AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1454820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454820 MtrunA17_Chr7g0275871 98.413 63 1 0 1 63 1 63 3.13e-40 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1454820 AT2G46540.1 69.841 63 19 0 1 63 1 63 1.27e-26 92.0

Find 36 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATATAAAATGAAGTCTT+TGG 0.230572 tig0035450:+11022 None:intergenic
TAGTTGTGCAAGGGACATTT+TGG 0.235709 tig0035450:-12492 Msa1454820:five_prime_UTR
TTGGGATACAAGGGAATAAT+TGG 0.267204 tig0035450:-11155 Msa1454820:three_prime_UTR
ATGTGACAAAATTGATGTTT+AGG 0.286631 tig0035450:+12437 None:intergenic
TTTGTCACATGTAACAATTT+TGG 0.291842 tig0035450:-12424 Msa1454820:five_prime_UTR
ATCGAAATCGAAATCGAAAA+TGG 0.295817 tig0035450:-12345 Msa1454820:exon
ATGAAGTCTTTGGATTAATC+TGG 0.300191 tig0035450:+11032 None:intergenic
TTATATTTAAGTTAAGTCTT+TGG 0.339866 tig0035450:-11008 Msa1454820:three_prime_UTR
TCTTGTCTCATCGCTGGCTT+TGG 0.357510 tig0035450:-12269 Msa1454820:intron
TTGTTCCTCTAGTGACAATA+TGG 0.360388 tig0035450:-10920 Msa1454820:three_prime_UTR
ATATCGTTGCAGGTGGTTGC+AGG 0.366532 tig0035450:-11207 Msa1454820:intron
AGACAAGAAGCAGCGACAAC+TGG 0.459618 tig0035450:+12284 None:intergenic
GCTGCTTCTTGTCTCATCGC+TGG 0.475454 tig0035450:-12275 Msa1454820:CDS
AACTGATAGATTGTCACAAA+TGG 0.480273 tig0035450:+11055 None:intergenic
AGCATAGGCCTCACAAAAGC+AGG 0.483396 tig0035450:+11708 None:intergenic
TCACAAAAGCAGGAAGGAAA+AGG 0.487675 tig0035450:+11718 None:intergenic
GCTGATTCACTTGGGATACA+AGG 0.487925 tig0035450:-11165 Msa1454820:three_prime_UTR
TAGTAGAGGGAAAATACATC+AGG 0.506350 tig0035450:+10961 None:intergenic
AGCAGGTTGAGGTGGTTGCT+CGG 0.511614 tig0035450:+11668 None:intergenic
GAAATCGAAAATGGCTGTGA+TGG 0.524123 tig0035450:-12336 Msa1454820:exon
CAGGTGGTTGCAGGTATGAC+TGG 0.527716 tig0035450:-11198 Msa1454820:CDS
CACAGAACAGAACAGAAGAA+AGG 0.538975 tig0035450:-12379 Msa1454820:five_prime_UTR
GTTCGATATGTGTCCAGCAT+AGG 0.541431 tig0035450:+11693 None:intergenic
ACATCGCTCAAAGCAGGTTG+AGG 0.547023 tig0035450:+11657 None:intergenic
AAACTCACATCGCTCAAAGC+AGG 0.558963 tig0035450:+11651 None:intergenic
GAAAGTGCATTAGCTAGTAG+AGG 0.576701 tig0035450:+10947 None:intergenic
CTGATTCACTTGGGATACAA+GGG 0.580891 tig0035450:-11164 Msa1454820:three_prime_UTR
CGAAAATGGCTGTGATGGAG+AGG 0.587353 tig0035450:-12331 Msa1454820:exon
AAAGTGCATTAGCTAGTAGA+GGG 0.595231 tig0035450:+10948 None:intergenic
CAACTTGTGATAGTTGTGCA+AGG 0.606837 tig0035450:-12502 Msa1454820:five_prime_UTR
AAAAGTAAGCTGATTCACTT+GGG 0.609457 tig0035450:-11173 Msa1454820:exon
TCGCTCAAAGCAGGTTGAGG+TGG 0.622674 tig0035450:+11660 None:intergenic
TAGAGGGAAAATACATCAGG+TGG 0.624421 tig0035450:+10964 None:intergenic
AAACTCCATATTGTCACTAG+AGG 0.625841 tig0035450:+10915 None:intergenic
AACTTGTGATAGTTGTGCAA+GGG 0.630990 tig0035450:-12501 Msa1454820:five_prime_UTR
TAGGCCTCACAAAAGCAGGA+AGG 0.675417 tig0035450:+11712 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATCATTTCATATAATTA+GGG - tig0035450:11137-11156 Msa1454820:three_prime_UTR 15.0%
!! TTACAGATTTACATTATTAT+CGG + tig0035450:11207-11226 None:intergenic 15.0%
!!! TTCATACAATTTATTTGTTT+TGG + tig0035450:11745-11764 None:intergenic 15.0%
!!! TTTATTATTTTATCTCATCA+CGG - tig0035450:11767-11786 Msa1454820:intron 15.0%
!! TAAATATAAAATGAAGTCTT+TGG + tig0035450:12333-12352 None:intergenic 15.0%
!!! TTATATTTAAGTTAAGTCTT+TGG - tig0035450:12344-12363 Msa1454820:exon 15.0%
!! TCAATCATTTCATATAATTA+GGG - tig0035450:11137-11156 Msa1454820:three_prime_UTR 15.0%
!! TTACAGATTTACATTATTAT+CGG + tig0035450:11207-11226 None:intergenic 15.0%
!!! TTCATACAATTTATTTGTTT+TGG + tig0035450:11745-11764 None:intergenic 15.0%
!!! TTTATTATTTTATCTCATCA+CGG - tig0035450:11767-11786 Msa1454820:intron 15.0%
!! TAAATATAAAATGAAGTCTT+TGG + tig0035450:12333-12352 None:intergenic 15.0%
!!! TTATATTTAAGTTAAGTCTT+TGG - tig0035450:12344-12363 Msa1454820:exon 15.0%
!! CTCAATCATTTCATATAATT+AGG - tig0035450:11136-11155 Msa1454820:three_prime_UTR 20.0%
!!! TTATTATTTTATCTCATCAC+GGG - tig0035450:11768-11787 Msa1454820:intron 20.0%
!! CTCAATCATTTCATATAATT+AGG - tig0035450:11136-11155 Msa1454820:three_prime_UTR 20.0%
!!! TTATTATTTTATCTCATCAC+GGG - tig0035450:11768-11787 Msa1454820:intron 20.0%
! ATGTGACAAAATTGATGTTT+AGG + tig0035450:10918-10937 None:intergenic 25.0%
!! TTTGTCACATGTAACAATTT+TGG - tig0035450:10928-10947 Msa1454820:three_prime_UTR 25.0%
!! GTCGATTACTGAATATTTTT+GGG + tig0035450:10954-10973 None:intergenic 25.0%
!!! TGTCGATTACTGAATATTTT+TGG + tig0035450:10955-10974 None:intergenic 25.0%
! TATATGAAATGATTGAGAAG+AGG + tig0035450:11133-11152 None:intergenic 25.0%
! ATGATAATCTAACCAAATAG+TGG + tig0035450:11306-11325 None:intergenic 25.0%
! TTTGGTTAGATTATCATGTA+TGG - tig0035450:11309-11328 Msa1454820:intron 25.0%
! TAGACAAACAAGATTCAATT+TGG + tig0035450:11337-11356 None:intergenic 25.0%
!!! AGCATAGATAGTTCATTTTT+TGG - tig0035450:11385-11404 Msa1454820:intron 25.0%
!!! GCATAGATAGTTCATTTTTT+GGG - tig0035450:11386-11405 Msa1454820:intron 25.0%
!! CAAAGAAAATTTAAGACCAA+AGG + tig0035450:11484-11503 None:intergenic 25.0%
! AATCATGAAACAGAATAAAC+TGG + tig0035450:11528-11547 None:intergenic 25.0%
!! TACTATGTGAAATTTTCTGT+AGG - tig0035450:11611-11630 Msa1454820:intron 25.0%
!!! TATTATTTTATCTCATCACG+GGG - tig0035450:11769-11788 Msa1454820:intron 25.0%
! TTATCTCAAGTGATACTAAT+GGG + tig0035450:11955-11974 None:intergenic 25.0%
! TTTATCTCAAGTGATACTAA+TGG + tig0035450:11956-11975 None:intergenic 25.0%
! GAAACCATAAATGTAAATGA+TGG + tig0035450:12109-12128 None:intergenic 25.0%
! CATTTATGGTTTCTTGATAT+TGG - tig0035450:12116-12135 Msa1454820:intron 25.0%
!! CAATCAAATGTTTTGTAACT+AGG + tig0035450:12272-12291 None:intergenic 25.0%
! ATGTGACAAAATTGATGTTT+AGG + tig0035450:10918-10937 None:intergenic 25.0%
!! TTTGTCACATGTAACAATTT+TGG - tig0035450:10928-10947 Msa1454820:three_prime_UTR 25.0%
!! GTCGATTACTGAATATTTTT+GGG + tig0035450:10954-10973 None:intergenic 25.0%
!!! TGTCGATTACTGAATATTTT+TGG + tig0035450:10955-10974 None:intergenic 25.0%
! TATATGAAATGATTGAGAAG+AGG + tig0035450:11133-11152 None:intergenic 25.0%
! ATGATAATCTAACCAAATAG+TGG + tig0035450:11306-11325 None:intergenic 25.0%
! TTTGGTTAGATTATCATGTA+TGG - tig0035450:11309-11328 Msa1454820:intron 25.0%
! TAGACAAACAAGATTCAATT+TGG + tig0035450:11337-11356 None:intergenic 25.0%
!!! AGCATAGATAGTTCATTTTT+TGG - tig0035450:11385-11404 Msa1454820:intron 25.0%
!!! GCATAGATAGTTCATTTTTT+GGG - tig0035450:11386-11405 Msa1454820:intron 25.0%
!! CAAAGAAAATTTAAGACCAA+AGG + tig0035450:11484-11503 None:intergenic 25.0%
! AATCATGAAACAGAATAAAC+TGG + tig0035450:11528-11547 None:intergenic 25.0%
!! TACTATGTGAAATTTTCTGT+AGG - tig0035450:11611-11630 Msa1454820:intron 25.0%
!!! TATTATTTTATCTCATCACG+GGG - tig0035450:11769-11788 Msa1454820:intron 25.0%
! TTATCTCAAGTGATACTAAT+GGG + tig0035450:11955-11974 None:intergenic 25.0%
! TTTATCTCAAGTGATACTAA+TGG + tig0035450:11956-11975 None:intergenic 25.0%
! GAAACCATAAATGTAAATGA+TGG + tig0035450:12109-12128 None:intergenic 25.0%
! CATTTATGGTTTCTTGATAT+TGG - tig0035450:12116-12135 Msa1454820:intron 25.0%
!! CAATCAAATGTTTTGTAACT+AGG + tig0035450:12272-12291 None:intergenic 25.0%
ATCGAAATCGAAATCGAAAA+TGG - tig0035450:11007-11026 Msa1454820:three_prime_UTR 30.0%
! TTTTTGTTCCAGCCTTAATT+TGG - tig0035450:11280-11299 Msa1454820:intron 30.0%
! ATCTTGTTTGTCTAAGATTG+AGG - tig0035450:11343-11362 Msa1454820:intron 30.0%
!!! TTCATTTTTTGGGTTACTAC+TGG - tig0035450:11396-11415 Msa1454820:intron 30.0%
ATAGTAAGTGAATAGCATGT+TGG + tig0035450:11597-11616 None:intergenic 30.0%
AATGGGAAAAACAGAATGTA+GGG + tig0035450:11938-11957 None:intergenic 30.0%
TAATGGGAAAAACAGAATGT+AGG + tig0035450:11939-11958 None:intergenic 30.0%
TGTGAGCACTTGAAAATATT+GGG + tig0035450:11982-12001 None:intergenic 30.0%
TTGTGAGCACTTGAAAATAT+TGG + tig0035450:11983-12002 None:intergenic 30.0%
GAGACCATCATTTACATTTA+TGG - tig0035450:12102-12121 Msa1454820:intron 30.0%
! AAAAAGTAAGCTGATTCACT+TGG - tig0035450:12178-12197 Msa1454820:intron 30.0%
! AAAAGTAAGCTGATTCACTT+GGG - tig0035450:12179-12198 Msa1454820:intron 30.0%
AACTGATAGATTGTCACAAA+TGG + tig0035450:12300-12319 None:intergenic 30.0%
ATGAAGTCTTTGGATTAATC+TGG + tig0035450:12323-12342 None:intergenic 30.0%
ATCGAAATCGAAATCGAAAA+TGG - tig0035450:11007-11026 Msa1454820:three_prime_UTR 30.0%
! TTTTTGTTCCAGCCTTAATT+TGG - tig0035450:11280-11299 Msa1454820:intron 30.0%
! ATCTTGTTTGTCTAAGATTG+AGG - tig0035450:11343-11362 Msa1454820:intron 30.0%
!!! TTCATTTTTTGGGTTACTAC+TGG - tig0035450:11396-11415 Msa1454820:intron 30.0%
ATAGTAAGTGAATAGCATGT+TGG + tig0035450:11597-11616 None:intergenic 30.0%
AATGGGAAAAACAGAATGTA+GGG + tig0035450:11938-11957 None:intergenic 30.0%
TAATGGGAAAAACAGAATGT+AGG + tig0035450:11939-11958 None:intergenic 30.0%
TGTGAGCACTTGAAAATATT+GGG + tig0035450:11982-12001 None:intergenic 30.0%
TTGTGAGCACTTGAAAATAT+TGG + tig0035450:11983-12002 None:intergenic 30.0%
GAGACCATCATTTACATTTA+TGG - tig0035450:12102-12121 Msa1454820:intron 30.0%
! AAAAAGTAAGCTGATTCACT+TGG - tig0035450:12178-12197 Msa1454820:intron 30.0%
! AAAAGTAAGCTGATTCACTT+GGG - tig0035450:12179-12198 Msa1454820:intron 30.0%
AACTGATAGATTGTCACAAA+TGG + tig0035450:12300-12319 None:intergenic 30.0%
ATGAAGTCTTTGGATTAATC+TGG + tig0035450:12323-12342 None:intergenic 30.0%
AACTTGTGATAGTTGTGCAA+GGG - tig0035450:10851-10870 Msa1454820:three_prime_UTR 35.0%
!! AAGGGACATTTTGGGAAATT+TGG - tig0035450:10869-10888 Msa1454820:three_prime_UTR 35.0%
!! AGGGACATTTTGGGAAATTT+GGG - tig0035450:10870-10889 Msa1454820:three_prime_UTR 35.0%
GATTGAGAAGAGGAAGATTA+GGG + tig0035450:11123-11142 None:intergenic 35.0%
TGATTGAGAAGAGGAAGATT+AGG + tig0035450:11124-11143 None:intergenic 35.0%
ACCAAATAGTGGCCAAATTA+AGG + tig0035450:11295-11314 None:intergenic 35.0%
! TTGTCTAAGATTGAGGTAGA+TGG - tig0035450:11350-11369 Msa1454820:intron 35.0%
TAAGATTGAGGTAGATGGAA+TGG - tig0035450:11355-11374 Msa1454820:intron 35.0%
CCAAAGGTAAACATGTTATG+AGG + tig0035450:11468-11487 None:intergenic 35.0%
CCTCATAACATGTTTACCTT+TGG - tig0035450:11465-11484 Msa1454820:intron 35.0%
ATGGGAAAAACAGAATGTAG+GGG + tig0035450:11937-11956 None:intergenic 35.0%
CTATTCTTGGAACTAATGGT+GGG + tig0035450:12028-12047 None:intergenic 35.0%
CAAGCTATTCTTGGAACTAA+TGG + tig0035450:12032-12051 None:intergenic 35.0%
! AAGAATAGCTTGTCTCGAAA+GGG - tig0035450:12040-12059 Msa1454820:intron 35.0%
TAAATGATGGTCTCAATCTG+GGG + tig0035450:12096-12115 None:intergenic 35.0%
GTAAATGATGGTCTCAATCT+GGG + tig0035450:12097-12116 None:intergenic 35.0%
TGTAAATGATGGTCTCAATC+TGG + tig0035450:12098-12117 None:intergenic 35.0%
TTGGGATACAAGGGAATAAT+TGG - tig0035450:12197-12216 Msa1454820:intron 35.0%
! GTACTTTTATTACTCACAGC+TGG + tig0035450:12250-12269 None:intergenic 35.0%
TAGTAGAGGGAAAATACATC+AGG + tig0035450:12394-12413 None:intergenic 35.0%
AAAGTGCATTAGCTAGTAGA+GGG + tig0035450:12407-12426 None:intergenic 35.0%
TTGTTCCTCTAGTGACAATA+TGG - tig0035450:12432-12451 Msa1454820:five_prime_UTR 35.0%
AAACTCCATATTGTCACTAG+AGG + tig0035450:12440-12459 None:intergenic 35.0%
AACTTGTGATAGTTGTGCAA+GGG - tig0035450:10851-10870 Msa1454820:three_prime_UTR 35.0%
!! AAGGGACATTTTGGGAAATT+TGG - tig0035450:10869-10888 Msa1454820:three_prime_UTR 35.0%
!! AGGGACATTTTGGGAAATTT+GGG - tig0035450:10870-10889 Msa1454820:three_prime_UTR 35.0%
GATTGAGAAGAGGAAGATTA+GGG + tig0035450:11123-11142 None:intergenic 35.0%
TGATTGAGAAGAGGAAGATT+AGG + tig0035450:11124-11143 None:intergenic 35.0%
ACCAAATAGTGGCCAAATTA+AGG + tig0035450:11295-11314 None:intergenic 35.0%
! TTGTCTAAGATTGAGGTAGA+TGG - tig0035450:11350-11369 Msa1454820:intron 35.0%
TAAGATTGAGGTAGATGGAA+TGG - tig0035450:11355-11374 Msa1454820:intron 35.0%
CCAAAGGTAAACATGTTATG+AGG + tig0035450:11468-11487 None:intergenic 35.0%
CCTCATAACATGTTTACCTT+TGG - tig0035450:11465-11484 Msa1454820:intron 35.0%
ATGGGAAAAACAGAATGTAG+GGG + tig0035450:11937-11956 None:intergenic 35.0%
CTATTCTTGGAACTAATGGT+GGG + tig0035450:12028-12047 None:intergenic 35.0%
CAAGCTATTCTTGGAACTAA+TGG + tig0035450:12032-12051 None:intergenic 35.0%
! AAGAATAGCTTGTCTCGAAA+GGG - tig0035450:12040-12059 Msa1454820:intron 35.0%
TAAATGATGGTCTCAATCTG+GGG + tig0035450:12096-12115 None:intergenic 35.0%
GTAAATGATGGTCTCAATCT+GGG + tig0035450:12097-12116 None:intergenic 35.0%
TGTAAATGATGGTCTCAATC+TGG + tig0035450:12098-12117 None:intergenic 35.0%
TTGGGATACAAGGGAATAAT+TGG - tig0035450:12197-12216 Msa1454820:intron 35.0%
! GTACTTTTATTACTCACAGC+TGG + tig0035450:12250-12269 None:intergenic 35.0%
TAGTAGAGGGAAAATACATC+AGG + tig0035450:12394-12413 None:intergenic 35.0%
AAAGTGCATTAGCTAGTAGA+GGG + tig0035450:12407-12426 None:intergenic 35.0%
TTGTTCCTCTAGTGACAATA+TGG - tig0035450:12432-12451 Msa1454820:five_prime_UTR 35.0%
AAACTCCATATTGTCACTAG+AGG + tig0035450:12440-12459 None:intergenic 35.0%
CAACTTGTGATAGTTGTGCA+AGG - tig0035450:10850-10869 Msa1454820:three_prime_UTR 40.0%
! TAGTTGTGCAAGGGACATTT+TGG - tig0035450:10860-10879 Msa1454820:three_prime_UTR 40.0%
! AGTTGTGCAAGGGACATTTT+GGG - tig0035450:10861-10880 Msa1454820:three_prime_UTR 40.0%
!! GGGACATTTTGGGAAATTTG+GGG - tig0035450:10871-10890 Msa1454820:three_prime_UTR 40.0%
!! GGACATTTTGGGAAATTTGG+GGG - tig0035450:10872-10891 Msa1454820:three_prime_UTR 40.0%
!! GACATTTTGGGAAATTTGGG+GGG - tig0035450:10873-10892 Msa1454820:three_prime_UTR 40.0%
CACAGAACAGAACAGAAGAA+AGG - tig0035450:10973-10992 Msa1454820:three_prime_UTR 40.0%
GAAATCGAAAATGGCTGTGA+TGG - tig0035450:11016-11035 Msa1454820:three_prime_UTR 40.0%
AATAGTGGCCAAATTAAGGC+TGG + tig0035450:11291-11310 None:intergenic 40.0%
! GCCTTAATTTGGCCACTATT+TGG - tig0035450:11291-11310 Msa1454820:intron 40.0%
! TTTGACTTAGCTACCTCACT+GGG - tig0035450:11422-11441 Msa1454820:intron 40.0%
TCACAAAAGCAGGAAGGAAA+AGG + tig0035450:11637-11656 None:intergenic 40.0%
!! TTTTCCTTCCTGCTTTTGTG+AGG - tig0035450:11636-11655 Msa1454820:intron 40.0%
TCTGAAAGACCTGTAAGAAG+AGG - tig0035450:11837-11856 Msa1454820:intron 40.0%
ACCTGTAAGAAGAGGCTTTA+GGG - tig0035450:11845-11864 Msa1454820:intron 40.0%
CTATAATGCTGAAACTCTGC+TGG + tig0035450:11887-11906 None:intergenic 40.0%
GAAAAACAGAATGTAGGGGA+TGG + tig0035450:11933-11952 None:intergenic 40.0%
GCTATTCTTGGAACTAATGG+TGG + tig0035450:12029-12048 None:intergenic 40.0%
CTTTCGAGACAAGCTATTCT+TGG + tig0035450:12041-12060 None:intergenic 40.0%
! CAAGAATAGCTTGTCTCGAA+AGG - tig0035450:12039-12058 Msa1454820:intron 40.0%
! AGAATAGCTTGTCTCGAAAG+GGG - tig0035450:12041-12060 Msa1454820:intron 40.0%
! TTGGTGTTCTATATCGTTGC+AGG - tig0035450:12135-12154 Msa1454820:intron 40.0%
CTGATTCACTTGGGATACAA+GGG - tig0035450:12188-12207 Msa1454820:intron 40.0%
TAGAGGGAAAATACATCAGG+TGG + tig0035450:12391-12410 None:intergenic 40.0%
! GAAAGTGCATTAGCTAGTAG+AGG + tig0035450:12408-12427 None:intergenic 40.0%
CAACTTGTGATAGTTGTGCA+AGG - tig0035450:10850-10869 Msa1454820:three_prime_UTR 40.0%
! TAGTTGTGCAAGGGACATTT+TGG - tig0035450:10860-10879 Msa1454820:three_prime_UTR 40.0%
! AGTTGTGCAAGGGACATTTT+GGG - tig0035450:10861-10880 Msa1454820:three_prime_UTR 40.0%
!! GGGACATTTTGGGAAATTTG+GGG - tig0035450:10871-10890 Msa1454820:three_prime_UTR 40.0%
!! GGACATTTTGGGAAATTTGG+GGG - tig0035450:10872-10891 Msa1454820:three_prime_UTR 40.0%
!! GACATTTTGGGAAATTTGGG+GGG - tig0035450:10873-10892 Msa1454820:three_prime_UTR 40.0%
CACAGAACAGAACAGAAGAA+AGG - tig0035450:10973-10992 Msa1454820:three_prime_UTR 40.0%
GAAATCGAAAATGGCTGTGA+TGG - tig0035450:11016-11035 Msa1454820:three_prime_UTR 40.0%
AATAGTGGCCAAATTAAGGC+TGG + tig0035450:11291-11310 None:intergenic 40.0%
! GCCTTAATTTGGCCACTATT+TGG - tig0035450:11291-11310 Msa1454820:intron 40.0%
! TTTGACTTAGCTACCTCACT+GGG - tig0035450:11422-11441 Msa1454820:intron 40.0%
TCACAAAAGCAGGAAGGAAA+AGG + tig0035450:11637-11656 None:intergenic 40.0%
!! TTTTCCTTCCTGCTTTTGTG+AGG - tig0035450:11636-11655 Msa1454820:intron 40.0%
TCTGAAAGACCTGTAAGAAG+AGG - tig0035450:11837-11856 Msa1454820:intron 40.0%
ACCTGTAAGAAGAGGCTTTA+GGG - tig0035450:11845-11864 Msa1454820:intron 40.0%
CTATAATGCTGAAACTCTGC+TGG + tig0035450:11887-11906 None:intergenic 40.0%
GAAAAACAGAATGTAGGGGA+TGG + tig0035450:11933-11952 None:intergenic 40.0%
GCTATTCTTGGAACTAATGG+TGG + tig0035450:12029-12048 None:intergenic 40.0%
CTTTCGAGACAAGCTATTCT+TGG + tig0035450:12041-12060 None:intergenic 40.0%
! CAAGAATAGCTTGTCTCGAA+AGG - tig0035450:12039-12058 Msa1454820:intron 40.0%
! AGAATAGCTTGTCTCGAAAG+GGG - tig0035450:12041-12060 Msa1454820:intron 40.0%
! TTGGTGTTCTATATCGTTGC+AGG - tig0035450:12135-12154 Msa1454820:intron 40.0%
CTGATTCACTTGGGATACAA+GGG - tig0035450:12188-12207 Msa1454820:intron 40.0%
TAGAGGGAAAATACATCAGG+TGG + tig0035450:12391-12410 None:intergenic 40.0%
! GAAAGTGCATTAGCTAGTAG+AGG + tig0035450:12408-12427 None:intergenic 40.0%
! CTTTGACTTAGCTACCTCAC+TGG - tig0035450:11421-11440 Msa1454820:intron 45.0%
GTTCGATATGTGTCCAGCAT+AGG + tig0035450:11662-11681 None:intergenic 45.0%
AAACTCACATCGCTCAAAGC+AGG + tig0035450:11704-11723 None:intergenic 45.0%
GACCTGTAAGAAGAGGCTTT+AGG - tig0035450:11844-11863 Msa1454820:intron 45.0%
! CCTGTAAGAAGAGGCTTTAG+GGG - tig0035450:11846-11865 Msa1454820:intron 45.0%
GTGTTCTATATCGTTGCAGG+TGG - tig0035450:12138-12157 Msa1454820:intron 45.0%
! GCTGATTCACTTGGGATACA+AGG - tig0035450:12187-12206 Msa1454820:intron 45.0%
! CTTTGACTTAGCTACCTCAC+TGG - tig0035450:11421-11440 Msa1454820:intron 45.0%
GTTCGATATGTGTCCAGCAT+AGG + tig0035450:11662-11681 None:intergenic 45.0%
AAACTCACATCGCTCAAAGC+AGG + tig0035450:11704-11723 None:intergenic 45.0%
GACCTGTAAGAAGAGGCTTT+AGG - tig0035450:11844-11863 Msa1454820:intron 45.0%
! CCTGTAAGAAGAGGCTTTAG+GGG - tig0035450:11846-11865 Msa1454820:intron 45.0%
GTGTTCTATATCGTTGCAGG+TGG - tig0035450:12138-12157 Msa1454820:intron 45.0%
! GCTGATTCACTTGGGATACA+AGG - tig0035450:12187-12206 Msa1454820:intron 45.0%
! CGAAAATGGCTGTGATGGAG+AGG - tig0035450:11021-11040 Msa1454820:three_prime_UTR 50.0%
AGACAAGAAGCAGCGACAAC+TGG + tig0035450:11071-11090 None:intergenic 50.0%
!! TCTTGTCTCATCGCTGGCTT+TGG - tig0035450:11083-11102 Msa1454820:three_prime_UTR 50.0%
TTAGCTACCTCACTGGGTGT+TGG - tig0035450:11428-11447 Msa1454820:intron 50.0%
TAGCTACCTCACTGGGTGTT+GGG - tig0035450:11429-11448 Msa1454820:intron 50.0%
TAGGCCTCACAAAAGCAGGA+AGG + tig0035450:11643-11662 None:intergenic 50.0%
AGCATAGGCCTCACAAAAGC+AGG + tig0035450:11647-11666 None:intergenic 50.0%
! TGCTTTTGTGAGGCCTATGC+TGG - tig0035450:11646-11665 Msa1454820:intron 50.0%
ACATCGCTCAAAGCAGGTTG+AGG + tig0035450:11698-11717 None:intergenic 50.0%
CCCCTAAAGCCTCTTCTTAC+AGG + tig0035450:11849-11868 None:intergenic 50.0%
GTCTCAATCTGGGGAGAGTA+GGG + tig0035450:12087-12106 None:intergenic 50.0%
ATATCGTTGCAGGTGGTTGC+AGG - tig0035450:12145-12164 Msa1454820:intron 50.0%
! CGAAAATGGCTGTGATGGAG+AGG - tig0035450:11021-11040 Msa1454820:three_prime_UTR 50.0%
AGACAAGAAGCAGCGACAAC+TGG + tig0035450:11071-11090 None:intergenic 50.0%
!! TCTTGTCTCATCGCTGGCTT+TGG - tig0035450:11083-11102 Msa1454820:three_prime_UTR 50.0%
TTAGCTACCTCACTGGGTGT+TGG - tig0035450:11428-11447 Msa1454820:intron 50.0%
TAGCTACCTCACTGGGTGTT+GGG - tig0035450:11429-11448 Msa1454820:intron 50.0%
TAGGCCTCACAAAAGCAGGA+AGG + tig0035450:11643-11662 None:intergenic 50.0%
AGCATAGGCCTCACAAAAGC+AGG + tig0035450:11647-11666 None:intergenic 50.0%
! TGCTTTTGTGAGGCCTATGC+TGG - tig0035450:11646-11665 Msa1454820:intron 50.0%
ACATCGCTCAAAGCAGGTTG+AGG + tig0035450:11698-11717 None:intergenic 50.0%
CCCCTAAAGCCTCTTCTTAC+AGG + tig0035450:11849-11868 None:intergenic 50.0%
GTCTCAATCTGGGGAGAGTA+GGG + tig0035450:12087-12106 None:intergenic 50.0%
ATATCGTTGCAGGTGGTTGC+AGG - tig0035450:12145-12164 Msa1454820:intron 50.0%
TTGGGGGGCTGTGAGCAATA+CGG - tig0035450:10888-10907 Msa1454820:three_prime_UTR 55.0%
GCTGCTTCTTGTCTCATCGC+TGG - tig0035450:11077-11096 Msa1454820:three_prime_UTR 55.0%
!! TACCTCACTGGGTGTTGGGA+AGG - tig0035450:11433-11452 Msa1454820:intron 55.0%
! AGCAGGTTGAGGTGGTTGCT+CGG + tig0035450:11687-11706 None:intergenic 55.0%
! TCGCTCAAAGCAGGTTGAGG+TGG + tig0035450:11695-11714 None:intergenic 55.0%
GGTCTCAATCTGGGGAGAGT+AGG + tig0035450:12088-12107 None:intergenic 55.0%
CAGGTGGTTGCAGGTATGAC+TGG - tig0035450:12154-12173 Msa1454820:intron 55.0%
TTGGGGGGCTGTGAGCAATA+CGG - tig0035450:10888-10907 Msa1454820:three_prime_UTR 55.0%
GCTGCTTCTTGTCTCATCGC+TGG - tig0035450:11077-11096 Msa1454820:three_prime_UTR 55.0%
!! TACCTCACTGGGTGTTGGGA+AGG - tig0035450:11433-11452 Msa1454820:intron 55.0%
! AGCAGGTTGAGGTGGTTGCT+CGG + tig0035450:11687-11706 None:intergenic 55.0%
! TCGCTCAAAGCAGGTTGAGG+TGG + tig0035450:11695-11714 None:intergenic 55.0%
GGTCTCAATCTGGGGAGAGT+AGG + tig0035450:12088-12107 None:intergenic 55.0%
CAGGTGGTTGCAGGTATGAC+TGG - tig0035450:12154-12173 Msa1454820:intron 55.0%
TGGGGGGCTGTGAGCAATAC+GGG - tig0035450:10889-10908 Msa1454820:three_prime_UTR 60.0%
CACCTTCCCAACACCCAGTG+AGG + tig0035450:11438-11457 None:intergenic 60.0%
TGGGGGGCTGTGAGCAATAC+GGG - tig0035450:10889-10908 Msa1454820:three_prime_UTR 60.0%
CACCTTCCCAACACCCAGTG+AGG + tig0035450:11438-11457 None:intergenic 60.0%
GGGGGGCTGTGAGCAATACG+GGG - tig0035450:10890-10909 Msa1454820:three_prime_UTR 65.0%
GGGGGCTGTGAGCAATACGG+GGG - tig0035450:10891-10910 Msa1454820:three_prime_UTR 65.0%
GGGGGGCTGTGAGCAATACG+GGG - tig0035450:10890-10909 Msa1454820:three_prime_UTR 65.0%
GGGGGCTGTGAGCAATACGG+GGG - tig0035450:10891-10910 Msa1454820:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
tig0035450 gene 10811 12563 10811 ID=Msa1454820;Name=Msa1454820
tig0035450 mRNA 10811 12563 10811 ID=Msa1454820-mRNA-1;Parent=Msa1454820;Name=Msa1454820-mRNA-1;_AED=0.01;_eAED=0.01;_QI=215|1|1|1|1|1|3|377|63
tig0035450 exon 10811 11217 10811 ID=Msa1454820-mRNA-1:exon:19949;Parent=Msa1454820-mRNA-1
tig0035450 exon 11659 11741 11659 ID=Msa1454820-mRNA-1:exon:19948;Parent=Msa1454820-mRNA-1
tig0035450 exon 12270 12563 12270 ID=Msa1454820-mRNA-1:exon:19947;Parent=Msa1454820-mRNA-1
tig0035450 five_prime_UTR 12349 12563 12349 ID=Msa1454820-mRNA-1:five_prime_utr;Parent=Msa1454820-mRNA-1
tig0035450 CDS 12270 12348 12270 ID=Msa1454820-mRNA-1:cds;Parent=Msa1454820-mRNA-1
tig0035450 CDS 11659 11741 11659 ID=Msa1454820-mRNA-1:cds;Parent=Msa1454820-mRNA-1
tig0035450 CDS 11188 11217 11188 ID=Msa1454820-mRNA-1:cds;Parent=Msa1454820-mRNA-1
tig0035450 three_prime_UTR 10811 11187 10811 ID=Msa1454820-mRNA-1:three_prime_utr;Parent=Msa1454820-mRNA-1
Gene Sequence

>Msa1454820

ATGGCTGTGATGGAGAGGCTAAGAATGTTCGTTGCTCAAGAACCAGTTGTCGCTGCTTCTTGTCTCATCGCTGGCTTTGGCCTTTTCCTTCCTGCTTTTGTGAGGCCTATGCTGGACACATATCGAACAGCCGAGCAACCACCTCAACCTGCTTTGAGCGATGTGGTTGCAGGTATGACTGGTAAAAAGTAA
Protein sequence

>Msa1454820

MAVMERLRMFVAQEPVVAASCLIAGFGLFLPAFVRPMLDTYRTAEQPPQPALSDVVAGMTGKK*