Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28935.t1 | RHN61630.1 | 98.5 | 135 | 2 | 0 | 1 | 135 | 10 | 144 | 2.20E-71 | 278.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28935.t1 | F4I1H5 | 47.8 | 134 | 67 | 2 | 1 | 133 | 10 | 141 | 1.4e-26 | 120.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28935.t1 | A0A072ULN9 | 98.5 | 135 | 2 | 0 | 1 | 135 | 1 | 135 | 1.6e-71 | 278.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050767 | MS.gene28935 | 0.806944 | 6.43E-50 | -1.69E-46 |
| MS.gene050770 | MS.gene28935 | 0.808843 | 2.54E-50 | -1.69E-46 |
| MS.gene052290 | MS.gene28935 | 0.821853 | 3.24E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene28935 | 0.938947 | 2.89E-99 | -1.69E-46 |
| MS.gene054376 | MS.gene28935 | 0.806531 | 7.86E-50 | -1.69E-46 |
| MS.gene055254 | MS.gene28935 | 0.816822 | 4.54E-52 | -1.69E-46 |
| MS.gene055397 | MS.gene28935 | 0.813002 | 3.19E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene052293 | MS.gene28935 | PPI |
| MS.gene003386 | MS.gene28935 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28935.t1 | MTR_4g075760 | 98.519 | 135 | 2 | 0 | 1 | 135 | 1 | 135 | 4.98e-99 | 280 |
| MS.gene28935.t1 | MTR_4g072910 | 40.230 | 87 | 45 | 4 | 9 | 92 | 14 | 96 | 4.39e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28935.t1 | AT1G29450 | 50.833 | 120 | 57 | 1 | 1 | 118 | 10 | 129 | 3.35e-34 | 116 |
| MS.gene28935.t1 | AT1G29450 | 50.833 | 120 | 57 | 1 | 1 | 118 | 10 | 129 | 3.35e-34 | 116 |
| MS.gene28935.t1 | AT1G29430 | 50.000 | 116 | 55 | 2 | 5 | 119 | 15 | 128 | 6.88e-34 | 115 |
| MS.gene28935.t1 | AT1G29420 | 57.009 | 107 | 45 | 1 | 1 | 106 | 10 | 116 | 2.96e-33 | 114 |
| MS.gene28935.t1 | AT1G29420 | 57.009 | 107 | 45 | 1 | 1 | 106 | 50 | 156 | 1.25e-32 | 113 |
| MS.gene28935.t1 | AT1G29510 | 54.902 | 102 | 45 | 1 | 5 | 105 | 15 | 116 | 3.88e-32 | 111 |
| MS.gene28935.t1 | AT5G27780 | 55.140 | 107 | 46 | 1 | 1 | 105 | 11 | 117 | 3.98e-32 | 111 |
| MS.gene28935.t1 | AT1G29500 | 51.429 | 105 | 49 | 1 | 1 | 105 | 10 | 112 | 9.18e-32 | 110 |
| MS.gene28935.t1 | AT1G29460 | 42.553 | 141 | 71 | 2 | 1 | 133 | 10 | 148 | 9.47e-32 | 110 |
| MS.gene28935.t1 | AT1G29460 | 42.553 | 141 | 71 | 2 | 1 | 133 | 48 | 186 | 1.02e-31 | 111 |
| MS.gene28935.t1 | AT1G29440 | 45.385 | 130 | 68 | 2 | 5 | 133 | 14 | 141 | 1.47e-29 | 104 |
| MS.gene28935.t1 | AT1G29490 | 52.747 | 91 | 42 | 1 | 1 | 90 | 10 | 100 | 1.79e-24 | 90.5 |
| MS.gene28935.t1 | AT1G76190 | 41.333 | 75 | 41 | 1 | 32 | 103 | 23 | 97 | 4.25e-15 | 67.0 |
| MS.gene28935.t1 | AT1G20470 | 39.394 | 66 | 40 | 0 | 30 | 95 | 23 | 88 | 8.47e-13 | 61.6 |
| MS.gene28935.t1 | AT5G66260 | 39.189 | 74 | 44 | 1 | 20 | 92 | 24 | 97 | 2.55e-11 | 56.6 |
Find 26 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTCTTCATCAGTAAATA+AGG | 0.216090 | 4.4:+34483024 | MS.gene28935:CDS |
| TTCATATCCACTTCGTTTCT+TGG | 0.266340 | 4.4:-34483004 | None:intergenic |
| AAGATCATGACAGGGATTAA+AGG | 0.342898 | 4.4:-34483296 | None:intergenic |
| TTAATCCCTGTCATGATCTT+AGG | 0.345383 | 4.4:+34483299 | MS.gene28935:CDS |
| TGTTTAGAGAACTCTTTAAA+TGG | 0.360343 | 4.4:+34483116 | MS.gene28935:CDS |
| AATGAACAAATAATTATCTA+TGG | 0.367557 | 4.4:+34483328 | MS.gene28935:CDS |
| CTATCACACGGCAACATAAT+AGG | 0.373785 | 4.4:-34483172 | None:intergenic |
| TCATATCCACTTCGTTTCTT+GGG | 0.376786 | 4.4:-34483003 | None:intergenic |
| CAAAGCCTTCTCCACGTCTT+CGG | 0.381612 | 4.4:-34483239 | None:intergenic |
| CATTGATCGCAAGCGATTTG+TGG | 0.397346 | 4.4:+34483066 | MS.gene28935:CDS |
| AAAGCCTTCTCCACGTCTTC+GGG | 0.423323 | 4.4:-34483238 | None:intergenic |
| AACTCTTCATCAGTAAATAA+GGG | 0.445037 | 4.4:+34483025 | MS.gene28935:CDS |
| TTCGTGCTAAGATACTTTAG+AGG | 0.450847 | 4.4:-34483091 | None:intergenic |
| AAATGGTCCGAGGAAGTGTT+CGG | 0.456406 | 4.4:+34483133 | MS.gene28935:CDS |
| GAGGAAGTGTTCGGATTACC+AGG | 0.460604 | 4.4:+34483142 | MS.gene28935:CDS |
| TGGCGGTCGAAAAGCGCAAG+AGG | 0.546936 | 4.4:+34482972 | MS.gene28935:CDS |
| TGGTAATCCGAACACTTCCT+CGG | 0.549919 | 4.4:-34483140 | None:intergenic |
| GATTCCCGAAGACGTGGAGA+AGG | 0.576460 | 4.4:+34483234 | MS.gene28935:CDS |
| GTGTTCGGATTACCAGGGAA+TGG | 0.590996 | 4.4:+34483148 | MS.gene28935:CDS |
| AACATAATAGGTCCATTCCC+TGG | 0.597389 | 4.4:-34483160 | None:intergenic |
| AGAACAGATTCCCGAAGACG+TGG | 0.599588 | 4.4:+34483228 | MS.gene28935:CDS |
| CTCATACCCAAGAAACGAAG+TGG | 0.601207 | 4.4:+34482997 | MS.gene28935:CDS |
| AGAACTCTTTAAATGGTCCG+AGG | 0.631606 | 4.4:+34483123 | MS.gene28935:CDS |
| CATGTATGCTTGTCACAACG+AGG | 0.660738 | 4.4:+34483270 | MS.gene28935:CDS |
| TCCAAGAAAACACTATCACA+CGG | 0.685946 | 4.4:-34483184 | None:intergenic |
| AGGAAGTGTTCGGATTACCA+GGG | 0.722902 | 4.4:+34483143 | MS.gene28935:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAACAAATAATTATCTA+TGG | + | chr4.4:34483328-34483347 | MS.gene28935:CDS | 15.0% |
| ! | AACTCTTCATCAGTAAATAA+GGG | + | chr4.4:34483025-34483044 | MS.gene28935:CDS | 25.0% |
| ! | TGTTTAGAGAACTCTTTAAA+TGG | + | chr4.4:34483116-34483135 | MS.gene28935:CDS | 25.0% |
| GAACTCTTCATCAGTAAATA+AGG | + | chr4.4:34483024-34483043 | MS.gene28935:CDS | 30.0% | |
| AAGATCATGACAGGGATTAA+AGG | - | chr4.4:34483299-34483318 | None:intergenic | 35.0% | |
| TCATATCCACTTCGTTTCTT+GGG | - | chr4.4:34483006-34483025 | None:intergenic | 35.0% | |
| TCCAAGAAAACACTATCACA+CGG | - | chr4.4:34483187-34483206 | None:intergenic | 35.0% | |
| TTAATCCCTGTCATGATCTT+AGG | + | chr4.4:34483299-34483318 | MS.gene28935:CDS | 35.0% | |
| TTCATATCCACTTCGTTTCT+TGG | - | chr4.4:34483007-34483026 | None:intergenic | 35.0% | |
| TTCGTGCTAAGATACTTTAG+AGG | - | chr4.4:34483094-34483113 | None:intergenic | 35.0% | |
| ! | TTTTGCCTAAGATCATGACA+GGG | - | chr4.4:34483307-34483326 | None:intergenic | 35.0% |
| ! | TTTTTGCCTAAGATCATGAC+AGG | - | chr4.4:34483308-34483327 | None:intergenic | 35.0% |
| AACATAATAGGTCCATTCCC+TGG | - | chr4.4:34483163-34483182 | None:intergenic | 40.0% | |
| AGAACTCTTTAAATGGTCCG+AGG | + | chr4.4:34483123-34483142 | MS.gene28935:CDS | 40.0% | |
| CTATCACACGGCAACATAAT+AGG | - | chr4.4:34483175-34483194 | None:intergenic | 40.0% | |
| GAAAAAGTGGCAGAAAATGG+CGG | + | chr4.4:34482955-34482974 | MS.gene28935:CDS | 40.0% | |
| CATGTATGCTTGTCACAACG+AGG | + | chr4.4:34483270-34483289 | MS.gene28935:CDS | 45.0% | |
| CATTGATCGCAAGCGATTTG+TGG | + | chr4.4:34483066-34483085 | MS.gene28935:CDS | 45.0% | |
| CTCATACCCAAGAAACGAAG+TGG | + | chr4.4:34482997-34483016 | MS.gene28935:CDS | 45.0% | |
| GGCGAAAAAGTGGCAGAAAA+TGG | + | chr4.4:34482952-34482971 | MS.gene28935:CDS | 45.0% | |
| TGGTAATCCGAACACTTCCT+CGG | - | chr4.4:34483143-34483162 | None:intergenic | 45.0% | |
| !! | AAATGGTCCGAGGAAGTGTT+CGG | + | chr4.4:34483133-34483152 | MS.gene28935:CDS | 45.0% |
| !! | AGGAAGTGTTCGGATTACCA+GGG | + | chr4.4:34483143-34483162 | MS.gene28935:CDS | 45.0% |
| !! | GCCGTGTGATAGTGTTTTCT+TGG | + | chr4.4:34483183-34483202 | MS.gene28935:CDS | 45.0% |
| AAAGCCTTCTCCACGTCTTC+GGG | - | chr4.4:34483241-34483260 | None:intergenic | 50.0% | |
| AGAACAGATTCCCGAAGACG+TGG | + | chr4.4:34483228-34483247 | MS.gene28935:CDS | 50.0% | |
| CAAAGCCTTCTCCACGTCTT+CGG | - | chr4.4:34483242-34483261 | None:intergenic | 50.0% | |
| GTGTTCGGATTACCAGGGAA+TGG | + | chr4.4:34483148-34483167 | MS.gene28935:CDS | 50.0% | |
| !! | GAGGAAGTGTTCGGATTACC+AGG | + | chr4.4:34483142-34483161 | MS.gene28935:CDS | 50.0% |
| GATTCCCGAAGACGTGGAGA+AGG | + | chr4.4:34483234-34483253 | MS.gene28935:CDS | 55.0% | |
| ! | TGGCGGTCGAAAAGCGCAAG+AGG | + | chr4.4:34482972-34482991 | MS.gene28935:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 34482950 | 34483357 | 34482950 | ID=MS.gene28935 |
| chr4.4 | mRNA | 34482950 | 34483357 | 34482950 | ID=MS.gene28935.t1;Parent=MS.gene28935 |
| chr4.4 | exon | 34482950 | 34483357 | 34482950 | ID=MS.gene28935.t1.exon1;Parent=MS.gene28935.t1 |
| chr4.4 | CDS | 34482950 | 34483357 | 34482950 | ID=cds.MS.gene28935.t1;Parent=MS.gene28935.t1 |
| Gene Sequence |
| Protein sequence |
