Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000435.t1 | AFK41617.1 | 97.1 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 8.90E-29 | 135.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000435.t1 | I3SMX5 | 97.1 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 6.4e-29 | 135.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75628 | MS.gene000435 | PPI |
| MS.gene030447 | MS.gene000435 | PPI |
| MS.gene72191 | MS.gene000435 | PPI |
| MS.gene000435 | MS.gene77577 | PPI |
| MS.gene045301 | MS.gene000435 | PPI |
| MS.gene000435 | MS.gene000938 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000435.t1 | MTR_7g028940 | 97.101 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 4.86e-43 | 133 |
| MS.gene000435.t1 | MTR_8g096840 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 4.91e-26 | 90.9 |
| MS.gene000435.t1 | MTR_3g085020 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 2.82e-24 | 86.3 |
| MS.gene000435.t1 | MTR_8g097020 | 49.275 | 69 | 35 | 0 | 1 | 69 | 1 | 69 | 6.64e-22 | 80.5 |
| MS.gene000435.t1 | MTR_8g027820 | 49.275 | 69 | 35 | 0 | 1 | 69 | 1 | 69 | 2.89e-21 | 79.7 |
| MS.gene000435.t1 | MTR_7g028975 | 47.826 | 69 | 36 | 0 | 1 | 69 | 1 | 69 | 6.64e-21 | 77.8 |
| MS.gene000435.t1 | MTR_7g028870 | 42.424 | 66 | 38 | 0 | 2 | 67 | 3 | 68 | 1.03e-18 | 72.4 |
| MS.gene000435.t1 | MTR_3g085030 | 44.928 | 69 | 38 | 0 | 1 | 69 | 1 | 69 | 1.08e-18 | 72.4 |
| MS.gene000435.t1 | MTR_8g097030 | 57.353 | 68 | 29 | 0 | 2 | 69 | 3 | 70 | 2.59e-17 | 68.9 |
| MS.gene000435.t1 | MTR_7g028815 | 54.717 | 53 | 24 | 0 | 1 | 53 | 1 | 53 | 4.36e-17 | 69.3 |
| MS.gene000435.t1 | MTR_7g028945 | 40.299 | 67 | 40 | 0 | 1 | 67 | 1 | 67 | 1.44e-12 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000435.t1 | AT3G27030 | 68.116 | 69 | 22 | 0 | 1 | 69 | 74 | 142 | 1.80e-31 | 106 |
| MS.gene000435.t1 | AT3G27030 | 50.000 | 66 | 33 | 0 | 1 | 66 | 1 | 66 | 4.01e-19 | 75.5 |
| MS.gene000435.t1 | AT3G48660 | 55.072 | 69 | 31 | 0 | 1 | 69 | 1 | 69 | 4.11e-25 | 89.0 |
| MS.gene000435.t1 | AT5G63500 | 53.623 | 69 | 32 | 0 | 1 | 69 | 1 | 69 | 7.58e-24 | 85.1 |
| MS.gene000435.t1 | AT5G08391 | 50.725 | 69 | 34 | 0 | 1 | 69 | 1 | 69 | 4.74e-22 | 80.9 |
| MS.gene000435.t1 | AT5G14110 | 55.224 | 67 | 30 | 0 | 3 | 69 | 4 | 70 | 2.09e-21 | 79.3 |
| MS.gene000435.t1 | AT3G01950 | 49.254 | 67 | 34 | 0 | 3 | 69 | 4 | 70 | 3.82e-21 | 78.6 |
| MS.gene000435.t1 | AT3G27027 | 42.424 | 66 | 38 | 0 | 2 | 67 | 3 | 68 | 7.68e-19 | 72.8 |
| MS.gene000435.t1 | AT5G40980 | 45.283 | 53 | 29 | 0 | 2 | 54 | 3 | 55 | 4.82e-15 | 63.2 |
| MS.gene000435.t1 | AT3G01940 | 43.939 | 66 | 37 | 0 | 2 | 67 | 4 | 69 | 9.65e-13 | 57.4 |
| MS.gene000435.t1 | AT5G50560 | 47.619 | 63 | 33 | 0 | 1 | 63 | 1 | 63 | 9.13e-12 | 54.7 |
| MS.gene000435.t1 | AT5G50660 | 47.619 | 63 | 33 | 0 | 1 | 63 | 1 | 63 | 9.13e-12 | 54.7 |
| MS.gene000435.t1 | AT5G40960 | 40.299 | 67 | 40 | 0 | 1 | 67 | 1 | 67 | 1.05e-11 | 54.7 |
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTGGGCACCAGTGGTTAT+TGG | 0.332087 | 7.1:+68592774 | MS.gene000435:CDS |
| TCTCTATGCTGTTATCATCT+TGG | 0.333421 | 7.1:+68592926 | MS.gene000435:CDS |
| TTGTTTGCATTTCCAGGAAA+TGG | 0.335454 | 7.1:+68592831 | MS.gene000435:CDS |
| CTTCATGTTCATATCTATAC+TGG | 0.339150 | 7.1:+68592951 | MS.gene000435:CDS |
| GGGTTGTTGTTTGCATTTCC+AGG | 0.340837 | 7.1:+68592825 | MS.gene000435:CDS |
| AATGGAAAACAGCTTGAGTT+TGG | 0.402355 | 7.1:+68592849 | MS.gene000435:CDS |
| CTGTTTGTGTTTCTTCAACC+AGG | 0.411752 | 7.1:+68592804 | MS.gene000435:CDS |
| TTTGGTGGTATGAAGACAAA+TGG | 0.496601 | 7.1:+68592867 | MS.gene000435:CDS |
| GGAAAACAGCTTGAGTTTGG+TGG | 0.507655 | 7.1:+68592852 | MS.gene000435:CDS |
| TGTTTGTGTTTCTTCAACCA+GGG | 0.532155 | 7.1:+68592805 | MS.gene000435:CDS |
| AATGTCTGATTGGGCACCAG+TGG | 0.594590 | 7.1:+68592767 | None:intergenic |
| AGCACAACTCCAATAACCAC+TGG | 0.602053 | 7.1:-68592783 | None:intergenic |
| GGAAATGCAAACAACAACCC+TGG | 0.647896 | 7.1:-68592822 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCATACACTTATTTTCTT+TGG | + | chr7.1:68592903-68592922 | MS.gene000435:CDS | 20.0% |
| CTTCATGTTCATATCTATAC+TGG | + | chr7.1:68592951-68592970 | MS.gene000435:CDS | 30.0% | |
| AATGGAAAACAGCTTGAGTT+TGG | + | chr7.1:68592849-68592868 | MS.gene000435:CDS | 35.0% | |
| TCTCTATGCTGTTATCATCT+TGG | + | chr7.1:68592926-68592945 | MS.gene000435:CDS | 35.0% | |
| TGTTTGTGTTTCTTCAACCA+GGG | + | chr7.1:68592805-68592824 | MS.gene000435:CDS | 35.0% | |
| TTGTTTGCATTTCCAGGAAA+TGG | + | chr7.1:68592831-68592850 | MS.gene000435:CDS | 35.0% | |
| ! | TTTGGTGGTATGAAGACAAA+TGG | + | chr7.1:68592867-68592886 | MS.gene000435:CDS | 35.0% |
| CTGTTTGTGTTTCTTCAACC+AGG | + | chr7.1:68592804-68592823 | MS.gene000435:CDS | 40.0% | |
| !! | TCAAGCTGTTTTCCATTTCC+TGG | - | chr7.1:68592846-68592865 | None:intergenic | 40.0% |
| AGCACAACTCCAATAACCAC+TGG | - | chr7.1:68592786-68592805 | None:intergenic | 45.0% | |
| GGAAAACAGCTTGAGTTTGG+TGG | + | chr7.1:68592852-68592871 | MS.gene000435:CDS | 45.0% | |
| GGAAATGCAAACAACAACCC+TGG | - | chr7.1:68592825-68592844 | None:intergenic | 45.0% | |
| !! | GGGTTGTTGTTTGCATTTCC+AGG | + | chr7.1:68592825-68592844 | MS.gene000435:CDS | 45.0% |
| GATTGGGCACCAGTGGTTAT+TGG | + | chr7.1:68592774-68592793 | MS.gene000435:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 68592768 | 68592977 | 68592768 | ID=MS.gene000435 |
| chr7.1 | mRNA | 68592768 | 68592977 | 68592768 | ID=MS.gene000435.t1;Parent=MS.gene000435 |
| chr7.1 | exon | 68592768 | 68592977 | 68592768 | ID=MS.gene000435.t1.exon1;Parent=MS.gene000435.t1 |
| chr7.1 | CDS | 68592768 | 68592977 | 68592768 | ID=cds.MS.gene000435.t1;Parent=MS.gene000435.t1 |
| Gene Sequence |
| Protein sequence |
