATTT+AGG - tig0032904:13757-13776 Msa1450410:intergenic 25.0%
!!! TGGACCTTCTAATTTTTTAA+GGG - tig0032904:13863-13882 Msa1450410:intergenic 25.0%
!!! ATGGACCTTCTAATTTTTTA+AGG - tig0032904:13864-13883 Msa1450410:intergenic 25.0%
! CAACTGTTTGATTATTTCAA+TGG + tig0032904:13903-13922 Msa1450410:intron 25.0%
!! TCATGGTTTTTATTAAATGG+GGG + tig0032904:14420-14439 Msa1450410:three_prime_UTR 25.0%
! GTTTCCTAATTATTAAGGTA+AGG - tig0032904:14481-14500 Msa1450410:intergenic 25.0%
! GCTTAGTTTCCTAATTATTA+AGG - tig0032904:14486-14505 Msa1450410:intergenic 25.0%
! TGAAGTATAATGAAAAAAGG+GGG - tig0032904:14532-14551 Msa1450410:intergenic 25.0%
! CAATGAATTTAACGGAATTA+AGG + tig0032904:14712-14731 Msa1450410:three_prime_UTR 25.0%
! AAATATACTGGGCATAAAAT+GGG + tig0032904:14805-14824 Msa1450410:three_prime_UTR 25.0%
!!! AGGTATGTATTTTTAGCAAT+TGG - tig0032904:14841-14860 Msa1450410:intergenic 25.0%
! GCTAAAAATACATACCTATA+AGG + tig0032904:14844-14863 Msa1450410:three_prime_UTR 25.0%
! TGCTTTTAACCGAAAAGTTA+TGG + tig0032904:10564-10583 Msa1450410:five_prime_UTR 30.0%
ATGCTACAACAACAATAGTT+AGG - tig0032904:10621-10640 Msa1450410:intergenic 30.0%
! AACTATTGTTGTTGTAGCAT+AGG + tig0032904:10621-10640 Msa1450410:five_prime_UTR 30.0%
! ACTATTGTTGTTGTAGCATA+GGG + tig0032904:10622-10641 Msa1450410:five_prime_UTR 30.0%
ATGCAAAAAACTTGTGATGA+CGG + tig0032904:10792-10811 Msa1450410:CDS 30.0%
GTGATGATTGATATGTGAAA+TGG + tig0032904:10993-11012 Msa1450410:intron 30.0%
GTTTGTGTAGAAATTGTGAA+TGG + tig0032904:11016-11035 Msa1450410:intron 30.0%
TAGAAATTGTGAATGGAGAT+GGG + tig0032904:11023-11042 Msa1450410:intron 30.0%
! ATCATCGGTGAAGAAAATTT+TGG + tig0032904:11360-11379 Msa1450410:CDS 30.0%
TTGTGCTAAAAAGTTATCCA+AGG + tig0032904:11477-11496 Msa1450410:CDS 30.0%
! GTACTTTTGTATTCTTACTG+AGG - tig0032904:11763-11782 Msa1450410:intergenic 30.0%
TTCAGATCATAGTTTAGCTA+AGG + tig0032904:11816-11835 Msa1450410:exon 30.0%
! TAGTTTAGCTAAGGTTTTAC+TGG + tig0032904:11825-11844 Msa1450410:exon 30.0%
TAAAAATTCTGACGAAGTAG+CGG - tig0032904:12043-12062 Msa1450410:intergenic 30.0%
CTTGTTTATCATGATCAACA+TGG - tig0032904:12158-12177 Msa1450410:intergenic 30.0%
TAGTATTTGTGATAAAGCTC+AGG + tig0032904:12179-12198 Msa1450410:three_prime_UTR 30.0%
AGTATTTGTGATAAAGCTCA+GGG + tig0032904:12180-12199 Msa1450410:three_prime_UTR 30.0%
! AAAGTATTCCAAAGAATTGG+AGG + tig0032904:12294-12313 Msa1450410:three_prime_UTR 30.0%
AAGTATTCCAAAGAATTGGA+GGG + tig0032904:12295-12314 Msa1450410:three_prime_UTR 30.0%
TGATTTCAGAAAACTTGATC+GGG + tig0032904:12480-12499 Msa1450410:intron 30.0%
AAACATCATATGAAGGCTTT+AGG - tig0032904:12571-12590 Msa1450410:intergenic 30.0%
AAAACATTATTTCCCTCATC+GGG + tig0032904:12639-12658 Msa1450410:three_prime_UTR 30.0%
GTCTATGATTTCTTATCAAG+AGG + tig0032904:12692-12711 Msa1450410:three_prime_UTR 30.0%
TCACATAGAAAAGTTAAAGG+GGG - tig0032904:12764-12783 Msa1450410:intergenic 30.0%
GAAAGATAGATAAATACCTG+AGG - tig0032904:12993-13012 Msa1450410:intergenic 30.0%
! TTTATTCAGCTCTCTGATTT+TGG + tig0032904:13078-13097 Msa1450410:intron 30.0%
! AACGGATGATAAGTTTTTGA+TGG - tig0032904:13193-13212 Msa1450410:intergenic 30.0%
!!! GATCGCTCTAAAAACATTTT+CGG - tig0032904:13233-13252 Msa1450410:intergenic 30.0%
! TTTGCCATACATGAAGTATT+CGG - tig0032904:13297-13316 Msa1450410:intergenic 30.0%
! TCAACAGAATCTTGTTATGT+GGG + tig0032904:13409-13428 Msa1450410:three_prime_UTR 30.0%
! TCCACTTATAAATAGTGGAA+AGG + tig0032904:13513-13532 Msa1450410:three_prime_UTR 30.0%
ACCTTTCCACTATTTATAAG+TGG - tig0032904:13517-13536 Msa1450410:intergenic 30.0%
CAATTGTTAGATCCTAACTT+AGG + tig0032904:13541-13560 Msa1450410:three_prime_UTR 30.0%
TCATAGTTATCACCTAAGTT+AGG - tig0032904:13556-13575 Msa1450410:intergenic 30.0%
TAACTATGATCATGATGAGA+TGG + tig0032904:13567-13586 Msa1450410:three_prime_UTR 30.0%
TCAGCCCTTAAAAAATTAGA+AGG + tig0032904:13856-13875 Msa1450410:intron 30.0%
! GGGAATAAGAATACTTTTGA+CGG - tig0032904:13994-14013 Msa1450410:intergenic 30.0%
AGAATACATGAGAATTGCAA+CGG - tig0032904:14033-14052 Msa1450410:intergenic 30.0%
GCAATTCTCATGTATTCTAT+TGG + tig0032904:14035-14054 Msa1450410:intron 30.0%
AGATGTGACTGTATATTACT+TGG - tig0032904:14170-14189 Msa1450410:intergenic 30.0%
! CATGGTTTTTATTAAATGGG+GGG + tig0032904:14421-14440 Msa1450410:three_prime_UTR 30.0%
! TTTTATTAAATGGGGGGTTT+AGG + tig0032904:14427-14446 Msa1450410:three_prime_UTR 30.0%
AATTATTAAGGTAAGGAGGA+GGG - tig0032904:14474-14493 Msa1450410:intergenic 30.0%
TAATTATTAAGGTAAGGAGG+AGG - tig0032904:14475-14494 Msa1450410:intergenic 30.0%
TCCTCCTTACCTTAATAATT+AGG + tig0032904:14474-14493 Msa1450410:three_prime_UTR 30.0%
TCCTAATTATTAAGGTAAGG+AGG - tig0032904:14478-14497 Msa1450410:intergenic 30.0%
!! TAGTCCATTGAGTTTTGTAA+AGG + tig0032904:14567-14586 Msa1450410:three_prime_UTR 30.0%
TCATCCTTTACAAAACTCAA+TGG - tig0032904:14574-14593 Msa1450410:intergenic 30.0%
GAAATATACTGGGCATAAAA+TGG + tig0032904:14804-14823 Msa1450410:three_prime_UTR 30.0%
! ACGCTTCAACCATAACTTTT+CGG - tig0032904:10576-10595 Msa1450410:intergenic 35.0%
CTACAACAACAATAGTTAGG+AGG - tig0032904:10618-10637 Msa1450410:intergenic 35.0%
TTGTGTCATGAACTAAGGTT+TGG - tig0032904:10662-10681 Msa1450410:intergenic 35.0%
AAGGTTTGTGTCATGAACTA+AGG - tig0032904:10667-10686 Msa1450410:intergenic 35.0%
ATATAGCGTACACAACACAA+AGG - tig0032904:10686-10705 Msa1450410:intergenic 35.0%
!!! TTTTTTGTGTGTGTGAGTGA+AGG - tig0032904:10715-10734 Msa1450410:intergenic 35.0%
GTAGAAATTGTGAATGGAGA+TGG + tig0032904:11022-11041 Msa1450410:intron 35.0%
! ATCGTTGCTTTCTCTTGTTA+AGG + tig0032904:11051-11070 Msa1450410:CDS 35.0%
! AGGATTCTGTAATTTGAAGC+AGG + tig0032904:11102-11121 Msa1450410:CDS 35.0%
CTTAAGCTAAAAATTTGCCG+TGG + tig0032904:11337-11356 Msa1450410:CDS 35.0%
AAAAATTTGCCGTGGATCAT+CGG + tig0032904:11345-11364 Msa1450410:CDS 35.0%
TCTGCAACTCATGTTATTGT+TGG + tig0032904:11403-11422 Msa1450410:CDS 35.0%
!! GTTATTGTTGGAAGTGTTCA+TGG + tig0032904:11415-11434 Msa1450410:CDS 35.0%
! TGTGTTCTTGCTGTGAATAA+TGG + tig0032904:11505-11524 Msa1450410:CDS 35.0%
! TCTTGCTGTGAATAATGGAA+AGG + tig0032904:11510-11529 Msa1450410:CDS 35.0%
GGTTCAATTCAATTGACACA+TGG + tig0032904:11713-11732 Msa1450410:CDS 35.0%
! AACTATGATCTGAAATCCGT+CGG - tig0032904:11810-11829 Msa1450410:intergenic 35.0%
ACAGTTTATCAGGTAATTGC+CGG - tig0032904:12071-12090 Msa1450410:intergenic 35.0%
TGGTATGAAAGATTTCTACG+CGG - tig0032904:12138-12157 Msa1450410:intergenic 35.0%
TGCTCTTATTCCAGTAGATT+CGG + tig0032904:12233-12252 Msa1450410:three_prime_UTR 35.0%
ATTCCAGTAGATTCGGAAAT+AGG + tig0032904:12240-12259 Msa1450410:three_prime_UTR 35.0%
TTTCCTATTTCCGAATCTAC+TGG - tig0032904:12246-12265 Msa1450410:intergenic 35.0%
ATGTTGCTGATACAAGTTCT+TGG - tig0032904:12363-12382 Msa1450410:intergenic 35.0%
!!! GCAACATCAAATTTTTTGCC+TGG + tig0032904:12376-12395 Msa1450410:three_prime_UTR 35.0%
ATCTTCGGATAGTAATACGT+TGG - tig0032904:12964-12983 Msa1450410:intergenic 35.0%
TACCTGAGGTTCAAAATCTT+CGG - tig0032904:12979-12998 Msa1450410:intergenic 35.0%
AATCAAAGCAGACCAACATA+AGG - tig0032904:13060-13079 Msa1450410:intergenic 35.0%
!! ACTTGAAAGTACTAACCCAA+AGG - tig0032904:13163-13182 Msa1450410:intergenic 35.0%
GTAGCATGTATGGATAAGAA+CGG - tig0032904:13211-13230 Msa1450410:intergenic 35.0%
! AACATTTTCGGTAGCATGTA+TGG - tig0032904:13221-13240 Msa1450410:intergenic 35.0%
! TTGCCATACATGAAGTATTC+GGG - tig0032904:13296-13315 Msa1450410:intergenic 35.0%
! ACAAGATTCTGTTGACCTTT+TGG - tig0032904:13404-13423 Msa1450410:intergenic 35.0%
! GTCAACAGAATCTTGTTATG+TGG + tig0032904:13408-13427 Msa1450410:three_prime_UTR 35.0%
GCAAGTCCACTTATAAATAG+TGG + tig0032904:13508-13527 Msa1450410:three_prime_UTR 35.0%
TCATGATGAGATGGAAAGAA+TGG + tig0032904:13576-13595 Msa1450410:three_prime_UTR 35.0%
TCCATTGTACATGACATGAA+GGG - tig0032904:13704-13723 Msa1450410:intergenic 35.0%
CATGTATTCTATTGGACAAC+AGG + tig0032904:14043-14062 Msa1450410:intron 35.0%
CGATATTGATGCAATCAAGT+GGG + tig0032904:14085-14104 Msa1450410:three_prime_UTR 35.0%
AAGACTAGAAGTCAATGCTT+TGG + tig0032904:14109-14128 Msa1450410:three_prime_UTR 35.0%
TGTGACTGTATATTACTTGG+TGG - tig0032904:14167-14186 Msa1450410:intergenic 35.0%
ACTTTGCTCAACACTATACA+TGG - tig0032904:14231-14250 Msa1450410:intergenic 35.0%
! TGAGCAAAGTGTTTCATTAG+AGG + tig0032904:14241-14260 Msa1450410:three_prime_UTR 35.0%
TCATTAGAGGACTATTTGAG+AGG + tig0032904:14254-14273 Msa1450410:three_prime_UTR 35.0%
AAAGCTTATGGAAGATGCAA+AGG - tig0032904:14393-14412 Msa1450410:intergenic 35.0%
TTTGCATCTTCCATAAGCTT+TGG + tig0032904:14392-14411 Msa1450410:three_prime_UTR 35.0%
!! ACTAAGCCTCCTTAATTTGT+TGG + tig0032904:14499-14518 Msa1450410:three_prime_UTR 35.0%
!! CTAAGCCTCCTTAATTTGTT+GGG + tig0032904:14500-14519 Msa1450410:three_prime_UTR 35.0%
GGAATTAAGGTATGTGTATC+AGG + tig0032904:14725-14744 Msa1450410:three_prime_UTR 35.0%
TGTTATGTATGCATCCTTGT+TGG + tig0032904:14773-14792 Msa1450410:three_prime_UTR 35.0%
TATTTCAGTTTCAGCCAACA+AGG - tig0032904:14790-14809 Msa1450410:intergenic 35.0%
TGGCTGAAACTGAAATATAC+TGG + tig0032904:14793-14812 Msa1450410:three_prime_UTR 35.0%
GGCTGAAACTGAAATATACT+GGG + tig0032904:14794-14813 Msa1450410:three_prime_UTR 35.0%
CAACAACAATAGTTAGGAGG+AGG - tig0032904:10615-10634 Msa1450410:intergenic 40.0%
! ATTGTTGTTGTAGCATAGGG+TGG + tig0032904:10625-10644 Msa1450410:five_prime_UTR 40.0%
!! CAAAAAACTTGTGATGACGG+CGG + tig0032904:10795-10814 Msa1450410:CDS 40.0%
AAGCAAAGAGCTTCTTACGT+GGG + tig0032904:10863-10882 Msa1450410:CDS 40.0%
TTGGTTAAAGTTGCTCAACC+TGG + tig0032904:10888-10907 Msa1450410:CDS 40.0%
TTGTTGCTCTGCATGTTCTT+GGG + tig0032904:10919-10938 Msa1450410:CDS 40.0%
! TTTTCTTCACCGATGATCCA+CGG - tig0032904:11357-11376 Msa1450410:intergenic 40.0%
TCAACTGTTGCTGATGTACA+AGG + tig0032904:11562-11581 Msa1450410:CDS 40.0%
! GTTGCTGATGTACAAGGTTT+TGG + tig0032904:11568-11587 Msa1450410:intron 40.0%
TTTGATCGACAACGTGGAAA+TGG + tig0032904:11683-11702 Msa1450410:CDS 40.0%
!! CAATTGACACATGGTTTGCT+TGG + tig0032904:11722-11741 Msa1450410:CDS 40.0%
ACTTAATGCTGATCCTGATG+AGG + tig0032904:11781-11800 Msa1450410:CDS 40.0%
! ACTATGATCTGAAATCCGTC+GGG - tig0032904:11809-11828 Msa1450410:intergenic 40.0%
CATCAGAGTTGCTCAATTTG+TGG + tig0032904:11868-11887 Msa1450410:three_prime_UTR 40.0%
AAGGCTCTTCTGCAGATTTA+TGG - tig0032904:11913-11932 Msa1450410:intergenic 40.0%
TGGTGGCAATGAGAATTCTT+TGG + tig0032904:11961-11980 Msa1450410:three_prime_UTR 40.0%
AGCTTGAATTGAAACCTGGT+TGG + tig0032904:12017-12036 Msa1450410:three_prime_UTR 40.0%
! CGTCAGAATTTTTACCAGAC+CGG + tig0032904:12049-12068 Msa1450410:three_prime_UTR 40.0%
!! GTGGCTTTAGATAGTGAAAG+TGG + tig0032904:12210-12229 Msa1450410:three_prime_UTR 40.0%
ATGAAGTCCCTCCAATTCTT+TGG - tig0032904:12305-12324 Msa1450410:intergenic 40.0%
TCAGAAAACTTGATCGGGAA+AGG + tig0032904:12485-12504 Msa1450410:intron 40.0%
AGCCTTGAAGAGAACATTCA+CGG + tig0032904:12716-12735 Msa1450410:three_prime_UTR 40.0%
TACCGTGAATGTTCTCTTCA+AGG - tig0032904:12721-12740 Msa1450410:intergenic 40.0%
TGGTTGGACACAGAGATATA+AGG + tig0032904:12857-12876 Msa1450410:three_prime_UTR 40.0%
CAGAGATATAAGGTTGCAAC+AGG + tig0032904:12867-12886 Msa1450410:three_prime_UTR 40.0%
ATGATCATCCCGTGATTCAT+CGG + tig0032904:12925-12944 Msa1450410:three_prime_UTR 40.0%
TTTACATCCCGATGAATCAC+GGG - tig0032904:12936-12955 Msa1450410:intergenic 40.0%
CTTTACATCCCGATGAATCA+CGG - tig0032904:12937-12956 Msa1450410:intergenic 40.0%
! ATCCGAAGATTTTGAACCTC+AGG + tig0032904:12974-12993 Msa1450410:three_prime_UTR 40.0%
GTACCAATCAGTCCTTATGT+TGG + tig0032904:13045-13064 Msa1450410:intron 40.0%
AGACCAACATAAGGACTGAT+TGG - tig0032904:13051-13070 Msa1450410:intergenic 40.0%
! TCAGCTCTCTGATTTTGGAT+TGG + tig0032904:13083-13102 Msa1450410:intron 40.0%
!!! TGATTTTGGATTGGCTACGT+GGG + tig0032904:13092-13111 Msa1450410:three_prime_UTR 40.0%
ACAGGCGCAATACATAATTC+AGG - tig0032904:13255-13274 Msa1450410:intergenic 40.0%
CGCCTGTTTATGCAGTTATA+TGG + tig0032904:13268-13287 Msa1450410:intron 40.0%
GGCCATATAACTGCATAAAC+AGG - tig0032904:13273-13292 Msa1450410:intergenic 40.0%
! TGCCATACATGAAGTATTCG+GGG - tig0032904:13295-13314 Msa1450410:intergenic 40.0%
AAGATCGACGTCTATGCATT+TGG + tig0032904:13326-13345 Msa1450410:three_prime_UTR 40.0%
TGCTTCTTGAGCTTCTTTCA+GGG + tig0032904:13354-13373 Msa1450410:three_prime_UTR 40.0%
AGCACGTTTGATACAAAGAG+TGG - tig0032904:13611-13630 Msa1450410:intergenic 40.0%
GTCCATTGTACATGACATGA+AGG - tig0032904:13705-13724 Msa1450410:intergenic 40.0%
TCATGTCATGTACAATGGAC+AGG + tig0032904:13705-13724 Msa1450410:three_prime_UTR 40.0%
TCACACAAGCAAGCAAATCA+CGG - tig0032904:13929-13948 Msa1450410:intergenic 40.0%
ACGGACAAAGGACTAATGAA+GGG - tig0032904:14014-14033 Msa1450410:intergenic 40.0%
AACGGACAAAGGACTAATGA+AGG - tig0032904:14015-14034 Msa1450410:intergenic 40.0%
ATGAGAATTGCAACGGACAA+AGG - tig0032904:14026-14045 Msa1450410:intergenic 40.0%
CAGGTTTCAAAGCTACTTCA+AGG + tig0032904:14062-14081 Msa1450410:intron 40.0%
GCGATATTGATGCAATCAAG+TGG + tig0032904:14084-14103 Msa1450410:three_prime_UTR 40.0%
TAATCTTGCACTGCTTGATG+TGG + tig0032904:14190-14209 Msa1450410:three_prime_UTR 40.0%
CTCAGTCAAAACTTGATGCT+CGG - tig0032904:14289-14308 Msa1450410:intergenic 40.0%
CATGAAGATGCCAAAGCTTA+TGG - tig0032904:14405-14424 Msa1450410:intergenic 40.0%
! CATAAGCTTTGGCATCTTCA+TGG + tig0032904:14403-14422 Msa1450410:three_prime_UTR 40.0%
ACTAGCCCAACAAATTAAGG+AGG - tig0032904:14508-14527 Msa1450410:intergenic 40.0%
GGTACTAGCCCAACAAATTA+AGG - tig0032904:14511-14530 Msa1450410:intergenic 40.0%
GGCATAAAATGGGTGAGAAA+TGG + tig0032904:14815-14834 Msa1450410:three_prime_UTR 40.0%
CAACACAAAGCTTCCCTTAT+AGG - tig0032904:14861-14880 Msa1450410:intergenic 40.0%
CACGTAAGAAGCTCTTTGCT+TGG - tig0032904:10864-10883 Msa1450410:intergenic 45.0%
CAAGCAAAGAGCTTCTTACG+TGG + tig0032904:10862-10881 Msa1450410:CDS 45.0%
AGAGCAACAACAAGATCACC+AGG - tig0032904:10909-10928 Msa1450410:intergenic 45.0%
GTTGTTGCTCTGCATGTTCT+TGG + tig0032904:10918-10937 Msa1450410:CDS 45.0%
CTGCATGTTCTTGGGACTAA+TGG + tig0032904:10927-10946 Msa1450410:CDS 45.0%
AGCAAGAACACAACAGTCCT+TGG - tig0032904:11497-11516 Msa1450410:intergenic 45.0%
AAAGGGTTTGATCGACAACG+TGG + tig0032904:11677-11696 Msa1450410:intron 45.0%
!! CAACGTGGAAATGGTTTGCT+TGG + tig0032904:11692-11711 Msa1450410:CDS 45.0%
GTGACTTCAGATCCTTCTGA+AGG - tig0032904:11932-11951 Msa1450410:intergenic 45.0%
! GAAGGATCTGAAGTCACATC+TGG + tig0032904:11935-11954 Msa1450410:three_prime_UTR 45.0%
! TCTGAAGTCACATCTGGTGA+TGG + tig0032904:11941-11960 Msa1450410:three_prime_UTR 45.0%
GGACAGCTTGAATTGAAACC+TGG + tig0032904:12013-12032 Msa1450410:three_prime_UTR 45.0%
TTATCAGGTAATTGCCGGTC+TGG - tig0032904:12066-12085 Msa1450410:intergenic 45.0%
TAAAGCTCAGGGTCAATCTG+TGG + tig0032904:12191-12210 Msa1450410:three_prime_UTR 45.0%
GAAAACTTGATCGGGAAAGG+AGG + tig0032904:12488-12507 Msa1450410:intron 45.0%
GGAAGTAGTAAGGTTTACCG+AGG + tig0032904:12509-12528 Msa1450410:three_prime_UTR 45.0%
CAAGTTCTTTGCCATCACGA+AGG - tig0032904:12538-12557 Msa1450410:intergenic 45.0%
! ATCGGGTTTTGCTTCGAGAA+TGG + tig0032904:12656-12675 Msa1450410:three_prime_UTR 45.0%
CGAGAATGGCAATCTACTCT+TGG + tig0032904:12670-12689 Msa1450410:three_prime_UTR 45.0%
!! AAGTTAAAGGGGGTGTTGCA+AGG - tig0032904:12754-12773 Msa1450410:intergenic 45.0%
AAGAAAAACCCTCGCGAGTT+TGG + tig0032904:12837-12856 Msa1450410:three_prime_UTR 45.0%
TGATCATCCCGTGATTCATC+GGG + tig0032904:12926-12945 Msa1450410:three_prime_UTR 45.0%
!!! CTGATTTTGGATTGGCTACG+TGG + tig0032904:13091-13110 Msa1450410:three_prime_UTR 45.0%
ATAACGTGCACAGATGTTGC+TGG + tig0032904:13135-13154 Msa1450410:three_prime_UTR 45.0%
ACAGATGTTGCTGGAACCTT+TGG + tig0032904:13144-13163 Msa1450410:three_prime_UTR 45.0%
CAGATGTTGCTGGAACCTTT+GGG + tig0032904:13145-13164 Msa1450410:three_prime_UTR 45.0%
! GCCATACATGAAGTATTCGG+GGG - tig0032904:13294-13313 Msa1450410:intergenic 45.0%
GTGCTTCTTGAGCTTCTTTC+AGG + tig0032904:13353-13372 Msa1450410:three_prime_UTR 45.0%
TCAAACGTGCTCCAAAAGCT+AGG + tig0032904:13620-13639 Msa1450410:three_prime_UTR 45.0%
CCACTTACAATGCTCATTGG+TGG - tig0032904:13646-13665 Msa1450410:intergenic 45.0%
! CCACCAATGAGCATTGTAAG+TGG + tig0032904:13643-13662 Msa1450410:three_prime_UTR 45.0%
GCCCTTCATGTCATGTACAA+TGG + tig0032904:13700-13719 Msa1450410:three_prime_UTR 45.0%
ATTTACGATTCCCTGACTGC+AGG + tig0032904:13949-13968 Msa1450410:intron 45.0%
TTTACGATTCCCTGACTGCA+GGG + tig0032904:13950-13969 Msa1450410:intron 45.0%
! ATACTTTTGACGGTGCTCGA+AGG - tig0032904:13984-14003 Msa1450410:intergenic 45.0%
TAGAGGACTATTTGAGAGGC+AGG + tig0032904:14258-14277 Msa1450410:three_prime_UTR 45.0%
!!! TTTTATTTTTTTTGTTTATT+AGG - tig0032904:11256-11275 Msa1450410:intergenic 5.0%
AGAGCTTCTTACGTGGGCTT+TGG + tig0032904:10869-10888 Msa1450410:CDS 50.0%
TGTGTACAAGCGTGACAGCT+TGG + tig0032904:11534-11553 Msa1450410:CDS 50.0%
TCTGCAGAAGAGCCTTCAGA+AGG + tig0032904:11917-11936 Msa1450410:three_prime_UTR 50.0%
!! GAAGTCACATCTGGTGATGG+TGG + tig0032904:11944-11963 Msa1450410:three_prime_UTR 50.0%
GCCTGTACAGACAACTGATG+CGG + tig0032904:11991-12010 Msa1450410:three_prime_UTR 50.0%
CCTGTACAGACAACTGATGC+GGG + tig0032904:11992-12011 Msa1450410:three_prime_UTR 50.0%
TCGCGCGTGAACAGTTTATC+AGG - tig0032904:12081-12100 Msa1450410:intergenic 50.0%
CGGGAAAGGAGGAAGTAGTA+AGG + tig0032904:12499-12518 Msa1450410:three_prime_UTR 50.0%
CTCGAAGCAAAACCCGATGA+GGG - tig0032904:12654-12673 Msa1450410:intergenic 50.0%
TCTCGAAGCAAAACCCGATG+AGG - tig0032904:12655-12674 Msa1450410:intergenic 50.0%
AAAACCCTCGCGAGTTTGGT+TGG + tig0032904:12841-12860 Msa1450410:three_prime_UTR 50.0%
GCCCCCGAATACTTCATGTA+TGG + tig0032904:13290-13309 Msa1450410:three_prime_UTR 50.0%
!! CTCATTGGTGGCCTAGCTTT+TGG - tig0032904:13634-13653 Msa1450410:intergenic 50.0%
GGGCCACTTACAATGCTCAT+TGG - tig0032904:13649-13668 Msa1450410:intergenic 50.0%
ATTCCCTGACTGCAGGGAAT+TGG + tig0032904:13956-13975 Msa1450410:intron 50.0%
TTCCCTGACTGCAGGGAATT+GGG + tig0032904:13957-13976 Msa1450410:intron 50.0%
AGGACTATTTGAGAGGCAGG+TGG + tig0032904:14261-14280 Msa1450410:three_prime_UTR 50.0%
! AATGGGGGGTTTAGGATGAG+AGG + tig0032904:14435-14454 Msa1450410:three_prime_UTR 50.0%
!! GGTGGTGGTGGGAGTGAAAA+TGG + tig0032904:10833-10852 Msa1450410:CDS 55.0%
GATCCTGATGAGGAACCCGA+CGG + tig0032904:11791-11810 Msa1450410:CDS 55.0%
AATCCGTCGGGTTCCTCATC+AGG - tig0032904:11797-11816 Msa1450410:intergenic 55.0%
CCCGCATCAGTTGTCTGTAC+AGG - tig0032904:11995-12014 Msa1450410:intergenic 55.0%
ACCAGATCTCAGTGGTCCAG+TGG + tig0032904:12100-12119 Msa1450410:three_prime_UTR 55.0%
CCAGATCTCAGTGGTCCAGT+GGG + tig0032904:12101-12120 Msa1450410:three_prime_UTR 55.0%
GTGTCCAACCAAACTCGCGA+GGG - tig0032904:12848-12867 Msa1450410:intergenic 55.0%
TGTGTCCAACCAAACTCGCG+AGG - tig0032904:12849-12868 Msa1450410:intergenic 55.0%
GACCCAATTCCCTGCAGTCA+GGG - tig0032904:13962-13981 Msa1450410:intergenic 55.0%
!! ACTTGTGATGACGGCGGCTC+CGG + tig0032904:10801-10820 Msa1450410:CDS 60.0%
TGACGAAGTAGCGGCCAACC+AGG - tig0032904:12034-12053 Msa1450410:intergenic 60.0%
CCCACTGGACCACTGAGATC+TGG - tig0032904:12104-12123 Msa1450410:intergenic 60.0%
CAGTGGTCCAGTGGGCAATG+AGG + tig0032904:12109-12128 Msa1450410:three_prime_UTR 60.0%
TACCGAGGCTGCCTTCGTGA+TGG + tig0032904:12524-12543 Msa1450410:three_prime_UTR 60.0%
TGCCATCACGAAGGCAGCCT+CGG - tig0032904:12529-12548 Msa1450410:intergenic 60.0%
GGACCCAATTCCCTGCAGTC+AGG - tig0032904:13963-13982 Msa1450410:intergenic 60.0%
TCACTCCCACCACCACCGTC+CGG - tig0032904:10830-10849 Msa1450410:intergenic 65.0%
CACGCGCGACCAGATCTCAG+TGG + tig0032904:12092-12111 Msa1450410:three_prime_UTR 65.0%
GCGGCAACCTCATTGCCCAC+TGG - tig0032904:12119-12138 Msa1450410:intergenic 65.0%
!! TGTGATGACGGCGGCTCCGG+TGG + tig0032904:10804-10823 Msa1450410:CDS 70.0%
ATGACGGCGGCTCCGGTGGC+CGG + tig0032904:10808-10827 Msa1450410:CDS 75.0%
CACCACCACCGTCCGGCCAC+CGG - tig0032904:10823-10842 Msa1450410:intergenic 75.0%
! GGTGGCCGGACGGTGGTGGT+GGG + tig0032904:10822-10841 Msa1450410:CDS 75.0%
! CTCCGGTGGCCGGACGGTGG+TGG + tig0032904:10818-10837 Msa1450410:CDS 80.0%
!! CGGTGGCCGGACGGTGGTGG+TGG + tig0032904:10821-10840 Msa1450410:CDS 80.0%
! CGGCGGCTCCGGTGGCCGGA+CGG + tig0032904:10812-10831 Msa1450410:CDS 85.0%
! CGGCTCCGGTGGCCGGACGG+TGG + tig0032904:10815-10834 Msa1450410:CDS 85.0%
Chromosome Type Strat End Strand Name
tig0032904 gene 10542 15013 10542 ID=Msa1450410;Name=Msa1450410
tig0032904 mRNA 10542 15013 10542 ID=Msa1450410-mRNA-1;Parent=Msa1450410;Name=Msa1450410-mRNA-1;_AED=0.16;_eAED=0.16;_QI=250|1|1|1|0.77|0.8|10|2414|219
tig0032904 exon 10542 10948 10542 ID=Msa1450410-mRNA-1:exon:17462;Parent=Msa1450410-mRNA-1
tig0032904 exon 11026 11123 11026 ID=Msa1450410-mRNA-1:exon:17463;Parent=Msa1450410-mRNA-1
tig0032904 exon 11331 11583 11331 ID=Msa1450410-mRNA-1:exon:17464;Parent=Msa1450410-mRNA-1
tig0032904 exon 11681 12397 11681 ID=Msa1450410-mRNA-1:exon:17465;Parent=Msa1450410-mRNA-1
tig0032904 exon 12489 12737 12489 ID=Msa1450410-mRNA-1:exon:17466;Parent=Msa1450410-mRNA-1
tig0032904 exon 12832 12995 12832 ID=Msa1450410-mRNA-1:exon:17467;Parent=Msa1450410-mRNA-1
tig0032904 exon 13087 13166 13087 ID=Msa1450410-mRNA-1:exon:17468;Parent=Msa1450410-mRNA-1
tig0032904 exon 13283 13430 13283 ID=Msa1450410-mRNA-1:exon:17469;Parent=Msa1450410-mRNA-1
tig0032904 exon 13508 13766 13508 ID=Msa1450410-mRNA-1:exon:17470;Parent=Msa1450410-mRNA-1
tig0032904 exon 14065 15013 14065 ID=Msa1450410-mRNA-1:exon:17471;Parent=Msa1450410-mRNA-1
tig0032904 five_prime_UTR 10542 10791 10542 ID=Msa1450410-mRNA-1:five_prime_utr;Parent=Msa1450410-mRNA-1
tig0032904 CDS 10792 10948 10792 ID=Msa1450410-mRNA-1:cds;Parent=Msa1450410-mRNA-1
tig0032904 CDS 11026 11123 11026 ID=Msa1450410-mRNA-1:cds;Parent=Msa1450410-mRNA-1
tig0032904 CDS 11331 11583 11331 ID=Msa1450410-mRNA-1:cds;Parent=Msa1450410-mRNA-1
tig0032904 CDS 11681 11832 11681 ID=Msa1450410-mRNA-1:cds;Parent=Msa1450410-mRNA-1
tig0032904 three_prime_UTR 11833 12397 11833 ID=Msa1450410-mRNA-1:three_prime_utr;Parent=Msa1450410-mRNA-1
tig0032904 three_prime_UTR 12489 12737 12489 ID=Msa1450410-mRNA-1:three_prime_utr;Parent=Msa1450410-mRNA-1
tig0032904 three_prime_UTR 12832 12995 12832 ID=Msa1450410-mRNA-1:three_prime_utr;Parent=Msa1450410-mRNA-1
tig0032904 three_prime_UTR 13087 13166 13087 ID=Msa1450410-mRNA-1:three_prime_utr;Parent=Msa1450410-mRNA-1
tig0032904 three_prime_UTR 13283 13430 13283 ID=Msa1450410-mRNA-1:three_prime_utr;Parent=Msa1450410-mRNA-1
tig0032904 three_prime_UTR 13508 13766 13508 ID=Msa1450410-mRNA-1:three_prime_utr;Parent=Msa1450410-mRNA-1
tig0032904 three_prime_UTR 14065 15013 14065 ID=Msa1450410-mRNA-1:three_prime_utr;Parent=Msa1450410-mRNA-1
Gene Sequence

>Msa1450410

ATGCAAAAAACTTGTGATGACGGCGGCTCCGGTGGCCGGACGGTGGTGGTGGGAGTGAAAATGGATTCACCAAGCAAAGAGCTTCTTACGTGGGCTTTGGTTAAAGTTGCTCAACCTGGTGATCTTGTTGTTGCTCTGCATGTTCTTGGGACTAATGAAATTGTGAATGGAGATGGGAAATCATCGTTGCTTTCTCTTGTTAAGGCTTTTGACTCTGTTCTTAATGTTTATGAAGGATTCTGTAATTTGAAGCAGGTGGATCTTAAGCTAAAAATTTGCCGTGGATCATCGGTGAAGAAAATTTTGGTACGAGAAGCAAATGCTTATTCTGCAACTCATGTTATTGTTGGAAGTGTTCATGGTTTTCATAGAATTCGGTCATCTAGCTCTGTAGCTAAGTATTGTGCTAAAAAGTTATCCAAGGACTGTTGTGTTCTTGCTGTGAATAATGGAAAGGTTGTGTACAAGCGTGACAGCTTGGCAGCATCAACTGTTGCTGATGTACAAGGGTTTGATCGACAACGTGGAAATGGTTTGCTTGGTTCAATTCAATTGACACATGGTTTGCTTGGTTCGATTCAATTGACCCTCAGTAAGAATACAAAAGTACTTAATGCTGATCCTGATGAGGAACCCGACGGATTTCAGATCATAGTTTAG
Protein sequence

>Msa1450410

MQKTCDDGGSGGRTVVVGVKMDSPSKELLTWALVKVAQPGDLVVALHVLGTNEIVNGDGKSSLLSLVKAFDSVLNVYEGFCNLKQVDLKLKICRGSSVKKILVREANAYSATHVIVGSVHGFHRIRSSSSVAKYCAKKLSKDCCVLAVNNGKVVYKRDSLAASTVADVQGFDRQRGNGLLGSIQLTHGLLGSIQLTLSKNTKVLNADPDEEPDGFQIIV*