Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000938.t1 | XP_013446994.1 | 100 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 5.80E-28 | 132.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000938.t1 | A0A072TV19 | 100.0 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 4.2e-28 | 132.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene90025 | MS.gene000938 | PPI |
| MS.gene98091 | MS.gene000938 | PPI |
| MS.gene000435 | MS.gene000938 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000938.t1 | MTR_8g096840 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 3.69e-43 | 134 |
| MS.gene000938.t1 | MTR_3g085020 | 84.058 | 69 | 11 | 0 | 1 | 69 | 1 | 69 | 2.04e-37 | 119 |
| MS.gene000938.t1 | MTR_7g028975 | 66.667 | 69 | 23 | 0 | 1 | 69 | 1 | 69 | 4.18e-33 | 108 |
| MS.gene000938.t1 | MTR_8g097020 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 9.24e-32 | 105 |
| MS.gene000938.t1 | MTR_8g027820 | 62.319 | 69 | 26 | 0 | 1 | 69 | 1 | 69 | 4.12e-30 | 102 |
| MS.gene000938.t1 | MTR_7g028870 | 69.697 | 66 | 20 | 0 | 2 | 67 | 3 | 68 | 4.73e-30 | 100 |
| MS.gene000938.t1 | MTR_3g085030 | 59.420 | 69 | 28 | 0 | 1 | 69 | 1 | 69 | 1.41e-28 | 97.1 |
| MS.gene000938.t1 | MTR_7g028940 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 2.74e-26 | 91.3 |
| MS.gene000938.t1 | MTR_8g097030 | 72.059 | 68 | 19 | 0 | 2 | 69 | 3 | 70 | 4.68e-25 | 88.2 |
| MS.gene000938.t1 | MTR_7g028815 | 60.000 | 55 | 22 | 0 | 1 | 55 | 1 | 55 | 4.37e-22 | 82.0 |
| MS.gene000938.t1 | MTR_7g028945 | 44.776 | 67 | 37 | 0 | 1 | 67 | 1 | 67 | 1.32e-16 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000938.t1 | AT3G48660 | 79.710 | 69 | 14 | 0 | 1 | 69 | 1 | 69 | 1.77e-36 | 117 |
| MS.gene000938.t1 | AT5G63500 | 81.159 | 69 | 13 | 0 | 1 | 69 | 1 | 69 | 7.26e-36 | 115 |
| MS.gene000938.t1 | AT5G08391 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 1.28e-29 | 99.8 |
| MS.gene000938.t1 | AT3G27027 | 68.182 | 66 | 21 | 0 | 2 | 67 | 3 | 68 | 2.10e-29 | 99.4 |
| MS.gene000938.t1 | AT3G27030 | 65.152 | 66 | 23 | 0 | 1 | 66 | 1 | 66 | 3.48e-27 | 95.9 |
| MS.gene000938.t1 | AT3G27030 | 55.072 | 69 | 31 | 0 | 1 | 69 | 74 | 142 | 1.05e-24 | 89.7 |
| MS.gene000938.t1 | AT5G40980 | 76.364 | 55 | 13 | 0 | 2 | 56 | 3 | 57 | 1.87e-26 | 92.0 |
| MS.gene000938.t1 | AT3G01950 | 55.224 | 67 | 30 | 0 | 3 | 69 | 4 | 70 | 3.09e-25 | 89.0 |
| MS.gene000938.t1 | AT5G14110 | 55.385 | 65 | 29 | 0 | 3 | 67 | 4 | 68 | 1.79e-24 | 87.0 |
| MS.gene000938.t1 | AT3G01940 | 69.697 | 66 | 20 | 0 | 2 | 67 | 4 | 69 | 1.52e-22 | 82.0 |
| MS.gene000938.t1 | AT5G50560 | 56.452 | 62 | 27 | 0 | 1 | 62 | 1 | 62 | 1.96e-17 | 68.9 |
| MS.gene000938.t1 | AT5G50660 | 56.452 | 62 | 27 | 0 | 1 | 62 | 1 | 62 | 1.96e-17 | 68.9 |
| MS.gene000938.t1 | AT5G40960 | 43.284 | 67 | 38 | 0 | 1 | 67 | 1 | 67 | 1.12e-13 | 59.7 |
Find 0 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTATTTTACTCATTGCTGT+TGG | + | 57534:9565-9584 | MS.gene000938:CDS | 30.0% |
| AATGATGGCATGAACAAGAA+TGG | - | 57534:9530-9549 | None:intergenic | 35.0% | |
| GATGAGACCAAAGTAAATGA+TGG | - | 57534:9545-9564 | None:intergenic | 35.0% | |
| GTTCATGCCATCATTTACTT+TGG | + | 57534:9535-9554 | MS.gene000938:CDS | 35.0% | |
| GAGGAAAGGTGATTGAGTTT+GGG | + | 57534:9482-9501 | MS.gene000938:CDS | 40.0% | |
| TTGTTTCAAATACCTGGTCG+AGG | + | 57534:9463-9482 | MS.gene000938:CDS | 40.0% | |
| TTTGGGAACATGCAAACAAG+TGG | + | 57534:9499-9518 | MS.gene000938:CDS | 40.0% | |
| ! | GGTATTTGAAACAACAGACC+TGG | - | 57534:9457-9476 | None:intergenic | 40.0% |
| ! | TTGGTGTTCACATCTACACT+GGG | + | 57534:9584-9603 | MS.gene000938:CDS | 40.0% |
| !! | GGTCTGTTGTTTCAAATACC+TGG | + | 57534:9457-9476 | MS.gene000938:CDS | 40.0% |
| CGAGGAAAGGTGATTGAGTT+TGG | + | 57534:9481-9500 | MS.gene000938:CDS | 45.0% | |
| TCAAATACCTGGTCGAGGAA+AGG | + | 57534:9468-9487 | MS.gene000938:CDS | 45.0% | |
| ! | GTTGGTGTTCACATCTACAC+TGG | + | 57534:9583-9602 | MS.gene000938:CDS | 45.0% |
| TCAATCACCTTTCCTCGACC+AGG | - | 57534:9478-9497 | None:intergenic | 50.0% | |
| ! | CTGTTTGTGCTGTTGAGTCC+AGG | + | 57534:9436-9455 | MS.gene000938:CDS | 50.0% |
| AGCACCACCGCTATGATCAC+CGG | - | 57534:9418-9437 | None:intergenic | 55.0% | |
| TTGGGGTCCGGTGATCATAG+CGG | + | 57534:9408-9427 | MS.gene000938:CDS | 55.0% | |
| GGGTCCGGTGATCATAGCGG+TGG | + | 57534:9411-9430 | MS.gene000938:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57534 | gene | 9400 | 9609 | 9400 | ID=MS.gene000938 |
| 57534 | mRNA | 9400 | 9609 | 9400 | ID=MS.gene000938.t1;Parent=MS.gene000938 |
| 57534 | exon | 9400 | 9609 | 9400 | ID=MS.gene000938.t1.exon1;Parent=MS.gene000938.t1 |
| 57534 | CDS | 9400 | 9609 | 9400 | ID=cds.MS.gene000938.t1;Parent=MS.gene000938.t1 |
| Gene Sequence |
| Protein sequence |
