Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98091.t1 | AFK41617.1 | 97.1 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 8.90E-29 | 135.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98091.t1 | I3SMX5 | 97.1 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 6.4e-29 | 135.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene98091 | 0.810263 | 1.26E-50 | -1.69E-46 |
| MS.gene049159 | MS.gene98091 | 0.849937 | 2.35E-60 | -1.69E-46 |
| MS.gene049254 | MS.gene98091 | 0.82831 | 9.65E-55 | -1.69E-46 |
| MS.gene049404 | MS.gene98091 | 0.821329 | 4.28E-53 | -1.69E-46 |
| MS.gene049435 | MS.gene98091 | 0.807938 | 3.96E-50 | -1.69E-46 |
| MS.gene049501 | MS.gene98091 | 0.812309 | 4.52E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene98091 | 0.815086 | 1.11E-51 | -1.69E-46 |
| MS.gene049821 | MS.gene98091 | 0.801789 | 7.63E-49 | -1.69E-46 |
| MS.gene049850 | MS.gene98091 | 0.824325 | 8.58E-54 | -1.69E-46 |
| MS.gene049983 | MS.gene98091 | 0.803135 | 4.03E-49 | -1.69E-46 |
| MS.gene050237 | MS.gene98091 | 0.81701 | 4.12E-52 | -1.69E-46 |
| MS.gene050273 | MS.gene98091 | 0.81766 | 2.94E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene98091 | 0.800384 | 1.48E-48 | -1.69E-46 |
| MS.gene050759 | MS.gene98091 | 0.827689 | 1.36E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene98091 | 0.821692 | 3.53E-53 | -1.69E-46 |
| MS.gene050826 | MS.gene98091 | 0.812317 | 4.51E-51 | -1.69E-46 |
| MS.gene050962 | MS.gene98091 | 0.820475 | 6.73E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene98091 | 0.838595 | 2.62E-57 | -1.69E-46 |
| MS.gene051247 | MS.gene98091 | 0.808069 | 3.71E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene98091 | 0.824716 | 6.94E-54 | -1.69E-46 |
| MS.gene051719 | MS.gene98091 | 0.819844 | 9.38E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene98091 | 0.830347 | 3.09E-55 | -1.69E-46 |
| MS.gene051744 | MS.gene98091 | 0.813068 | 3.09E-51 | -1.69E-46 |
| MS.gene051800 | MS.gene98091 | 0.820144 | 8.01E-53 | -1.69E-46 |
| MS.gene052037 | MS.gene98091 | 0.803181 | 3.94E-49 | -1.69E-46 |
| MS.gene05215 | MS.gene98091 | 0.815173 | 1.06E-51 | -1.69E-46 |
| MS.gene052323 | MS.gene98091 | 0.808523 | 2.97E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene98091 | 0.852927 | 3.35E-61 | -1.69E-46 |
| MS.gene052519 | MS.gene98091 | 0.823943 | 1.06E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene98091 | 0.815041 | 1.13E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene98091 | 0.822097 | 2.84E-53 | -1.69E-46 |
| MS.gene052611 | MS.gene98091 | 0.834345 | 3.16E-56 | -1.69E-46 |
| MS.gene052665 | MS.gene98091 | 0.815009 | 1.15E-51 | -1.69E-46 |
| MS.gene052773 | MS.gene98091 | 0.828363 | 9.37E-55 | -1.69E-46 |
| MS.gene052863 | MS.gene98091 | 0.847436 | 1.16E-59 | -1.69E-46 |
| MS.gene052896 | MS.gene98091 | 0.838519 | 2.74E-57 | -1.69E-46 |
| MS.gene053008 | MS.gene98091 | 0.829037 | 6.44E-55 | -1.69E-46 |
| MS.gene053079 | MS.gene98091 | 0.808176 | 3.52E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene98091 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene98091 | 0.819706 | 1.01E-52 | -1.69E-46 |
| MS.gene05350 | MS.gene98091 | 0.800018 | 1.75E-48 | -1.69E-46 |
| MS.gene053894 | MS.gene98091 | 0.804001 | 2.66E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene98091 | 0.8206 | 6.30E-53 | -1.69E-46 |
| MS.gene054236 | MS.gene98091 | 0.810683 | 1.02E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene98091 | 0.824598 | 7.40E-54 | -1.69E-46 |
| MS.gene054291 | MS.gene98091 | -0.812808 | 3.52E-51 | -1.69E-46 |
| MS.gene054467 | MS.gene98091 | 0.817642 | 2.97E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene98091 | 0.803063 | 4.17E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene98091 | 0.822496 | 2.30E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene98091 | 0.802157 | 6.41E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene98091 | 0.835429 | 1.69E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene98091 | 0.820025 | 8.53E-53 | -1.69E-46 |
| MS.gene055144 | MS.gene98091 | 0.804133 | 2.50E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene98091 | 0.807523 | 4.85E-50 | -1.69E-46 |
| MS.gene055227 | MS.gene98091 | 0.83593 | 1.26E-56 | -1.69E-46 |
| MS.gene055228 | MS.gene98091 | 0.817409 | 3.35E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene98091 | 0.808426 | 3.12E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene98091 | 0.821912 | 3.14E-53 | -1.69E-46 |
| MS.gene055512 | MS.gene98091 | 0.828587 | 8.28E-55 | -1.69E-46 |
| MS.gene055623 | MS.gene98091 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene98091 | 0.833489 | 5.18E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene98091 | 0.849125 | 3.95E-60 | -1.69E-46 |
| MS.gene05604 | MS.gene98091 | 0.820742 | 5.84E-53 | -1.69E-46 |
| MS.gene056173 | MS.gene98091 | 0.803271 | 3.78E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene98091 | 0.826211 | 3.07E-54 | -1.69E-46 |
| MS.gene056308 | MS.gene98091 | 0.807536 | 4.82E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene98091 | 0.812113 | 4.99E-51 | -1.69E-46 |
| MS.gene056507 | MS.gene98091 | 0.813157 | 2.95E-51 | -1.69E-46 |
| MS.gene056553 | MS.gene98091 | 0.809032 | 2.31E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene98091 | 0.812248 | 4.66E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene98091 | 0.802025 | 6.82E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene98091 | 0.828568 | 8.36E-55 | -1.69E-46 |
| MS.gene057693 | MS.gene98091 | 0.818263 | 2.15E-52 | -1.69E-46 |
| MS.gene057855 | MS.gene98091 | 0.802699 | 4.96E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene98091 | 0.806411 | 8.34E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene98091 | 0.833015 | 6.80E-56 | -1.69E-46 |
| MS.gene058010 | MS.gene98091 | 0.831088 | 2.03E-55 | -1.69E-46 |
| MS.gene05847 | MS.gene98091 | 0.808198 | 3.48E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene98091 | 0.846975 | 1.55E-59 | -1.69E-46 |
| MS.gene058979 | MS.gene98091 | 0.824561 | 7.55E-54 | -1.69E-46 |
| MS.gene059725 | MS.gene98091 | 0.839645 | 1.40E-57 | -1.69E-46 |
| MS.gene059842 | MS.gene98091 | 0.835251 | 1.87E-56 | -1.69E-46 |
| MS.gene059937 | MS.gene98091 | 0.812277 | 4.60E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene98091 | 0.811522 | 6.71E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene98091 | 0.825024 | 5.87E-54 | -1.69E-46 |
| MS.gene060322 | MS.gene98091 | 0.855547 | 5.86E-62 | -1.69E-46 |
| MS.gene060373 | MS.gene98091 | 0.802892 | 4.52E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene98091 | 0.824884 | 6.34E-54 | -1.69E-46 |
| MS.gene060685 | MS.gene98091 | 0.800282 | 1.55E-48 | -1.69E-46 |
| MS.gene060802 | MS.gene98091 | 0.819599 | 1.07E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene98091 | 0.841715 | 4.02E-58 | -1.69E-46 |
| MS.gene060998 | MS.gene98091 | 0.801568 | 8.47E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75628 | MS.gene98091 | PPI |
| MS.gene98091 | MS.gene72191 | PPI |
| MS.gene045301 | MS.gene98091 | PPI |
| MS.gene030447 | MS.gene98091 | PPI |
| MS.gene98091 | MS.gene77577 | PPI |
| MS.gene98091 | MS.gene000938 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98091.t1 | MTR_7g028940 | 97.101 | 69 | 2 | 0 | 1 | 69 | 1 | 69 | 4.86e-43 | 133 |
| MS.gene98091.t1 | MTR_8g096840 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 4.91e-26 | 90.9 |
| MS.gene98091.t1 | MTR_3g085020 | 57.971 | 69 | 29 | 0 | 1 | 69 | 1 | 69 | 2.82e-24 | 86.3 |
| MS.gene98091.t1 | MTR_8g097020 | 49.275 | 69 | 35 | 0 | 1 | 69 | 1 | 69 | 6.64e-22 | 80.5 |
| MS.gene98091.t1 | MTR_8g027820 | 49.275 | 69 | 35 | 0 | 1 | 69 | 1 | 69 | 2.89e-21 | 79.7 |
| MS.gene98091.t1 | MTR_7g028975 | 47.826 | 69 | 36 | 0 | 1 | 69 | 1 | 69 | 6.64e-21 | 77.8 |
| MS.gene98091.t1 | MTR_7g028870 | 42.424 | 66 | 38 | 0 | 2 | 67 | 3 | 68 | 1.03e-18 | 72.4 |
| MS.gene98091.t1 | MTR_3g085030 | 44.928 | 69 | 38 | 0 | 1 | 69 | 1 | 69 | 1.08e-18 | 72.4 |
| MS.gene98091.t1 | MTR_8g097030 | 57.353 | 68 | 29 | 0 | 2 | 69 | 3 | 70 | 2.59e-17 | 68.9 |
| MS.gene98091.t1 | MTR_7g028815 | 54.717 | 53 | 24 | 0 | 1 | 53 | 1 | 53 | 4.36e-17 | 69.3 |
| MS.gene98091.t1 | MTR_7g028945 | 40.299 | 67 | 40 | 0 | 1 | 67 | 1 | 67 | 1.44e-12 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98091.t1 | AT3G27030 | 68.116 | 69 | 22 | 0 | 1 | 69 | 74 | 142 | 1.80e-31 | 106 |
| MS.gene98091.t1 | AT3G27030 | 50.000 | 66 | 33 | 0 | 1 | 66 | 1 | 66 | 4.01e-19 | 75.5 |
| MS.gene98091.t1 | AT3G48660 | 55.072 | 69 | 31 | 0 | 1 | 69 | 1 | 69 | 4.11e-25 | 89.0 |
| MS.gene98091.t1 | AT5G63500 | 53.623 | 69 | 32 | 0 | 1 | 69 | 1 | 69 | 7.58e-24 | 85.1 |
| MS.gene98091.t1 | AT5G08391 | 50.725 | 69 | 34 | 0 | 1 | 69 | 1 | 69 | 4.74e-22 | 80.9 |
| MS.gene98091.t1 | AT5G14110 | 55.224 | 67 | 30 | 0 | 3 | 69 | 4 | 70 | 2.09e-21 | 79.3 |
| MS.gene98091.t1 | AT3G01950 | 49.254 | 67 | 34 | 0 | 3 | 69 | 4 | 70 | 3.82e-21 | 78.6 |
| MS.gene98091.t1 | AT3G27027 | 42.424 | 66 | 38 | 0 | 2 | 67 | 3 | 68 | 7.68e-19 | 72.8 |
| MS.gene98091.t1 | AT5G40980 | 45.283 | 53 | 29 | 0 | 2 | 54 | 3 | 55 | 4.82e-15 | 63.2 |
| MS.gene98091.t1 | AT3G01940 | 43.939 | 66 | 37 | 0 | 2 | 67 | 4 | 69 | 9.65e-13 | 57.4 |
| MS.gene98091.t1 | AT5G50560 | 47.619 | 63 | 33 | 0 | 1 | 63 | 1 | 63 | 9.13e-12 | 54.7 |
| MS.gene98091.t1 | AT5G50660 | 47.619 | 63 | 33 | 0 | 1 | 63 | 1 | 63 | 9.13e-12 | 54.7 |
| MS.gene98091.t1 | AT5G40960 | 40.299 | 67 | 40 | 0 | 1 | 67 | 1 | 67 | 1.05e-11 | 54.7 |
Find 11 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTGGGCACCAGTGGTTAT+TGG | 0.332087 | 7.2:+70928971 | MS.gene98091:CDS |
| TCTCTATGCTGTTATCATCT+TGG | 0.333421 | 7.2:+70929123 | MS.gene98091:CDS |
| CTTCATGTTCATATCTATAC+TGG | 0.339150 | 7.2:+70929148 | MS.gene98091:CDS |
| AATGGAAAACAGCTTGAGTT+TGG | 0.402355 | 7.2:+70929046 | MS.gene98091:CDS |
| CTGTTTGTGTTTCTTCAACC+AGG | 0.411752 | 7.2:+70929001 | MS.gene98091:CDS |
| TTTGGTGGTATGAAGACAAA+TGG | 0.496601 | 7.2:+70929064 | MS.gene98091:CDS |
| GGAAAACAGCTTGAGTTTGG+TGG | 0.507655 | 7.2:+70929049 | MS.gene98091:CDS |
| TGTTTGTGTTTCTTCAACCA+GGG | 0.532155 | 7.2:+70929002 | MS.gene98091:CDS |
| AATGTCTGATTGGGCACCAG+TGG | 0.594590 | 7.2:+70928964 | None:intergenic |
| AGCACAACTCCAATAACCAC+TGG | 0.602053 | 7.2:-70928980 | None:intergenic |
| GGAAAAGCAAACAACAACCC+TGG | 0.665269 | 7.2:-70929019 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCATACACTTATTTTCTT+TGG | + | chr7.2:70929100-70929119 | MS.gene98091:CDS | 20.0% |
| CTTCATGTTCATATCTATAC+TGG | + | chr7.2:70929148-70929167 | MS.gene98091:CDS | 30.0% | |
| AATGGAAAACAGCTTGAGTT+TGG | + | chr7.2:70929046-70929065 | MS.gene98091:CDS | 35.0% | |
| TCTCTATGCTGTTATCATCT+TGG | + | chr7.2:70929123-70929142 | MS.gene98091:CDS | 35.0% | |
| TGTTTGTGTTTCTTCAACCA+GGG | + | chr7.2:70929002-70929021 | MS.gene98091:CDS | 35.0% | |
| ! | TTTGGTGGTATGAAGACAAA+TGG | + | chr7.2:70929064-70929083 | MS.gene98091:CDS | 35.0% |
| !! | TTGTTTGCTTTTCCAGGAAA+TGG | + | chr7.2:70929028-70929047 | MS.gene98091:CDS | 35.0% |
| CTGTTTGTGTTTCTTCAACC+AGG | + | chr7.2:70929001-70929020 | MS.gene98091:CDS | 40.0% | |
| !! | TCAAGCTGTTTTCCATTTCC+TGG | - | chr7.2:70929043-70929062 | None:intergenic | 40.0% |
| AGCACAACTCCAATAACCAC+TGG | - | chr7.2:70928983-70929002 | None:intergenic | 45.0% | |
| GGAAAACAGCTTGAGTTTGG+TGG | + | chr7.2:70929049-70929068 | MS.gene98091:CDS | 45.0% | |
| GGAAAAGCAAACAACAACCC+TGG | - | chr7.2:70929022-70929041 | None:intergenic | 45.0% | |
| !!! | GGGTTGTTGTTTGCTTTTCC+AGG | + | chr7.2:70929022-70929041 | MS.gene98091:CDS | 45.0% |
| GATTGGGCACCAGTGGTTAT+TGG | + | chr7.2:70928971-70928990 | MS.gene98091:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 70928965 | 70929174 | 70928965 | ID=MS.gene98091 |
| chr7.2 | mRNA | 70928965 | 70929174 | 70928965 | ID=MS.gene98091.t1;Parent=MS.gene98091 |
| chr7.2 | exon | 70928965 | 70929174 | 70928965 | ID=MS.gene98091.t1.exon1;Parent=MS.gene98091.t1 |
| chr7.2 | CDS | 70928965 | 70929174 | 70928965 | ID=cds.MS.gene98091.t1;Parent=MS.gene98091.t1 |
| Gene Sequence |
| Protein sequence |
