Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003386.t1 | RHN61630.1 | 97.8 | 135 | 3 | 0 | 1 | 135 | 10 | 144 | 5.30E-70 | 273.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003386.t1 | Q0V7Z5 | 46.7 | 135 | 67 | 2 | 1 | 133 | 10 | 141 | 6.9e-26 | 118.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003386.t1 | A0A072ULN9 | 97.8 | 135 | 3 | 0 | 1 | 135 | 1 | 135 | 3.9e-70 | 273.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene003386 | MS.gene28935 | PPI |
| MS.gene052293 | MS.gene003386 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003386.t1 | MTR_4g075760 | 97.778 | 135 | 3 | 0 | 1 | 135 | 1 | 135 | 1.31e-97 | 276 |
| MS.gene003386.t1 | MTR_4g072910 | 39.326 | 89 | 47 | 4 | 9 | 94 | 14 | 98 | 3.90e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene003386.t1 | AT1G29450 | 46.667 | 135 | 67 | 2 | 1 | 133 | 10 | 141 | 3.92e-35 | 119 |
| MS.gene003386.t1 | AT1G29450 | 46.667 | 135 | 67 | 2 | 1 | 133 | 10 | 141 | 3.92e-35 | 119 |
| MS.gene003386.t1 | AT1G29420 | 53.279 | 122 | 54 | 2 | 1 | 119 | 10 | 131 | 6.81e-34 | 115 |
| MS.gene003386.t1 | AT1G29430 | 53.922 | 102 | 46 | 1 | 5 | 105 | 15 | 116 | 1.94e-33 | 114 |
| MS.gene003386.t1 | AT1G29420 | 53.279 | 122 | 54 | 2 | 1 | 119 | 50 | 171 | 4.04e-33 | 115 |
| MS.gene003386.t1 | AT1G29510 | 45.736 | 129 | 68 | 2 | 5 | 132 | 15 | 142 | 2.28e-32 | 112 |
| MS.gene003386.t1 | AT5G27780 | 55.140 | 107 | 46 | 1 | 1 | 105 | 11 | 117 | 3.73e-32 | 111 |
| MS.gene003386.t1 | AT1G29500 | 51.429 | 105 | 49 | 1 | 1 | 105 | 10 | 112 | 8.89e-32 | 110 |
| MS.gene003386.t1 | AT1G29460 | 49.558 | 113 | 49 | 1 | 1 | 105 | 10 | 122 | 8.61e-31 | 108 |
| MS.gene003386.t1 | AT1G29460 | 49.558 | 113 | 49 | 1 | 1 | 105 | 48 | 160 | 9.03e-31 | 108 |
| MS.gene003386.t1 | AT1G29440 | 53.922 | 102 | 46 | 1 | 5 | 105 | 14 | 115 | 2.95e-28 | 101 |
| MS.gene003386.t1 | AT1G29490 | 52.747 | 91 | 42 | 1 | 1 | 90 | 10 | 100 | 1.62e-24 | 90.5 |
| MS.gene003386.t1 | AT1G76190 | 41.333 | 75 | 41 | 1 | 32 | 103 | 23 | 97 | 3.86e-15 | 67.4 |
| MS.gene003386.t1 | AT1G20470 | 39.394 | 66 | 40 | 0 | 30 | 95 | 23 | 88 | 8.21e-13 | 61.6 |
| MS.gene003386.t1 | AT5G66260 | 39.189 | 74 | 44 | 1 | 20 | 92 | 24 | 97 | 2.36e-11 | 57.0 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTCTTCATCAGTAAATA+AGG | 0.216090 | 4.1:+28282166 | MS.gene003386:CDS |
| TTCATATCCACTTCGTTTCT+TGG | 0.266340 | 4.1:-28282146 | None:intergenic |
| TTAATCTCTGTCATGATCTT+AGG | 0.329706 | 4.1:+28282441 | MS.gene003386:CDS |
| AAGATCATGACAGAGATTAA+AGG | 0.359430 | 4.1:-28282438 | None:intergenic |
| TGTTTAGAGAACTCTTTAAA+TGG | 0.360343 | 4.1:+28282258 | MS.gene003386:CDS |
| AATGAACAAATAATTATCTA+TGG | 0.367557 | 4.1:+28282470 | MS.gene003386:CDS |
| CTATCACACGGCAACATAAT+AGG | 0.373785 | 4.1:-28282314 | None:intergenic |
| TCATATCCACTTCGTTTCTT+GGG | 0.376786 | 4.1:-28282145 | None:intergenic |
| CAAAGCCTTCTCCACGTCTT+CGG | 0.381612 | 4.1:-28282381 | None:intergenic |
| CATTGATCGCAAGCGATTTG+TGG | 0.397346 | 4.1:+28282208 | MS.gene003386:CDS |
| AAAGCCTTCTCCACGTCTTC+GGG | 0.423323 | 4.1:-28282380 | None:intergenic |
| AACTCTTCATCAGTAAATAA+GGG | 0.445037 | 4.1:+28282167 | MS.gene003386:CDS |
| TTCGTGCTAAGATACTTTAG+AGG | 0.450847 | 4.1:-28282233 | None:intergenic |
| AAATGGTCCGAGGAAGTGTT+CGG | 0.456406 | 4.1:+28282275 | MS.gene003386:CDS |
| GAGGAAGTGTTCGGATTACC+AGG | 0.460604 | 4.1:+28282284 | MS.gene003386:CDS |
| TGGCGGTCGAAAAGCGCAAG+AGG | 0.546936 | 4.1:+28282114 | MS.gene003386:CDS |
| TGGTAATCCGAACACTTCCT+CGG | 0.549919 | 4.1:-28282282 | None:intergenic |
| GATTCCCGAAGACGTGGAGA+AGG | 0.576460 | 4.1:+28282376 | MS.gene003386:CDS |
| GTGTTCGGATTACCAGGGAA+TGG | 0.590996 | 4.1:+28282290 | MS.gene003386:CDS |
| AACATAATAGGTCCATTCCC+TGG | 0.597389 | 4.1:-28282302 | None:intergenic |
| AGAACAGATTCCCGAAGACG+TGG | 0.599588 | 4.1:+28282370 | MS.gene003386:CDS |
| CTCATACCCAAGAAACGAAG+TGG | 0.601207 | 4.1:+28282139 | MS.gene003386:CDS |
| AGAACTCTTTAAATGGTCCG+AGG | 0.631606 | 4.1:+28282265 | MS.gene003386:CDS |
| CATGTATGCTTGTCACAACG+AGG | 0.660738 | 4.1:+28282412 | MS.gene003386:CDS |
| TCCAAGAAAACACTATCACA+CGG | 0.685946 | 4.1:-28282326 | None:intergenic |
| AGGAAGTGTTCGGATTACCA+GGG | 0.722902 | 4.1:+28282285 | MS.gene003386:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAACAAATAATTATCTA+TGG | + | chr4.1:28282470-28282489 | MS.gene003386:CDS | 15.0% |
| ! | AACTCTTCATCAGTAAATAA+GGG | + | chr4.1:28282167-28282186 | MS.gene003386:CDS | 25.0% |
| ! | TGTTTAGAGAACTCTTTAAA+TGG | + | chr4.1:28282258-28282277 | MS.gene003386:CDS | 25.0% |
| AAGATCATGACAGAGATTAA+AGG | - | chr4.1:28282441-28282460 | None:intergenic | 30.0% | |
| GAACTCTTCATCAGTAAATA+AGG | + | chr4.1:28282166-28282185 | MS.gene003386:CDS | 30.0% | |
| TTAATCTCTGTCATGATCTT+AGG | + | chr4.1:28282441-28282460 | MS.gene003386:CDS | 30.0% | |
| TCATATCCACTTCGTTTCTT+GGG | - | chr4.1:28282148-28282167 | None:intergenic | 35.0% | |
| TCCAAGAAAACACTATCACA+CGG | - | chr4.1:28282329-28282348 | None:intergenic | 35.0% | |
| TTCATATCCACTTCGTTTCT+TGG | - | chr4.1:28282149-28282168 | None:intergenic | 35.0% | |
| TTCGTGCTAAGATACTTTAG+AGG | - | chr4.1:28282236-28282255 | None:intergenic | 35.0% | |
| AACATAATAGGTCCATTCCC+TGG | - | chr4.1:28282305-28282324 | None:intergenic | 40.0% | |
| AGAACTCTTTAAATGGTCCG+AGG | + | chr4.1:28282265-28282284 | MS.gene003386:CDS | 40.0% | |
| CTATCACACGGCAACATAAT+AGG | - | chr4.1:28282317-28282336 | None:intergenic | 40.0% | |
| GAAAAAGTGGCAGAAAATGG+CGG | + | chr4.1:28282097-28282116 | MS.gene003386:CDS | 40.0% | |
| CATGTATGCTTGTCACAACG+AGG | + | chr4.1:28282412-28282431 | MS.gene003386:CDS | 45.0% | |
| CATTGATCGCAAGCGATTTG+TGG | + | chr4.1:28282208-28282227 | MS.gene003386:CDS | 45.0% | |
| CTCATACCCAAGAAACGAAG+TGG | + | chr4.1:28282139-28282158 | MS.gene003386:CDS | 45.0% | |
| GGCGAAAAAGTGGCAGAAAA+TGG | + | chr4.1:28282094-28282113 | MS.gene003386:CDS | 45.0% | |
| TGGTAATCCGAACACTTCCT+CGG | - | chr4.1:28282285-28282304 | None:intergenic | 45.0% | |
| !! | AAATGGTCCGAGGAAGTGTT+CGG | + | chr4.1:28282275-28282294 | MS.gene003386:CDS | 45.0% |
| !! | AGGAAGTGTTCGGATTACCA+GGG | + | chr4.1:28282285-28282304 | MS.gene003386:CDS | 45.0% |
| !! | GCCGTGTGATAGTGTTTTCT+TGG | + | chr4.1:28282325-28282344 | MS.gene003386:CDS | 45.0% |
| AAAGCCTTCTCCACGTCTTC+GGG | - | chr4.1:28282383-28282402 | None:intergenic | 50.0% | |
| AGAACAGATTCCCGAAGACG+TGG | + | chr4.1:28282370-28282389 | MS.gene003386:CDS | 50.0% | |
| CAAAGCCTTCTCCACGTCTT+CGG | - | chr4.1:28282384-28282403 | None:intergenic | 50.0% | |
| GTGTTCGGATTACCAGGGAA+TGG | + | chr4.1:28282290-28282309 | MS.gene003386:CDS | 50.0% | |
| !! | GAGGAAGTGTTCGGATTACC+AGG | + | chr4.1:28282284-28282303 | MS.gene003386:CDS | 50.0% |
| GATTCCCGAAGACGTGGAGA+AGG | + | chr4.1:28282376-28282395 | MS.gene003386:CDS | 55.0% | |
| ! | TGGCGGTCGAAAAGCGCAAG+AGG | + | chr4.1:28282114-28282133 | MS.gene003386:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 28282092 | 28282499 | 28282092 | ID=MS.gene003386 |
| chr4.1 | mRNA | 28282092 | 28282499 | 28282092 | ID=MS.gene003386.t1;Parent=MS.gene003386 |
| chr4.1 | exon | 28282092 | 28282499 | 28282092 | ID=MS.gene003386.t1.exon1;Parent=MS.gene003386.t1 |
| chr4.1 | CDS | 28282092 | 28282499 | 28282092 | ID=cds.MS.gene003386.t1;Parent=MS.gene003386.t1 |
| Gene Sequence |
| Protein sequence |
