AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136320 MtrunA17_Chr1g0207631 99.061 213 2 0 1 213 1 213 5.76e-156 430
Msa0136320 MtrunA17_Chr8g0372661 42.675 157 88 2 25 180 42 197 2.65e-39 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136320 AT2G18990.1 77.885 208 45 1 6 213 5 211 1.41e-119 338
Msa0136320 AT3G25580.1 77.404 208 45 2 6 213 5 210 1.38e-118 336
Msa0136320 AT3G25580.2 77.073 205 45 2 9 213 3 205 1.30e-116 330
Msa0136320 AT5G66410.1 36.585 205 123 4 7 209 28 227 1.09e-38 133
Msa0136320 AT3G50960.2 42.000 150 85 2 23 171 40 188 4.08e-37 129
Msa0136320 AT3G50960.1 42.000 150 85 2 23 171 40 188 4.08e-37 129

Find 65 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACAAGCATTTAAGTATTT+TGG 0.171906 1_3:-68159566 Msa0136320:CDS
GTTGCAGCCTCCTTTCTCTT+AGG 0.207376 1_3:+68160231 None:intergenic
TATATGTTGTGTGTTGAATT+TGG 0.230504 1_3:-68158722 None:intergenic
TATCAATTGCCAGTCTTGTT+TGG 0.266888 1_3:-68158928 Msa0136320:three_prime_UTR
GCAAAGGACAAACACATTAA+AGG 0.268507 1_3:+68158831 None:intergenic
TACTGCATAAAAGTAATTCT+TGG 0.286458 1_3:+68158795 None:intergenic
TGACTTTAGCACCGAGGTTT+TGG 0.292143 1_3:-68159253 Msa0136320:CDS
GTCATTTCTTCCGCGAAAAT+TGG 0.299383 1_3:-68160088 Msa0136320:CDS
GTTTGAAGGTTCCTTTCGTT+TGG 0.314749 1_3:+68160455 None:intergenic
CGAAATTGTGCGAACCTTGC+AGG 0.328390 1_3:+68160064 None:intergenic
TTGCAGCCTCCTTTCTCTTA+GGG 0.338559 1_3:+68160232 None:intergenic
ATCTGAGCTCTCGTCTTTCC+AGG 0.348336 1_3:+68159063 None:intergenic
GCTCTGCCGAACACTTCTTT+TGG 0.354451 1_3:+68159152 None:intergenic
CTTATAGTGATTAGAATCAT+TGG 0.355260 1_3:+68158887 None:intergenic
TTTGAAGGTTCCTTTCGTTT+GGG 0.386441 1_3:+68160456 None:intergenic
TCAATACACCGTGCAAGGTC+CGG 0.394040 1_3:-68159183 Msa0136320:CDS
GCTGCAACAAATGAAGAAAA+TGG 0.402598 1_3:-68160216 Msa0136320:CDS
CTTGATTCTCGATCACCTCT+TGG 0.414878 1_3:+68160339 None:intergenic
CAATACACCGTGCAAGGTCC+GGG 0.431500 1_3:-68159182 Msa0136320:CDS
ATTAATACGATGCCGTTTGA+AGG 0.465904 1_3:+68160441 None:intergenic
AACATTTGCTTCATGACTAC+AGG 0.477196 1_3:+68158764 None:intergenic
GAATCATTGGTACAATCATC+AGG 0.481166 1_3:+68158900 None:intergenic
TTTGCGCCCCGGACCTTGCA+CGG 0.490890 1_3:+68159175 None:intergenic
GTCTTTCTCTGACGGAATCT+CGG 0.495284 1_3:+68160148 None:intergenic
TTCTTTATGAGAGCAATAGT+TGG 0.506238 1_3:+68159457 None:intergenic
CGCAAACCAAAAGAAGTGTT+CGG 0.506657 1_3:-68159158 Msa0136320:CDS
CGGTGTAATCTCCGTGTCCG+AGG 0.513907 1_3:+68160168 None:intergenic
ATCTTGAAGCCCTAAGAGAA+AGG 0.527404 1_3:-68160241 Msa0136320:CDS
CGAAGCAGCATATTGAGACT+CGG 0.534134 1_3:-68159543 Msa0136320:CDS
CAGGATAATCCAAACAAGAC+TGG 0.541989 1_3:+68158919 None:intergenic
GACGGTGGCGCAAGCAGTCG+AGG 0.556072 1_3:-68160312 Msa0136320:CDS
TGGTATTAGCACATTTGCAA+AGG 0.556981 1_3:+68158815 None:intergenic
CCGCGAAAATTGGCCCTGCA+AGG 0.557879 1_3:-68160078 Msa0136320:intron
TACCAAAGTGGATGATTATG+TGG 0.558275 1_3:-68159434 Msa0136320:intron
AAACGAAAGGAACCTTCAAA+CGG 0.561017 1_3:-68160453 Msa0136320:five_prime_UTR
GAAATTGTGCGAACCTTGCA+GGG 0.562815 1_3:+68160065 None:intergenic
GAGAAGAAGTCTTTCTCTGA+CGG 0.577836 1_3:+68160140 None:intergenic
AATCATCAATACACCGTGCA+AGG 0.579819 1_3:-68159188 Msa0136320:CDS
CGTTGGATCTCCCTCGGACA+CGG 0.582223 1_3:-68160179 Msa0136320:CDS
TACCACATAATCATCCACTT+TGG 0.582640 1_3:+68159432 None:intergenic
CGTAGCCGTTGGATCTCCCT+CGG 0.582964 1_3:-68160185 Msa0136320:CDS
TGGCGGAGAAGCGTAGCCGT+TGG 0.601854 1_3:-68160196 Msa0136320:CDS
CAATCTTTCTTCCAAAACCT+CGG 0.602383 1_3:+68159242 None:intergenic
AATTTAGAAAGACACACAAA+CGG 0.604177 1_3:+68159014 None:intergenic
ACGATCACTTGCTTTAACGA+CGG 0.607682 1_3:+68160118 None:intergenic
CGTCATCGTCGAGACGCTCA+AGG 0.607794 1_3:+68160264 None:intergenic
GGTGTAATCTCCGTGTCCGA+GGG 0.608291 1_3:+68160169 None:intergenic
TACAAGTTACTCAGAATCTG+AGG 0.610014 1_3:+68159116 None:intergenic
GAACTCAAAGTGCAGAGCGA+GGG 0.616104 1_3:-68160410 Msa0136320:five_prime_UTR
GAGCTCTCGTCTTTCCAGGA+GGG 0.622232 1_3:+68159067 None:intergenic
TGAGCTCTCGTCTTTCCAGG+AGG 0.628602 1_3:+68159066 None:intergenic
TGAAATCTATCCCAAACGAA+AGG 0.631935 1_3:-68160466 Msa0136320:five_prime_UTR
CGTGTCCGAGGGAGATCCAA+CGG 0.633581 1_3:+68160180 None:intergenic
TGAACTCAAAGTGCAGAGCG+AGG 0.639978 1_3:-68160411 Msa0136320:five_prime_UTR
AATACACCGTGCAAGGTCCG+GGG 0.655774 1_3:-68159181 Msa0136320:CDS
GATCGAGAATCAAGTACTGA+CGG 0.657827 1_3:-68160330 Msa0136320:CDS
TCTCATAAAGAATACCAAAG+TGG 0.658073 1_3:-68159446 Msa0136320:CDS
GTCATCGTCGAGACGCTCAA+GGG 0.663012 1_3:+68160265 None:intergenic
TTGAAGCCCTAAGAGAAAGG+AGG 0.672465 1_3:-68160238 Msa0136320:CDS
TACTGATGACTTTAGCACCG+AGG 0.705317 1_3:-68159259 Msa0136320:CDS
GCAACAAATGAAGAAAATGG+CGG 0.709368 1_3:-68160213 Msa0136320:CDS
AAGTGTTCGGCAGAGCACAA+CGG 0.717985 1_3:-68159145 Msa0136320:CDS
GGACAAAGGAAAGATCCAAG+AGG 0.724397 1_3:-68160354 Msa0136320:CDS
AAACTTACAAGACACACGAG+TGG 0.733578 1_3:+68158979 None:intergenic
CGAGAATCAAGTACTGACGG+TGG 0.737424 1_3:-68160327 Msa0136320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAACTTTTTAATTTAATAAA+AGG - chr1_3:68158739-68158758 Msa0136320:three_prime_UTR 10.0%
!! GAACTTTATAAAAATATTAT+TGG - chr1_3:68159475-68159494 Msa0136320:CDS 10.0%
!!! GAACTTTTTAATTTAATAAA+AGG - chr1_3:68158739-68158758 Msa0136320:three_prime_UTR 10.0%
!! GAACTTTATAAAAATATTAT+TGG - chr1_3:68159475-68159494 Msa0136320:CDS 10.0%
!! CTCTCAAAAAATAAAATAAA+AGG - chr1_3:68158771-68158790 Msa0136320:three_prime_UTR 15.0%
!! AAAAAATAAAATAAAAGGAG+AGG - chr1_3:68158776-68158795 Msa0136320:three_prime_UTR 15.0%
!!! AAGAATTTTAATTTAAACGT+AGG - chr1_3:68159453-68159472 Msa0136320:CDS 15.0%
!! CTCTCAAAAAATAAAATAAA+AGG - chr1_3:68158771-68158790 Msa0136320:three_prime_UTR 15.0%
!! AAAAAATAAAATAAAAGGAG+AGG - chr1_3:68158776-68158795 Msa0136320:three_prime_UTR 15.0%
!!! AAGAATTTTAATTTAAACGT+AGG - chr1_3:68159453-68159472 Msa0136320:CDS 15.0%
!! ACATGTGATTACATAATTAT+CGG - chr1_3:68159339-68159358 Msa0136320:intron 20.0%
!! TAGAATTATCTGTTTATCAT+TGG - chr1_3:68159571-68159590 Msa0136320:CDS 20.0%
!! TATTGACATGAATTCTATAT+TGG - chr1_3:68159917-68159936 Msa0136320:intron 20.0%
!!! TGTCTAAATTTTGTTTTTGT+TGG - chr1_3:68160250-68160269 Msa0136320:CDS 20.0%
!!! CTTTCTAAATTTAGTTTTGT+TGG - chr1_3:68160285-68160304 Msa0136320:CDS 20.0%
!! ACATGTGATTACATAATTAT+CGG - chr1_3:68159339-68159358 Msa0136320:intron 20.0%
!! TAGAATTATCTGTTTATCAT+TGG - chr1_3:68159571-68159590 Msa0136320:CDS 20.0%
!! TATTGACATGAATTCTATAT+TGG - chr1_3:68159917-68159936 Msa0136320:intron 20.0%
!!! TGTCTAAATTTTGTTTTTGT+TGG - chr1_3:68160250-68160269 Msa0136320:CDS 20.0%
!!! CTTTCTAAATTTAGTTTTGT+TGG - chr1_3:68160285-68160304 Msa0136320:CDS 20.0%
! AATTATGTAATCACATGTGT+AGG + chr1_3:68159337-68159356 None:intergenic 25.0%
! AATTATCTGTTTATCATTGG+AGG - chr1_3:68159574-68159593 Msa0136320:CDS 25.0%
! ATTATCTGTTTATCATTGGA+GGG - chr1_3:68159575-68159594 Msa0136320:intron 25.0%
! ATATAAAAACTGTAAGTCTG+CGG + chr1_3:68159645-68159664 None:intergenic 25.0%
! TACTAACATCAAGATGATAA+TGG - chr1_3:68159970-68159989 Msa0136320:intron 25.0%
!!! ATGATAATGGTATGTGTTTT+AGG - chr1_3:68159983-68160002 Msa0136320:intron 25.0%
! AATTTAGAAAGACACACAAA+CGG + chr1_3:68160276-68160295 None:intergenic 25.0%
! CTTATAGTGATTAGAATCAT+TGG + chr1_3:68160403-68160422 None:intergenic 25.0%
!!! TAATACTAAGAGTTTTGAGA+TGG - chr1_3:68160423-68160442 Msa0136320:five_prime_UTR 25.0%
! TACTGCATAAAAGTAATTCT+TGG + chr1_3:68160495-68160514 None:intergenic 25.0%
! AATTATGTAATCACATGTGT+AGG + chr1_3:68159337-68159356 None:intergenic 25.0%
! AATTATCTGTTTATCATTGG+AGG - chr1_3:68159574-68159593 Msa0136320:CDS 25.0%
! ATTATCTGTTTATCATTGGA+GGG - chr1_3:68159575-68159594 Msa0136320:intron 25.0%
! ATATAAAAACTGTAAGTCTG+CGG + chr1_3:68159645-68159664 None:intergenic 25.0%
! TACTAACATCAAGATGATAA+TGG - chr1_3:68159970-68159989 Msa0136320:intron 25.0%
!!! ATGATAATGGTATGTGTTTT+AGG - chr1_3:68159983-68160002 Msa0136320:intron 25.0%
! AATTTAGAAAGACACACAAA+CGG + chr1_3:68160276-68160295 None:intergenic 25.0%
! CTTATAGTGATTAGAATCAT+TGG + chr1_3:68160403-68160422 None:intergenic 25.0%
!!! TAATACTAAGAGTTTTGAGA+TGG - chr1_3:68160423-68160442 Msa0136320:five_prime_UTR 25.0%
! TACTGCATAAAAGTAATTCT+TGG + chr1_3:68160495-68160514 None:intergenic 25.0%
AGATCTAGGAGTGAAAAAAA+TGG - chr1_3:68158912-68158931 Msa0136320:three_prime_UTR 30.0%
TGATTACATAATTATCGGTG+TGG - chr1_3:68159344-68159363 Msa0136320:intron 30.0%
CGGAATTCAAATTCAAATTG+TGG + chr1_3:68159625-68159644 None:intergenic 30.0%
! TATTTTCTTTCGCTGAAATG+AGG - chr1_3:68159662-68159681 Msa0136320:intron 30.0%
! GGACAAGCATTTAAGTATTT+TGG - chr1_3:68159721-68159740 Msa0136320:intron 30.0%
TTCTTTATGAGAGCAATAGT+TGG + chr1_3:68159833-68159852 None:intergenic 30.0%
TCTCATAAAGAATACCAAAG+TGG - chr1_3:68159841-68159860 Msa0136320:intron 30.0%
ATTATGTGGTATGTGCTTAA+AGG - chr1_3:68159867-68159886 Msa0136320:intron 30.0%
ACATGAATTCTATATTGGAC+AGG - chr1_3:68159922-68159941 Msa0136320:intron 30.0%
CATGAATTCTATATTGGACA+GGG - chr1_3:68159923-68159942 Msa0136320:intron 30.0%
!! ATAATGGTATGTGTTTTAGG+TGG - chr1_3:68159986-68160005 Msa0136320:intron 30.0%
!! TAATGGTATGTGTTTTAGGT+GGG - chr1_3:68159987-68160006 Msa0136320:intron 30.0%
! TAAAGCTCAAGTCATCTTTT+TGG - chr1_3:68160070-68160089 Msa0136320:intron 30.0%
AGATCTAGGAGTGAAAAAAA+TGG - chr1_3:68158912-68158931 Msa0136320:three_prime_UTR 30.0%
TGATTACATAATTATCGGTG+TGG - chr1_3:68159344-68159363 Msa0136320:intron 30.0%
CGGAATTCAAATTCAAATTG+TGG + chr1_3:68159625-68159644 None:intergenic 30.0%
! TATTTTCTTTCGCTGAAATG+AGG - chr1_3:68159662-68159681 Msa0136320:intron 30.0%
! GGACAAGCATTTAAGTATTT+TGG - chr1_3:68159721-68159740 Msa0136320:intron 30.0%
TTCTTTATGAGAGCAATAGT+TGG + chr1_3:68159833-68159852 None:intergenic 30.0%
TCTCATAAAGAATACCAAAG+TGG - chr1_3:68159841-68159860 Msa0136320:intron 30.0%
ATTATGTGGTATGTGCTTAA+AGG - chr1_3:68159867-68159886 Msa0136320:intron 30.0%
ACATGAATTCTATATTGGAC+AGG - chr1_3:68159922-68159941 Msa0136320:intron 30.0%
CATGAATTCTATATTGGACA+GGG - chr1_3:68159923-68159942 Msa0136320:intron 30.0%
!! ATAATGGTATGTGTTTTAGG+TGG - chr1_3:68159986-68160005 Msa0136320:intron 30.0%
!! TAATGGTATGTGTTTTAGGT+GGG - chr1_3:68159987-68160006 Msa0136320:intron 30.0%
! TAAAGCTCAAGTCATCTTTT+TGG - chr1_3:68160070-68160089 Msa0136320:intron 30.0%
TGAAATCTATCCCAAACGAA+AGG - chr1_3:68158821-68158840 Msa0136320:three_prime_UTR 35.0%
TTTGAAGGTTCCTTTCGTTT+GGG + chr1_3:68158834-68158853 None:intergenic 35.0%
AAACGAAAGGAACCTTCAAA+CGG - chr1_3:68158834-68158853 Msa0136320:three_prime_UTR 35.0%
! ATTAATACGATGCCGTTTGA+AGG + chr1_3:68158849-68158868 None:intergenic 35.0%
GGAGTGAAAAAAATGGACAA+AGG - chr1_3:68158919-68158938 Msa0136320:three_prime_UTR 35.0%
GCTGCAACAAATGAAGAAAA+TGG - chr1_3:68159071-68159090 Msa0136320:three_prime_UTR 35.0%
GCAACAAATGAAGAAAATGG+CGG - chr1_3:68159074-68159093 Msa0136320:three_prime_UTR 35.0%
ATATTATTGGCAGTGCATAC+TGG - chr1_3:68159488-68159507 Msa0136320:CDS 35.0%
TTGAGCTTCTCAGCTAAAAA+TGG + chr1_3:68159797-68159816 None:intergenic 35.0%
TACCAAAGTGGATGATTATG+TGG - chr1_3:68159853-68159872 Msa0136320:intron 35.0%
TACCACATAATCATCCACTT+TGG + chr1_3:68159858-68159877 None:intergenic 35.0%
CAATCTTTCTTCCAAAACCT+CGG + chr1_3:68160048-68160067 None:intergenic 35.0%
TACAAGTTACTCAGAATCTG+AGG + chr1_3:68160174-68160193 None:intergenic 35.0%
!! TATCAATTGCCAGTCTTGTT+TGG - chr1_3:68160359-68160378 Msa0136320:CDS 35.0%
GAATCATTGGTACAATCATC+AGG + chr1_3:68160390-68160409 None:intergenic 35.0%
GCAAAGGACAAACACATTAA+AGG + chr1_3:68160459-68160478 None:intergenic 35.0%
TGGTATTAGCACATTTGCAA+AGG + chr1_3:68160475-68160494 None:intergenic 35.0%
AACATTTGCTTCATGACTAC+AGG + chr1_3:68160526-68160545 None:intergenic 35.0%
TGAAATCTATCCCAAACGAA+AGG - chr1_3:68158821-68158840 Msa0136320:three_prime_UTR 35.0%
TTTGAAGGTTCCTTTCGTTT+GGG + chr1_3:68158834-68158853 None:intergenic 35.0%
AAACGAAAGGAACCTTCAAA+CGG - chr1_3:68158834-68158853 Msa0136320:three_prime_UTR 35.0%
! ATTAATACGATGCCGTTTGA+AGG + chr1_3:68158849-68158868 None:intergenic 35.0%
GGAGTGAAAAAAATGGACAA+AGG - chr1_3:68158919-68158938 Msa0136320:three_prime_UTR 35.0%
GCTGCAACAAATGAAGAAAA+TGG - chr1_3:68159071-68159090 Msa0136320:three_prime_UTR 35.0%
GCAACAAATGAAGAAAATGG+CGG - chr1_3:68159074-68159093 Msa0136320:three_prime_UTR 35.0%
ATATTATTGGCAGTGCATAC+TGG - chr1_3:68159488-68159507 Msa0136320:CDS 35.0%
TTGAGCTTCTCAGCTAAAAA+TGG + chr1_3:68159797-68159816 None:intergenic 35.0%
TACCAAAGTGGATGATTATG+TGG - chr1_3:68159853-68159872 Msa0136320:intron 35.0%
TACCACATAATCATCCACTT+TGG + chr1_3:68159858-68159877 None:intergenic 35.0%
CAATCTTTCTTCCAAAACCT+CGG + chr1_3:68160048-68160067 None:intergenic 35.0%
TACAAGTTACTCAGAATCTG+AGG + chr1_3:68160174-68160193 None:intergenic 35.0%
!! TATCAATTGCCAGTCTTGTT+TGG - chr1_3:68160359-68160378 Msa0136320:CDS 35.0%
GAATCATTGGTACAATCATC+AGG + chr1_3:68160390-68160409 None:intergenic 35.0%
GCAAAGGACAAACACATTAA+AGG + chr1_3:68160459-68160478 None:intergenic 35.0%
TGGTATTAGCACATTTGCAA+AGG + chr1_3:68160475-68160494 None:intergenic 35.0%
AACATTTGCTTCATGACTAC+AGG + chr1_3:68160526-68160545 None:intergenic 35.0%
GTTTGAAGGTTCCTTTCGTT+TGG + chr1_3:68158835-68158854 None:intergenic 40.0%
!! GATCGAGAATCAAGTACTGA+CGG - chr1_3:68158957-68158976 Msa0136320:three_prime_UTR 40.0%
ATCTTGAAGCCCTAAGAGAA+AGG - chr1_3:68159046-68159065 Msa0136320:three_prime_UTR 40.0%
GAGAAGAAGTCTTTCTCTGA+CGG + chr1_3:68159150-68159169 None:intergenic 40.0%
ACGATCACTTGCTTTAACGA+CGG + chr1_3:68159172-68159191 None:intergenic 40.0%
GTCATTTCTTCCGCGAAAAT+TGG - chr1_3:68159199-68159218 Msa0136320:CDS 40.0%
GTAATCACATGTGTAGGTGT+CGG + chr1_3:68159331-68159350 None:intergenic 40.0%
! TGCATTCAGTTTGCTTAGCT+TGG - chr1_3:68159425-68159444 Msa0136320:intron 40.0%
!! TAGGTGGGTTTTGATGAACT+TGG - chr1_3:68160002-68160021 Msa0136320:intron 40.0%
!!! CGAGGTTTTGGAAGAAAGAT+TGG - chr1_3:68160046-68160065 Msa0136320:intron 40.0%
! AGCTCAAGTCATCTTTTTGG+AGG - chr1_3:68160073-68160092 Msa0136320:intron 40.0%
! GCTCAAGTCATCTTTTTGGA+GGG - chr1_3:68160074-68160093 Msa0136320:intron 40.0%
AATCATCAATACACCGTGCA+AGG - chr1_3:68160099-68160118 Msa0136320:CDS 40.0%
!! CGCAAACCAAAAGAAGTGTT+CGG - chr1_3:68160129-68160148 Msa0136320:CDS 40.0%
!! TTTTATTTACGTTGCCCTCC+TGG - chr1_3:68160206-68160225 Msa0136320:CDS 40.0%
AAACTTACAAGACACACGAG+TGG + chr1_3:68160311-68160330 None:intergenic 40.0%
CAGGATAATCCAAACAAGAC+TGG + chr1_3:68160371-68160390 None:intergenic 40.0%
GTTTGAAGGTTCCTTTCGTT+TGG + chr1_3:68158835-68158854 None:intergenic 40.0%
!! GATCGAGAATCAAGTACTGA+CGG - chr1_3:68158957-68158976 Msa0136320:three_prime_UTR 40.0%
ATCTTGAAGCCCTAAGAGAA+AGG - chr1_3:68159046-68159065 Msa0136320:three_prime_UTR 40.0%
GAGAAGAAGTCTTTCTCTGA+CGG + chr1_3:68159150-68159169 None:intergenic 40.0%
ACGATCACTTGCTTTAACGA+CGG + chr1_3:68159172-68159191 None:intergenic 40.0%
GTCATTTCTTCCGCGAAAAT+TGG - chr1_3:68159199-68159218 Msa0136320:CDS 40.0%
GTAATCACATGTGTAGGTGT+CGG + chr1_3:68159331-68159350 None:intergenic 40.0%
! TGCATTCAGTTTGCTTAGCT+TGG - chr1_3:68159425-68159444 Msa0136320:intron 40.0%
!! TAGGTGGGTTTTGATGAACT+TGG - chr1_3:68160002-68160021 Msa0136320:intron 40.0%
!!! CGAGGTTTTGGAAGAAAGAT+TGG - chr1_3:68160046-68160065 Msa0136320:intron 40.0%
! AGCTCAAGTCATCTTTTTGG+AGG - chr1_3:68160073-68160092 Msa0136320:intron 40.0%
! GCTCAAGTCATCTTTTTGGA+GGG - chr1_3:68160074-68160093 Msa0136320:intron 40.0%
AATCATCAATACACCGTGCA+AGG - chr1_3:68160099-68160118 Msa0136320:CDS 40.0%
!! CGCAAACCAAAAGAAGTGTT+CGG - chr1_3:68160129-68160148 Msa0136320:CDS 40.0%
!! TTTTATTTACGTTGCCCTCC+TGG - chr1_3:68160206-68160225 Msa0136320:CDS 40.0%
AAACTTACAAGACACACGAG+TGG + chr1_3:68160311-68160330 None:intergenic 40.0%
CAGGATAATCCAAACAAGAC+TGG + chr1_3:68160371-68160390 None:intergenic 40.0%
GGACAAAGGAAAGATCCAAG+AGG - chr1_3:68158933-68158952 Msa0136320:three_prime_UTR 45.0%
CTTGATTCTCGATCACCTCT+TGG + chr1_3:68158951-68158970 None:intergenic 45.0%
TTGAAGCCCTAAGAGAAAGG+AGG - chr1_3:68159049-68159068 Msa0136320:three_prime_UTR 45.0%
TTGCAGCCTCCTTTCTCTTA+GGG + chr1_3:68159058-68159077 None:intergenic 45.0%
GTCTTTCTCTGACGGAATCT+CGG + chr1_3:68159142-68159161 None:intergenic 45.0%
GAAATTGTGCGAACCTTGCA+GGG + chr1_3:68159225-68159244 None:intergenic 45.0%
TGTGCTCACAGTTCATTCAC+AGG - chr1_3:68159694-68159713 Msa0136320:intron 45.0%
CACAGTTCATTCACAGGTTG+TGG - chr1_3:68159700-68159719 Msa0136320:intron 45.0%
CGAAGCAGCATATTGAGACT+CGG - chr1_3:68159744-68159763 Msa0136320:intron 45.0%
!! GTGGGTTTTGATGAACTTGG+TGG - chr1_3:68160005-68160024 Msa0136320:intron 45.0%
TACTGATGACTTTAGCACCG+AGG - chr1_3:68160028-68160047 Msa0136320:intron 45.0%
!! TGACTTTAGCACCGAGGTTT+TGG - chr1_3:68160034-68160053 Msa0136320:intron 45.0%
GGACAAAGGAAAGATCCAAG+AGG - chr1_3:68158933-68158952 Msa0136320:three_prime_UTR 45.0%
CTTGATTCTCGATCACCTCT+TGG + chr1_3:68158951-68158970 None:intergenic 45.0%
TTGAAGCCCTAAGAGAAAGG+AGG - chr1_3:68159049-68159068 Msa0136320:three_prime_UTR 45.0%
TTGCAGCCTCCTTTCTCTTA+GGG + chr1_3:68159058-68159077 None:intergenic 45.0%
GTCTTTCTCTGACGGAATCT+CGG + chr1_3:68159142-68159161 None:intergenic 45.0%
GAAATTGTGCGAACCTTGCA+GGG + chr1_3:68159225-68159244 None:intergenic 45.0%
TGTGCTCACAGTTCATTCAC+AGG - chr1_3:68159694-68159713 Msa0136320:intron 45.0%
CACAGTTCATTCACAGGTTG+TGG - chr1_3:68159700-68159719 Msa0136320:intron 45.0%
CGAAGCAGCATATTGAGACT+CGG - chr1_3:68159744-68159763 Msa0136320:intron 45.0%
!! GTGGGTTTTGATGAACTTGG+TGG - chr1_3:68160005-68160024 Msa0136320:intron 45.0%
TACTGATGACTTTAGCACCG+AGG - chr1_3:68160028-68160047 Msa0136320:intron 45.0%
!! TGACTTTAGCACCGAGGTTT+TGG - chr1_3:68160034-68160053 Msa0136320:intron 45.0%
TGAACTCAAAGTGCAGAGCG+AGG - chr1_3:68158876-68158895 Msa0136320:three_prime_UTR 50.0%
GAACTCAAAGTGCAGAGCGA+GGG - chr1_3:68158877-68158896 Msa0136320:three_prime_UTR 50.0%
GGAGAGAGAGAGAGAGATCT+AGG - chr1_3:68158898-68158917 Msa0136320:three_prime_UTR 50.0%
!! CGAGAATCAAGTACTGACGG+TGG - chr1_3:68158960-68158979 Msa0136320:three_prime_UTR 50.0%
GTTGCAGCCTCCTTTCTCTT+AGG + chr1_3:68159059-68159078 None:intergenic 50.0%
CGAAATTGTGCGAACCTTGC+AGG + chr1_3:68159226-68159245 None:intergenic 50.0%
CGGTGTGGATGTGTAGTGTT+AGG - chr1_3:68159359-68159378 Msa0136320:intron 50.0%
TCAATACACCGTGCAAGGTC+CGG - chr1_3:68160104-68160123 Msa0136320:CDS 50.0%
!!! ACTTCTTTTGGTTTGCGCCC+CGG + chr1_3:68160126-68160145 None:intergenic 50.0%
! GCTCTGCCGAACACTTCTTT+TGG + chr1_3:68160138-68160157 None:intergenic 50.0%
AAGTGTTCGGCAGAGCACAA+CGG - chr1_3:68160142-68160161 Msa0136320:CDS 50.0%
ATCTGAGCTCTCGTCTTTCC+AGG + chr1_3:68160227-68160246 None:intergenic 50.0%
TGAACTCAAAGTGCAGAGCG+AGG - chr1_3:68158876-68158895 Msa0136320:three_prime_UTR 50.0%
GAACTCAAAGTGCAGAGCGA+GGG - chr1_3:68158877-68158896 Msa0136320:three_prime_UTR 50.0%
GGAGAGAGAGAGAGAGATCT+AGG - chr1_3:68158898-68158917 Msa0136320:three_prime_UTR 50.0%
!! CGAGAATCAAGTACTGACGG+TGG - chr1_3:68158960-68158979 Msa0136320:three_prime_UTR 50.0%
GTTGCAGCCTCCTTTCTCTT+AGG + chr1_3:68159059-68159078 None:intergenic 50.0%
CGAAATTGTGCGAACCTTGC+AGG + chr1_3:68159226-68159245 None:intergenic 50.0%
CGGTGTGGATGTGTAGTGTT+AGG - chr1_3:68159359-68159378 Msa0136320:intron 50.0%
TCAATACACCGTGCAAGGTC+CGG - chr1_3:68160104-68160123 Msa0136320:CDS 50.0%
!!! ACTTCTTTTGGTTTGCGCCC+CGG + chr1_3:68160126-68160145 None:intergenic 50.0%
! GCTCTGCCGAACACTTCTTT+TGG + chr1_3:68160138-68160157 None:intergenic 50.0%
AAGTGTTCGGCAGAGCACAA+CGG - chr1_3:68160142-68160161 Msa0136320:CDS 50.0%
ATCTGAGCTCTCGTCTTTCC+AGG + chr1_3:68160227-68160246 None:intergenic 50.0%
GTCATCGTCGAGACGCTCAA+GGG + chr1_3:68159025-68159044 None:intergenic 55.0%
GGTGTAATCTCCGTGTCCGA+GGG + chr1_3:68159121-68159140 None:intergenic 55.0%
! CCTTGCAGGGCCAATTTTCG+CGG + chr1_3:68159212-68159231 None:intergenic 55.0%
CAATACACCGTGCAAGGTCC+GGG - chr1_3:68160105-68160124 Msa0136320:CDS 55.0%
AATACACCGTGCAAGGTCCG+GGG - chr1_3:68160106-68160125 Msa0136320:CDS 55.0%
GAGCTCTCGTCTTTCCAGGA+GGG + chr1_3:68160223-68160242 None:intergenic 55.0%
TGAGCTCTCGTCTTTCCAGG+AGG + chr1_3:68160224-68160243 None:intergenic 55.0%
GTCATCGTCGAGACGCTCAA+GGG + chr1_3:68159025-68159044 None:intergenic 55.0%
GGTGTAATCTCCGTGTCCGA+GGG + chr1_3:68159121-68159140 None:intergenic 55.0%
! CCTTGCAGGGCCAATTTTCG+CGG + chr1_3:68159212-68159231 None:intergenic 55.0%
CAATACACCGTGCAAGGTCC+GGG - chr1_3:68160105-68160124 Msa0136320:CDS 55.0%
AATACACCGTGCAAGGTCCG+GGG - chr1_3:68160106-68160125 Msa0136320:CDS 55.0%
GAGCTCTCGTCTTTCCAGGA+GGG + chr1_3:68160223-68160242 None:intergenic 55.0%
TGAGCTCTCGTCTTTCCAGG+AGG + chr1_3:68160224-68160243 None:intergenic 55.0%
CGTCATCGTCGAGACGCTCA+AGG + chr1_3:68159026-68159045 None:intergenic 60.0%
CGTAGCCGTTGGATCTCCCT+CGG - chr1_3:68159102-68159121 Msa0136320:three_prime_UTR 60.0%
CGTGTCCGAGGGAGATCCAA+CGG + chr1_3:68159110-68159129 None:intergenic 60.0%
CGTTGGATCTCCCTCGGACA+CGG - chr1_3:68159108-68159127 Msa0136320:exon 60.0%
CGGTGTAATCTCCGTGTCCG+AGG + chr1_3:68159122-68159141 None:intergenic 60.0%
CCGCGAAAATTGGCCCTGCA+AGG - chr1_3:68159209-68159228 Msa0136320:CDS 60.0%
CGTCATCGTCGAGACGCTCA+AGG + chr1_3:68159026-68159045 None:intergenic 60.0%
CGTAGCCGTTGGATCTCCCT+CGG - chr1_3:68159102-68159121 Msa0136320:three_prime_UTR 60.0%
CGTGTCCGAGGGAGATCCAA+CGG + chr1_3:68159110-68159129 None:intergenic 60.0%
CGTTGGATCTCCCTCGGACA+CGG - chr1_3:68159108-68159127 Msa0136320:exon 60.0%
CGGTGTAATCTCCGTGTCCG+AGG + chr1_3:68159122-68159141 None:intergenic 60.0%
CCGCGAAAATTGGCCCTGCA+AGG - chr1_3:68159209-68159228 Msa0136320:CDS 60.0%
! TGGCGGAGAAGCGTAGCCGT+TGG - chr1_3:68159091-68159110 Msa0136320:three_prime_UTR 65.0%
TTTGCGCCCCGGACCTTGCA+CGG + chr1_3:68160115-68160134 None:intergenic 65.0%
! TGGCGGAGAAGCGTAGCCGT+TGG - chr1_3:68159091-68159110 Msa0136320:three_prime_UTR 65.0%
TTTGCGCCCCGGACCTTGCA+CGG + chr1_3:68160115-68160134 None:intergenic 65.0%
GACGGTGGCGCAAGCAGTCG+AGG - chr1_3:68158975-68158994 Msa0136320:three_prime_UTR 70.0%
GACGGTGGCGCAAGCAGTCG+AGG - chr1_3:68158975-68158994 Msa0136320:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 68158724 68160585 68158724 ID=Msa0136320;Name=Msa0136320
chr1_3 mRNA 68158724 68160585 68158724 ID=Msa0136320-mRNA-1;Parent=Msa0136320;Name=Msa0136320-mRNA-1;_AED=0.00;_eAED=0.00;_QI=207|1|1|1|1|1|3|398|213
chr1_3 exon 68158724 68159304 68158724 ID=Msa0136320-mRNA-1:exon:23513;Parent=Msa0136320-mRNA-1
chr1_3 exon 68159435 68159593 68159435 ID=Msa0136320-mRNA-1:exon:23512;Parent=Msa0136320-mRNA-1
chr1_3 exon 68160079 68160585 68160079 ID=Msa0136320-mRNA-1:exon:23511;Parent=Msa0136320-mRNA-1
chr1_3 five_prime_UTR 68160379 68160585 68160379 ID=Msa0136320-mRNA-1:five_prime_utr;Parent=Msa0136320-mRNA-1
chr1_3 CDS 68160079 68160378 68160079 ID=Msa0136320-mRNA-1:cds;Parent=Msa0136320-mRNA-1
chr1_3 CDS 68159435 68159593 68159435 ID=Msa0136320-mRNA-1:cds;Parent=Msa0136320-mRNA-1
chr1_3 CDS 68159122 68159304 68159122 ID=Msa0136320-mRNA-1:cds;Parent=Msa0136320-mRNA-1
chr1_3 three_prime_UTR 68158724 68159121 68158724 ID=Msa0136320-mRNA-1:three_prime_utr;Parent=Msa0136320-mRNA-1
Gene Sequence

>Msa0136320

ATGGACAAAGGAAAGATCCAAGAGGTGATCGAGAATCAAGTACTGACGGTGGCGCAAGCAGTCGAGGATAAGATCGACGAAGAGATTGCTGCCCTTGAGCGTCTCGACGATGACGATCTTGAAGCCCTAAGAGAAAGGAGGCTGCAACAAATGAAGAAAATGGCGGAGAAGCGTAGCCGTTGGATCTCCCTCGGACACGGAGATTACACCGAGATTCCGTCAGAGAAAGACTTCTTCTCCGTCGTTAAAGCAAGTGATCGTGTTGTTTGTCATTTCTTCCGCGAAAATTGGCCCTGCAAGGTTGTGGACAAGCATTTAAGTATTTTGGCGAAGCAGCATATTGAGACTCGGTTTGTGAAAATAAATGCTGAAAAAAGTCCATTTTTAGCTGAGAAGCTCAAGATCATTGTTCTTCCAACTATTGCTCTCATAAAGAATACCAAAGTGGATGATTATGTGGTGGGTTTTGATGAACTTGGTGGTACTGATGACTTTAGCACCGAGGTTTTGGAAGAAAGATTGGCTAAAGCTCAAGTCATCTTTTTGGAGGGTGAATCATCAATACACCGTGCAAGGTCCGGGGCGCAAACCAAAAGAAGTGTTCGGCAGAGCACAACGGCTGACTCCTCAGATTCTGAGTAA
Protein sequence

>Msa0136320

MDKGKIQEVIENQVLTVAQAVEDKIDEEIAALERLDDDDLEALRERRLQQMKKMAEKRSRWISLGHGDYTEIPSEKDFFSVVKASDRVVCHFFRENWPCKVVDKHLSILAKQHIETRFVKINAEKSPFLAEKLKIIVLPTIALIKNTKVDDYVVGFDELGGTDDFSTEVLEERLAKAQVIFLEGESSIHRARSGAQTKRSVRQSTTADSSDSE*