AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1453270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453270 MtrunA17_Chr2g0329041 88.235 255 9 1 1 234 116 370 3.89e-159 449
Msa1453270 MtrunA17_Chr4g0032951 48.077 260 82 9 3 234 159 393 1.30e-66 214
Msa1453270 MtrunA17_Chr1g0174271 39.442 251 100 9 22 234 100 336 5.76e-36 132
Msa1453270 MtrunA17_Chr7g0258181 33.040 227 105 7 42 244 87 290 4.78e-15 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453270 AT2G42280.1 33.745 243 96 9 22 234 110 317 1.15e-21 94.0
Msa1453270 AT2G42280.3 32.463 268 109 11 10 234 79 317 1.34e-21 93.6
Msa1453270 AT1G51140.1 32.394 213 95 8 48 233 151 341 2.30e-15 75.9
Msa1453270 AT2G42280.2 30.769 195 95 8 30 213 110 275 5.40e-12 65.5

Find 78 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCTTTCTTCAACAATTA+TGG 0.131409 tig0034564:+2395 Msa1453270:three_prime_UTR
TAGATCCAATTTAAGCATTT+AGG 0.157031 tig0034564:+2509 Msa1453270:three_prime_UTR
TGTACAGATTTGTAATAATT+TGG 0.171797 tig0034564:+2252 Msa1453270:three_prime_UTR
GCCATTTCAGATGAAGTATT+TGG 0.195078 tig0034564:-1345 Msa1453270:intergenic
TTCCTTGAAGTAGAAAATTT+TGG 0.197445 tig0034564:-937 Msa1453270:intergenic
CGTACATATTTGTTATTATA+CGG 0.199213 tig0034564:-2565 Msa1453270:intergenic
ATAGATTTCATCACCAAATA+TGG 0.204816 tig0034564:-2534 Msa1453270:intergenic
TGGGCCCTAAATGCTTAAAT+TGG 0.246809 tig0034564:-2514 Msa1453270:intergenic
AGATCCAATTTAAGCATTTA+GGG 0.267087 tig0034564:+2510 Msa1453270:three_prime_UTR
AAGCATTTAGGGCCCATATT+TGG 0.290567 tig0034564:+2521 Msa1453270:three_prime_UTR
CTTCTTTCTTCAACAATTAT+GGG 0.299148 tig0034564:+2396 Msa1453270:three_prime_UTR
GGGGTGAATGAATTTATCTT+TGG 0.299991 tig0034564:-2330 Msa1453270:intergenic
CGTTTACGTTTCACATTGAA+TGG 0.311932 tig0034564:+2586 Msa1453270:three_prime_UTR
CATGGTACCTTATCCATGTT+TGG 0.320678 tig0034564:-1868 Msa1453270:intergenic
TAAATTGGCTTCAGGTTAGA+AGG 0.325551 tig0034564:+1803 Msa1453270:intron
ATGGTTGATTAGAATGAAAC+AGG 0.344190 tig0034564:+1279 Msa1453270:intron
AATGAAGAAAATGAAGCTTT+TGG 0.348594 tig0034564:+1039 Msa1453270:CDS
ATGAGAGGAATGGGAACTTT+AGG 0.362085 tig0034564:+889 Msa1453270:CDS
GAAGATGGAGATAAATTAGT+AGG 0.368545 tig0034564:+2367 Msa1453270:three_prime_UTR
GCCAAATACTTCATCTGAAA+TGG 0.370699 tig0034564:+1344 Msa1453270:CDS
AAGTCATGGTAACGAATATA+TGG 0.373522 tig0034564:+1065 Msa1453270:CDS
ATATAATGAATTTGTATTTG+AGG 0.389307 tig0034564:+2670 Msa1453270:three_prime_UTR
AGGACATTCTAATTCTCCTT+TGG 0.389735 tig0034564:+1305 Msa1453270:CDS
TCTTCAATGTTGGTGGTGTT+TGG 0.389909 tig0034564:+2725 Msa1453270:three_prime_UTR
AATCAATCAAACTATTCATC+AGG 0.391137 tig0034564:+967 Msa1453270:CDS
TAACGAATATATGGATTATC+AGG 0.397601 tig0034564:+1074 Msa1453270:CDS
TATTTGGTGATGAAATCTAT+TGG 0.406590 tig0034564:+2537 Msa1453270:three_prime_UTR
AACAATTATGGGAACTAGCT+AGG 0.422253 tig0034564:+2407 Msa1453270:three_prime_UTR
TGGATTATCAGGTTGATACT+TGG 0.436048 tig0034564:+1085 Msa1453270:CDS
TTCATCTGAAATGGCAGCTA+TGG 0.443352 tig0034564:+1353 Msa1453270:CDS
CTAAAATTAGTGAGCGTATG+AGG 0.443405 tig0034564:+1827 Msa1453270:CDS
AATTAAGTTGAGTGTAATTG+AGG 0.448480 tig0034564:+2448 Msa1453270:three_prime_UTR
TAACAAATAAAAGCTAATCT+AGG 0.452171 tig0034564:-2295 Msa1453270:intergenic
AGCAGCAACAACAACAATAA+CGG 0.452797 tig0034564:+2202 Msa1453270:CDS
CATCTTCATTATGTACATCA+AGG 0.461161 tig0034564:-2351 Msa1453270:intergenic
ATGGTACCTTATCCATGTTT+GGG 0.462347 tig0034564:-1867 Msa1453270:intergenic
TATGTTAGTATGAGAGGAAT+GGG 0.466243 tig0034564:+880 Msa1453270:CDS
TATGAGGAAGCTACAAGATC+TGG 0.475457 tig0034564:+1843 Msa1453270:CDS
TACGTTTCACATTGAATGGT+TGG 0.486627 tig0034564:+2590 Msa1453270:three_prime_UTR
AATGAAAAGAATAATATAAA+CGG 0.487875 tig0034564:-2697 Msa1453270:intergenic
TAGATTTCATCACCAAATAT+GGG 0.495278 tig0034564:-2533 Msa1453270:intergenic
TCTGGTCCCAAACATGGATA+AGG 0.497918 tig0034564:+1861 Msa1453270:CDS
GTTGTTGTTGCTGCTTGTGT+GGG 0.500762 tig0034564:-2194 Msa1453270:intergenic
ATTAAGTTGAGTGTAATTGA+GGG 0.507443 tig0034564:+2449 Msa1453270:three_prime_UTR
CTTGATGTACATAATGAAGA+TGG 0.510561 tig0034564:+2352 Msa1453270:three_prime_UTR
TGTTGTTGTTGCTGCTTGTG+TGG 0.515976 tig0034564:-2195 Msa1453270:intergenic
CACAGCAGATATGTTGGACT+TGG 0.518004 tig0034564:+1996 Msa1453270:CDS
TGGTGTTTGGTAAAGCCAAC+TGG 0.518331 tig0034564:+2738 Msa1453270:three_prime_UTR
TCACATTGAATGGTTGGCGT+TGG 0.523297 tig0034564:+2596 Msa1453270:three_prime_UTR
GCAGGTTATGTTAGTATGAG+AGG 0.524043 tig0034564:+874 Msa1453270:intron
ATCTTCATTATGTACATCAA+GGG 0.529039 tig0034564:-2350 Msa1453270:intergenic
GGATTATCAGGTTGATACTT+GGG 0.529420 tig0034564:+1086 Msa1453270:CDS
AACTAGCTAGGGAAGAAAAG+TGG 0.533515 tig0034564:+2419 Msa1453270:three_prime_UTR
AACAAACACAGCAGATATGT+TGG 0.533839 tig0034564:+1990 Msa1453270:CDS
GCGTTGGATTATAACATGGT+GGG 0.535962 tig0034564:+2612 Msa1453270:three_prime_UTR
AAGATGGAGATAAATTAGTA+GGG 0.538731 tig0034564:+2368 Msa1453270:three_prime_UTR
ACAATTATGGGAACTAGCTA+GGG 0.545940 tig0034564:+2408 Msa1453270:three_prime_UTR
AGATATGTTGGACTTGGCTG+TGG 0.548724 tig0034564:+2002 Msa1453270:CDS
AATTATTACAAATCTGTACA+TGG 0.552431 tig0034564:-2249 Msa1453270:intergenic
AGACCTTCAAAAGCAAGCTC+AGG 0.554514 tig0034564:+2035 Msa1453270:CDS
TTATGTTAGTATGAGAGGAA+TGG 0.558938 tig0034564:+879 Msa1453270:CDS
ATGTCAACAATAGCTGAAGT+TGG 0.572331 tig0034564:+994 Msa1453270:CDS
GGCGTTGGATTATAACATGG+TGG 0.573748 tig0034564:+2611 Msa1453270:three_prime_UTR
ACAAGATCTGGTCCCAAACA+TGG 0.576424 tig0034564:+1855 Msa1453270:CDS
ATCAATCAAACTATTCATCA+GGG 0.580340 tig0034564:+968 Msa1453270:CDS
ATAGCTGAAGTTGGTGACAA+AGG 0.592602 tig0034564:+1003 Msa1453270:CDS
AATCTAGGTAGATTCTAACT+TGG 0.597414 tig0034564:-2280 Msa1453270:intergenic
AATTGGATCTACTTACCACA+TGG 0.601595 tig0034564:-2497 Msa1453270:intergenic
GATTAGAATGAAACAGGAGG+AGG 0.610884 tig0034564:+1285 Msa1453270:intron
ACTAGCTAGGGAAGAAAAGT+GGG 0.615706 tig0034564:+2420 Msa1453270:three_prime_UTR
TGCAAATGAGAACCACCAGT+TGG 0.617548 tig0034564:-2753 Msa1453270:intergenic
ATGATGTCTGAAAATGTTGG+TGG 0.620048 tig0034564:+1120 Msa1453270:CDS
ATACTCAATTGATGAGCCAA+AGG 0.626112 tig0034564:-1321 Msa1453270:intergenic
TCTTCATTATGTACATCAAG+GGG 0.648537 tig0034564:-2349 Msa1453270:intergenic
GAAATGATGTCTGAAAATGT+TGG 0.668717 tig0034564:+1117 Msa1453270:CDS
GTTGATTAGAATGAAACAGG+AGG 0.674543 tig0034564:+1282 Msa1453270:intron
TGTTTGGTAAAGCCAACTGG+TGG 0.692158 tig0034564:+2741 Msa1453270:three_prime_UTR
GTTGGCGTTGGATTATAACA+TGG 0.729880 tig0034564:+2608 Msa1453270:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTAGTTATTACTTATT+AGG + tig0034564:776-795 Msa1453270:intron 10.0%
!! TTAAAGAAATAAAATAGTTT+GGG - tig0034564:815-834 Msa1453270:intergenic 10.0%
!! AATGAAAAGAATAATATAAA+CGG - tig0034564:2700-2719 Msa1453270:intergenic 10.0%
!!! TATTTTTTTCTCTTTAAAGT+AGG - tig0034564:573-592 Msa1453270:intergenic 15.0%
!!! TTCAAATTTAACATTTTTCT+AGG - tig0034564:603-622 Msa1453270:intergenic 15.0%
!! TATATATTAAAGATCTCTTT+AGG + tig0034564:656-675 Msa1453270:intron 15.0%
!! ATATATTAAAGATCTCTTTA+GGG + tig0034564:657-676 Msa1453270:intron 15.0%
!!! TTTTAATATTTGCTATTATG+TGG + tig0034564:691-710 Msa1453270:intron 15.0%
!! TAAAGAAATAAAATAGTTTG+GGG - tig0034564:814-833 Msa1453270:intergenic 15.0%
!! GTTAAAGAAATAAAATAGTT+TGG - tig0034564:816-835 Msa1453270:intergenic 15.0%
!! AACAAAAGAAAAGTAATATA+GGG - tig0034564:1643-1662 Msa1453270:intergenic 15.0%
!! AAACAAAAGAAAAGTAATAT+AGG - tig0034564:1644-1663 Msa1453270:intergenic 15.0%
!!! ATATTACTTTTCTTTTGTTT+AGG + tig0034564:1644-1663 Msa1453270:intron 15.0%
!! CTAAAACTAAATATGTAAAT+TGG - tig0034564:1680-1699 Msa1453270:intergenic 15.0%
!! ATATAATGAATTTGTATTTG+AGG + tig0034564:2670-2689 Msa1453270:three_prime_UTR 15.0%
!! TCTCAAATTAACATTGAAAA+TGG + tig0034564:182-201 Msa1453270:CDS 20.0%
!! TGAAGAAAATGAATTGTAAA+AGG + tig0034564:443-462 Msa1453270:intron 20.0%
!!! AATTTGAAAATTTTCTTGTC+AGG + tig0034564:615-634 Msa1453270:intron 20.0%
!!! ATTTGAAAATTTTCTTGTCA+GGG + tig0034564:616-635 Msa1453270:intron 20.0%
!!! TACTATTAAGACATGATTTT+TGG + tig0034564:1705-1724 Msa1453270:intron 20.0%
!!! ACTATTAAGACATGATTTTT+GGG + tig0034564:1706-1725 Msa1453270:intron 20.0%
!! CATTCACATAAATTAATGTA+TGG - tig0034564:1930-1949 Msa1453270:intergenic 20.0%
!! AATTATTACAAATCTGTACA+TGG - tig0034564:2252-2271 Msa1453270:intergenic 20.0%
!! TGTACAGATTTGTAATAATT+TGG + tig0034564:2252-2271 Msa1453270:three_prime_UTR 20.0%
!! TAACAAATAAAAGCTAATCT+AGG - tig0034564:2298-2317 Msa1453270:intergenic 20.0%
!!! CGTACATATTTGTTATTATA+CGG - tig0034564:2568-2587 Msa1453270:intergenic 20.0%
!!! TTTAGATGGAAGTTTTTCAA+TGG + tig0034564:73-92 Msa1453270:CDS 25.0%
!!! TTAGATGGAAGTTTTTCAAT+GGG + tig0034564:74-93 Msa1453270:CDS 25.0%
! CTCGTTTCTATTAAAATAGA+GGG - tig0034564:257-276 Msa1453270:intergenic 25.0%
! TCTCGTTTCTATTAAAATAG+AGG - tig0034564:258-277 Msa1453270:intergenic 25.0%
! TGTGACTAAAATTTCAGATT+TGG - tig0034564:295-314 Msa1453270:intergenic 25.0%
! TCTAATAAAATCCACCATTT+CGG - tig0034564:395-414 Msa1453270:intergenic 25.0%
!! ATGAATTGTAAAAGGTTTTC+TGG + tig0034564:451-470 Msa1453270:intron 25.0%
!! TTTGAAAATTTTCTTGTCAG+GGG + tig0034564:617-636 Msa1453270:intron 25.0%
! TGAAAAAAAAAAACCCTAGT+GGG + tig0034564:753-772 Msa1453270:intron 25.0%
! AATAACTAATATTCCCACTA+GGG - tig0034564:769-788 Msa1453270:intergenic 25.0%
! TAATAACTAATATTCCCACT+AGG - tig0034564:770-789 Msa1453270:intergenic 25.0%
! TGATGTTATGCAAGTTTAAT+TGG + tig0034564:852-871 Msa1453270:intron 25.0%
!! AACCAAAATTTTCTACTTCA+AGG + tig0034564:935-954 Msa1453270:CDS 25.0%
!! TTCCTTGAAGTAGAAAATTT+TGG - tig0034564:940-959 Msa1453270:intergenic 25.0%
! AATCAATCAAACTATTCATC+AGG + tig0034564:967-986 Msa1453270:CDS 25.0%
! ATCAATCAAACTATTCATCA+GGG + tig0034564:968-987 Msa1453270:CDS 25.0%
!! AATGAAGAAAATGAAGCTTT+TGG + tig0034564:1039-1058 Msa1453270:CDS 25.0%
!! TAACGAATATATGGATTATC+AGG + tig0034564:1074-1093 Msa1453270:CDS 25.0%
!! GATTCAAAACAACAATTTTC+TGG + tig0034564:1165-1184 Msa1453270:CDS 25.0%
!!! ATTTTCTGGTTTAAATGTTC+AGG + tig0034564:1179-1198 Msa1453270:CDS 25.0%
!! CATTTGACATGATTTTGTTA+TGG + tig0034564:1260-1279 Msa1453270:intron 25.0%
!!! TTTTTACCTTAGTTCTTATG+AGG + tig0034564:1490-1509 Msa1453270:intron 25.0%
! TAGAATTATCTATAACCAAG+TGG - tig0034564:1606-1625 Msa1453270:intergenic 25.0%
!! CTATTAAGACATGATTTTTG+GGG + tig0034564:1707-1726 Msa1453270:intron 25.0%
! CTCTTAACACATCATTAAAT+TGG + tig0034564:1788-1807 Msa1453270:intron 25.0%
! GTGTTGGAAGAAAATTAATT+AGG - tig0034564:2134-2153 Msa1453270:intergenic 25.0%
! ATCTTCATTATGTACATCAA+GGG - tig0034564:2353-2372 Msa1453270:intergenic 25.0%
! AAGATGGAGATAAATTAGTA+GGG + tig0034564:2368-2387 Msa1453270:three_prime_UTR 25.0%
! ACTTCTTTCTTCAACAATTA+TGG + tig0034564:2395-2414 Msa1453270:three_prime_UTR 25.0%
! CTTCTTTCTTCAACAATTAT+GGG + tig0034564:2396-2415 Msa1453270:three_prime_UTR 25.0%
!!! AATTAAGTTGAGTGTAATTG+AGG + tig0034564:2448-2467 Msa1453270:three_prime_UTR 25.0%
!!! ATTAAGTTGAGTGTAATTGA+GGG + tig0034564:2449-2468 Msa1453270:three_prime_UTR 25.0%
! TAGATCCAATTTAAGCATTT+AGG + tig0034564:2509-2528 Msa1453270:three_prime_UTR 25.0%
! AGATCCAATTTAAGCATTTA+GGG + tig0034564:2510-2529 Msa1453270:three_prime_UTR 25.0%
! TAGATTTCATCACCAAATAT+GGG - tig0034564:2536-2555 Msa1453270:intergenic 25.0%
! ATAGATTTCATCACCAAATA+TGG - tig0034564:2537-2556 Msa1453270:intergenic 25.0%
!!! TATTTGGTGATGAAATCTAT+TGG + tig0034564:2537-2556 Msa1453270:three_prime_UTR 25.0%
! TATAACCAACAAAAATGTGT+CGG - tig0034564:2648-2667 Msa1453270:intergenic 25.0%
!!! TCATTTTGTTTCTTCAATGT+TGG + tig0034564:2715-2734 Msa1453270:three_prime_UTR 25.0%
TGATAAAAACCATGAGAAGA+AGG - tig0034564:8-27 None:intergenic 30.0%
!! AATGTTTCTTCTGGTTTAGA+TGG + tig0034564:59-78 Msa1453270:CDS 30.0%
!! GTTTTTCAATGGGAGTTAAT+AGG + tig0034564:84-103 Msa1453270:CDS 30.0%
ATTTGAGAAAATAATCCAGC+TGG - tig0034564:170-189 Msa1453270:intergenic 30.0%
! TTTTCTTTGTTACGCACTTT+TGG + tig0034564:230-249 Msa1453270:intron 30.0%
!!! TACTGATTTTTGATCCGAAA+TGG + tig0034564:378-397 Msa1453270:intron 30.0%
!! TTTCTCTTTAAAGTAGGACA+AGG - tig0034564:567-586 Msa1453270:intergenic 30.0%
GTGAAAAAAAAAAACCCTAG+TGG + tig0034564:752-771 Msa1453270:intron 30.0%
TTATGTTAGTATGAGAGGAA+TGG + tig0034564:879-898 Msa1453270:CDS 30.0%
TATGTTAGTATGAGAGGAAT+GGG + tig0034564:880-899 Msa1453270:CDS 30.0%
! AAGTCATGGTAACGAATATA+TGG + tig0034564:1065-1084 Msa1453270:CDS 30.0%
GAAATGATGTCTGAAAATGT+TGG + tig0034564:1117-1136 Msa1453270:CDS 30.0%
TCGTAAGTGTAGTTTCTTAA+TGG + tig0034564:1232-1251 Msa1453270:intron 30.0%
ATGGTTGATTAGAATGAAAC+AGG + tig0034564:1279-1298 Msa1453270:intron 30.0%
ATCAATAATCCATACCCTTT+CGG - tig0034564:1460-1479 Msa1453270:intergenic 30.0%
CTCAAATGGTTAATAAGCTT+CGG - tig0034564:1579-1598 Msa1453270:intergenic 30.0%
GAACCTCAATGTACATATAA+TGG - tig0034564:1733-1752 Msa1453270:intergenic 30.0%
TTAAAACCAATCATGAAGCA+TGG - tig0034564:1889-1908 Msa1453270:intergenic 30.0%
GTGTTTGTTTGCTTCAAAAA+AGG - tig0034564:1979-1998 Msa1453270:intergenic 30.0%
AATCTAGGTAGATTCTAACT+TGG - tig0034564:2283-2302 Msa1453270:intergenic 30.0%
TCTTCATTATGTACATCAAG+GGG - tig0034564:2352-2371 Msa1453270:intergenic 30.0%
CATCTTCATTATGTACATCA+AGG - tig0034564:2354-2373 Msa1453270:intergenic 30.0%
CTTGATGTACATAATGAAGA+TGG + tig0034564:2352-2371 Msa1453270:three_prime_UTR 30.0%
GAAGATGGAGATAAATTAGT+AGG + tig0034564:2367-2386 Msa1453270:three_prime_UTR 30.0%
!! TTTTGTTTCTTCAATGTTGG+TGG + tig0034564:2718-2737 Msa1453270:three_prime_UTR 30.0%
!! GAAACATTCTGATTTGGCAA+AGG - tig0034564:47-66 None:intergenic 35.0%
CCAGAAGAAACATTCTGATT+TGG - tig0034564:53-72 None:intergenic 35.0%
CCAAATCAGAATGTTTCTTC+TGG + tig0034564:50-69 Msa1453270:CDS 35.0%
TTTCCACAATGATTCTTCAC+TGG - tig0034564:116-135 Msa1453270:intergenic 35.0%
! TGATTTTTGATCCGAAATGG+TGG + tig0034564:381-400 Msa1453270:intron 35.0%
TAGTACACGTTACTAGTATG+TGG + tig0034564:481-500 Msa1453270:intron 35.0%
TACTAGTATGTGGACTTGTA+AGG + tig0034564:491-510 Msa1453270:intron 35.0%
TATTGAAGTTGTACATGCCA+CGG - tig0034564:523-542 Msa1453270:intergenic 35.0%
AAAGTAGGACAAGGAAAACT+GGG - tig0034564:558-577 Msa1453270:intergenic 35.0%
TAAAGTAGGACAAGGAAAAC+TGG - tig0034564:559-578 Msa1453270:intergenic 35.0%
! TTTGTACTTGTTGCAACAAG+TGG + tig0034564:730-749 Msa1453270:intron 35.0%
GTTATGCAAGTTTAATTGGC+AGG + tig0034564:856-875 Msa1453270:intron 35.0%
ATGTCAACAATAGCTGAAGT+TGG + tig0034564:994-1013 Msa1453270:CDS 35.0%
!! TGGATTATCAGGTTGATACT+TGG + tig0034564:1085-1104 Msa1453270:CDS 35.0%
!! GGATTATCAGGTTGATACTT+GGG + tig0034564:1086-1105 Msa1453270:CDS 35.0%
ATGATGTCTGAAAATGTTGG+TGG + tig0034564:1120-1139 Msa1453270:CDS 35.0%
GGTAGAATACTATCTAGCAT+TGG + tig0034564:1200-1219 Msa1453270:intron 35.0%
GTTGATTAGAATGAAACAGG+AGG + tig0034564:1282-1301 Msa1453270:intron 35.0%
AGGACATTCTAATTCTCCTT+TGG + tig0034564:1305-1324 Msa1453270:CDS 35.0%
ATACTCAATTGATGAGCCAA+AGG - tig0034564:1324-1343 Msa1453270:intergenic 35.0%
GCCAAATACTTCATCTGAAA+TGG + tig0034564:1344-1363 Msa1453270:CDS 35.0%
! GCCATTTCAGATGAAGTATT+TGG - tig0034564:1348-1367 Msa1453270:intergenic 35.0%
TACTTGCCTCATAAGAACTA+AGG - tig0034564:1499-1518 Msa1453270:intergenic 35.0%
TGGTTAATAAGCTTCGGTTA+AGG - tig0034564:1573-1592 Msa1453270:intergenic 35.0%
! CACATCATTAAATTGGCTTC+AGG + tig0034564:1795-1814 Msa1453270:intron 35.0%
! TAAATTGGCTTCAGGTTAGA+AGG + tig0034564:1803-1822 Msa1453270:intron 35.0%
CTAAAATTAGTGAGCGTATG+AGG + tig0034564:1827-1846 Msa1453270:CDS 35.0%
ATGGTACCTTATCCATGTTT+GGG - tig0034564:1870-1889 Msa1453270:intergenic 35.0%
AACAAACACAGCAGATATGT+TGG + tig0034564:1990-2009 Msa1453270:CDS 35.0%
TGAAGCTTCTATACAAGTGT+TGG - tig0034564:2150-2169 Msa1453270:intergenic 35.0%
AGCAGCAACAACAACAATAA+CGG + tig0034564:2202-2221 Msa1453270:CDS 35.0%
GGGGTGAATGAATTTATCTT+TGG - tig0034564:2333-2352 Msa1453270:intergenic 35.0%
AACAATTATGGGAACTAGCT+AGG + tig0034564:2407-2426 Msa1453270:three_prime_UTR 35.0%
ACAATTATGGGAACTAGCTA+GGG + tig0034564:2408-2427 Msa1453270:three_prime_UTR 35.0%
GTATGTACAAAAAGTCCATG+TGG + tig0034564:2482-2501 Msa1453270:three_prime_UTR 35.0%
AATTGGATCTACTTACCACA+TGG - tig0034564:2500-2519 Msa1453270:intergenic 35.0%
CGTTTACGTTTCACATTGAA+TGG + tig0034564:2586-2605 Msa1453270:three_prime_UTR 35.0%
TACGTTTCACATTGAATGGT+TGG + tig0034564:2590-2609 Msa1453270:three_prime_UTR 35.0%
!! ACATTCTGATTTGGCAAAGG+TGG - tig0034564:44-63 None:intergenic 40.0%
GCAGGTTATGTTAGTATGAG+AGG + tig0034564:874-893 Msa1453270:intron 40.0%
ATGAGAGGAATGGGAACTTT+AGG + tig0034564:889-908 Msa1453270:CDS 40.0%
!! ATAGCTGAAGTTGGTGACAA+AGG + tig0034564:1003-1022 Msa1453270:CDS 40.0%
! GAAGCTTTTGGTGAAAGTCA+TGG + tig0034564:1051-1070 Msa1453270:CDS 40.0%
GATTAGAATGAAACAGGAGG+AGG + tig0034564:1285-1304 Msa1453270:intron 40.0%
! TTCATCTGAAATGGCAGCTA+TGG + tig0034564:1353-1372 Msa1453270:CDS 40.0%
AACCATTTGAGCAAACCACT+TGG + tig0034564:1588-1607 Msa1453270:intron 40.0%
!! AACCAAGTGGTTTGCTCAAA+TGG - tig0034564:1593-1612 Msa1453270:intergenic 40.0%
GGGCCATTATATGTACATTG+AGG + tig0034564:1727-1746 Msa1453270:intron 40.0%
TATGAGGAAGCTACAAGATC+TGG + tig0034564:1843-1862 Msa1453270:CDS 40.0%
CATGGTACCTTATCCATGTT+TGG - tig0034564:1871-1890 Msa1453270:intergenic 40.0%
! AAGGTACCATGCTTCATGAT+TGG + tig0034564:1880-1899 Msa1453270:intron 40.0%
!! ATACCTGAGCTTGCTTTTGA+AGG - tig0034564:2041-2060 Msa1453270:intergenic 40.0%
AACTAGCTAGGGAAGAAAAG+TGG + tig0034564:2419-2438 Msa1453270:three_prime_UTR 40.0%
ACTAGCTAGGGAAGAAAAGT+GGG + tig0034564:2420-2439 Msa1453270:three_prime_UTR 40.0%
TGGGCCCTAAATGCTTAAAT+TGG - tig0034564:2517-2536 Msa1453270:intergenic 40.0%
! AAGCATTTAGGGCCCATATT+TGG + tig0034564:2521-2540 Msa1453270:three_prime_UTR 40.0%
! GTTGGCGTTGGATTATAACA+TGG + tig0034564:2608-2627 Msa1453270:three_prime_UTR 40.0%
GCGTTGGATTATAACATGGT+GGG + tig0034564:2612-2631 Msa1453270:three_prime_UTR 40.0%
! ACAAAAATGTGTCGGTGTCT+CGG - tig0034564:2640-2659 Msa1453270:intergenic 40.0%
! AGACACCGACACATTTTTGT+TGG + tig0034564:2640-2659 Msa1453270:three_prime_UTR 40.0%
!! TCTTCAATGTTGGTGGTGTT+TGG + tig0034564:2725-2744 Msa1453270:three_prime_UTR 40.0%
CAGCCAGTGAAGAATCATTG+TGG + tig0034564:110-129 Msa1453270:CDS 45.0%
AGACATAGTAGTTCACCAGC+TGG + tig0034564:152-171 Msa1453270:CDS 45.0%
! CGCTTGGCACGAATTTTCAT+CGG - tig0034564:1408-1427 Msa1453270:intergenic 45.0%
ATGAAAATTCGTGCCAAGCG+AGG + tig0034564:1408-1427 Msa1453270:intron 45.0%
ACAAGATCTGGTCCCAAACA+TGG + tig0034564:1855-1874 Msa1453270:CDS 45.0%
TCTGGTCCCAAACATGGATA+AGG + tig0034564:1861-1880 Msa1453270:CDS 45.0%
CACAGCAGATATGTTGGACT+TGG + tig0034564:1996-2015 Msa1453270:CDS 45.0%
AGATATGTTGGACTTGGCTG+TGG + tig0034564:2002-2021 Msa1453270:CDS 45.0%
AGACCTTCAAAAGCAAGCTC+AGG + tig0034564:2035-2054 Msa1453270:CDS 45.0%
! GTTGTTGTTGCTGCTTGTGT+GGG - tig0034564:2197-2216 Msa1453270:intergenic 45.0%
! TGTTGTTGTTGCTGCTTGTG+TGG - tig0034564:2198-2217 Msa1453270:intergenic 45.0%
TCACATTGAATGGTTGGCGT+TGG + tig0034564:2596-2615 Msa1453270:three_prime_UTR 45.0%
! GGCGTTGGATTATAACATGG+TGG + tig0034564:2611-2630 Msa1453270:three_prime_UTR 45.0%
TGGTGTTTGGTAAAGCCAAC+TGG + tig0034564:2738-2757 Msa1453270:three_prime_UTR 45.0%
TGTTTGGTAAAGCCAACTGG+TGG + tig0034564:2741-2760 Msa1453270:three_prime_UTR 45.0%
TGCAAATGAGAACCACCAGT+TGG - tig0034564:2756-2775 Msa1453270:intergenic 45.0%
!! GACTTGTAAGGTACTGTCCG+TGG + tig0034564:503-522 Msa1453270:intron 50.0%
TTCGGCAATGCTTCTTGGGT+GGG - tig0034564:1442-1461 Msa1453270:intergenic 50.0%
TTTCGGCAATGCTTCTTGGG+TGG - tig0034564:1443-1462 Msa1453270:intergenic 50.0%
!! ACCCAAGAAGCATTGCCGAA+AGG + tig0034564:1442-1461 Msa1453270:intron 50.0%
CCCTTTCGGCAATGCTTCTT+GGG - tig0034564:1446-1465 Msa1453270:intergenic 50.0%
!! CCCAAGAAGCATTGCCGAAA+GGG + tig0034564:1443-1462 Msa1453270:intron 50.0%
ACCCTTTCGGCAATGCTTCT+TGG - tig0034564:1447-1466 Msa1453270:intergenic 50.0%
!! GAAGCATTGCCGAAAGGGTA+TGG + tig0034564:1448-1467 Msa1453270:intron 50.0%
GGCAATGCTTCTTGGGTGGG+TGG - tig0034564:1439-1458 Msa1453270:intergenic 60.0%
GTGGGTGGCACAACCTCGCT+TGG - tig0034564:1424-1443 Msa1453270:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0034564 gene 2 2848 2 ID=Msa1453270;Name=Msa1453270
tig0034564 mRNA 2 2848 2 ID=Msa1453270-mRNA-1;Parent=Msa1453270;Name=Msa1453270-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0.6|0.5|1|0.6|0.66|6|601|295
tig0034564 exon 2 203 2 ID=Msa1453270-mRNA-1:exon:19071;Parent=Msa1453270-mRNA-1
tig0034564 exon 878 1200 878 ID=Msa1453270-mRNA-1:exon:19072;Parent=Msa1453270-mRNA-1
tig0034564 exon 1291 1401 1291 ID=Msa1453270-mRNA-1:exon:19073;Parent=Msa1453270-mRNA-1
tig0034564 exon 1817 1882 1817 ID=Msa1453270-mRNA-1:exon:19074;Parent=Msa1453270-mRNA-1
tig0034564 exon 1957 2056 1957 ID=Msa1453270-mRNA-1:exon:19075;Parent=Msa1453270-mRNA-1
tig0034564 exon 2162 2848 2162 ID=Msa1453270-mRNA-1:exon:19076;Parent=Msa1453270-mRNA-1
tig0034564 CDS 2 203 2 ID=Msa1453270-mRNA-1:cds;Parent=Msa1453270-mRNA-1
tig0034564 CDS 878 1200 878 ID=Msa1453270-mRNA-1:cds;Parent=Msa1453270-mRNA-1
tig0034564 CDS 1291 1401 1291 ID=Msa1453270-mRNA-1:cds;Parent=Msa1453270-mRNA-1
tig0034564 CDS 1817 1882 1817 ID=Msa1453270-mRNA-1:cds;Parent=Msa1453270-mRNA-1
tig0034564 CDS 1957 2056 1957 ID=Msa1453270-mRNA-1:cds;Parent=Msa1453270-mRNA-1
tig0034564 CDS 2162 2247 2162 ID=Msa1453270-mRNA-1:cds;Parent=Msa1453270-mRNA-1
tig0034564 three_prime_UTR 2248 2848 2248 ID=Msa1453270-mRNA-1:three_prime_utr;Parent=Msa1453270-mRNA-1
Gene Sequence

>Msa1453270

GTTCCTTCTTCTCATGGTTTTTATCAAAGCTCTATGATGCCACCTTTGCCAAATCAGAATGTTTCTTCTGGTTTAGATGGAAGTTTTTCAATGGGAGTTAATAGGTTGCAGCCAGTGAAGAATCATTGTGGAAATAATTCAAATCTTATTAGACATAGTAGTTCACCAGCTGGATTATTTTCTCAAATTAACATTGAAAATGGTTATGTTAGTATGAGAGGAATGGGAACTTTAGGAGCTGTTAATAATTCTATGAAAGAACCAAAATTTTCTACTTCAAGGAATTTGAAGAATCAATCAAACTATTCATCAGGGTTAATGTCAACAATAGCTGAAGTTGGTGACAAAGGCAATAGAGAAAATAATGAAGAAAATGAAGCTTTTGGTGAAAGTCATGGTAACGAATATATGGATTATCAGGTTGATACTTGGGATGATTCTGAAATGATGTCTGAAAATGTTGGTGGTTTAAAAAGATTTAGAGATAATGATTCAAAACAACAATTTTCTGGTTTAAATGTTCAGAATGAAACAGGAGGAGGACATTCTAATTCTCCTTTGGCTCATCAATTGAGTATGCCAAATACTTCATCTGAAATGGCAGCTATGGAGAAGTTTCTGCATTTCTCAGATTCTGTTAGAAGGACTAAAATTAGTGAGCGTATGAGGAAGCTACAAGATCTGGTCCCAAACATGGATAAGATACAACGTTGTTTCTCTTCCTTTTTTGAAGCAAACAAACACAGCAGATATGTTGGACTTGGCTGTGGATTACATCAAAGACCTTCAAAAGCAAGCTCAGAAGCTTCAAGATTGTCAAGCAAAGTGTACGTGCCCACACAAGCAGCAACAACAACAATAACGGCAAAGAGTGAATTAATTAATTAG
Protein sequence

>Msa1453270

VPSSHGFYQSSMMPPLPNQNVSSGLDGSFSMGVNRLQPVKNHCGNNSNLIRHSSSPAGLFSQINIENGYVSMRGMGTLGAVNNSMKEPKFSTSRNLKNQSNYSSGLMSTIAEVGDKGNRENNEENEAFGESHGNEYMDYQVDTWDDSEMMSENVGGLKRFRDNDSKQQFSGLNVQNETGGGHSNSPLAHQLSMPNTSSEMAAMEKFLHFSDSVRRTKISERMRKLQDLVPNMDKIQRCFSSFFEANKHSRYVGLGCGLHQRPSKASSEASRLSSKVYVPTQAATTTITAKSELIN*