Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28505.t1 | XP_024628545.1 | 98 | 452 | 4 | 1 | 1 | 447 | 1 | 452 | 1.10E-247 | 865.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28505.t1 | Q9LX65 | 77.2 | 451 | 87 | 2 | 1 | 446 | 1 | 440 | 9.5e-197 | 687.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28505.t1 | A0A396GM22 | 97.6 | 453 | 5 | 1 | 1 | 447 | 1 | 453 | 6.4e-247 | 862.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene024661 | MS.gene28505 | PPI |
| MS.gene000619 | MS.gene28505 | PPI |
| MS.gene28505 | MS.gene22330 | PPI |
| MS.gene34307 | MS.gene28505 | PPI |
| MS.gene55771 | MS.gene28505 | PPI |
| MS.gene28505 | MS.gene06042 | PPI |
| MS.gene28505 | MS.gene034241 | PPI |
| MS.gene072752 | MS.gene28505 | PPI |
| MS.gene28505 | MS.gene98140 | PPI |
| MS.gene048900 | MS.gene28505 | PPI |
| MS.gene91075 | MS.gene28505 | PPI |
| MS.gene28505 | MS.gene053216 | PPI |
| MS.gene28505 | MS.gene034283 | PPI |
| MS.gene28505 | MS.gene21642 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28505.t1 | MTR_8g078370 | 90.947 | 486 | 5 | 1 | 1 | 447 | 1 | 486 | 0.0 | 889 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28505.t1 | AT3G42050 | 77.162 | 451 | 87 | 2 | 1 | 446 | 1 | 440 | 0.0 | 719 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGGTCCTATTAACAATTT+TGG | 0.135845 | 8.4:+28286070 | MS.gene28505:CDS |
| TGAGCCTGCAATTGAATATT+TGG | 0.152768 | 8.4:+28284319 | MS.gene28505:CDS |
| TCACCCGTTCCTTGGCTTTA+AGG | 0.180102 | 8.4:-28286187 | None:intergenic |
| GAAATCATGCTCTTCAAAAT+TGG | 0.212649 | 8.4:-28285620 | None:intergenic |
| TTCATGTGTTGCAGATTCTA+AGG | 0.279693 | 8.4:+28286051 | MS.gene28505:intron |
| ACGAGTCCAAAATTGTTAAT+AGG | 0.288657 | 8.4:-28286076 | None:intergenic |
| ACATCATCAATACTGGTAAA+TGG | 0.305918 | 8.4:-28282807 | None:intergenic |
| ATGAAACATGTCTCTGCATT+TGG | 0.326157 | 8.4:+28284282 | MS.gene28505:CDS |
| AGATCCTTGCCTTAATAGTT+AGG | 0.340589 | 8.4:+28282608 | MS.gene28505:CDS |
| AGATACTGTGGAGTATGTTT+TGG | 0.345588 | 8.4:+28279630 | MS.gene28505:CDS |
| AACCTGATCGGTGGTTAATT+CGG | 0.348733 | 8.4:-28279148 | None:intergenic |
| TGCACAAAGATCCCATCTTT+TGG | 0.361790 | 8.4:+28285584 | MS.gene28505:CDS |
| AGAAGTTCTTCTCGGCAATC+TGG | 0.364797 | 8.4:+28285549 | MS.gene28505:CDS |
| TTCATCCAGTGCCATCCGGC+TGG | 0.367544 | 8.4:+28286146 | MS.gene28505:CDS |
| GACTGCTTCGGAAAGGAAAC+TGG | 0.368536 | 8.4:+28282563 | MS.gene28505:intron |
| AAAGTATGCAAGCTTCTTGC+AGG | 0.371219 | 8.4:+28286286 | MS.gene28505:CDS |
| TTGAATATTTGGCTACTTCA+AGG | 0.384243 | 8.4:+28284330 | MS.gene28505:CDS |
| TTTGTATTACCTAACTATTA+AGG | 0.390901 | 8.4:-28282617 | None:intergenic |
| AATCAAAACATCATCAATAC+TGG | 0.405259 | 8.4:-28282814 | None:intergenic |
| AGCTAGAGTCCTTGGATCAT+TGG | 0.405799 | 8.4:-28286099 | None:intergenic |
| GTAGCCAAATATTCAATTGC+AGG | 0.408085 | 8.4:-28284323 | None:intergenic |
| GGTAAAATGGCTTTGTGAGC+AGG | 0.413777 | 8.4:+28282842 | MS.gene28505:CDS |
| CTTTGGCAGCGAACCCGAAA+AGG | 0.417733 | 8.4:+28279764 | MS.gene28505:intron |
| CTCTCTGGAAGAAGGGCTTA+AGG | 0.434534 | 8.4:+28285486 | MS.gene28505:CDS |
| TTCTTCTCGGCAATCTGGAT+TGG | 0.439850 | 8.4:+28285554 | MS.gene28505:CDS |
| CATCAAGTAGCTGTGCTCTC+TGG | 0.442970 | 8.4:-28279425 | None:intergenic |
| ATGTCCACCAAGCTCATCTC+TGG | 0.443004 | 8.4:+28279357 | MS.gene28505:CDS |
| TCCGCCCAGCCGGATGGCAC+TGG | 0.445744 | 8.4:-28286151 | None:intergenic |
| TACAAGCAAGAAGTTCTTCT+CGG | 0.448235 | 8.4:+28285541 | MS.gene28505:CDS |
| CAAGCAACAGCTAGAGTCCT+TGG | 0.449302 | 8.4:-28286107 | None:intergenic |
| GTTGGAGGCACTGAACTCTC+TGG | 0.462163 | 8.4:+28285471 | MS.gene28505:CDS |
| TTGATGTCAAAAGGGACATT+GGG | 0.464953 | 8.4:+28284614 | MS.gene28505:CDS |
| CTTGATGTCAAAAGGGACAT+TGG | 0.466332 | 8.4:+28284613 | MS.gene28505:CDS |
| ATCCACTCAACAATCTATTC+AGG | 0.466562 | 8.4:+28283984 | MS.gene28505:CDS |
| TCGTACTTTGTTCAAACTGA+TGG | 0.468044 | 8.4:+28283928 | MS.gene28505:CDS |
| GAAAGGCAATTGATAGCAGT+TGG | 0.470551 | 8.4:-28283877 | None:intergenic |
| TAGTTTCATCACCCGTTCCT+TGG | 0.471725 | 8.4:-28286195 | None:intergenic |
| GATTGTGTGGCAGGATGGTC+CGG | 0.472350 | 8.4:+28279561 | MS.gene28505:intron |
| TAAGAGTTCTACAAAAGAAA+AGG | 0.474052 | 8.4:+28284379 | MS.gene28505:CDS |
| TACTGAAGGAACCTGTGGTC+AGG | 0.482323 | 8.4:+28283905 | MS.gene28505:CDS |
| GTGGACATGTAGGTTTCCCA+TGG | 0.486337 | 8.4:-28279342 | None:intergenic |
| AGGGACATTGGGTGCTCAGA+TGG | 0.492699 | 8.4:+28284625 | MS.gene28505:CDS |
| TTAACAACATCAATCAGTCT+TGG | 0.496128 | 8.4:-28284359 | None:intergenic |
| GATGAGCTTGGTGGACATGT+AGG | 0.497249 | 8.4:-28279352 | None:intergenic |
| TCATGTGTTGCAGATTCTAA+GGG | 0.497992 | 8.4:+28286052 | MS.gene28505:intron |
| CGTAACGGCGTAAGAGTTGA+AGG | 0.499260 | 8.4:-28279386 | None:intergenic |
| CGTACTTTGTTCAAACTGAT+GGG | 0.502271 | 8.4:+28283929 | MS.gene28505:CDS |
| TGACCTTAAAGCCAAGGAAC+GGG | 0.504993 | 8.4:+28286184 | MS.gene28505:CDS |
| GCACTGAACTCTCTGGAAGA+AGG | 0.505091 | 8.4:+28285478 | MS.gene28505:CDS |
| TCATCCAGTGCCATCCGGCT+GGG | 0.508926 | 8.4:+28286147 | MS.gene28505:CDS |
| ACAGTTCATCCAGTGCCATC+CGG | 0.510383 | 8.4:+28286142 | MS.gene28505:CDS |
| GTCACAATGATCCGCCCAGC+CGG | 0.514567 | 8.4:-28286161 | None:intergenic |
| TTCAAACTTTGAACAACTTG+TGG | 0.515858 | 8.4:-28284662 | None:intergenic |
| AGCCGAATTAACCACCGATC+AGG | 0.518255 | 8.4:+28279146 | MS.gene28505:CDS |
| TTTGGCAGCGAACCCGAAAA+GGG | 0.526452 | 8.4:+28279765 | MS.gene28505:intron |
| CAGAGTGGCGTTGCTTCAAA+CGG | 0.528770 | 8.4:+28282762 | MS.gene28505:CDS |
| GCAGTTGGAACTCCACGACT+TGG | 0.537420 | 8.4:-28283862 | None:intergenic |
| GATCGGTGGTTAATTCGGCT+TGG | 0.538244 | 8.4:-28279143 | None:intergenic |
| AAAGTTTGAAAGCACAAGCA+TGG | 0.539296 | 8.4:+28284675 | MS.gene28505:CDS |
| CAACATCAATCAGTCTTGGA+AGG | 0.542110 | 8.4:-28284355 | None:intergenic |
| CACTGAACTCTCTGGAAGAA+GGG | 0.545338 | 8.4:+28285479 | MS.gene28505:CDS |
| TTGCCTTTCAACTCTACTGA+AGG | 0.554620 | 8.4:+28283891 | MS.gene28505:CDS |
| TTCTGAGCAGGGATATCCCA+TGG | 0.555770 | 8.4:+28279325 | MS.gene28505:CDS |
| TGTTCCAGAGATGAGCTTGG+TGG | 0.555799 | 8.4:-28279361 | None:intergenic |
| ATAGATTGTTGAGTGGATGC+TGG | 0.556132 | 8.4:-28283979 | None:intergenic |
| GTTTCATGACAATGCTCTTG+CGG | 0.557830 | 8.4:+28279795 | MS.gene28505:CDS |
| CAATGATCCGCCCAGCCGGA+TGG | 0.559951 | 8.4:-28286157 | None:intergenic |
| ACAAACACGCGAACATAAGC+CGG | 0.563310 | 8.4:-28279580 | None:intergenic |
| AAAGATGGGATCTTTGTGCA+TGG | 0.563821 | 8.4:-28285581 | None:intergenic |
| CGGATCGGAACGAACCTGAT+CGG | 0.567280 | 8.4:-28279160 | None:intergenic |
| TTTCTGGAAGGTTCTGAGCA+GGG | 0.571661 | 8.4:+28279314 | MS.gene28505:intron |
| ATGATAGATGAAATGCTGAC+AGG | 0.587203 | 8.4:+28279655 | MS.gene28505:CDS |
| GTAAATACTTAACTTAGGAA+AGG | 0.587250 | 8.4:-28279835 | None:intergenic |
| TTGCACCCAAGAAAAGTCGT+TGG | 0.588611 | 8.4:-28286265 | None:intergenic |
| TGGACTCGTCCAATGATCCA+AGG | 0.596481 | 8.4:+28286090 | MS.gene28505:CDS |
| ACTCCACGACTTGGATGAGA+AGG | 0.596749 | 8.4:-28283853 | None:intergenic |
| GCTTGTTCCAGAGATGAGCT+TGG | 0.598187 | 8.4:-28279364 | None:intergenic |
| AACATCAATCAGTCTTGGAA+GGG | 0.598970 | 8.4:-28284354 | None:intergenic |
| AAGATGGGATCTTTGTGCAT+GGG | 0.601062 | 8.4:-28285580 | None:intergenic |
| GTTCCTTCAGTAGAGTTGAA+AGG | 0.604480 | 8.4:-28283894 | None:intergenic |
| GTGGATGCTGGACAAATCAA+TGG | 0.605600 | 8.4:-28283967 | None:intergenic |
| AAACCTTCTCATCCAAGTCG+TGG | 0.605881 | 8.4:+28283850 | MS.gene28505:CDS |
| ATTACCTAACTATTAAGGCA+AGG | 0.608709 | 8.4:-28282612 | None:intergenic |
| AACTCTACTGAAGGAACCTG+TGG | 0.612048 | 8.4:+28283900 | MS.gene28505:CDS |
| TCCAGTGCCATCCGGCTGGG+CGG | 0.612825 | 8.4:+28286150 | MS.gene28505:CDS |
| TGGACATGTAGGTTTCCCAT+GGG | 0.621215 | 8.4:-28279341 | None:intergenic |
| CTGACCTTAAAGCCAAGGAA+CGG | 0.626093 | 8.4:+28286183 | MS.gene28505:CDS |
| ACAAAGTACGACCTGACCAC+AGG | 0.637592 | 8.4:-28283916 | None:intergenic |
| TGTGACTGACCTTAAAGCCA+AGG | 0.638812 | 8.4:+28286178 | MS.gene28505:CDS |
| GTACTTTGTTCAAACTGATG+GGG | 0.648822 | 8.4:+28283930 | MS.gene28505:CDS |
| TCTGAGCAGGGATATCCCAT+GGG | 0.668404 | 8.4:+28279326 | MS.gene28505:CDS |
| ATCGGAACGAACCTGATCGG+TGG | 0.686459 | 8.4:-28279157 | None:intergenic |
| TTTCTGATCGATGATCGTAA+CGG | 0.689054 | 8.4:-28279401 | None:intergenic |
| AATGAATCATGAGAGTGCCG+AGG | 0.694038 | 8.4:+28286217 | MS.gene28505:CDS |
| TACCTGAATAGATTGTTGAG+TGG | 0.694123 | 8.4:-28283986 | None:intergenic |
| AGCACAAGCATGGAGTGATG+AGG | 0.723682 | 8.4:+28284685 | MS.gene28505:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 28279138 | 28286313 | 28279138 | ID=MS.gene28505 |
| chr8.4 | mRNA | 28279138 | 28286313 | 28279138 | ID=MS.gene28505.t1;Parent=MS.gene28505 |
| chr8.4 | exon | 28279138 | 28279167 | 28279138 | ID=MS.gene28505.t1.exon1;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28279138 | 28279167 | 28279138 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28279324 | 28279449 | 28279324 | ID=MS.gene28505.t1.exon2;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28279324 | 28279449 | 28279324 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28279574 | 28279676 | 28279574 | ID=MS.gene28505.t1.exon3;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28279574 | 28279676 | 28279574 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28279773 | 28279844 | 28279773 | ID=MS.gene28505.t1.exon4;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28279773 | 28279844 | 28279773 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28282578 | 28282629 | 28282578 | ID=MS.gene28505.t1.exon5;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28282578 | 28282629 | 28282578 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28282746 | 28282863 | 28282746 | ID=MS.gene28505.t1.exon6;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28282746 | 28282863 | 28282746 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28283844 | 28284005 | 28283844 | ID=MS.gene28505.t1.exon7;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28283844 | 28284005 | 28283844 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28284275 | 28284400 | 28284275 | ID=MS.gene28505.t1.exon8;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28284275 | 28284400 | 28284275 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28284581 | 28284706 | 28284581 | ID=MS.gene28505.t1.exon9;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28284581 | 28284706 | 28284581 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28285466 | 28285645 | 28285466 | ID=MS.gene28505.t1.exon10;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28285466 | 28285645 | 28285466 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| chr8.4 | exon | 28286065 | 28286313 | 28286065 | ID=MS.gene28505.t1.exon11;Parent=MS.gene28505.t1 |
| chr8.4 | CDS | 28286065 | 28286313 | 28286065 | ID=cds.MS.gene28505.t1;Parent=MS.gene28505.t1 |
| Gene Sequence |
| Protein sequence |
