Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43093.t1 | XP_003593648.1 | 98.6 | 211 | 2 | 1 | 1 | 211 | 1 | 210 | 3.70E-109 | 404.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43093.t1 | Q9FNL3 | 61.7 | 214 | 75 | 3 | 1 | 209 | 1 | 212 | 5.3e-72 | 272.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43093.t1 | A2Q514 | 98.6 | 211 | 2 | 1 | 1 | 211 | 1 | 210 | 2.7e-109 | 404.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43093 | MS.gene047221 | PPI |
| MS.gene43093 | MS.gene072079 | PPI |
| MS.gene43093 | MS.gene002025 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43093.t1 | MTR_2g014550 | 98.578 | 211 | 2 | 1 | 1 | 211 | 1 | 210 | 3.37e-152 | 422 |
| MS.gene43093.t1 | MTR_2g014520 | 88.406 | 207 | 24 | 0 | 5 | 211 | 1 | 207 | 6.18e-135 | 379 |
| MS.gene43093.t1 | MTR_4g088335 | 73.604 | 197 | 52 | 0 | 15 | 211 | 10 | 206 | 2.50e-109 | 313 |
| MS.gene43093.t1 | MTR_4g078870 | 54.922 | 193 | 81 | 3 | 20 | 209 | 14 | 203 | 5.63e-75 | 226 |
| MS.gene43093.t1 | MTR_5g044580 | 43.094 | 181 | 101 | 2 | 26 | 205 | 35 | 214 | 1.73e-48 | 159 |
| MS.gene43093.t1 | MTR_2g020850 | 38.947 | 190 | 109 | 2 | 25 | 210 | 2 | 188 | 8.54e-46 | 152 |
| MS.gene43093.t1 | MTR_7g010890 | 41.436 | 181 | 104 | 2 | 26 | 205 | 35 | 214 | 1.90e-45 | 152 |
| MS.gene43093.t1 | MTR_4g118470 | 41.885 | 191 | 97 | 3 | 24 | 210 | 18 | 198 | 6.23e-43 | 145 |
| MS.gene43093.t1 | MTR_4g118480 | 39.512 | 205 | 110 | 3 | 10 | 210 | 2 | 196 | 7.23e-41 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43093.t1 | AT5G46090 | 61.682 | 214 | 75 | 3 | 1 | 209 | 1 | 212 | 2.99e-97 | 283 |
| MS.gene43093.t1 | AT4G18425 | 59.434 | 212 | 82 | 3 | 3 | 210 | 1 | 212 | 5.39e-92 | 270 |
| MS.gene43093.t1 | AT4G28485 | 51.759 | 199 | 90 | 3 | 15 | 209 | 2 | 198 | 2.23e-66 | 204 |
| MS.gene43093.t1 | AT3G02430 | 47.644 | 191 | 94 | 2 | 25 | 211 | 27 | 215 | 4.08e-64 | 199 |
| MS.gene43093.t1 | AT4G24310 | 50.000 | 192 | 89 | 3 | 23 | 209 | 18 | 207 | 1.40e-60 | 190 |
| MS.gene43093.t1 | AT1G09157 | 38.587 | 184 | 101 | 1 | 34 | 205 | 56 | 239 | 6.91e-42 | 143 |
| MS.gene43093.t1 | AT3G21550 | 43.373 | 166 | 89 | 1 | 42 | 207 | 12 | 172 | 1.32e-41 | 140 |
| MS.gene43093.t1 | AT5G39650 | 38.172 | 186 | 99 | 2 | 34 | 205 | 57 | 240 | 5.00e-41 | 141 |
| MS.gene43093.t1 | AT3G21520 | 40.000 | 160 | 89 | 2 | 49 | 206 | 33 | 187 | 1.78e-35 | 125 |
| MS.gene43093.t1 | AT4G28485 | 57.576 | 99 | 42 | 0 | 111 | 209 | 65 | 163 | 2.55e-33 | 119 |
| MS.gene43093.t1 | AT5G27370 | 35.227 | 176 | 99 | 3 | 45 | 205 | 11 | 186 | 8.69e-30 | 110 |
Find 54 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCAGAGCCTTTATGATCTT+TGG | 0.202160 | 7.2:-76885867 | None:intergenic |
| ATGATCTTTGGGTCTCTTGT+AGG | 0.235662 | 7.2:-76885855 | None:intergenic |
| ACCAGGCTCCTCTCTGCTTC+TGG | 0.261889 | 7.2:-76885136 | None:intergenic |
| TAAAGGCTCTGACCCTTATC+TGG | 0.306109 | 7.2:+76885877 | MS.gene43093:CDS |
| TCAGAGCCTTTATGATCTTT+GGG | 0.314421 | 7.2:-76885866 | None:intergenic |
| CCCTTATCTGGACCTACTAT+TGG | 0.331346 | 7.2:+76885889 | MS.gene43093:CDS |
| AAGGGCTTGTGGGTTATTGA+TGG | 0.336018 | 7.2:+76885403 | MS.gene43093:CDS |
| GAACAGTGCCCGTAGGTAAA+AGG | 0.336321 | 7.2:-76885207 | None:intergenic |
| TTATGGGTTTGCCACCTTCA+AGG | 0.356644 | 7.2:+76885384 | MS.gene43093:CDS |
| AGCAAGGGGAATATTTGTTA+TGG | 0.356880 | 7.2:+76885367 | MS.gene43093:CDS |
| GCAAGGGGAATATTTGTTAT+GGG | 0.374957 | 7.2:+76885368 | MS.gene43093:CDS |
| ACAGCGTTGCCTGTGGCAAT+TGG | 0.386333 | 7.2:+76885592 | MS.gene43093:CDS |
| ATTTCAGAGCACGGCACATT+TGG | 0.405380 | 7.2:+76885180 | MS.gene43093:CDS |
| TGCCACCTTCAAGGGCTTGT+GGG | 0.408909 | 7.2:+76885393 | MS.gene43093:CDS |
| CCTACTGAGAGACATGGAAT+TGG | 0.411080 | 7.2:+76885646 | MS.gene43093:CDS |
| TATGGGTTTGCCACCTTCAA+GGG | 0.411112 | 7.2:+76885385 | MS.gene43093:CDS |
| CAATTATTAACTTGTTGAAA+GGG | 0.415154 | 7.2:-76885674 | None:intergenic |
| AGCAACTTGAGGTGGAAGTT+TGG | 0.423290 | 7.2:-76885431 | None:intergenic |
| TTGCCACCTTCAAGGGCTTG+TGG | 0.427940 | 7.2:+76885392 | MS.gene43093:CDS |
| AACTTATAGCTTTCTGTACC+AGG | 0.430636 | 7.2:-76885153 | None:intergenic |
| GCATGCGGTGATGTCAATTC+TGG | 0.434994 | 7.2:+76885486 | MS.gene43093:CDS |
| TGATCTTTGGGTCTCTTGTA+GGG | 0.435922 | 7.2:-76885854 | None:intergenic |
| AAGAGACCCAAAGATCATAA+AGG | 0.455510 | 7.2:+76885860 | MS.gene43093:CDS |
| TGCTATCAGTAAAGTTTAGC+AGG | 0.458079 | 7.2:-76885336 | None:intergenic |
| TCAATTATTAACTTGTTGAA+AGG | 0.463534 | 7.2:-76885675 | None:intergenic |
| TAAGAATAACGATGAACAAA+AGG | 0.479910 | 7.2:+76885087 | MS.gene43093:CDS |
| TCCAATAGTAGGTCCAGATA+AGG | 0.496452 | 7.2:-76885890 | None:intergenic |
| ATTTACGAAGTTCCAATAGT+AGG | 0.505101 | 7.2:-76885901 | None:intergenic |
| CTGGAACCTCCGCATTCCGC+AGG | 0.507308 | 7.2:-76885117 | None:intergenic |
| CCAATAGTAGGTCCAGATAA+GGG | 0.518295 | 7.2:-76885889 | None:intergenic |
| AATAACCCACAAGCCCTTGA+AGG | 0.520322 | 7.2:-76885398 | None:intergenic |
| AATTATTAACTTGTTGAAAG+GGG | 0.546060 | 7.2:-76885673 | None:intergenic |
| TCTGTACTTGTGACTCTATG+TGG | 0.546587 | 7.2:+76885301 | MS.gene43093:CDS |
| TCCAGAAGCAGAGAGGAGCC+TGG | 0.549208 | 7.2:+76885135 | MS.gene43093:CDS |
| CCAATTCCATGTCTCTCAGT+AGG | 0.555703 | 7.2:-76885646 | None:intergenic |
| AGTCACAAGTACAGAAGTCA+TGG | 0.564650 | 7.2:-76885293 | None:intergenic |
| ACCTCCGCATTCCGCAGGAG+TGG | 0.568210 | 7.2:-76885112 | None:intergenic |
| TGATAGCATTAGAGACAGCA+AGG | 0.586072 | 7.2:+76885351 | MS.gene43093:CDS |
| GTTTATAGATTTCATGCATG+CGG | 0.586781 | 7.2:+76885471 | MS.gene43093:CDS |
| ATTATTAACTTGTTGAAAGG+GGG | 0.590301 | 7.2:-76885672 | None:intergenic |
| AAGTTCGACATTTCAGAGCA+CGG | 0.594731 | 7.2:+76885171 | MS.gene43093:CDS |
| TCCACTCCTGCGGAATGCGG+AGG | 0.597748 | 7.2:+76885111 | MS.gene43093:CDS |
| TTGCCACAGGCAACGCTGTG+AGG | 0.604875 | 7.2:-76885588 | None:intergenic |
| AACAAAAGGTTCCACTCCTG+CGG | 0.611057 | 7.2:+76885101 | MS.gene43093:CDS |
| GGTTCCACTCCTGCGGAATG+CGG | 0.623916 | 7.2:+76885108 | MS.gene43093:CDS |
| AGAAGTCATGGACTTGCTGA+CGG | 0.626924 | 7.2:-76885281 | None:intergenic |
| TGCATTGTTTGATCAGAATG+TGG | 0.627363 | 7.2:+76885522 | MS.gene43093:CDS |
| AACCCACAAGCCCTTGAAGG+TGG | 0.635020 | 7.2:-76885395 | None:intergenic |
| GATAGCATTAGAGACAGCAA+GGG | 0.637719 | 7.2:+76885352 | MS.gene43093:CDS |
| GCATTTCCTACTGAGAGACA+TGG | 0.643869 | 7.2:+76885640 | MS.gene43093:CDS |
| CGGAGGTTCCAGAAGCAGAG+AGG | 0.659785 | 7.2:+76885128 | MS.gene43093:CDS |
| AATCCTCACAGCGTTGCCTG+TGG | 0.661593 | 7.2:+76885585 | MS.gene43093:CDS |
| AAAGCAAGAACAGTGCCCGT+AGG | 0.697440 | 7.2:-76885214 | None:intergenic |
| ATAGCATTAGAGACAGCAAG+GGG | 0.732552 | 7.2:+76885353 | MS.gene43093:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTATTTGATTAATGAATTAT+TGG | + | chr7.2:76885738-76885757 | MS.gene43093:intron | 15.0% |
| !! | AATGAGAATAAATTGAAACA+TGG | - | chr7.2:76885934-76885953 | None:intergenic | 20.0% |
| !! | ATCAAATACATTACTGAAAT+CGG | - | chr7.2:76885727-76885746 | None:intergenic | 20.0% |
| !!! | AATTATTAACTTGTTGAAAG+GGG | - | chr7.2:76885676-76885695 | None:intergenic | 20.0% |
| !!! | CAATTATTAACTTGTTGAAA+GGG | - | chr7.2:76885677-76885696 | None:intergenic | 20.0% |
| !!! | TCAATTATTAACTTGTTGAA+AGG | - | chr7.2:76885678-76885697 | None:intergenic | 20.0% |
| !!! | TGAGATCTTTTTTATGTTTA+TGG | + | chr7.2:76885695-76885714 | MS.gene43093:intron | 20.0% |
| !!! | TTTTAAATTGCAATACTACA+AGG | + | chr7.2:76885824-76885843 | MS.gene43093:intron | 20.0% |
| ! | TAAGAATAACGATGAACAAA+AGG | + | chr7.2:76885087-76885106 | MS.gene43093:CDS | 25.0% |
| ! | TTGCCAACATTTGTAAAAAT+TGG | - | chr7.2:76885253-76885272 | None:intergenic | 25.0% |
| !! | ATTTCTTGTATCTCTTTTGA+TGG | - | chr7.2:76885568-76885587 | None:intergenic | 25.0% |
| !! | TAAAAATTGGTGACAAAAGT+TGG | - | chr7.2:76885240-76885259 | None:intergenic | 25.0% |
| !! | TATTGGCGTTTTTATGTTTA+TGG | + | chr7.2:76885755-76885774 | MS.gene43093:intron | 25.0% |
| !! | TCACCAATTTTTACAAATGT+TGG | + | chr7.2:76885247-76885266 | MS.gene43093:CDS | 25.0% |
| !!! | ATTATTAACTTGTTGAAAGG+GGG | - | chr7.2:76885675-76885694 | None:intergenic | 25.0% |
| ATTTACGAAGTTCCAATAGT+AGG | - | chr7.2:76885904-76885923 | None:intergenic | 30.0% | |
| GTTTATAGATTTCATGCATG+CGG | + | chr7.2:76885471-76885490 | MS.gene43093:CDS | 30.0% | |
| !! | TGTGGTTTTTCTTTTTCACT+AGG | + | chr7.2:76885983-76886002 | MS.gene43093:intron | 30.0% |
| AACTTATAGCTTTCTGTACC+AGG | - | chr7.2:76885156-76885175 | None:intergenic | 35.0% | |
| AAGAGACCCAAAGATCATAA+AGG | + | chr7.2:76885860-76885879 | MS.gene43093:CDS | 35.0% | |
| TCAGAGCCTTTATGATCTTT+GGG | - | chr7.2:76885869-76885888 | None:intergenic | 35.0% | |
| TGCATTGTTTGATCAGAATG+TGG | + | chr7.2:76885522-76885541 | MS.gene43093:CDS | 35.0% | |
| TGCTATCAGTAAAGTTTAGC+AGG | - | chr7.2:76885339-76885358 | None:intergenic | 35.0% | |
| TTCTTCTTCACTCTATTGTG+TGG | + | chr7.2:76885965-76885984 | MS.gene43093:intron | 35.0% | |
| ! | AGCAAGGGGAATATTTGTTA+TGG | + | chr7.2:76885367-76885386 | MS.gene43093:CDS | 35.0% |
| ! | TTTGGCAAACCTTTTACCTA+CGG | + | chr7.2:76885198-76885217 | MS.gene43093:CDS | 35.0% |
| !! | GCAAGGGGAATATTTGTTAT+GGG | + | chr7.2:76885368-76885387 | MS.gene43093:CDS | 35.0% |
| !! | TGTATCTCTTTTGATGGTTC+TGG | - | chr7.2:76885562-76885581 | None:intergenic | 35.0% |
| AAGTTCGACATTTCAGAGCA+CGG | + | chr7.2:76885171-76885190 | MS.gene43093:CDS | 40.0% | |
| AGTCACAAGTACAGAAGTCA+TGG | - | chr7.2:76885296-76885315 | None:intergenic | 40.0% | |
| ATAGCATTAGAGACAGCAAG+GGG | + | chr7.2:76885353-76885372 | MS.gene43093:CDS | 40.0% | |
| ATGATCTTTGGGTCTCTTGT+AGG | - | chr7.2:76885858-76885877 | None:intergenic | 40.0% | |
| CCAATAGTAGGTCCAGATAA+GGG | - | chr7.2:76885892-76885911 | None:intergenic | 40.0% | |
| GATAGCATTAGAGACAGCAA+GGG | + | chr7.2:76885352-76885371 | MS.gene43093:CDS | 40.0% | |
| GTCAGAGCCTTTATGATCTT+TGG | - | chr7.2:76885870-76885889 | None:intergenic | 40.0% | |
| TCCAATAGTAGGTCCAGATA+AGG | - | chr7.2:76885893-76885912 | None:intergenic | 40.0% | |
| TCTGTACTTGTGACTCTATG+TGG | + | chr7.2:76885301-76885320 | MS.gene43093:CDS | 40.0% | |
| TGATAGCATTAGAGACAGCA+AGG | + | chr7.2:76885351-76885370 | MS.gene43093:CDS | 40.0% | |
| TGATCTTTGGGTCTCTTGTA+GGG | - | chr7.2:76885857-76885876 | None:intergenic | 40.0% | |
| ! | CGATATTTTGCAGCAACTTG+AGG | - | chr7.2:76885445-76885464 | None:intergenic | 40.0% |
| ! | TATTTTGCAGCAACTTGAGG+TGG | - | chr7.2:76885442-76885461 | None:intergenic | 40.0% |
| ! | TTGGCAAACCTTTTACCTAC+GGG | + | chr7.2:76885199-76885218 | MS.gene43093:CDS | 40.0% |
| AACAAAAGGTTCCACTCCTG+CGG | + | chr7.2:76885101-76885120 | MS.gene43093:CDS | 45.0% | |
| AATAACCCACAAGCCCTTGA+AGG | - | chr7.2:76885401-76885420 | None:intergenic | 45.0% | |
| AGCAACTTGAGGTGGAAGTT+TGG | - | chr7.2:76885434-76885453 | None:intergenic | 45.0% | |
| ATTTCAGAGCACGGCACATT+TGG | + | chr7.2:76885180-76885199 | MS.gene43093:CDS | 45.0% | |
| CAAAAAACGCCAATTGCCAC+AGG | - | chr7.2:76885604-76885623 | None:intergenic | 45.0% | |
| CCAATTCCATGTCTCTCAGT+AGG | - | chr7.2:76885649-76885668 | None:intergenic | 45.0% | |
| CCCTTATCTGGACCTACTAT+TGG | + | chr7.2:76885889-76885908 | MS.gene43093:CDS | 45.0% | |
| CCTACTGAGAGACATGGAAT+TGG | + | chr7.2:76885646-76885665 | MS.gene43093:CDS | 45.0% | |
| GCATTTCCTACTGAGAGACA+TGG | + | chr7.2:76885640-76885659 | MS.gene43093:CDS | 45.0% | |
| TATGGGTTTGCCACCTTCAA+GGG | + | chr7.2:76885385-76885404 | MS.gene43093:CDS | 45.0% | |
| TTATGGGTTTGCCACCTTCA+AGG | + | chr7.2:76885384-76885403 | MS.gene43093:CDS | 45.0% | |
| ! | AAGGGCTTGTGGGTTATTGA+TGG | + | chr7.2:76885403-76885422 | MS.gene43093:CDS | 45.0% |
| ! | TAAAGGCTCTGACCCTTATC+TGG | + | chr7.2:76885877-76885896 | MS.gene43093:CDS | 45.0% |
| !! | AGAAGTCATGGACTTGCTGA+CGG | - | chr7.2:76885284-76885303 | None:intergenic | 45.0% |
| AAAGCAAGAACAGTGCCCGT+AGG | - | chr7.2:76885217-76885236 | None:intergenic | 50.0% | |
| GAACAGTGCCCGTAGGTAAA+AGG | - | chr7.2:76885210-76885229 | None:intergenic | 50.0% | |
| GCATGCGGTGATGTCAATTC+TGG | + | chr7.2:76885486-76885505 | MS.gene43093:CDS | 50.0% | |
| AACCCACAAGCCCTTGAAGG+TGG | - | chr7.2:76885398-76885417 | None:intergenic | 55.0% | |
| AATCCTCACAGCGTTGCCTG+TGG | + | chr7.2:76885585-76885604 | MS.gene43093:CDS | 55.0% | |
| ACAGCGTTGCCTGTGGCAAT+TGG | + | chr7.2:76885592-76885611 | MS.gene43093:CDS | 55.0% | |
| TGCCACCTTCAAGGGCTTGT+GGG | + | chr7.2:76885393-76885412 | MS.gene43093:CDS | 55.0% | |
| TTGCCACCTTCAAGGGCTTG+TGG | + | chr7.2:76885392-76885411 | MS.gene43093:CDS | 55.0% | |
| ACCAGGCTCCTCTCTGCTTC+TGG | - | chr7.2:76885139-76885158 | None:intergenic | 60.0% | |
| CGGAGGTTCCAGAAGCAGAG+AGG | + | chr7.2:76885128-76885147 | MS.gene43093:CDS | 60.0% | |
| GGTTCCACTCCTGCGGAATG+CGG | + | chr7.2:76885108-76885127 | MS.gene43093:CDS | 60.0% | |
| TCCAGAAGCAGAGAGGAGCC+TGG | + | chr7.2:76885135-76885154 | MS.gene43093:CDS | 60.0% | |
| TTGCCACAGGCAACGCTGTG+AGG | - | chr7.2:76885591-76885610 | None:intergenic | 60.0% | |
| ACCTCCGCATTCCGCAGGAG+TGG | - | chr7.2:76885115-76885134 | None:intergenic | 65.0% | |
| CTGGAACCTCCGCATTCCGC+AGG | - | chr7.2:76885120-76885139 | None:intergenic | 65.0% | |
| TCCACTCCTGCGGAATGCGG+AGG | + | chr7.2:76885111-76885130 | MS.gene43093:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 76885055 | 76886047 | 76885055 | ID=MS.gene43093 |
| chr7.2 | mRNA | 76885055 | 76886047 | 76885055 | ID=MS.gene43093.t1;Parent=MS.gene43093 |
| chr7.2 | exon | 76885055 | 76885685 | 76885055 | ID=MS.gene43093.t1.exon1;Parent=MS.gene43093.t1 |
| chr7.2 | CDS | 76885055 | 76885685 | 76885055 | ID=cds.MS.gene43093.t1;Parent=MS.gene43093.t1 |
| chr7.2 | exon | 76885846 | 76885924 | 76885846 | ID=MS.gene43093.t1.exon2;Parent=MS.gene43093.t1 |
| chr7.2 | CDS | 76885846 | 76885924 | 76885846 | ID=cds.MS.gene43093.t1;Parent=MS.gene43093.t1 |
| chr7.2 | exon | 76886005 | 76886047 | 76886005 | ID=MS.gene43093.t1.exon3;Parent=MS.gene43093.t1 |
| chr7.2 | CDS | 76886005 | 76886047 | 76886005 | ID=cds.MS.gene43093.t1;Parent=MS.gene43093.t1 |
| Gene Sequence |
| Protein sequence |
