Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000619.t1 | XP_003604244.1 | 100 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 5.00E-133 | 483.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000619.t1 | Q9XGM1 | 75.2 | 262 | 61 | 2 | 1 | 259 | 1 | 261 | 7.4e-101 | 368.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000619.t1 | G7JEF1 | 100.0 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 3.6e-133 | 483.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene000619 | MS.gene034283 | PPI |
| MS.gene024260 | MS.gene000619 | PPI |
| MS.gene000619 | MS.gene28505 | PPI |
| MS.gene000619 | MS.gene98140 | PPI |
| MS.gene000619 | MS.gene011917 | PPI |
| MS.gene000619 | MS.gene34307 | PPI |
| MS.gene000619 | MS.gene44998 | PPI |
| MS.gene000619 | MS.gene87386 | PPI |
| MS.gene000619 | MS.gene72597 | PPI |
| MS.gene000619 | MS.gene54962 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000619.t1 | MTR_4g007030 | 100.000 | 259 | 0 | 0 | 1 | 259 | 1 | 259 | 0.0 | 524 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000619.t1 | AT3G58730 | 79.389 | 262 | 50 | 2 | 1 | 259 | 1 | 261 | 1.47e-152 | 426 |
Find 61 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTAGGTTGAAGAAGATTC+AGG | 0.251278 | 4.3:+88415257 | MS.gene000619:CDS |
| GAATACTATTAGTTACATTA+AGG | 0.278798 | 4.3:+88415203 | MS.gene000619:CDS |
| GTGTCGTGTTGCTTATATTA+AGG | 0.324047 | 4.3:+88415059 | MS.gene000619:CDS |
| TTGAAAGGGAGGATTTCTTT+AGG | 0.370172 | 4.3:+88415241 | MS.gene000619:CDS |
| AACTTGTTGGCTGGTACGAC+TGG | 0.385007 | 4.3:+88415384 | MS.gene000619:CDS |
| GACAATGCTTGGAGTGGTTA+AGG | 0.388176 | 4.3:+88414693 | MS.gene000619:CDS |
| TGAAACCTCTCACAACTTGT+TGG | 0.400530 | 4.3:+88415371 | MS.gene000619:CDS |
| AGAAGATTCAGGGTTATAAG+AGG | 0.430053 | 4.3:+88415268 | MS.gene000619:CDS |
| TGTTTCAACAAAGGAATCAA+TGG | 0.435503 | 4.3:+88414807 | MS.gene000619:CDS |
| GTGAGTTGGATGAGCTTGAA+AGG | 0.439414 | 4.3:+88415226 | MS.gene000619:CDS |
| AATACTATTAGTTACATTAA+GGG | 0.450457 | 4.3:+88415204 | MS.gene000619:CDS |
| TAGTTACATTAAGGGTGAGT+TGG | 0.451344 | 4.3:+88415212 | MS.gene000619:CDS |
| AAAGGAAGATGGTGATTGCT+AGG | 0.464882 | 4.3:+88415301 | MS.gene000619:CDS |
| TGGAGTGGTTAAGGCACGTT+TGG | 0.467712 | 4.3:+88414702 | MS.gene000619:CDS |
| GTTTCAACAAAGGAATCAAT+GGG | 0.471657 | 4.3:+88414808 | MS.gene000619:CDS |
| ACCTCTCACAACTTGTTGGC+TGG | 0.473238 | 4.3:+88415375 | MS.gene000619:CDS |
| TGCTTATATTAAGGCGATTG+AGG | 0.474951 | 4.3:+88415068 | MS.gene000619:CDS |
| AAAAGTTACAATTGCAGCAA+GGG | 0.488148 | 4.3:+88415340 | MS.gene000619:CDS |
| CTAATAGTATTCTCCAACCT+CGG | 0.495607 | 4.3:-88415192 | None:intergenic |
| GTTGGATGAGCTTGAAAGGG+AGG | 0.499311 | 4.3:+88415230 | MS.gene000619:CDS |
| GTGGTTAAGGCACGTTTGGT+TGG | 0.501885 | 4.3:+88414706 | MS.gene000619:CDS |
| TTTAGGTTGAAGAAGATTCA+GGG | 0.502528 | 4.3:+88415258 | MS.gene000619:CDS |
| TAATAGCATCATCAAGGGTA+AGG | 0.507164 | 4.3:-88415125 | None:intergenic |
| TGAGTTGGATGAGCTTGAAA+GGG | 0.514528 | 4.3:+88415227 | MS.gene000619:CDS |
| ATTGGCTAGAGGTGGTCAGC+AGG | 0.515670 | 4.3:+88415029 | MS.gene000619:CDS |
| GATTCAGGGTTATAAGAGGA+GGG | 0.517039 | 4.3:+88415272 | MS.gene000619:CDS |
| TCAGCACTGTAATCAAACTT+TGG | 0.538953 | 4.3:-88414976 | None:intergenic |
| TAGTCTTAATAGCATCATCA+AGG | 0.542669 | 4.3:-88415131 | None:intergenic |
| GAAAAGTTACAATTGCAGCA+AGG | 0.543936 | 4.3:+88415339 | MS.gene000619:CDS |
| AGATTCAGGGTTATAAGAGG+AGG | 0.545100 | 4.3:+88415271 | MS.gene000619:CDS |
| GAGGGAAATTGAAAGGAAGA+TGG | 0.546430 | 4.3:+88415290 | MS.gene000619:CDS |
| GAATGTTGTTAAGCCGAGGT+TGG | 0.546577 | 4.3:+88415179 | MS.gene000619:CDS |
| GTCCCCACAGTGACAATGCT+TGG | 0.555756 | 4.3:+88414682 | MS.gene000619:CDS |
| ATCATCAAGGGTAAGGAAAG+AGG | 0.556678 | 4.3:-88415118 | None:intergenic |
| ATAAGAGGAGGGAAATTGAA+AGG | 0.563654 | 4.3:+88415283 | MS.gene000619:CDS |
| TCTCGTACTGAGAATGTGGC+AGG | 0.566710 | 4.3:+88414943 | MS.gene000619:CDS |
| ATGTTAAAGAAGCTTCACTC+AGG | 0.568588 | 4.3:+88414914 | MS.gene000619:CDS |
| CTATTAAGACTACTAACCGT+AGG | 0.570720 | 4.3:+88415142 | MS.gene000619:CDS |
| ACTCCAAGCATTGTCACTGT+GGG | 0.583853 | 4.3:-88414685 | None:intergenic |
| ACTGAGGCTAAGTATGTAGC+CGG | 0.591330 | 4.3:+88414862 | MS.gene000619:CDS |
| GCAATCACAACGTTTGAATG+TGG | 0.591655 | 4.3:+88414660 | MS.gene000619:CDS |
| TTCTTCATTTGCATTAACTG+AGG | 0.593026 | 4.3:+88414846 | MS.gene000619:CDS |
| CGTTTGGTTGGAGCAACAAG+AGG | 0.602491 | 4.3:+88414718 | MS.gene000619:CDS |
| TTGAGAATGTTGTTAAGCCG+AGG | 0.609957 | 4.3:+88415175 | MS.gene000619:CDS |
| AAGGAAGATGGTGATTGCTA+GGG | 0.610095 | 4.3:+88415302 | MS.gene000619:CDS |
| CACTCCAAGCATTGTCACTG+TGG | 0.610571 | 4.3:-88414686 | None:intergenic |
| AAGTTTGATTACAGTGCTGA+CGG | 0.611337 | 4.3:+88414979 | MS.gene000619:CDS |
| TCTCAAGAGCATTAACCCTA+CGG | 0.615251 | 4.3:-88415158 | None:intergenic |
| AAAGTTACAATTGCAGCAAG+GGG | 0.616721 | 4.3:+88415341 | MS.gene000619:CDS |
| AGTCTTAATAGCATCATCAA+GGG | 0.622324 | 4.3:-88415130 | None:intergenic |
| GACGTGCTTGATGTTATCAC+CGG | 0.624967 | 4.3:-88414881 | None:intergenic |
| ACGTTCTCGTACTGAGAATG+TGG | 0.627068 | 4.3:+88414939 | MS.gene000619:CDS |
| CACAGTGACAATGCTTGGAG+TGG | 0.627138 | 4.3:+88414687 | MS.gene000619:CDS |
| TATTAAGACTACTAACCGTA+GGG | 0.628796 | 4.3:+88415143 | MS.gene000619:CDS |
| ACCAGCCAACAAGTTGTGAG+AGG | 0.637543 | 4.3:-88415376 | None:intergenic |
| TGTTAAAGAAGCTTCACTCA+GGG | 0.649586 | 4.3:+88414915 | MS.gene000619:CDS |
| CTGACTGGATTGGCTAGAGG+TGG | 0.655401 | 4.3:+88415021 | MS.gene000619:CDS |
| TGATTACAGTGCTGACGGTG+AGG | 0.656119 | 4.3:+88414984 | MS.gene000619:CDS |
| GATCTGACTGGATTGGCTAG+AGG | 0.672119 | 4.3:+88415018 | MS.gene000619:CDS |
| CTCCAAGCATTGTCACTGTG+GGG | 0.709998 | 4.3:-88414684 | None:intergenic |
| TGGTACGACTGGAAAAGACG+AGG | 0.748180 | 4.3:+88415395 | MS.gene000619:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATACTATTAGTTACATTAA+GGG | + | chr4.3:88415204-88415223 | MS.gene000619:CDS | 15.0% |
| !! | GAATACTATTAGTTACATTA+AGG | + | chr4.3:88415203-88415222 | MS.gene000619:CDS | 20.0% |
| ! | GAAAAAGATTGTTTCAACAA+AGG | + | chr4.3:88414798-88414817 | MS.gene000619:CDS | 25.0% |
| AAAAGTTACAATTGCAGCAA+GGG | + | chr4.3:88415340-88415359 | MS.gene000619:CDS | 30.0% | |
| AGTCTTAATAGCATCATCAA+GGG | - | chr4.3:88415133-88415152 | None:intergenic | 30.0% | |
| GTTTCAACAAAGGAATCAAT+GGG | + | chr4.3:88414808-88414827 | MS.gene000619:CDS | 30.0% | |
| TATTAAGACTACTAACCGTA+GGG | + | chr4.3:88415143-88415162 | MS.gene000619:CDS | 30.0% | |
| TGTTTCAACAAAGGAATCAA+TGG | + | chr4.3:88414807-88414826 | MS.gene000619:CDS | 30.0% | |
| TTCTTCATTTGCATTAACTG+AGG | + | chr4.3:88414846-88414865 | MS.gene000619:CDS | 30.0% | |
| ! | TAGTCTTAATAGCATCATCA+AGG | - | chr4.3:88415134-88415153 | None:intergenic | 30.0% |
| !! | TTTAGGTTGAAGAAGATTCA+GGG | + | chr4.3:88415258-88415277 | MS.gene000619:CDS | 30.0% |
| AAAGTTACAATTGCAGCAAG+GGG | + | chr4.3:88415341-88415360 | MS.gene000619:CDS | 35.0% | |
| AGAAGATTCAGGGTTATAAG+AGG | + | chr4.3:88415268-88415287 | MS.gene000619:CDS | 35.0% | |
| ATAAGAGGAGGGAAATTGAA+AGG | + | chr4.3:88415283-88415302 | MS.gene000619:CDS | 35.0% | |
| ATGTTAAAGAAGCTTCACTC+AGG | + | chr4.3:88414914-88414933 | MS.gene000619:CDS | 35.0% | |
| CTAATAGTATTCTCCAACCT+CGG | - | chr4.3:88415195-88415214 | None:intergenic | 35.0% | |
| CTATTAAGACTACTAACCGT+AGG | + | chr4.3:88415142-88415161 | MS.gene000619:CDS | 35.0% | |
| GAAAAATGATCTGACTGGAT+TGG | + | chr4.3:88415011-88415030 | MS.gene000619:CDS | 35.0% | |
| GAAAAGTTACAATTGCAGCA+AGG | + | chr4.3:88415339-88415358 | MS.gene000619:CDS | 35.0% | |
| TAATAGCATCATCAAGGGTA+AGG | - | chr4.3:88415128-88415147 | None:intergenic | 35.0% | |
| TAGTTACATTAAGGGTGAGT+TGG | + | chr4.3:88415212-88415231 | MS.gene000619:CDS | 35.0% | |
| TCAGCACTGTAATCAAACTT+TGG | - | chr4.3:88414979-88414998 | None:intergenic | 35.0% | |
| TGCTTATATTAAGGCGATTG+AGG | + | chr4.3:88415068-88415087 | MS.gene000619:CDS | 35.0% | |
| TGTTAAAGAAGCTTCACTCA+GGG | + | chr4.3:88414915-88414934 | MS.gene000619:CDS | 35.0% | |
| TTGAAAGGGAGGATTTCTTT+AGG | + | chr4.3:88415241-88415260 | MS.gene000619:CDS | 35.0% | |
| ! | AAGTTTGATTACAGTGCTGA+CGG | + | chr4.3:88414979-88414998 | MS.gene000619:CDS | 35.0% |
| !! | CTTTAGGTTGAAGAAGATTC+AGG | + | chr4.3:88415257-88415276 | MS.gene000619:CDS | 35.0% |
| !! | GTGTCGTGTTGCTTATATTA+AGG | + | chr4.3:88415059-88415078 | MS.gene000619:CDS | 35.0% |
| AAAGGAAGATGGTGATTGCT+AGG | + | chr4.3:88415301-88415320 | MS.gene000619:CDS | 40.0% | |
| AAGGAAGATGGTGATTGCTA+GGG | + | chr4.3:88415302-88415321 | MS.gene000619:CDS | 40.0% | |
| AGATTCAGGGTTATAAGAGG+AGG | + | chr4.3:88415271-88415290 | MS.gene000619:CDS | 40.0% | |
| ATCATCAAGGGTAAGGAAAG+AGG | - | chr4.3:88415121-88415140 | None:intergenic | 40.0% | |
| GAGGGAAATTGAAAGGAAGA+TGG | + | chr4.3:88415290-88415309 | MS.gene000619:CDS | 40.0% | |
| GATTCAGGGTTATAAGAGGA+GGG | + | chr4.3:88415272-88415291 | MS.gene000619:CDS | 40.0% | |
| GCAATCACAACGTTTGAATG+TGG | + | chr4.3:88414660-88414679 | MS.gene000619:CDS | 40.0% | |
| TGAAACCTCTCACAACTTGT+TGG | + | chr4.3:88415371-88415390 | MS.gene000619:CDS | 40.0% | |
| TTGAGAATGTTGTTAAGCCG+AGG | + | chr4.3:88415175-88415194 | MS.gene000619:CDS | 40.0% | |
| ! | TGAGTTGGATGAGCTTGAAA+GGG | + | chr4.3:88415227-88415246 | MS.gene000619:CDS | 40.0% |
| !! | TCCAGTCAGATCATTTTTCG+TGG | - | chr4.3:88415010-88415029 | None:intergenic | 40.0% |
| !! | TCTCAAGAGCATTAACCCTA+CGG | - | chr4.3:88415161-88415180 | None:intergenic | 40.0% |
| ACGTTCTCGTACTGAGAATG+TGG | + | chr4.3:88414939-88414958 | MS.gene000619:CDS | 45.0% | |
| ACTGAGGCTAAGTATGTAGC+CGG | + | chr4.3:88414862-88414881 | MS.gene000619:CDS | 45.0% | |
| GAATGTTGTTAAGCCGAGGT+TGG | + | chr4.3:88415179-88415198 | MS.gene000619:CDS | 45.0% | |
| GACAATGCTTGGAGTGGTTA+AGG | + | chr4.3:88414693-88414712 | MS.gene000619:CDS | 45.0% | |
| GACGTGCTTGATGTTATCAC+CGG | - | chr4.3:88414884-88414903 | None:intergenic | 45.0% | |
| GCCACGAAAAATGATCTGAC+TGG | + | chr4.3:88415006-88415025 | MS.gene000619:CDS | 45.0% | |
| ! | ACTCCAAGCATTGTCACTGT+GGG | - | chr4.3:88414688-88414707 | None:intergenic | 45.0% |
| ! | GTGAGTTGGATGAGCTTGAA+AGG | + | chr4.3:88415226-88415245 | MS.gene000619:CDS | 45.0% |
| ACCAGCCAACAAGTTGTGAG+AGG | - | chr4.3:88415379-88415398 | None:intergenic | 50.0% | |
| CACAGTGACAATGCTTGGAG+TGG | + | chr4.3:88414687-88414706 | MS.gene000619:CDS | 50.0% | |
| CGTTTGGTTGGAGCAACAAG+AGG | + | chr4.3:88414718-88414737 | MS.gene000619:CDS | 50.0% | |
| GTGGTTAAGGCACGTTTGGT+TGG | + | chr4.3:88414706-88414725 | MS.gene000619:CDS | 50.0% | |
| GTTGGATGAGCTTGAAAGGG+AGG | + | chr4.3:88415230-88415249 | MS.gene000619:CDS | 50.0% | |
| TCTCGTACTGAGAATGTGGC+AGG | + | chr4.3:88414943-88414962 | MS.gene000619:CDS | 50.0% | |
| TGGAGTGGTTAAGGCACGTT+TGG | + | chr4.3:88414702-88414721 | MS.gene000619:CDS | 50.0% | |
| TGGTACGACTGGAAAAGACG+AGG | + | chr4.3:88415395-88415414 | MS.gene000619:CDS | 50.0% | |
| ! | CACTCCAAGCATTGTCACTG+TGG | - | chr4.3:88414689-88414708 | None:intergenic | 50.0% |
| ! | CTCCAAGCATTGTCACTGTG+GGG | - | chr4.3:88414687-88414706 | None:intergenic | 50.0% |
| !! | AACTTGTTGGCTGGTACGAC+TGG | + | chr4.3:88415384-88415403 | MS.gene000619:CDS | 50.0% |
| !! | ACCTCTCACAACTTGTTGGC+TGG | + | chr4.3:88415375-88415394 | MS.gene000619:CDS | 50.0% |
| !! | GATCTGACTGGATTGGCTAG+AGG | + | chr4.3:88415018-88415037 | MS.gene000619:CDS | 50.0% |
| !! | TGATTACAGTGCTGACGGTG+AGG | + | chr4.3:88414984-88415003 | MS.gene000619:CDS | 50.0% |
| ATTGGCTAGAGGTGGTCAGC+AGG | + | chr4.3:88415029-88415048 | MS.gene000619:CDS | 55.0% | |
| GTCCCCACAGTGACAATGCT+TGG | + | chr4.3:88414682-88414701 | MS.gene000619:CDS | 55.0% | |
| !! | CTGACTGGATTGGCTAGAGG+TGG | + | chr4.3:88415021-88415040 | MS.gene000619:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 88414652 | 88415431 | 88414652 | ID=MS.gene000619 |
| chr4.3 | mRNA | 88414652 | 88415431 | 88414652 | ID=MS.gene000619.t1;Parent=MS.gene000619 |
| chr4.3 | exon | 88414652 | 88415431 | 88414652 | ID=MS.gene000619.t1.exon1;Parent=MS.gene000619.t1 |
| chr4.3 | CDS | 88414652 | 88415431 | 88414652 | ID=cds.MS.gene000619.t1;Parent=MS.gene000619.t1 |
| Gene Sequence |
| Protein sequence |
