Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052293.t1 | RHN61630.1 | 97.8 | 135 | 3 | 0 | 1 | 135 | 10 | 144 | 5.30E-70 | 273.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052293.t1 | Q0V7Z5 | 46.7 | 135 | 67 | 2 | 1 | 133 | 10 | 141 | 6.9e-26 | 118.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052293.t1 | A0A072ULN9 | 97.8 | 135 | 3 | 0 | 1 | 135 | 1 | 135 | 3.9e-70 | 273.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050767 | MS.gene052293 | 0.815222 | 1.03E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene052293 | 0.817037 | 4.06E-52 | -1.69E-46 |
| MS.gene052290 | MS.gene052293 | 0.824672 | 7.11E-54 | -1.69E-46 |
| MS.gene052293 | MS.gene053322 | 0.806958 | 6.39E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene054376 | 0.815306 | 9.89E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene055397 | 0.8111 | 8.29E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene055499 | 0.801442 | 8.99E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene057129 | 0.810619 | 1.05E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene058660 | 0.81721 | 3.71E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene059692 | 0.804714 | 1.89E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene061200 | 0.801524 | 8.64E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene061788 | 0.809933 | 1.48E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene062252 | 0.802435 | 5.62E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene062331 | 0.800622 | 1.32E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene062891 | 0.806078 | 9.80E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene066053 | 0.800287 | 1.55E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene06681 | 0.821064 | 4.93E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene067002 | 0.805962 | 1.04E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene068590 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene070666 | 0.827621 | 1.41E-54 | -1.69E-46 |
| MS.gene052293 | MS.gene071613 | 0.80072 | 1.26E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene072568 | 0.813444 | 2.55E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene072571 | 0.814061 | 1.87E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene073925 | 0.809449 | 1.88E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene07407 | 0.800221 | 1.60E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene09357 | 0.820731 | 5.87E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene21530 | 0.826104 | 3.26E-54 | -1.69E-46 |
| MS.gene052293 | MS.gene22209 | 0.800972 | 1.12E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene22291 | 0.802972 | 4.35E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene23002 | 0.80022 | 1.60E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene23137 | 0.816178 | 6.32E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene23141 | 0.815604 | 8.49E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene23271 | 0.822414 | 2.40E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene23328 | 0.806096 | 9.71E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene23923 | 0.809413 | 1.92E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene25171 | 0.805562 | 1.26E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene26340 | 0.805161 | 1.53E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene27725 | 0.800566 | 1.36E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene28935 | 0.938947 | 2.89E-99 | -1.69E-46 |
| MS.gene052293 | MS.gene30766 | 0.817254 | 3.63E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene33855 | 0.800382 | 1.48E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene33873 | 0.803614 | 3.21E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene34231 | 0.81893 | 1.52E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene34454 | 0.814814 | 1.27E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene36648 | 0.814541 | 1.46E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene40233 | 0.829205 | 5.87E-55 | -1.69E-46 |
| MS.gene052293 | MS.gene46602 | 0.814726 | 1.33E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene49257 | 0.82065 | 6.13E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene50731 | 0.815757 | 7.85E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene52238 | 0.802963 | 4.37E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene55937 | 0.809641 | 1.71E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene57299 | 0.801684 | 8.01E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene57406 | 0.803384 | 3.58E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene64148 | 0.800944 | 1.14E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene64414 | 0.832944 | 7.08E-56 | -1.69E-46 |
| MS.gene052293 | MS.gene66956 | 0.80376 | 2.99E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene68129 | 0.810345 | 1.21E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene68130 | 0.803751 | 3.00E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene68178 | 0.81974 | 9.91E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene68729 | 0.806988 | 6.29E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene69489 | 0.800874 | 1.17E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene69810 | 0.814417 | 1.56E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene70093 | 0.803131 | 4.03E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene70095 | 0.801788 | 7.63E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene71024 | 0.800098 | 1.69E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene71604 | 0.806041 | 9.97E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene71829 | 0.807358 | 5.26E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene76306 | 0.821399 | 4.12E-53 | -1.69E-46 |
| MS.gene052293 | MS.gene79762 | 0.801439 | 9.00E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene79806 | 0.805083 | 1.58E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene81793 | 0.800413 | 1.46E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene85449 | 0.815272 | 1.01E-51 | -1.69E-46 |
| MS.gene052293 | MS.gene85579 | 0.802844 | 4.63E-49 | -1.69E-46 |
| MS.gene052293 | MS.gene91319 | 0.817454 | 3.27E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene91325 | 0.808074 | 3.70E-50 | -1.69E-46 |
| MS.gene052293 | MS.gene92659 | 0.817394 | 3.37E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene92661 | 0.826427 | 2.73E-54 | -1.69E-46 |
| MS.gene052293 | MS.gene96705 | 0.800862 | 1.18E-48 | -1.69E-46 |
| MS.gene052293 | MS.gene96838 | 0.81814 | 2.29E-52 | -1.69E-46 |
| MS.gene052293 | MS.gene99139 | 0.802113 | 6.54E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene052293 | MS.gene28935 | PPI |
| MS.gene052293 | MS.gene003386 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052293.t1 | MTR_4g075760 | 97.778 | 135 | 3 | 0 | 1 | 135 | 1 | 135 | 1.31e-97 | 276 |
| MS.gene052293.t1 | MTR_4g072910 | 39.326 | 89 | 47 | 4 | 9 | 94 | 14 | 98 | 3.90e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052293.t1 | AT1G29450 | 46.667 | 135 | 67 | 2 | 1 | 133 | 10 | 141 | 3.92e-35 | 119 |
| MS.gene052293.t1 | AT1G29450 | 46.667 | 135 | 67 | 2 | 1 | 133 | 10 | 141 | 3.92e-35 | 119 |
| MS.gene052293.t1 | AT1G29420 | 53.279 | 122 | 54 | 2 | 1 | 119 | 10 | 131 | 6.81e-34 | 115 |
| MS.gene052293.t1 | AT1G29430 | 53.922 | 102 | 46 | 1 | 5 | 105 | 15 | 116 | 1.94e-33 | 114 |
| MS.gene052293.t1 | AT1G29420 | 53.279 | 122 | 54 | 2 | 1 | 119 | 50 | 171 | 4.04e-33 | 115 |
| MS.gene052293.t1 | AT1G29510 | 45.736 | 129 | 68 | 2 | 5 | 132 | 15 | 142 | 2.28e-32 | 112 |
| MS.gene052293.t1 | AT5G27780 | 55.140 | 107 | 46 | 1 | 1 | 105 | 11 | 117 | 3.73e-32 | 111 |
| MS.gene052293.t1 | AT1G29500 | 51.429 | 105 | 49 | 1 | 1 | 105 | 10 | 112 | 8.89e-32 | 110 |
| MS.gene052293.t1 | AT1G29460 | 49.558 | 113 | 49 | 1 | 1 | 105 | 10 | 122 | 8.61e-31 | 108 |
| MS.gene052293.t1 | AT1G29460 | 49.558 | 113 | 49 | 1 | 1 | 105 | 48 | 160 | 9.03e-31 | 108 |
| MS.gene052293.t1 | AT1G29440 | 53.922 | 102 | 46 | 1 | 5 | 105 | 14 | 115 | 2.95e-28 | 101 |
| MS.gene052293.t1 | AT1G29490 | 52.747 | 91 | 42 | 1 | 1 | 90 | 10 | 100 | 1.62e-24 | 90.5 |
| MS.gene052293.t1 | AT1G76190 | 41.333 | 75 | 41 | 1 | 32 | 103 | 23 | 97 | 3.86e-15 | 67.4 |
| MS.gene052293.t1 | AT1G20470 | 39.394 | 66 | 40 | 0 | 30 | 95 | 23 | 88 | 8.21e-13 | 61.6 |
| MS.gene052293.t1 | AT5G66260 | 39.189 | 74 | 44 | 1 | 20 | 92 | 24 | 97 | 2.36e-11 | 57.0 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTCTTCATCAGTAAATA+AGG | 0.216090 | 4.2:+33227570 | MS.gene052293:CDS |
| TTCATATCCACTTCGTTTCT+TGG | 0.266340 | 4.2:-33227550 | None:intergenic |
| TTAATCTCTGTCATGATCTT+AGG | 0.329706 | 4.2:+33227845 | MS.gene052293:CDS |
| AAGATCATGACAGAGATTAA+AGG | 0.359430 | 4.2:-33227842 | None:intergenic |
| TGTTTAGAGAACTCTTTAAA+TGG | 0.360343 | 4.2:+33227662 | MS.gene052293:CDS |
| AATGAACAAATAATTATCTA+TGG | 0.367557 | 4.2:+33227874 | MS.gene052293:CDS |
| CTATCACACGGCAACATAAT+AGG | 0.373785 | 4.2:-33227718 | None:intergenic |
| TCATATCCACTTCGTTTCTT+GGG | 0.376786 | 4.2:-33227549 | None:intergenic |
| CAAAGCCTTCTCCACGTCTT+CGG | 0.381612 | 4.2:-33227785 | None:intergenic |
| CATTGATCGCAAGCGATTTG+TGG | 0.397346 | 4.2:+33227612 | MS.gene052293:CDS |
| AAAGCCTTCTCCACGTCTTC+GGG | 0.423323 | 4.2:-33227784 | None:intergenic |
| AACTCTTCATCAGTAAATAA+GGG | 0.445037 | 4.2:+33227571 | MS.gene052293:CDS |
| TTCGTGCTAAGATACTTTAG+AGG | 0.450847 | 4.2:-33227637 | None:intergenic |
| AAATGGTCCGAGGAAGTGTT+CGG | 0.456406 | 4.2:+33227679 | MS.gene052293:CDS |
| GAGGAAGTGTTCGGATTACC+AGG | 0.460604 | 4.2:+33227688 | MS.gene052293:CDS |
| TGGCGGTCGAAAAGCGCAAG+AGG | 0.546936 | 4.2:+33227518 | MS.gene052293:CDS |
| TGGTAATCCGAACACTTCCT+CGG | 0.549919 | 4.2:-33227686 | None:intergenic |
| GATTCCCGAAGACGTGGAGA+AGG | 0.576460 | 4.2:+33227780 | MS.gene052293:CDS |
| GTGTTCGGATTACCAGGGAA+TGG | 0.590996 | 4.2:+33227694 | MS.gene052293:CDS |
| AACATAATAGGTCCATTCCC+TGG | 0.597389 | 4.2:-33227706 | None:intergenic |
| AGAACAGATTCCCGAAGACG+TGG | 0.599588 | 4.2:+33227774 | MS.gene052293:CDS |
| CTCATACCCAAGAAACGAAG+TGG | 0.601207 | 4.2:+33227543 | MS.gene052293:CDS |
| AGAACTCTTTAAATGGTCCG+AGG | 0.631606 | 4.2:+33227669 | MS.gene052293:CDS |
| CATGTATGCTTGTCACAACG+AGG | 0.660738 | 4.2:+33227816 | MS.gene052293:CDS |
| TCCAAGAAAACACTATCACA+CGG | 0.685946 | 4.2:-33227730 | None:intergenic |
| AGGAAGTGTTCGGATTACCA+GGG | 0.722902 | 4.2:+33227689 | MS.gene052293:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAACAAATAATTATCTA+TGG | + | chr4.2:33227874-33227893 | MS.gene052293:CDS | 15.0% |
| ! | AACTCTTCATCAGTAAATAA+GGG | + | chr4.2:33227571-33227590 | MS.gene052293:CDS | 25.0% |
| ! | TGTTTAGAGAACTCTTTAAA+TGG | + | chr4.2:33227662-33227681 | MS.gene052293:CDS | 25.0% |
| AAGATCATGACAGAGATTAA+AGG | - | chr4.2:33227845-33227864 | None:intergenic | 30.0% | |
| GAACTCTTCATCAGTAAATA+AGG | + | chr4.2:33227570-33227589 | MS.gene052293:CDS | 30.0% | |
| TTAATCTCTGTCATGATCTT+AGG | + | chr4.2:33227845-33227864 | MS.gene052293:CDS | 30.0% | |
| TCATATCCACTTCGTTTCTT+GGG | - | chr4.2:33227552-33227571 | None:intergenic | 35.0% | |
| TCCAAGAAAACACTATCACA+CGG | - | chr4.2:33227733-33227752 | None:intergenic | 35.0% | |
| TTCATATCCACTTCGTTTCT+TGG | - | chr4.2:33227553-33227572 | None:intergenic | 35.0% | |
| TTCGTGCTAAGATACTTTAG+AGG | - | chr4.2:33227640-33227659 | None:intergenic | 35.0% | |
| AACATAATAGGTCCATTCCC+TGG | - | chr4.2:33227709-33227728 | None:intergenic | 40.0% | |
| AGAACTCTTTAAATGGTCCG+AGG | + | chr4.2:33227669-33227688 | MS.gene052293:CDS | 40.0% | |
| CTATCACACGGCAACATAAT+AGG | - | chr4.2:33227721-33227740 | None:intergenic | 40.0% | |
| GAAAAAGTGGCAGAAAATGG+CGG | + | chr4.2:33227501-33227520 | MS.gene052293:CDS | 40.0% | |
| CATGTATGCTTGTCACAACG+AGG | + | chr4.2:33227816-33227835 | MS.gene052293:CDS | 45.0% | |
| CATTGATCGCAAGCGATTTG+TGG | + | chr4.2:33227612-33227631 | MS.gene052293:CDS | 45.0% | |
| CTCATACCCAAGAAACGAAG+TGG | + | chr4.2:33227543-33227562 | MS.gene052293:CDS | 45.0% | |
| GGCGAAAAAGTGGCAGAAAA+TGG | + | chr4.2:33227498-33227517 | MS.gene052293:CDS | 45.0% | |
| TGGTAATCCGAACACTTCCT+CGG | - | chr4.2:33227689-33227708 | None:intergenic | 45.0% | |
| !! | AAATGGTCCGAGGAAGTGTT+CGG | + | chr4.2:33227679-33227698 | MS.gene052293:CDS | 45.0% |
| !! | AGGAAGTGTTCGGATTACCA+GGG | + | chr4.2:33227689-33227708 | MS.gene052293:CDS | 45.0% |
| !! | GCCGTGTGATAGTGTTTTCT+TGG | + | chr4.2:33227729-33227748 | MS.gene052293:CDS | 45.0% |
| AAAGCCTTCTCCACGTCTTC+GGG | - | chr4.2:33227787-33227806 | None:intergenic | 50.0% | |
| AGAACAGATTCCCGAAGACG+TGG | + | chr4.2:33227774-33227793 | MS.gene052293:CDS | 50.0% | |
| CAAAGCCTTCTCCACGTCTT+CGG | - | chr4.2:33227788-33227807 | None:intergenic | 50.0% | |
| GTGTTCGGATTACCAGGGAA+TGG | + | chr4.2:33227694-33227713 | MS.gene052293:CDS | 50.0% | |
| !! | GAGGAAGTGTTCGGATTACC+AGG | + | chr4.2:33227688-33227707 | MS.gene052293:CDS | 50.0% |
| GATTCCCGAAGACGTGGAGA+AGG | + | chr4.2:33227780-33227799 | MS.gene052293:CDS | 55.0% | |
| ! | TGGCGGTCGAAAAGCGCAAG+AGG | + | chr4.2:33227518-33227537 | MS.gene052293:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 33227496 | 33227903 | 33227496 | ID=MS.gene052293 |
| chr4.2 | mRNA | 33227496 | 33227903 | 33227496 | ID=MS.gene052293.t1;Parent=MS.gene052293 |
| chr4.2 | exon | 33227496 | 33227903 | 33227496 | ID=MS.gene052293.t1.exon1;Parent=MS.gene052293.t1 |
| chr4.2 | CDS | 33227496 | 33227903 | 33227496 | ID=cds.MS.gene052293.t1;Parent=MS.gene052293.t1 |
| Gene Sequence |
| Protein sequence |
