AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1463420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1463420 MtrunA17_Chr1g0207631 99.061 213 2 0 1 213 1 213 5.76e-156 430
Msa1463420 MtrunA17_Chr8g0372661 42.675 157 88 2 25 180 42 197 2.65e-39 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1463420 AT2G18990.1 77.885 208 45 1 6 213 5 211 1.41e-119 338
Msa1463420 AT3G25580.1 77.404 208 45 2 6 213 5 210 1.38e-118 336
Msa1463420 AT3G25580.2 77.073 205 45 2 9 213 3 205 1.30e-116 330
Msa1463420 AT5G66410.1 36.585 205 123 4 7 209 28 227 1.09e-38 133
Msa1463420 AT3G50960.2 42.000 150 85 2 23 171 40 188 4.08e-37 129
Msa1463420 AT3G50960.1 42.000 150 85 2 23 171 40 188 4.08e-37 129

Find 66 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACAAGCATTTAAGTATTT+TGG 0.171906 tig0043289:-2024 Msa1463420:CDS
GTTGCAGCCTCCTTTCTCTT+AGG 0.207376 tig0043289:+2688 None:intergenic
TATATGTTGTGTGTTGAATT+TGG 0.230504 tig0043289:-1180 None:intergenic
TATCAATTGCCAGTCTTGTT+TGG 0.266888 tig0043289:-1386 Msa1463420:three_prime_UTR
GCAAAGGACAAACACATTAA+AGG 0.268507 tig0043289:+1289 Msa1463420:intergenic
TACTGCATAAAAGTAATTCT+TGG 0.286458 tig0043289:+1253 Msa1463420:intergenic
TGACTTTAGCACCGAGGTTT+TGG 0.292143 tig0043289:-1711 Msa1463420:CDS
GTCATTTCTTCCGCGAAAAT+TGG 0.299383 tig0043289:-2545 Msa1463420:CDS
GTTTGAAGGTTCCTTTCGTT+TGG 0.314749 tig0043289:+2912 Msa1463420:intergenic
CGAAATTGTGCGAACCTTGC+AGG 0.328390 tig0043289:+2521 Msa1463420:intergenic
TTGCAGCCTCCTTTCTCTTA+GGG 0.338559 tig0043289:+2689 Msa1463420:intergenic
ATCTGAGCTCTCGTCTTTCC+AGG 0.348336 tig0043289:+1521 Msa1463420:intergenic
GCTCTGCCGAACACTTCTTT+TGG 0.354451 tig0043289:+1610 Msa1463420:intergenic
CTTATAGTGATTAGAATCAT+TGG 0.355260 tig0043289:+1345 Msa1463420:intergenic
TTTGAAGGTTCCTTTCGTTT+GGG 0.386441 tig0043289:+2913 Msa1463420:intergenic
TCAATACACCGTGCAAGGTC+CGG 0.394040 tig0043289:-1641 Msa1463420:CDS
GCTGCAACAAATGAAGAAAA+TGG 0.402598 tig0043289:-2673 Msa1463420:CDS
CTTGATTCTCGATCACCTCT+TGG 0.414878 tig0043289:+2796 Msa1463420:intergenic
CAATACACCGTGCAAGGTCC+GGG 0.431500 tig0043289:-1640 Msa1463420:CDS
ATTAATACGATGCCGTTTGA+AGG 0.465904 tig0043289:+2898 Msa1463420:intergenic
AGAATCATTGGTACAATCAT+CGG 0.469978 tig0043289:+1357 Msa1463420:intergenic
AACATTTGCTTCATGACTAC+AGG 0.477196 tig0043289:+1222 Msa1463420:intergenic
TTTGCGCCCCGGACCTTGCA+CGG 0.490890 tig0043289:+1633 Msa1463420:intergenic
GTCTTTCTCTGACGGAATCT+CGG 0.495284 tig0043289:+2605 Msa1463420:intergenic
TTCTTTATGAGAGCGATAGT+TGG 0.499285 tig0043289:+1915 Msa1463420:intergenic
GAATCATTGGTACAATCATC+GGG 0.502305 tig0043289:+1358 Msa1463420:intergenic
CGCAAACCAAAAGAAGTGTT+CGG 0.506657 tig0043289:-1616 Msa1463420:CDS
CGGTGTAATCTCCGTGTCCG+AGG 0.513907 tig0043289:+2625 Msa1463420:intergenic
ATCTTGAAGCCCTAAGAGAA+AGG 0.527404 tig0043289:-2698 Msa1463420:CDS
CGAAGCAGCATATTGAGACT+CGG 0.534134 tig0043289:-2001 Msa1463420:CDS
CGGGATAATCCAAACAAGAC+TGG 0.546222 tig0043289:+1377 Msa1463420:intergenic
GACGGTGGCGCAAGCAGTCG+AGG 0.556072 tig0043289:-2769 Msa1463420:CDS
TGGTATTAGCACATTTGCAA+AGG 0.556981 tig0043289:+1273 Msa1463420:intergenic
CCGCGAAAATTGGCCCTGCA+AGG 0.557879 tig0043289:-2535 Msa1463420:intron
TACCAAAGTGGATGATTATG+TGG 0.558275 tig0043289:-1892 Msa1463420:intron
AAACGAAAGGAACCTTCAAA+CGG 0.561017 tig0043289:-2910 Msa1463420:five_prime_UTR
GAAATTGTGCGAACCTTGCA+GGG 0.562815 tig0043289:+2522 Msa1463420:intergenic
GAGAAGAAGTCTTTCTCTGA+CGG 0.577836 tig0043289:+2597 Msa1463420:intergenic
AATCATCAATACACCGTGCA+AGG 0.579819 tig0043289:-1646 Msa1463420:CDS
CGTTGGATCTCCCTCGGACA+CGG 0.582223 tig0043289:-2636 Msa1463420:CDS
TACCACATAATCATCCACTT+TGG 0.582640 tig0043289:+1890 Msa1463420:intergenic
CGTAGCCGTTGGATCTCCCT+CGG 0.582964 tig0043289:-2642 Msa1463420:CDS
TGGCGGAGAAGCGTAGCCGT+TGG 0.601854 tig0043289:-2653 Msa1463420:CDS
CAATCTTTCTTCCAAAACCT+CGG 0.602383 tig0043289:+1700 Msa1463420:intergenic
AATTTAGAAAGACACACAAA+CGG 0.604177 tig0043289:+1472 Msa1463420:intergenic
ACGATCACTTGCTTTAACGA+CGG 0.607682 tig0043289:+2575 Msa1463420:intergenic
CGTCATCGTCGAGACGCTCA+AGG 0.607794 tig0043289:+2721 Msa1463420:intergenic
GGTGTAATCTCCGTGTCCGA+GGG 0.608291 tig0043289:+2626 Msa1463420:intergenic
TACAAGTTACTCAGAATCTG+AGG 0.610014 tig0043289:+1574 Msa1463420:intergenic
GAACTCAAAGTGCAGAGCGA+GGG 0.616104 tig0043289:-2867 Msa1463420:five_prime_UTR
GAGCTCTCGTCTTTCCAGGA+GGG 0.622232 tig0043289:+1525 Msa1463420:intergenic
TGAGCTCTCGTCTTTCCAGG+AGG 0.628602 tig0043289:+1524 Msa1463420:intergenic
TGAAATCTATCCCAAACGAA+AGG 0.631935 tig0043289:-2923 Msa1463420:five_prime_UTR
CGTGTCCGAGGGAGATCCAA+CGG 0.633581 tig0043289:+2637 Msa1463420:intergenic
TGAACTCAAAGTGCAGAGCG+AGG 0.639978 tig0043289:-2868 Msa1463420:five_prime_UTR
TCTCATAAAGAATACCAAAG+TGG 0.646699 tig0043289:-1904 Msa1463420:CDS
AATACACCGTGCAAGGTCCG+GGG 0.655774 tig0043289:-1639 Msa1463420:CDS
GATCGAGAATCAAGTACTGA+CGG 0.657827 tig0043289:-2787 Msa1463420:CDS
GTCATCGTCGAGACGCTCAA+GGG 0.663012 tig0043289:+2722 Msa1463420:intergenic
TTGAAGCCCTAAGAGAAAGG+AGG 0.672465 tig0043289:-2695 Msa1463420:CDS
TACTGATGACTTTAGCACCG+AGG 0.705317 tig0043289:-1717 Msa1463420:CDS
GCAACAAATGAAGAAAATGG+CGG 0.709368 tig0043289:-2670 Msa1463420:CDS
AAGTGTTCGGCAGAGCACAA+CGG 0.717985 tig0043289:-1603 Msa1463420:CDS
GGACAAAGGAAAGATCCAAG+AGG 0.724397 tig0043289:-2811 Msa1463420:CDS
AAACTTACAAGACACACGAG+TGG 0.733578 tig0043289:+1437 Msa1463420:intergenic
CGAGAATCAAGTACTGACGG+TGG 0.737424 tig0043289:-2784 Msa1463420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAACTTTTTAATTTAATAAA+AGG - tig0043289:1197-1216 Msa1463420:three_prime_UTR 10.0%
!! GAACTTTATAAAAATATTAT+TGG - tig0043289:1933-1952 Msa1463420:CDS 10.0%
!!! GAACTTTTTAATTTAATAAA+AGG - tig0043289:1197-1216 Msa1463420:three_prime_UTR 10.0%
!! GAACTTTATAAAAATATTAT+TGG - tig0043289:1933-1952 Msa1463420:CDS 10.0%
!! CTCTCAAAAAATAAAATAAA+AGG - tig0043289:1229-1248 Msa1463420:three_prime_UTR 15.0%
!! AAAAAATAAAATAAAAGGAG+AGG - tig0043289:1234-1253 Msa1463420:three_prime_UTR 15.0%
!!! AAGAATTTTAATTTAAACGT+AGG - tig0043289:1911-1930 Msa1463420:CDS 15.0%
!! CTCTCAAAAAATAAAATAAA+AGG - tig0043289:1229-1248 Msa1463420:three_prime_UTR 15.0%
!! AAAAAATAAAATAAAAGGAG+AGG - tig0043289:1234-1253 Msa1463420:three_prime_UTR 15.0%
!!! AAGAATTTTAATTTAAACGT+AGG - tig0043289:1911-1930 Msa1463420:CDS 15.0%
!! ACATGTGATTACATAATTAT+CGG - tig0043289:1797-1816 Msa1463420:intron 20.0%
!! TAGAATTATCTGTTTATCAT+TGG - tig0043289:2028-2047 Msa1463420:CDS 20.0%
!! TATTGACATGAATTCTATAT+TGG - tig0043289:2374-2393 Msa1463420:intron 20.0%
!!! TGTCTAAATTTTGTTTTTGT+TGG - tig0043289:2707-2726 Msa1463420:CDS 20.0%
!!! CTTTCTAAATTTAGTTTTGT+TGG - tig0043289:2742-2761 Msa1463420:CDS 20.0%
!! ACATGTGATTACATAATTAT+CGG - tig0043289:1797-1816 Msa1463420:intron 20.0%
!! TAGAATTATCTGTTTATCAT+TGG - tig0043289:2028-2047 Msa1463420:CDS 20.0%
!! TATTGACATGAATTCTATAT+TGG - tig0043289:2374-2393 Msa1463420:intron 20.0%
!!! TGTCTAAATTTTGTTTTTGT+TGG - tig0043289:2707-2726 Msa1463420:CDS 20.0%
!!! CTTTCTAAATTTAGTTTTGT+TGG - tig0043289:2742-2761 Msa1463420:CDS 20.0%
! AATTATGTAATCACATGTGT+AGG + tig0043289:1795-1814 Msa1463420:intergenic 25.0%
! AATTATCTGTTTATCATTGG+AGG - tig0043289:2031-2050 Msa1463420:CDS 25.0%
! ATTATCTGTTTATCATTGGA+GGG - tig0043289:2032-2051 Msa1463420:CDS 25.0%
! ATATAAAAACTGTAAGTCTG+CGG + tig0043289:2102-2121 Msa1463420:intergenic 25.0%
! TACTAACATCAAGATGATAA+TGG - tig0043289:2427-2446 Msa1463420:intron 25.0%
!!! ATGATAATGGTATGTGTTTT+AGG - tig0043289:2440-2459 Msa1463420:intron 25.0%
! AATTTAGAAAGACACACAAA+CGG + tig0043289:2733-2752 Msa1463420:intergenic 25.0%
! CTTATAGTGATTAGAATCAT+TGG + tig0043289:2860-2879 Msa1463420:intergenic 25.0%
!!! TAATACTAAGAGTTTTGAGA+TGG - tig0043289:2880-2899 Msa1463420:five_prime_UTR 25.0%
! TACTGCATAAAAGTAATTCT+TGG + tig0043289:2952-2971 Msa1463420:intergenic 25.0%
! AATTATGTAATCACATGTGT+AGG + tig0043289:1795-1814 Msa1463420:intergenic 25.0%
! AATTATCTGTTTATCATTGG+AGG - tig0043289:2031-2050 Msa1463420:CDS 25.0%
! ATTATCTGTTTATCATTGGA+GGG - tig0043289:2032-2051 Msa1463420:CDS 25.0%
! ATATAAAAACTGTAAGTCTG+CGG + tig0043289:2102-2121 Msa1463420:intergenic 25.0%
! TACTAACATCAAGATGATAA+TGG - tig0043289:2427-2446 Msa1463420:intron 25.0%
!!! ATGATAATGGTATGTGTTTT+AGG - tig0043289:2440-2459 Msa1463420:intron 25.0%
! AATTTAGAAAGACACACAAA+CGG + tig0043289:2733-2752 Msa1463420:intergenic 25.0%
! CTTATAGTGATTAGAATCAT+TGG + tig0043289:2860-2879 Msa1463420:intergenic 25.0%
!!! TAATACTAAGAGTTTTGAGA+TGG - tig0043289:2880-2899 Msa1463420:five_prime_UTR 25.0%
! TACTGCATAAAAGTAATTCT+TGG + tig0043289:2952-2971 Msa1463420:intergenic 25.0%
AGATCTAGGAGTGAAAAAAA+TGG - tig0043289:1370-1389 Msa1463420:three_prime_UTR 30.0%
TGATTACATAATTATCGGTG+TGG - tig0043289:1802-1821 Msa1463420:intron 30.0%
CGGAATTCAAATTCAAATTG+TGG + tig0043289:2082-2101 Msa1463420:intergenic 30.0%
! TATTTTCTTTCGCTGAAATG+AGG - tig0043289:2119-2138 Msa1463420:intron 30.0%
! GGACAAGCATTTAAGTATTT+TGG - tig0043289:2178-2197 Msa1463420:intron 30.0%
TCTCATAAAGAATACCAAAG+TGG - tig0043289:2298-2317 Msa1463420:intron 30.0%
ATTATGTGGTATGTGCTTAA+AGG - tig0043289:2324-2343 Msa1463420:intron 30.0%
ACATGAATTCTATATTGGAC+AGG - tig0043289:2379-2398 Msa1463420:intron 30.0%
CATGAATTCTATATTGGACA+GGG - tig0043289:2380-2399 Msa1463420:intron 30.0%
!! ATAATGGTATGTGTTTTAGG+TGG - tig0043289:2443-2462 Msa1463420:intron 30.0%
!! TAATGGTATGTGTTTTAGGT+GGG - tig0043289:2444-2463 Msa1463420:intron 30.0%
! TAAAGCTCAAGTCATCTTTT+TGG - tig0043289:2527-2546 Msa1463420:intron 30.0%
AGAATCATTGGTACAATCAT+CGG + tig0043289:2848-2867 Msa1463420:intergenic 30.0%
AGATCTAGGAGTGAAAAAAA+TGG - tig0043289:1370-1389 Msa1463420:three_prime_UTR 30.0%
TGATTACATAATTATCGGTG+TGG - tig0043289:1802-1821 Msa1463420:intron 30.0%
CGGAATTCAAATTCAAATTG+TGG + tig0043289:2082-2101 Msa1463420:intergenic 30.0%
! TATTTTCTTTCGCTGAAATG+AGG - tig0043289:2119-2138 Msa1463420:intron 30.0%
! GGACAAGCATTTAAGTATTT+TGG - tig0043289:2178-2197 Msa1463420:intron 30.0%
TCTCATAAAGAATACCAAAG+TGG - tig0043289:2298-2317 Msa1463420:intron 30.0%
ATTATGTGGTATGTGCTTAA+AGG - tig0043289:2324-2343 Msa1463420:intron 30.0%
ACATGAATTCTATATTGGAC+AGG - tig0043289:2379-2398 Msa1463420:intron 30.0%
CATGAATTCTATATTGGACA+GGG - tig0043289:2380-2399 Msa1463420:intron 30.0%
!! ATAATGGTATGTGTTTTAGG+TGG - tig0043289:2443-2462 Msa1463420:intron 30.0%
!! TAATGGTATGTGTTTTAGGT+GGG - tig0043289:2444-2463 Msa1463420:intron 30.0%
! TAAAGCTCAAGTCATCTTTT+TGG - tig0043289:2527-2546 Msa1463420:intron 30.0%
AGAATCATTGGTACAATCAT+CGG + tig0043289:2848-2867 Msa1463420:intergenic 30.0%
TGAAATCTATCCCAAACGAA+AGG - tig0043289:1279-1298 Msa1463420:three_prime_UTR 35.0%
TTTGAAGGTTCCTTTCGTTT+GGG + tig0043289:1292-1311 Msa1463420:intergenic 35.0%
AAACGAAAGGAACCTTCAAA+CGG - tig0043289:1292-1311 Msa1463420:three_prime_UTR 35.0%
! ATTAATACGATGCCGTTTGA+AGG + tig0043289:1307-1326 Msa1463420:intergenic 35.0%
GGAGTGAAAAAAATGGACAA+AGG - tig0043289:1377-1396 Msa1463420:three_prime_UTR 35.0%
GCTGCAACAAATGAAGAAAA+TGG - tig0043289:1529-1548 Msa1463420:three_prime_UTR 35.0%
GCAACAAATGAAGAAAATGG+CGG - tig0043289:1532-1551 Msa1463420:three_prime_UTR 35.0%
! TGCATTCAGTTTGATTAGCT+TGG - tig0043289:1883-1902 Msa1463420:intron 35.0%
ATATTATTGGCAGTGCATAC+TGG - tig0043289:1946-1965 Msa1463420:CDS 35.0%
TTGAGCTTCTCAGCTAAAAA+TGG + tig0043289:2254-2273 Msa1463420:intergenic 35.0%
TTCTTTATGAGAGCGATAGT+TGG + tig0043289:2290-2309 Msa1463420:intergenic 35.0%
TACCAAAGTGGATGATTATG+TGG - tig0043289:2310-2329 Msa1463420:intron 35.0%
TACCACATAATCATCCACTT+TGG + tig0043289:2315-2334 Msa1463420:intergenic 35.0%
CAATCTTTCTTCCAAAACCT+CGG + tig0043289:2505-2524 Msa1463420:intergenic 35.0%
TACAAGTTACTCAGAATCTG+AGG + tig0043289:2631-2650 Msa1463420:intergenic 35.0%
!! TATCAATTGCCAGTCTTGTT+TGG - tig0043289:2816-2835 Msa1463420:CDS 35.0%
GAATCATTGGTACAATCATC+GGG + tig0043289:2847-2866 Msa1463420:intergenic 35.0%
GCAAAGGACAAACACATTAA+AGG + tig0043289:2916-2935 Msa1463420:intergenic 35.0%
TGGTATTAGCACATTTGCAA+AGG + tig0043289:2932-2951 Msa1463420:intergenic 35.0%
AACATTTGCTTCATGACTAC+AGG + tig0043289:2983-3002 Msa1463420:intergenic 35.0%
TGAAATCTATCCCAAACGAA+AGG - tig0043289:1279-1298 Msa1463420:three_prime_UTR 35.0%
TTTGAAGGTTCCTTTCGTTT+GGG + tig0043289:1292-1311 Msa1463420:intergenic 35.0%
AAACGAAAGGAACCTTCAAA+CGG - tig0043289:1292-1311 Msa1463420:three_prime_UTR 35.0%
! ATTAATACGATGCCGTTTGA+AGG + tig0043289:1307-1326 Msa1463420:intergenic 35.0%
GGAGTGAAAAAAATGGACAA+AGG - tig0043289:1377-1396 Msa1463420:three_prime_UTR 35.0%
GCTGCAACAAATGAAGAAAA+TGG - tig0043289:1529-1548 Msa1463420:three_prime_UTR 35.0%
GCAACAAATGAAGAAAATGG+CGG - tig0043289:1532-1551 Msa1463420:three_prime_UTR 35.0%
! TGCATTCAGTTTGATTAGCT+TGG - tig0043289:1883-1902 Msa1463420:intron 35.0%
ATATTATTGGCAGTGCATAC+TGG - tig0043289:1946-1965 Msa1463420:CDS 35.0%
TTGAGCTTCTCAGCTAAAAA+TGG + tig0043289:2254-2273 Msa1463420:intergenic 35.0%
TTCTTTATGAGAGCGATAGT+TGG + tig0043289:2290-2309 Msa1463420:intergenic 35.0%
TACCAAAGTGGATGATTATG+TGG - tig0043289:2310-2329 Msa1463420:intron 35.0%
TACCACATAATCATCCACTT+TGG + tig0043289:2315-2334 Msa1463420:intergenic 35.0%
CAATCTTTCTTCCAAAACCT+CGG + tig0043289:2505-2524 Msa1463420:intergenic 35.0%
TACAAGTTACTCAGAATCTG+AGG + tig0043289:2631-2650 Msa1463420:intergenic 35.0%
!! TATCAATTGCCAGTCTTGTT+TGG - tig0043289:2816-2835 Msa1463420:CDS 35.0%
GAATCATTGGTACAATCATC+GGG + tig0043289:2847-2866 Msa1463420:intergenic 35.0%
GCAAAGGACAAACACATTAA+AGG + tig0043289:2916-2935 Msa1463420:intergenic 35.0%
TGGTATTAGCACATTTGCAA+AGG + tig0043289:2932-2951 Msa1463420:intergenic 35.0%
AACATTTGCTTCATGACTAC+AGG + tig0043289:2983-3002 Msa1463420:intergenic 35.0%
GTTTGAAGGTTCCTTTCGTT+TGG + tig0043289:1293-1312 Msa1463420:intergenic 40.0%
!! GATCGAGAATCAAGTACTGA+CGG - tig0043289:1415-1434 Msa1463420:three_prime_UTR 40.0%
ATCTTGAAGCCCTAAGAGAA+AGG - tig0043289:1504-1523 Msa1463420:three_prime_UTR 40.0%
GAGAAGAAGTCTTTCTCTGA+CGG + tig0043289:1608-1627 Msa1463420:intergenic 40.0%
ACGATCACTTGCTTTAACGA+CGG + tig0043289:1630-1649 Msa1463420:intergenic 40.0%
GTCATTTCTTCCGCGAAAAT+TGG - tig0043289:1657-1676 Msa1463420:CDS 40.0%
GTAATCACATGTGTAGGTGT+CGG + tig0043289:1789-1808 Msa1463420:intergenic 40.0%
!! TAGGTGGGTTTTGATGAACT+TGG - tig0043289:2459-2478 Msa1463420:intron 40.0%
!!! CGAGGTTTTGGAAGAAAGAT+TGG - tig0043289:2503-2522 Msa1463420:intron 40.0%
! AGCTCAAGTCATCTTTTTGG+AGG - tig0043289:2530-2549 Msa1463420:intron 40.0%
! GCTCAAGTCATCTTTTTGGA+GGG - tig0043289:2531-2550 Msa1463420:intron 40.0%
AATCATCAATACACCGTGCA+AGG - tig0043289:2556-2575 Msa1463420:CDS 40.0%
!! CGCAAACCAAAAGAAGTGTT+CGG - tig0043289:2586-2605 Msa1463420:CDS 40.0%
!! TTTTATTTACGTTGCCCTCC+TGG - tig0043289:2663-2682 Msa1463420:CDS 40.0%
AAACTTACAAGACACACGAG+TGG + tig0043289:2768-2787 Msa1463420:intergenic 40.0%
GTTTGAAGGTTCCTTTCGTT+TGG + tig0043289:1293-1312 Msa1463420:intergenic 40.0%
!! GATCGAGAATCAAGTACTGA+CGG - tig0043289:1415-1434 Msa1463420:three_prime_UTR 40.0%
ATCTTGAAGCCCTAAGAGAA+AGG - tig0043289:1504-1523 Msa1463420:three_prime_UTR 40.0%
GAGAAGAAGTCTTTCTCTGA+CGG + tig0043289:1608-1627 Msa1463420:intergenic 40.0%
ACGATCACTTGCTTTAACGA+CGG + tig0043289:1630-1649 Msa1463420:intergenic 40.0%
GTCATTTCTTCCGCGAAAAT+TGG - tig0043289:1657-1676 Msa1463420:CDS 40.0%
GTAATCACATGTGTAGGTGT+CGG + tig0043289:1789-1808 Msa1463420:intergenic 40.0%
!! TAGGTGGGTTTTGATGAACT+TGG - tig0043289:2459-2478 Msa1463420:intron 40.0%
!!! CGAGGTTTTGGAAGAAAGAT+TGG - tig0043289:2503-2522 Msa1463420:intron 40.0%
! AGCTCAAGTCATCTTTTTGG+AGG - tig0043289:2530-2549 Msa1463420:intron 40.0%
! GCTCAAGTCATCTTTTTGGA+GGG - tig0043289:2531-2550 Msa1463420:intron 40.0%
AATCATCAATACACCGTGCA+AGG - tig0043289:2556-2575 Msa1463420:CDS 40.0%
!! CGCAAACCAAAAGAAGTGTT+CGG - tig0043289:2586-2605 Msa1463420:CDS 40.0%
!! TTTTATTTACGTTGCCCTCC+TGG - tig0043289:2663-2682 Msa1463420:CDS 40.0%
AAACTTACAAGACACACGAG+TGG + tig0043289:2768-2787 Msa1463420:intergenic 40.0%
GGACAAAGGAAAGATCCAAG+AGG - tig0043289:1391-1410 Msa1463420:three_prime_UTR 45.0%
CTTGATTCTCGATCACCTCT+TGG + tig0043289:1409-1428 Msa1463420:intergenic 45.0%
TTGAAGCCCTAAGAGAAAGG+AGG - tig0043289:1507-1526 Msa1463420:three_prime_UTR 45.0%
TTGCAGCCTCCTTTCTCTTA+GGG + tig0043289:1516-1535 Msa1463420:intergenic 45.0%
GTCTTTCTCTGACGGAATCT+CGG + tig0043289:1600-1619 Msa1463420:intergenic 45.0%
GAAATTGTGCGAACCTTGCA+GGG + tig0043289:1683-1702 Msa1463420:intergenic 45.0%
TGTGCTCACAGTTCATTCAC+AGG - tig0043289:2151-2170 Msa1463420:intron 45.0%
CACAGTTCATTCACAGGTTG+TGG - tig0043289:2157-2176 Msa1463420:intron 45.0%
CGAAGCAGCATATTGAGACT+CGG - tig0043289:2201-2220 Msa1463420:intron 45.0%
!! GTGGGTTTTGATGAACTTGG+TGG - tig0043289:2462-2481 Msa1463420:intron 45.0%
TACTGATGACTTTAGCACCG+AGG - tig0043289:2485-2504 Msa1463420:intron 45.0%
!! TGACTTTAGCACCGAGGTTT+TGG - tig0043289:2491-2510 Msa1463420:intron 45.0%
CGGGATAATCCAAACAAGAC+TGG + tig0043289:2828-2847 Msa1463420:intergenic 45.0%
GGACAAAGGAAAGATCCAAG+AGG - tig0043289:1391-1410 Msa1463420:three_prime_UTR 45.0%
CTTGATTCTCGATCACCTCT+TGG + tig0043289:1409-1428 Msa1463420:intergenic 45.0%
TTGAAGCCCTAAGAGAAAGG+AGG - tig0043289:1507-1526 Msa1463420:three_prime_UTR 45.0%
TTGCAGCCTCCTTTCTCTTA+GGG + tig0043289:1516-1535 Msa1463420:intergenic 45.0%
GTCTTTCTCTGACGGAATCT+CGG + tig0043289:1600-1619 Msa1463420:intergenic 45.0%
GAAATTGTGCGAACCTTGCA+GGG + tig0043289:1683-1702 Msa1463420:intergenic 45.0%
TGTGCTCACAGTTCATTCAC+AGG - tig0043289:2151-2170 Msa1463420:intron 45.0%
CACAGTTCATTCACAGGTTG+TGG - tig0043289:2157-2176 Msa1463420:intron 45.0%
CGAAGCAGCATATTGAGACT+CGG - tig0043289:2201-2220 Msa1463420:intron 45.0%
!! GTGGGTTTTGATGAACTTGG+TGG - tig0043289:2462-2481 Msa1463420:intron 45.0%
TACTGATGACTTTAGCACCG+AGG - tig0043289:2485-2504 Msa1463420:intron 45.0%
!! TGACTTTAGCACCGAGGTTT+TGG - tig0043289:2491-2510 Msa1463420:intron 45.0%
CGGGATAATCCAAACAAGAC+TGG + tig0043289:2828-2847 Msa1463420:intergenic 45.0%
TGAACTCAAAGTGCAGAGCG+AGG - tig0043289:1334-1353 Msa1463420:three_prime_UTR 50.0%
GAACTCAAAGTGCAGAGCGA+GGG - tig0043289:1335-1354 Msa1463420:three_prime_UTR 50.0%
GGAGAGAGAGAGAGAGATCT+AGG - tig0043289:1356-1375 Msa1463420:three_prime_UTR 50.0%
!! CGAGAATCAAGTACTGACGG+TGG - tig0043289:1418-1437 Msa1463420:three_prime_UTR 50.0%
GTTGCAGCCTCCTTTCTCTT+AGG + tig0043289:1517-1536 Msa1463420:intergenic 50.0%
CGAAATTGTGCGAACCTTGC+AGG + tig0043289:1684-1703 Msa1463420:intergenic 50.0%
CGGTGTGGATGTGTAGTGTT+AGG - tig0043289:1817-1836 Msa1463420:intron 50.0%
TCAATACACCGTGCAAGGTC+CGG - tig0043289:2561-2580 Msa1463420:CDS 50.0%
!!! ACTTCTTTTGGTTTGCGCCC+CGG + tig0043289:2583-2602 Msa1463420:intergenic 50.0%
! GCTCTGCCGAACACTTCTTT+TGG + tig0043289:2595-2614 Msa1463420:intergenic 50.0%
AAGTGTTCGGCAGAGCACAA+CGG - tig0043289:2599-2618 Msa1463420:CDS 50.0%
ATCTGAGCTCTCGTCTTTCC+AGG + tig0043289:2684-2703 Msa1463420:intergenic 50.0%
TGAACTCAAAGTGCAGAGCG+AGG - tig0043289:1334-1353 Msa1463420:three_prime_UTR 50.0%
GAACTCAAAGTGCAGAGCGA+GGG - tig0043289:1335-1354 Msa1463420:three_prime_UTR 50.0%
GGAGAGAGAGAGAGAGATCT+AGG - tig0043289:1356-1375 Msa1463420:three_prime_UTR 50.0%
!! CGAGAATCAAGTACTGACGG+TGG - tig0043289:1418-1437 Msa1463420:three_prime_UTR 50.0%
GTTGCAGCCTCCTTTCTCTT+AGG + tig0043289:1517-1536 Msa1463420:intergenic 50.0%
CGAAATTGTGCGAACCTTGC+AGG + tig0043289:1684-1703 Msa1463420:intergenic 50.0%
CGGTGTGGATGTGTAGTGTT+AGG - tig0043289:1817-1836 Msa1463420:intron 50.0%
TCAATACACCGTGCAAGGTC+CGG - tig0043289:2561-2580 Msa1463420:CDS 50.0%
!!! ACTTCTTTTGGTTTGCGCCC+CGG + tig0043289:2583-2602 Msa1463420:intergenic 50.0%
! GCTCTGCCGAACACTTCTTT+TGG + tig0043289:2595-2614 Msa1463420:intergenic 50.0%
AAGTGTTCGGCAGAGCACAA+CGG - tig0043289:2599-2618 Msa1463420:CDS 50.0%
ATCTGAGCTCTCGTCTTTCC+AGG + tig0043289:2684-2703 Msa1463420:intergenic 50.0%
GTCATCGTCGAGACGCTCAA+GGG + tig0043289:1483-1502 Msa1463420:intergenic 55.0%
GGTGTAATCTCCGTGTCCGA+GGG + tig0043289:1579-1598 Msa1463420:intergenic 55.0%
! CCTTGCAGGGCCAATTTTCG+CGG + tig0043289:1670-1689 Msa1463420:intergenic 55.0%
CAATACACCGTGCAAGGTCC+GGG - tig0043289:2562-2581 Msa1463420:CDS 55.0%
AATACACCGTGCAAGGTCCG+GGG - tig0043289:2563-2582 Msa1463420:CDS 55.0%
GAGCTCTCGTCTTTCCAGGA+GGG + tig0043289:2680-2699 Msa1463420:intergenic 55.0%
TGAGCTCTCGTCTTTCCAGG+AGG + tig0043289:2681-2700 Msa1463420:intergenic 55.0%
GTCATCGTCGAGACGCTCAA+GGG + tig0043289:1483-1502 Msa1463420:intergenic 55.0%
GGTGTAATCTCCGTGTCCGA+GGG + tig0043289:1579-1598 Msa1463420:intergenic 55.0%
! CCTTGCAGGGCCAATTTTCG+CGG + tig0043289:1670-1689 Msa1463420:intergenic 55.0%
CAATACACCGTGCAAGGTCC+GGG - tig0043289:2562-2581 Msa1463420:CDS 55.0%
AATACACCGTGCAAGGTCCG+GGG - tig0043289:2563-2582 Msa1463420:CDS 55.0%
GAGCTCTCGTCTTTCCAGGA+GGG + tig0043289:2680-2699 Msa1463420:intergenic 55.0%
TGAGCTCTCGTCTTTCCAGG+AGG + tig0043289:2681-2700 Msa1463420:intergenic 55.0%
CGTCATCGTCGAGACGCTCA+AGG + tig0043289:1484-1503 Msa1463420:intergenic 60.0%
CGTAGCCGTTGGATCTCCCT+CGG - tig0043289:1560-1579 Msa1463420:three_prime_UTR 60.0%
CGTGTCCGAGGGAGATCCAA+CGG + tig0043289:1568-1587 Msa1463420:intergenic 60.0%
CGTTGGATCTCCCTCGGACA+CGG - tig0043289:1566-1585 Msa1463420:exon 60.0%
CGGTGTAATCTCCGTGTCCG+AGG + tig0043289:1580-1599 Msa1463420:intergenic 60.0%
CCGCGAAAATTGGCCCTGCA+AGG - tig0043289:1667-1686 Msa1463420:CDS 60.0%
CGTCATCGTCGAGACGCTCA+AGG + tig0043289:1484-1503 Msa1463420:intergenic 60.0%
CGTAGCCGTTGGATCTCCCT+CGG - tig0043289:1560-1579 Msa1463420:three_prime_UTR 60.0%
CGTGTCCGAGGGAGATCCAA+CGG + tig0043289:1568-1587 Msa1463420:intergenic 60.0%
CGTTGGATCTCCCTCGGACA+CGG - tig0043289:1566-1585 Msa1463420:exon 60.0%
CGGTGTAATCTCCGTGTCCG+AGG + tig0043289:1580-1599 Msa1463420:intergenic 60.0%
CCGCGAAAATTGGCCCTGCA+AGG - tig0043289:1667-1686 Msa1463420:CDS 60.0%
! TGGCGGAGAAGCGTAGCCGT+TGG - tig0043289:1549-1568 Msa1463420:three_prime_UTR 65.0%
TTTGCGCCCCGGACCTTGCA+CGG + tig0043289:2572-2591 Msa1463420:intergenic 65.0%
! TGGCGGAGAAGCGTAGCCGT+TGG - tig0043289:1549-1568 Msa1463420:three_prime_UTR 65.0%
TTTGCGCCCCGGACCTTGCA+CGG + tig0043289:2572-2591 Msa1463420:intergenic 65.0%
GACGGTGGCGCAAGCAGTCG+AGG - tig0043289:1433-1452 Msa1463420:three_prime_UTR 70.0%
GACGGTGGCGCAAGCAGTCG+AGG - tig0043289:1433-1452 Msa1463420:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
tig0043289 gene 1182 3042 1182 ID=Msa1463420;Name=Msa1463420
tig0043289 mRNA 1182 3042 1182 ID=Msa1463420-mRNA-1;Parent=Msa1463420;Name=Msa1463420-mRNA-1;_AED=0.01;_eAED=0.01;_QI=207|1|1|1|1|1|3|398|213
tig0043289 exon 1182 1762 1182 ID=Msa1463420-mRNA-1:exon:24045;Parent=Msa1463420-mRNA-1
tig0043289 exon 1893 2051 1893 ID=Msa1463420-mRNA-1:exon:24044;Parent=Msa1463420-mRNA-1
tig0043289 exon 2536 3042 2536 ID=Msa1463420-mRNA-1:exon:24043;Parent=Msa1463420-mRNA-1
tig0043289 five_prime_UTR 2836 3042 2836 ID=Msa1463420-mRNA-1:five_prime_utr;Parent=Msa1463420-mRNA-1
tig0043289 CDS 2536 2835 2536 ID=Msa1463420-mRNA-1:cds;Parent=Msa1463420-mRNA-1
tig0043289 CDS 1893 2051 1893 ID=Msa1463420-mRNA-1:cds;Parent=Msa1463420-mRNA-1
tig0043289 CDS 1580 1762 1580 ID=Msa1463420-mRNA-1:cds;Parent=Msa1463420-mRNA-1
tig0043289 three_prime_UTR 1182 1579 1182 ID=Msa1463420-mRNA-1:three_prime_utr;Parent=Msa1463420-mRNA-1
Gene Sequence

>Msa1463420

ATGGACAAAGGAAAGATCCAAGAGGTGATCGAGAATCAAGTACTGACGGTGGCGCAAGCAGTCGAGGATAAGATCGACGAAGAGATTGCTGCCCTTGAGCGTCTCGACGATGACGATCTTGAAGCCCTAAGAGAAAGGAGGCTGCAACAAATGAAGAAAATGGCGGAGAAGCGTAGCCGTTGGATCTCCCTCGGACACGGAGATTACACCGAGATTCCGTCAGAGAAAGACTTCTTCTCCGTCGTTAAAGCAAGTGATCGTGTTGTTTGTCATTTCTTCCGCGAAAATTGGCCCTGCAAGGTTGTGGACAAGCATTTAAGTATTTTGGCGAAGCAGCATATTGAGACTCGGTTTGTGAAAATAAATGCTGAAAAAAGTCCATTTTTAGCTGAGAAGCTCAAGATCATTGTTCTTCCAACTATCGCTCTCATAAAGAATACCAAAGTGGATGATTATGTGGTGGGTTTTGATGAACTTGGTGGTACTGATGACTTTAGCACCGAGGTTTTGGAAGAAAGATTGGCTAAAGCTCAAGTCATCTTTTTGGAGGGTGAATCATCAATACACCGTGCAAGGTCCGGGGCGCAAACCAAAAGAAGTGTTCGGCAGAGCACAACGGCTGACTCCTCAGATTCTGAGTAA
Protein sequence

>Msa1463420

MDKGKIQEVIENQVLTVAQAVEDKIDEEIAALERLDDDDLEALRERRLQQMKKMAEKRSRWISLGHGDYTEIPSEKDFFSVVKASDRVVCHFFRENWPCKVVDKHLSILAKQHIETRFVKINAEKSPFLAEKLKIIVLPTIALIKNTKVDDYVVGFDELGGTDDFSTEVLEERLAKAQVIFLEGESSIHRARSGAQTKRSVRQSTTADSSDSE*