Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1465370 | XP_003613091.1 | 98.058 | 103 | 2 | 0 | 1 | 103 | 75 | 177 | 3.80e-65 | 204 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1465370 | G7JXR4 | 98.058 | 103 | 2 | 0 | 1 | 103 | 75 | 177 | 1.82e-65 | 204 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0012440 | Msa1465370 | 0.801432 | 9.031172e-49 | -8.615850e-47 |
| Msa1429420 | Msa1465370 | 0.805910 | 1.062461e-49 | -8.615850e-47 |
| Msa0149560 | Msa1465370 | 0.803013 | 4.268718e-49 | -8.615850e-47 |
| Msa1160400 | Msa1465370 | 0.804915 | 1.717805e-49 | -8.615850e-47 |
| Msa0847990 | Msa1465370 | 0.825222 | 5.273125e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1465370 | MtrunA17_Chr5g0412491 | 98.058 | 103 | 2 | 0 | 1 | 103 | 65 | 167 | 3.87e-69 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAACAAAGAAGTAAATAAAA+AGG | + | tig0045206:87-106 | Msa1465370:CDS | 15.0% |
| !! | AACAAAGAAGTAAATAAAAA+GGG | + | tig0045206:88-107 | Msa1465370:CDS | 15.0% |
| !!! | ACTTCTTTCAAACATTTTTT+GGG | - | tig0045206:139-158 | Msa1465370:intergenic | 20.0% |
| !! | AAAAGGATAAAATAATGATG+AGG | + | tig0045206:239-258 | Msa1465370:CDS | 20.0% |
| !! | AAAGGATAAAATAATGATGA+GGG | + | tig0045206:240-259 | Msa1465370:CDS | 20.0% |
| ! | AGGAACTAAAATTGAAATCA+AGG | + | tig0045206:60-79 | Msa1465370:CDS | 25.0% |
| ! | AACTAAAATTGAAATCAAGG+AGG | + | tig0045206:63-82 | Msa1465370:CDS | 25.0% |
| !!! | CACTTCTTTCAAACATTTTT+TGG | - | tig0045206:140-159 | Msa1465370:intergenic | 25.0% |
| ! | ATATGTTAGATCAAAGCAAA+AGG | + | tig0045206:222-241 | Msa1465370:CDS | 25.0% |
| ! | AAGGATAAAATAATGATGAG+GGG | + | tig0045206:241-260 | Msa1465370:CDS | 25.0% |
| ATTATAAGGATGAGAATGGA+AGG | + | tig0045206:37-56 | Msa1465370:CDS | 30.0% | |
| !!! | CATTTTTTGGGTCTAACATT+TGG | - | tig0045206:127-146 | Msa1465370:intergenic | 30.0% |
| AAGAAGTGAAGAAGATTGAT+TGG | + | tig0045206:152-171 | Msa1465370:CDS | 30.0% | |
| AGAAGTGAAGAAGATTGATT+GGG | + | tig0045206:153-172 | Msa1465370:CDS | 30.0% | |
| ! | CAAAATTAGAGCACTTTCTG+AGG | + | tig0045206:12-31 | Msa1465370:CDS | 35.0% |
| !! | CACTTTCTGAGGTGATTATA+AGG | + | tig0045206:23-42 | Msa1465370:CDS | 35.0% |
| GGTGATTATAAGGATGAGAA+TGG | + | tig0045206:33-52 | Msa1465370:CDS | 35.0% | |
| CTAACATATGATCCTTTCAC+AGG | - | tig0045206:211-230 | Msa1465370:intergenic | 35.0% | |
| GGATAAGAAGCTACTTCAAT+TGG | + | tig0045206:279-298 | Msa1465370:CDS | 35.0% | |
| ATAAGGATGAGAATGGAAGG+AGG | + | tig0045206:40-59 | Msa1465370:CDS | 40.0% | |
| TTGATTGGGAGAAGAGTTTG+AGG | + | tig0045206:167-186 | Msa1465370:CDS | 40.0% | |
| TGATTGGGAGAAGAGTTTGA+GGG | + | tig0045206:168-187 | Msa1465370:CDS | 40.0% | |
| ATGATCCTTTCACAGGAACA+GGG | - | tig0045206:204-223 | Msa1465370:intergenic | 40.0% | |
| TATGATCCTTTCACAGGAAC+AGG | - | tig0045206:205-224 | Msa1465370:intergenic | 40.0% | |
| AGAAGCTACTTCAATTGGTG+TGG | + | tig0045206:284-303 | Msa1465370:CDS | 40.0% | |
| GCTACTTCAATTGGTGTGGA+AGG | + | tig0045206:288-307 | Msa1465370:CDS | 45.0% | |
| TCAAGCCCTGTTCCTGTGAA+AGG | + | tig0045206:196-215 | Msa1465370:CDS | 50.0% | |
| GAGGGGAAAGAATGCAAGTG+AGG | + | tig0045206:258-277 | Msa1465370:CDS | 50.0% | |
| TGGGAGAAGAGTTTGAGGGC+AGG | + | tig0045206:172-191 | Msa1465370:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0045206 | gene | 4 | 315 | 4 | ID=Msa1465370;Name=Msa1465370 |
| tig0045206 | mRNA | 4 | 315 | 4 | ID=Msa1465370-mRNA-1;Parent=Msa1465370;Name=Msa1465370-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|103 |
| tig0045206 | exon | 4 | 315 | 4 | ID=Msa1465370-mRNA-1:exon:24779;Parent=Msa1465370-mRNA-1 |
| tig0045206 | CDS | 4 | 315 | 4 | ID=Msa1465370-mRNA-1:cds;Parent=Msa1465370-mRNA-1 |
| Gene Sequence |
| Protein sequence |
