AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1451330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1451330 MtrunA17_Chr4g0057161 98.000 300 4 1 1 300 1 298 0.0 603
Msa1451330 MtrunA17_Chr8g0359401 82.807 285 49 0 13 297 383 667 2.81e-177 505
Msa1451330 MtrunA17_Chr2g0314601 81.724 290 53 0 8 297 379 668 5.49e-176 502
Msa1451330 MtrunA17_Chr1g0147841 78.873 284 60 0 14 297 371 654 2.82e-167 479
Msa1451330 MtrunA17_Chr3g0097081 61.616 99 38 0 7 105 160 258 9.20e-33 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1451330 AT1G63000.1 83.566 286 47 0 14 299 13 298 0.0 512
Msa1451330 AT1G78570.1 80.808 297 52 2 6 297 372 668 9.79e-177 504
Msa1451330 AT1G53500.1 78.621 290 62 0 8 297 377 666 1.47e-157 455
Msa1451330 AT3G14790.1 78.157 293 64 0 5 297 371 663 2.26e-157 454
Msa1451330 AT3G14790.2 78.157 293 64 0 5 297 374 666 2.76e-157 454

Find 103 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATGATCTTCATCTTCT+TGG 0.210029 tig0033396:+16509 Msa1451330:CDS
TCTAAAATGGTCATCGAGTT+TGG 0.262726 tig0033396:-17404 None:intergenic
TCAAGCTCATTGTTACTTCT+TGG 0.275443 tig0033396:-17605 None:intergenic
GCTGGGAAACCCATGATATT+TGG 0.303776 tig0033396:-16143 None:intergenic
CAAGCTCATTGTTACTTCTT+GGG 0.312171 tig0033396:-17604 None:intergenic
CAAGCTTTAACCTTCTGATT+TGG 0.317551 tig0033396:-17701 None:intergenic
GGGAAGAAGTTCATCTAAAA+TGG 0.318463 tig0033396:-17417 None:intergenic
CATCTTCTTGGATCCGGGAT+TGG 0.324588 tig0033396:+16521 Msa1451330:CDS
CCCCTCAAGTTTCTCATATA+CGG 0.329932 tig0033396:+16188 Msa1451330:CDS
TTTAATGCTGCTGGTGTTAC+GGG 0.336811 tig0033396:+16347 Msa1451330:CDS
CTCAAGAGTGAAGTTCTTCC+AGG 0.342280 tig0033396:-17558 None:intergenic
GAGAATCAAATCAATCGATA+TGG 0.362687 tig0033396:-15988 None:intergenic
AGAGAAACCTCACTGGAATC+TGG 0.362925 tig0033396:+17459 Msa1451330:CDS
TTGGATAAATCAGATGAAAT+AGG 0.363426 tig0033396:-17329 None:intergenic
TCCTTGATAGACAATAGTTC+TGG 0.368427 tig0033396:-17653 None:intergenic
GAAGATACTCCTAATTTCAT+TGG 0.368560 tig0033396:+16554 Msa1451330:CDS
TCTTGAGAATCGATCTTCAT+TGG 0.371797 tig0033396:+16289 Msa1451330:CDS
TATCTACAACCTTCTCATAT+CGG 0.390642 tig0033396:-17379 None:intergenic
GTTTAATGCTGCTGGTGTTA+CGG 0.392378 tig0033396:+16346 Msa1451330:CDS
CTTTCTCACTCCAAATATCA+TGG 0.393877 tig0033396:+16133 Msa1451330:exon
GATCTTCATCTTCTTGGATC+CGG 0.397535 tig0033396:+16515 Msa1451330:CDS
TTCTTTATAACGTAAATAAA+CGG 0.400489 tig0033396:-16010 None:intergenic
CGTATTCGAATTGGATCCCT+TGG 0.400642 tig0033396:-16256 None:intergenic
GACCCGACCCGTATTCGAAT+TGG 0.406229 tig0033396:-16265 None:intergenic
TTTATTTACGTTATAAAGAA+AGG 0.407222 tig0033396:+16013 Msa1451330:five_prime_UTR
TCGGAGGAGGAGGATGGTGC+TGG 0.407640 tig0033396:-16161 None:intergenic
ATCTTCATCTTCTTGGATCC+GGG 0.408563 tig0033396:+16516 Msa1451330:CDS
ATCTGGAACTTCACAAATCC+TGG 0.419713 tig0033396:+17476 Msa1451330:CDS
CTTTGCCATCTCAATTGAGA+TGG 0.421086 tig0033396:-17438 None:intergenic
TCCAGAACTATTGTCTATCA+AGG 0.428839 tig0033396:+17652 Msa1451330:CDS
GGCTGGATAGGAGGCCTCTT+AGG 0.432855 tig0033396:+16218 Msa1451330:CDS
CAAGGGATCCAATTCGAATA+CGG 0.434923 tig0033396:+16257 Msa1451330:CDS
GAGGGGAGTGTCGGAGGAGG+AGG 0.437559 tig0033396:-16171 None:intergenic
GAAAATAATACCCCATATAC+AGG 0.441120 tig0033396:-18009 None:intergenic
GTCTCACACCAGTCCACGTT+GGG 0.462588 tig0033396:-16374 None:intergenic
ATCTACAACCTTCTCATATC+GGG 0.463578 tig0033396:-17378 None:intergenic
TTCTTCCTTAAAACCAATCC+CGG 0.465949 tig0033396:-16534 None:intergenic
TCATATACGGCCGCACCGGC+TGG 0.469750 tig0033396:+16201 Msa1451330:CDS
TCATTGTGGCTAACAACTCC+AGG 0.479957 tig0033396:-17494 None:intergenic
ACATACCATAGCCTTGGTCT+TGG 0.487323 tig0033396:-16588 None:intergenic
AGACACGGAAATAGTATTGC+TGG 0.489889 tig0033396:-16095 None:intergenic
AAGCTCATTGTTACTTCTTG+GGG 0.490161 tig0033396:-17603 None:intergenic
CGGTACAGAGTTTGCCTAAG+AGG 0.492780 tig0033396:-16232 None:intergenic
CGGGTCGACCCAACGTGGAC+TGG 0.494677 tig0033396:+16366 Msa1451330:CDS
TAACAATAAGTCCACGTTCA+CGG 0.500654 tig0033396:-16457 None:intergenic
CTATCCGTGCTAATGTGGTT+GGG 0.501072 tig0033396:+16411 Msa1451330:CDS
ATCCAATTCGAATACGGGTC+GGG 0.505835 tig0033396:+16263 Msa1451330:CDS
AAGGTTAAAGCTTGAGTACA+AGG 0.509109 tig0033396:+17710 Msa1451330:exon
GGCAAACTCTGTACCGCCCA+AGG 0.511999 tig0033396:+16239 Msa1451330:CDS
TTACTCCAAGACCAAGGCTA+TGG 0.515471 tig0033396:+16583 Msa1451330:CDS
GCCGTATATGAGAAACTTGA+GGG 0.516441 tig0033396:-16189 None:intergenic
CTTGAGGGGAGTGTCGGAGG+AGG 0.523463 tig0033396:-16174 None:intergenic
CACTCTTGAGGAACAAGCTA+AGG 0.523587 tig0033396:+17571 Msa1451330:CDS
CGGAGGAGGAGGATGGTGCT+GGG 0.526896 tig0033396:-16160 None:intergenic
TTTCTCACTCCAAATATCAT+GGG 0.527605 tig0033396:+16134 Msa1451330:exon
AGTAACAATGAGCTTGATGC+GGG 0.528741 tig0033396:+17611 Msa1451330:CDS
GTATTCGAATTGGATCCCTT+GGG 0.529711 tig0033396:-16255 None:intergenic
TTGATTTATTTCAGTTTGTG+TGG 0.531764 tig0033396:+17765 Msa1451330:three_prime_UTR
TTTGCCATCTCAATTGAGAT+GGG 0.537632 tig0033396:-17437 None:intergenic
AAGGGATCCAATTCGAATAC+GGG 0.542692 tig0033396:+16258 Msa1451330:CDS
TGTGATGAAATTGCGAGGGT+TGG 0.546615 tig0033396:-17348 None:intergenic
ATTTGGAGTGAGAAAGTGAG+AGG 0.546724 tig0033396:-16126 None:intergenic
AATGTAACATACCATAGCCT+TGG 0.554984 tig0033396:-16594 None:intergenic
TTTCTCATATACGGCCGCAC+CGG 0.558062 tig0033396:+16197 Msa1451330:CDS
TCTTCCCATCTCAATTGAGA+TGG 0.561970 tig0033396:+17433 Msa1451330:CDS
ATATCGATTCAAGCTTCACC+TGG 0.568911 tig0033396:+17540 Msa1451330:CDS
TAAGAGGCCTCCTATCCAGC+CGG 0.572784 tig0033396:-16216 None:intergenic
GGAGTGTCGGAGGAGGAGGA+TGG 0.576494 tig0033396:-16167 None:intergenic
AAACTTGAGGGGAGTGTCGG+AGG 0.580469 tig0033396:-16177 None:intergenic
ACTATCCGTGCTAATGTGGT+TGG 0.582347 tig0033396:+16410 Msa1451330:CDS
GCAGCATTAAACACGTGTGT+TGG 0.584742 tig0033396:-16335 None:intergenic
TGATTCAGCAGGATCAATGC+AGG 0.585648 tig0033396:+17972 Msa1451330:three_prime_UTR
TACGGCCGCACCGGCTGGAT+AGG 0.586868 tig0033396:+16206 Msa1451330:CDS
GAGAAACTTGAGGGGAGTGT+CGG 0.586872 tig0033396:-16180 None:intergenic
CTGGAAGAACTTCACTCTTG+AGG 0.589081 tig0033396:+17559 Msa1451330:CDS
GGCCGTATATGAGAAACTTG+AGG 0.591913 tig0033396:-16190 None:intergenic
AATAGGCATCCTCACTCTCA+AGG 0.595727 tig0033396:-17312 None:intergenic
TGATGCGGGAAAATTGAAGA+AGG 0.598610 tig0033396:+17625 Msa1451330:CDS
TGTCTTCAAGCCAAATCAGA+AGG 0.603264 tig0033396:+17691 Msa1451330:CDS
ATGGCAAAGAGAAACCTCAC+TGG 0.606695 tig0033396:+17452 Msa1451330:CDS
GGAGTGAACAATGATTCAGC+AGG 0.617978 tig0033396:+17961 Msa1451330:three_prime_UTR
AAAGATCACCCGATATGAGA+AGG 0.619965 tig0033396:+17370 Msa1451330:CDS
TTCGAATTGGATCCCTTGGG+CGG 0.621824 tig0033396:-16252 None:intergenic
GCAAACTCTGTACCGCCCAA+GGG 0.622614 tig0033396:+16240 Msa1451330:CDS
ACACACGTGTTTAATGCTGC+TGG 0.626893 tig0033396:+16338 Msa1451330:CDS
ATCTTTGTGATGAAATTGCG+AGG 0.632297 tig0033396:-17353 None:intergenic
GGCCTCCTATCCAGCCGGTG+CGG 0.634921 tig0033396:-16211 None:intergenic
GATCCAATTCGAATACGGGT+CGG 0.635111 tig0033396:+16262 Msa1451330:CDS
AAGTAACAATGAGCTTGATG+CGG 0.635352 tig0033396:+17610 Msa1451330:CDS
TGAATCATATGAACGGAGAG+AGG 0.636169 tig0033396:-17804 None:intergenic
ATGTATGCACCTTGAGAGTG+AGG 0.639312 tig0033396:+17303 Msa1451330:CDS
TGAAACTATCCGTGCTAATG+TGG 0.665772 tig0033396:+16406 Msa1451330:CDS
CAGGCACTGAATCATATGAA+CGG 0.668390 tig0033396:-17811 None:intergenic
GAGTCCCAACCACATTAGCA+CGG 0.686328 tig0033396:-16415 None:intergenic
ACATTTGTAAGATTTCATTG+TGG 0.688248 tig0033396:-17508 None:intergenic
AGTCTCACACCAGTCCACGT+TGG 0.693140 tig0033396:-16375 None:intergenic
AATACGCATCGAGAGAGCAG+AGG 0.696381 tig0033396:+16061 Msa1451330:five_prime_UTR
CACAGGTAGAGGATCTGCTG+AGG 0.704068 tig0033396:+17270 Msa1451330:intron
TGTTACGGGTCGACCCAACG+TGG 0.704086 tig0033396:+16361 Msa1451330:CDS
TCAATCGATATGGAGCACGA+TGG 0.707527 tig0033396:-15978 None:intergenic
TGAAGTTCCAGATTCCAGTG+AGG 0.720062 tig0033396:-17466 None:intergenic
TCTTTGTGATGAAATTGCGA+GGG 0.720478 tig0033396:-17352 None:intergenic
CCGTATATGAGAAACTTGAG+GGG 0.728448 tig0033396:-16188 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTTATAACGTAAATAAA+CGG - tig0033396:16013-16032 None:intergenic 15.0%
!! TTTATTTACGTTATAAAGAA+AGG + tig0033396:16013-16032 Msa1451330:five_prime_UTR 15.0%
!! TATAATATAAAACAGATCTA+AGG - tig0033396:16788-16807 None:intergenic 15.0%
!! CAACATATAAATAAAAATAC+AGG - tig0033396:17833-17852 None:intergenic 15.0%
!! TTCTTTATAACGTAAATAAA+CGG - tig0033396:16013-16032 None:intergenic 15.0%
!! TTTATTTACGTTATAAAGAA+AGG + tig0033396:16013-16032 Msa1451330:five_prime_UTR 15.0%
!! TATAATATAAAACAGATCTA+AGG - tig0033396:16788-16807 None:intergenic 15.0%
!! CAACATATAAATAAAAATAC+AGG - tig0033396:17833-17852 None:intergenic 15.0%
!!! ATTGTTATTAATTTTGCTAC+TGG + tig0033396:16473-16492 Msa1451330:CDS 20.0%
!! TAAAAAGAACCAATGAAATT+AGG - tig0033396:16566-16585 None:intergenic 20.0%
!!! TGTTTAAGATTGATGTTTTT+TGG + tig0033396:16642-16661 Msa1451330:intron 20.0%
!! ATATCAAACAAATCAAAACA+CGG - tig0033396:16888-16907 None:intergenic 20.0%
!!! ATTGTTATTAATTTTGCTAC+TGG + tig0033396:16473-16492 Msa1451330:CDS 20.0%
!! TAAAAAGAACCAATGAAATT+AGG - tig0033396:16566-16585 None:intergenic 20.0%
!!! TGTTTAAGATTGATGTTTTT+TGG + tig0033396:16642-16661 Msa1451330:intron 20.0%
!! ATATCAAACAAATCAAAACA+CGG - tig0033396:16888-16907 None:intergenic 20.0%
!! TATGGTATGTTACATTGATT+TGG + tig0033396:16601-16620 Msa1451330:intron 25.0%
!!! ATGCTGATTTGTTGAATTAT+TGG + tig0033396:16716-16735 Msa1451330:intron 25.0%
!!! GATTTGTTGAATTATTGGTT+AGG + tig0033396:16721-16740 Msa1451330:intron 25.0%
!!! TTACTTTTATATGCAGTAGT+TGG + tig0033396:16915-16934 Msa1451330:intron 25.0%
!! CTTAAGAGACTAATGTTTTT+CGG - tig0033396:17117-17136 None:intergenic 25.0%
!! AAAAACATTAGTCTCTTAAG+CGG + tig0033396:17117-17136 Msa1451330:intron 25.0%
! ACATTAACAAAAAGAAAGCA+AGG + tig0033396:17191-17210 Msa1451330:intron 25.0%
! TTGGATAAATCAGATGAAAT+AGG - tig0033396:17332-17351 None:intergenic 25.0%
! ACATTTGTAAGATTTCATTG+TGG - tig0033396:17511-17530 None:intergenic 25.0%
! TTGATTTATTTCAGTTTGTG+TGG + tig0033396:17765-17784 Msa1451330:three_prime_UTR 25.0%
!! TATGGTATGTTACATTGATT+TGG + tig0033396:16601-16620 Msa1451330:intron 25.0%
!!! ATGCTGATTTGTTGAATTAT+TGG + tig0033396:16716-16735 Msa1451330:intron 25.0%
!!! GATTTGTTGAATTATTGGTT+AGG + tig0033396:16721-16740 Msa1451330:intron 25.0%
!!! TTACTTTTATATGCAGTAGT+TGG + tig0033396:16915-16934 Msa1451330:intron 25.0%
!! CTTAAGAGACTAATGTTTTT+CGG - tig0033396:17117-17136 None:intergenic 25.0%
!! AAAAACATTAGTCTCTTAAG+CGG + tig0033396:17117-17136 Msa1451330:intron 25.0%
! ACATTAACAAAAAGAAAGCA+AGG + tig0033396:17191-17210 Msa1451330:intron 25.0%
! TTGGATAAATCAGATGAAAT+AGG - tig0033396:17332-17351 None:intergenic 25.0%
! ACATTTGTAAGATTTCATTG+TGG - tig0033396:17511-17530 None:intergenic 25.0%
! TTGATTTATTTCAGTTTGTG+TGG + tig0033396:17765-17784 Msa1451330:three_prime_UTR 25.0%
GAGAATCAAATCAATCGATA+TGG - tig0033396:15991-16010 None:intergenic 30.0%
TTTCTCACTCCAAATATCAT+GGG + tig0033396:16134-16153 Msa1451330:exon 30.0%
TATGATGATCTTCATCTTCT+TGG + tig0033396:16509-16528 Msa1451330:CDS 30.0%
GAAGATACTCCTAATTTCAT+TGG + tig0033396:16554-16573 Msa1451330:CDS 30.0%
TAGTCATTCGTTTGTAACAT+TGG - tig0033396:17065-17084 None:intergenic 30.0%
TTAACAAAAAGAAAGCAAGG+TGG + tig0033396:17194-17213 Msa1451330:intron 30.0%
GTTCACAAAAAAGGAAAACA+AGG - tig0033396:17251-17270 None:intergenic 30.0%
!! TTTTCCTTTTTTGTGAACAC+AGG + tig0033396:17253-17272 Msa1451330:intron 30.0%
TATCTACAACCTTCTCATAT+CGG - tig0033396:17382-17401 None:intergenic 30.0%
GAGAATCAAATCAATCGATA+TGG - tig0033396:15991-16010 None:intergenic 30.0%
TTTCTCACTCCAAATATCAT+GGG + tig0033396:16134-16153 Msa1451330:exon 30.0%
TATGATGATCTTCATCTTCT+TGG + tig0033396:16509-16528 Msa1451330:CDS 30.0%
GAAGATACTCCTAATTTCAT+TGG + tig0033396:16554-16573 Msa1451330:CDS 30.0%
TAGTCATTCGTTTGTAACAT+TGG - tig0033396:17065-17084 None:intergenic 30.0%
TTAACAAAAAGAAAGCAAGG+TGG + tig0033396:17194-17213 Msa1451330:intron 30.0%
GTTCACAAAAAAGGAAAACA+AGG - tig0033396:17251-17270 None:intergenic 30.0%
!! TTTTCCTTTTTTGTGAACAC+AGG + tig0033396:17253-17272 Msa1451330:intron 30.0%
TATCTACAACCTTCTCATAT+CGG - tig0033396:17382-17401 None:intergenic 30.0%
CTTTCTCACTCCAAATATCA+TGG + tig0033396:16133-16152 Msa1451330:exon 35.0%
TCTTGAGAATCGATCTTCAT+TGG + tig0033396:16289-16308 Msa1451330:CDS 35.0%
TAACAATAAGTCCACGTTCA+CGG - tig0033396:16460-16479 None:intergenic 35.0%
TTCTTCCTTAAAACCAATCC+CGG - tig0033396:16537-16556 None:intergenic 35.0%
! TTCTTTTTACTCCAAGACCA+AGG + tig0033396:16577-16596 Msa1451330:CDS 35.0%
AATGTAACATACCATAGCCT+TGG - tig0033396:16597-16616 None:intergenic 35.0%
TGTTACAAACGAATGACTAC+AGG + tig0033396:17066-17085 Msa1451330:intron 35.0%
GTTACAAACGAATGACTACA+GGG + tig0033396:17067-17086 Msa1451330:intron 35.0%
! AGAAAGCAAGGTGGTATTTT+AGG + tig0033396:17203-17222 Msa1451330:intron 35.0%
TCTACCTGTGTTCACAAAAA+AGG - tig0033396:17260-17279 None:intergenic 35.0%
!!! TTTTTTGTGAACACAGGTAG+AGG + tig0033396:17259-17278 Msa1451330:intron 35.0%
TCTTTGTGATGAAATTGCGA+GGG - tig0033396:17355-17374 None:intergenic 35.0%
ATCTTTGTGATGAAATTGCG+AGG - tig0033396:17356-17375 None:intergenic 35.0%
ATCTACAACCTTCTCATATC+GGG - tig0033396:17381-17400 None:intergenic 35.0%
! TCTAAAATGGTCATCGAGTT+TGG - tig0033396:17407-17426 None:intergenic 35.0%
GGGAAGAAGTTCATCTAAAA+TGG - tig0033396:17420-17439 None:intergenic 35.0%
TTTGCCATCTCAATTGAGAT+GGG - tig0033396:17440-17459 None:intergenic 35.0%
! AAGCTCATTGTTACTTCTTG+GGG - tig0033396:17606-17625 None:intergenic 35.0%
! CAAGCTCATTGTTACTTCTT+GGG - tig0033396:17607-17626 None:intergenic 35.0%
! TCAAGCTCATTGTTACTTCT+TGG - tig0033396:17608-17627 None:intergenic 35.0%
AAGTAACAATGAGCTTGATG+CGG + tig0033396:17610-17629 Msa1451330:CDS 35.0%
TCCAGAACTATTGTCTATCA+AGG + tig0033396:17652-17671 Msa1451330:CDS 35.0%
TCCTTGATAGACAATAGTTC+TGG - tig0033396:17656-17675 None:intergenic 35.0%
! CAAGCTTTAACCTTCTGATT+TGG - tig0033396:17704-17723 None:intergenic 35.0%
AAGGTTAAAGCTTGAGTACA+AGG + tig0033396:17710-17729 Msa1451330:exon 35.0%
CTTTCTCACTCCAAATATCA+TGG + tig0033396:16133-16152 Msa1451330:exon 35.0%
TCTTGAGAATCGATCTTCAT+TGG + tig0033396:16289-16308 Msa1451330:CDS 35.0%
TAACAATAAGTCCACGTTCA+CGG - tig0033396:16460-16479 None:intergenic 35.0%
TTCTTCCTTAAAACCAATCC+CGG - tig0033396:16537-16556 None:intergenic 35.0%
! TTCTTTTTACTCCAAGACCA+AGG + tig0033396:16577-16596 Msa1451330:CDS 35.0%
AATGTAACATACCATAGCCT+TGG - tig0033396:16597-16616 None:intergenic 35.0%
TGTTACAAACGAATGACTAC+AGG + tig0033396:17066-17085 Msa1451330:intron 35.0%
GTTACAAACGAATGACTACA+GGG + tig0033396:17067-17086 Msa1451330:intron 35.0%
! AGAAAGCAAGGTGGTATTTT+AGG + tig0033396:17203-17222 Msa1451330:intron 35.0%
TCTACCTGTGTTCACAAAAA+AGG - tig0033396:17260-17279 None:intergenic 35.0%
!!! TTTTTTGTGAACACAGGTAG+AGG + tig0033396:17259-17278 Msa1451330:intron 35.0%
TCTTTGTGATGAAATTGCGA+GGG - tig0033396:17355-17374 None:intergenic 35.0%
ATCTTTGTGATGAAATTGCG+AGG - tig0033396:17356-17375 None:intergenic 35.0%
ATCTACAACCTTCTCATATC+GGG - tig0033396:17381-17400 None:intergenic 35.0%
! TCTAAAATGGTCATCGAGTT+TGG - tig0033396:17407-17426 None:intergenic 35.0%
GGGAAGAAGTTCATCTAAAA+TGG - tig0033396:17420-17439 None:intergenic 35.0%
TTTGCCATCTCAATTGAGAT+GGG - tig0033396:17440-17459 None:intergenic 35.0%
! AAGCTCATTGTTACTTCTTG+GGG - tig0033396:17606-17625 None:intergenic 35.0%
! CAAGCTCATTGTTACTTCTT+GGG - tig0033396:17607-17626 None:intergenic 35.0%
! TCAAGCTCATTGTTACTTCT+TGG - tig0033396:17608-17627 None:intergenic 35.0%
AAGTAACAATGAGCTTGATG+CGG + tig0033396:17610-17629 Msa1451330:CDS 35.0%
TCCAGAACTATTGTCTATCA+AGG + tig0033396:17652-17671 Msa1451330:CDS 35.0%
TCCTTGATAGACAATAGTTC+TGG - tig0033396:17656-17675 None:intergenic 35.0%
! CAAGCTTTAACCTTCTGATT+TGG - tig0033396:17704-17723 None:intergenic 35.0%
AAGGTTAAAGCTTGAGTACA+AGG + tig0033396:17710-17729 Msa1451330:exon 35.0%
AGACACGGAAATAGTATTGC+TGG - tig0033396:16098-16117 None:intergenic 40.0%
! ATTTGGAGTGAGAAAGTGAG+AGG - tig0033396:16129-16148 None:intergenic 40.0%
CCGTATATGAGAAACTTGAG+GGG - tig0033396:16191-16210 None:intergenic 40.0%
CCCCTCAAGTTTCTCATATA+CGG + tig0033396:16188-16207 Msa1451330:CDS 40.0%
GCCGTATATGAGAAACTTGA+GGG - tig0033396:16192-16211 None:intergenic 40.0%
GTATTCGAATTGGATCCCTT+GGG - tig0033396:16258-16277 None:intergenic 40.0%
CAAGGGATCCAATTCGAATA+CGG + tig0033396:16257-16276 Msa1451330:CDS 40.0%
AAGGGATCCAATTCGAATAC+GGG + tig0033396:16258-16277 Msa1451330:CDS 40.0%
! GTTTAATGCTGCTGGTGTTA+CGG + tig0033396:16346-16365 Msa1451330:CDS 40.0%
!! TTTAATGCTGCTGGTGTTAC+GGG + tig0033396:16347-16366 Msa1451330:CDS 40.0%
TGAAACTATCCGTGCTAATG+TGG + tig0033396:16406-16425 Msa1451330:CDS 40.0%
GATCTTCATCTTCTTGGATC+CGG + tig0033396:16515-16534 Msa1451330:CDS 40.0%
ATCTTCATCTTCTTGGATCC+GGG + tig0033396:16516-16535 Msa1451330:CDS 40.0%
AAAGATCACCCGATATGAGA+AGG + tig0033396:17370-17389 Msa1451330:CDS 40.0%
TCTTCCCATCTCAATTGAGA+TGG + tig0033396:17433-17452 Msa1451330:CDS 40.0%
CTTTGCCATCTCAATTGAGA+TGG - tig0033396:17441-17460 None:intergenic 40.0%
! ATCTGGAACTTCACAAATCC+TGG + tig0033396:17476-17495 Msa1451330:CDS 40.0%
!! ATATCGATTCAAGCTTCACC+TGG + tig0033396:17540-17559 Msa1451330:CDS 40.0%
AGTAACAATGAGCTTGATGC+GGG + tig0033396:17611-17630 Msa1451330:CDS 40.0%
TGATGCGGGAAAATTGAAGA+AGG + tig0033396:17625-17644 Msa1451330:CDS 40.0%
TGTCTTCAAGCCAAATCAGA+AGG + tig0033396:17691-17710 Msa1451330:CDS 40.0%
TGAATCATATGAACGGAGAG+AGG - tig0033396:17807-17826 None:intergenic 40.0%
!! CAGGCACTGAATCATATGAA+CGG - tig0033396:17814-17833 None:intergenic 40.0%
! AGAGCTTTTGCGGATTTCTA+TGG + tig0033396:17939-17958 Msa1451330:three_prime_UTR 40.0%
! GAGCTTTTGCGGATTTCTAT+GGG + tig0033396:17940-17959 Msa1451330:three_prime_UTR 40.0%
AGACACGGAAATAGTATTGC+TGG - tig0033396:16098-16117 None:intergenic 40.0%
! ATTTGGAGTGAGAAAGTGAG+AGG - tig0033396:16129-16148 None:intergenic 40.0%
CCGTATATGAGAAACTTGAG+GGG - tig0033396:16191-16210 None:intergenic 40.0%
CCCCTCAAGTTTCTCATATA+CGG + tig0033396:16188-16207 Msa1451330:CDS 40.0%
GCCGTATATGAGAAACTTGA+GGG - tig0033396:16192-16211 None:intergenic 40.0%
GTATTCGAATTGGATCCCTT+GGG - tig0033396:16258-16277 None:intergenic 40.0%
CAAGGGATCCAATTCGAATA+CGG + tig0033396:16257-16276 Msa1451330:CDS 40.0%
AAGGGATCCAATTCGAATAC+GGG + tig0033396:16258-16277 Msa1451330:CDS 40.0%
! GTTTAATGCTGCTGGTGTTA+CGG + tig0033396:16346-16365 Msa1451330:CDS 40.0%
!! TTTAATGCTGCTGGTGTTAC+GGG + tig0033396:16347-16366 Msa1451330:CDS 40.0%
TGAAACTATCCGTGCTAATG+TGG + tig0033396:16406-16425 Msa1451330:CDS 40.0%
GATCTTCATCTTCTTGGATC+CGG + tig0033396:16515-16534 Msa1451330:CDS 40.0%
ATCTTCATCTTCTTGGATCC+GGG + tig0033396:16516-16535 Msa1451330:CDS 40.0%
AAAGATCACCCGATATGAGA+AGG + tig0033396:17370-17389 Msa1451330:CDS 40.0%
TCTTCCCATCTCAATTGAGA+TGG + tig0033396:17433-17452 Msa1451330:CDS 40.0%
CTTTGCCATCTCAATTGAGA+TGG - tig0033396:17441-17460 None:intergenic 40.0%
! ATCTGGAACTTCACAAATCC+TGG + tig0033396:17476-17495 Msa1451330:CDS 40.0%
!! ATATCGATTCAAGCTTCACC+TGG + tig0033396:17540-17559 Msa1451330:CDS 40.0%
AGTAACAATGAGCTTGATGC+GGG + tig0033396:17611-17630 Msa1451330:CDS 40.0%
TGATGCGGGAAAATTGAAGA+AGG + tig0033396:17625-17644 Msa1451330:CDS 40.0%
TGTCTTCAAGCCAAATCAGA+AGG + tig0033396:17691-17710 Msa1451330:CDS 40.0%
TGAATCATATGAACGGAGAG+AGG - tig0033396:17807-17826 None:intergenic 40.0%
!! CAGGCACTGAATCATATGAA+CGG - tig0033396:17814-17833 None:intergenic 40.0%
! AGAGCTTTTGCGGATTTCTA+TGG + tig0033396:17939-17958 Msa1451330:three_prime_UTR 40.0%
! GAGCTTTTGCGGATTTCTAT+GGG + tig0033396:17940-17959 Msa1451330:three_prime_UTR 40.0%
TCAATCGATATGGAGCACGA+TGG - tig0033396:15981-16000 None:intergenic 45.0%
GCTGGGAAACCCATGATATT+TGG - tig0033396:16146-16165 None:intergenic 45.0%
GGCCGTATATGAGAAACTTG+AGG - tig0033396:16193-16212 None:intergenic 45.0%
CGTATTCGAATTGGATCCCT+TGG - tig0033396:16259-16278 None:intergenic 45.0%
GATCCAATTCGAATACGGGT+CGG + tig0033396:16262-16281 Msa1451330:CDS 45.0%
ATCCAATTCGAATACGGGTC+GGG + tig0033396:16263-16282 Msa1451330:CDS 45.0%
GCAGCATTAAACACGTGTGT+TGG - tig0033396:16338-16357 None:intergenic 45.0%
ACACACGTGTTTAATGCTGC+TGG + tig0033396:16338-16357 Msa1451330:CDS 45.0%
ACTATCCGTGCTAATGTGGT+TGG + tig0033396:16410-16429 Msa1451330:CDS 45.0%
CTATCCGTGCTAATGTGGTT+GGG + tig0033396:16411-16430 Msa1451330:CDS 45.0%
!!! TGGATCCGGGATTGGTTTTA+AGG + tig0033396:16529-16548 Msa1451330:CDS 45.0%
TTACTCCAAGACCAAGGCTA+TGG + tig0033396:16583-16602 Msa1451330:CDS 45.0%
! ACATACCATAGCCTTGGTCT+TGG - tig0033396:16591-16610 None:intergenic 45.0%
! TGCAGTAGTTGGAGTATCCT+TGG + tig0033396:16926-16945 Msa1451330:intron 45.0%
! GTTGGAGTATCCTTGGTTGT+CGG + tig0033396:16933-16952 Msa1451330:intron 45.0%
TGATCTTGATCCGACAACCA+AGG - tig0033396:16946-16965 None:intergenic 45.0%
ATGTATGCACCTTGAGAGTG+AGG + tig0033396:17303-17322 Msa1451330:CDS 45.0%
AATAGGCATCCTCACTCTCA+AGG - tig0033396:17315-17334 None:intergenic 45.0%
TGTGATGAAATTGCGAGGGT+TGG - tig0033396:17351-17370 None:intergenic 45.0%
ATGGCAAAGAGAAACCTCAC+TGG + tig0033396:17452-17471 Msa1451330:CDS 45.0%
AGAGAAACCTCACTGGAATC+TGG + tig0033396:17459-17478 Msa1451330:CDS 45.0%
TGAAGTTCCAGATTCCAGTG+AGG - tig0033396:17469-17488 None:intergenic 45.0%
TCATTGTGGCTAACAACTCC+AGG - tig0033396:17497-17516 None:intergenic 45.0%
CTCAAGAGTGAAGTTCTTCC+AGG - tig0033396:17561-17580 None:intergenic 45.0%
CTGGAAGAACTTCACTCTTG+AGG + tig0033396:17559-17578 Msa1451330:CDS 45.0%
CACTCTTGAGGAACAAGCTA+AGG + tig0033396:17571-17590 Msa1451330:CDS 45.0%
! AGTTTGAGCGAGAGCTTTTG+CGG + tig0033396:17929-17948 Msa1451330:three_prime_UTR 45.0%
GGAGTGAACAATGATTCAGC+AGG + tig0033396:17961-17980 Msa1451330:three_prime_UTR 45.0%
TGATTCAGCAGGATCAATGC+AGG + tig0033396:17972-17991 Msa1451330:three_prime_UTR 45.0%
TCAATCGATATGGAGCACGA+TGG - tig0033396:15981-16000 None:intergenic 45.0%
GCTGGGAAACCCATGATATT+TGG - tig0033396:16146-16165 None:intergenic 45.0%
GGCCGTATATGAGAAACTTG+AGG - tig0033396:16193-16212 None:intergenic 45.0%
CGTATTCGAATTGGATCCCT+TGG - tig0033396:16259-16278 None:intergenic 45.0%
GATCCAATTCGAATACGGGT+CGG + tig0033396:16262-16281 Msa1451330:CDS 45.0%
ATCCAATTCGAATACGGGTC+GGG + tig0033396:16263-16282 Msa1451330:CDS 45.0%
GCAGCATTAAACACGTGTGT+TGG - tig0033396:16338-16357 None:intergenic 45.0%
ACACACGTGTTTAATGCTGC+TGG + tig0033396:16338-16357 Msa1451330:CDS 45.0%
ACTATCCGTGCTAATGTGGT+TGG + tig0033396:16410-16429 Msa1451330:CDS 45.0%
CTATCCGTGCTAATGTGGTT+GGG + tig0033396:16411-16430 Msa1451330:CDS 45.0%
!!! TGGATCCGGGATTGGTTTTA+AGG + tig0033396:16529-16548 Msa1451330:CDS 45.0%
TTACTCCAAGACCAAGGCTA+TGG + tig0033396:16583-16602 Msa1451330:CDS 45.0%
! ACATACCATAGCCTTGGTCT+TGG - tig0033396:16591-16610 None:intergenic 45.0%
! TGCAGTAGTTGGAGTATCCT+TGG + tig0033396:16926-16945 Msa1451330:intron 45.0%
! GTTGGAGTATCCTTGGTTGT+CGG + tig0033396:16933-16952 Msa1451330:intron 45.0%
TGATCTTGATCCGACAACCA+AGG - tig0033396:16946-16965 None:intergenic 45.0%
ATGTATGCACCTTGAGAGTG+AGG + tig0033396:17303-17322 Msa1451330:CDS 45.0%
AATAGGCATCCTCACTCTCA+AGG - tig0033396:17315-17334 None:intergenic 45.0%
TGTGATGAAATTGCGAGGGT+TGG - tig0033396:17351-17370 None:intergenic 45.0%
ATGGCAAAGAGAAACCTCAC+TGG + tig0033396:17452-17471 Msa1451330:CDS 45.0%
AGAGAAACCTCACTGGAATC+TGG + tig0033396:17459-17478 Msa1451330:CDS 45.0%
TGAAGTTCCAGATTCCAGTG+AGG - tig0033396:17469-17488 None:intergenic 45.0%
TCATTGTGGCTAACAACTCC+AGG - tig0033396:17497-17516 None:intergenic 45.0%
CTCAAGAGTGAAGTTCTTCC+AGG - tig0033396:17561-17580 None:intergenic 45.0%
CTGGAAGAACTTCACTCTTG+AGG + tig0033396:17559-17578 Msa1451330:CDS 45.0%
CACTCTTGAGGAACAAGCTA+AGG + tig0033396:17571-17590 Msa1451330:CDS 45.0%
! AGTTTGAGCGAGAGCTTTTG+CGG + tig0033396:17929-17948 Msa1451330:three_prime_UTR 45.0%
GGAGTGAACAATGATTCAGC+AGG + tig0033396:17961-17980 Msa1451330:three_prime_UTR 45.0%
TGATTCAGCAGGATCAATGC+AGG + tig0033396:17972-17991 Msa1451330:three_prime_UTR 45.0%
AATACGCATCGAGAGAGCAG+AGG + tig0033396:16061-16080 Msa1451330:five_prime_UTR 50.0%
TGAGAGGAGAGAGAGAGACA+CGG - tig0033396:16113-16132 None:intergenic 50.0%
! GAGAAACTTGAGGGGAGTGT+CGG - tig0033396:16183-16202 None:intergenic 50.0%
TTTCTCATATACGGCCGCAC+CGG + tig0033396:16197-16216 Msa1451330:CDS 50.0%
CGGTACAGAGTTTGCCTAAG+AGG - tig0033396:16235-16254 None:intergenic 50.0%
TTCGAATTGGATCCCTTGGG+CGG - tig0033396:16255-16274 None:intergenic 50.0%
GAGTCCCAACCACATTAGCA+CGG - tig0033396:16418-16437 None:intergenic 50.0%
CATCTTCTTGGATCCGGGAT+TGG + tig0033396:16521-16540 Msa1451330:CDS 50.0%
AATACGCATCGAGAGAGCAG+AGG + tig0033396:16061-16080 Msa1451330:five_prime_UTR 50.0%
TGAGAGGAGAGAGAGAGACA+CGG - tig0033396:16113-16132 None:intergenic 50.0%
! GAGAAACTTGAGGGGAGTGT+CGG - tig0033396:16183-16202 None:intergenic 50.0%
TTTCTCATATACGGCCGCAC+CGG + tig0033396:16197-16216 Msa1451330:CDS 50.0%
CGGTACAGAGTTTGCCTAAG+AGG - tig0033396:16235-16254 None:intergenic 50.0%
TTCGAATTGGATCCCTTGGG+CGG - tig0033396:16255-16274 None:intergenic 50.0%
GAGTCCCAACCACATTAGCA+CGG - tig0033396:16418-16437 None:intergenic 50.0%
CATCTTCTTGGATCCGGGAT+TGG + tig0033396:16521-16540 Msa1451330:CDS 50.0%
!! AAACTTGAGGGGAGTGTCGG+AGG - tig0033396:16180-16199 None:intergenic 55.0%
TAAGAGGCCTCCTATCCAGC+CGG - tig0033396:16219-16238 None:intergenic 55.0%
GCAAACTCTGTACCGCCCAA+GGG + tig0033396:16240-16259 Msa1451330:CDS 55.0%
GACCCGACCCGTATTCGAAT+TGG - tig0033396:16268-16287 None:intergenic 55.0%
GTCTCACACCAGTCCACGTT+GGG - tig0033396:16377-16396 None:intergenic 55.0%
AGTCTCACACCAGTCCACGT+TGG - tig0033396:16378-16397 None:intergenic 55.0%
CACAGGTAGAGGATCTGCTG+AGG + tig0033396:17270-17289 Msa1451330:intron 55.0%
!! AAACTTGAGGGGAGTGTCGG+AGG - tig0033396:16180-16199 None:intergenic 55.0%
TAAGAGGCCTCCTATCCAGC+CGG - tig0033396:16219-16238 None:intergenic 55.0%
GCAAACTCTGTACCGCCCAA+GGG + tig0033396:16240-16259 Msa1451330:CDS 55.0%
GACCCGACCCGTATTCGAAT+TGG - tig0033396:16268-16287 None:intergenic 55.0%
GTCTCACACCAGTCCACGTT+GGG - tig0033396:16377-16396 None:intergenic 55.0%
AGTCTCACACCAGTCCACGT+TGG - tig0033396:16378-16397 None:intergenic 55.0%
CACAGGTAGAGGATCTGCTG+AGG + tig0033396:17270-17289 Msa1451330:intron 55.0%
GGCTGGATAGGAGGCCTCTT+AGG + tig0033396:16218-16237 Msa1451330:CDS 60.0%
GGCAAACTCTGTACCGCCCA+AGG + tig0033396:16239-16258 Msa1451330:CDS 60.0%
TGTTACGGGTCGACCCAACG+TGG + tig0033396:16361-16380 Msa1451330:CDS 60.0%
GGCTGGATAGGAGGCCTCTT+AGG + tig0033396:16218-16237 Msa1451330:CDS 60.0%
GGCAAACTCTGTACCGCCCA+AGG + tig0033396:16239-16258 Msa1451330:CDS 60.0%
TGTTACGGGTCGACCCAACG+TGG + tig0033396:16361-16380 Msa1451330:CDS 60.0%
CGGAGGAGGAGGATGGTGCT+GGG - tig0033396:16163-16182 None:intergenic 65.0%
TCGGAGGAGGAGGATGGTGC+TGG - tig0033396:16164-16183 None:intergenic 65.0%
!! GGAGTGTCGGAGGAGGAGGA+TGG - tig0033396:16170-16189 None:intergenic 65.0%
!! CTTGAGGGGAGTGTCGGAGG+AGG - tig0033396:16177-16196 None:intergenic 65.0%
! TCATATACGGCCGCACCGGC+TGG + tig0033396:16201-16220 Msa1451330:CDS 65.0%
GCTGACGTGTGCCGTGAACG+TGG + tig0033396:16446-16465 Msa1451330:CDS 65.0%
CGGAGGAGGAGGATGGTGCT+GGG - tig0033396:16163-16182 None:intergenic 65.0%
TCGGAGGAGGAGGATGGTGC+TGG - tig0033396:16164-16183 None:intergenic 65.0%
!! GGAGTGTCGGAGGAGGAGGA+TGG - tig0033396:16170-16189 None:intergenic 65.0%
!! CTTGAGGGGAGTGTCGGAGG+AGG - tig0033396:16177-16196 None:intergenic 65.0%
! TCATATACGGCCGCACCGGC+TGG + tig0033396:16201-16220 Msa1451330:CDS 65.0%
GCTGACGTGTGCCGTGAACG+TGG + tig0033396:16446-16465 Msa1451330:CDS 65.0%
!! GAGGGGAGTGTCGGAGGAGG+AGG - tig0033396:16174-16193 None:intergenic 70.0%
!! TACGGCCGCACCGGCTGGAT+AGG + tig0033396:16206-16225 Msa1451330:CDS 70.0%
GGCCTCCTATCCAGCCGGTG+CGG - tig0033396:16214-16233 None:intergenic 70.0%
CGGGTCGACCCAACGTGGAC+TGG + tig0033396:16366-16385 Msa1451330:CDS 70.0%
!! GAGGGGAGTGTCGGAGGAGG+AGG - tig0033396:16174-16193 None:intergenic 70.0%
!! TACGGCCGCACCGGCTGGAT+AGG + tig0033396:16206-16225 Msa1451330:CDS 70.0%
GGCCTCCTATCCAGCCGGTG+CGG - tig0033396:16214-16233 None:intergenic 70.0%
CGGGTCGACCCAACGTGGAC+TGG + tig0033396:16366-16385 Msa1451330:CDS 70.0%
!! GGCCGCACCGGCTGGATAGG+AGG + tig0033396:16209-16228 Msa1451330:CDS 75.0%
!! GGCCGCACCGGCTGGATAGG+AGG + tig0033396:16209-16228 Msa1451330:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0033396 gene 15971 18018 15971 ID=Msa1451330;Name=Msa1451330
tig0033396 mRNA 15971 18018 15971 ID=Msa1451330-mRNA-1;Parent=Msa1451330;Name=Msa1451330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=181|1|1|1|1|1|2|294|300
tig0033396 exon 15971 16604 15971 ID=Msa1451330-mRNA-1:exon:17967;Parent=Msa1451330-mRNA-1
tig0033396 exon 17275 18018 17275 ID=Msa1451330-mRNA-1:exon:17968;Parent=Msa1451330-mRNA-1
tig0033396 five_prime_UTR 15971 16151 15971 ID=Msa1451330-mRNA-1:five_prime_utr;Parent=Msa1451330-mRNA-1
tig0033396 CDS 16152 16604 16152 ID=Msa1451330-mRNA-1:cds;Parent=Msa1451330-mRNA-1
tig0033396 CDS 17275 17724 17275 ID=Msa1451330-mRNA-1:cds;Parent=Msa1451330-mRNA-1
tig0033396 three_prime_UTR 17725 18018 17725 ID=Msa1451330-mRNA-1:three_prime_utr;Parent=Msa1451330-mRNA-1
Gene Sequence

>Msa1451330

ATGGGTTTCCCAGCACCATCCTCCTCCTCCGACACTCCCCTCAAGTTTCTCATATACGGCCGCACCGGCTGGATAGGAGGCCTCTTAGGCAAACTCTGTACCGCCCAAGGGATCCAATTCGAATACGGGTCGGGTCGTCTTGAGAATCGATCTTCATTGGAATCTGACATAGCTGAGATTAAACCAACACACGTGTTTAATGCTGCTGGTGTTACGGGTCGACCCAACGTGGACTGGTGTGAGACTCATAAAGTTGAAACTATCCGTGCTAATGTGGTTGGGACTCTCACTCTTGCTGACGTGTGCCGTGAACGTGGACTTATTGTTATTAATTTTGCTACTGGATGTATTTTTGAGTATGATGATCTTCATCTTCTTGGATCCGGGATTGGTTTTAAGGAAGAAGATACTCCTAATTTCATTGGTTCTTTTTACTCCAAGACCAAGGCTATGGTAGAGGATCTGCTGAGGAACTACGATAATGTATGCACCTTGAGAGTGAGGATGCCTATTTCATCTGATTTATCCAACCCTCGCAATTTCATCACAAAGATCACCCGATATGAGAAGGTTGTAGATATTCCAAACTCGATGACCATTTTAGATGAACTTCTTCCCATCTCAATTGAGATGGCAAAGAGAAACCTCACTGGAATCTGGAACTTCACAAATCCTGGAGTTGTTAGCCACAATGAAATCTTACAAATGTACAAAGACTATATCGATTCAAGCTTCACCTGGAAGAACTTCACTCTTGAGGAACAAGCTAAGGTGATAGTTGCCCCAAGAAGTAACAATGAGCTTGATGCGGGAAAATTGAAGAAGGAATTTCCAGAACTATTGTCTATCAAGGAATCACTCATTGAGAATGTCTTCAAGCCAAATCAGAAGGTTAAAGCTTGA
Protein sequence

>Msa1451330

MGFPAPSSSSDTPLKFLIYGRTGWIGGLLGKLCTAQGIQFEYGSGRLENRSSLESDIAEIKPTHVFNAAGVTGRPNVDWCETHKVETIRANVVGTLTLADVCRERGLIVINFATGCIFEYDDLHLLGSGIGFKEEDTPNFIGSFYSKTKAMVEDLLRNYDNVCTLRVRMPISSDLSNPRNFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILQMYKDYIDSSFTWKNFTLEEQAKVIVAPRSNNELDAGKLKKEFPELLSIKESLIENVFKPNQKVKA*