AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1454810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454810 MtrunA17_Chr7g0275851 96.996 233 6 1 1 232 75 307 4.30e-166 460
Msa1454810 MtrunA17_Chr8g0346691 40.000 215 90 9 5 205 4 193 7.82e-32 115
Msa1454810 MtrunA17_Chr3g0126981 33.333 186 80 8 43 221 9 157 1.61e-14 68.9
Msa1454810 MtrunA17_Chr1g0146921 33.668 199 86 10 42 226 44 210 2.36e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454810 AT4G27810.1 29.075 227 98 9 8 221 9 185 1.61e-12 64.7
Msa1454810 AT4G00950.1 29.097 299 123 16 1 221 1 288 3.73e-12 64.7

Find 113 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGTGCAAATGATATTTA+TGG 0.178655 tig0035450:+3358 Msa1454810:three_prime_UTR
ACATTCAACTTGTTGTTATT+TGG 0.225324 tig0035450:-3299 None:intergenic
TTGCAAAGCCTTGGCTTTCC+TGG 0.231150 tig0035450:-2389 None:intergenic
TATTTCCACACTTTCCTCTT+TGG 0.271077 tig0035450:+2072 Msa1454810:five_prime_UTR
ACAGTGGTTGGTTTGGTTCT+TGG 0.271528 tig0035450:+2636 Msa1454810:CDS
GTCACCGTCCCCGTTCTCTC+TGG 0.275715 tig0035450:-2317 None:intergenic
GTTACTTTCCGCTTTGTCTT+GGG 0.300396 tig0035450:-2016 None:intergenic
TGAGTTTGATATAACTGAAT+TGG 0.313740 tig0035450:-2101 None:intergenic
TCCATCTTCTTCTACTGATA+AGG 0.358897 tig0035450:+2706 Msa1454810:CDS
TGTTACTTTCCGCTTTGTCT+TGG 0.361454 tig0035450:-2017 None:intergenic
CAGTAAGATAATAATAATAA+AGG 0.368424 tig0035450:-2157 None:intergenic
ACAATTACTCCCCTCCCCTT+TGG 0.373588 tig0035450:+3199 Msa1454810:three_prime_UTR
CTATCGTAACGCCATTGAAT+TGG 0.374073 tig0035450:+3401 Msa1454810:three_prime_UTR
AAATGATATAAAAGATCATT+TGG 0.383714 tig0035450:-2132 None:intergenic
CTCCAAACACTTGTCACTAC+TGG 0.386183 tig0035450:-2435 None:intergenic
TTGTCTTCAATTCTAAAAGA+TGG 0.393325 tig0035450:-2539 None:intergenic
AGAAACTATGTTCATTATAG+TGG 0.402940 tig0035450:+3047 Msa1454810:three_prime_UTR
TCTTCTACTGATAAGGATAC+TGG 0.403705 tig0035450:+2713 Msa1454810:CDS
CCCCAGTAGTGACAAGTGTT+TGG 0.412430 tig0035450:+2433 Msa1454810:CDS
TCACCGTCCCCGTTCTCTCT+GGG 0.413186 tig0035450:-2316 None:intergenic
AAATCTATTCTCAAAGCTTA+AGG 0.419097 tig0035450:-2996 None:intergenic
AAAAGTTGAAGAAGAAAGAT+GGG 0.421291 tig0035450:+2968 Msa1454810:CDS
AAAAGAGACAGTGGTTGGTT+TGG 0.429365 tig0035450:+2629 Msa1454810:CDS
CATGAGGACCCTGCTGCAAA+AGG 0.430448 tig0035450:-2493 None:intergenic
GAAAGTGTGGAAATATGATT+AGG 0.431607 tig0035450:-2064 None:intergenic
ATGGAATCCCCAGAGAGAAC+GGG 0.434261 tig0035450:+2308 Msa1454810:CDS
CAGTACTGCAACAAATTAGC+TGG 0.434913 tig0035450:-3084 None:intergenic
GTTGTTGTCTGCAATGTCAA+TGG 0.436341 tig0035450:-2738 None:intergenic
AACAAGATGATGAAACGTTC+TGG 0.436883 tig0035450:+2758 Msa1454810:CDS
AAAGACAAGTACTAAGCAAA+TGG 0.443990 tig0035450:-3269 None:intergenic
GGTTCTTGTTCCCAACGAAA+TGG 0.450088 tig0035450:-2368 None:intergenic
AATGAAAATTCAAATGCAAA+TGG 0.451066 tig0035450:+2289 Msa1454810:CDS
ATGTAACCAGCAACAGATTC+CGG 0.462310 tig0035450:+2513 Msa1454810:CDS
TGGAGACCGGAATCTGTTGC+TGG 0.464392 tig0035450:-2519 None:intergenic
ATTACTAATTTCTACTAAGT+AGG 0.465210 tig0035450:-3173 None:intergenic
AGTACTGATAGAGGCTTACT+TGG 0.468153 tig0035450:+2584 Msa1454810:CDS
TCCAAACACTTGTCACTACT+GGG 0.470671 tig0035450:-2434 None:intergenic
AGGTTCAGTACCATTTCGTT+GGG 0.482945 tig0035450:+2358 Msa1454810:CDS
AATGGCGTTACGATAGAACT+TGG 0.483691 tig0035450:-3394 None:intergenic
CTGCTGCAAAAGGGTGGGTG+AGG 0.488417 tig0035450:-2483 None:intergenic
TCTTCGTTCAGTACTGATAG+AGG 0.489913 tig0035450:+2575 Msa1454810:CDS
GTGGTTGGTTTGGTTCTTGG+AGG 0.490179 tig0035450:+2639 Msa1454810:CDS
TCCATAAATATCATTTGCAC+TGG 0.490411 tig0035450:-3359 None:intergenic
CAGGTTCAGTACCATTTCGT+TGG 0.491351 tig0035450:+2357 Msa1454810:CDS
CCACCTGCTTCATCCCCTTA+CGG 0.496647 tig0035450:-2924 None:intergenic
ACAAGTGCATTGCAAAGCCT+TGG 0.497372 tig0035450:-2398 None:intergenic
TTGGTGAGACAGAGAACAAA+GGG 0.503937 tig0035450:-2265 None:intergenic
TTACTTTCCGCTTTGTCTTG+GGG 0.510969 tig0035450:-2015 None:intergenic
ACAAGATGATGAAACGTTCT+GGG 0.513788 tig0035450:+2759 Msa1454810:CDS
GTGAATTGTAGCCAATTCAA+TGG 0.516437 tig0035450:-3412 None:intergenic
ATTGAAGACAACAATTGCTA+TGG 0.521003 tig0035450:+2551 Msa1454810:CDS
TTGCACTGGACATATTACTT+AGG 0.522065 tig0035450:-3345 None:intergenic
ATTTGTTTGTGAGAGTGTGA+TGG 0.524245 tig0035450:+2199 Msa1454810:exon
ACAGAGAACAAAGGGAGCCT+TGG 0.527461 tig0035450:-2257 None:intergenic
GGGTTGAAGCTTACCCAGGT+AGG 0.527855 tig0035450:-2815 None:intergenic
TTTGTTTGTGAGAGTGTGAT+GGG 0.527929 tig0035450:+2200 Msa1454810:exon
CATGATTGCAATCATCCAAA+GGG 0.532004 tig0035450:-3214 None:intergenic
ATCATGGCAAGTCCCCTACC+TGG 0.532410 tig0035450:+2801 Msa1454810:CDS
TTTCGTTGGGAACAAGAACC+AGG 0.535652 tig0035450:+2371 Msa1454810:CDS
GTCACTACTGGGGTTAGTGA+AGG 0.542508 tig0035450:-2423 None:intergenic
CATCCGTAAGGGGATGAAGC+AGG 0.544671 tig0035450:+2921 Msa1454810:CDS
GGTTGAAGCTTACCCAGGTA+GGG 0.548146 tig0035450:-2814 None:intergenic
ACTCCACCACTTCAAACCTC+AGG 0.551998 tig0035450:+2338 Msa1454810:CDS
TCCTTATCAGTAGAAGAAGA+TGG 0.552504 tig0035450:-2707 None:intergenic
CAATTCTAAAAGATGGAGAC+CGG 0.554957 tig0035450:-2532 None:intergenic
ATGAGGACCCTGCTGCAAAA+GGG 0.555472 tig0035450:-2492 None:intergenic
TGCAATCATCCAAAGGGGAG+GGG 0.555503 tig0035450:-3208 None:intergenic
CTGCTTCATCCCCTTACGGA+TGG 0.555930 tig0035450:-2920 None:intergenic
TTATCAAAAGAGACAGTGGT+TGG 0.561874 tig0035450:+2624 Msa1454810:CDS
TCTTCTAGCCTCTCACATCA+TGG 0.562272 tig0035450:+2785 Msa1454810:CDS
AGCAGGTGGTTACACTTCCA+TGG 0.565557 tig0035450:+2938 Msa1454810:CDS
TTTGCAGACAACCATCCGTA+AGG 0.573822 tig0035450:+2909 Msa1454810:intron
GCAGTACTGTAAAAGAGTGT+GGG 0.578446 tig0035450:+3098 Msa1454810:three_prime_UTR
AATGGAATCCCCAGAGAGAA+CGG 0.582290 tig0035450:+2307 Msa1454810:CDS
TCACTACTGGGGTTAGTGAA+GGG 0.584112 tig0035450:-2422 None:intergenic
TTGCAATCATCCAAAGGGGA+GGG 0.585300 tig0035450:-3209 None:intergenic
TTGCAGACAACCATCCGTAA+GGG 0.587556 tig0035450:+2910 Msa1454810:intron
ATCCCCAGAGAGAACGGGGA+CGG 0.590463 tig0035450:+2313 Msa1454810:CDS
AGTGTTTGGAGCTTCCTCCA+AGG 0.599363 tig0035450:+2447 Msa1454810:CDS
ACTATGTTCATTATAGTGGA+AGG 0.599380 tig0035450:+3051 Msa1454810:three_prime_UTR
CACCGTCCCCGTTCTCTCTG+GGG 0.601477 tig0035450:-2315 None:intergenic
AGGCGAAGAAGTCAATAAAG+TGG 0.604743 tig0035450:-3325 None:intergenic
TGCTGCAAAAGGGTGGGTGA+GGG 0.606609 tig0035450:-2482 None:intergenic
GAGGGTACTACTAACAACCT+TGG 0.608997 tig0035450:-2464 None:intergenic
ACATGATTGCAATCATCCAA+AGG 0.609668 tig0035450:-3215 None:intergenic
GGTACTACTAACAACCTTGG+AGG 0.615771 tig0035450:-2461 None:intergenic
GTTGAAGCTTACCCAGGTAG+GGG 0.616247 tig0035450:-2813 None:intergenic
GAACCTGAGGTTTGAAGTGG+TGG 0.619285 tig0035450:-2341 None:intergenic
ATTCTTATCAAAAGAGACAG+TGG 0.624937 tig0035450:+2620 Msa1454810:CDS
TCATGGCAAGTCCCCTACCT+GGG 0.627898 tig0035450:+2802 Msa1454810:CDS
AACAAGAACCAGGAAAGCCA+AGG 0.628999 tig0035450:+2381 Msa1454810:CDS
CAGTACTGTAAAAGAGTGTG+GGG 0.630970 tig0035450:+3099 Msa1454810:three_prime_UTR
AAATGGAATAGAAAAGCAGA+AGG 0.632294 tig0035450:-3252 None:intergenic
TGCAGTACTGTAAAAGAGTG+TGG 0.633621 tig0035450:+3097 Msa1454810:three_prime_UTR
TGCAGACAACCATCCGTAAG+GGG 0.634249 tig0035450:+2911 Msa1454810:intron
TTGAAGAAGAAAGATGGGAG+TGG 0.637709 tig0035450:+2973 Msa1454810:CDS
AGCAAGAGAGCAGCATACCA+AGG 0.639698 tig0035450:+2240 Msa1454810:CDS
AAAAGAGATCAGGTCAGTGG+TGG 0.641682 tig0035450:+2671 Msa1454810:CDS
AGGACCCTGCTGCAAAAGGG+TGG 0.643526 tig0035450:-2489 None:intergenic
AATCTGTTGCTGGTTACATG+AGG 0.646820 tig0035450:-2509 None:intergenic
TGGAATCCCCAGAGAGAACG+GGG 0.647798 tig0035450:+2309 Msa1454810:CDS
GTCAAAAGAGATCAGGTCAG+TGG 0.649359 tig0035450:+2668 Msa1454810:CDS
GGGACTTGCCATGATGTGAG+AGG 0.660529 tig0035450:-2793 None:intergenic
ATTGCAATCATCCAAAGGGG+AGG 0.665014 tig0035450:-3210 None:intergenic
ACGAAATGGTACTGAACCTG+AGG 0.668546 tig0035450:-2354 None:intergenic
ATTGGTGAGACAGAGAACAA+AGG 0.669249 tig0035450:-2266 None:intergenic
TATTGCACCCCAAGACAAAG+CGG 0.682956 tig0035450:+2008 None:intergenic
TAGAAGAAGATGGAAAGACA+TGG 0.687112 tig0035450:-2697 None:intergenic
CCGTAAGGGGATGAAGCAGG+TGG 0.695960 tig0035450:+2924 Msa1454810:CDS
GGACCCTGCTGCAAAAGGGT+GGG 0.699332 tig0035450:-2488 None:intergenic
ACTGAACCTGAGGTTTGAAG+TGG 0.699731 tig0035450:-2344 None:intergenic
ATGATTGCAATCATCCAAAG+GGG 0.716629 tig0035450:-3213 None:intergenic
CCAAACACTTGTCACTACTG+GGG 0.720868 tig0035450:-2433 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTATTAAAATGCAAA+TGG + tig0035450:2871-2890 Msa1454810:intron 10.0%
!! AATTATTATTAAAATGCAAA+TGG + tig0035450:2871-2890 Msa1454810:intron 10.0%
!! AAATGATATAAAAGATCATT+TGG - tig0035450:2135-2154 None:intergenic 15.0%
!! CAGTAAGATAATAATAATAA+AGG - tig0035450:2160-2179 None:intergenic 15.0%
!! AAATGATATAAAAGATCATT+TGG - tig0035450:2135-2154 None:intergenic 15.0%
!! CAGTAAGATAATAATAATAA+AGG - tig0035450:2160-2179 None:intergenic 15.0%
!!! TGCATTTGAATTTTCATTAT+TGG - tig0035450:2287-2306 None:intergenic 20.0%
!! AATGAAAATTCAAATGCAAA+TGG + tig0035450:2289-2308 Msa1454810:CDS 20.0%
!! ATTACTAATTTCTACTAAGT+AGG - tig0035450:3176-3195 None:intergenic 20.0%
!!! TGCATTTGAATTTTCATTAT+TGG - tig0035450:2287-2306 None:intergenic 20.0%
!! AATGAAAATTCAAATGCAAA+TGG + tig0035450:2289-2308 Msa1454810:CDS 20.0%
!! ATTACTAATTTCTACTAAGT+AGG - tig0035450:3176-3195 None:intergenic 20.0%
! TGAGTTTGATATAACTGAAT+TGG - tig0035450:2104-2123 None:intergenic 25.0%
!! TTGTCTTCAATTCTAAAAGA+TGG - tig0035450:2542-2561 None:intergenic 25.0%
! AAAAAGTTGAAGAAGAAAGA+TGG + tig0035450:2967-2986 Msa1454810:CDS 25.0%
! AAAAGTTGAAGAAGAAAGAT+GGG + tig0035450:2968-2987 Msa1454810:CDS 25.0%
! AAATCTATTCTCAAAGCTTA+AGG - tig0035450:2999-3018 None:intergenic 25.0%
! AGAAACTATGTTCATTATAG+TGG + tig0035450:3047-3066 Msa1454810:three_prime_UTR 25.0%
!!! ACATTCAACTTGTTGTTATT+TGG - tig0035450:3302-3321 None:intergenic 25.0%
! TGAGTTTGATATAACTGAAT+TGG - tig0035450:2104-2123 None:intergenic 25.0%
!! TTGTCTTCAATTCTAAAAGA+TGG - tig0035450:2542-2561 None:intergenic 25.0%
! AAAAAGTTGAAGAAGAAAGA+TGG + tig0035450:2967-2986 Msa1454810:CDS 25.0%
! AAAAGTTGAAGAAGAAAGAT+GGG + tig0035450:2968-2987 Msa1454810:CDS 25.0%
! AAATCTATTCTCAAAGCTTA+AGG - tig0035450:2999-3018 None:intergenic 25.0%
! AGAAACTATGTTCATTATAG+TGG + tig0035450:3047-3066 Msa1454810:three_prime_UTR 25.0%
!!! ACATTCAACTTGTTGTTATT+TGG - tig0035450:3302-3321 None:intergenic 25.0%
! GAAAGTGTGGAAATATGATT+AGG - tig0035450:2067-2086 None:intergenic 30.0%
ATTGAAGACAACAATTGCTA+TGG + tig0035450:2551-2570 Msa1454810:CDS 30.0%
ATTCTTATCAAAAGAGACAG+TGG + tig0035450:2620-2639 Msa1454810:CDS 30.0%
GAAAAATGTCAAAAGAGATC+AGG + tig0035450:2661-2680 Msa1454810:CDS 30.0%
TTCCACACAGAATGAAAAAA+GGG - tig0035450:2838-2857 None:intergenic 30.0%
ATTCCACACAGAATGAAAAA+AGG - tig0035450:2839-2858 None:intergenic 30.0%
! ACTATGTTCATTATAGTGGA+AGG + tig0035450:3051-3070 Msa1454810:three_prime_UTR 30.0%
AAATGGAATAGAAAAGCAGA+AGG - tig0035450:3255-3274 None:intergenic 30.0%
AAAGACAAGTACTAAGCAAA+TGG - tig0035450:3272-3291 None:intergenic 30.0%
TCCAGTGCAAATGATATTTA+TGG + tig0035450:3358-3377 Msa1454810:three_prime_UTR 30.0%
TCCATAAATATCATTTGCAC+TGG - tig0035450:3362-3381 None:intergenic 30.0%
! GAAAGTGTGGAAATATGATT+AGG - tig0035450:2067-2086 None:intergenic 30.0%
ATTGAAGACAACAATTGCTA+TGG + tig0035450:2551-2570 Msa1454810:CDS 30.0%
ATTCTTATCAAAAGAGACAG+TGG + tig0035450:2620-2639 Msa1454810:CDS 30.0%
GAAAAATGTCAAAAGAGATC+AGG + tig0035450:2661-2680 Msa1454810:CDS 30.0%
TTCCACACAGAATGAAAAAA+GGG - tig0035450:2838-2857 None:intergenic 30.0%
ATTCCACACAGAATGAAAAA+AGG - tig0035450:2839-2858 None:intergenic 30.0%
! ACTATGTTCATTATAGTGGA+AGG + tig0035450:3051-3070 Msa1454810:three_prime_UTR 30.0%
AAATGGAATAGAAAAGCAGA+AGG - tig0035450:3255-3274 None:intergenic 30.0%
AAAGACAAGTACTAAGCAAA+TGG - tig0035450:3272-3291 None:intergenic 30.0%
TCCAGTGCAAATGATATTTA+TGG + tig0035450:3358-3377 Msa1454810:three_prime_UTR 30.0%
TCCATAAATATCATTTGCAC+TGG - tig0035450:3362-3381 None:intergenic 30.0%
TATTTCCACACTTTCCTCTT+TGG + tig0035450:2072-2091 Msa1454810:five_prime_UTR 35.0%
! TGAATTGGATTTTGCCAAAG+AGG - tig0035450:2089-2108 None:intergenic 35.0%
! ATTTGTTTGTGAGAGTGTGA+TGG + tig0035450:2199-2218 Msa1454810:exon 35.0%
! TTTGTTTGTGAGAGTGTGAT+GGG + tig0035450:2200-2219 Msa1454810:exon 35.0%
! CAATTCTAAAAGATGGAGAC+CGG - tig0035450:2535-2554 None:intergenic 35.0%
TTATCAAAAGAGACAGTGGT+TGG + tig0035450:2624-2643 Msa1454810:CDS 35.0%
TAGAAGAAGATGGAAAGACA+TGG - tig0035450:2700-2719 None:intergenic 35.0%
TCCATCTTCTTCTACTGATA+AGG + tig0035450:2706-2725 Msa1454810:CDS 35.0%
TCCTTATCAGTAGAAGAAGA+TGG - tig0035450:2710-2729 None:intergenic 35.0%
TCTTCTACTGATAAGGATAC+TGG + tig0035450:2713-2732 Msa1454810:CDS 35.0%
AACAAGATGATGAAACGTTC+TGG + tig0035450:2758-2777 Msa1454810:CDS 35.0%
ACAAGATGATGAAACGTTCT+GGG + tig0035450:2759-2778 Msa1454810:CDS 35.0%
!!! AACCCTTTTTTCATTCTGTG+TGG + tig0035450:2833-2852 Msa1454810:intron 35.0%
! TTCAACTTTTTGCTTCTCCA+TGG - tig0035450:2958-2977 None:intergenic 35.0%
!!! GCTTTGAGAATAGATTTTGC+TGG + tig0035450:3002-3021 Msa1454810:exon 35.0%
! TAGTAGTCTCTGTTTTCTAG+TGG + tig0035450:3134-3153 Msa1454810:three_prime_UTR 35.0%
!! AGTAGTCTCTGTTTTCTAGT+GGG + tig0035450:3135-3154 Msa1454810:three_prime_UTR 35.0%
ATGATTGCAATCATCCAAAG+GGG - tig0035450:3216-3235 None:intergenic 35.0%
CATGATTGCAATCATCCAAA+GGG - tig0035450:3217-3236 None:intergenic 35.0%
ACATGATTGCAATCATCCAA+AGG - tig0035450:3218-3237 None:intergenic 35.0%
TTGCACTGGACATATTACTT+AGG - tig0035450:3348-3367 None:intergenic 35.0%
TATTTCCACACTTTCCTCTT+TGG + tig0035450:2072-2091 Msa1454810:five_prime_UTR 35.0%
! TGAATTGGATTTTGCCAAAG+AGG - tig0035450:2089-2108 None:intergenic 35.0%
! ATTTGTTTGTGAGAGTGTGA+TGG + tig0035450:2199-2218 Msa1454810:exon 35.0%
! TTTGTTTGTGAGAGTGTGAT+GGG + tig0035450:2200-2219 Msa1454810:exon 35.0%
! CAATTCTAAAAGATGGAGAC+CGG - tig0035450:2535-2554 None:intergenic 35.0%
TTATCAAAAGAGACAGTGGT+TGG + tig0035450:2624-2643 Msa1454810:CDS 35.0%
TAGAAGAAGATGGAAAGACA+TGG - tig0035450:2700-2719 None:intergenic 35.0%
TCCATCTTCTTCTACTGATA+AGG + tig0035450:2706-2725 Msa1454810:CDS 35.0%
TCCTTATCAGTAGAAGAAGA+TGG - tig0035450:2710-2729 None:intergenic 35.0%
TCTTCTACTGATAAGGATAC+TGG + tig0035450:2713-2732 Msa1454810:CDS 35.0%
AACAAGATGATGAAACGTTC+TGG + tig0035450:2758-2777 Msa1454810:CDS 35.0%
ACAAGATGATGAAACGTTCT+GGG + tig0035450:2759-2778 Msa1454810:CDS 35.0%
!!! AACCCTTTTTTCATTCTGTG+TGG + tig0035450:2833-2852 Msa1454810:intron 35.0%
! TTCAACTTTTTGCTTCTCCA+TGG - tig0035450:2958-2977 None:intergenic 35.0%
!!! GCTTTGAGAATAGATTTTGC+TGG + tig0035450:3002-3021 Msa1454810:exon 35.0%
! TAGTAGTCTCTGTTTTCTAG+TGG + tig0035450:3134-3153 Msa1454810:three_prime_UTR 35.0%
!! AGTAGTCTCTGTTTTCTAGT+GGG + tig0035450:3135-3154 Msa1454810:three_prime_UTR 35.0%
ATGATTGCAATCATCCAAAG+GGG - tig0035450:3216-3235 None:intergenic 35.0%
CATGATTGCAATCATCCAAA+GGG - tig0035450:3217-3236 None:intergenic 35.0%
ACATGATTGCAATCATCCAA+AGG - tig0035450:3218-3237 None:intergenic 35.0%
TTGCACTGGACATATTACTT+AGG - tig0035450:3348-3367 None:intergenic 35.0%
!! TGTTACTTTCCGCTTTGTCT+TGG - tig0035450:2020-2039 None:intergenic 40.0%
! TTTTGCCAAAGAGGAAAGTG+TGG - tig0035450:2080-2099 None:intergenic 40.0%
TTGGTGAGACAGAGAACAAA+GGG - tig0035450:2268-2287 None:intergenic 40.0%
ATTGGTGAGACAGAGAACAA+AGG - tig0035450:2269-2288 None:intergenic 40.0%
AGGTTCAGTACCATTTCGTT+GGG + tig0035450:2358-2377 Msa1454810:CDS 40.0%
TCCAAACACTTGTCACTACT+GGG - tig0035450:2437-2456 None:intergenic 40.0%
! AATCTGTTGCTGGTTACATG+AGG - tig0035450:2512-2531 None:intergenic 40.0%
ATGTAACCAGCAACAGATTC+CGG + tig0035450:2513-2532 Msa1454810:CDS 40.0%
!! TCTTCGTTCAGTACTGATAG+AGG + tig0035450:2575-2594 Msa1454810:CDS 40.0%
!! AGTACTGATAGAGGCTTACT+TGG + tig0035450:2584-2603 Msa1454810:CDS 40.0%
! AAAAGAGACAGTGGTTGGTT+TGG + tig0035450:2629-2648 Msa1454810:CDS 40.0%
GTTGTTGTCTGCAATGTCAA+TGG - tig0035450:2741-2760 None:intergenic 40.0%
TTGAAGAAGAAAGATGGGAG+TGG + tig0035450:2973-2992 Msa1454810:CDS 40.0%
CAGTACTGCAACAAATTAGC+TGG - tig0035450:3087-3106 None:intergenic 40.0%
TGCAGTACTGTAAAAGAGTG+TGG + tig0035450:3097-3116 Msa1454810:three_prime_UTR 40.0%
! GCAGTACTGTAAAAGAGTGT+GGG + tig0035450:3098-3117 Msa1454810:three_prime_UTR 40.0%
! CAGTACTGTAAAAGAGTGTG+GGG + tig0035450:3099-3118 Msa1454810:three_prime_UTR 40.0%
!! GTAGTCTCTGTTTTCTAGTG+GGG + tig0035450:3136-3155 Msa1454810:three_prime_UTR 40.0%
AGGCGAAGAAGTCAATAAAG+TGG - tig0035450:3328-3347 None:intergenic 40.0%
AATGGCGTTACGATAGAACT+TGG - tig0035450:3397-3416 None:intergenic 40.0%
CTATCGTAACGCCATTGAAT+TGG + tig0035450:3401-3420 Msa1454810:three_prime_UTR 40.0%
!! TGTTACTTTCCGCTTTGTCT+TGG - tig0035450:2020-2039 None:intergenic 40.0%
! TTTTGCCAAAGAGGAAAGTG+TGG - tig0035450:2080-2099 None:intergenic 40.0%
TTGGTGAGACAGAGAACAAA+GGG - tig0035450:2268-2287 None:intergenic 40.0%
ATTGGTGAGACAGAGAACAA+AGG - tig0035450:2269-2288 None:intergenic 40.0%
AGGTTCAGTACCATTTCGTT+GGG + tig0035450:2358-2377 Msa1454810:CDS 40.0%
TCCAAACACTTGTCACTACT+GGG - tig0035450:2437-2456 None:intergenic 40.0%
! AATCTGTTGCTGGTTACATG+AGG - tig0035450:2512-2531 None:intergenic 40.0%
ATGTAACCAGCAACAGATTC+CGG + tig0035450:2513-2532 Msa1454810:CDS 40.0%
!! TCTTCGTTCAGTACTGATAG+AGG + tig0035450:2575-2594 Msa1454810:CDS 40.0%
!! AGTACTGATAGAGGCTTACT+TGG + tig0035450:2584-2603 Msa1454810:CDS 40.0%
! AAAAGAGACAGTGGTTGGTT+TGG + tig0035450:2629-2648 Msa1454810:CDS 40.0%
GTTGTTGTCTGCAATGTCAA+TGG - tig0035450:2741-2760 None:intergenic 40.0%
TTGAAGAAGAAAGATGGGAG+TGG + tig0035450:2973-2992 Msa1454810:CDS 40.0%
CAGTACTGCAACAAATTAGC+TGG - tig0035450:3087-3106 None:intergenic 40.0%
TGCAGTACTGTAAAAGAGTG+TGG + tig0035450:3097-3116 Msa1454810:three_prime_UTR 40.0%
! GCAGTACTGTAAAAGAGTGT+GGG + tig0035450:3098-3117 Msa1454810:three_prime_UTR 40.0%
! CAGTACTGTAAAAGAGTGTG+GGG + tig0035450:3099-3118 Msa1454810:three_prime_UTR 40.0%
!! GTAGTCTCTGTTTTCTAGTG+GGG + tig0035450:3136-3155 Msa1454810:three_prime_UTR 40.0%
AGGCGAAGAAGTCAATAAAG+TGG - tig0035450:3328-3347 None:intergenic 40.0%
AATGGCGTTACGATAGAACT+TGG - tig0035450:3397-3416 None:intergenic 40.0%
CTATCGTAACGCCATTGAAT+TGG + tig0035450:3401-3420 Msa1454810:three_prime_UTR 40.0%
AATGGAATCCCCAGAGAGAA+CGG + tig0035450:2307-2326 Msa1454810:CDS 45.0%
ACTGAACCTGAGGTTTGAAG+TGG - tig0035450:2347-2366 None:intergenic 45.0%
! ACGAAATGGTACTGAACCTG+AGG - tig0035450:2357-2376 None:intergenic 45.0%
CAGGTTCAGTACCATTTCGT+TGG + tig0035450:2357-2376 Msa1454810:CDS 45.0%
GGTTCTTGTTCCCAACGAAA+TGG - tig0035450:2371-2390 None:intergenic 45.0%
TTTCGTTGGGAACAAGAACC+AGG + tig0035450:2371-2390 Msa1454810:CDS 45.0%
AACAAGAACCAGGAAAGCCA+AGG + tig0035450:2381-2400 Msa1454810:CDS 45.0%
ACAAGTGCATTGCAAAGCCT+TGG - tig0035450:2401-2420 None:intergenic 45.0%
! TCACTACTGGGGTTAGTGAA+GGG - tig0035450:2425-2444 None:intergenic 45.0%
CCAAACACTTGTCACTACTG+GGG - tig0035450:2436-2455 None:intergenic 45.0%
CTCCAAACACTTGTCACTAC+TGG - tig0035450:2438-2457 None:intergenic 45.0%
GGTACTACTAACAACCTTGG+AGG - tig0035450:2464-2483 None:intergenic 45.0%
!! GAGGGTACTACTAACAACCT+TGG - tig0035450:2467-2486 None:intergenic 45.0%
!! ACAGTGGTTGGTTTGGTTCT+TGG + tig0035450:2636-2655 Msa1454810:CDS 45.0%
GTCAAAAGAGATCAGGTCAG+TGG + tig0035450:2668-2687 Msa1454810:CDS 45.0%
AAAAGAGATCAGGTCAGTGG+TGG + tig0035450:2671-2690 Msa1454810:CDS 45.0%
TCTTCTAGCCTCTCACATCA+TGG + tig0035450:2785-2804 Msa1454810:CDS 45.0%
AAAAGGGTTGAAGCTTACCC+AGG - tig0035450:2822-2841 None:intergenic 45.0%
TTTGCAGACAACCATCCGTA+AGG + tig0035450:2909-2928 Msa1454810:intron 45.0%
TTGCAGACAACCATCCGTAA+GGG + tig0035450:2910-2929 Msa1454810:intron 45.0%
TTGCAATCATCCAAAGGGGA+GGG - tig0035450:3212-3231 None:intergenic 45.0%
ATTGCAATCATCCAAAGGGG+AGG - tig0035450:3213-3232 None:intergenic 45.0%
AATGGAATCCCCAGAGAGAA+CGG + tig0035450:2307-2326 Msa1454810:CDS 45.0%
ACTGAACCTGAGGTTTGAAG+TGG - tig0035450:2347-2366 None:intergenic 45.0%
! ACGAAATGGTACTGAACCTG+AGG - tig0035450:2357-2376 None:intergenic 45.0%
CAGGTTCAGTACCATTTCGT+TGG + tig0035450:2357-2376 Msa1454810:CDS 45.0%
GGTTCTTGTTCCCAACGAAA+TGG - tig0035450:2371-2390 None:intergenic 45.0%
TTTCGTTGGGAACAAGAACC+AGG + tig0035450:2371-2390 Msa1454810:CDS 45.0%
AACAAGAACCAGGAAAGCCA+AGG + tig0035450:2381-2400 Msa1454810:CDS 45.0%
ACAAGTGCATTGCAAAGCCT+TGG - tig0035450:2401-2420 None:intergenic 45.0%
! TCACTACTGGGGTTAGTGAA+GGG - tig0035450:2425-2444 None:intergenic 45.0%
CCAAACACTTGTCACTACTG+GGG - tig0035450:2436-2455 None:intergenic 45.0%
CTCCAAACACTTGTCACTAC+TGG - tig0035450:2438-2457 None:intergenic 45.0%
GGTACTACTAACAACCTTGG+AGG - tig0035450:2464-2483 None:intergenic 45.0%
!! GAGGGTACTACTAACAACCT+TGG - tig0035450:2467-2486 None:intergenic 45.0%
!! ACAGTGGTTGGTTTGGTTCT+TGG + tig0035450:2636-2655 Msa1454810:CDS 45.0%
GTCAAAAGAGATCAGGTCAG+TGG + tig0035450:2668-2687 Msa1454810:CDS 45.0%
AAAAGAGATCAGGTCAGTGG+TGG + tig0035450:2671-2690 Msa1454810:CDS 45.0%
TCTTCTAGCCTCTCACATCA+TGG + tig0035450:2785-2804 Msa1454810:CDS 45.0%
AAAAGGGTTGAAGCTTACCC+AGG - tig0035450:2822-2841 None:intergenic 45.0%
TTTGCAGACAACCATCCGTA+AGG + tig0035450:2909-2928 Msa1454810:intron 45.0%
TTGCAGACAACCATCCGTAA+GGG + tig0035450:2910-2929 Msa1454810:intron 45.0%
TTGCAATCATCCAAAGGGGA+GGG - tig0035450:3212-3231 None:intergenic 45.0%
ATTGCAATCATCCAAAGGGG+AGG - tig0035450:3213-3232 None:intergenic 45.0%
AGCAAGAGAGCAGCATACCA+AGG + tig0035450:2240-2259 Msa1454810:CDS 50.0%
ACAGAGAACAAAGGGAGCCT+TGG - tig0035450:2260-2279 None:intergenic 50.0%
ATGGAATCCCCAGAGAGAAC+GGG + tig0035450:2308-2327 Msa1454810:CDS 50.0%
ACTCCACCACTTCAAACCTC+AGG + tig0035450:2338-2357 Msa1454810:CDS 50.0%
! GAACCTGAGGTTTGAAGTGG+TGG - tig0035450:2344-2363 None:intergenic 50.0%
! TTGCAAAGCCTTGGCTTTCC+TGG - tig0035450:2392-2411 None:intergenic 50.0%
! GTCACTACTGGGGTTAGTGA+AGG - tig0035450:2426-2445 None:intergenic 50.0%
CCCCAGTAGTGACAAGTGTT+TGG + tig0035450:2433-2452 Msa1454810:CDS 50.0%
! AGTGTTTGGAGCTTCCTCCA+AGG + tig0035450:2447-2466 Msa1454810:CDS 50.0%
ATGAGGACCCTGCTGCAAAA+GGG - tig0035450:2495-2514 None:intergenic 50.0%
!! GTGGTTGGTTTGGTTCTTGG+AGG + tig0035450:2639-2658 Msa1454810:CDS 50.0%
GTTGAAGCTTACCCAGGTAG+GGG - tig0035450:2816-2835 None:intergenic 50.0%
GGTTGAAGCTTACCCAGGTA+GGG - tig0035450:2817-2836 None:intergenic 50.0%
TGCAGACAACCATCCGTAAG+GGG + tig0035450:2911-2930 Msa1454810:intron 50.0%
AGCAGGTGGTTACACTTCCA+TGG + tig0035450:2938-2957 Msa1454810:CDS 50.0%
ACAATTACTCCCCTCCCCTT+TGG + tig0035450:3199-3218 Msa1454810:three_prime_UTR 50.0%
TGCAATCATCCAAAGGGGAG+GGG - tig0035450:3211-3230 None:intergenic 50.0%
AGCAAGAGAGCAGCATACCA+AGG + tig0035450:2240-2259 Msa1454810:CDS 50.0%
ACAGAGAACAAAGGGAGCCT+TGG - tig0035450:2260-2279 None:intergenic 50.0%
ATGGAATCCCCAGAGAGAAC+GGG + tig0035450:2308-2327 Msa1454810:CDS 50.0%
ACTCCACCACTTCAAACCTC+AGG + tig0035450:2338-2357 Msa1454810:CDS 50.0%
! GAACCTGAGGTTTGAAGTGG+TGG - tig0035450:2344-2363 None:intergenic 50.0%
! TTGCAAAGCCTTGGCTTTCC+TGG - tig0035450:2392-2411 None:intergenic 50.0%
! GTCACTACTGGGGTTAGTGA+AGG - tig0035450:2426-2445 None:intergenic 50.0%
CCCCAGTAGTGACAAGTGTT+TGG + tig0035450:2433-2452 Msa1454810:CDS 50.0%
! AGTGTTTGGAGCTTCCTCCA+AGG + tig0035450:2447-2466 Msa1454810:CDS 50.0%
ATGAGGACCCTGCTGCAAAA+GGG - tig0035450:2495-2514 None:intergenic 50.0%
!! GTGGTTGGTTTGGTTCTTGG+AGG + tig0035450:2639-2658 Msa1454810:CDS 50.0%
GTTGAAGCTTACCCAGGTAG+GGG - tig0035450:2816-2835 None:intergenic 50.0%
GGTTGAAGCTTACCCAGGTA+GGG - tig0035450:2817-2836 None:intergenic 50.0%
TGCAGACAACCATCCGTAAG+GGG + tig0035450:2911-2930 Msa1454810:intron 50.0%
AGCAGGTGGTTACACTTCCA+TGG + tig0035450:2938-2957 Msa1454810:CDS 50.0%
ACAATTACTCCCCTCCCCTT+TGG + tig0035450:3199-3218 Msa1454810:three_prime_UTR 50.0%
TGCAATCATCCAAAGGGGAG+GGG - tig0035450:3211-3230 None:intergenic 50.0%
TGGAATCCCCAGAGAGAACG+GGG + tig0035450:2309-2328 Msa1454810:CDS 55.0%
TGCTGCAAAAGGGTGGGTGA+GGG - tig0035450:2485-2504 None:intergenic 55.0%
! TCACCCACCCTTTTGCAGCA+GGG + tig0035450:2485-2504 Msa1454810:CDS 55.0%
CATGAGGACCCTGCTGCAAA+AGG - tig0035450:2496-2515 None:intergenic 55.0%
TGGAGACCGGAATCTGTTGC+TGG - tig0035450:2522-2541 None:intergenic 55.0%
GGGACTTGCCATGATGTGAG+AGG - tig0035450:2796-2815 None:intergenic 55.0%
ATCATGGCAAGTCCCCTACC+TGG + tig0035450:2801-2820 Msa1454810:CDS 55.0%
TCATGGCAAGTCCCCTACCT+GGG + tig0035450:2802-2821 Msa1454810:CDS 55.0%
GGGTTGAAGCTTACCCAGGT+AGG - tig0035450:2818-2837 None:intergenic 55.0%
CTGCTTCATCCCCTTACGGA+TGG - tig0035450:2923-2942 None:intergenic 55.0%
CATCCGTAAGGGGATGAAGC+AGG + tig0035450:2921-2940 Msa1454810:CDS 55.0%
CCACCTGCTTCATCCCCTTA+CGG - tig0035450:2927-2946 None:intergenic 55.0%
TGGAATCCCCAGAGAGAACG+GGG + tig0035450:2309-2328 Msa1454810:CDS 55.0%
TGCTGCAAAAGGGTGGGTGA+GGG - tig0035450:2485-2504 None:intergenic 55.0%
! TCACCCACCCTTTTGCAGCA+GGG + tig0035450:2485-2504 Msa1454810:CDS 55.0%
CATGAGGACCCTGCTGCAAA+AGG - tig0035450:2496-2515 None:intergenic 55.0%
TGGAGACCGGAATCTGTTGC+TGG - tig0035450:2522-2541 None:intergenic 55.0%
GGGACTTGCCATGATGTGAG+AGG - tig0035450:2796-2815 None:intergenic 55.0%
ATCATGGCAAGTCCCCTACC+TGG + tig0035450:2801-2820 Msa1454810:CDS 55.0%
TCATGGCAAGTCCCCTACCT+GGG + tig0035450:2802-2821 Msa1454810:CDS 55.0%
GGGTTGAAGCTTACCCAGGT+AGG - tig0035450:2818-2837 None:intergenic 55.0%
CTGCTTCATCCCCTTACGGA+TGG - tig0035450:2923-2942 None:intergenic 55.0%
CATCCGTAAGGGGATGAAGC+AGG + tig0035450:2921-2940 Msa1454810:CDS 55.0%
CCACCTGCTTCATCCCCTTA+CGG - tig0035450:2927-2946 None:intergenic 55.0%
ATCCCCAGAGAGAACGGGGA+CGG + tig0035450:2313-2332 Msa1454810:CDS 60.0%
TCACCGTCCCCGTTCTCTCT+GGG - tig0035450:2319-2338 None:intergenic 60.0%
CTGCTGCAAAAGGGTGGGTG+AGG - tig0035450:2486-2505 None:intergenic 60.0%
! CTCACCCACCCTTTTGCAGC+AGG + tig0035450:2484-2503 Msa1454810:CDS 60.0%
GGACCCTGCTGCAAAAGGGT+GGG - tig0035450:2491-2510 None:intergenic 60.0%
AGGACCCTGCTGCAAAAGGG+TGG - tig0035450:2492-2511 None:intergenic 60.0%
CCGTAAGGGGATGAAGCAGG+TGG + tig0035450:2924-2943 Msa1454810:CDS 60.0%
ATCCCCAGAGAGAACGGGGA+CGG + tig0035450:2313-2332 Msa1454810:CDS 60.0%
TCACCGTCCCCGTTCTCTCT+GGG - tig0035450:2319-2338 None:intergenic 60.0%
CTGCTGCAAAAGGGTGGGTG+AGG - tig0035450:2486-2505 None:intergenic 60.0%
! CTCACCCACCCTTTTGCAGC+AGG + tig0035450:2484-2503 Msa1454810:CDS 60.0%
GGACCCTGCTGCAAAAGGGT+GGG - tig0035450:2491-2510 None:intergenic 60.0%
AGGACCCTGCTGCAAAAGGG+TGG - tig0035450:2492-2511 None:intergenic 60.0%
CCGTAAGGGGATGAAGCAGG+TGG + tig0035450:2924-2943 Msa1454810:CDS 60.0%
CACCGTCCCCGTTCTCTCTG+GGG - tig0035450:2318-2337 None:intergenic 65.0%
GTCACCGTCCCCGTTCTCTC+TGG - tig0035450:2320-2339 None:intergenic 65.0%
CACCGTCCCCGTTCTCTCTG+GGG - tig0035450:2318-2337 None:intergenic 65.0%
GTCACCGTCCCCGTTCTCTC+TGG - tig0035450:2320-2339 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0035450 gene 2017 3425 2017 ID=Msa1454810;Name=Msa1454810
tig0035450 mRNA 2017 3425 2017 ID=Msa1454810-mRNA-1;Parent=Msa1454810;Name=Msa1454810-mRNA-1;_AED=0.02;_eAED=0.02;_QI=201|1|1|1|1|1|2|417|232
tig0035450 exon 2017 2823 2017 ID=Msa1454810-mRNA-1:exon:19942;Parent=Msa1454810-mRNA-1
tig0035450 exon 2916 3425 2916 ID=Msa1454810-mRNA-1:exon:19943;Parent=Msa1454810-mRNA-1
tig0035450 five_prime_UTR 2017 2217 2017 ID=Msa1454810-mRNA-1:five_prime_utr;Parent=Msa1454810-mRNA-1
tig0035450 CDS 2218 2823 2218 ID=Msa1454810-mRNA-1:cds;Parent=Msa1454810-mRNA-1
tig0035450 CDS 2916 3008 2916 ID=Msa1454810-mRNA-1:cds;Parent=Msa1454810-mRNA-1
tig0035450 three_prime_UTR 3009 3425 3009 ID=Msa1454810-mRNA-1:three_prime_utr;Parent=Msa1454810-mRNA-1
Gene Sequence

>Msa1454810

ATGGGTTCTGAAGCAAAGACAGAGCAAGAGAGCAGCATACCAAGGCTCCCTTTGTTCTCTGTCTCACCAATAATGAAAATTCAAATGCAAATGGAATCCCCAGAGAGAACGGGGACGGTGACTCCACCACTTCAAACCTCAGGTTCAGTACCATTTCGTTGGGAACAAGAACCAGGAAAGCCAAGGCTTTGCAATGCACTTGTGCCCTTCACTAACCCCAGTAGTGACAAGTGTTTGGAGCTTCCTCCAAGGTTGTTAGTAGTACCCTCACCCACCCTTTTGCAGCAGGGTCCTCATGTAACCAGCAACAGATTCCGGTCTCCATCTTTTAGAATTGAAGACAACAATTGCTATGGTTCTTCGTTCAGTACTGATAGAGGCTTACTTGGTAGTACTATGAATATTCTTATCAAAAGAGACAGTGGTTGGTTTGGTTCTTGGAGGAAAAATGTCAAAAGAGATCAGGTCAGTGGTGGTAGCCATGTCTTTCCATCTTCTTCTACTGATAAGGATACTGGAACCATTGACATTGCAGACAACAACAAGATGATGAAACGTTCTGGGAGTTCTTCTAGCCTCTCACATCATGGCAAGTCCCCTACCTGGACAACCATCCGTAAGGGGATGAAGCAGGTGGTTACACTTCCATGGAGAAGCAAAAAGTTGAAGAAGAAAGATGGGAGTGGCCTTAAGCTTTGA
Protein sequence

>Msa1454810

MGSEAKTEQESSIPRLPLFSVSPIMKIQMQMESPERTGTVTPPLQTSGSVPFRWEQEPGKPRLCNALVPFTNPSSDKCLELPPRLLVVPSPTLLQQGPHVTSNRFRSPSFRIEDNNCYGSSFSTDRGLLGSTMNILIKRDSGWFGSWRKNVKRDQVSGGSHVFPSSSTDKDTGTIDIADNNKMMKRSGSSSSLSHHGKSPTWTTIRKGMKQVVTLPWRSKKLKKKDGSGLKL*