Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047359.t1 | XP_003593648.1 | 97.6 | 211 | 4 | 1 | 1 | 211 | 1 | 210 | 2.00E-108 | 401.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047359.t1 | Q9FNL3 | 61.7 | 214 | 75 | 3 | 1 | 209 | 1 | 212 | 5.9e-72 | 271.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047359.t1 | A2Q514 | 97.6 | 211 | 4 | 1 | 1 | 211 | 1 | 210 | 1.5e-108 | 401.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene072079 | MS.gene047359 | PPI |
| MS.gene047359 | MS.gene047221 | PPI |
| MS.gene047359 | MS.gene002025 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047359.t1 | MTR_2g014550 | 97.630 | 211 | 4 | 1 | 1 | 211 | 1 | 210 | 1.00e-151 | 419 |
| MS.gene047359.t1 | MTR_2g014520 | 87.923 | 207 | 25 | 0 | 5 | 211 | 1 | 207 | 8.15e-135 | 376 |
| MS.gene047359.t1 | MTR_4g088335 | 73.604 | 197 | 52 | 0 | 15 | 211 | 10 | 206 | 1.24e-109 | 313 |
| MS.gene047359.t1 | MTR_4g078870 | 54.922 | 193 | 81 | 3 | 20 | 209 | 14 | 203 | 1.44e-75 | 226 |
| MS.gene047359.t1 | MTR_5g044580 | 43.094 | 181 | 101 | 2 | 26 | 205 | 35 | 214 | 5.19e-49 | 159 |
| MS.gene047359.t1 | MTR_2g020850 | 38.947 | 190 | 109 | 2 | 25 | 210 | 2 | 188 | 2.56e-46 | 152 |
| MS.gene047359.t1 | MTR_7g010890 | 41.436 | 181 | 104 | 2 | 26 | 205 | 35 | 214 | 4.74e-46 | 152 |
| MS.gene047359.t1 | MTR_4g118470 | 41.361 | 191 | 98 | 3 | 24 | 210 | 18 | 198 | 5.82e-43 | 144 |
| MS.gene047359.t1 | MTR_4g118480 | 42.045 | 176 | 92 | 2 | 39 | 210 | 27 | 196 | 9.21e-41 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047359.t1 | AT5G46090 | 61.682 | 214 | 75 | 3 | 1 | 209 | 1 | 212 | 2.01e-97 | 282 |
| MS.gene047359.t1 | AT4G18425 | 59.434 | 212 | 82 | 3 | 3 | 210 | 1 | 212 | 4.42e-92 | 269 |
| MS.gene047359.t1 | AT4G28485 | 50.254 | 197 | 96 | 2 | 15 | 209 | 2 | 198 | 7.52e-67 | 204 |
| MS.gene047359.t1 | AT3G02430 | 47.396 | 192 | 95 | 2 | 24 | 211 | 26 | 215 | 1.88e-64 | 199 |
| MS.gene047359.t1 | AT4G24310 | 50.000 | 192 | 89 | 3 | 23 | 209 | 18 | 207 | 5.76e-61 | 190 |
| MS.gene047359.t1 | AT1G09157 | 38.587 | 184 | 101 | 1 | 34 | 205 | 56 | 239 | 1.70e-42 | 143 |
| MS.gene047359.t1 | AT3G21550 | 43.605 | 172 | 91 | 2 | 37 | 207 | 6 | 172 | 5.16e-42 | 140 |
| MS.gene047359.t1 | AT5G39650 | 38.172 | 186 | 99 | 2 | 34 | 205 | 57 | 240 | 6.10e-42 | 142 |
| MS.gene047359.t1 | AT3G21520 | 40.000 | 160 | 89 | 2 | 49 | 206 | 33 | 187 | 5.67e-36 | 125 |
| MS.gene047359.t1 | AT4G28485 | 56.566 | 99 | 43 | 0 | 111 | 209 | 65 | 163 | 1.55e-33 | 118 |
| MS.gene047359.t1 | AT5G27370 | 35.227 | 176 | 99 | 3 | 45 | 205 | 11 | 186 | 2.58e-30 | 110 |
Find 43 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAATTAACTTGTTGAAAG+AGG | 0.329827 | 2.3:-70330112 | None:intergenic |
| ATTTCAGAGCACGGCGCATT+TGG | 0.350269 | 2.3:+70329619 | MS.gene047359:CDS |
| AGCAAGGGGAATATTTGTTA+TGG | 0.356880 | 2.3:+70329806 | MS.gene047359:CDS |
| AAAGGTTTGTGGGTCATTGA+TGG | 0.367892 | 2.3:+70329842 | MS.gene047359:CDS |
| GCAAGGGGAATATTTGTTAT+GGG | 0.374957 | 2.3:+70329807 | MS.gene047359:CDS |
| ACGGCGTTGCCTGTGGCAAT+TGG | 0.380489 | 2.3:+70330031 | MS.gene047359:CDS |
| CCTACTGAGAGACATGGAAT+TGG | 0.411080 | 2.3:+70330085 | MS.gene047359:CDS |
| ACCAGGCTCCTCTCTGCCTC+TGG | 0.411547 | 2.3:-70329575 | None:intergenic |
| TATGGGTTTGCCACCTTCAA+AGG | 0.413754 | 2.3:+70329824 | MS.gene047359:CDS |
| TGCCACCTTCAAAGGTTTGT+GGG | 0.416898 | 2.3:+70329832 | MS.gene047359:CDS |
| AGCAACTTGAGGTGGAAGTT+TGG | 0.423290 | 2.3:-70329870 | None:intergenic |
| AACTTATAGCTTTCTGTACC+AGG | 0.430636 | 2.3:-70329592 | None:intergenic |
| GCATGCGGTGATGTCAATTC+TGG | 0.434994 | 2.3:+70329925 | MS.gene047359:CDS |
| GAACAGTGCCTGTAGGTAGA+AGG | 0.449758 | 2.3:-70329646 | None:intergenic |
| AATGACCCACAAACCTTTGA+AGG | 0.450179 | 2.3:-70329837 | None:intergenic |
| TTGCCACCTTCAAAGGTTTG+TGG | 0.452480 | 2.3:+70329831 | MS.gene047359:CDS |
| TGCTATCAGTAAAGTTTAGC+AGG | 0.458079 | 2.3:-70329775 | None:intergenic |
| TTAATTAACTTGTTGAAAGA+GGG | 0.489952 | 2.3:-70330111 | None:intergenic |
| TAGGAATGACGATGAACAAA+AGG | 0.495588 | 2.3:+70329526 | MS.gene047359:CDS |
| TTGGCAAACCTTCTACCTAC+AGG | 0.528525 | 2.3:+70329638 | MS.gene047359:CDS |
| TCTGTACTTGTGACTCTATG+TGG | 0.546587 | 2.3:+70329740 | MS.gene047359:CDS |
| TCCAGAGGCAGAGAGGAGCC+TGG | 0.549208 | 2.3:+70329574 | MS.gene047359:CDS |
| CCAATTCCATGTCTCTCAGT+AGG | 0.555703 | 2.3:-70330085 | None:intergenic |
| TAGAAATCGATGATGACTCT+AGG | 0.560367 | 2.3:+70329507 | MS.gene047359:CDS |
| AGTCACAAGTACAGAAGTCA+TGG | 0.564650 | 2.3:-70329732 | None:intergenic |
| TCCACTTCTGCGGAATGCGG+AGG | 0.572373 | 2.3:+70329550 | MS.gene047359:CDS |
| ACCTCCGCATTCCGCAGAAG+TGG | 0.578568 | 2.3:-70329551 | None:intergenic |
| TGATAGCATTAGAGACAGCA+AGG | 0.586072 | 2.3:+70329790 | MS.gene047359:CDS |
| GTTTATAGATTTCATGCATG+CGG | 0.586781 | 2.3:+70329910 | MS.gene047359:CDS |
| AACAAAAGGTTCCACTTCTG+CGG | 0.591604 | 2.3:+70329540 | MS.gene047359:CDS |
| AAGTTCGACATTTCAGAGCA+CGG | 0.595642 | 2.3:+70329610 | MS.gene047359:CDS |
| AATCCTCACGGCGTTGCCTG+TGG | 0.613810 | 2.3:+70330024 | MS.gene047359:CDS |
| TTGCCACAGGCAACGCCGTG+AGG | 0.614771 | 2.3:-70330027 | None:intergenic |
| GCGGAATGCGGAGGTTCCAG+AGG | 0.622187 | 2.3:+70329559 | MS.gene047359:CDS |
| TGCATTGTTTGATCAGAATG+TGG | 0.627363 | 2.3:+70329961 | MS.gene047359:CDS |
| GGTTCCACTTCTGCGGAATG+CGG | 0.628493 | 2.3:+70329547 | MS.gene047359:CDS |
| CGGAGGTTCCAGAGGCAGAG+AGG | 0.628788 | 2.3:+70329567 | MS.gene047359:CDS |
| GCATTTCCTACTGAGAGACA+TGG | 0.635534 | 2.3:+70330079 | MS.gene047359:CDS |
| GATAGCATTAGAGACAGCAA+GGG | 0.637719 | 2.3:+70329791 | MS.gene047359:CDS |
| GACCCACAAACCTTTGAAGG+TGG | 0.639111 | 2.3:-70329834 | None:intergenic |
| AATGCAAGAACAGTGCCTGT+AGG | 0.674988 | 2.3:-70329653 | None:intergenic |
| AGAGATACAAGAAATCCTCA+CGG | 0.689697 | 2.3:+70330012 | MS.gene047359:CDS |
| ATAGCATTAGAGACAGCAAG+GGG | 0.732552 | 2.3:+70329792 | MS.gene047359:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGCCAACATTTGTAAAAAT+TGG | - | chr2.3:70329692-70329711 | None:intergenic | 25.0% |
| !! | ATTTCTTGTATCTCTTTTGA+TGG | - | chr2.3:70330007-70330026 | None:intergenic | 25.0% |
| !! | TAAAAATTGGTGACAAAAGT+TGG | - | chr2.3:70329679-70329698 | None:intergenic | 25.0% |
| !! | TCACCAATTTTTACAAATGT+TGG | + | chr2.3:70329686-70329705 | MS.gene047359:CDS | 25.0% |
| GTTTATAGATTTCATGCATG+CGG | + | chr2.3:70329910-70329929 | MS.gene047359:CDS | 30.0% | |
| AACTTATAGCTTTCTGTACC+AGG | - | chr2.3:70329595-70329614 | None:intergenic | 35.0% | |
| AGAGATACAAGAAATCCTCA+CGG | + | chr2.3:70330012-70330031 | MS.gene047359:CDS | 35.0% | |
| TAGAAATCGATGATGACTCT+AGG | + | chr2.3:70329507-70329526 | MS.gene047359:CDS | 35.0% | |
| TAGGAATGACGATGAACAAA+AGG | + | chr2.3:70329526-70329545 | MS.gene047359:CDS | 35.0% | |
| TGCATTGTTTGATCAGAATG+TGG | + | chr2.3:70329961-70329980 | MS.gene047359:CDS | 35.0% | |
| TGCTATCAGTAAAGTTTAGC+AGG | - | chr2.3:70329778-70329797 | None:intergenic | 35.0% | |
| ! | AGCAAGGGGAATATTTGTTA+TGG | + | chr2.3:70329806-70329825 | MS.gene047359:CDS | 35.0% |
| !! | GCAAGGGGAATATTTGTTAT+GGG | + | chr2.3:70329807-70329826 | MS.gene047359:CDS | 35.0% |
| !! | TGTATCTCTTTTGATGGTTC+TGG | - | chr2.3:70330001-70330020 | None:intergenic | 35.0% |
| AAAGGTTTGTGGGTCATTGA+TGG | + | chr2.3:70329842-70329861 | MS.gene047359:CDS | 40.0% | |
| AACAAAAGGTTCCACTTCTG+CGG | + | chr2.3:70329540-70329559 | MS.gene047359:CDS | 40.0% | |
| AAGTTCGACATTTCAGAGCA+CGG | + | chr2.3:70329610-70329629 | MS.gene047359:CDS | 40.0% | |
| AATGACCCACAAACCTTTGA+AGG | - | chr2.3:70329840-70329859 | None:intergenic | 40.0% | |
| AGTCACAAGTACAGAAGTCA+TGG | - | chr2.3:70329735-70329754 | None:intergenic | 40.0% | |
| ATAGCATTAGAGACAGCAAG+GGG | + | chr2.3:70329792-70329811 | MS.gene047359:CDS | 40.0% | |
| GATAGCATTAGAGACAGCAA+GGG | + | chr2.3:70329791-70329810 | MS.gene047359:CDS | 40.0% | |
| TCTGTACTTGTGACTCTATG+TGG | + | chr2.3:70329740-70329759 | MS.gene047359:CDS | 40.0% | |
| TGATAGCATTAGAGACAGCA+AGG | + | chr2.3:70329790-70329809 | MS.gene047359:CDS | 40.0% | |
| ! | CGATATTTTGCAGCAACTTG+AGG | - | chr2.3:70329884-70329903 | None:intergenic | 40.0% |
| ! | TATTTTGCAGCAACTTGAGG+TGG | - | chr2.3:70329881-70329900 | None:intergenic | 40.0% |
| AATGCAAGAACAGTGCCTGT+AGG | - | chr2.3:70329656-70329675 | None:intergenic | 45.0% | |
| AGCAACTTGAGGTGGAAGTT+TGG | - | chr2.3:70329873-70329892 | None:intergenic | 45.0% | |
| CAAAAAACGCCAATTGCCAC+AGG | - | chr2.3:70330043-70330062 | None:intergenic | 45.0% | |
| CCAATTCCATGTCTCTCAGT+AGG | - | chr2.3:70330088-70330107 | None:intergenic | 45.0% | |
| CCTACTGAGAGACATGGAAT+TGG | + | chr2.3:70330085-70330104 | MS.gene047359:CDS | 45.0% | |
| GCATTTCCTACTGAGAGACA+TGG | + | chr2.3:70330079-70330098 | MS.gene047359:CDS | 45.0% | |
| TATGGGTTTGCCACCTTCAA+AGG | + | chr2.3:70329824-70329843 | MS.gene047359:CDS | 45.0% | |
| TGCCACCTTCAAAGGTTTGT+GGG | + | chr2.3:70329832-70329851 | MS.gene047359:CDS | 45.0% | |
| TTGCCACCTTCAAAGGTTTG+TGG | + | chr2.3:70329831-70329850 | MS.gene047359:CDS | 45.0% | |
| TTGGCAAACCTTCTACCTAC+AGG | + | chr2.3:70329638-70329657 | MS.gene047359:CDS | 45.0% | |
| ATTTCAGAGCACGGCGCATT+TGG | + | chr2.3:70329619-70329638 | MS.gene047359:CDS | 50.0% | |
| GAACAGTGCCTGTAGGTAGA+AGG | - | chr2.3:70329649-70329668 | None:intergenic | 50.0% | |
| GCATGCGGTGATGTCAATTC+TGG | + | chr2.3:70329925-70329944 | MS.gene047359:CDS | 50.0% | |
| ! | GACCCACAAACCTTTGAAGG+TGG | - | chr2.3:70329837-70329856 | None:intergenic | 50.0% |
| GGTTCCACTTCTGCGGAATG+CGG | + | chr2.3:70329547-70329566 | MS.gene047359:CDS | 55.0% | |
| AATCCTCACGGCGTTGCCTG+TGG | + | chr2.3:70330024-70330043 | MS.gene047359:CDS | 60.0% | |
| ACCTCCGCATTCCGCAGAAG+TGG | - | chr2.3:70329554-70329573 | None:intergenic | 60.0% | |
| ACGGCGTTGCCTGTGGCAAT+TGG | + | chr2.3:70330031-70330050 | MS.gene047359:CDS | 60.0% | |
| TCCACTTCTGCGGAATGCGG+AGG | + | chr2.3:70329550-70329569 | MS.gene047359:CDS | 60.0% | |
| ACCAGGCTCCTCTCTGCCTC+TGG | - | chr2.3:70329578-70329597 | None:intergenic | 65.0% | |
| CGGAGGTTCCAGAGGCAGAG+AGG | + | chr2.3:70329567-70329586 | MS.gene047359:CDS | 65.0% | |
| GCGGAATGCGGAGGTTCCAG+AGG | + | chr2.3:70329559-70329578 | MS.gene047359:CDS | 65.0% | |
| TCCAGAGGCAGAGAGGAGCC+TGG | + | chr2.3:70329574-70329593 | MS.gene047359:CDS | 65.0% | |
| TTGCCACAGGCAACGCCGTG+AGG | - | chr2.3:70330030-70330049 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 70329494 | 70330129 | 70329494 | ID=MS.gene047359 |
| chr2.3 | mRNA | 70329494 | 70330129 | 70329494 | ID=MS.gene047359.t1;Parent=MS.gene047359 |
| chr2.3 | exon | 70329494 | 70330129 | 70329494 | ID=MS.gene047359.t1.exon1;Parent=MS.gene047359.t1 |
| chr2.3 | CDS | 70329494 | 70330129 | 70329494 | ID=cds.MS.gene047359.t1;Parent=MS.gene047359.t1 |
| Gene Sequence |
| Protein sequence |
