AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49046

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49046.t1 MTR_1g101500 97.792 453 10 0 1 453 88 540 0.0 896
MS.gene49046.t1 MTR_0039s0080 76.327 452 99 1 1 452 62 505 0.0 702
MS.gene49046.t1 MTR_5g010830 76.372 419 99 0 1 419 89 507 0.0 666
MS.gene49046.t1 MTR_5g067460 61.395 430 164 1 1 430 79 506 0.0 562
MS.gene49046.t1 MTR_3g111020 61.806 432 159 1 1 426 75 506 0.0 561
MS.gene49046.t1 MTR_6g088805 54.545 429 192 1 1 429 51 476 1.05e-169 486
MS.gene49046.t1 MTR_8g102430 56.563 419 162 3 1 419 47 445 8.78e-167 477
MS.gene49046.t1 MTR_6g043810 48.908 458 200 5 2 441 48 489 9.20e-156 451
MS.gene49046.t1 MTR_6g043810 48.908 458 200 5 2 441 58 499 9.33e-156 451
MS.gene49046.t1 MTR_4g027920 51.487 437 207 2 1 435 180 613 3.81e-153 448
MS.gene49046.t1 MTR_2g435930 48.307 443 199 3 1 420 43 478 2.56e-137 404
MS.gene49046.t1 MTR_6g081410 35.012 417 269 1 1 415 49 465 3.24e-92 288
MS.gene49046.t1 MTR_6g066380 35.012 417 269 1 1 415 51 467 9.85e-91 284
MS.gene49046.t1 MTR_6g081300 37.590 415 259 0 1 415 34 448 2.03e-90 283
MS.gene49046.t1 MTR_6g081400 36.842 418 263 1 1 417 39 456 2.24e-89 280
MS.gene49046.t1 MTR_8g106670 35.800 419 263 2 2 417 37 452 1.50e-88 278
MS.gene49046.t1 MTR_6g081780 34.053 417 273 1 1 415 49 465 2.95e-88 277
MS.gene49046.t1 MTR_6g081240 35.990 414 264 1 1 413 33 446 2.54e-87 274
MS.gene49046.t1 MTR_6g081810 33.732 418 245 3 1 415 51 439 1.36e-85 270
MS.gene49046.t1 MTR_5g011540 39.101 445 261 5 2 442 68 506 1.22e-84 269
MS.gene49046.t1 MTR_6g081310 36.092 435 265 3 1 422 20 454 5.90e-84 266
MS.gene49046.t1 MTR_1g108810 34.541 414 265 2 1 411 58 468 1.11e-82 263
MS.gene49046.t1 MTR_1g108840 33.886 422 269 4 1 417 65 481 3.38e-78 251
MS.gene49046.t1 MTR_6g081390 33.014 418 279 1 1 417 36 453 4.79e-78 250
MS.gene49046.t1 MTR_6g004220 34.824 425 262 5 1 417 47 464 1.87e-77 249
MS.gene49046.t1 MTR_6g004220 34.845 419 258 5 1 411 54 465 8.18e-75 243
MS.gene49046.t1 MTR_2g078930 33.810 420 272 3 1 417 67 483 5.76e-74 241
MS.gene49046.t1 MTR_7g082810 29.797 443 297 5 1 437 44 478 1.56e-69 229
MS.gene49046.t1 MTR_2g088400 30.435 437 295 1 1 437 76 503 6.04e-69 228
MS.gene49046.t1 MTR_2g078960 32.471 425 277 4 1 420 57 476 4.80e-68 225
MS.gene49046.t1 MTR_4g048000 29.673 428 301 0 1 428 88 515 3.00e-67 224
MS.gene49046.t1 MTR_1g108990 32.464 422 275 4 1 417 49 465 4.38e-66 219
MS.gene49046.t1 MTR_2g079020 32.388 423 274 4 1 417 61 477 6.42e-66 219
MS.gene49046.t1 MTR_2g078970 32.861 423 272 4 1 417 55 471 2.34e-65 218
MS.gene49046.t1 MTR_1g109060 31.190 420 283 2 1 417 48 464 6.33e-65 216
MS.gene49046.t1 MTR_2g022980 29.808 416 292 0 2 417 73 488 2.37e-64 215
MS.gene49046.t1 MTR_1g109060 31.190 420 282 3 1 416 48 464 2.52e-62 210
MS.gene49046.t1 MTR_7g082800 32.134 389 250 4 36 417 1 382 6.38e-62 206
MS.gene49046.t1 MTR_2g088400 31.135 379 261 0 1 379 76 454 1.47e-60 204
MS.gene49046.t1 MTR_8g069470 28.764 445 303 7 2 440 75 511 2.69e-59 202
MS.gene49046.t1 MTR_6g004220 36.012 336 200 5 1 328 54 382 3.73e-59 201
MS.gene49046.t1 MTR_5g090290 30.822 438 300 3 2 437 71 507 1.83e-56 196
MS.gene49046.t1 MTR_5g032720 30.383 418 287 3 1 416 60 475 2.94e-56 194
MS.gene49046.t1 MTR_5g033100 29.384 422 285 5 2 417 81 495 7.04e-56 193
MS.gene49046.t1 MTR_8g063590 30.935 417 283 3 2 415 78 492 1.69e-55 192
MS.gene49046.t1 MTR_1g021740 29.808 416 283 3 6 417 64 474 6.96e-55 190
MS.gene49046.t1 MTR_5g032750 30.383 418 287 3 1 416 60 475 1.00e-54 190
MS.gene49046.t1 MTR_7g087410 29.216 421 289 3 1 417 55 470 6.33e-51 180
MS.gene49046.t1 MTR_6g047610 28.747 407 287 2 5 411 59 462 5.69e-49 174
MS.gene49046.t1 MTR_7g087400 27.594 424 291 4 1 417 54 468 9.31e-49 174
MS.gene49046.t1 MTR_1g100180 27.803 446 307 4 2 445 68 500 3.59e-48 172
MS.gene49046.t1 MTR_6g047640 28.205 429 286 3 3 430 57 464 9.04e-48 171
MS.gene49046.t1 MTR_6g047590 29.238 407 285 2 5 411 59 462 2.80e-47 169
MS.gene49046.t1 MTR_6g004220 36.900 271 162 4 1 267 54 319 1.23e-46 164
MS.gene49046.t1 MTR_7g087410 28.272 382 273 1 36 417 1 381 3.74e-46 165
MS.gene49046.t1 MTR_6g047640 28.671 429 303 2 3 430 57 483 1.17e-45 165
MS.gene49046.t1 MTR_3g099700 28.211 436 299 7 1 429 70 498 3.22e-44 162
MS.gene49046.t1 MTR_7g087430 28.462 390 262 2 36 417 1 381 5.81e-44 159
MS.gene49046.t1 MTR_5g087930 29.820 389 250 4 2 389 51 417 4.55e-43 158
MS.gene49046.t1 MTR_3g099720 25.117 426 317 2 1 426 65 488 8.28e-41 152
MS.gene49046.t1 MTR_6g027190 26.492 419 291 6 1 411 48 457 4.18e-39 147
MS.gene49046.t1 MTR_6g027200 26.128 421 302 3 1 417 60 475 1.54e-38 146
MS.gene49046.t1 MTR_7g087370 25.236 424 272 5 1 417 54 439 1.69e-37 142
MS.gene49046.t1 MTR_2g078990 29.373 303 202 5 121 417 4 300 2.07e-35 134
MS.gene49046.t1 MTR_5g090280 26.214 412 262 6 39 417 4 406 1.38e-26 111
MS.gene49046.t1 MTR_2g079030 29.082 196 133 2 216 408 319 511 4.19e-19 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49046.t1 AT4G23030 68.090 445 138 2 1 441 55 499 0.0 586
MS.gene49046.t1 AT5G52050 57.556 450 181 3 2 445 59 504 0.0 526
MS.gene49046.t1 AT1G58340 61.098 419 163 0 1 419 81 499 8.72e-180 513
MS.gene49046.t1 AT4G29140 57.416 418 178 0 2 419 80 497 2.79e-178 509
MS.gene49046.t1 AT5G19700 58.852 418 172 0 2 419 60 477 7.39e-177 505
MS.gene49046.t1 AT2G38510 47.778 450 234 1 1 449 29 478 3.00e-152 441
MS.gene49046.t1 AT1G71870 46.537 462 224 3 1 439 47 508 7.47e-148 431
MS.gene49046.t1 AT5G49130 47.007 451 225 1 1 437 49 499 2.14e-138 407
MS.gene49046.t1 AT4G22790 41.419 437 248 6 5 436 57 490 1.79e-84 268
MS.gene49046.t1 AT5G52450 35.160 438 282 1 1 438 49 484 2.84e-82 262
MS.gene49046.t1 AT2G34360 33.333 444 275 6 1 438 50 478 2.90e-79 254
MS.gene49046.t1 AT1G71140 34.043 423 267 5 1 417 49 465 5.44e-77 248
MS.gene49046.t1 AT1G66760 33.180 434 283 3 3 433 53 482 5.67e-75 243
MS.gene49046.t1 AT2G04070 35.000 420 267 3 1 417 50 466 6.19e-73 238
MS.gene49046.t1 AT1G73700 33.097 423 277 2 1 420 47 466 2.65e-72 236
MS.gene49046.t1 AT1G15150 32.110 436 282 2 1 429 54 482 3.28e-72 236
MS.gene49046.t1 AT2G04050 34.772 417 272 0 1 417 50 466 3.32e-72 236
MS.gene49046.t1 AT1G66780 33.810 420 272 2 1 417 58 474 5.59e-72 235
MS.gene49046.t1 AT2G04080 33.094 417 279 0 1 417 50 466 7.75e-72 234
MS.gene49046.t1 AT1G15170 32.706 425 286 0 1 425 57 481 1.36e-71 234
MS.gene49046.t1 AT1G66760 33.333 414 270 2 3 413 53 463 1.39e-71 234
MS.gene49046.t1 AT2G04040 34.048 420 271 3 1 417 50 466 7.11e-71 232
MS.gene49046.t1 AT1G15160 31.002 429 296 0 1 429 54 482 1.82e-70 231
MS.gene49046.t1 AT2G04080 33.092 414 276 1 1 414 50 462 1.36e-69 228
MS.gene49046.t1 AT2G04100 32.536 418 280 2 1 417 53 469 1.66e-69 229
MS.gene49046.t1 AT1G64820 31.027 448 293 5 1 438 52 493 5.50e-69 228
MS.gene49046.t1 AT2G04090 32.536 418 280 2 1 417 53 469 1.40e-68 226
MS.gene49046.t1 AT3G23550 34.518 394 248 5 1 389 54 442 2.19e-68 225
MS.gene49046.t1 AT1G61890 29.748 437 297 2 1 437 70 496 1.17e-67 224
MS.gene49046.t1 AT2G04100 32.683 410 274 2 1 409 53 461 1.85e-67 223
MS.gene49046.t1 AT1G61890 30.414 411 286 0 1 411 70 480 7.02e-67 222
MS.gene49046.t1 AT2G34360 30.385 441 250 4 1 438 50 436 1.75e-66 219
MS.gene49046.t1 AT1G15180 32.629 426 285 2 1 425 58 482 3.60e-66 220
MS.gene49046.t1 AT3G23560 34.184 392 248 5 3 389 64 450 1.90e-65 218
MS.gene49046.t1 AT3G26590 29.904 418 288 2 2 417 69 483 2.64e-65 218
MS.gene49046.t1 AT3G21690 31.151 443 292 3 1 441 75 506 2.90e-65 218
MS.gene49046.t1 AT1G11670 28.404 426 305 0 1 426 73 498 5.84e-64 214
MS.gene49046.t1 AT4G21910 30.115 435 293 3 3 437 80 503 9.42e-64 214
MS.gene49046.t1 AT4G21910 30.115 435 293 3 3 437 80 503 9.42e-64 214
MS.gene49046.t1 AT4G21910 30.115 435 293 3 3 437 82 505 2.31e-63 213
MS.gene49046.t1 AT4G21910 30.115 435 293 3 3 437 82 505 2.31e-63 213
MS.gene49046.t1 AT5G38030 30.861 418 280 5 3 416 70 482 1.17e-62 211
MS.gene49046.t1 AT4G21903 29.782 413 289 1 5 417 80 491 1.31e-62 211
MS.gene49046.t1 AT3G26590 30.184 381 261 2 39 417 4 381 4.68e-61 204
MS.gene49046.t1 AT4G21903 29.584 409 287 1 5 413 80 487 8.81e-61 206
MS.gene49046.t1 AT1G61890 30.933 375 259 0 1 375 70 444 3.47e-60 204
MS.gene49046.t1 AT4G21903 29.815 379 266 0 39 417 4 382 1.64e-58 197
MS.gene49046.t1 AT1G12950 28.365 416 297 1 2 417 91 505 6.42e-58 199
MS.gene49046.t1 AT1G15170 31.198 359 247 0 67 425 4 362 2.79e-57 193
MS.gene49046.t1 AT3G59030 29.736 417 291 2 2 417 75 490 1.36e-55 192
MS.gene49046.t1 AT3G23560 34.375 320 203 3 3 319 64 379 1.40e-55 190
MS.gene49046.t1 AT4G00350 29.298 413 290 1 5 417 115 525 1.17e-53 188
MS.gene49046.t1 AT1G66760 34.043 329 211 2 3 328 53 378 1.30e-53 184
MS.gene49046.t1 AT1G15150 30.270 370 244 2 67 429 4 366 7.61e-53 182
MS.gene49046.t1 AT5G44050 29.621 422 285 4 1 416 63 478 2.96e-52 183
MS.gene49046.t1 AT5G10420 29.216 421 288 3 1 416 61 476 1.43e-51 181
MS.gene49046.t1 AT1G33100 29.880 415 282 5 1 411 56 465 1.77e-51 181
MS.gene49046.t1 AT4G25640 29.099 433 299 3 2 430 58 486 1.99e-51 181
MS.gene49046.t1 AT4G25640 29.099 433 299 3 2 430 58 486 3.65e-51 181
MS.gene49046.t1 AT1G33110 30.120 415 281 5 1 411 59 468 1.07e-50 179
MS.gene49046.t1 AT1G33090 30.456 417 277 5 1 411 59 468 3.34e-50 178
MS.gene49046.t1 AT1G23300 26.949 449 315 6 2 442 68 511 7.19e-50 177
MS.gene49046.t1 AT1G33080 28.947 418 292 3 1 416 59 473 1.44e-49 176
MS.gene49046.t1 AT1G33080 29.298 413 287 3 1 411 59 468 3.02e-49 175
MS.gene49046.t1 AT1G47530 26.077 418 304 3 2 417 59 473 2.26e-47 170
MS.gene49046.t1 AT5G65380 28.538 424 287 6 1 416 60 475 3.17e-46 167
MS.gene49046.t1 AT1G66760 29.882 338 231 2 79 413 1 335 2.73e-45 161
MS.gene49046.t1 AT4G25640 28.836 378 267 1 2 379 58 433 4.12e-45 164
MS.gene49046.t1 AT3G59030 30.267 337 235 0 2 338 75 411 6.92e-44 159
MS.gene49046.t1 AT1G47530 26.053 380 276 3 40 417 5 381 2.44e-43 157
MS.gene49046.t1 AT5G17700 24.703 421 308 3 1 417 57 472 4.10e-37 142
MS.gene49046.t1 AT5G17700 24.703 421 308 3 1 417 57 472 4.10e-37 142
MS.gene49046.t1 AT1G33110 29.706 340 234 3 1 338 59 395 9.67e-36 137
MS.gene49046.t1 AT1G15180 32.075 265 178 2 67 330 4 267 1.21e-35 136
MS.gene49046.t1 AT3G03620 25.653 421 298 7 1 414 60 472 5.18e-34 134
MS.gene49046.t1 AT3G03620 25.653 421 298 7 1 414 60 472 5.18e-34 134
MS.gene49046.t1 AT5G17700 26.510 298 210 3 1 294 57 349 9.44e-25 105
MS.gene49046.t1 AT2G04066 30.894 123 82 1 298 417 39 161 6.62e-13 67.0

Find 92 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTAAGGTAAATTCTTAA+AGG 0.188882 1.3:+69919164 None:intergenic
GTAGGTGCAAATATTAATTT+TGG 0.189235 1.3:-69918456 MS.gene49046:CDS
AGAAACAAGAAAATAGTTTA+TGG 0.224806 1.3:+69919082 None:intergenic
AAGAGCAACCGTTGCTTCTA+TGG 0.230580 1.3:-69918811 MS.gene49046:CDS
TAAAGCCAAAGTAACGAAAT+AGG 0.235844 1.3:+69919296 None:intergenic
ATGATCTCCATGCTCTTTCT+TGG 0.239293 1.3:-69919512 MS.gene49046:CDS
GTGTAGCTATATCTTCTTCT+TGG 0.241563 1.3:+69919246 None:intergenic
TCTTTAGTGTTACCTCTAAT+AGG 0.251370 1.3:-69918549 MS.gene49046:CDS
GTTACTTAAAGCAGAAATTA+AGG 0.261872 1.3:-69918187 MS.gene49046:CDS
GCTTGGGTTGCTTGTGGCTC+AGG 0.272940 1.3:-69918358 MS.gene49046:CDS
TTAATATTTGCACCTACTTT+AGG 0.280686 1.3:+69918462 None:intergenic
GTCTTTCTGTTTGCCTTGAA+TGG 0.283276 1.3:-69918881 MS.gene49046:CDS
CTATAATGGTTAGGAACATT+TGG 0.285929 1.3:-69918614 MS.gene49046:CDS
GCTAAGAAATTAACTGGAAT+TGG 0.311846 1.3:-69918270 MS.gene49046:CDS
GAGTAATGGATTGGCTTCTA+AGG 0.325198 1.3:+69919150 None:intergenic
TTGGAATGCCTGTGGCAATT+TGG 0.341824 1.3:-69918419 MS.gene49046:CDS
CCAAAGGCTTGTCCACAAAT+AGG 0.358838 1.3:+69919389 None:intergenic
CTAAGAGCTAAGAAATTAAC+TGG 0.360053 1.3:-69918276 MS.gene49046:CDS
GTCGGCTTCGCGAACATTAC+CGG 0.361524 1.3:-69919449 MS.gene49046:CDS
CTTTAGTGTTACCTCTAATA+GGG 0.382877 1.3:-69918548 MS.gene49046:CDS
CCTATTTGTGGACAAGCCTT+TGG 0.387828 1.3:-69919389 MS.gene49046:CDS
CTAATAGGGCTATGTGAACT+TGG 0.396319 1.3:-69918534 MS.gene49046:CDS
TATAATGGTTAGGAACATTT+GGG 0.400979 1.3:-69918613 MS.gene49046:CDS
ACCCCACAACCTGTTGTTTG+TGG 0.402973 1.3:+69918504 None:intergenic
CGCGAGTGAGCCACCGGCTA+AGG 0.421502 1.3:+69919472 None:intergenic
TTCTAAGGTAAATTCTTAAA+GGG 0.421780 1.3:+69919165 None:intergenic
ATAGAAGCAACGGTTGCTCT+TGG 0.422605 1.3:+69918813 None:intergenic
GCAAAACTTTCAGCTATAGT+AGG 0.427143 1.3:-69918681 MS.gene49046:CDS
ATCAAAGGTGTAGCTTTAAG+TGG 0.427558 1.3:-69919044 MS.gene49046:CDS
GAAACAAGAAAATAGTTTAT+GGG 0.427619 1.3:+69919083 None:intergenic
CTTGGGTTGCTTGTGGCTCA+GGG 0.431311 1.3:-69918357 MS.gene49046:CDS
AGTTAAGAGTAGCAAGAGAA+TGG 0.432962 1.3:+69919325 None:intergenic
TTTCATACCACCACCATTCA+AGG 0.433023 1.3:+69918868 None:intergenic
AGTTCACATAGCCCTATTAG+AGG 0.433783 1.3:+69918537 None:intergenic
ACCGGAGAGGATTGAGTAAC+CGG 0.443135 1.3:+69919430 None:intergenic
ATGCTCTTTCTTGGTCACCT+TGG 0.443187 1.3:-69919503 MS.gene49046:CDS
CTATCCCTATTTCGTTACTT+TGG 0.446430 1.3:-69919301 MS.gene49046:CDS
ACCAAGGAGAGTGAATCTTT+TGG 0.446968 1.3:+69919364 None:intergenic
AAAGATTGTGCTAAGAGATC+AGG 0.449479 1.3:+69919194 None:intergenic
ACACTAAAACTTATTGATGA+TGG 0.453242 1.3:+69918750 None:intergenic
CTGTTTGCCTTGAATGGTGG+TGG 0.453436 1.3:-69918875 MS.gene49046:CDS
GCCAAAAGATTCACTCTCCT+TGG 0.461379 1.3:-69919365 MS.gene49046:CDS
CATCAGAATGTTTCAAACAA+TGG 0.462471 1.3:-69918935 MS.gene49046:CDS
CTTCTTGGCCACATAAAAGA+AGG 0.464872 1.3:+69919261 None:intergenic
CTCCGGTCTCGCTGTAGGAA+TGG 0.471457 1.3:-69919414 MS.gene49046:CDS
GGAAATTGTCCACAAACAAC+AGG 0.473001 1.3:-69918513 MS.gene49046:CDS
CTTGGTGAACTTGCCTTAGC+CGG 0.479237 1.3:-69919485 MS.gene49046:CDS
TTCCATTCCTACAGCGAGAC+CGG 0.489423 1.3:+69919412 None:intergenic
CACCTTTGATTCCCATGTTG+AGG 0.493416 1.3:+69919057 None:intergenic
ACAGGTTGTGGGGTGTTAAG+AGG 0.494385 1.3:-69918495 MS.gene49046:CDS
TTTAGTGTTTCCACTAGAGT+TGG 0.502942 1.3:-69918735 MS.gene49046:CDS
GCCGACCGCGAGTGAGCCAC+CGG 0.507517 1.3:+69919466 None:intergenic
AAAGCCAAAGTAACGAAATA+GGG 0.508997 1.3:+69919297 None:intergenic
CTGGCACACACAAGAAAACA+TGG 0.516093 1.3:-69918968 MS.gene49046:CDS
ACTAGAGTTGGTAACAAACT+TGG 0.522181 1.3:-69918723 MS.gene49046:CDS
GTCCACAAACAACAGGTTGT+GGG 0.527219 1.3:-69918506 MS.gene49046:CDS
ACCGGTTACTCAATCCTCTC+CGG 0.528593 1.3:-69919431 MS.gene49046:CDS
AGAGCAACCGTTGCTTCTAT+GGG 0.530479 1.3:-69918810 MS.gene49046:CDS
TTTCTGTTTGCCTTGAATGG+TGG 0.531083 1.3:-69918878 MS.gene49046:CDS
GTAGGAATGGAACCTATTTG+TGG 0.533221 1.3:-69919401 MS.gene49046:CDS
CACCTCAACATGGGAATCAA+AGG 0.535127 1.3:-69919059 MS.gene49046:CDS
TGTCCACAAACAACAGGTTG+TGG 0.537825 1.3:-69918507 MS.gene49046:CDS
TCTTGTTTCTCACCTCAACA+TGG 0.538883 1.3:-69919069 MS.gene49046:CDS
CTTGTTTCTCACCTCAACAT+GGG 0.546073 1.3:-69919068 MS.gene49046:CDS
TCAAAGGTGTAGCTTTAAGT+GGG 0.553175 1.3:-69919043 MS.gene49046:CDS
TGAGAGGCAGAGTAATGGAT+TGG 0.565370 1.3:+69919141 None:intergenic
AAGTTTGTTACCAACTCTAG+TGG 0.568228 1.3:+69918725 None:intergenic
GGAACAGCAAGGCCTAAAGT+AGG 0.568570 1.3:-69918474 MS.gene49046:CDS
GTGTAGCTTTAAGTGGGGTG+TGG 0.568582 1.3:-69919037 MS.gene49046:CDS
TTGTGCTAAGAGATCAGGAA+TGG 0.570625 1.3:+69919199 None:intergenic
AGGCAAACAGAAAGACAACT+TGG 0.572923 1.3:+69918888 None:intergenic
CAAAATTCCCATAGAAGCAA+CGG 0.574636 1.3:+69918803 None:intergenic
ATCCTCTCCGGTCTCGCTGT+AGG 0.580750 1.3:-69919419 MS.gene49046:CDS
GCCGGTGGCTCACTCGCGGT+CGG 0.585378 1.3:-69919467 MS.gene49046:CDS
TTTGTGGCTTGGGTTGCTTG+TGG 0.588167 1.3:-69918364 MS.gene49046:CDS
AAAGTAGCACAAAGTGTGAG+AGG 0.590144 1.3:+69919125 None:intergenic
GGTGAACTTGCCTTAGCCGG+TGG 0.590167 1.3:-69919482 MS.gene49046:CDS
AAGTGTGAGAGGCAGAGTAA+TGG 0.593415 1.3:+69919136 None:intergenic
AAAGCTAACCAAATTGCCAC+AGG 0.593519 1.3:+69918411 None:intergenic
AACTTGTGCTGTGACTATGT+TGG 0.594620 1.3:-69918334 MS.gene49046:CDS
AAGGTGACCAAGAAAGAGCA+TGG 0.612430 1.3:+69919505 None:intergenic
TCCACAAACAACAGGTTGTG+GGG 0.625297 1.3:-69918505 MS.gene49046:CDS
TTACATTGTTGGAATGCCTG+TGG 0.640132 1.3:-69918427 MS.gene49046:CDS
CTTAGCCGGTGGCTCACTCG+CGG 0.647085 1.3:-69919471 MS.gene49046:CDS
TGGCACACACAAGAAAACAT+GGG 0.651572 1.3:-69918967 MS.gene49046:CDS
ACACACAAGAAAACATGGGG+TGG 0.668171 1.3:-69918963 MS.gene49046:CDS
AGAGAATCAAAGATGCAACA+AGG 0.675363 1.3:+69919003 None:intergenic
CAAAGGTGTAGCTTTAAGTG+GGG 0.681260 1.3:-69919042 MS.gene49046:CDS
TTCCTACAGCGAGACCGGAG+AGG 0.683946 1.3:+69919417 None:intergenic
GGCACACACAAGAAAACATG+GGG 0.684473 1.3:-69918966 MS.gene49046:CDS
GGGTGTTAAGAGGAACAGCA+AGG 0.695898 1.3:-69918485 MS.gene49046:CDS
CGGCTAAGGCAAGTTCACCA+AGG 0.698456 1.3:+69919486 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAACAAGAAAATAGTTTA+TGG + chr1.3:69918606-69918625 None:intergenic 20.0%
!! GAAACAAGAAAATAGTTTAT+GGG + chr1.3:69918605-69918624 None:intergenic 20.0%
!!! TTCTAAGGTAAATTCTTAAA+GGG + chr1.3:69918523-69918542 None:intergenic 20.0%
! ACACTAAAACTTATTGATGA+TGG + chr1.3:69918938-69918957 None:intergenic 25.0%
! GTTACTTAAAGCAGAAATTA+AGG - chr1.3:69919498-69919517 MS.gene49046:CDS 25.0%
! TATAATGGTTAGGAACATTT+GGG - chr1.3:69919072-69919091 MS.gene49046:CDS 25.0%
! TTAATATTTGCACCTACTTT+AGG + chr1.3:69919226-69919245 None:intergenic 25.0%
!! AAACATATCCTTCTTTTATG+TGG - chr1.3:69918416-69918435 MS.gene49046:CDS 25.0%
!! ATTCTCTACATAATTTTCTC+TGG - chr1.3:69918698-69918717 MS.gene49046:CDS 25.0%
!! GTAGGTGCAAATATTAATTT+TGG - chr1.3:69919229-69919248 MS.gene49046:CDS 25.0%
!!! ATTTGGTTAGCTTTTTATGT+TGG - chr1.3:69919283-69919302 MS.gene49046:CDS 25.0%
!!! CTTCTAAGGTAAATTCTTAA+AGG + chr1.3:69918524-69918543 None:intergenic 25.0%
!!! GAAATCATGATTTTGTTATG+TGG - chr1.3:69918836-69918855 MS.gene49046:CDS 25.0%
!!! TGCTTTGTTTTTTGCTATAA+TGG - chr1.3:69919057-69919076 MS.gene49046:CDS 25.0%
!!! TGTTTTTTGCTATAATGGTT+AGG - chr1.3:69919062-69919081 MS.gene49046:CDS 25.0%
!!! TTTGGTTAGCTTTTTATGTT+GGG - chr1.3:69919284-69919303 MS.gene49046:CDS 25.0%
!!! TTTGGTTGTTTTTACATTGT+TGG - chr1.3:69919247-69919266 MS.gene49046:CDS 25.0%
!!! TTTTTTGTAAACATAGACCA+AGG + chr1.3:69918340-69918359 None:intergenic 25.0%
AAAGCCAAAGTAACGAAATA+GGG + chr1.3:69918391-69918410 None:intergenic 30.0%
CATCAGAATGTTTCAAACAA+TGG - chr1.3:69918750-69918769 MS.gene49046:CDS 30.0%
CTAAGAGCTAAGAAATTAAC+TGG - chr1.3:69919409-69919428 MS.gene49046:CDS 30.0%
CTATAATGGTTAGGAACATT+TGG - chr1.3:69919071-69919090 MS.gene49046:CDS 30.0%
CTTTAGTGTTACCTCTAATA+GGG - chr1.3:69919137-69919156 MS.gene49046:CDS 30.0%
GCTAAGAAATTAACTGGAAT+TGG - chr1.3:69919415-69919434 MS.gene49046:CDS 30.0%
TAAAGCCAAAGTAACGAAAT+AGG + chr1.3:69918392-69918411 None:intergenic 30.0%
TCTTTAGTGTTACCTCTAAT+AGG - chr1.3:69919136-69919155 MS.gene49046:CDS 30.0%
!! TATGTTGGGTTTGATTTTCA+AGG - chr1.3:69919298-69919317 MS.gene49046:CDS 30.0%
AAAGATTGTGCTAAGAGATC+AGG + chr1.3:69918494-69918513 None:intergenic 35.0%
AGAGAATCAAAGATGCAACA+AGG + chr1.3:69918685-69918704 None:intergenic 35.0%
AGTTAAGAGTAGCAAGAGAA+TGG + chr1.3:69918363-69918382 None:intergenic 35.0%
CAAAATTCCCATAGAAGCAA+CGG + chr1.3:69918885-69918904 None:intergenic 35.0%
CTATCCCTATTTCGTTACTT+TGG - chr1.3:69918384-69918403 MS.gene49046:CDS 35.0%
GCAAAACTTTCAGCTATAGT+AGG - chr1.3:69919004-69919023 MS.gene49046:CDS 35.0%
GTGTAGCTATATCTTCTTCT+TGG + chr1.3:69918442-69918461 None:intergenic 35.0%
TTTAGTGTTTCCACTAGAGT+TGG - chr1.3:69918950-69918969 MS.gene49046:CDS 35.0%
! CTCTCTTGTAGCTTCATTTT+AGG - chr1.3:69919028-69919047 MS.gene49046:CDS 35.0%
!! AAGTTTGTTACCAACTCTAG+TGG + chr1.3:69918963-69918982 None:intergenic 35.0%
!! ACTAGAGTTGGTAACAAACT+TGG - chr1.3:69918962-69918981 MS.gene49046:CDS 35.0%
!! ATCAAAGGTGTAGCTTTAAG+TGG - chr1.3:69918641-69918660 MS.gene49046:CDS 35.0%
!! ATTTTCAAGGTTTGTGGCTT+GGG - chr1.3:69919311-69919330 MS.gene49046:CDS 35.0%
!! GCTGAAAGTTTTGCTTTTGA+TGG + chr1.3:69918998-69919017 None:intergenic 35.0%
!! GGTTTGATTTTCAAGGTTTG+TGG - chr1.3:69919305-69919324 MS.gene49046:CDS 35.0%
!! TCAAAGGTGTAGCTTTAAGT+GGG - chr1.3:69918642-69918661 MS.gene49046:CDS 35.0%
!! TGTTTTGAGTCAAACGGATT+GGG - chr1.3:69919375-69919394 MS.gene49046:CDS 35.0%
!!! GTTGGTTGTTTTGAGTCAAA+CGG - chr1.3:69919369-69919388 MS.gene49046:CDS 35.0%
!!! TTGTTTTGAGTCAAACGGAT+TGG - chr1.3:69919374-69919393 MS.gene49046:CDS 35.0%
AAAGCTAACCAAATTGCCAC+AGG + chr1.3:69919277-69919296 None:intergenic 40.0%
AAAGTAGCACAAAGTGTGAG+AGG + chr1.3:69918563-69918582 None:intergenic 40.0%
AACTTGTGCTGTGACTATGT+TGG - chr1.3:69919351-69919370 MS.gene49046:CDS 40.0%
AGGCAAACAGAAAGACAACT+TGG + chr1.3:69918800-69918819 None:intergenic 40.0%
AGTTCACATAGCCCTATTAG+AGG + chr1.3:69919151-69919170 None:intergenic 40.0%
ATGATCTCCATGCTCTTTCT+TGG - chr1.3:69918173-69918192 MS.gene49046:CDS 40.0%
CTAATAGGGCTATGTGAACT+TGG - chr1.3:69919151-69919170 MS.gene49046:CDS 40.0%
CTTCTTGGCCACATAAAAGA+AGG + chr1.3:69918427-69918446 None:intergenic 40.0%
GGAAATTGTCCACAAACAAC+AGG - chr1.3:69919172-69919191 MS.gene49046:CDS 40.0%
GTAGGAATGGAACCTATTTG+TGG - chr1.3:69918284-69918303 MS.gene49046:CDS 40.0%
GTCTTTCTGTTTGCCTTGAA+TGG - chr1.3:69918804-69918823 MS.gene49046:CDS 40.0%
TGGCACACACAAGAAAACAT+GGG - chr1.3:69918718-69918737 MS.gene49046:CDS 40.0%
TTACATTGTTGGAATGCCTG+TGG - chr1.3:69919258-69919277 MS.gene49046:CDS 40.0%
TTGTGCTAAGAGATCAGGAA+TGG + chr1.3:69918489-69918508 None:intergenic 40.0%
TTTCATACCACCACCATTCA+AGG + chr1.3:69918820-69918839 None:intergenic 40.0%
TTTCTGTTTGCCTTGAATGG+TGG - chr1.3:69918807-69918826 MS.gene49046:CDS 40.0%
! ACCAAGGAGAGTGAATCTTT+TGG + chr1.3:69918324-69918343 None:intergenic 40.0%
! AGTGAATCTTTTGGCACCAA+AGG + chr1.3:69918315-69918334 None:intergenic 40.0%
! CTTGTTTCTCACCTCAACAT+GGG - chr1.3:69918617-69918636 MS.gene49046:CDS 40.0%
! TCTTGTTTCTCACCTCAACA+TGG - chr1.3:69918616-69918635 MS.gene49046:CDS 40.0%
!! CAAAGGTGTAGCTTTAAGTG+GGG - chr1.3:69918643-69918662 MS.gene49046:CDS 40.0%
!! GAGTAATGGATTGGCTTCTA+AGG + chr1.3:69918538-69918557 None:intergenic 40.0%
!! GATTTTCAAGGTTTGTGGCT+TGG - chr1.3:69919310-69919329 MS.gene49046:CDS 40.0%
AAGAGCAACCGTTGCTTCTA+TGG - chr1.3:69918874-69918893 MS.gene49046:CDS 45.0%
AAGGTGACCAAGAAAGAGCA+TGG + chr1.3:69918183-69918202 None:intergenic 45.0%
AAGTGTGAGAGGCAGAGTAA+TGG + chr1.3:69918552-69918571 None:intergenic 45.0%
ACACACAAGAAAACATGGGG+TGG - chr1.3:69918722-69918741 MS.gene49046:CDS 45.0%
AGAGCAACCGTTGCTTCTAT+GGG - chr1.3:69918875-69918894 MS.gene49046:CDS 45.0%
ATAGAAGCAACGGTTGCTCT+TGG + chr1.3:69918875-69918894 None:intergenic 45.0%
ATGCTCTTTCTTGGTCACCT+TGG - chr1.3:69918182-69918201 MS.gene49046:CDS 45.0%
CACCTCAACATGGGAATCAA+AGG - chr1.3:69918626-69918645 MS.gene49046:CDS 45.0%
CACCTTTGATTCCCATGTTG+AGG + chr1.3:69918631-69918650 None:intergenic 45.0%
CCAAAGGCTTGTCCACAAAT+AGG + chr1.3:69918299-69918318 None:intergenic 45.0%
CCTATTTGTGGACAAGCCTT+TGG - chr1.3:69918296-69918315 MS.gene49046:CDS 45.0%
CTGGCACACACAAGAAAACA+TGG - chr1.3:69918717-69918736 MS.gene49046:CDS 45.0%
GCCAAAAGATTCACTCTCCT+TGG - chr1.3:69918320-69918339 MS.gene49046:CDS 45.0%
GGCACACACAAGAAAACATG+GGG - chr1.3:69918719-69918738 MS.gene49046:CDS 45.0%
GTCCACAAACAACAGGTTGT+GGG - chr1.3:69919179-69919198 MS.gene49046:CDS 45.0%
TCCACAAACAACAGGTTGTG+GGG - chr1.3:69919180-69919199 MS.gene49046:CDS 45.0%
TGTCCACAAACAACAGGTTG+TGG - chr1.3:69919178-69919197 MS.gene49046:CDS 45.0%
TTGGAATGCCTGTGGCAATT+TGG - chr1.3:69919266-69919285 MS.gene49046:CDS 45.0%
!! TGAGAGGCAGAGTAATGGAT+TGG + chr1.3:69918547-69918566 None:intergenic 45.0%
ACCGGAGAGGATTGAGTAAC+CGG + chr1.3:69918258-69918277 None:intergenic 50.0%
ACCGGTTACTCAATCCTCTC+CGG - chr1.3:69918254-69918273 MS.gene49046:CDS 50.0%
CTGTTTGCCTTGAATGGTGG+TGG - chr1.3:69918810-69918829 MS.gene49046:CDS 50.0%
CTTGGTGAACTTGCCTTAGC+CGG - chr1.3:69918200-69918219 MS.gene49046:CDS 50.0%
GGAACAGCAAGGCCTAAAGT+AGG - chr1.3:69919211-69919230 MS.gene49046:CDS 50.0%
GGGTGTTAAGAGGAACAGCA+AGG - chr1.3:69919200-69919219 MS.gene49046:CDS 50.0%
TTCCATTCCTACAGCGAGAC+CGG + chr1.3:69918276-69918295 None:intergenic 50.0%
! ACAGGTTGTGGGGTGTTAAG+AGG - chr1.3:69919190-69919209 MS.gene49046:CDS 50.0%
! ACCCCACAACCTGTTGTTTG+TGG + chr1.3:69919184-69919203 None:intergenic 50.0%
! TTTGTGGCTTGGGTTGCTTG+TGG - chr1.3:69919321-69919340 MS.gene49046:CDS 50.0%
!! GTGTAGCTTTAAGTGGGGTG+TGG - chr1.3:69918648-69918667 MS.gene49046:CDS 50.0%
CGGCTAAGGCAAGTTCACCA+AGG + chr1.3:69918202-69918221 None:intergenic 55.0%
! CTTGGGTTGCTTGTGGCTCA+GGG - chr1.3:69919328-69919347 MS.gene49046:CDS 55.0%
! GTCGGCTTCGCGAACATTAC+CGG - chr1.3:69918236-69918255 MS.gene49046:CDS 55.0%
ATCCTCTCCGGTCTCGCTGT+AGG - chr1.3:69918266-69918285 MS.gene49046:CDS 60.0%
CTCCGGTCTCGCTGTAGGAA+TGG - chr1.3:69918271-69918290 MS.gene49046:CDS 60.0%
GGTGAACTTGCCTTAGCCGG+TGG - chr1.3:69918203-69918222 MS.gene49046:CDS 60.0%
TTCCTACAGCGAGACCGGAG+AGG + chr1.3:69918271-69918290 None:intergenic 60.0%
! GCTTGGGTTGCTTGTGGCTC+AGG - chr1.3:69919327-69919346 MS.gene49046:CDS 60.0%
CTTAGCCGGTGGCTCACTCG+CGG - chr1.3:69918214-69918233 MS.gene49046:CDS 65.0%
! CGCGAGTGAGCCACCGGCTA+AGG + chr1.3:69918216-69918235 None:intergenic 70.0%
GCCGACCGCGAGTGAGCCAC+CGG + chr1.3:69918222-69918241 None:intergenic 75.0%
GCCGGTGGCTCACTCGCGGT+CGG - chr1.3:69918218-69918237 MS.gene49046:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 69918173 69919534 69918173 ID=MS.gene49046
chr1.3 mRNA 69918173 69919534 69918173 ID=MS.gene49046.t1;Parent=MS.gene49046
chr1.3 exon 69918173 69919534 69918173 ID=MS.gene49046.t1.exon1;Parent=MS.gene49046.t1
chr1.3 CDS 69918173 69919534 69918173 ID=cds.MS.gene49046.t1;Parent=MS.gene49046.t1
Gene Sequence

>MS.gene49046

ATGATCTCCATGCTCTTTCTTGGTCACCTTGGTGAACTTGCCTTAGCCGGTGGCTCACTCGCGGTCGGCTTCGCGAACATTACCGGTTACTCAATCCTCTCCGGTCTCGCTGTAGGAATGGAACCTATTTGTGGACAAGCCTTTGGTGCCAAAAGATTCACTCTCCTTGGTCTATGTTTACAAAAAACCATTCTCTTGCTACTCTTAACTTCTATCCCTATTTCGTTACTTTGGCTTTACACAAAACATATCCTTCTTTTATGTGGCCAAGAAGAAGATATAGCTACACAAGCTCAAATCTATCTTCTATATTCCATTCCTGATCTCTTAGCACAATCTTTTTTACACCCTTTAAGAATTTACCTTAGAAGCCAATCCATTACTCTGCCTCTCACACTTTGTGCTACTTTAGCAATTTTTCTTCACATTCCCATAAACTATTTTCTTGTTTCTCACCTCAACATGGGAATCAAAGGTGTAGCTTTAAGTGGGGTGTGGACAAATTTCAACCTTGTTGCATCTTTGATTCTCTACATAATTTTCTCTGGCACACACAAGAAAACATGGGGTGGTTTTTCATCAGAATGTTTCAAACAATGGAAATCACTTCTTAATTTAGCTGTACCAAGTTGTCTTTCTGTTTGCCTTGAATGGTGGTGGTATGAAATCATGATTTTGTTATGTGGTTTGTTGATAAATCCAAGAGCAACCGTTGCTTCTATGGGAATTTTGATTCAAACTACTTCTTTGCTATATATTTTTCCATCATCAATAAGTTTTAGTGTTTCCACTAGAGTTGGTAACAAACTTGGTGCTCAAAAGCCATCAAAAGCAAAACTTTCAGCTATAGTAGGACTCTCTTGTAGCTTCATTTTAGGTGTGTTTGCTTTGTTTTTTGCTATAATGGTTAGGAACATTTGGGCTAGCATGTTCACTGAAGACAAAGAGATAATAAAAATTACATCTTTAGTGTTACCTCTAATAGGGCTATGTGAACTTGGAAATTGTCCACAAACAACAGGTTGTGGGGTGTTAAGAGGAACAGCAAGGCCTAAAGTAGGTGCAAATATTAATTTTGGTTGTTTTTACATTGTTGGAATGCCTGTGGCAATTTGGTTAGCTTTTTATGTTGGGTTTGATTTTCAAGGTTTGTGGCTTGGGTTGCTTGTGGCTCAGGGAACTTGTGCTGTGACTATGTTGGTTGTTTTGAGTCAAACGGATTGGGATTGTGAAGCACTAAGAGCTAAGAAATTAACTGGAATTGGAGAAGCAACAACAAGAAGTGATGTTTTTGTTGATGACAGCAAAGAAGTTGATGAAGAGAAGTTACTTAAAGCAGAAATTAAGGAATATTCTTCTTAA
Protein sequence

>MS.gene49046.t1

MISMLFLGHLGELALAGGSLAVGFANITGYSILSGLAVGMEPICGQAFGAKRFTLLGLCLQKTILLLLLTSIPISLLWLYTKHILLLCGQEEDIATQAQIYLLYSIPDLLAQSFLHPLRIYLRSQSITLPLTLCATLAIFLHIPINYFLVSHLNMGIKGVALSGVWTNFNLVASLILYIIFSGTHKKTWGGFSSECFKQWKSLLNLAVPSCLSVCLEWWWYEIMILLCGLLINPRATVASMGILIQTTSLLYIFPSSISFSVSTRVGNKLGAQKPSKAKLSAIVGLSCSFILGVFALFFAIMVRNIWASMFTEDKEIIKITSLVLPLIGLCELGNCPQTTGCGVLRGTARPKVGANINFGCFYIVGMPVAIWLAFYVGFDFQGLWLGLLVAQGTCAVTMLVVLSQTDWDCEALRAKKLTGIGEATTRSDVFVDDSKEVDEEKLLKAEIKEYSS