AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072817

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072817.t1 MTR_5g010830 98.834 429 5 0 1 429 92 520 0.0 861
MS.gene072817.t1 MTR_1g101500 73.804 439 105 2 1 429 91 529 0.0 656
MS.gene072817.t1 MTR_0039s0080 71.462 431 121 1 1 429 65 495 0.0 625
MS.gene072817.t1 MTR_5g067460 63.318 428 157 0 1 428 82 509 0.0 573
MS.gene072817.t1 MTR_3g111020 62.708 421 157 0 1 421 78 498 0.0 556
MS.gene072817.t1 MTR_6g088805 55.245 429 189 1 1 429 54 479 7.00e-173 493
MS.gene072817.t1 MTR_8g102430 54.567 416 169 2 1 416 50 445 1.74e-162 465
MS.gene072817.t1 MTR_4g027920 52.204 431 204 1 1 429 183 613 4.24e-155 452
MS.gene072817.t1 MTR_6g043810 50.226 442 202 5 2 428 61 499 2.07e-152 442
MS.gene072817.t1 MTR_6g043810 50.113 443 203 5 1 428 50 489 2.28e-152 441
MS.gene072817.t1 MTR_2g435930 45.517 435 221 2 4 422 49 483 4.77e-128 379
MS.gene072817.t1 MTR_6g081410 34.824 425 275 1 1 423 52 476 2.72e-88 276
MS.gene072817.t1 MTR_5g011540 38.588 425 254 3 3 423 72 493 6.26e-86 271
MS.gene072817.t1 MTR_6g081810 34.507 426 247 3 1 423 54 450 4.65e-85 267
MS.gene072817.t1 MTR_6g066380 34.965 429 271 3 1 424 54 479 3.56e-84 266
MS.gene072817.t1 MTR_6g081300 35.680 412 265 0 1 412 37 448 4.55e-84 265
MS.gene072817.t1 MTR_8g106670 36.493 422 252 7 1 414 39 452 4.75e-84 266
MS.gene072817.t1 MTR_6g081780 33.894 416 273 1 1 414 52 467 3.23e-83 263
MS.gene072817.t1 MTR_6g081400 33.333 429 277 3 1 424 42 466 1.37e-81 259
MS.gene072817.t1 MTR_6g081310 35.211 426 260 4 1 412 23 446 1.11e-79 254
MS.gene072817.t1 MTR_1g108840 34.053 417 269 2 1 414 68 481 1.35e-79 254
MS.gene072817.t1 MTR_6g081240 34.474 409 267 1 1 408 36 444 3.82e-79 252
MS.gene072817.t1 MTR_1g108810 33.894 416 263 4 1 410 61 470 1.61e-76 246
MS.gene072817.t1 MTR_6g081390 31.566 415 283 1 1 414 39 453 1.38e-71 233
MS.gene072817.t1 MTR_6g004220 32.938 422 268 6 1 414 50 464 1.01e-70 231
MS.gene072817.t1 MTR_2g078930 33.724 427 271 4 1 424 70 487 1.40e-69 228
MS.gene072817.t1 MTR_2g088400 29.717 424 297 1 2 424 80 503 4.64e-69 227
MS.gene072817.t1 MTR_6g004220 32.692 416 265 6 1 408 57 465 1.16e-67 223
MS.gene072817.t1 MTR_7g082810 30.892 437 288 5 1 428 47 478 2.21e-67 222
MS.gene072817.t1 MTR_1g109060 33.413 419 269 4 1 414 51 464 9.88e-65 215
MS.gene072817.t1 MTR_2g079020 33.645 428 270 6 1 424 64 481 1.34e-64 216
MS.gene072817.t1 MTR_4g048000 28.066 424 304 1 2 424 92 515 1.85e-64 216
MS.gene072817.t1 MTR_2g078960 33.898 413 250 5 1 402 60 460 4.44e-62 208
MS.gene072817.t1 MTR_1g109060 33.172 413 266 4 1 408 51 458 4.84e-62 208
MS.gene072817.t1 MTR_2g078970 32.710 428 274 6 1 424 58 475 1.17e-61 207
MS.gene072817.t1 MTR_1g108990 31.604 424 282 4 1 420 52 471 2.33e-61 206
MS.gene072817.t1 MTR_7g082800 31.750 400 259 5 33 425 1 393 8.59e-61 202
MS.gene072817.t1 MTR_2g022980 29.126 412 292 0 3 414 77 488 2.85e-60 204
MS.gene072817.t1 MTR_2g088400 30.159 378 264 0 2 379 80 457 1.75e-59 201
MS.gene072817.t1 MTR_8g069470 29.252 441 289 7 2 426 78 511 4.32e-59 201
MS.gene072817.t1 MTR_8g063590 31.628 430 283 7 1 424 80 504 4.75e-57 196
MS.gene072817.t1 MTR_1g021740 29.426 418 282 5 3 414 64 474 3.03e-56 193
MS.gene072817.t1 MTR_5g032720 29.577 426 296 3 3 426 65 488 6.65e-55 189
MS.gene072817.t1 MTR_6g047610 27.934 426 304 2 2 427 59 481 1.68e-53 186
MS.gene072817.t1 MTR_6g004220 33.934 333 205 6 1 325 57 382 1.35e-52 182
MS.gene072817.t1 MTR_5g090290 31.567 434 285 5 1 429 73 499 1.26e-51 182
MS.gene072817.t1 MTR_6g047640 27.934 426 286 2 2 427 59 463 2.03e-51 180
MS.gene072817.t1 MTR_5g032750 29.540 413 289 2 2 413 64 475 6.48e-51 179
MS.gene072817.t1 MTR_7g087410 27.674 430 302 3 3 428 60 484 8.89e-51 179
MS.gene072817.t1 MTR_7g087400 27.378 431 303 4 3 429 59 483 4.52e-50 176
MS.gene072817.t1 MTR_1g100180 28.986 414 284 4 2 411 71 478 1.02e-49 176
MS.gene072817.t1 MTR_6g047640 28.404 426 303 1 2 427 59 482 1.10e-49 176
MS.gene072817.t1 MTR_5g033100 27.126 435 302 5 2 429 84 510 1.73e-49 176
MS.gene072817.t1 MTR_6g047590 28.293 410 291 2 2 411 59 465 1.85e-48 172
MS.gene072817.t1 MTR_7g087410 26.768 396 289 1 33 428 1 395 9.26e-48 168
MS.gene072817.t1 MTR_3g099700 27.739 429 302 5 3 427 75 499 1.07e-45 165
MS.gene072817.t1 MTR_7g087430 27.089 395 287 1 33 427 1 394 2.16e-43 157
MS.gene072817.t1 MTR_6g027200 25.629 437 304 3 3 429 65 490 7.61e-42 154
MS.gene072817.t1 MTR_6g004220 34.701 268 166 4 1 264 57 319 1.66e-41 150
MS.gene072817.t1 MTR_7g087370 25.290 431 283 5 3 429 59 454 1.69e-40 150
MS.gene072817.t1 MTR_3g099720 24.645 422 317 1 3 424 70 490 3.06e-40 150
MS.gene072817.t1 MTR_6g027190 26.087 414 284 3 24 428 77 477 1.06e-39 149
MS.gene072817.t1 MTR_2g078990 31.190 311 200 6 118 424 4 304 7.96e-37 137
MS.gene072817.t1 MTR_5g087930 27.064 436 285 6 1 427 53 464 1.10e-36 140
MS.gene072817.t1 MTR_5g090280 26.887 424 267 6 36 428 4 415 1.35e-26 111
MS.gene072817.t1 MTR_2g079030 35.602 191 120 3 213 402 319 507 1.14e-24 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072817.t1 AT4G23030 71.429 427 121 1 1 426 58 484 0.0 586
MS.gene072817.t1 AT1G58340 64.029 417 150 0 1 417 84 500 0.0 526
MS.gene072817.t1 AT4G29140 58.491 424 176 0 1 424 82 505 0.0 523
MS.gene072817.t1 AT5G19700 57.346 422 180 0 1 422 62 483 6.15e-179 509
MS.gene072817.t1 AT5G52050 57.798 436 173 3 3 428 63 497 3.66e-176 502
MS.gene072817.t1 AT2G38510 50.117 429 213 1 1 428 32 460 3.16e-158 456
MS.gene072817.t1 AT1G71870 48.546 447 212 3 1 429 50 496 1.46e-147 429
MS.gene072817.t1 AT5G49130 47.442 430 212 2 4 419 55 484 1.04e-129 384
MS.gene072817.t1 AT4G22790 41.648 437 235 10 2 428 57 483 1.11e-88 278
MS.gene072817.t1 AT5G52450 35.731 431 263 5 1 424 52 475 7.02e-78 250
MS.gene072817.t1 AT2G34360 34.419 430 264 5 1 424 53 470 7.77e-77 247
MS.gene072817.t1 AT1G71140 33.491 424 272 5 1 419 52 470 1.83e-76 246
MS.gene072817.t1 AT1G73700 34.884 430 273 3 1 426 50 476 1.99e-75 243
MS.gene072817.t1 AT2G04070 34.845 419 268 3 5 420 57 473 1.70e-74 241
MS.gene072817.t1 AT2G04080 34.140 413 266 2 5 414 57 466 3.42e-74 240
MS.gene072817.t1 AT2G04050 35.238 420 265 3 5 420 57 473 2.45e-73 238
MS.gene072817.t1 AT1G15150 31.395 430 291 2 1 428 57 484 7.34e-73 237
MS.gene072817.t1 AT1G15160 32.326 430 287 2 1 428 57 484 8.89e-73 236
MS.gene072817.t1 AT1G15170 34.368 419 265 4 1 414 60 473 1.22e-72 236
MS.gene072817.t1 AT2G04080 33.741 409 265 2 5 410 57 462 7.44e-71 231
MS.gene072817.t1 AT2G04040 32.304 421 279 2 5 422 57 474 8.86e-71 231
MS.gene072817.t1 AT1G61890 31.148 427 288 3 2 426 74 496 8.40e-69 226
MS.gene072817.t1 AT2G04090 32.688 413 272 2 5 414 60 469 2.14e-68 225
MS.gene072817.t1 AT1G66760 33.967 421 262 6 2 414 55 467 2.81e-68 224
MS.gene072817.t1 AT2G04100 32.446 413 273 2 5 414 60 469 2.13e-67 222
MS.gene072817.t1 AT1G64820 32.134 417 277 2 1 414 55 468 1.08e-66 221
MS.gene072817.t1 AT1G66780 32.289 415 271 4 5 414 65 474 1.79e-66 220
MS.gene072817.t1 AT1G66760 33.735 415 259 6 2 408 55 461 8.14e-66 218
MS.gene072817.t1 AT1G15180 33.333 417 272 2 1 414 61 474 1.47e-65 218
MS.gene072817.t1 AT1G11670 29.880 415 287 2 2 414 77 489 1.90e-65 218
MS.gene072817.t1 AT1G61890 31.051 409 278 2 2 408 74 480 1.96e-65 218
MS.gene072817.t1 AT3G23550 33.171 410 268 2 1 407 57 463 4.02e-65 216
MS.gene072817.t1 AT2G04100 32.274 409 271 2 5 410 60 465 5.49e-65 216
MS.gene072817.t1 AT2G34360 31.148 427 240 3 1 424 53 428 5.24e-64 212
MS.gene072817.t1 AT3G23560 34.359 390 248 4 1 386 65 450 1.23e-61 207
MS.gene072817.t1 AT4G21910 29.551 423 296 2 2 423 82 503 1.83e-61 207
MS.gene072817.t1 AT4G21910 29.551 423 296 2 2 423 82 503 1.83e-61 207
MS.gene072817.t1 AT1G15170 34.097 349 220 4 71 414 11 354 1.19e-60 201
MS.gene072817.t1 AT4G21910 29.078 423 298 2 2 423 84 505 4.43e-60 204
MS.gene072817.t1 AT4G21910 29.078 423 298 2 2 423 84 505 4.43e-60 204
MS.gene072817.t1 AT3G59030 30.070 429 292 2 1 425 77 501 5.02e-60 204
MS.gene072817.t1 AT3G21690 27.358 424 308 0 2 425 79 502 6.38e-60 203
MS.gene072817.t1 AT1G61890 30.997 371 256 0 2 372 74 444 1.37e-58 199
MS.gene072817.t1 AT3G26590 27.711 415 295 3 2 414 72 483 1.52e-58 199
MS.gene072817.t1 AT4G21903 28.169 426 304 2 2 426 80 504 1.93e-57 197
MS.gene072817.t1 AT5G38030 28.125 416 290 5 2 413 72 482 1.75e-56 194
MS.gene072817.t1 AT1G15150 30.833 360 245 2 71 428 11 368 2.63e-56 190
MS.gene072817.t1 AT3G26590 27.342 395 282 3 36 428 4 395 4.22e-56 190
MS.gene072817.t1 AT4G21903 28.571 392 279 1 36 426 4 395 2.26e-55 188
MS.gene072817.t1 AT4G00350 27.869 427 306 1 2 428 115 539 4.08e-55 191
MS.gene072817.t1 AT4G21903 27.764 407 293 1 2 408 80 485 2.91e-54 189
MS.gene072817.t1 AT1G23300 29.330 433 287 7 5 428 74 496 7.90e-54 187
MS.gene072817.t1 AT5G44050 31.116 421 270 8 3 413 68 478 1.69e-52 183
MS.gene072817.t1 AT1G12950 27.685 419 290 5 2 414 94 505 4.56e-52 183
MS.gene072817.t1 AT1G47530 26.683 416 296 5 3 414 63 473 1.60e-51 181
MS.gene072817.t1 AT3G23560 34.277 318 202 3 1 315 65 378 1.81e-50 176
MS.gene072817.t1 AT5G10420 30.374 428 286 5 3 422 66 489 2.55e-50 177
MS.gene072817.t1 AT4G25640 29.508 427 295 2 2 424 61 485 3.71e-50 177
MS.gene072817.t1 AT4G25640 29.508 427 295 2 2 424 61 485 6.32e-50 177
MS.gene072817.t1 AT5G65380 30.714 420 273 8 3 413 65 475 2.34e-48 172
MS.gene072817.t1 AT1G66760 32.464 345 221 4 2 340 55 393 4.16e-48 169
MS.gene072817.t1 AT1G33090 30.385 441 281 9 3 427 64 494 7.36e-48 171
MS.gene072817.t1 AT1G33100 29.032 434 296 4 3 427 61 491 1.17e-47 170
MS.gene072817.t1 AT1G47530 26.440 382 272 5 37 414 5 381 3.90e-47 166
MS.gene072817.t1 AT1G33080 27.273 429 303 4 3 425 64 489 9.63e-45 162
MS.gene072817.t1 AT1G33080 27.603 413 294 3 3 413 64 473 1.17e-44 162
MS.gene072817.t1 AT1G33110 28.440 436 296 7 3 427 64 494 1.28e-44 162
MS.gene072817.t1 AT3G59030 31.395 344 228 2 1 340 77 416 3.91e-44 159
MS.gene072817.t1 AT1G66760 31.563 339 216 6 78 408 3 333 5.48e-43 154
MS.gene072817.t1 AT4G25640 29.333 375 263 1 2 376 61 433 4.85e-42 154
MS.gene072817.t1 AT5G17700 27.143 420 289 8 3 414 62 472 1.30e-41 154
MS.gene072817.t1 AT5G17700 27.143 420 289 8 3 414 62 472 1.30e-41 154
MS.gene072817.t1 AT3G03620 26.498 434 304 7 3 429 65 490 3.00e-40 150
MS.gene072817.t1 AT3G03620 26.498 434 304 7 3 429 65 490 3.00e-40 150
MS.gene072817.t1 AT1G15180 32.308 260 170 2 71 327 11 267 1.47e-34 133
MS.gene072817.t1 AT1G33110 28.446 341 239 3 3 341 64 401 2.66e-30 121
MS.gene072817.t1 AT5G17700 27.393 303 207 6 3 298 62 358 3.42e-26 109
MS.gene072817.t1 AT2G04066 32.258 124 82 1 291 414 40 161 3.52e-14 70.9

Find 80 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTTACATTTGGGTTTC+TGG 0.044651 5.3:+4337580 MS.gene072817:CDS
AGGTTTGTGGTTTGGTCTTA+TGG 0.195458 5.3:+4338200 MS.gene072817:CDS
TTTCCTTCTTCTTTGAGTTT+TGG 0.208924 5.3:+4337814 MS.gene072817:CDS
TTAGGCGCAAATATAAATTT+AGG 0.214567 5.3:+4338111 MS.gene072817:CDS
CCTATTTGTGGTCAAGCTTT+TGG 0.243633 5.3:+4337178 MS.gene072817:CDS
TTGACAAGAAAATAGTTTAT+AGG 0.246031 5.3:-4337484 None:intergenic
GGTTACAATGTTGTTTGTTT+TGG 0.270469 5.3:+4338242 MS.gene072817:CDS
TTTCGCTGATTATTTACATT+TGG 0.272638 5.3:+4337572 MS.gene072817:CDS
ACATGCAACTGTTGCATCTA+TGG 0.280637 5.3:+4337756 MS.gene072817:CDS
TTCGCTGATTATTTACATTT+GGG 0.298088 5.3:+4337573 MS.gene072817:CDS
TTTATATTTGCGCCTAATTT+CGG 0.301593 5.3:-4338105 None:intergenic
TCTTGTCAATGTTCTTCAAT+TGG 0.313344 5.3:+4337498 MS.gene072817:CDS
ATTGGATTTGCAAACATAAC+AGG 0.318839 5.3:+4337118 MS.gene072817:CDS
GGAATAAGAGGAATCGCTTT+AGG 0.319468 5.3:+4337520 MS.gene072817:CDS
AAAGTAGCACTGTATGTTAA+AGG 0.335720 5.3:-4337442 None:intergenic
CTTGTCAATGTTCTTCAATT+GGG 0.346715 5.3:+4337499 MS.gene072817:CDS
GTTTGGTTGAGTTTCTTTGC+TGG 0.349890 5.3:+4338165 MS.gene072817:CDS
GATTTCAAAGGTTTGTGGTT+TGG 0.352902 5.3:+4338192 MS.gene072817:CDS
TCAATGGTGTTACCGATTAT+AGG 0.360217 5.3:+4338018 MS.gene072817:CDS
GTTCTTCAATTGGGAATAAG+AGG 0.367653 5.3:+4337508 MS.gene072817:CDS
CCAAAAGCTTGACCACAAAT+AGG 0.369074 5.3:-4337178 None:intergenic
CGTTTGCTATATCTTCTTGT+TGG 0.374870 5.3:-4337321 None:intergenic
ATCATTTCAATGTTGTTCCT+TGG 0.403969 5.3:+4337055 None:intergenic
CCTCTGCTTGTTTCAAAGGA+TGG 0.406928 5.3:+4337632 MS.gene072817:CDS
AATGATTGTGCTACAAGATC+AGG 0.410062 5.3:-4337373 None:intergenic
AGCAATTCCAAGCTGCATTT+CGG 0.410449 5.3:+4337672 MS.gene072817:CDS
CTCTGGTCTTGCCATGGGAA+TGG 0.412093 5.3:+4337153 MS.gene072817:CDS
AATAATTGCTTTAACATCAA+TGG 0.417803 5.3:+4338002 MS.gene072817:CDS
ATTTCCTCTGCTTGTTTCAA+AGG 0.418055 5.3:+4337628 MS.gene072817:CDS
GAATCGCTTTAGGTTCCGTT+TGG 0.418185 5.3:+4337530 MS.gene072817:CDS
GCTAAAAGATTCAAACTCCT+TGG 0.419870 5.3:+4337202 MS.gene072817:CDS
GGAACGGCGAGGCCGAAATT+AGG 0.422373 5.3:+4338093 MS.gene072817:CDS
AAACTCAACCAAACTGCAAC+AGG 0.430260 5.3:-4338156 None:intergenic
AGCTCACATAGTCCTATAAT+CGG 0.440906 5.3:-4338030 None:intergenic
GTATGTTAAAGGAAGTGTTA+TGG 0.441085 5.3:-4337431 None:intergenic
GCAGGTGGGTCACTTGCCAT+TGG 0.441197 5.3:+4337100 MS.gene072817:CDS
CATTCTCTCTGGTCTTGCCA+TGG 0.443498 5.3:+4337147 MS.gene072817:CDS
GCAAAACTTGCAGCAATAGT+TGG 0.450295 5.3:+4337886 MS.gene072817:CDS
TTTGGTGTTTCAACAAGAGT+TGG 0.450548 5.3:+4337832 MS.gene072817:CDS
GATGTTAAAGCAATTATTTG+TGG 0.453850 5.3:-4337997 None:intergenic
TGGGAATGCCTGTTGCAGTT+TGG 0.457141 5.3:+4338148 MS.gene072817:CDS
GTTGGTGAGCTTGCTTTAGC+AGG 0.461842 5.3:+4337082 MS.gene072817:CDS
TTTGCTGGATTTGATTTCAA+AGG 0.464102 5.3:+4338180 MS.gene072817:CDS
GATTTGATTTCAAAGGTTTG+TGG 0.465351 5.3:+4338187 MS.gene072817:CDS
ATTCTCTCTGGTCTTGCCAT+GGG 0.465845 5.3:+4337148 MS.gene072817:CDS
GGCTGCTCAAGGTTCATGTA+TGG 0.465974 5.3:+4338221 MS.gene072817:CDS
TTAAAGGAAGTGTTATGGAT+TGG 0.467103 5.3:-4337426 None:intergenic
CGGTTTGTCTCGAATGGTGG+TGG 0.469476 5.3:+4337692 MS.gene072817:CDS
CATGGCAAGACCAGAGAGAA+TGG 0.471209 5.3:-4337146 None:intergenic
GCATTTCGGTTTGTCTCGAA+TGG 0.473600 5.3:+4337686 MS.gene072817:CDS
CCAAATGTTTCTAACAGAAA+AGG 0.479393 5.3:-4337953 None:intergenic
TTCAAGTGAAGAAGAACAAG+AGG 0.495983 5.3:+4338320 MS.gene072817:CDS
ACAGGTTACTCCATTCTCTC+TGG 0.501242 5.3:+4337136 MS.gene072817:CDS
TTTGGTCTTATGGCTGCTCA+AGG 0.501334 5.3:+4338210 MS.gene072817:CDS
ACACCACAAACAGTTGTTTG+TGG 0.502053 5.3:-4338063 None:intergenic
GTGAGCTTGCTTTAGCAGGT+GGG 0.506427 5.3:+4337086 MS.gene072817:CDS
ACAAGAGTTGGAAATGAACT+TGG 0.513291 5.3:+4337844 MS.gene072817:CDS
ATTATAGGACTATGTGAGCT+TGG 0.533869 5.3:+4338033 MS.gene072817:CDS
CTTCGAAGATAGATTCTCAA+AGG 0.539810 5.3:-4337403 None:intergenic
CATGCAACTGTTGCATCTAT+GGG 0.545154 5.3:+4337757 MS.gene072817:CDS
TGTCCACAAACAACTGTTTG+TGG 0.548402 5.3:+4338060 MS.gene072817:CDS
ACACCAAAACTCAAAGAAGA+AGG 0.550180 5.3:-4337817 None:intergenic
AGACAAACCGAAATGCAGCT+TGG 0.556967 5.3:-4337679 None:intergenic
TTCTTTGCATTGTTAAACCA+AGG 0.562087 5.3:-4337219 None:intergenic
GGTGAGCTTGCTTTAGCAGG+TGG 0.562489 5.3:+4337085 MS.gene072817:CDS
CCATCCTTTGAAACAAGCAG+AGG 0.563274 5.3:-4337632 None:intergenic
ATGTTGTTCCTTGGTCGTGT+TGG 0.573577 5.3:+4337064 MS.gene072817:CDS
CTGGAACACACAAGAAAACA+TGG 0.574303 5.3:+4337599 MS.gene072817:CDS
TGTTATGTTTGCAAATCCAA+TGG 0.576457 5.3:-4337116 None:intergenic
TTCGAAGATAGATTCTCAAA+GGG 0.581553 5.3:-4337402 None:intergenic
GAGGTTGAAATTCGTCCAAA+CGG 0.590488 5.3:-4337545 None:intergenic
TTGTGGATCACTTGTGAACA+TGG 0.604296 5.3:-4337980 None:intergenic
TTTCGGTTTGTCTCGAATGG+TGG 0.608886 5.3:+4337689 MS.gene072817:CDS
ATGGGAATGGAACCTATTTG+TGG 0.610601 5.3:+4337166 MS.gene072817:CDS
AAATCAAGCAGAGAGAGCTA+AGG 0.614779 5.3:+4338281 MS.gene072817:CDS
ACAAATAGGTTCCATTCCCA+TGG 0.616628 5.3:-4337164 None:intergenic
ACACACAAGAAAACATGGTG+TGG 0.622763 5.3:+4337604 MS.gene072817:CDS
CAAGCTCACCAACACGACCA+AGG 0.635498 5.3:-4337072 None:intergenic
ATAGATGCAACAGTTGCATG+TGG 0.665559 5.3:-4337754 None:intergenic
AAATAATCAGCGAAACAACG+AGG 0.748054 5.3:-4337564 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTGCTTTAACATCAA+TGG + chr5.3:4338002-4338021 MS.gene072817:CDS 20.0%
!! TTGACAAGAAAATAGTTTAT+AGG - chr5.3:4337487-4337506 None:intergenic 20.0%
!!! CAATTTTCATTTCATTCTTA+TGG + chr5.3:4337266-4337285 MS.gene072817:CDS 20.0%
! AAAAATGTATATCAATGCAG+TGG - chr5.3:4337797-4337816 None:intergenic 25.0%
! GATGTTAAAGCAATTATTTG+TGG - chr5.3:4338000-4338019 None:intergenic 25.0%
! TTAGGCGCAAATATAAATTT+AGG + chr5.3:4338111-4338130 MS.gene072817:CDS 25.0%
! TTTATATTTGCGCCTAATTT+CGG - chr5.3:4338108-4338127 None:intergenic 25.0%
!! AAATCATGATTTTGCTTTGT+GGG + chr5.3:4337719-4337738 MS.gene072817:CDS 25.0%
!! ATTATTTACATTTGGGTTTC+TGG + chr5.3:4337580-4337599 MS.gene072817:CDS 25.0%
!! CTTTTCTGTTAGAAACATTT+GGG + chr5.3:4337954-4337973 MS.gene072817:CDS 25.0%
!! TTCGCTGATTATTTACATTT+GGG + chr5.3:4337573-4337592 MS.gene072817:CDS 25.0%
!! TTTCGCTGATTATTTACATT+TGG + chr5.3:4337572-4337591 MS.gene072817:CDS 25.0%
!!! AAGAGATTATTGCTTTTATG+TGG + chr5.3:4337298-4337317 MS.gene072817:CDS 25.0%
!!! TTAGGTTGTTTTTATCTTGT+GGG + chr5.3:4338129-4338148 MS.gene072817:CDS 25.0%
!!! TTTAGGTTGTTTTTATCTTG+TGG + chr5.3:4338128-4338147 MS.gene072817:CDS 25.0%
AAAGTAGCACTGTATGTTAA+AGG - chr5.3:4337445-4337464 None:intergenic 30.0%
ATTGGATTTGCAAACATAAC+AGG + chr5.3:4337118-4337137 MS.gene072817:CDS 30.0%
CTTGTCAATGTTCTTCAATT+GGG + chr5.3:4337499-4337518 MS.gene072817:CDS 30.0%
TCTTGTCAATGTTCTTCAAT+TGG + chr5.3:4337498-4337517 MS.gene072817:CDS 30.0%
TGTTATGTTTGCAAATCCAA+TGG - chr5.3:4337119-4337138 None:intergenic 30.0%
TTCGAAGATAGATTCTCAAA+GGG - chr5.3:4337405-4337424 None:intergenic 30.0%
TTCTTTGCATTGTTAAACCA+AGG - chr5.3:4337222-4337241 None:intergenic 30.0%
! CCAAATGTTTCTAACAGAAA+AGG - chr5.3:4337956-4337975 None:intergenic 30.0%
! CCTTTTCTGTTAGAAACATT+TGG + chr5.3:4337953-4337972 MS.gene072817:CDS 30.0%
! GAAATCATGATTTTGCTTTG+TGG + chr5.3:4337718-4337737 MS.gene072817:CDS 30.0%
! GATTTGATTTCAAAGGTTTG+TGG + chr5.3:4338187-4338206 MS.gene072817:CDS 30.0%
! TTTGCTGGATTTGATTTCAA+AGG + chr5.3:4338180-4338199 MS.gene072817:CDS 30.0%
!! GGTTACAATGTTGTTTGTTT+TGG + chr5.3:4338242-4338261 MS.gene072817:CDS 30.0%
!! GTATGTTAAAGGAAGTGTTA+TGG - chr5.3:4337434-4337453 None:intergenic 30.0%
!! TTAAAGGAAGTGTTATGGAT+TGG - chr5.3:4337429-4337448 None:intergenic 30.0%
!! TTTCCTTCTTCTTTGAGTTT+TGG + chr5.3:4337814-4337833 MS.gene072817:CDS 30.0%
!!! ACTCTGTTTCAGTTTTGTTT+TGG + chr5.3:4337909-4337928 MS.gene072817:CDS 30.0%
!!! CTCTGTTTCAGTTTTGTTTT+GGG + chr5.3:4337910-4337929 MS.gene072817:CDS 30.0%
!!! TGTTTTGGTTCGTACAAATT+GGG + chr5.3:4338257-4338276 MS.gene072817:CDS 30.0%
!!! TTGTTTTGGTTCGTACAAAT+TGG + chr5.3:4338256-4338275 MS.gene072817:CDS 30.0%
AAATAATCAGCGAAACAACG+AGG - chr5.3:4337567-4337586 None:intergenic 35.0%
AATGATTGTGCTACAAGATC+AGG - chr5.3:4337376-4337395 None:intergenic 35.0%
ACAAGAGTTGGAAATGAACT+TGG + chr5.3:4337844-4337863 MS.gene072817:CDS 35.0%
ACACCAAAACTCAAAGAAGA+AGG - chr5.3:4337820-4337839 None:intergenic 35.0%
AGAAAAGGCGAAAAACAAAG+CGG - chr5.3:4337941-4337960 None:intergenic 35.0%
AGCTCACATAGTCCTATAAT+CGG - chr5.3:4338033-4338052 None:intergenic 35.0%
ATTATAGGACTATGTGAGCT+TGG + chr5.3:4338033-4338052 MS.gene072817:CDS 35.0%
ATTTCCTCTGCTTGTTTCAA+AGG + chr5.3:4337628-4337647 MS.gene072817:CDS 35.0%
CTTCGAAGATAGATTCTCAA+AGG - chr5.3:4337406-4337425 None:intergenic 35.0%
GATTTCAAAGGTTTGTGGTT+TGG + chr5.3:4338192-4338211 MS.gene072817:CDS 35.0%
GCTAAAAGATTCAAACTCCT+TGG + chr5.3:4337202-4337221 MS.gene072817:CDS 35.0%
GTTCTTCAATTGGGAATAAG+AGG + chr5.3:4337508-4337527 MS.gene072817:CDS 35.0%
TTCAAGTGAAGAAGAACAAG+AGG + chr5.3:4338320-4338339 MS.gene072817:CDS 35.0%
!! AACTGTTTGTGGTGTTTTGA+GGG + chr5.3:4338071-4338090 MS.gene072817:CDS 35.0%
!! CGTTTGCTATATCTTCTTGT+TGG - chr5.3:4337324-4337343 None:intergenic 35.0%
!! TCAATGGTGTTACCGATTAT+AGG + chr5.3:4338018-4338037 MS.gene072817:CDS 35.0%
!! TTTGGTGTTTCAACAAGAGT+TGG + chr5.3:4337832-4337851 MS.gene072817:CDS 35.0%
!!! CTGCAAGTTTTGCTTTTTGT+GGG - chr5.3:4337879-4337898 None:intergenic 35.0%
AAACTCAACCAAACTGCAAC+AGG - chr5.3:4338159-4338178 None:intergenic 40.0%
AAATCAAGCAGAGAGAGCTA+AGG + chr5.3:4338281-4338300 MS.gene072817:CDS 40.0%
ACAAATAGGTTCCATTCCCA+TGG - chr5.3:4337167-4337186 None:intergenic 40.0%
ACACACAAGAAAACATGGTG+TGG + chr5.3:4337604-4337623 MS.gene072817:CDS 40.0%
ACACCACAAACAGTTGTTTG+TGG - chr5.3:4338066-4338085 None:intergenic 40.0%
ACATGCAACTGTTGCATCTA+TGG + chr5.3:4337756-4337775 MS.gene072817:CDS 40.0%
AGCAATTCCAAGCTGCATTT+CGG + chr5.3:4337672-4337691 MS.gene072817:CDS 40.0%
ATAGATGCAACAGTTGCATG+TGG - chr5.3:4337757-4337776 None:intergenic 40.0%
ATGGGAATGGAACCTATTTG+TGG + chr5.3:4337166-4337185 MS.gene072817:CDS 40.0%
CATGCAACTGTTGCATCTAT+GGG + chr5.3:4337757-4337776 MS.gene072817:CDS 40.0%
CCAAAAGCTTGACCACAAAT+AGG - chr5.3:4337181-4337200 None:intergenic 40.0%
CTGGAACACACAAGAAAACA+TGG + chr5.3:4337599-4337618 MS.gene072817:CDS 40.0%
GAGGTTGAAATTCGTCCAAA+CGG - chr5.3:4337548-4337567 None:intergenic 40.0%
GCAAAACTTGCAGCAATAGT+TGG + chr5.3:4337886-4337905 MS.gene072817:CDS 40.0%
GGAATAAGAGGAATCGCTTT+AGG + chr5.3:4337520-4337539 MS.gene072817:CDS 40.0%
GTTTGGTTGAGTTTCTTTGC+TGG + chr5.3:4338165-4338184 MS.gene072817:CDS 40.0%
TGTCCACAAACAACTGTTTG+TGG + chr5.3:4338060-4338079 MS.gene072817:CDS 40.0%
TTGTGGATCACTTGTGAACA+TGG - chr5.3:4337983-4338002 None:intergenic 40.0%
! CAACTGTTTGTGGTGTTTTG+AGG + chr5.3:4338070-4338089 MS.gene072817:CDS 40.0%
! CCTATTTGTGGTCAAGCTTT+TGG + chr5.3:4337178-4337197 MS.gene072817:CDS 40.0%
!! ACTGTTTGTGGTGTTTTGAG+GGG + chr5.3:4338072-4338091 MS.gene072817:CDS 40.0%
!! AGGTTTGTGGTTTGGTCTTA+TGG + chr5.3:4338200-4338219 MS.gene072817:CDS 40.0%
!!! GCTGCAAGTTTTGCTTTTTG+TGG - chr5.3:4337880-4337899 None:intergenic 40.0%
ACAGGTTACTCCATTCTCTC+TGG + chr5.3:4337136-4337155 MS.gene072817:CDS 45.0%
AGACAAACCGAAATGCAGCT+TGG - chr5.3:4337682-4337701 None:intergenic 45.0%
ATGTTGTTCCTTGGTCGTGT+TGG + chr5.3:4337064-4337083 MS.gene072817:CDS 45.0%
CCTCTGCTTGTTTCAAAGGA+TGG + chr5.3:4337632-4337651 MS.gene072817:CDS 45.0%
! GAATCGCTTTAGGTTCCGTT+TGG + chr5.3:4337530-4337549 MS.gene072817:CDS 45.0%
! GCATTTCGGTTTGTCTCGAA+TGG + chr5.3:4337686-4337705 MS.gene072817:CDS 45.0%
! TTTCGGTTTGTCTCGAATGG+TGG + chr5.3:4337689-4337708 MS.gene072817:CDS 45.0%
!! ATTCTCTCTGGTCTTGCCAT+GGG + chr5.3:4337148-4337167 MS.gene072817:CDS 45.0%
!! CCATCCTTTGAAACAAGCAG+AGG - chr5.3:4337635-4337654 None:intergenic 45.0%
!! TTTGGTCTTATGGCTGCTCA+AGG + chr5.3:4338210-4338229 MS.gene072817:CDS 45.0%
!!! TTGTGGTGTTTTGAGGGGAA+CGG + chr5.3:4338077-4338096 MS.gene072817:CDS 45.0%
CATGGCAAGACCAGAGAGAA+TGG - chr5.3:4337149-4337168 None:intergenic 50.0%
GGCTGCTCAAGGTTCATGTA+TGG + chr5.3:4338221-4338240 MS.gene072817:CDS 50.0%
TGGGAATGCCTGTTGCAGTT+TGG + chr5.3:4338148-4338167 MS.gene072817:CDS 50.0%
! GTGAGCTTGCTTTAGCAGGT+GGG + chr5.3:4337086-4337105 MS.gene072817:CDS 50.0%
! GTTGGTGAGCTTGCTTTAGC+AGG + chr5.3:4337082-4337101 MS.gene072817:CDS 50.0%
!! CATTCTCTCTGGTCTTGCCA+TGG + chr5.3:4337147-4337166 MS.gene072817:CDS 50.0%
CAAGCTCACCAACACGACCA+AGG - chr5.3:4337075-4337094 None:intergenic 55.0%
CGGTTTGTCTCGAATGGTGG+TGG + chr5.3:4337692-4337711 MS.gene072817:CDS 55.0%
! GGTGAGCTTGCTTTAGCAGG+TGG + chr5.3:4337085-4337104 MS.gene072817:CDS 55.0%
!! CTCTGGTCTTGCCATGGGAA+TGG + chr5.3:4337153-4337172 MS.gene072817:CDS 55.0%
GCAGGTGGGTCACTTGCCAT+TGG + chr5.3:4337100-4337119 MS.gene072817:CDS 60.0%
GGAACGGCGAGGCCGAAATT+AGG + chr5.3:4338093-4338112 MS.gene072817:CDS 60.0%
!!! GTGTTTTGAGGGGAACGGCG+AGG + chr5.3:4338082-4338101 MS.gene072817:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 4337064 4338350 4337064 ID=MS.gene072817
chr5.3 mRNA 4337064 4338350 4337064 ID=MS.gene072817.t1;Parent=MS.gene072817
chr5.3 exon 4337064 4338350 4337064 ID=MS.gene072817.t1.exon1;Parent=MS.gene072817.t1
chr5.3 CDS 4337064 4338350 4337064 ID=cds.MS.gene072817.t1;Parent=MS.gene072817.t1
Gene Sequence

>MS.gene072817

ATGTTGTTCCTTGGTCGTGTTGGTGAGCTTGCTTTAGCAGGTGGGTCACTTGCCATTGGATTTGCAAACATAACAGGTTACTCCATTCTCTCTGGTCTTGCCATGGGAATGGAACCTATTTGTGGTCAAGCTTTTGGTGCTAAAAGATTCAAACTCCTTGGTTTAACAATGCAAAGAACAGTGATTCTTCTTCTTGTAACTTCAATTTTCATTTCATTCTTATGGCTTAACATGAAGAGATTATTGCTTTTATGTGGCCAACAAGAAGATATAGCAAACGTTGCACAATCTTACATTCTTTATTCTCTTCCTGATCTTGTAGCACAATCATTGCTACACCCTTTGAGAATCTATCTTCGAAGCCAATCCATAACACTTCCTTTAACATACAGTGCTACTTTGTCTATTCTTCTTCACATTCCTATAAACTATTTTCTTGTCAATGTTCTTCAATTGGGAATAAGAGGAATCGCTTTAGGTTCCGTTTGGACGAATTTCAACCTCGTTGTTTCGCTGATTATTTACATTTGGGTTTCTGGAACACACAAGAAAACATGGTGTGGAATTTCCTCTGCTTGTTTCAAAGGATGGAAATCGCTTTTGAATTTAGCAATTCCAAGCTGCATTTCGGTTTGTCTCGAATGGTGGTGGTACGAAATCATGATTTTGCTTTGTGGGTTATTGCTTAATCCACATGCAACTGTTGCATCTATGGGTGTGTTGATTCAAACCACTGCATTGATATACATTTTTCCTTCTTCTTTGAGTTTTGGTGTTTCAACAAGAGTTGGAAATGAACTTGGTGCTGAAAACCCACAAAAAGCAAAACTTGCAGCAATAGTTGGACTCTGTTTCAGTTTTGTTTTGGGTTTCTCCGCTTTGTTTTTCGCCTTTTCTGTTAGAAACATTTGGGCTACCATGTTCACAAGTGATCCACAAATAATTGCTTTAACATCAATGGTGTTACCGATTATAGGACTATGTGAGCTTGGAAATTGTCCACAAACAACTGTTTGTGGTGTTTTGAGGGGAACGGCGAGGCCGAAATTAGGCGCAAATATAAATTTAGGTTGTTTTTATCTTGTGGGAATGCCTGTTGCAGTTTGGTTGAGTTTCTTTGCTGGATTTGATTTCAAAGGTTTGTGGTTTGGTCTTATGGCTGCTCAAGGTTCATGTATGGTTACAATGTTGTTTGTTTTGGTTCGTACAAATTGGGAAAATCAAGCAGAGAGAGCTAAGGAGTTAACATCATCAGATTCAAGTGAAGAAGAACAAGAGGAAGAGAAA
Protein sequence

>MS.gene072817.t1

MLFLGRVGELALAGGSLAIGFANITGYSILSGLAMGMEPICGQAFGAKRFKLLGLTMQRTVILLLVTSIFISFLWLNMKRLLLLCGQQEDIANVAQSYILYSLPDLVAQSLLHPLRIYLRSQSITLPLTYSATLSILLHIPINYFLVNVLQLGIRGIALGSVWTNFNLVVSLIIYIWVSGTHKKTWCGISSACFKGWKSLLNLAIPSCISVCLEWWWYEIMILLCGLLLNPHATVASMGVLIQTTALIYIFPSSLSFGVSTRVGNELGAENPQKAKLAAIVGLCFSFVLGFSALFFAFSVRNIWATMFTSDPQIIALTSMVLPIIGLCELGNCPQTTVCGVLRGTARPKLGANINLGCFYLVGMPVAVWLSFFAGFDFKGLWFGLMAAQGSCMVTMLFVLVRTNWENQAERAKELTSSDSSEEEQEEEK