AlfalfaGEDB | MS.gene002950

M. sativa cultivar XinJiangDaYe / MS.gene002950

KEGG: K02115
GO: GO:0003674 GO:0003824 GO:0005215 GO:0005216 GO:0005261 GO:0005575 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009165 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0015075 GO:0015077 GO:0015078 GO:0015252 GO:0015267 GO:0015318 GO:0015672 GO:0015985 GO:0015986 GO:0016740 GO:0016772 GO:0016776 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0022803 GO:0022857 GO:0022890 GO:0032991 GO:0033178 GO:0034220 GO:0034641 GO:0034654 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0045261 GO:0046034 GO:0046390 GO:0046483 GO:0046933 GO:0051179 GO:0051234 GO:0055085 GO:0055086 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
PFAM: PF00231.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002950.t1	ACJ84643.1	93.3	374	25	0	1	374	1	374	1.50E-186	662.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002950.t1	P28552	91.9	369	30	0	1	369	1	369	4.2e-174	612.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002950.t1	B7FIR4	93.3	374	25	0	1	374	1	374	1.1e-186	662.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene012472	MS.gene002950	PPI
MS.gene055477	MS.gene002950	PPI
MS.gene035640	MS.gene002950	PPI
MS.gene57960	MS.gene002950	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002950.t1	MTR_2g023290	98.294	293	5	0	82	374	729	1021	0.0	590
MS.gene002950.t1	MTR_3g052270	68.155	336	101	2	44	373	12	347	1.68e-168	474
MS.gene002950.t1	MTR_1588s0010	99.010	101	1	0	1	101	1	101	2.65e-65	202
MS.gene002950.t1	MTR_4g129790	29.380	371	194	12	9	373	17	325	9.02e-27	108

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002950.t1	AT4G04640	81.383	376	65	3	1	374	1	373	0.0	603
MS.gene002950.t1	AT1G15700	70.725	345	100	1	30	373	39	383	2.46e-177	498
MS.gene002950.t1	AT2G33040	31.661	319	171	10	57	373	48	321	4.81e-28	112

Find 58 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTATCAGTGTTGGTAAAA+AGG	0.215367	2.4:-64015647	MS.gene002950:CDS
GCTCAAGAAGCTGTTGTTAA+TGG	0.249925	2.4:-64015892	MS.gene002950:CDS
GGTGAAATATGTGATATTAA+TGG	0.261231	2.4:-64015406	MS.gene002950:CDS
TCAAATCTTGAGGGCTTTGC+AGG	0.276662	2.4:-64015209	MS.gene002950:CDS
ATATCACATATTTCACCTTT+TGG	0.288857	2.4:+64015412	None:intergenic
GTGACAGCGGAAGCAAAGTA+TGG	0.341651	2.4:+64015434	None:intergenic
GGAAGCAAAGTATGGATTAT+AGG	0.360427	2.4:+64015442	None:intergenic
AACCTATCAACAGGTATATA+AGG	0.362640	2.4:+64015601	None:intergenic
TCCTGCTCAAACTGCAAAAT+CGG	0.366440	2.4:+64015277	None:intergenic
TGAGACCAACATGGTGATAT+TGG	0.370949	2.4:+64016134	None:intergenic
ACTGGTCTAAGCTTAGTTAG+AGG	0.375333	2.4:+64015793	None:intergenic
CAGCACCAGCAACAATTTCC+AGG	0.387882	2.4:+64015056	None:intergenic
ATTGCTGAGTTGAAAGGTCT+TGG	0.399341	2.4:-64015682	MS.gene002950:CDS
GACCTTATATACCTGTTGAT+AGG	0.399954	2.4:-64015603	MS.gene002950:CDS
TGAATTTGAGTTGGTCTTGA+AGG	0.403080	2.4:+64016015	None:intergenic
CTGTTGAAAGAGATGCTGTT+AGG	0.407775	2.4:-64015321	MS.gene002950:CDS
TGCATCAGGTAATGAAGGTT+TGG	0.412967	2.4:+64016110	None:intergenic
CCTGTTGATAGGTTTCTTGA+AGG	0.420186	2.4:-64015592	MS.gene002950:CDS
TTCAGGTTAACAACGAAAGA+AGG	0.449455	2.4:-64015352	MS.gene002950:CDS
TTTGAACAGTCAAATCTTGA+GGG	0.462626	2.4:-64015218	MS.gene002950:CDS
GTTGCTCTTGTTGTTTGCAC+TGG	0.463249	2.4:-64015763	MS.gene002950:CDS
TGTTGCTGGTGCTGATGCCT+TGG	0.464852	2.4:-64015047	MS.gene002950:CDS
CCTTCAAGAAACCTATCAAC+AGG	0.466272	2.4:+64015592	None:intergenic
TCAACTAGTGTCTGTGAGAA+AGG	0.467803	2.4:+64015865	None:intergenic
ATTTGACTGTTCAAATAAAG+TGG	0.475054	2.4:+64015226	None:intergenic
TAAGATCACTGGTGAAATCC+TGG	0.483893	2.4:-64015074	MS.gene002950:CDS
AGTGCATCAAGAATCTGAAC+AGG	0.485687	2.4:+64015253	None:intergenic
GAAATCCTGGAAATTGTTGC+TGG	0.495138	2.4:-64015061	MS.gene002950:CDS
AAGGAGAGGTTAGATGCATC+AGG	0.495737	2.4:+64016096	None:intergenic
GTTGTTTGCACTGGTGATAG+AGG	0.498449	2.4:-64015754	MS.gene002950:CDS
GTGCTTCTTTAGCAGTAGGA+AGG	0.507770	2.4:+64015563	None:intergenic
TTGCTTCCGCTGTCACCAAA+AGG	0.508071	2.4:-64015427	MS.gene002950:CDS
TTAGATGCATCAGGTAATGA+AGG	0.514624	2.4:+64016105	None:intergenic
GTTGATAGGTTTCTTGAAGG+TGG	0.518256	2.4:-64015589	MS.gene002950:CDS
TTTCACCAGTGATCTTAGCT+TGG	0.530031	2.4:+64015080	None:intergenic
AGACCACACTGAATTTGAGT+TGG	0.535135	2.4:+64016006	None:intergenic
AATAGGATTGCTGAGTTGAA+AGG	0.535194	2.4:-64015688	MS.gene002950:CDS
GTTTGTGCTTCTTTAGCAGT+AGG	0.537662	2.4:+64015559	None:intergenic
ATTTGAACAGTCAAATCTTG+AGG	0.540431	2.4:-64015219	MS.gene002950:CDS
TTTGTGAAGGAAGCTGAAAA+GGG	0.543827	2.4:+64016058	None:intergenic
GATTACACTATTATCAGTGT+TGG	0.547808	2.4:-64015655	MS.gene002950:CDS
GCTGTTCGTTGATGCTGTAA+AGG	0.551485	2.4:+64015839	None:intergenic
GTTGAAGAAGAATTTGTCAA+GGG	0.556839	2.4:-64015119	MS.gene002950:CDS
TTGCTCCAATATCACCATGT+TGG	0.572873	2.4:-64016139	MS.gene002950:CDS
AGTTGAAGAAGAATTTGTCA+AGG	0.578085	2.4:-64015120	MS.gene002950:CDS
CAACGCCAAGCTAAGATCAC+TGG	0.583862	2.4:-64015085	MS.gene002950:CDS
AGGGCTTAGAGCTGAACGGA+AGG	0.590344	2.4:+64016077	None:intergenic
TAGAGGAGCTTCGATATCGT+CGG	0.592502	2.4:+64015810	None:intergenic
GGTGATAGAGGTCTCTGTGG+TGG	0.597310	2.4:-64015742	MS.gene002950:CDS
CACTGAAGCAATGAAGCTTG+TGG	0.611051	2.4:-64015935	MS.gene002950:CDS
AAACTGTGTTGATGCTGCTG+AGG	0.639433	2.4:-64015383	MS.gene002950:CDS
TTAGAGCTGAACGGAAGGAG+AGG	0.640227	2.4:+64016082	None:intergenic
CATAAATACACTCTATACCA+AGG	0.645423	2.4:+64015030	None:intergenic
GAAAAGGGCTTAGAGCTGAA+CGG	0.645819	2.4:+64016073	None:intergenic
CAGGTATATAAGGTCTACGA+AGG	0.649710	2.4:+64015611	None:intergenic
ACTGGTGATAGAGGTCTCTG+TGG	0.722605	2.4:-64015745	MS.gene002950:CDS
AGACCAACTCAAATTCAGTG+TGG	0.750415	2.4:-64016009	MS.gene002950:CDS
AGGTTTGGATGAGACCAACA+TGG	0.753902	2.4:+64016125	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTGAAAAAAGCTGAAAAT+AGG	-	chr2.4:64015481-64015500	MS.gene002950:CDS	20.0%
!	ATTTGACTGTTCAAATAAAG+TGG	+	chr2.4:64015963-64015982	None:intergenic	25.0%
!	GGTGAAATATGTGATATTAA+TGG	-	chr2.4:64015780-64015799	MS.gene002950:CDS	25.0%
!!	ATATCACATATTTCACCTTT+TGG	+	chr2.4:64015777-64015796	None:intergenic	25.0%
!!!	ATTATCAGTGTTGGTAAAAA+GGG	-	chr2.4:64015540-64015559	MS.gene002950:CDS	25.0%
!!!	TATTATCAGTGTTGGTAAAA+AGG	-	chr2.4:64015539-64015558	MS.gene002950:CDS	25.0%
	AACCTATCAACAGGTATATA+AGG	+	chr2.4:64015588-64015607	None:intergenic	30.0%
	AGTTGAAGAAGAATTTGTCA+AGG	-	chr2.4:64016066-64016085	MS.gene002950:CDS	30.0%
	ATTTGAACAGTCAAATCTTG+AGG	-	chr2.4:64015967-64015986	MS.gene002950:CDS	30.0%
	GATTACACTATTATCAGTGT+TGG	-	chr2.4:64015531-64015550	MS.gene002950:CDS	30.0%
	GTTGAAGAAGAATTTGTCAA+GGG	-	chr2.4:64016067-64016086	MS.gene002950:CDS	30.0%
	TTTGAACAGTCAAATCTTGA+GGG	-	chr2.4:64015968-64015987	MS.gene002950:CDS	30.0%
!!	TGTTTTTGACAGAGTCAATA+CGG	+	chr2.4:64015221-64015240	None:intergenic	30.0%
	GACCTTATATACCTGTTGAT+AGG	-	chr2.4:64015583-64015602	MS.gene002950:CDS	35.0%
	TTAGATGCATCAGGTAATGA+AGG	+	chr2.4:64015084-64015103	None:intergenic	35.0%
	TTTGTGAAGGAAGCTGAAAA+GGG	+	chr2.4:64015131-64015150	None:intergenic	35.0%
!	AATAGGATTGCTGAGTTGAA+AGG	-	chr2.4:64015498-64015517	MS.gene002950:CDS	35.0%
!	GGAAGCAAAGTATGGATTAT+AGG	+	chr2.4:64015747-64015766	None:intergenic	35.0%
!	TTCAGGTTAACAACGAAAGA+AGG	-	chr2.4:64015834-64015853	MS.gene002950:CDS	35.0%
!	TTTTGTGAAGGAAGCTGAAA+AGG	+	chr2.4:64015132-64015151	None:intergenic	35.0%
!!	GAAGATGATGAGTTTTGTGA+AGG	+	chr2.4:64015144-64015163	None:intergenic	35.0%
!!	TGAATTTGAGTTGGTCTTGA+AGG	+	chr2.4:64015174-64015193	None:intergenic	35.0%
!!!	ACAAGAGCAACTTTTTTCAC+TGG	+	chr2.4:64015414-64015433	None:intergenic	35.0%
	AGACCAACTCAAATTCAGTG+TGG	-	chr2.4:64015177-64015196	MS.gene002950:CDS	40.0%
	AGACCACACTGAATTTGAGT+TGG	+	chr2.4:64015183-64015202	None:intergenic	40.0%
	AGTGCATCAAGAATCTGAAC+AGG	+	chr2.4:64015936-64015955	None:intergenic	40.0%
	CAGGTATATAAGGTCTACGA+AGG	+	chr2.4:64015578-64015597	None:intergenic	40.0%
	CCTTCAAGAAACCTATCAAC+AGG	+	chr2.4:64015597-64015616	None:intergenic	40.0%
	CTGTTGAAAGAGATGCTGTT+AGG	-	chr2.4:64015865-64015884	MS.gene002950:CDS	40.0%
	GAAATCCTGGAAATTGTTGC+TGG	-	chr2.4:64016125-64016144	MS.gene002950:CDS	40.0%
	GCTCAAGAAGCTGTTGTTAA+TGG	-	chr2.4:64015294-64015313	MS.gene002950:CDS	40.0%
	GTTTGTGCTTCTTTAGCAGT+AGG	+	chr2.4:64015630-64015649	None:intergenic	40.0%
	TAAGATCACTGGTGAAATCC+TGG	-	chr2.4:64016112-64016131	MS.gene002950:CDS	40.0%
	TCAACTAGTGTCTGTGAGAA+AGG	+	chr2.4:64015324-64015343	None:intergenic	40.0%
	TCCTGCTCAAACTGCAAAAT+CGG	+	chr2.4:64015912-64015931	None:intergenic	40.0%
	TGAGACCAACATGGTGATAT+TGG	+	chr2.4:64015055-64015074	None:intergenic	40.0%
	TGCATCAGGTAATGAAGGTT+TGG	+	chr2.4:64015079-64015098	None:intergenic	40.0%
	TTGCTCCAATATCACCATGT+TGG	-	chr2.4:64015047-64015066	MS.gene002950:CDS	40.0%
	TTTCACCAGTGATCTTAGCT+TGG	+	chr2.4:64016109-64016128	None:intergenic	40.0%
!	ACTGGTCTAAGCTTAGTTAG+AGG	+	chr2.4:64015396-64015415	None:intergenic	40.0%
!	ATTGCTGAGTTGAAAGGTCT+TGG	-	chr2.4:64015504-64015523	MS.gene002950:CDS	40.0%
!	CCTGTTGATAGGTTTCTTGA+AGG	-	chr2.4:64015594-64015613	MS.gene002950:CDS	40.0%
!	GTTGATAGGTTTCTTGAAGG+TGG	-	chr2.4:64015597-64015616	MS.gene002950:CDS	40.0%
!!	ATTTCACCTTTTGGTGACAG+CGG	+	chr2.4:64015768-64015787	None:intergenic	40.0%
	AAGGAGAGGTTAGATGCATC+AGG	+	chr2.4:64015093-64015112	None:intergenic	45.0%
	AGGTTTGGATGAGACCAACA+TGG	+	chr2.4:64015064-64015083	None:intergenic	45.0%
	CACTGAAGCAATGAAGCTTG+TGG	-	chr2.4:64015251-64015270	MS.gene002950:CDS	45.0%
	GAAAAGGGCTTAGAGCTGAA+CGG	+	chr2.4:64015116-64015135	None:intergenic	45.0%
	GCTGTTCGTTGATGCTGTAA+AGG	+	chr2.4:64015350-64015369	None:intergenic	45.0%
	GTGCTTCTTTAGCAGTAGGA+AGG	+	chr2.4:64015626-64015645	None:intergenic	45.0%
	TAGAGGAGCTTCGATATCGT+CGG	+	chr2.4:64015379-64015398	None:intergenic	45.0%
	TCAAATCTTGAGGGCTTTGC+AGG	-	chr2.4:64015977-64015996	MS.gene002950:CDS	45.0%
!	AAACTGTGTTGATGCTGCTG+AGG	-	chr2.4:64015803-64015822	MS.gene002950:CDS	45.0%
!	GTTGTTTGCACTGGTGATAG+AGG	-	chr2.4:64015432-64015451	MS.gene002950:CDS	45.0%
!!	GTTGCTCTTGTTGTTTGCAC+TGG	-	chr2.4:64015423-64015442	MS.gene002950:CDS	45.0%
	ACTGGTGATAGAGGTCTCTG+TGG	-	chr2.4:64015441-64015460	MS.gene002950:CDS	50.0%
	CAACGCCAAGCTAAGATCAC+TGG	-	chr2.4:64016101-64016120	MS.gene002950:CDS	50.0%
	CAGCACCAGCAACAATTTCC+AGG	+	chr2.4:64016133-64016152	None:intergenic	50.0%
	GTGACAGCGGAAGCAAAGTA+TGG	+	chr2.4:64015755-64015774	None:intergenic	50.0%
	TTAGAGCTGAACGGAAGGAG+AGG	+	chr2.4:64015107-64015126	None:intergenic	50.0%
	TTGCTTCCGCTGTCACCAAA+AGG	-	chr2.4:64015759-64015778	MS.gene002950:CDS	50.0%
!	CTGCTGAGGATGAGCTTTTC+AGG	-	chr2.4:64015817-64015836	MS.gene002950:CDS	50.0%
!!	GCCGATTTTGCAGTTTGAGC+AGG	-	chr2.4:64015908-64015927	MS.gene002950:CDS	50.0%
	AGGGCTTAGAGCTGAACGGA+AGG	+	chr2.4:64015112-64015131	None:intergenic	55.0%
	GGTGATAGAGGTCTCTGTGG+TGG	-	chr2.4:64015444-64015463	MS.gene002950:CDS	55.0%
!!	TGTTGCTGGTGCTGATGCCT+TGG	-	chr2.4:64016139-64016158	MS.gene002950:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	64015042	64016166	64015042	ID=MS.gene002950
chr2.4	mRNA	64015042	64016166	64015042	ID=MS.gene002950.t1;Parent=MS.gene002950
chr2.4	exon	64015042	64016166	64015042	ID=MS.gene002950.t1.exon1;Parent=MS.gene002950.t1
chr2.4	CDS	64015042	64016166	64015042	ID=cds.MS.gene002950.t1;Parent=MS.gene002950.t1

Gene Sequence

>MS.gene002950

ATGTCTTGCTCCAATATCACCATGTTGGTCTCATCCAAACCTTCATTACCTGATGCATCTAACCTCTCCTTCCGTTCAGCTCTAAGCCCTTTTCAGCTTCCTTCACAAAACTCATCATCTTCTTGCACTCCTTCAAGACCAACTCAAATTCAGTGTGGTCTAAGAGAACTCAGAAACCGTATTGACTCTGTCAAAAACACTCAGAAAATCACTGAAGCAATGAAGCTTGTGGCTGCTGCAAAAGTCAGAAGAGCTCAAGAAGCTGTTGTTAATGGCAGACCTTTCTCACAGACACTAGTTGAAGTCCTTTACAGCATCAACGAACAGCTTCAAACCGACGATATCGAAGCTCCTCTAACTAAGCTTAGACCAGTGAAAAAAGTTGCTCTTGTTGTTTGCACTGGTGATAGAGGTCTCTGTGGTGGATTCAACAATGCAATATTGAAAAAAGCTGAAAATAGGATTGCTGAGTTGAAAGGTCTTGGACTTGATTACACTATTATCAGTGTTGGTAAAAAGGGTAACTCTTATTTCCTTCGTAGACCTTATATACCTGTTGATAGGTTTCTTGAAGGTGGAAACCTTCCTACTGCTAAAGAAGCACAAACTATTGCTGATGATGTTTTCTCACTCTTTGTTAGTGAAGAAGTTGATAAAGTTGAACTTTTGTACACTAAATTTGTGTCATTAGTGAAATCAGAGCCTATAATCCATACTTTGCTTCCGCTGTCACCAAAAGGTGAAATATGTGATATTAATGGAAACTGTGTTGATGCTGCTGAGGATGAGCTTTTCAGGTTAACAACGAAAGAAGGAAAGTTAACTGTTGAAAGAGATGCTGTTAGGTCTAAGACGATTGATTTTTCGCCGATTTTGCAGTTTGAGCAGGATCCTGTTCAGATTCTTGATGCACTTTTGCCACTTTATTTGAACAGTCAAATCTTGAGGGCTTTGCAGGAATCACTTGCTAGTGAATTAGCTGCTAGAATGAGTGCTATGAGTAGTGCTTGTGATAATGCTATTGAGTTGAAGAAGAATTTGTCAAGGGTTTATAATAGACAACGCCAAGCTAAGATCACTGGTGAAATCCTGGAAATTGTTGCTGGTGCTGATGCCTTGGTATAG

Protein sequence

>MS.gene002950.t1

MSCSNITMLVSSKPSLPDASNLSFRSALSPFQLPSQNSSSSCTPSRPTQIQCGLRELRNRIDSVKNTQKITEAMKLVAAAKVRRAQEAVVNGRPFSQTLVEVLYSINEQLQTDDIEAPLTKLRPVKKVALVVCTGDRGLCGGFNNAILKKAENRIAELKGLGLDYTIISVGKKGNSYFLRRPYIPVDRFLEGGNLPTAKEAQTIADDVFSLFVSEEVDKVELLYTKFVSLVKSEPIIHTLLPLSPKGEICDINGNCVDAAEDELFRLTTKEGKLTVERDAVRSKTIDFSPILQFEQDPVQILDALLPLYLNSQILRALQESLASELAARMSAMSSACDNAIELKKNLSRVYNRQRQAKITGEILEIVAGADALV