AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00274.t1 MTR_2g435930 98.028 507 10 0 1 507 1 507 0.0 1009
MS.gene00274.t1 MTR_6g043810 46.386 498 251 3 11 507 15 497 3.44e-160 464
MS.gene00274.t1 MTR_6g043810 46.386 498 251 3 11 507 25 507 4.91e-160 464
MS.gene00274.t1 MTR_3g111020 46.875 480 239 2 2 481 34 497 3.31e-152 446
MS.gene00274.t1 MTR_5g067460 46.186 472 236 3 11 481 47 501 9.46e-148 435
MS.gene00274.t1 MTR_0039s0080 43.359 512 259 4 1 507 17 502 9.28e-146 428
MS.gene00274.t1 MTR_1g101500 44.554 505 240 5 8 497 55 534 1.65e-141 418
MS.gene00274.t1 MTR_5g010830 43.856 472 247 3 5 476 53 506 6.58e-135 401
MS.gene00274.t1 MTR_8g102430 42.918 473 231 6 7 476 8 444 6.12e-132 390
MS.gene00274.t1 MTR_6g088805 42.308 468 251 5 17 484 25 473 7.48e-131 389
MS.gene00274.t1 MTR_4g027920 40.725 469 264 2 13 481 150 604 6.08e-121 368
MS.gene00274.t1 MTR_5g011540 32.914 477 305 3 4 480 28 489 3.75e-81 261
MS.gene00274.t1 MTR_6g081410 28.601 479 328 2 1 479 7 471 1.14e-74 244
MS.gene00274.t1 MTR_6g081810 29.387 473 292 3 5 477 13 443 5.48e-73 238
MS.gene00274.t1 MTR_6g081240 31.441 458 290 6 16 469 7 444 3.51e-71 234
MS.gene00274.t1 MTR_6g081400 31.681 464 298 4 16 477 12 458 3.96e-69 229
MS.gene00274.t1 MTR_6g081780 27.010 485 328 3 1 479 7 471 1.13e-68 228
MS.gene00274.t1 MTR_6g081300 30.290 482 309 5 16 492 7 466 1.52e-68 227
MS.gene00274.t1 MTR_6g066380 28.601 479 326 3 1 477 7 471 5.31e-68 226
MS.gene00274.t1 MTR_8g106670 27.463 477 330 2 17 493 10 470 1.01e-65 220
MS.gene00274.t1 MTR_6g004220 29.512 471 315 3 8 477 12 466 1.84e-65 219
MS.gene00274.t1 MTR_1g108810 29.825 456 300 5 16 469 31 468 5.61e-65 218
MS.gene00274.t1 MTR_1g108840 28.602 472 320 3 4 474 25 480 1.77e-64 217
MS.gene00274.t1 MTR_6g004220 29.590 463 309 3 8 469 19 465 1.57e-63 215
MS.gene00274.t1 MTR_6g081310 30.655 473 280 6 16 473 7 446 2.18e-63 214
MS.gene00274.t1 MTR_6g081390 29.957 464 306 4 16 477 9 455 9.90e-62 209
MS.gene00274.t1 MTR_7g082810 29.025 472 313 5 13 481 14 466 3.41e-60 206
MS.gene00274.t1 MTR_1g109060 28.633 461 309 4 19 477 24 466 2.98e-57 197
MS.gene00274.t1 MTR_7g082800 31.017 403 262 2 78 480 1 387 8.55e-56 192
MS.gene00274.t1 MTR_1g109060 28.698 453 303 4 19 469 24 458 6.45e-55 192
MS.gene00274.t1 MTR_1g108990 27.586 464 318 2 19 481 25 471 8.82e-51 180
MS.gene00274.t1 MTR_2g078930 26.362 459 320 3 18 475 42 483 6.89e-49 176
MS.gene00274.t1 MTR_6g004220 29.058 382 254 3 8 388 19 384 3.25e-48 173
MS.gene00274.t1 MTR_8g069470 27.049 488 313 11 6 477 31 491 2.31e-45 166
MS.gene00274.t1 MTR_2g079020 25.862 464 324 4 18 480 36 480 7.58e-45 165
MS.gene00274.t1 MTR_2g078960 25.974 462 326 3 18 479 32 477 2.04e-44 163
MS.gene00274.t1 MTR_4g048000 24.236 458 325 3 20 474 65 503 2.71e-41 155
MS.gene00274.t1 MTR_5g033100 24.249 466 332 5 16 479 53 499 4.84e-41 154
MS.gene00274.t1 MTR_2g022980 24.401 459 319 5 25 475 50 488 5.29e-41 154
MS.gene00274.t1 MTR_2g088400 24.508 457 323 3 20 473 53 490 7.29e-41 154
MS.gene00274.t1 MTR_2g078970 24.837 459 327 3 18 475 30 471 1.36e-40 153
MS.gene00274.t1 MTR_6g047610 26.134 463 299 7 19 469 31 462 3.79e-39 149
MS.gene00274.t1 MTR_5g032720 25.806 465 321 7 14 474 31 475 4.72e-38 145
MS.gene00274.t1 MTR_1g021740 23.270 477 347 3 2 477 18 476 6.33e-38 145
MS.gene00274.t1 MTR_5g032750 25.541 462 322 7 16 474 33 475 5.55e-37 142
MS.gene00274.t1 MTR_6g004220 29.073 313 205 3 8 319 19 315 1.24e-36 138
MS.gene00274.t1 MTR_2g088400 24.533 428 300 4 20 444 53 460 7.09e-36 139
MS.gene00274.t1 MTR_8g063590 26.328 433 302 5 50 481 84 500 1.23e-35 139
MS.gene00274.t1 MTR_5g090290 25.745 470 322 7 31 492 54 504 6.49e-34 135
MS.gene00274.t1 MTR_1g100180 24.612 451 320 4 20 469 44 475 1.87e-33 133
MS.gene00274.t1 MTR_3g099720 22.453 481 356 4 4 484 26 489 5.98e-33 131
MS.gene00274.t1 MTR_7g087410 24.256 437 302 4 49 479 61 474 1.13e-32 130
MS.gene00274.t1 MTR_7g087430 24.570 407 276 3 78 477 1 383 6.86e-32 127
MS.gene00274.t1 MTR_3g099700 24.109 477 340 6 4 477 31 488 1.63e-31 127
MS.gene00274.t1 MTR_7g087400 22.318 466 336 4 16 477 27 470 3.00e-31 126
MS.gene00274.t1 MTR_6g047640 23.789 454 303 4 19 469 31 444 2.43e-30 123
MS.gene00274.t1 MTR_6g047640 24.449 454 319 3 19 469 31 463 8.34e-30 122
MS.gene00274.t1 MTR_7g087410 23.153 406 287 2 78 479 1 385 1.93e-29 120
MS.gene00274.t1 MTR_6g047590 25.442 452 316 6 19 469 31 462 7.64e-29 119
MS.gene00274.t1 MTR_7g087370 21.684 475 317 5 7 477 18 441 6.94e-26 110
MS.gene00274.t1 MTR_6g027200 22.465 503 353 6 3 490 20 500 3.63e-24 105
MS.gene00274.t1 MTR_5g087930 22.538 457 309 9 34 483 41 459 2.18e-21 97.4
MS.gene00274.t1 MTR_6g027190 22.718 471 329 8 8 469 13 457 5.38e-20 93.2
MS.gene00274.t1 MTR_2g078990 24.766 214 161 0 262 475 87 300 4.13e-17 82.8
MS.gene00274.t1 MTR_2g079030 26.923 208 148 3 274 480 319 523 3.60e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00274.t1 AT5G49130 62.427 511 171 5 1 500 1 501 0.0 618
MS.gene00274.t1 AT1G71870 46.108 501 259 4 3 493 7 506 6.38e-155 451
MS.gene00274.t1 AT4G29140 43.902 492 253 3 4 495 40 508 1.11e-144 426
MS.gene00274.t1 AT5G19700 43.408 493 255 6 4 492 20 492 1.56e-137 407
MS.gene00274.t1 AT5G52050 41.970 467 261 2 18 484 33 489 2.91e-134 398
MS.gene00274.t1 AT1G58340 44.374 471 246 3 9 479 47 501 1.95e-133 397
MS.gene00274.t1 AT4G23030 43.265 490 243 5 5 483 14 479 5.58e-121 364
MS.gene00274.t1 AT2G38510 37.323 493 290 5 16 507 2 476 1.75e-118 357
MS.gene00274.t1 AT4G22790 33.400 503 300 6 1 492 1 479 4.34e-78 253
MS.gene00274.t1 AT5G52450 30.041 486 320 4 10 492 13 481 5.82e-67 224
MS.gene00274.t1 AT1G64820 30.691 492 316 4 18 505 27 497 2.44e-65 220
MS.gene00274.t1 AT1G15150 29.913 458 301 3 20 475 31 470 5.40e-64 216
MS.gene00274.t1 AT1G15170 30.263 456 302 2 20 475 34 473 1.40e-63 214
MS.gene00274.t1 AT2G34360 28.169 497 322 6 1 487 1 472 5.08e-61 208
MS.gene00274.t1 AT1G66760 29.437 462 310 3 19 480 27 472 7.66e-61 207
MS.gene00274.t1 AT1G71140 29.247 465 309 4 18 480 24 470 1.01e-60 207
MS.gene00274.t1 AT2G04080 28.992 476 318 4 4 477 11 468 1.39e-60 206
MS.gene00274.t1 AT2G04100 28.390 472 321 3 7 477 16 471 4.71e-60 205
MS.gene00274.t1 AT1G15160 29.847 459 298 5 20 474 31 469 7.20e-60 205
MS.gene00274.t1 AT2G04070 29.451 455 305 2 20 474 27 465 2.74e-59 203
MS.gene00274.t1 AT1G15180 31.659 458 293 4 20 475 35 474 2.83e-59 203
MS.gene00274.t1 AT2G04050 31.102 463 299 4 14 474 21 465 6.79e-59 202
MS.gene00274.t1 AT2G04080 29.060 468 312 4 4 469 11 460 7.60e-59 202
MS.gene00274.t1 AT2G04040 29.299 471 316 3 7 477 15 468 7.61e-59 202
MS.gene00274.t1 AT2G04100 28.448 464 315 3 7 469 16 463 2.16e-58 201
MS.gene00274.t1 AT1G73700 28.846 468 317 2 13 480 17 468 2.43e-57 198
MS.gene00274.t1 AT1G66760 29.490 451 302 3 19 469 27 461 5.69e-57 197
MS.gene00274.t1 AT1G66780 28.903 474 318 3 4 477 22 476 4.62e-56 195
MS.gene00274.t1 AT2G04090 26.819 481 331 5 6 483 15 477 9.76e-55 191
MS.gene00274.t1 AT1G15170 29.973 367 241 2 109 475 4 354 1.07e-51 180
MS.gene00274.t1 AT1G11670 25.934 455 321 4 20 474 50 488 3.43e-50 179
MS.gene00274.t1 AT1G61890 25.793 473 331 5 7 475 30 486 2.24e-48 174
MS.gene00274.t1 AT1G61890 25.482 467 328 5 7 469 30 480 4.48e-47 171
MS.gene00274.t1 AT1G15150 29.396 364 237 3 114 475 9 354 3.15e-46 166
MS.gene00274.t1 AT3G23550 27.093 454 309 3 18 468 29 463 7.57e-44 161
MS.gene00274.t1 AT1G66760 29.620 368 243 3 19 386 27 378 1.21e-43 159
MS.gene00274.t1 AT3G21690 25.490 459 318 4 20 474 52 490 5.73e-42 157
MS.gene00274.t1 AT1G61890 25.688 436 304 5 7 438 30 449 6.77e-42 156
MS.gene00274.t1 AT4G21910 25.714 455 321 5 20 474 55 492 8.26e-42 156
MS.gene00274.t1 AT4G21910 25.714 455 321 5 20 474 55 492 8.26e-42 156
MS.gene00274.t1 AT3G59030 26.829 451 312 6 34 483 65 498 1.11e-41 156
MS.gene00274.t1 AT3G26590 24.729 461 320 7 20 475 45 483 2.32e-41 155
MS.gene00274.t1 AT4G21903 26.047 430 291 6 50 474 83 490 4.85e-41 154
MS.gene00274.t1 AT4G21910 25.000 456 325 5 20 475 57 495 3.90e-40 152
MS.gene00274.t1 AT4G21910 25.000 456 325 5 20 475 57 495 3.90e-40 152
MS.gene00274.t1 AT4G21903 25.676 444 299 7 50 484 83 504 4.33e-40 152
MS.gene00274.t1 AT5G38030 24.473 474 328 8 20 485 45 496 4.80e-40 151
MS.gene00274.t1 AT3G23560 25.771 454 315 2 18 468 37 471 6.07e-39 148
MS.gene00274.t1 AT4G21903 25.377 398 273 4 81 474 4 381 1.76e-38 145
MS.gene00274.t1 AT3G26590 24.623 398 277 5 81 475 4 381 2.90e-38 144
MS.gene00274.t1 AT1G12950 24.464 466 319 8 20 477 67 507 5.25e-38 146
MS.gene00274.t1 AT4G00350 25.054 459 324 4 18 475 86 525 6.15e-38 146
MS.gene00274.t1 AT5G44050 25.208 480 326 8 2 474 25 478 2.83e-37 144
MS.gene00274.t1 AT1G47530 23.605 466 331 5 16 477 31 475 1.98e-35 138
MS.gene00274.t1 AT1G66760 27.666 347 235 3 123 469 3 333 3.61e-35 134
MS.gene00274.t1 AT1G23300 24.848 495 330 8 16 499 40 503 4.98e-34 135
MS.gene00274.t1 AT1G15180 30.496 282 176 4 109 388 4 267 7.67e-34 132
MS.gene00274.t1 AT3G23560 26.171 363 246 2 18 377 37 380 6.19e-32 127
MS.gene00274.t1 AT4G25640 22.667 450 330 1 20 469 34 465 6.59e-32 128
MS.gene00274.t1 AT4G25640 22.667 450 330 1 20 469 34 465 8.12e-32 128
MS.gene00274.t1 AT5G10420 24.190 463 327 6 16 474 34 476 3.05e-31 126
MS.gene00274.t1 AT5G65380 24.846 487 333 5 2 477 14 478 5.30e-31 125
MS.gene00274.t1 AT1G33090 25.110 454 315 6 20 469 36 468 8.23e-31 125
MS.gene00274.t1 AT1G33100 24.558 452 320 4 20 469 33 465 6.72e-30 122
MS.gene00274.t1 AT1G47530 23.500 400 281 5 82 477 5 383 1.97e-29 120
MS.gene00274.t1 AT1G33080 23.611 432 301 4 49 474 65 473 1.85e-28 118
MS.gene00274.t1 AT1G33110 24.115 452 322 4 20 469 36 468 1.88e-28 118
MS.gene00274.t1 AT1G33080 23.653 427 297 4 49 469 65 468 7.83e-28 116
MS.gene00274.t1 AT2G34360 26.957 230 161 1 1 223 1 230 1.91e-27 115
MS.gene00274.t1 AT2G34360 35.366 164 104 1 312 475 257 418 1.43e-20 94.7
MS.gene00274.t1 AT4G25640 22.090 421 310 1 20 440 34 436 4.39e-27 114
MS.gene00274.t1 AT3G59030 26.099 364 251 6 34 396 65 411 1.49e-26 112
MS.gene00274.t1 AT3G03620 22.316 475 336 6 3 469 20 469 1.19e-24 107
MS.gene00274.t1 AT3G03620 22.316 475 336 6 3 469 20 469 1.19e-24 107
MS.gene00274.t1 AT5G17700 20.668 479 347 6 5 475 19 472 1.78e-21 97.8
MS.gene00274.t1 AT5G17700 20.668 479 347 6 5 475 19 472 1.78e-21 97.8

Find 124 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCTCACATTTGATTATT+TGG 0.125287 2.4:+55167125 None:intergenic
ACCGGTTACTCAGTCCTTTC+AGG 0.200835 2.4:-55168390 MS.gene00274:CDS
GTTCAACATTAGGGTTATTA+TGG 0.202311 2.4:-55167549 MS.gene00274:CDS
CAGTTGTCGGGTATGTCTTT+TGG 0.247868 2.4:+55168995 None:intergenic
GGAGTGCAAGGCCAGGTATT+GGG 0.276246 2.4:-55167384 MS.gene00274:CDS
AAGAATGGGGCATGTTAATA+AGG 0.284500 2.4:-55167831 MS.gene00274:CDS
GTTGACAAATTTGCCTTTGA+TGG 0.291782 2.4:+55167601 None:intergenic
TGATGAAGGGATAGCATAAA+TGG 0.299467 2.4:+55168225 None:intergenic
ACTATGCTCATGGCTGCTAT+TGG 0.316954 2.4:+55168510 None:intergenic
TCACTAGCAAGTTCAACATT+AGG 0.321641 2.4:-55167559 MS.gene00274:CDS
TGTAGAACATGTATGATAAA+AGG 0.326149 2.4:+55167950 None:intergenic
CACTAGCAAGTTCAACATTA+GGG 0.353538 2.4:-55167558 MS.gene00274:CDS
GAAGAAAAGAGTATTGAAAT+TGG 0.354879 2.4:+55167972 None:intergenic
AACTCACAAACACCAATTAT+AGG 0.359394 2.4:+55167448 None:intergenic
GGGTTATTATGGACAACATT+AGG 0.362622 2.4:-55167538 MS.gene00274:CDS
TGAGTTAGCAAGTGGTGCTT+TGG 0.364654 2.4:-55168433 MS.gene00274:CDS
AAAGGCAAATTTGTCAACTA+TGG 0.374974 2.4:-55167596 MS.gene00274:CDS
ACTAGTTGTGGAATGCTTAG+AGG 0.390010 2.4:-55167406 MS.gene00274:CDS
AGTGAAGTGCACCACAATAA+TGG 0.390331 2.4:+55168084 None:intergenic
GCAAGTGGTGCTTTGGCCAT+AGG 0.404906 2.4:-55168426 MS.gene00274:CDS
ATTAAGCCACAAGAGTGAAA+TGG 0.405706 2.4:+55168254 None:intergenic
CTATGCTCATGGCTGCTATT+GGG 0.407811 2.4:+55168511 None:intergenic
TTGTCAACTATGGTAGCAAT+TGG 0.413667 2.4:-55167586 MS.gene00274:CDS
GGGAGTGCAAGGCCAGGTAT+TGG 0.419436 2.4:-55167385 MS.gene00274:CDS
AAGGTTAAGAAAATGATGAT+AGG 0.422391 2.4:+55168042 None:intergenic
ACCTGAAAGGACTGAGTAAC+CGG 0.424046 2.4:+55168389 None:intergenic
AACCCCAACACTATTCCTAT+TGG 0.430761 2.4:+55167322 None:intergenic
GTCTTGGAGTTTGCTTAGAA+TGG 0.433878 2.4:-55167792 MS.gene00274:CDS
AGTTGTTTGCATGGGAAAGT+TGG 0.438028 2.4:-55168463 MS.gene00274:CDS
GCGCCAATAGGAATAGTGTT+GGG 0.442712 2.4:-55167325 MS.gene00274:CDS
CATGATCTTAGTTGTTTGCA+TGG 0.446886 2.4:-55168472 MS.gene00274:CDS
TTAAGCCACAAGAGTGAAAT+GGG 0.455813 2.4:+55168255 None:intergenic
GTTGTTTGCATGGGAAAGTT+GGG 0.457287 2.4:-55168462 MS.gene00274:CDS
ACCGACGGCACTTAGTGCTT+CGG 0.461028 2.4:-55167662 MS.gene00274:CDS
TACACCAAGGTGAAGTTTGA+AGG 0.461500 2.4:+55168023 None:intergenic
CATGCCCCATTCTTTGCCTA+GGG 0.463542 2.4:+55167840 None:intergenic
TTTATCCCTGCCCCAATACC+TGG 0.465679 2.4:+55167373 None:intergenic
ACATGCCCCATTCTTTGCCT+AGG 0.465944 2.4:+55167839 None:intergenic
CATTACCCATTTCACTCTTG+TGG 0.466604 2.4:-55168260 MS.gene00274:CDS
TTGTGGTATATAACACTGAT+TGG 0.471072 2.4:-55167222 MS.gene00274:CDS
TCAAAACCCTAGGCAAAGAA+TGG 0.471654 2.4:-55167846 MS.gene00274:CDS
ATAGCAGCCATGAGCATAGT+AGG 0.475953 2.4:-55168507 MS.gene00274:CDS
AACTCCAAGACAACTTTGAA+TGG 0.476284 2.4:+55167804 None:intergenic
CAAAACCCTAGGCAAAGAAT+GGG 0.476376 2.4:-55167845 MS.gene00274:CDS
GCAAGGCCAGGTATTGGGGC+AGG 0.476495 2.4:-55167379 MS.gene00274:CDS
GATGATAACAAGGGAGGACT+AGG 0.479205 2.4:+55167892 None:intergenic
ATAGGCTTCACCAATGTAAC+CGG 0.479266 2.4:-55168408 MS.gene00274:CDS
ATTCCACAACTAGTTGTTTG+TGG 0.480040 2.4:+55167415 None:intergenic
AAACTTCACCTTGGTGTACA+AGG 0.480431 2.4:-55168018 MS.gene00274:CDS
ATCGTACCTCAGCAGTTGTC+GGG 0.482070 2.4:+55168983 None:intergenic
CCCTTGTTGTGATGATAACA+AGG 0.485160 2.4:+55167882 None:intergenic
GAATCGAGAGGCGACAATAG+AGG 0.486395 2.4:+55168175 None:intergenic
GAGGTATCCTACTATGCTCA+TGG 0.490636 2.4:+55168500 None:intergenic
GAGTGCAAGGCCAGGTATTG+GGG 0.506676 2.4:-55167383 MS.gene00274:CDS
GGTTACATTGGTGAAGCCTA+TGG 0.510196 2.4:+55168410 None:intergenic
AACAAGGGAGGACTAGGAAT+AGG 0.516192 2.4:+55167898 None:intergenic
TACGTAGCAAAGGCACAACT+TGG 0.516312 2.4:-55168107 MS.gene00274:CDS
AATAGATGCGAATCGGATGA+AGG 0.516484 2.4:+55168130 None:intergenic
GCTTCGGTTTCAACAAGGGT+AGG 0.520507 2.4:-55167646 MS.gene00274:CDS
GGAAGAGCTAAAGAAAATGA+TGG 0.522010 2.4:-55168544 MS.gene00274:CDS
GGTGTTCACAAATGATAAAG+AGG 0.524145 2.4:-55167497 MS.gene00274:CDS
ATGATCTTAGTTGTTTGCAT+GGG 0.527005 2.4:-55168471 MS.gene00274:CDS
GAGGCTATTCGCTATAAGAT+CGG 0.528355 2.4:+55168152 None:intergenic
GAGTTTGCTTAGAATGGTGG+TGG 0.529737 2.4:-55167786 MS.gene00274:CDS
GTAGGGAATGCACTTGGAGC+TGG 0.530874 2.4:-55167628 MS.gene00274:CDS
CTACGTAAATAGATGCGAAT+CGG 0.532204 2.4:+55168123 None:intergenic
AGGCTATTCGCTATAAGATC+GGG 0.539074 2.4:+55168153 None:intergenic
CTAGTTGTGGAATGCTTAGA+GGG 0.539611 2.4:-55167405 MS.gene00274:CDS
CGTGTAGCTTTAGCCACGGC+GGG 0.541296 2.4:-55167721 MS.gene00274:CDS
TTCAGGTCTAGCCATGGGAA+TGG 0.547464 2.4:-55168373 MS.gene00274:CDS
CGCATCTATTTACGTAGCAA+AGG 0.547845 2.4:-55168117 MS.gene00274:CDS
GTCCTTTCAGGTCTAGCCAT+GGG 0.551373 2.4:-55168378 MS.gene00274:CDS
AGGTTAAGAAAATGATGATA+GGG 0.552617 2.4:+55168043 None:intergenic
AAGCTACACGAGGGTTGTAA+AGG 0.553073 2.4:+55167734 None:intergenic
TGTGGTATATAACACTGATT+GGG 0.556810 2.4:-55167221 MS.gene00274:CDS
GCACAACTTGGCCATTATTG+TGG 0.558363 2.4:-55168095 MS.gene00274:CDS
TGTGGTATCAATTCTTGTTG+TGG 0.558880 2.4:-55167239 MS.gene00274:CDS
TGGAGCTGGTCAACCATCAA+AGG 0.562155 2.4:-55167614 MS.gene00274:CDS
GACTGAGTAACCGGTTACAT+TGG 0.562773 2.4:+55168398 None:intergenic
GGGAGGACTAGGAATAGGTA+TGG 0.566306 2.4:+55167903 None:intergenic
GATAGGGATGTGAATGATAA+CGG 0.567233 2.4:+55168059 None:intergenic
CGCCAATAGGAATAGTGTTG+GGG 0.568084 2.4:-55167324 MS.gene00274:CDS
CTTAGAGGGAGTGCAAGGCC+AGG 0.570090 2.4:-55167391 MS.gene00274:CDS
GAGTTCATGACAATTCTAGC+TGG 0.573693 2.4:-55167760 MS.gene00274:CDS
CTAAAGAAAATGATGGACAT+AGG 0.574782 2.4:-55168537 MS.gene00274:CDS
CAAGGCCAGGTATTGGGGCA+GGG 0.575030 2.4:-55167378 MS.gene00274:CDS
TTGGAGTTTGCTTAGAATGG+TGG 0.575348 2.4:-55167789 MS.gene00274:CDS
CCTCTATGTACTCAAGCCAT+TGG 0.575821 2.4:-55168348 MS.gene00274:CDS
CCCTTGTTATCATCACAACA+AGG 0.576918 2.4:-55167883 MS.gene00274:CDS
CTTCGGTTTCAACAAGGGTA+GGG 0.577980 2.4:-55167645 MS.gene00274:CDS
TGTCCACAAACAACTAGTTG+TGG 0.580606 2.4:-55167418 MS.gene00274:CDS
GGCGCCAATAGGAATAGTGT+TGG 0.580799 2.4:-55167326 MS.gene00274:CDS
TATCGTACCTCAGCAGTTGT+CGG 0.582160 2.4:+55168982 None:intergenic
ACAAGGGTAGGGAATGCACT+TGG 0.586849 2.4:-55167634 MS.gene00274:CDS
TTAACCTTCAAACTTCACCT+TGG 0.590547 2.4:-55168027 MS.gene00274:CDS
TTGGGAAAGAGAGTCATTGA+AGG 0.590950 2.4:-55167203 MS.gene00274:CDS
GGTAGCCTTGAGTTAGCAAG+TGG 0.598563 2.4:-55168441 MS.gene00274:CDS
AGTCCTTTCAGGTCTAGCCA+TGG 0.601305 2.4:-55168379 MS.gene00274:CDS
CCCTCGTGTAGCTTTAGCCA+CGG 0.602755 2.4:-55167725 MS.gene00274:CDS
AAGCACCACTTGCTAACTCA+AGG 0.604905 2.4:+55168436 None:intergenic
GAATGCTTAGAGGGAGTGCA+AGG 0.609636 2.4:-55167396 MS.gene00274:CDS
TTAGTGCTTCGGTTTCAACA+AGG 0.611369 2.4:-55167651 MS.gene00274:CDS
CCAACAAGTGTCAAAACCCT+AGG 0.611672 2.4:-55167856 MS.gene00274:CDS
TTCCCATGGCTAGACCTGAA+AGG 0.612296 2.4:+55168376 None:intergenic
ACATAGAGGCTCCATTCCCA+TGG 0.614544 2.4:+55168362 None:intergenic
CCAATGGCTTGAGTACATAG+AGG 0.624591 2.4:+55168348 None:intergenic
ACTTATGTACACTTTACCGA+CGG 0.625353 2.4:-55167677 MS.gene00274:CDS
TTGTATTACTATTCCCGCCG+TGG 0.632361 2.4:+55167708 None:intergenic
TAGTGCTTCGGTTTCAACAA+GGG 0.633465 2.4:-55167650 MS.gene00274:CDS
ACCGAAGCACTAAGTGCCGT+CGG 0.645077 2.4:+55167661 None:intergenic
CCTTGTTGTGATGATAACAA+GGG 0.647360 2.4:+55167883 None:intergenic
GGTGTTAGAGTTGACAATGG+CGG 0.648706 2.4:-55167476 MS.gene00274:CDS
GACATACCCGACAACTGCTG+AGG 0.660676 2.4:-55168989 MS.gene00274:intron
CTATAAGATCGGGAATCGAG+AGG 0.665968 2.4:+55168163 None:intergenic
GGCAGCACAAATAGCATGTG+TGG 0.671756 2.4:-55167257 MS.gene00274:CDS
GCCGTGGCTAAAGCTACACG+AGG 0.684997 2.4:+55167724 None:intergenic
TGTTGTGATGATAACAAGGG+AGG 0.686225 2.4:+55167886 None:intergenic
AGATGCGAATCGGATGAAGG+AGG 0.692679 2.4:+55168133 None:intergenic
CCTTGTTATCATCACAACAA+GGG 0.693087 2.4:-55167882 MS.gene00274:CDS
AAAACCCTAGGCAAAGAATG+GGG 0.698680 2.4:-55167844 MS.gene00274:CDS
AGAGGTGTTAGAGTTGACAA+TGG 0.702435 2.4:-55167479 MS.gene00274:CDS
TCGTGTAGCTTTAGCCACGG+CGG 0.710232 2.4:-55167722 MS.gene00274:CDS
TAGCAATACCTTGTACACCA+AGG 0.710910 2.4:+55168010 None:intergenic
CCGTGGCTAAAGCTACACGA+GGG 0.768897 2.4:+55167725 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTACTCATTTTACT+TGG - chr2.4:55168758-55168777 MS.gene00274:intron 15.0%
!! TTATGATATTGATGAAGAAA+TGG + chr2.4:55167374-55167393 None:intergenic 20.0%
!!! TATATCTTGATTTTGATGAA+GGG + chr2.4:55167902-55167921 None:intergenic 20.0%
!!! TGTTTTTGTTTTGTTTTTTC+AGG - chr2.4:55167540-55167559 MS.gene00274:CDS 20.0%
!!! TTATATCTTGATTTTGATGA+AGG + chr2.4:55167903-55167922 None:intergenic 20.0%
! AACATTAGGAAGAGAAAAAT+GGG - chr2.4:55168587-55168606 MS.gene00274:intron 25.0%
! AAGGTTAAGAAAATGATGAT+AGG + chr2.4:55168072-55168091 None:intergenic 25.0%
! ACTATGAGTATATAGTATGA+GGG + chr2.4:55167212-55167231 None:intergenic 25.0%
! ACTCATAGTATTTACTTACA+TGG - chr2.4:55167223-55167242 MS.gene00274:CDS 25.0%
! AGGTTAAGAAAATGATGATA+GGG + chr2.4:55168071-55168090 None:intergenic 25.0%
! GAAATGGTATATGAAAACTA+TGG + chr2.4:55167358-55167377 None:intergenic 25.0%
! GCATAGCATATATATTAACA+TGG - chr2.4:55167428-55167447 MS.gene00274:CDS 25.0%
! TACTATGAGTATATAGTATG+AGG + chr2.4:55167213-55167232 None:intergenic 25.0%
! TGTAGAACATGTATGATAAA+AGG + chr2.4:55168164-55168183 None:intergenic 25.0%
!! TTTTACTCATTTTACTTGGT+GGG - chr2.4:55168762-55168781 MS.gene00274:intron 25.0%
!! TTTTTACTCATTTTACTTGG+TGG - chr2.4:55168761-55168780 MS.gene00274:intron 25.0%
!!! GAAGAAAAGAGTATTGAAAT+TGG + chr2.4:55168142-55168161 None:intergenic 25.0%
!!! TTTTGTTTTGTTTTTTCAGG+TGG - chr2.4:55167543-55167562 MS.gene00274:CDS 25.0%
AAAGGCAAATTTGTCAACTA+TGG - chr2.4:55168515-55168534 MS.gene00274:CDS 30.0%
AACTCACAAACACCAATTAT+AGG + chr2.4:55168666-55168685 None:intergenic 30.0%
ACATTAGGAAGAGAAAAATG+GGG - chr2.4:55168588-55168607 MS.gene00274:intron 30.0%
ATACCATACAAAATCTCTCA+TGG - chr2.4:55167153-55167172 MS.gene00274:CDS 30.0%
ATGATCTTAGTTGTTTGCAT+GGG - chr2.4:55167640-55167659 MS.gene00274:CDS 30.0%
CAACATTAGGAAGAGAAAAA+TGG - chr2.4:55168586-55168605 MS.gene00274:intron 30.0%
CTTGCTCACATTTGATTATT+TGG + chr2.4:55168989-55169008 None:intergenic 30.0%
GTTCAACATTAGGGTTATTA+TGG - chr2.4:55168562-55168581 MS.gene00274:intron 30.0%
TTGTGGTATATAACACTGAT+TGG - chr2.4:55168889-55168908 MS.gene00274:intron 30.0%
! AAACCATGAGAGATTTTGTA+TGG + chr2.4:55167159-55167178 None:intergenic 30.0%
! TGTGGTATATAACACTGATT+GGG - chr2.4:55168890-55168909 MS.gene00274:intron 30.0%
! TTTTCCATTCAAAGTTGTCT+TGG - chr2.4:55168303-55168322 MS.gene00274:CDS 30.0%
!! CAACTAAGATCATGTTTTTG+AGG + chr2.4:55167633-55167652 None:intergenic 30.0%
!! CTAAAGAAAATGATGGACAT+AGG - chr2.4:55167574-55167593 MS.gene00274:CDS 30.0%
!! TTTACTCATTTTACTTGGTG+GGG - chr2.4:55168763-55168782 MS.gene00274:intron 30.0%
!!! GTTTGTTTTGAAATTAGGGT+TGG - chr2.4:55168809-55168828 MS.gene00274:intron 30.0%
!!! TGGGGTTTGTTTTGAAATTA+GGG - chr2.4:55168805-55168824 MS.gene00274:intron 30.0%
!!! TTGGGGTTTGTTTTGAAATT+AGG - chr2.4:55168804-55168823 MS.gene00274:intron 30.0%
AACTCCAAGACAACTTTGAA+TGG + chr2.4:55168310-55168329 None:intergenic 35.0%
AAGAATGGGGCATGTTAATA+AGG - chr2.4:55168280-55168299 MS.gene00274:CDS 35.0%
ACTCTGTTATTGGAGAAAGA+TGG - chr2.4:55167468-55167487 MS.gene00274:CDS 35.0%
ACTTATGTACACTTTACCGA+CGG - chr2.4:55168434-55168453 MS.gene00274:CDS 35.0%
ATTAAGCCACAAGAGTGAAA+TGG + chr2.4:55167860-55167879 None:intergenic 35.0%
ATTCCACAACTAGTTGTTTG+TGG + chr2.4:55168699-55168718 None:intergenic 35.0%
CACTAGCAAGTTCAACATTA+GGG - chr2.4:55168553-55168572 MS.gene00274:intron 35.0%
CATGATCTTAGTTGTTTGCA+TGG - chr2.4:55167639-55167658 MS.gene00274:CDS 35.0%
CATTAGGAAGAGAAAAATGG+GGG - chr2.4:55168589-55168608 MS.gene00274:intron 35.0%
CTACGTAAATAGATGCGAAT+CGG + chr2.4:55167991-55168010 None:intergenic 35.0%
CTCTGTTATTGGAGAAAGAT+GGG - chr2.4:55167469-55167488 MS.gene00274:CDS 35.0%
GATAGGGATGTGAATGATAA+CGG + chr2.4:55168055-55168074 None:intergenic 35.0%
GGAAGAGCTAAAGAAAATGA+TGG - chr2.4:55167567-55167586 MS.gene00274:CDS 35.0%
GGGTTATTATGGACAACATT+AGG - chr2.4:55168573-55168592 MS.gene00274:intron 35.0%
GGTGTTCACAAATGATAAAG+AGG - chr2.4:55168614-55168633 MS.gene00274:intron 35.0%
GTTGACAAATTTGCCTTTGA+TGG + chr2.4:55168513-55168532 None:intergenic 35.0%
TCACTAGCAAGTTCAACATT+AGG - chr2.4:55168552-55168571 MS.gene00274:CDS 35.0%
TCTGTTATTGGAGAAAGATG+GGG - chr2.4:55167470-55167489 MS.gene00274:CDS 35.0%
TGATGAAGGGATAGCATAAA+TGG + chr2.4:55167889-55167908 None:intergenic 35.0%
TGTGGTATCAATTCTTGTTG+TGG - chr2.4:55168872-55168891 MS.gene00274:intron 35.0%
TGTTATAGCCGACCTTTAAT+CGG - chr2.4:55167309-55167328 MS.gene00274:CDS 35.0%
TTAACCTTCAAACTTCACCT+TGG - chr2.4:55168084-55168103 MS.gene00274:CDS 35.0%
TTAAGCCACAAGAGTGAAAT+GGG + chr2.4:55167859-55167878 None:intergenic 35.0%
TTGTCAACTATGGTAGCAAT+TGG - chr2.4:55168525-55168544 MS.gene00274:CDS 35.0%
! ATGGCGGTTTTACCTATAAT+TGG - chr2.4:55168651-55168670 MS.gene00274:intron 35.0%
! CCTTGTTATCATCACAACAA+GGG - chr2.4:55168229-55168248 MS.gene00274:CDS 35.0%
! CCTTGTTGTGATGATAACAA+GGG + chr2.4:55168231-55168250 None:intergenic 35.0%
!! AGAGAAGTTTTGTGAACCAA+TGG + chr2.4:55167782-55167801 None:intergenic 35.0%
!! TTACTCATTTTACTTGGTGG+GGG - chr2.4:55168764-55168783 MS.gene00274:intron 35.0%
!!! GTTTTGAAATTAGGGTTGGT+GGG - chr2.4:55168813-55168832 MS.gene00274:intron 35.0%
!!! TGTTTTGAAATTAGGGTTGG+TGG - chr2.4:55168812-55168831 MS.gene00274:intron 35.0%
!!! TGTTTTGTTTTTTCAGGTGG+TGG - chr2.4:55167546-55167565 MS.gene00274:CDS 35.0%
!!! TTTTGAAATTAGGGTTGGTG+GGG - chr2.4:55168814-55168833 MS.gene00274:intron 35.0%
AAAACCCTAGGCAAAGAATG+GGG - chr2.4:55168267-55168286 MS.gene00274:CDS 40.0%
AACCCCAACACTATTCCTAT+TGG + chr2.4:55168792-55168811 None:intergenic 40.0%
AATAGATGCGAATCGGATGA+AGG + chr2.4:55167984-55168003 None:intergenic 40.0%
ACTAGTTGTGGAATGCTTAG+AGG - chr2.4:55168705-55168724 MS.gene00274:intron 40.0%
AGGCTATTCGCTATAAGATC+GGG + chr2.4:55167961-55167980 None:intergenic 40.0%
AGTGAAGTGCACCACAATAA+TGG + chr2.4:55168030-55168049 None:intergenic 40.0%
AGTTGTTTGCATGGGAAAGT+TGG - chr2.4:55167648-55167667 MS.gene00274:CDS 40.0%
ATAGGCTTCACCAATGTAAC+CGG - chr2.4:55167703-55167722 MS.gene00274:CDS 40.0%
ATTGAAGGCAAAAAGCTTGG+TGG - chr2.4:55168923-55168942 MS.gene00274:intron 40.0%
CAAAACCCTAGGCAAAGAAT+GGG - chr2.4:55168266-55168285 MS.gene00274:CDS 40.0%
CATTACCCATTTCACTCTTG+TGG - chr2.4:55167851-55167870 MS.gene00274:CDS 40.0%
CGCATCTATTTACGTAGCAA+AGG - chr2.4:55167994-55168013 MS.gene00274:CDS 40.0%
CTAGTTGTGGAATGCTTAGA+GGG - chr2.4:55168706-55168725 MS.gene00274:intron 40.0%
CTTTCTCCAATAACAGAGTG+AGG + chr2.4:55167467-55167486 None:intergenic 40.0%
GAGGCTATTCGCTATAAGAT+CGG + chr2.4:55167962-55167981 None:intergenic 40.0%
GAGTTCATGACAATTCTAGC+TGG - chr2.4:55168351-55168370 MS.gene00274:CDS 40.0%
GTCATTGAAGGCAAAAAGCT+TGG - chr2.4:55168920-55168939 MS.gene00274:intron 40.0%
GTTGTTTGCATGGGAAAGTT+GGG - chr2.4:55167649-55167668 MS.gene00274:CDS 40.0%
TACACCAAGGTGAAGTTTGA+AGG + chr2.4:55168091-55168110 None:intergenic 40.0%
TAGCAATACCTTGTACACCA+AGG + chr2.4:55168104-55168123 None:intergenic 40.0%
TAGTGCTTCGGTTTCAACAA+GGG - chr2.4:55168461-55168480 MS.gene00274:CDS 40.0%
TCAAAACCCTAGGCAAAGAA+TGG - chr2.4:55168265-55168284 MS.gene00274:CDS 40.0%
TGTCCACAAACAACTAGTTG+TGG - chr2.4:55168693-55168712 MS.gene00274:intron 40.0%
TGTTGTGATGATAACAAGGG+AGG + chr2.4:55168228-55168247 None:intergenic 40.0%
TTAGTGCTTCGGTTTCAACA+AGG - chr2.4:55168460-55168479 MS.gene00274:CDS 40.0%
TTGAAGGCAAAAAGCTTGGT+GGG - chr2.4:55168924-55168943 MS.gene00274:intron 40.0%
TTGGGAAAGAGAGTCATTGA+AGG - chr2.4:55168908-55168927 MS.gene00274:intron 40.0%
! AAACTTCACCTTGGTGTACA+AGG - chr2.4:55168093-55168112 MS.gene00274:CDS 40.0%
! AGTTGTCGGGTATGTCTTTT+GGG + chr2.4:55167118-55167137 None:intergenic 40.0%
! CCCTTGTTATCATCACAACA+AGG - chr2.4:55168228-55168247 MS.gene00274:CDS 40.0%
! CCCTTGTTGTGATGATAACA+AGG + chr2.4:55168232-55168251 None:intergenic 40.0%
! GTCTTGGAGTTTGCTTAGAA+TGG - chr2.4:55168319-55168338 MS.gene00274:CDS 40.0%
! TTGGAGTTTGCTTAGAATGG+TGG - chr2.4:55168322-55168341 MS.gene00274:CDS 40.0%
!! AGAGGTGTTAGAGTTGACAA+TGG - chr2.4:55168632-55168651 MS.gene00274:intron 40.0%
!! AGCAGTTGATGACATTTGAC+CGG + chr2.4:55167271-55167290 None:intergenic 40.0%
!!! GGGGTTTTGTTATGGTTTGT+TGG - chr2.4:55168833-55168852 MS.gene00274:intron 40.0%
AACAAGGGAGGACTAGGAAT+AGG + chr2.4:55168216-55168235 None:intergenic 45.0%
AACAGAGTGAGGCTGCATAA+AGG + chr2.4:55167456-55167475 None:intergenic 45.0%
AAGCTACACGAGGGTTGTAA+AGG + chr2.4:55168380-55168399 None:intergenic 45.0%
ACCTGAAAGGACTGAGTAAC+CGG + chr2.4:55167725-55167744 None:intergenic 45.0%
ACTATGCTCATGGCTGCTAT+TGG + chr2.4:55167604-55167623 None:intergenic 45.0%
AGGAAGAGAAAAATGGGGGA+AGG - chr2.4:55168593-55168612 MS.gene00274:intron 45.0%
ATAGCAGCCATGAGCATAGT+AGG - chr2.4:55167604-55167623 MS.gene00274:CDS 45.0%
ATAGCCGACCTTTAATCGGA+GGG - chr2.4:55167313-55167332 MS.gene00274:CDS 45.0%
ATGCAGCCTCACTCTGTTAT+TGG - chr2.4:55167458-55167477 MS.gene00274:CDS 45.0%
CCAACAAGTGTCAAAACCCT+AGG - chr2.4:55168255-55168274 MS.gene00274:CDS 45.0%
CCAATGGCTTGAGTACATAG+AGG + chr2.4:55167766-55167785 None:intergenic 45.0%
CCTCTATGTACTCAAGCCAT+TGG - chr2.4:55167763-55167782 MS.gene00274:CDS 45.0%
CGCCAATAGGAATAGTGTTG+GGG - chr2.4:55168787-55168806 MS.gene00274:intron 45.0%
CTATAAGATCGGGAATCGAG+AGG + chr2.4:55167951-55167970 None:intergenic 45.0%
CTATGCTCATGGCTGCTATT+GGG + chr2.4:55167603-55167622 None:intergenic 45.0%
CTTCGGTTTCAACAAGGGTA+GGG - chr2.4:55168466-55168485 MS.gene00274:CDS 45.0%
GACTGAGTAACCGGTTACAT+TGG + chr2.4:55167716-55167735 None:intergenic 45.0%
GATGATAACAAGGGAGGACT+AGG + chr2.4:55168222-55168241 None:intergenic 45.0%
GCACAACTTGGCCATTATTG+TGG - chr2.4:55168016-55168035 MS.gene00274:CDS 45.0%
GCGCCAATAGGAATAGTGTT+GGG - chr2.4:55168786-55168805 MS.gene00274:intron 45.0%
GGTGTTAGAGTTGACAATGG+CGG - chr2.4:55168635-55168654 MS.gene00274:intron 45.0%
GGTTACATTGGTGAAGCCTA+TGG + chr2.4:55167704-55167723 None:intergenic 45.0%
GTTATGCTCCCTCCGATTAA+AGG + chr2.4:55167324-55167343 None:intergenic 45.0%
TATAGCCGACCTTTAATCGG+AGG - chr2.4:55167312-55167331 MS.gene00274:CDS 45.0%
TATCGTACCTCAGCAGTTGT+CGG + chr2.4:55167132-55167151 None:intergenic 45.0%
TGAGTTAGCAAGTGGTGCTT+TGG - chr2.4:55167678-55167697 MS.gene00274:CDS 45.0%
TTGTATTACTATTCCCGCCG+TGG + chr2.4:55168406-55168425 None:intergenic 45.0%
! AAGCACCACTTGCTAACTCA+AGG + chr2.4:55167678-55167697 None:intergenic 45.0%
! CAGTTGTCGGGTATGTCTTT+TGG + chr2.4:55167119-55167138 None:intergenic 45.0%
! GAGGTATCCTACTATGCTCA+TGG + chr2.4:55167614-55167633 None:intergenic 45.0%
! GAGTTTGCTTAGAATGGTGG+TGG - chr2.4:55168325-55168344 MS.gene00274:CDS 45.0%
! TACGTAGCAAAGGCACAACT+TGG - chr2.4:55168004-55168023 MS.gene00274:CDS 45.0%
!! CCTAGGGTTTTGACACTTGT+TGG + chr2.4:55168258-55168277 None:intergenic 45.0%
ACAAGGGTAGGGAATGCACT+TGG - chr2.4:55168477-55168496 MS.gene00274:CDS 50.0%
ACATAGAGGCTCCATTCCCA+TGG + chr2.4:55167752-55167771 None:intergenic 50.0%
ACATGCCCCATTCTTTGCCT+AGG + chr2.4:55168275-55168294 None:intergenic 50.0%
ACCGGTTACTCAGTCCTTTC+AGG - chr2.4:55167721-55167740 MS.gene00274:CDS 50.0%
AGATGCGAATCGGATGAAGG+AGG + chr2.4:55167981-55168000 None:intergenic 50.0%
ATCGTACCTCAGCAGTTGTC+GGG + chr2.4:55167131-55167150 None:intergenic 50.0%
CATGCCCCATTCTTTGCCTA+GGG + chr2.4:55168274-55168293 None:intergenic 50.0%
GAATCGAGAGGCGACAATAG+AGG + chr2.4:55167939-55167958 None:intergenic 50.0%
GCTTCGGTTTCAACAAGGGT+AGG - chr2.4:55168465-55168484 MS.gene00274:CDS 50.0%
GGCAGCACAAATAGCATGTG+TGG - chr2.4:55168854-55168873 MS.gene00274:intron 50.0%
GGCGCCAATAGGAATAGTGT+TGG - chr2.4:55168785-55168804 MS.gene00274:intron 50.0%
GGGAGGACTAGGAATAGGTA+TGG + chr2.4:55168211-55168230 None:intergenic 50.0%
TGACATTTGACCGGAGAGAG+AGG + chr2.4:55167262-55167281 None:intergenic 50.0%
TGCTCCCTCCGATTAAAGGT+CGG + chr2.4:55167320-55167339 None:intergenic 50.0%
TGGAGCTGGTCAACCATCAA+AGG - chr2.4:55168497-55168516 MS.gene00274:CDS 50.0%
TTCAGGTCTAGCCATGGGAA+TGG - chr2.4:55167738-55167757 MS.gene00274:CDS 50.0%
TTCCCATGGCTAGACCTGAA+AGG + chr2.4:55167738-55167757 None:intergenic 50.0%
TTTATCCCTGCCCCAATACC+TGG + chr2.4:55168741-55168760 None:intergenic 50.0%
! AGTCCTTTCAGGTCTAGCCA+TGG - chr2.4:55167732-55167751 MS.gene00274:CDS 50.0%
! GAATGCTTAGAGGGAGTGCA+AGG - chr2.4:55168715-55168734 MS.gene00274:intron 50.0%
! GGTAGCCTTGAGTTAGCAAG+TGG - chr2.4:55167670-55167689 MS.gene00274:CDS 50.0%
! GTCCTTTCAGGTCTAGCCAT+GGG - chr2.4:55167733-55167752 MS.gene00274:CDS 50.0%
!!! GGGTTGGTGGGGTTTTGTTA+TGG - chr2.4:55168825-55168844 MS.gene00274:intron 50.0%
ACCGACGGCACTTAGTGCTT+CGG - chr2.4:55168449-55168468 MS.gene00274:CDS 55.0%
CATTTGACCGGAGAGAGAGG+CGG + chr2.4:55167259-55167278 None:intergenic 55.0%
CCGTGGCTAAAGCTACACGA+GGG + chr2.4:55168389-55168408 None:intergenic 55.0%
GACATACCCGACAACTGCTG+AGG - chr2.4:55167122-55167141 MS.gene00274:CDS 55.0%
GCAAGTGGTGCTTTGGCCAT+AGG - chr2.4:55167685-55167704 MS.gene00274:CDS 55.0%
GGAGTGCAAGGCCAGGTATT+GGG - chr2.4:55168727-55168746 MS.gene00274:intron 55.0%
GGTCGGCTATAACAGCTCTC+TGG + chr2.4:55167303-55167322 None:intergenic 55.0%
GTAGGGAATGCACTTGGAGC+TGG - chr2.4:55168483-55168502 MS.gene00274:CDS 55.0%
! ACCGAAGCACTAAGTGCCGT+CGG + chr2.4:55168453-55168472 None:intergenic 55.0%
! CTTTTTGCCGCCTCTCTCTC+CGG - chr2.4:55167249-55167268 MS.gene00274:CDS 55.0%
! GAGTGCAAGGCCAGGTATTG+GGG - chr2.4:55168728-55168747 MS.gene00274:intron 55.0%
!! CCCTCGTGTAGCTTTAGCCA+CGG - chr2.4:55168386-55168405 MS.gene00274:CDS 55.0%
!! TCGTGTAGCTTTAGCCACGG+CGG - chr2.4:55168389-55168408 MS.gene00274:CDS 55.0%
CGGAGAGAGAGGCGGCAAAA+AGG + chr2.4:55167251-55167270 None:intergenic 60.0%
GCCGTGGCTAAAGCTACACG+AGG + chr2.4:55168390-55168409 None:intergenic 60.0%
! CTTAGAGGGAGTGCAAGGCC+AGG - chr2.4:55168720-55168739 MS.gene00274:intron 60.0%
! GGGAGTGCAAGGCCAGGTAT+TGG - chr2.4:55168726-55168745 MS.gene00274:intron 60.0%
!! CAAGGCCAGGTATTGGGGCA+GGG - chr2.4:55168733-55168752 MS.gene00274:intron 60.0%
!! CGTGTAGCTTTAGCCACGGC+GGG - chr2.4:55168390-55168409 MS.gene00274:CDS 60.0%
!! TACTTGGTGGGGGCGCCAAT+AGG - chr2.4:55168774-55168793 MS.gene00274:intron 60.0%
!! GCAAGGCCAGGTATTGGGGC+AGG - chr2.4:55168732-55168751 MS.gene00274:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 55167096 55169037 55167096 ID=MS.gene00274
chr2.4 mRNA 55167096 55169037 55167096 ID=MS.gene00274.t1;Parent=MS.gene00274
chr2.4 exon 55168990 55169037 55168990 ID=MS.gene00274.t1.exon1;Parent=MS.gene00274.t1
chr2.4 CDS 55168990 55169037 55168990 ID=cds.MS.gene00274.t1;Parent=MS.gene00274.t1
chr2.4 exon 55167096 55168571 55167096 ID=MS.gene00274.t1.exon2;Parent=MS.gene00274.t1
chr2.4 CDS 55167096 55168571 55167096 ID=cds.MS.gene00274.t1;Parent=MS.gene00274.t1
Gene Sequence

>MS.gene00274

ATGGTTGCTCAAGAGAAATCCCAAAAGACATACCCGACAACTGCTGAGGTACGATATATACCATACAAAATCTCTCATGGTTTTCACATTTTTCATACATATCACTTCTTCATCCCTCATACTATATACTCATAGTATTTACTTACATGGATCCTTTTTGCCGCCTCTCTCTCCGGTCAAATGTCATCAACTGCTGACATTTGACCAGAGAGCTGTTATAGCCGACCTTTAATCGGAGGGAGCATAACATATTAAAATTCCATAGTTTTCATATACCATTTCTTCATCAATATCATAACATAGCATTAGTTCAACAAAAAAAAATTATCATAGCATAGCATATATATTAACATGGATCCTTTATGCAGCCTCACTCTGTTATTGGAGAAAGATGGGGCTGTTGAGAAGCTATGTCTTATAGTATATACTTGTAAAAGTTCATATTGTTTTTGTTTTGTTTTTTCAGGTGGTGGAAGAGCTAAAGAAAATGATGGACATAGGTTTCCCAATAGCAGCCATGAGCATAGTAGGATACCTCAAAAACATGATCTTAGTTGTTTGCATGGGAAAGTTGGGTAGCCTTGAGTTAGCAAGTGGTGCTTTGGCCATAGGCTTCACCAATGTAACCGGTTACTCAGTCCTTTCAGGTCTAGCCATGGGAATGGAGCCTCTATGTACTCAAGCCATTGGTTCACAAAACTTCTCTTTAGTCTCTCTCATATTAAGAAGAACAATTCTCATGTTATTAGTAGCTTCATTACCCATTTCACTCTTGTGGCTTAATCTTGAACCATTTATGCTATCCCTTCATCAAAATCAAGATATAACAAGAATAGCAAGCCTCTATTGTCGCCTCTCGATTCCCGATCTTATAGCGAATAGCCTCCTTCATCCGATTCGCATCTATTTACGTAGCAAAGGCACAACTTGGCCATTATTGTGGTGCACTTCACTTTCCGTTATCATTCACATCCCTATCATCATTTTCTTAACCTTCAAACTTCACCTTGGTGTACAAGGTATTGCTATTTCAGCTTTTGTTGCCAATTTCAATACTCTTTTCTTCCTTTTATCATACATGTTCTACATGCATGTCTCACATGTTTCAATCTCCATACCTATTCCTAGTCCTCCCTTGTTATCATCACAACAAGGGAAACCAACAAGTGTCAAAACCCTAGGCAAAGAATGGGGCATGTTAATAAGGTTTTCCATTCAAAGTTGTCTTGGAGTTTGCTTAGAATGGTGGTGGTATGAGTTCATGACAATTCTAGCTGGTTACCTTTACAACCCTCGTGTAGCTTTAGCCACGGCGGGAATAGTAATACAAACAACATCACTTATGTACACTTTACCGACGGCACTTAGTGCTTCGGTTTCAACAAGGGTAGGGAATGCACTTGGAGCTGGTCAACCATCAAAGGCAAATTTGTCAACTATGGTAGCAATTGGAATGTCACTAGCAAGTTCAACATTAGGGTTATTATGGACAACATTAGGAAGAGAAAAATGGGGGAAGGTGTTCACAAATGATAAAGAGGTGTTAGAGTTGACAATGGCGGTTTTACCTATAATTGGTGTTTGTGAGTTAGCAAATTGTCCACAAACAACTAGTTGTGGAATGCTTAGAGGGAGTGCAAGGCCAGGTATTGGGGCAGGGATAAATTTTTACTCATTTTACTTGGTGGGGGCGCCAATAGGAATAGTGTTGGGGTTTGTTTTGAAATTAGGGTTGGTGGGGTTTTGTTATGGTTTGTTGGCAGCACAAATAGCATGTGTGGTATCAATTCTTGTTGTGGTATATAACACTGATTGGGAAAGAGAGTCATTGAAGGCAAAAAGCTTGGTGGGTAATGATACATGTGACACATTATTTGCTCATGTTGAAGACCAAATAATCAAATGTGAGCAAGATATTGTCTTTCTCAATGAGAACAAGTGA
Protein sequence

>MS.gene00274.t1

MVAQEKSQKTYPTTAEVVEELKKMMDIGFPIAAMSIVGYLKNMILVVCMGKLGSLELASGALAIGFTNVTGYSVLSGLAMGMEPLCTQAIGSQNFSLVSLILRRTILMLLVASLPISLLWLNLEPFMLSLHQNQDITRIASLYCRLSIPDLIANSLLHPIRIYLRSKGTTWPLLWCTSLSVIIHIPIIIFLTFKLHLGVQGIAISAFVANFNTLFFLLSYMFYMHVSHVSISIPIPSPPLLSSQQGKPTSVKTLGKEWGMLIRFSIQSCLGVCLEWWWYEFMTILAGYLYNPRVALATAGIVIQTTSLMYTLPTALSASVSTRVGNALGAGQPSKANLSTMVAIGMSLASSTLGLLWTTLGREKWGKVFTNDKEVLELTMAVLPIIGVCELANCPQTTSCGMLRGSARPGIGAGINFYSFYLVGAPIGIVLGFVLKLGLVGFCYGLLAAQIACVVSILVVVYNTDWERESLKAKSLVGNDTCDTLFAHVEDQIIKCEQDIVFLNENK