AlfalfaGEDB | MS.gene002025

M. sativa cultivar XinJiangDaYe / MS.gene002025

KEGG: -
GO: -
PFAM: PF05078.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002025.t1	PNX88095.1	81	189	27	4	45	232	41	221	3.30E-67	265

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002025.t1	Q9STW3	55.3	179	70	3	47	225	39	207	3.8e-48	193.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002025.t1	A0A2K3MBD2	81.0	189	27	4	45	232	41	221	2.4e-67	265.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene002025	MS.gene32027	PPI
MS.gene002025	MS.gene002504	PPI
MS.gene01477	MS.gene002025	PPI
MS.gene004174	MS.gene002025	PPI
MS.gene02809	MS.gene002025	PPI
MS.gene02810	MS.gene002025	PPI
MS.gene39522	MS.gene002025	PPI
MS.gene43093	MS.gene002025	PPI
MS.gene72970	MS.gene002025	PPI
MS.gene047359	MS.gene002025	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002025.t1	MTR_4g088335	47.917	192	86	4	35	225	26	204	2.83e-60	189
MS.gene002025.t1	MTR_2g014550	48.187	193	85	3	36	225	28	208	3.58e-60	188
MS.gene002025.t1	MTR_2g014520	47.120	191	89	2	35	225	27	205	1.01e-58	184
MS.gene002025.t1	MTR_4g078870	43.784	185	89	4	43	225	32	203	1.48e-48	159
MS.gene002025.t1	MTR_2g020850	39.691	194	102	3	36	229	13	191	1.12e-43	146
MS.gene002025.t1	MTR_5g044580	39.683	189	99	4	36	221	38	214	1.96e-41	140
MS.gene002025.t1	MTR_7g010890	36.792	212	114	5	13	221	20	214	2.72e-40	138
MS.gene002025.t1	MTR_4g118480	36.098	205	117	4	21	225	5	195	5.91e-38	132
MS.gene002025.t1	MTR_4g118470	39.344	183	97	4	43	225	29	197	8.80e-38	131

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002025.t1	AT4G24310	58.854	192	69	3	34	225	26	207	1.28e-69	213
MS.gene002025.t1	AT3G02430	56.771	192	73	3	34	225	32	213	2.81e-69	212
MS.gene002025.t1	AT4G18425	49.153	177	76	4	50	225	48	211	1.21e-53	172
MS.gene002025.t1	AT5G46090	45.078	193	92	4	34	225	33	212	2.87e-52	168
MS.gene002025.t1	AT4G28485	44.670	197	94	5	32	225	14	198	5.41e-45	149
MS.gene002025.t1	AT3G21550	42.614	176	84	3	48	223	14	172	4.86e-39	134
MS.gene002025.t1	AT1G09157	38.889	180	108	2	43	221	61	239	6.18e-37	130
MS.gene002025.t1	AT3G21520	38.418	177	94	3	46	222	26	187	4.04e-36	127
MS.gene002025.t1	AT5G39650	38.889	180	108	2	43	221	62	240	4.17e-36	128
MS.gene002025.t1	AT5G27370	37.705	183	103	5	46	221	8	186	1.71e-29	109
MS.gene002025.t1	AT4G28485	44.545	110	50	2	116	225	65	163	6.98e-20	83.6

Find 59 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTTCAGATTGCTTATTAT+TGG	0.102422	2.3:-76028878	None:intergenic
GTACTCCTAGTAATTATATT+TGG	0.194804	2.3:-76029544	None:intergenic
CTAGTAATTATATTTGGAAT+TGG	0.267098	2.3:-76029538	None:intergenic
TGTTCTTTGCTGTTGCATTA+AGG	0.305960	2.3:+76029358	MS.gene002025:CDS
GAATTGGGTGCAGGTGTAAC+AGG	0.307857	2.3:-76029522	None:intergenic
GATATTGTGCCCCTCGGAAT+TGG	0.326356	2.3:+76029441	MS.gene002025:CDS
TGAAGACTGGCGTGAGAATC+TGG	0.355118	2.3:-76029050	None:intergenic
TAGTCACACGGCGTGGTTGC+GGG	0.359159	2.3:-76029407	None:intergenic
GTTCTTTGCTGTTGCATTAA+GGG	0.365115	2.3:+76029359	MS.gene002025:CDS
ATGCTTCCTTGCTTGTTTCA+CGG	0.371761	2.3:+76029143	MS.gene002025:CDS
TAGTAATTATATTTGGAATT+GGG	0.375012	2.3:-76029537	None:intergenic
CCATGGCATCGCAACCTTCA+AGG	0.392282	2.3:+76029200	MS.gene002025:CDS
GTAGTATCTGGAGCATTAAC+GGG	0.406461	2.3:+76028979	MS.gene002025:CDS
AAATGCATGTACAAAGTCAA+TGG	0.433016	2.3:-76029322	None:intergenic
AGTAGTATCTGGAGCATTAA+CGG	0.435471	2.3:+76028978	MS.gene002025:CDS
CGACGGTCAGTAGTAGTATC+TGG	0.437746	2.3:+76028967	MS.gene002025:CDS
TTAGTCACACGGCGTGGTTG+CGG	0.444013	2.3:-76029408	None:intergenic
TCGCAACCTTCAAGGGTCTC+TGG	0.469635	2.3:+76029208	MS.gene002025:CDS
GCAGCAGCACATGGAATTAG+TGG	0.477735	2.3:+76028931	MS.gene002025:CDS
CAATTCCAAATATAATTACT+AGG	0.486361	2.3:+76029539	MS.gene002025:CDS
GCAACCACGCCGTGTGACTA+AGG	0.489093	2.3:+76029410	MS.gene002025:CDS
TTATATTTGGAATTGGGTGC+AGG	0.489229	2.3:-76029531	None:intergenic
AATAATAAGCAATCTGAAGC+AGG	0.489383	2.3:+76028880	MS.gene002025:CDS
CATGGCATCGCAACCTTCAA+GGG	0.499059	2.3:+76029201	MS.gene002025:CDS
GCTCCGTTGTTTGTGAAGAC+TGG	0.503057	2.3:-76029063	None:intergenic
AAATAGCCAGAGACCCTTGA+AGG	0.509853	2.3:-76029214	None:intergenic
TTATACTTGCTCAACTCAGC+TGG	0.520275	2.3:-76029291	None:intergenic
GGGAAGTAAGTTTGCCAAGT+TGG	0.532415	2.3:-76029007	None:intergenic
CCAACCACAAGACATGGGAT+TGG	0.533310	2.3:+76029495	MS.gene002025:CDS
AATGCATGTACAAAGTCAAT+GGG	0.533496	2.3:-76029321	None:intergenic
GCGTGAGAATCTGGAATGCT+AGG	0.537937	2.3:-76029041	None:intergenic
ATTAACGGGGACAGCCAACT+TGG	0.539398	2.3:+76028993	MS.gene002025:CDS
TCCATGACATTGACATTGAC+GGG	0.550083	2.3:-76029246	None:intergenic
AGTTGAGCAAGTATAAGATC+AGG	0.557038	2.3:+76029298	MS.gene002025:CDS
CCAATCCCATGTCTTGTGGT+TGG	0.557682	2.3:-76029495	None:intergenic
GTAGCCAATCCCATGTCTTG+TGG	0.558071	2.3:-76029499	None:intergenic
GTCCATGACATTGACATTGA+CGG	0.563837	2.3:-76029247	None:intergenic
GTTCTTGATATTGTGCCCCT+CGG	0.569071	2.3:+76029435	MS.gene002025:CDS
CCTTGAAGGTTGCGATGCCA+TGG	0.569779	2.3:-76029200	None:intergenic
ACCCGTCAATGTCAATGTCA+TGG	0.570259	2.3:+76029245	MS.gene002025:CDS
ACAGTGAATAATGCATCAGA+TGG	0.586121	2.3:+76029168	MS.gene002025:CDS
TTGGCAAACTTACTTCCCAC+AGG	0.590824	2.3:+76029012	MS.gene002025:CDS
GAATGCTAGGAGTGTTCCTG+TGG	0.592747	2.3:-76029028	None:intergenic
GCAAAGGAATCCAATTCCGA+GGG	0.606112	2.3:-76029451	None:intergenic
TGCAAAGGAATCCAATTCCG+AGG	0.607762	2.3:-76029452	None:intergenic
ATGAGATTGCAAGCAAGATG+AGG	0.612498	2.3:-76029122	None:intergenic
AATAAGCAATCTGAAGCAGG+TGG	0.615428	2.3:+76028883	MS.gene002025:CDS
AAGTGTGAGAATGCGAGTAA+CGG	0.616907	2.3:-76029094	None:intergenic
CAAGAACCTCCTTAGTCACA+CGG	0.627426	2.3:-76029419	None:intergenic
CTGTGTCCGTGAAACAAGCA+AGG	0.639798	2.3:-76029149	None:intergenic
AATTGGGTGCAGGTGTAACA+GGG	0.642036	2.3:-76029521	None:intergenic
ACCTCCTTAGTCACACGGCG+TGG	0.648095	2.3:-76029414	None:intergenic
ACGCCAGTCTTCACAAACAA+CGG	0.649231	2.3:+76029060	MS.gene002025:CDS
GTGGTAGACGACGACGACGA+CGG	0.670485	2.3:+76028950	MS.gene002025:CDS
TAGTATCTGGAGCATTAACG+GGG	0.687598	2.3:+76028980	MS.gene002025:CDS
TCAGATGGAAGAATCTACCA+TGG	0.695565	2.3:+76029183	MS.gene002025:CDS
ACCACGCCGTGTGACTAAGG+AGG	0.698128	2.3:+76029413	MS.gene002025:CDS
AATGCTAGGAGTGTTCCTGT+GGG	0.719785	2.3:-76029027	None:intergenic
CAAAGGAATCCAATTCCGAG+GGG	0.755197	2.3:-76029450	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAGTAATTATATTTGGAATT+GGG	-	chr2.3:76029540-76029559	None:intergenic	15.0%
!!!	CTAGTAATTATATTTGGAAT+TGG	-	chr2.3:76029541-76029560	None:intergenic	20.0%
	AAATGCATGTACAAAGTCAA+TGG	-	chr2.3:76029325-76029344	None:intergenic	30.0%
	AATGCATGTACAAAGTCAAT+GGG	-	chr2.3:76029324-76029343	None:intergenic	30.0%
	TGCTTCAGATTGCTTATTAT+TGG	-	chr2.3:76028881-76028900	None:intergenic	30.0%
!	AATAATAAGCAATCTGAAGC+AGG	+	chr2.3:76028880-76028899	MS.gene002025:CDS	30.0%
	ACAGTGAATAATGCATCAGA+TGG	+	chr2.3:76029168-76029187	MS.gene002025:CDS	35.0%
	AGTTGAGCAAGTATAAGATC+AGG	+	chr2.3:76029298-76029317	MS.gene002025:CDS	35.0%
!	AGTAGTATCTGGAGCATTAA+CGG	+	chr2.3:76028978-76028997	MS.gene002025:CDS	35.0%
!	TTATATTTGGAATTGGGTGC+AGG	-	chr2.3:76029534-76029553	None:intergenic	35.0%
!	TTTTTCCAACCACAAGACAT+GGG	+	chr2.3:76029490-76029509	MS.gene002025:CDS	35.0%
!!	GTTCTTTGCTGTTGCATTAA+GGG	+	chr2.3:76029359-76029378	MS.gene002025:CDS	35.0%
!!	TGTTCTTTGCTGTTGCATTA+AGG	+	chr2.3:76029358-76029377	MS.gene002025:CDS	35.0%
	AAGTGTGAGAATGCGAGTAA+CGG	-	chr2.3:76029097-76029116	None:intergenic	40.0%
	ATGAGATTGCAAGCAAGATG+AGG	-	chr2.3:76029125-76029144	None:intergenic	40.0%
	ATGCTTCCTTGCTTGTTTCA+CGG	+	chr2.3:76029143-76029162	MS.gene002025:CDS	40.0%
	CGACAAAAAGCAAAGTGCAA+AGG	-	chr2.3:76029470-76029489	None:intergenic	40.0%
	GTCCATGACATTGACATTGA+CGG	-	chr2.3:76029250-76029269	None:intergenic	40.0%
	TCAGATGGAAGAATCTACCA+TGG	+	chr2.3:76029183-76029202	MS.gene002025:CDS	40.0%
	TCCATGACATTGACATTGAC+GGG	-	chr2.3:76029249-76029268	None:intergenic	40.0%
	TTATACTTGCTCAACTCAGC+TGG	-	chr2.3:76029294-76029313	None:intergenic	40.0%
!	AATAAGCAATCTGAAGCAGG+TGG	+	chr2.3:76028883-76028902	MS.gene002025:CDS	40.0%
!	GTAGTATCTGGAGCATTAAC+GGG	+	chr2.3:76028979-76028998	MS.gene002025:CDS	40.0%
!	GTTTTTCCAACCACAAGACA+TGG	+	chr2.3:76029489-76029508	MS.gene002025:CDS	40.0%
!	TAGTATCTGGAGCATTAACG+GGG	+	chr2.3:76028980-76028999	MS.gene002025:CDS	40.0%
	AAATAGCCAGAGACCCTTGA+AGG	-	chr2.3:76029217-76029236	None:intergenic	45.0%
	AATTGGGTGCAGGTGTAACA+GGG	-	chr2.3:76029524-76029543	None:intergenic	45.0%
	ACCCGTCAATGTCAATGTCA+TGG	+	chr2.3:76029245-76029264	MS.gene002025:CDS	45.0%
	ACGCCAGTCTTCACAAACAA+CGG	+	chr2.3:76029060-76029079	MS.gene002025:CDS	45.0%
	CAAAGGAATCCAATTCCGAG+GGG	-	chr2.3:76029453-76029472	None:intergenic	45.0%
	CAAGAACCTCCTTAGTCACA+CGG	-	chr2.3:76029422-76029441	None:intergenic	45.0%
	GCAAAGGAATCCAATTCCGA+GGG	-	chr2.3:76029454-76029473	None:intergenic	45.0%
	GGGAAGTAAGTTTGCCAAGT+TGG	-	chr2.3:76029010-76029029	None:intergenic	45.0%
	GTTCTTGATATTGTGCCCCT+CGG	+	chr2.3:76029435-76029454	MS.gene002025:CDS	45.0%
	TGCAAAGGAATCCAATTCCG+AGG	-	chr2.3:76029455-76029474	None:intergenic	45.0%
	TTGGCAAACTTACTTCCCAC+AGG	+	chr2.3:76029012-76029031	MS.gene002025:CDS	45.0%
!!	AATGCTAGGAGTGTTCCTGT+GGG	-	chr2.3:76029030-76029049	None:intergenic	45.0%
	ATTAACGGGGACAGCCAACT+TGG	+	chr2.3:76028993-76029012	MS.gene002025:CDS	50.0%
	CATGGCATCGCAACCTTCAA+GGG	+	chr2.3:76029201-76029220	MS.gene002025:CDS	50.0%
	CCAACCACAAGACATGGGAT+TGG	+	chr2.3:76029495-76029514	MS.gene002025:CDS	50.0%
	CCAATCCCATGTCTTGTGGT+TGG	-	chr2.3:76029498-76029517	None:intergenic	50.0%
	CGACGGTCAGTAGTAGTATC+TGG	+	chr2.3:76028967-76028986	MS.gene002025:CDS	50.0%
	CTGTGTCCGTGAAACAAGCA+AGG	-	chr2.3:76029152-76029171	None:intergenic	50.0%
	GAATTGGGTGCAGGTGTAAC+AGG	-	chr2.3:76029525-76029544	None:intergenic	50.0%
	GATATTGTGCCCCTCGGAAT+TGG	+	chr2.3:76029441-76029460	MS.gene002025:CDS	50.0%
	GCAGCAGCACATGGAATTAG+TGG	+	chr2.3:76028931-76028950	MS.gene002025:CDS	50.0%
	GCTCCGTTGTTTGTGAAGAC+TGG	-	chr2.3:76029066-76029085	None:intergenic	50.0%
	GTAGCCAATCCCATGTCTTG+TGG	-	chr2.3:76029502-76029521	None:intergenic	50.0%
	TGAAGACTGGCGTGAGAATC+TGG	-	chr2.3:76029053-76029072	None:intergenic	50.0%
!	GCGTGAGAATCTGGAATGCT+AGG	-	chr2.3:76029044-76029063	None:intergenic	50.0%
!!	GAATGCTAGGAGTGTTCCTG+TGG	-	chr2.3:76029031-76029050	None:intergenic	50.0%
	CCATGGCATCGCAACCTTCA+AGG	+	chr2.3:76029200-76029219	MS.gene002025:CDS	55.0%
	CCTTGAAGGTTGCGATGCCA+TGG	-	chr2.3:76029203-76029222	None:intergenic	55.0%
	TCGCAACCTTCAAGGGTCTC+TGG	+	chr2.3:76029208-76029227	MS.gene002025:CDS	55.0%
	TTAGTCACACGGCGTGGTTG+CGG	-	chr2.3:76029411-76029430	None:intergenic	55.0%
	ACCACGCCGTGTGACTAAGG+AGG	+	chr2.3:76029413-76029432	MS.gene002025:CDS	60.0%
	ACCTCCTTAGTCACACGGCG+TGG	-	chr2.3:76029417-76029436	None:intergenic	60.0%
	GCAACCACGCCGTGTGACTA+AGG	+	chr2.3:76029410-76029429	MS.gene002025:CDS	60.0%
	GTGGTAGACGACGACGACGA+CGG	+	chr2.3:76028950-76028969	MS.gene002025:CDS	60.0%
	TAGTCACACGGCGTGGTTGC+GGG	-	chr2.3:76029410-76029429	None:intergenic	60.0%
	TCAGCAGCAGCAGCAGCACA+TGG	+	chr2.3:76028922-76028941	MS.gene002025:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	76028856	76029560	76028856	ID=MS.gene002025
chr2.3	mRNA	76028856	76029560	76028856	ID=MS.gene002025.t1;Parent=MS.gene002025
chr2.3	exon	76028856	76029560	76028856	ID=MS.gene002025.t1.exon1;Parent=MS.gene002025.t1
chr2.3	CDS	76028856	76029560	76028856	ID=cds.MS.gene002025.t1;Parent=MS.gene002025.t1

Gene Sequence

>MS.gene002025

ATGTCGGTTACACAACGTCGATCCAATAATAAGCAATCTGAAGCAGGTGGATCATCATCATCAGCATCAGCAGCAGCAGCAGCACATGGAATTAGTGGTAGACGACGACGACGACGGTCAGTAGTAGTATCTGGAGCATTAACGGGGACAGCCAACTTGGCAAACTTACTTCCCACAGGAACACTCCTAGCATTCCAGATTCTCACGCCAGTCTTCACAAACAACGGAGCTTGTGACTCCGTTACTCGCATTCTCACACTTGTACTCCTCATCTTGCTTGCAATCTCATGCTTCCTTGCTTGTTTCACGGACACAGTGAATAATGCATCAGATGGAAGAATCTACCATGGCATCGCAACCTTCAAGGGTCTCTGGCTATTTGATTACTCACCCGTCAATGTCAATGTCATGGACAGTAGTAGTACAATAATATTACCAGCTGAGTTGAGCAAGTATAAGATCAGGCCCATTGACTTTGTACATGCATTTTTGTCTGTCTTTGTGTTCTTTGCTGTTGCATTAAGGGATAACAATGTGTTGACATGTTATTACCCGCAACCACGCCGTGTGACTAAGGAGGTTCTTGATATTGTGCCCCTCGGAATTGGATTCCTTTGCACTTTGCTTTTTGTCGTTTTTCCAACCACAAGACATGGGATTGGCTACCCTGTTACACCTGCACCCAATTCCAAATATAATTACTAG

Protein sequence

>MS.gene002025.t1

MSVTQRRSNNKQSEAGGSSSSASAAAAAHGISGRRRRRRSVVVSGALTGTANLANLLPTGTLLAFQILTPVFTNNGACDSVTRILTLVLLILLAISCFLACFTDTVNNASDGRIYHGIATFKGLWLFDYSPVNVNVMDSSSTIILPAELSKYKIRPIDFVHAFLSVFVFFAVALRDNNVLTCYYPQPRRVTKEVLDIVPLGIGFLCTLLFVVFPTTRHGIGYPVTPAPNSKYNY