AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1447400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1447400 MtrunA17_Chr4g0057161 98.000 300 4 1 1 300 1 298 0.0 603
Msa1447400 MtrunA17_Chr8g0359401 82.807 285 49 0 13 297 383 667 2.81e-177 505
Msa1447400 MtrunA17_Chr2g0314601 81.724 290 53 0 8 297 379 668 5.49e-176 502
Msa1447400 MtrunA17_Chr1g0147841 78.873 284 60 0 14 297 371 654 2.82e-167 479
Msa1447400 MtrunA17_Chr3g0097081 61.616 99 38 0 7 105 160 258 9.20e-33 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1447400 AT1G63000.1 83.566 286 47 0 14 299 13 298 0.0 512
Msa1447400 AT1G78570.1 80.808 297 52 2 6 297 372 668 9.79e-177 504
Msa1447400 AT1G53500.1 78.621 290 62 0 8 297 377 666 1.47e-157 455
Msa1447400 AT3G14790.1 78.157 293 64 0 5 297 371 663 2.26e-157 454
Msa1447400 AT3G14790.2 78.157 293 64 0 5 297 374 666 2.76e-157 454

Find 103 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATGATCTTCATCTTCT+TGG 0.210029 tig0031748:+16889 Msa1447400:CDS
TCTAAAATGGTCATCGAGTT+TGG 0.262726 tig0031748:-17783 None:intergenic
TCAAGCTCATTGTTACTTCT+TGG 0.275443 tig0031748:-17984 None:intergenic
GCTGGGAAACCCATGATATT+TGG 0.303776 tig0031748:-16523 None:intergenic
CAAGCTCATTGTTACTTCTT+GGG 0.312171 tig0031748:-17983 None:intergenic
CAAGCTTTAACCTTCTGATT+TGG 0.317551 tig0031748:-18080 None:intergenic
GGGAAGAAGTTCATCTAAAA+TGG 0.318463 tig0031748:-17796 None:intergenic
CATCTTCTTGGATCCGGGAT+TGG 0.324588 tig0031748:+16901 Msa1447400:CDS
CCCCTCAAGTTTCTCATATA+CGG 0.329932 tig0031748:+16568 Msa1447400:CDS
TTTAATGCTGCTGGTGTTAC+GGG 0.336811 tig0031748:+16727 Msa1447400:CDS
CTCAAGAGTGAAGTTCTTCC+AGG 0.342280 tig0031748:-17937 None:intergenic
GAGAATCAAATCAATCGATA+TGG 0.362687 tig0031748:-16368 None:intergenic
AGAGAAACCTCACTGGAATC+TGG 0.362925 tig0031748:+17838 Msa1447400:CDS
TTGGATAAATCAGATGAAAT+AGG 0.363426 tig0031748:-17708 None:intergenic
TCCTTGATAGACAATAGTTC+TGG 0.368427 tig0031748:-18032 None:intergenic
GAAGATACTCCTAATTTCAT+TGG 0.368560 tig0031748:+16934 Msa1447400:CDS
TCTTGAGAATCGATCTTCAT+TGG 0.371797 tig0031748:+16669 Msa1447400:CDS
TATCTACAACCTTCTCATAT+CGG 0.390642 tig0031748:-17758 None:intergenic
GTTTAATGCTGCTGGTGTTA+CGG 0.392378 tig0031748:+16726 Msa1447400:CDS
CTTTCTCACTCCAAATATCA+TGG 0.393877 tig0031748:+16513 Msa1447400:exon
GATCTTCATCTTCTTGGATC+CGG 0.397535 tig0031748:+16895 Msa1447400:CDS
TTCTTTATAACGTAAATAAA+CGG 0.400489 tig0031748:-16390 None:intergenic
CGTATTCGAATTGGATCCCT+TGG 0.400642 tig0031748:-16636 None:intergenic
GACCCGACCCGTATTCGAAT+TGG 0.406229 tig0031748:-16645 None:intergenic
TTTATTTACGTTATAAAGAA+AGG 0.407222 tig0031748:+16393 Msa1447400:five_prime_UTR
TCGGAGGAGGAGGATGGTGC+TGG 0.407640 tig0031748:-16541 None:intergenic
ATCTTCATCTTCTTGGATCC+GGG 0.408563 tig0031748:+16896 Msa1447400:CDS
ATCTGGAACTTCACAAATCC+TGG 0.419713 tig0031748:+17855 Msa1447400:CDS
CTTTGCCATCTCAATTGAGA+TGG 0.421086 tig0031748:-17817 None:intergenic
TCCAGAACTATTGTCTATCA+AGG 0.428839 tig0031748:+18031 Msa1447400:CDS
GGCTGGATAGGAGGCCTCTT+AGG 0.432855 tig0031748:+16598 Msa1447400:CDS
CAAGGGATCCAATTCGAATA+CGG 0.434923 tig0031748:+16637 Msa1447400:CDS
GAGGGGAGTGTCGGAGGAGG+AGG 0.437559 tig0031748:-16551 None:intergenic
GAAAATAATACCCCATATAC+AGG 0.441120 tig0031748:-18386 None:intergenic
GTCTCACACCAGTCCACGTT+GGG 0.462588 tig0031748:-16754 None:intergenic
ATCTACAACCTTCTCATATC+GGG 0.463578 tig0031748:-17757 None:intergenic
TTCTTCCTTAAAACCAATCC+CGG 0.465949 tig0031748:-16914 None:intergenic
TCATATACGGCCGCACCGGC+TGG 0.469750 tig0031748:+16581 Msa1447400:CDS
TCATTGTGGCTAACAACTCC+AGG 0.479957 tig0031748:-17873 None:intergenic
ACATACCATAGCCTTGGTCT+TGG 0.487323 tig0031748:-16968 None:intergenic
AGACACGGAAATAGTATTGC+TGG 0.489889 tig0031748:-16475 None:intergenic
AAGCTCATTGTTACTTCTTG+GGG 0.490161 tig0031748:-17982 None:intergenic
CGGTACAGAGTTTGCCTAAG+AGG 0.492780 tig0031748:-16612 None:intergenic
CGGGTCGACCCAACGTGGAC+TGG 0.494677 tig0031748:+16746 Msa1447400:CDS
TAACAATAAGTCCACGTTCA+CGG 0.500654 tig0031748:-16837 None:intergenic
CTATCCGTGCTAATGTGGTT+GGG 0.501072 tig0031748:+16791 Msa1447400:CDS
ATCCAATTCGAATACGGGTC+GGG 0.505835 tig0031748:+16643 Msa1447400:CDS
AAGGTTAAAGCTTGAGTACA+AGG 0.509109 tig0031748:+18089 Msa1447400:exon
GGCAAACTCTGTACCGCCCA+AGG 0.511999 tig0031748:+16619 Msa1447400:CDS
TTACTCCAAGACCAAGGCTA+TGG 0.515471 tig0031748:+16963 Msa1447400:CDS
GCCGTATATGAGAAACTTGA+GGG 0.516441 tig0031748:-16569 None:intergenic
CTTGAGGGGAGTGTCGGAGG+AGG 0.523463 tig0031748:-16554 None:intergenic
CACTCTTGAGGAACAAGCTA+AGG 0.523587 tig0031748:+17950 Msa1447400:CDS
CGGAGGAGGAGGATGGTGCT+GGG 0.526896 tig0031748:-16540 None:intergenic
TTTCTCACTCCAAATATCAT+GGG 0.527605 tig0031748:+16514 Msa1447400:exon
AGTAACAATGAGCTTGATGC+GGG 0.528741 tig0031748:+17990 Msa1447400:CDS
GTATTCGAATTGGATCCCTT+GGG 0.529711 tig0031748:-16635 None:intergenic
TTGATTTATTTCAGTTTGTG+TGG 0.531764 tig0031748:+18144 Msa1447400:three_prime_UTR
TTTGCCATCTCAATTGAGAT+GGG 0.537632 tig0031748:-17816 None:intergenic
AAGGGATCCAATTCGAATAC+GGG 0.542692 tig0031748:+16638 Msa1447400:CDS
TGTGATGAAATTGCGAGGGT+TGG 0.546615 tig0031748:-17727 None:intergenic
ATTTGGAGTGAGAAAGTGAG+AGG 0.546724 tig0031748:-16506 None:intergenic
AATGTAACATACCATAGCCT+TGG 0.554984 tig0031748:-16974 None:intergenic
TTTCTCATATACGGCCGCAC+CGG 0.558062 tig0031748:+16577 Msa1447400:CDS
TCTTCCCATCTCAATTGAGA+TGG 0.561970 tig0031748:+17812 Msa1447400:CDS
ATATCGATTCAAGCTTCACC+TGG 0.568911 tig0031748:+17919 Msa1447400:CDS
TAAGAGGCCTCCTATCCAGC+CGG 0.572784 tig0031748:-16596 None:intergenic
GGAGTGTCGGAGGAGGAGGA+TGG 0.576494 tig0031748:-16547 None:intergenic
AAACTTGAGGGGAGTGTCGG+AGG 0.580469 tig0031748:-16557 None:intergenic
ACTATCCGTGCTAATGTGGT+TGG 0.582347 tig0031748:+16790 Msa1447400:CDS
GCAGCATTAAACACGTGTGT+TGG 0.584742 tig0031748:-16715 None:intergenic
TGATTCAGCAGGATCAATGC+AGG 0.585648 tig0031748:+18349 Msa1447400:three_prime_UTR
TACGGCCGCACCGGCTGGAT+AGG 0.586868 tig0031748:+16586 Msa1447400:CDS
GAGAAACTTGAGGGGAGTGT+CGG 0.586872 tig0031748:-16560 None:intergenic
CTGGAAGAACTTCACTCTTG+AGG 0.589081 tig0031748:+17938 Msa1447400:CDS
GGCCGTATATGAGAAACTTG+AGG 0.591913 tig0031748:-16570 None:intergenic
AATAGGCATCCTCACTCTCA+AGG 0.595727 tig0031748:-17691 None:intergenic
TGATGCGGGAAAATTGAAGA+AGG 0.598610 tig0031748:+18004 Msa1447400:CDS
TGTCTTCAAGCCAAATCAGA+AGG 0.603264 tig0031748:+18070 Msa1447400:CDS
ATGGCAAAGAGAAACCTCAC+TGG 0.606695 tig0031748:+17831 Msa1447400:CDS
GGAGTGAACAATGATTCAGC+AGG 0.617978 tig0031748:+18338 Msa1447400:three_prime_UTR
AAAGATCACCCGATATGAGA+AGG 0.619965 tig0031748:+17749 Msa1447400:CDS
TTCGAATTGGATCCCTTGGG+CGG 0.621824 tig0031748:-16632 None:intergenic
GCAAACTCTGTACCGCCCAA+GGG 0.622614 tig0031748:+16620 Msa1447400:CDS
ACACACGTGTTTAATGCTGC+TGG 0.626893 tig0031748:+16718 Msa1447400:CDS
ATCTTTGTGATGAAATTGCG+AGG 0.632297 tig0031748:-17732 None:intergenic
GGCCTCCTATCCAGCCGGTG+CGG 0.634921 tig0031748:-16591 None:intergenic
GATCCAATTCGAATACGGGT+CGG 0.635111 tig0031748:+16642 Msa1447400:CDS
AAGTAACAATGAGCTTGATG+CGG 0.635352 tig0031748:+17989 Msa1447400:CDS
ATGTATGCACCTTGAGAGTG+AGG 0.639312 tig0031748:+17682 Msa1447400:CDS
TGAAACTATCCGTGCTAATG+TGG 0.665772 tig0031748:+16786 Msa1447400:CDS
CAGGCACTGAATCATATGAA+CGG 0.668390 tig0031748:-18188 None:intergenic
GAGTCCCAACCACATTAGCA+CGG 0.686328 tig0031748:-16795 None:intergenic
ACATTTGTAAGATTTCATTG+TGG 0.688248 tig0031748:-17887 None:intergenic
AGTCTCACACCAGTCCACGT+TGG 0.693140 tig0031748:-16755 None:intergenic
AATACGCATCGAGAGAGCAG+AGG 0.696381 tig0031748:+16441 Msa1447400:five_prime_UTR
CACAGGTAGAGGATCTGCTG+AGG 0.704068 tig0031748:+17649 Msa1447400:intron
TGTTACGGGTCGACCCAACG+TGG 0.704086 tig0031748:+16741 Msa1447400:CDS
TCAATCGATATGGAGCACGA+TGG 0.707527 tig0031748:-16358 None:intergenic
TGAAGTTCCAGATTCCAGTG+AGG 0.720062 tig0031748:-17845 None:intergenic
TCTTTGTGATGAAATTGCGA+GGG 0.720478 tig0031748:-17731 None:intergenic
CCGTATATGAGAAACTTGAG+GGG 0.728448 tig0031748:-16568 None:intergenic
ACTGAATCATATGAACGGAG+AGG 0.733536 tig0031748:-18183 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTTATAACGTAAATAAA+CGG - tig0031748:16393-16412 None:intergenic 15.0%
!! TTTATTTACGTTATAAAGAA+AGG + tig0031748:16393-16412 Msa1447400:five_prime_UTR 15.0%
!! TATAATATAAAACAGATCTA+AGG - tig0031748:17167-17186 None:intergenic 15.0%
!! CAACATATAAATAAAAATAC+AGG - tig0031748:18210-18229 None:intergenic 15.0%
!! TTCTTTATAACGTAAATAAA+CGG - tig0031748:16393-16412 None:intergenic 15.0%
!! TTTATTTACGTTATAAAGAA+AGG + tig0031748:16393-16412 Msa1447400:five_prime_UTR 15.0%
!! TATAATATAAAACAGATCTA+AGG - tig0031748:17167-17186 None:intergenic 15.0%
!! CAACATATAAATAAAAATAC+AGG - tig0031748:18210-18229 None:intergenic 15.0%
!!! ATTGTTATTAATTTTGCTAC+TGG + tig0031748:16853-16872 Msa1447400:CDS 20.0%
!! TAAAAAGAACCAATGAAATT+AGG - tig0031748:16946-16965 None:intergenic 20.0%
!!! TGTTTAAGATTGATGTTTTT+TGG + tig0031748:17022-17041 Msa1447400:intron 20.0%
!! ATATCAAACAAATCAAAACA+CGG - tig0031748:17267-17286 None:intergenic 20.0%
!!! ATTGTTATTAATTTTGCTAC+TGG + tig0031748:16853-16872 Msa1447400:CDS 20.0%
!! TAAAAAGAACCAATGAAATT+AGG - tig0031748:16946-16965 None:intergenic 20.0%
!!! TGTTTAAGATTGATGTTTTT+TGG + tig0031748:17022-17041 Msa1447400:intron 20.0%
!! ATATCAAACAAATCAAAACA+CGG - tig0031748:17267-17286 None:intergenic 20.0%
!! TATGGTATGTTACATTGATT+TGG + tig0031748:16981-17000 Msa1447400:intron 25.0%
!!! ATGCTGATTTGTTGAATTAT+TGG + tig0031748:17095-17114 Msa1447400:intron 25.0%
!!! GATTTGTTGAATTATTGGTT+AGG + tig0031748:17100-17119 Msa1447400:intron 25.0%
!!! TTACTTTTATATGCAGTAGT+TGG + tig0031748:17294-17313 Msa1447400:intron 25.0%
!! CTTAAGAGACTAATGTTTTT+CGG - tig0031748:17496-17515 None:intergenic 25.0%
!! AAAAACATTAGTCTCTTAAG+CGG + tig0031748:17496-17515 Msa1447400:intron 25.0%
! ACATTAACAAAAAGAAAGCA+AGG + tig0031748:17570-17589 Msa1447400:intron 25.0%
! TTGGATAAATCAGATGAAAT+AGG - tig0031748:17711-17730 None:intergenic 25.0%
! ACATTTGTAAGATTTCATTG+TGG - tig0031748:17890-17909 None:intergenic 25.0%
! TTGATTTATTTCAGTTTGTG+TGG + tig0031748:18144-18163 Msa1447400:three_prime_UTR 25.0%
!! TATGGTATGTTACATTGATT+TGG + tig0031748:16981-17000 Msa1447400:intron 25.0%
!!! ATGCTGATTTGTTGAATTAT+TGG + tig0031748:17095-17114 Msa1447400:intron 25.0%
!!! GATTTGTTGAATTATTGGTT+AGG + tig0031748:17100-17119 Msa1447400:intron 25.0%
!!! TTACTTTTATATGCAGTAGT+TGG + tig0031748:17294-17313 Msa1447400:intron 25.0%
!! CTTAAGAGACTAATGTTTTT+CGG - tig0031748:17496-17515 None:intergenic 25.0%
!! AAAAACATTAGTCTCTTAAG+CGG + tig0031748:17496-17515 Msa1447400:intron 25.0%
! ACATTAACAAAAAGAAAGCA+AGG + tig0031748:17570-17589 Msa1447400:intron 25.0%
! TTGGATAAATCAGATGAAAT+AGG - tig0031748:17711-17730 None:intergenic 25.0%
! ACATTTGTAAGATTTCATTG+TGG - tig0031748:17890-17909 None:intergenic 25.0%
! TTGATTTATTTCAGTTTGTG+TGG + tig0031748:18144-18163 Msa1447400:three_prime_UTR 25.0%
GAGAATCAAATCAATCGATA+TGG - tig0031748:16371-16390 None:intergenic 30.0%
TTTCTCACTCCAAATATCAT+GGG + tig0031748:16514-16533 Msa1447400:exon 30.0%
TATGATGATCTTCATCTTCT+TGG + tig0031748:16889-16908 Msa1447400:CDS 30.0%
GAAGATACTCCTAATTTCAT+TGG + tig0031748:16934-16953 Msa1447400:CDS 30.0%
TAGTCATTCGTTTGTAACAT+TGG - tig0031748:17443-17462 None:intergenic 30.0%
TTAACAAAAAGAAAGCAAGG+TGG + tig0031748:17573-17592 Msa1447400:intron 30.0%
GTTCACAAAAAAGGAAAACA+AGG - tig0031748:17630-17649 None:intergenic 30.0%
!! TTTTCCTTTTTTGTGAACAC+AGG + tig0031748:17632-17651 Msa1447400:intron 30.0%
TATCTACAACCTTCTCATAT+CGG - tig0031748:17761-17780 None:intergenic 30.0%
GAGAATCAAATCAATCGATA+TGG - tig0031748:16371-16390 None:intergenic 30.0%
TTTCTCACTCCAAATATCAT+GGG + tig0031748:16514-16533 Msa1447400:exon 30.0%
TATGATGATCTTCATCTTCT+TGG + tig0031748:16889-16908 Msa1447400:CDS 30.0%
GAAGATACTCCTAATTTCAT+TGG + tig0031748:16934-16953 Msa1447400:CDS 30.0%
TAGTCATTCGTTTGTAACAT+TGG - tig0031748:17443-17462 None:intergenic 30.0%
TTAACAAAAAGAAAGCAAGG+TGG + tig0031748:17573-17592 Msa1447400:intron 30.0%
GTTCACAAAAAAGGAAAACA+AGG - tig0031748:17630-17649 None:intergenic 30.0%
!! TTTTCCTTTTTTGTGAACAC+AGG + tig0031748:17632-17651 Msa1447400:intron 30.0%
TATCTACAACCTTCTCATAT+CGG - tig0031748:17761-17780 None:intergenic 30.0%
CTTTCTCACTCCAAATATCA+TGG + tig0031748:16513-16532 Msa1447400:exon 35.0%
TCTTGAGAATCGATCTTCAT+TGG + tig0031748:16669-16688 Msa1447400:CDS 35.0%
TAACAATAAGTCCACGTTCA+CGG - tig0031748:16840-16859 None:intergenic 35.0%
TTCTTCCTTAAAACCAATCC+CGG - tig0031748:16917-16936 None:intergenic 35.0%
! TTCTTTTTACTCCAAGACCA+AGG + tig0031748:16957-16976 Msa1447400:CDS 35.0%
AATGTAACATACCATAGCCT+TGG - tig0031748:16977-16996 None:intergenic 35.0%
TGTTACAAACGAATGACTAC+AGG + tig0031748:17444-17463 Msa1447400:intron 35.0%
GTTACAAACGAATGACTACA+GGG + tig0031748:17445-17464 Msa1447400:intron 35.0%
! AGAAAGCAAGGTGGTATTTT+AGG + tig0031748:17582-17601 Msa1447400:intron 35.0%
TCTACCTGTGTTCACAAAAA+AGG - tig0031748:17639-17658 None:intergenic 35.0%
!!! TTTTTTGTGAACACAGGTAG+AGG + tig0031748:17638-17657 Msa1447400:intron 35.0%
TCTTTGTGATGAAATTGCGA+GGG - tig0031748:17734-17753 None:intergenic 35.0%
ATCTTTGTGATGAAATTGCG+AGG - tig0031748:17735-17754 None:intergenic 35.0%
ATCTACAACCTTCTCATATC+GGG - tig0031748:17760-17779 None:intergenic 35.0%
! TCTAAAATGGTCATCGAGTT+TGG - tig0031748:17786-17805 None:intergenic 35.0%
GGGAAGAAGTTCATCTAAAA+TGG - tig0031748:17799-17818 None:intergenic 35.0%
TTTGCCATCTCAATTGAGAT+GGG - tig0031748:17819-17838 None:intergenic 35.0%
! AAGCTCATTGTTACTTCTTG+GGG - tig0031748:17985-18004 None:intergenic 35.0%
! CAAGCTCATTGTTACTTCTT+GGG - tig0031748:17986-18005 None:intergenic 35.0%
! TCAAGCTCATTGTTACTTCT+TGG - tig0031748:17987-18006 None:intergenic 35.0%
AAGTAACAATGAGCTTGATG+CGG + tig0031748:17989-18008 Msa1447400:CDS 35.0%
TCCAGAACTATTGTCTATCA+AGG + tig0031748:18031-18050 Msa1447400:CDS 35.0%
TCCTTGATAGACAATAGTTC+TGG - tig0031748:18035-18054 None:intergenic 35.0%
! CAAGCTTTAACCTTCTGATT+TGG - tig0031748:18083-18102 None:intergenic 35.0%
AAGGTTAAAGCTTGAGTACA+AGG + tig0031748:18089-18108 Msa1447400:exon 35.0%
CTTTCTCACTCCAAATATCA+TGG + tig0031748:16513-16532 Msa1447400:exon 35.0%
TCTTGAGAATCGATCTTCAT+TGG + tig0031748:16669-16688 Msa1447400:CDS 35.0%
TAACAATAAGTCCACGTTCA+CGG - tig0031748:16840-16859 None:intergenic 35.0%
TTCTTCCTTAAAACCAATCC+CGG - tig0031748:16917-16936 None:intergenic 35.0%
! TTCTTTTTACTCCAAGACCA+AGG + tig0031748:16957-16976 Msa1447400:CDS 35.0%
AATGTAACATACCATAGCCT+TGG - tig0031748:16977-16996 None:intergenic 35.0%
TGTTACAAACGAATGACTAC+AGG + tig0031748:17444-17463 Msa1447400:intron 35.0%
GTTACAAACGAATGACTACA+GGG + tig0031748:17445-17464 Msa1447400:intron 35.0%
! AGAAAGCAAGGTGGTATTTT+AGG + tig0031748:17582-17601 Msa1447400:intron 35.0%
TCTACCTGTGTTCACAAAAA+AGG - tig0031748:17639-17658 None:intergenic 35.0%
!!! TTTTTTGTGAACACAGGTAG+AGG + tig0031748:17638-17657 Msa1447400:intron 35.0%
TCTTTGTGATGAAATTGCGA+GGG - tig0031748:17734-17753 None:intergenic 35.0%
ATCTTTGTGATGAAATTGCG+AGG - tig0031748:17735-17754 None:intergenic 35.0%
ATCTACAACCTTCTCATATC+GGG - tig0031748:17760-17779 None:intergenic 35.0%
! TCTAAAATGGTCATCGAGTT+TGG - tig0031748:17786-17805 None:intergenic 35.0%
GGGAAGAAGTTCATCTAAAA+TGG - tig0031748:17799-17818 None:intergenic 35.0%
TTTGCCATCTCAATTGAGAT+GGG - tig0031748:17819-17838 None:intergenic 35.0%
! AAGCTCATTGTTACTTCTTG+GGG - tig0031748:17985-18004 None:intergenic 35.0%
! CAAGCTCATTGTTACTTCTT+GGG - tig0031748:17986-18005 None:intergenic 35.0%
! TCAAGCTCATTGTTACTTCT+TGG - tig0031748:17987-18006 None:intergenic 35.0%
AAGTAACAATGAGCTTGATG+CGG + tig0031748:17989-18008 Msa1447400:CDS 35.0%
TCCAGAACTATTGTCTATCA+AGG + tig0031748:18031-18050 Msa1447400:CDS 35.0%
TCCTTGATAGACAATAGTTC+TGG - tig0031748:18035-18054 None:intergenic 35.0%
! CAAGCTTTAACCTTCTGATT+TGG - tig0031748:18083-18102 None:intergenic 35.0%
AAGGTTAAAGCTTGAGTACA+AGG + tig0031748:18089-18108 Msa1447400:exon 35.0%
AGACACGGAAATAGTATTGC+TGG - tig0031748:16478-16497 None:intergenic 40.0%
! ATTTGGAGTGAGAAAGTGAG+AGG - tig0031748:16509-16528 None:intergenic 40.0%
CCGTATATGAGAAACTTGAG+GGG - tig0031748:16571-16590 None:intergenic 40.0%
CCCCTCAAGTTTCTCATATA+CGG + tig0031748:16568-16587 Msa1447400:CDS 40.0%
GCCGTATATGAGAAACTTGA+GGG - tig0031748:16572-16591 None:intergenic 40.0%
GTATTCGAATTGGATCCCTT+GGG - tig0031748:16638-16657 None:intergenic 40.0%
CAAGGGATCCAATTCGAATA+CGG + tig0031748:16637-16656 Msa1447400:CDS 40.0%
AAGGGATCCAATTCGAATAC+GGG + tig0031748:16638-16657 Msa1447400:CDS 40.0%
! GTTTAATGCTGCTGGTGTTA+CGG + tig0031748:16726-16745 Msa1447400:CDS 40.0%
!! TTTAATGCTGCTGGTGTTAC+GGG + tig0031748:16727-16746 Msa1447400:CDS 40.0%
TGAAACTATCCGTGCTAATG+TGG + tig0031748:16786-16805 Msa1447400:CDS 40.0%
GATCTTCATCTTCTTGGATC+CGG + tig0031748:16895-16914 Msa1447400:CDS 40.0%
ATCTTCATCTTCTTGGATCC+GGG + tig0031748:16896-16915 Msa1447400:CDS 40.0%
AAAGATCACCCGATATGAGA+AGG + tig0031748:17749-17768 Msa1447400:CDS 40.0%
TCTTCCCATCTCAATTGAGA+TGG + tig0031748:17812-17831 Msa1447400:CDS 40.0%
CTTTGCCATCTCAATTGAGA+TGG - tig0031748:17820-17839 None:intergenic 40.0%
! ATCTGGAACTTCACAAATCC+TGG + tig0031748:17855-17874 Msa1447400:CDS 40.0%
!! ATATCGATTCAAGCTTCACC+TGG + tig0031748:17919-17938 Msa1447400:CDS 40.0%
AGTAACAATGAGCTTGATGC+GGG + tig0031748:17990-18009 Msa1447400:CDS 40.0%
TGATGCGGGAAAATTGAAGA+AGG + tig0031748:18004-18023 Msa1447400:CDS 40.0%
TGTCTTCAAGCCAAATCAGA+AGG + tig0031748:18070-18089 Msa1447400:CDS 40.0%
ACTGAATCATATGAACGGAG+AGG - tig0031748:18186-18205 None:intergenic 40.0%
!! CAGGCACTGAATCATATGAA+CGG - tig0031748:18191-18210 None:intergenic 40.0%
! AGAGCTTTTGCGGATTTCTA+TGG + tig0031748:18316-18335 Msa1447400:three_prime_UTR 40.0%
! GAGCTTTTGCGGATTTCTAT+GGG + tig0031748:18317-18336 Msa1447400:three_prime_UTR 40.0%
AGACACGGAAATAGTATTGC+TGG - tig0031748:16478-16497 None:intergenic 40.0%
! ATTTGGAGTGAGAAAGTGAG+AGG - tig0031748:16509-16528 None:intergenic 40.0%
CCGTATATGAGAAACTTGAG+GGG - tig0031748:16571-16590 None:intergenic 40.0%
CCCCTCAAGTTTCTCATATA+CGG + tig0031748:16568-16587 Msa1447400:CDS 40.0%
GCCGTATATGAGAAACTTGA+GGG - tig0031748:16572-16591 None:intergenic 40.0%
GTATTCGAATTGGATCCCTT+GGG - tig0031748:16638-16657 None:intergenic 40.0%
CAAGGGATCCAATTCGAATA+CGG + tig0031748:16637-16656 Msa1447400:CDS 40.0%
AAGGGATCCAATTCGAATAC+GGG + tig0031748:16638-16657 Msa1447400:CDS 40.0%
! GTTTAATGCTGCTGGTGTTA+CGG + tig0031748:16726-16745 Msa1447400:CDS 40.0%
!! TTTAATGCTGCTGGTGTTAC+GGG + tig0031748:16727-16746 Msa1447400:CDS 40.0%
TGAAACTATCCGTGCTAATG+TGG + tig0031748:16786-16805 Msa1447400:CDS 40.0%
GATCTTCATCTTCTTGGATC+CGG + tig0031748:16895-16914 Msa1447400:CDS 40.0%
ATCTTCATCTTCTTGGATCC+GGG + tig0031748:16896-16915 Msa1447400:CDS 40.0%
AAAGATCACCCGATATGAGA+AGG + tig0031748:17749-17768 Msa1447400:CDS 40.0%
TCTTCCCATCTCAATTGAGA+TGG + tig0031748:17812-17831 Msa1447400:CDS 40.0%
CTTTGCCATCTCAATTGAGA+TGG - tig0031748:17820-17839 None:intergenic 40.0%
! ATCTGGAACTTCACAAATCC+TGG + tig0031748:17855-17874 Msa1447400:CDS 40.0%
!! ATATCGATTCAAGCTTCACC+TGG + tig0031748:17919-17938 Msa1447400:CDS 40.0%
AGTAACAATGAGCTTGATGC+GGG + tig0031748:17990-18009 Msa1447400:CDS 40.0%
TGATGCGGGAAAATTGAAGA+AGG + tig0031748:18004-18023 Msa1447400:CDS 40.0%
TGTCTTCAAGCCAAATCAGA+AGG + tig0031748:18070-18089 Msa1447400:CDS 40.0%
ACTGAATCATATGAACGGAG+AGG - tig0031748:18186-18205 None:intergenic 40.0%
!! CAGGCACTGAATCATATGAA+CGG - tig0031748:18191-18210 None:intergenic 40.0%
! AGAGCTTTTGCGGATTTCTA+TGG + tig0031748:18316-18335 Msa1447400:three_prime_UTR 40.0%
! GAGCTTTTGCGGATTTCTAT+GGG + tig0031748:18317-18336 Msa1447400:three_prime_UTR 40.0%
TCAATCGATATGGAGCACGA+TGG - tig0031748:16361-16380 None:intergenic 45.0%
GCTGGGAAACCCATGATATT+TGG - tig0031748:16526-16545 None:intergenic 45.0%
GGCCGTATATGAGAAACTTG+AGG - tig0031748:16573-16592 None:intergenic 45.0%
CGTATTCGAATTGGATCCCT+TGG - tig0031748:16639-16658 None:intergenic 45.0%
GATCCAATTCGAATACGGGT+CGG + tig0031748:16642-16661 Msa1447400:CDS 45.0%
ATCCAATTCGAATACGGGTC+GGG + tig0031748:16643-16662 Msa1447400:CDS 45.0%
GCAGCATTAAACACGTGTGT+TGG - tig0031748:16718-16737 None:intergenic 45.0%
ACACACGTGTTTAATGCTGC+TGG + tig0031748:16718-16737 Msa1447400:CDS 45.0%
ACTATCCGTGCTAATGTGGT+TGG + tig0031748:16790-16809 Msa1447400:CDS 45.0%
CTATCCGTGCTAATGTGGTT+GGG + tig0031748:16791-16810 Msa1447400:CDS 45.0%
!!! TGGATCCGGGATTGGTTTTA+AGG + tig0031748:16909-16928 Msa1447400:CDS 45.0%
TTACTCCAAGACCAAGGCTA+TGG + tig0031748:16963-16982 Msa1447400:CDS 45.0%
! ACATACCATAGCCTTGGTCT+TGG - tig0031748:16971-16990 None:intergenic 45.0%
! TGCAGTAGTTGGAGTATCCT+TGG + tig0031748:17305-17324 Msa1447400:intron 45.0%
! GTTGGAGTATCCTTGGTTGT+CGG + tig0031748:17312-17331 Msa1447400:intron 45.0%
TGATCTTGATCCGACAACCA+AGG - tig0031748:17325-17344 None:intergenic 45.0%
ATGTATGCACCTTGAGAGTG+AGG + tig0031748:17682-17701 Msa1447400:CDS 45.0%
AATAGGCATCCTCACTCTCA+AGG - tig0031748:17694-17713 None:intergenic 45.0%
TGTGATGAAATTGCGAGGGT+TGG - tig0031748:17730-17749 None:intergenic 45.0%
ATGGCAAAGAGAAACCTCAC+TGG + tig0031748:17831-17850 Msa1447400:CDS 45.0%
AGAGAAACCTCACTGGAATC+TGG + tig0031748:17838-17857 Msa1447400:CDS 45.0%
TGAAGTTCCAGATTCCAGTG+AGG - tig0031748:17848-17867 None:intergenic 45.0%
TCATTGTGGCTAACAACTCC+AGG - tig0031748:17876-17895 None:intergenic 45.0%
CTCAAGAGTGAAGTTCTTCC+AGG - tig0031748:17940-17959 None:intergenic 45.0%
CTGGAAGAACTTCACTCTTG+AGG + tig0031748:17938-17957 Msa1447400:CDS 45.0%
CACTCTTGAGGAACAAGCTA+AGG + tig0031748:17950-17969 Msa1447400:CDS 45.0%
! AGTTTGAGCGAGAGCTTTTG+CGG + tig0031748:18306-18325 Msa1447400:three_prime_UTR 45.0%
GGAGTGAACAATGATTCAGC+AGG + tig0031748:18338-18357 Msa1447400:three_prime_UTR 45.0%
TGATTCAGCAGGATCAATGC+AGG + tig0031748:18349-18368 Msa1447400:three_prime_UTR 45.0%
TCAATCGATATGGAGCACGA+TGG - tig0031748:16361-16380 None:intergenic 45.0%
GCTGGGAAACCCATGATATT+TGG - tig0031748:16526-16545 None:intergenic 45.0%
GGCCGTATATGAGAAACTTG+AGG - tig0031748:16573-16592 None:intergenic 45.0%
CGTATTCGAATTGGATCCCT+TGG - tig0031748:16639-16658 None:intergenic 45.0%
GATCCAATTCGAATACGGGT+CGG + tig0031748:16642-16661 Msa1447400:CDS 45.0%
ATCCAATTCGAATACGGGTC+GGG + tig0031748:16643-16662 Msa1447400:CDS 45.0%
GCAGCATTAAACACGTGTGT+TGG - tig0031748:16718-16737 None:intergenic 45.0%
ACACACGTGTTTAATGCTGC+TGG + tig0031748:16718-16737 Msa1447400:CDS 45.0%
ACTATCCGTGCTAATGTGGT+TGG + tig0031748:16790-16809 Msa1447400:CDS 45.0%
CTATCCGTGCTAATGTGGTT+GGG + tig0031748:16791-16810 Msa1447400:CDS 45.0%
!!! TGGATCCGGGATTGGTTTTA+AGG + tig0031748:16909-16928 Msa1447400:CDS 45.0%
TTACTCCAAGACCAAGGCTA+TGG + tig0031748:16963-16982 Msa1447400:CDS 45.0%
! ACATACCATAGCCTTGGTCT+TGG - tig0031748:16971-16990 None:intergenic 45.0%
! TGCAGTAGTTGGAGTATCCT+TGG + tig0031748:17305-17324 Msa1447400:intron 45.0%
! GTTGGAGTATCCTTGGTTGT+CGG + tig0031748:17312-17331 Msa1447400:intron 45.0%
TGATCTTGATCCGACAACCA+AGG - tig0031748:17325-17344 None:intergenic 45.0%
ATGTATGCACCTTGAGAGTG+AGG + tig0031748:17682-17701 Msa1447400:CDS 45.0%
AATAGGCATCCTCACTCTCA+AGG - tig0031748:17694-17713 None:intergenic 45.0%
TGTGATGAAATTGCGAGGGT+TGG - tig0031748:17730-17749 None:intergenic 45.0%
ATGGCAAAGAGAAACCTCAC+TGG + tig0031748:17831-17850 Msa1447400:CDS 45.0%
AGAGAAACCTCACTGGAATC+TGG + tig0031748:17838-17857 Msa1447400:CDS 45.0%
TGAAGTTCCAGATTCCAGTG+AGG - tig0031748:17848-17867 None:intergenic 45.0%
TCATTGTGGCTAACAACTCC+AGG - tig0031748:17876-17895 None:intergenic 45.0%
CTCAAGAGTGAAGTTCTTCC+AGG - tig0031748:17940-17959 None:intergenic 45.0%
CTGGAAGAACTTCACTCTTG+AGG + tig0031748:17938-17957 Msa1447400:CDS 45.0%
CACTCTTGAGGAACAAGCTA+AGG + tig0031748:17950-17969 Msa1447400:CDS 45.0%
! AGTTTGAGCGAGAGCTTTTG+CGG + tig0031748:18306-18325 Msa1447400:three_prime_UTR 45.0%
GGAGTGAACAATGATTCAGC+AGG + tig0031748:18338-18357 Msa1447400:three_prime_UTR 45.0%
TGATTCAGCAGGATCAATGC+AGG + tig0031748:18349-18368 Msa1447400:three_prime_UTR 45.0%
AATACGCATCGAGAGAGCAG+AGG + tig0031748:16441-16460 Msa1447400:five_prime_UTR 50.0%
TGAGAGGAGAGAGAGAGACA+CGG - tig0031748:16493-16512 None:intergenic 50.0%
! GAGAAACTTGAGGGGAGTGT+CGG - tig0031748:16563-16582 None:intergenic 50.0%
TTTCTCATATACGGCCGCAC+CGG + tig0031748:16577-16596 Msa1447400:CDS 50.0%
CGGTACAGAGTTTGCCTAAG+AGG - tig0031748:16615-16634 None:intergenic 50.0%
TTCGAATTGGATCCCTTGGG+CGG - tig0031748:16635-16654 None:intergenic 50.0%
GAGTCCCAACCACATTAGCA+CGG - tig0031748:16798-16817 None:intergenic 50.0%
CATCTTCTTGGATCCGGGAT+TGG + tig0031748:16901-16920 Msa1447400:CDS 50.0%
AATACGCATCGAGAGAGCAG+AGG + tig0031748:16441-16460 Msa1447400:five_prime_UTR 50.0%
TGAGAGGAGAGAGAGAGACA+CGG - tig0031748:16493-16512 None:intergenic 50.0%
! GAGAAACTTGAGGGGAGTGT+CGG - tig0031748:16563-16582 None:intergenic 50.0%
TTTCTCATATACGGCCGCAC+CGG + tig0031748:16577-16596 Msa1447400:CDS 50.0%
CGGTACAGAGTTTGCCTAAG+AGG - tig0031748:16615-16634 None:intergenic 50.0%
TTCGAATTGGATCCCTTGGG+CGG - tig0031748:16635-16654 None:intergenic 50.0%
GAGTCCCAACCACATTAGCA+CGG - tig0031748:16798-16817 None:intergenic 50.0%
CATCTTCTTGGATCCGGGAT+TGG + tig0031748:16901-16920 Msa1447400:CDS 50.0%
!! AAACTTGAGGGGAGTGTCGG+AGG - tig0031748:16560-16579 None:intergenic 55.0%
TAAGAGGCCTCCTATCCAGC+CGG - tig0031748:16599-16618 None:intergenic 55.0%
GCAAACTCTGTACCGCCCAA+GGG + tig0031748:16620-16639 Msa1447400:CDS 55.0%
GACCCGACCCGTATTCGAAT+TGG - tig0031748:16648-16667 None:intergenic 55.0%
GTCTCACACCAGTCCACGTT+GGG - tig0031748:16757-16776 None:intergenic 55.0%
AGTCTCACACCAGTCCACGT+TGG - tig0031748:16758-16777 None:intergenic 55.0%
CACAGGTAGAGGATCTGCTG+AGG + tig0031748:17649-17668 Msa1447400:intron 55.0%
!! AAACTTGAGGGGAGTGTCGG+AGG - tig0031748:16560-16579 None:intergenic 55.0%
TAAGAGGCCTCCTATCCAGC+CGG - tig0031748:16599-16618 None:intergenic 55.0%
GCAAACTCTGTACCGCCCAA+GGG + tig0031748:16620-16639 Msa1447400:CDS 55.0%
GACCCGACCCGTATTCGAAT+TGG - tig0031748:16648-16667 None:intergenic 55.0%
GTCTCACACCAGTCCACGTT+GGG - tig0031748:16757-16776 None:intergenic 55.0%
AGTCTCACACCAGTCCACGT+TGG - tig0031748:16758-16777 None:intergenic 55.0%
CACAGGTAGAGGATCTGCTG+AGG + tig0031748:17649-17668 Msa1447400:intron 55.0%
GGCTGGATAGGAGGCCTCTT+AGG + tig0031748:16598-16617 Msa1447400:CDS 60.0%
GGCAAACTCTGTACCGCCCA+AGG + tig0031748:16619-16638 Msa1447400:CDS 60.0%
TGTTACGGGTCGACCCAACG+TGG + tig0031748:16741-16760 Msa1447400:CDS 60.0%
GGCTGGATAGGAGGCCTCTT+AGG + tig0031748:16598-16617 Msa1447400:CDS 60.0%
GGCAAACTCTGTACCGCCCA+AGG + tig0031748:16619-16638 Msa1447400:CDS 60.0%
TGTTACGGGTCGACCCAACG+TGG + tig0031748:16741-16760 Msa1447400:CDS 60.0%
CGGAGGAGGAGGATGGTGCT+GGG - tig0031748:16543-16562 None:intergenic 65.0%
TCGGAGGAGGAGGATGGTGC+TGG - tig0031748:16544-16563 None:intergenic 65.0%
!! GGAGTGTCGGAGGAGGAGGA+TGG - tig0031748:16550-16569 None:intergenic 65.0%
!! CTTGAGGGGAGTGTCGGAGG+AGG - tig0031748:16557-16576 None:intergenic 65.0%
! TCATATACGGCCGCACCGGC+TGG + tig0031748:16581-16600 Msa1447400:CDS 65.0%
GCTGACGTGTGCCGTGAACG+TGG + tig0031748:16826-16845 Msa1447400:CDS 65.0%
CGGAGGAGGAGGATGGTGCT+GGG - tig0031748:16543-16562 None:intergenic 65.0%
TCGGAGGAGGAGGATGGTGC+TGG - tig0031748:16544-16563 None:intergenic 65.0%
!! GGAGTGTCGGAGGAGGAGGA+TGG - tig0031748:16550-16569 None:intergenic 65.0%
!! CTTGAGGGGAGTGTCGGAGG+AGG - tig0031748:16557-16576 None:intergenic 65.0%
! TCATATACGGCCGCACCGGC+TGG + tig0031748:16581-16600 Msa1447400:CDS 65.0%
GCTGACGTGTGCCGTGAACG+TGG + tig0031748:16826-16845 Msa1447400:CDS 65.0%
!! GAGGGGAGTGTCGGAGGAGG+AGG - tig0031748:16554-16573 None:intergenic 70.0%
!! TACGGCCGCACCGGCTGGAT+AGG + tig0031748:16586-16605 Msa1447400:CDS 70.0%
GGCCTCCTATCCAGCCGGTG+CGG - tig0031748:16594-16613 None:intergenic 70.0%
CGGGTCGACCCAACGTGGAC+TGG + tig0031748:16746-16765 Msa1447400:CDS 70.0%
!! GAGGGGAGTGTCGGAGGAGG+AGG - tig0031748:16554-16573 None:intergenic 70.0%
!! TACGGCCGCACCGGCTGGAT+AGG + tig0031748:16586-16605 Msa1447400:CDS 70.0%
GGCCTCCTATCCAGCCGGTG+CGG - tig0031748:16594-16613 None:intergenic 70.0%
CGGGTCGACCCAACGTGGAC+TGG + tig0031748:16746-16765 Msa1447400:CDS 70.0%
!! GGCCGCACCGGCTGGATAGG+AGG + tig0031748:16589-16608 Msa1447400:CDS 75.0%
!! GGCCGCACCGGCTGGATAGG+AGG + tig0031748:16589-16608 Msa1447400:CDS 75.0%
Chromosome Type Strat End Strand Name
tig0031748 gene 16351 18395 16351 ID=Msa1447400;Name=Msa1447400
tig0031748 mRNA 16351 18395 16351 ID=Msa1447400-mRNA-1;Parent=Msa1447400;Name=Msa1447400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=181|1|1|1|1|1|2|292|300
tig0031748 exon 16351 16984 16351 ID=Msa1447400-mRNA-1:exon:15887;Parent=Msa1447400-mRNA-1
tig0031748 exon 17654 18395 17654 ID=Msa1447400-mRNA-1:exon:15888;Parent=Msa1447400-mRNA-1
tig0031748 five_prime_UTR 16351 16531 16351 ID=Msa1447400-mRNA-1:five_prime_utr;Parent=Msa1447400-mRNA-1
tig0031748 CDS 16532 16984 16532 ID=Msa1447400-mRNA-1:cds;Parent=Msa1447400-mRNA-1
tig0031748 CDS 17654 18103 17654 ID=Msa1447400-mRNA-1:cds;Parent=Msa1447400-mRNA-1
tig0031748 three_prime_UTR 18104 18395 18104 ID=Msa1447400-mRNA-1:three_prime_utr;Parent=Msa1447400-mRNA-1
Gene Sequence

>Msa1447400

ATGGGTTTCCCAGCACCATCCTCCTCCTCCGACACTCCCCTCAAGTTTCTCATATACGGCCGCACCGGCTGGATAGGAGGCCTCTTAGGCAAACTCTGTACCGCCCAAGGGATCCAATTCGAATACGGGTCGGGTCGTCTTGAGAATCGATCTTCATTGGAATCTGACATAGCTGAGATTAAACCAACACACGTGTTTAATGCTGCTGGTGTTACGGGTCGACCCAACGTGGACTGGTGTGAGACTCATAAAGTTGAAACTATCCGTGCTAATGTGGTTGGGACTCTCACTCTTGCTGACGTGTGCCGTGAACGTGGACTTATTGTTATTAATTTTGCTACTGGATGTATTTTTGAGTATGATGATCTTCATCTTCTTGGATCCGGGATTGGTTTTAAGGAAGAAGATACTCCTAATTTCATTGGTTCTTTTTACTCCAAGACCAAGGCTATGGTAGAGGATCTGCTGAGGAACTACGATAATGTATGCACCTTGAGAGTGAGGATGCCTATTTCATCTGATTTATCCAACCCTCGCAATTTCATCACAAAGATCACCCGATATGAGAAGGTTGTAGATATTCCAAACTCGATGACCATTTTAGATGAACTTCTTCCCATCTCAATTGAGATGGCAAAGAGAAACCTCACTGGAATCTGGAACTTCACAAATCCTGGAGTTGTTAGCCACAATGAAATCTTACAAATGTACAAAGACTATATCGATTCAAGCTTCACCTGGAAGAACTTCACTCTTGAGGAACAAGCTAAGGTGATAGTTGCCCCAAGAAGTAACAATGAGCTTGATGCGGGAAAATTGAAGAAGGAATTTCCAGAACTATTGTCTATCAAGGAATCACTCATTGAGAATGTCTTCAAGCCAAATCAGAAGGTTAAAGCTTGA
Protein sequence

>Msa1447400

MGFPAPSSSSDTPLKFLIYGRTGWIGGLLGKLCTAQGIQFEYGSGRLENRSSLESDIAEIKPTHVFNAAGVTGRPNVDWCETHKVETIRANVVGTLTLADVCRERGLIVINFATGCIFEYDDLHLLGSGIGFKEEDTPNFIGSFYSKTKAMVEDLLRNYDNVCTLRVRMPISSDLSNPRNFITKITRYEKVVDIPNSMTILDELLPISIEMAKRNLTGIWNFTNPGVVSHNEILQMYKDYIDSSFTWKNFTLEEQAKVIVAPRSNNELDAGKLKKEFPELLSIKESLIENVFKPNQKVKA*