AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003415

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003415.t1 MTR_4g075250 95.904 415 13 2 1 415 1 411 0.0 809
MS.gene003415.t1 MTR_5g075880 65.414 133 45 1 28 159 38 170 6.15e-51 170
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003415.t1 AT3G12400 58.907 421 139 5 1 411 1 397 1.04e-162 463
MS.gene003415.t1 AT3G12400 58.852 418 138 5 1 408 1 394 1.87e-161 460
MS.gene003415.t1 AT3G12400 58.852 418 138 5 1 408 1 394 3.38e-161 461
MS.gene003415.t1 AT5G13860 57.246 414 127 6 1 411 1 367 1.03e-155 444
MS.gene003415.t1 AT2G38830 36.025 161 96 4 12 168 12 169 5.10e-25 105
MS.gene003415.t1 AT2G38830 28.947 228 125 7 190 386 96 317 2.79e-16 79.7

Find 154 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATCAAAGTTCTAATTT+TGG 0.132572 4.1:+28553838 MS.gene003415:CDS
CTGAGGAATTGTTGGGTTTC+GGG 0.148763 4.1:-28553307 None:intergenic
TTAGAACTTTGATTGGTATT+TGG 0.178300 4.1:-28553832 None:intergenic
AATTGTTGGGTTTCGGGGTT+TGG 0.182716 4.1:-28553301 None:intergenic
GATTTACCCTAGTTCTAATT+TGG 0.183622 4.1:+28553684 MS.gene003415:CDS
CATCCTCATGTTAATCCTTC+TGG 0.204058 4.1:+28553631 MS.gene003415:CDS
CCCAATTGTTATCTGGCTTA+TGG 0.211522 4.1:+28553540 MS.gene003415:CDS
ATTGTTGGGTTTCGGGGTTT+GGG 0.228262 4.1:-28553300 None:intergenic
GTGAGTGTAGGTGGCGGTTT+TGG 0.235521 4.1:-28553440 None:intergenic
GCTGAGGAATTGTTGGGTTT+CGG 0.263335 4.1:-28553308 None:intergenic
CTGTTCCGTATTTGCATAAC+TGG 0.268788 4.1:+28553662 MS.gene003415:CDS
TGCTTTAGATAAGGGTGTTC+AGG 0.272232 4.1:+28554356 MS.gene003415:CDS
GGAGAATCAAGGGAAGAAAA+TGG 0.279130 4.1:+28554230 MS.gene003415:CDS
GCAGGCTCAGGTTGCGAATA+TGG 0.279429 4.1:+28554482 MS.gene003415:CDS
TAGATAAGGGTGTTCAGGTT+GGG 0.287353 4.1:+28554361 MS.gene003415:CDS
CGAGATGGCGCAGGGGAATA+AGG 0.290860 4.1:-28553943 None:intergenic
AGGGAAGAAAATGGGGAGTT+TGG 0.299911 4.1:+28554239 MS.gene003415:CDS
CTTTGATTGGTATTTGGATT+AGG 0.305114 4.1:-28553826 None:intergenic
TGATTGTACTTCTGCTGATT+TGG 0.313474 4.1:+28554314 MS.gene003415:CDS
TTGTTGGGTTTCGGGGTTTG+GGG 0.329175 4.1:-28553299 None:intergenic
ACAACATCCCAATTGTTATC+TGG 0.333242 4.1:+28553533 MS.gene003415:CDS
TTAGGGTTAGGGTTAGGATT+AGG 0.335904 4.1:-28553784 None:intergenic
GAGAATCAAGGGAAGAAAAT+GGG 0.338918 4.1:+28554231 MS.gene003415:CDS
TTTGATGAATACGGATGTTC+TGG 0.345033 4.1:+28554194 MS.gene003415:CDS
TTAGGATTAGGATTGGGATT+AGG 0.352631 4.1:-28553772 None:intergenic
TCCAAAATTAGAACTTTGAT+TGG 0.354530 4.1:-28553839 None:intergenic
GGTGAAATGGAGGGGTTGTT+TGG 0.355099 4.1:+28554072 MS.gene003415:CDS
TTTGGAACTAATCCTCCATC+TGG 0.359655 4.1:+28553856 MS.gene003415:CDS
AGTTAGAGCTGCTCAGTTGC+AGG 0.360251 4.1:+28554464 MS.gene003415:CDS
ACGCTTTATGCTTTAGATAA+GGG 0.364897 4.1:+28554348 MS.gene003415:CDS
TCCATAAGCCAGATAACAAT+TGG 0.369758 4.1:-28553541 None:intergenic
GCAAGATGAGATGGAGGGTT+TGG 0.370726 4.1:+28554152 MS.gene003415:CDS
GAAGACTAGAGAAGGTGAAA+TGG 0.378989 4.1:+28554059 MS.gene003415:CDS
AGCTGCTCAGTTGCAGGCTC+AGG 0.393395 4.1:+28554470 MS.gene003415:CDS
AGATAAGGGTGTTCAGGTTG+GGG 0.397945 4.1:+28554362 MS.gene003415:CDS
TTTGATGAGGATTAGGGTTA+GGG 0.399829 4.1:-28553795 None:intergenic
CATTTGGTGTCTTCGGAGTA+AGG 0.400855 4.1:-28553364 None:intergenic
TACGCTTTATGCTTTAGATA+AGG 0.408278 4.1:+28554347 MS.gene003415:CDS
ATGTTCTGGAGGGTTGGTTG+AGG 0.410858 4.1:+28554208 MS.gene003415:CDS
GAGAGCGGAGGGGCCGCGTT+GGG 0.413398 4.1:-28553341 None:intergenic
GGATTGAGCTGAGGAATTGT+TGG 0.417142 4.1:-28553315 None:intergenic
TCATGTTAATCCTTCTGGAT+TGG 0.419158 4.1:+28553636 MS.gene003415:CDS
GCTGCTAGGAATCATCATTA+TGG 0.423124 4.1:+28554504 MS.gene003415:CDS
TGAGGATTAGGGTTAGGGTT+AGG 0.423347 4.1:-28553790 None:intergenic
GGTATTGAAGCAGAGGGAGG+AGG 0.425612 4.1:+28554104 MS.gene003415:CDS
GAGGGGTTGTTTGGTTTGCA+AGG 0.429772 4.1:+28554081 MS.gene003415:CDS
AGTACAATCAAGCATCTGTT+TGG 0.430905 4.1:-28554301 None:intergenic
TACCGTTTGATCAGTATTTG+AGG 0.441585 4.1:+28554388 MS.gene003415:CDS
GGAAGGTGGTGAGGAGAGAG+AGG 0.450712 4.1:-28553405 None:intergenic
TAAATCCAGTTATGCAAATA+CGG 0.451965 4.1:-28553667 None:intergenic
GATTGAGCTGAGGAATTGTT+GGG 0.455019 4.1:-28553314 None:intergenic
AGGGGTTGTTTGGTTTGCAA+GGG 0.461101 4.1:+28554082 MS.gene003415:CDS
CTCCTCAAATACTGATCAAA+CGG 0.461994 4.1:-28554390 None:intergenic
CGGAGGGGCCGCGTTGGGAG+AGG 0.462809 4.1:-28553336 None:intergenic
GCCATCTCGGCCTACGCATA+CGG 0.464143 4.1:+28553957 MS.gene003415:CDS
GGAGAGAGAGGAGATGTTGA+CGG 0.466235 4.1:-28553393 None:intergenic
TGTTCTGGAGGGTTGGTTGA+GGG 0.466933 4.1:+28554209 MS.gene003415:CDS
GATAAGCCATTTGGTGTCTT+CGG 0.468483 4.1:-28553371 None:intergenic
GTTAGGGTTAGGATTAGGAT+TGG 0.469730 4.1:-28553779 None:intergenic
TTAGGGTTAGGATTAGGATT+GGG 0.470431 4.1:-28553778 None:intergenic
CTTATTCCCCTGCGCCATCT+CGG 0.471542 4.1:+28553944 MS.gene003415:CDS
GGAGATGCAAGATGAGATGG+AGG 0.478519 4.1:+28554146 MS.gene003415:CDS
TTTAATAATCATATCCCTAG+TGG 0.481041 4.1:-28553602 None:intergenic
GACTAGAGAAGGTGAAATGG+AGG 0.483262 4.1:+28554062 MS.gene003415:CDS
GGATTGTGCCATCGGCTTGA+AGG 0.490820 4.1:-28553486 None:intergenic
CGAGAGAAGGGAAGGTGGTG+AGG 0.490894 4.1:-28553414 None:intergenic
TTAGATAAGGGTGTTCAGGT+TGG 0.491802 4.1:+28554360 MS.gene003415:CDS
GGGGTTGTTTGGTTTGCAAG+GGG 0.495332 4.1:+28554083 MS.gene003415:CDS
GGTTGGTTGAGGGAGAATCA+AGG 0.499129 4.1:+28554219 MS.gene003415:CDS
TGCAAGGGGTATTGAAGCAG+AGG 0.500833 4.1:+28554097 MS.gene003415:CDS
GGAGAGCGGAGGGGCCGCGT+TGG 0.502152 4.1:-28553342 None:intergenic
CCATCTGGGTATATTCATCC+TGG 0.504818 4.1:+28553871 MS.gene003415:CDS
TCGGAGTAAGGGAGAGCGGA+GGG 0.508533 4.1:-28553352 None:intergenic
ATGTTGACGGATAAGCCATT+TGG 0.510171 4.1:-28553380 None:intergenic
GGTCATGGGGATTGTGCCAT+CGG 0.512307 4.1:-28553494 None:intergenic
AGATCAACCAAATTAGAACT+AGG 0.514058 4.1:-28553691 None:intergenic
GTTGGTTGAGGGAGAATCAA+GGG 0.518369 4.1:+28554220 MS.gene003415:CDS
CTTACTCCGAAGACACCAAA+TGG 0.520060 4.1:+28553365 MS.gene003415:CDS
CTATTACTATTAATCCTACC+AGG 0.522290 4.1:-28553889 None:intergenic
TGCGTAGGCCGAGATGGCGC+AGG 0.522751 4.1:-28553952 None:intergenic
GAGTGTGAGGGCGTTGTCGA+GGG 0.528924 4.1:+28554410 MS.gene003415:CDS
TGTTGGGTTTCGGGGTTTGG+GGG 0.530033 4.1:-28553298 None:intergenic
CCATAAGCCAGATAACAATT+GGG 0.531355 4.1:-28553540 None:intergenic
GTGACGGATTGGTAGGTCAT+GGG 0.535180 4.1:-28553508 None:intergenic
GAAGGAGATGCAAGATGAGA+TGG 0.537662 4.1:+28554143 MS.gene003415:CDS
AGAATCAAGGGAAGAAAATG+GGG 0.538001 4.1:+28554232 MS.gene003415:CDS
GGGTTCGAGAGAAGGGAAGG+TGG 0.544802 4.1:-28553419 None:intergenic
ACTAGAGAAGGTGAAATGGA+GGG 0.549010 4.1:+28554063 MS.gene003415:CDS
TTTGGGTTCGAGAGAAGGGA+AGG 0.549016 4.1:-28553422 None:intergenic
TCCGTATGCGTAGGCCGAGA+TGG 0.550136 4.1:-28553958 None:intergenic
GTCAATCTCCTTCAAGCCGA+TGG 0.554176 4.1:+28553478 MS.gene003415:CDS
CTAGAGAAGGTGAAATGGAG+GGG 0.556264 4.1:+28554064 MS.gene003415:CDS
TTTGGAGCAGCAGTTGCAGA+TGG 0.557427 4.1:+28554170 MS.gene003415:CDS
GCAGTTCTTCCACCGTGCTA+CGG 0.559208 4.1:+28554434 MS.gene003415:CDS
ACGGCCGTCGTTGTGAGTGT+AGG 0.560561 4.1:-28553452 None:intergenic
GGGGAGTTTGGAGAATGTTG+AGG 0.560828 4.1:+28554251 MS.gene003415:CDS
ATCAGTATTTGAGGAGTGTG+AGG 0.563552 4.1:+28554397 MS.gene003415:CDS
TTAATAATCATATCCCTAGT+GGG 0.565302 4.1:-28553601 None:intergenic
GATCAACCAAATTAGAACTA+GGG 0.566055 4.1:-28553690 None:intergenic
GGAGTGTGAGGGCGTTGTCG+AGG 0.566472 4.1:+28554409 MS.gene003415:CDS
TATTAACAAGCTTGTGGAGA+TGG 0.569223 4.1:+28554011 MS.gene003415:CDS
GGATGTTGTATGTGACGGAT+TGG 0.574702 4.1:-28553519 None:intergenic
GTGTTTATGTTAATCCCACT+AGG 0.576064 4.1:+28553587 MS.gene003415:CDS
GCCGTCGTTGTGAGTGTAGG+TGG 0.582716 4.1:-28553449 None:intergenic
TGAGGAATTGTTGGGTTTCG+GGG 0.583538 4.1:-28553306 None:intergenic
GAGTTTGGAGAATGTTGAGG+AGG 0.585981 4.1:+28554254 MS.gene003415:CDS
ATTTGGTGTCTTCGGAGTAA+GGG 0.586991 4.1:-28553363 None:intergenic
TTGGAACTAATCCTCCATCT+GGG 0.587137 4.1:+28553857 MS.gene003415:CDS
AGGTTGCGAATATGGCTGCT+AGG 0.587435 4.1:+28554490 MS.gene003415:CDS
GCGTAGGCCGAGATGGCGCA+GGG 0.587625 4.1:-28553951 None:intergenic
GATGAATACGGATGTTCTGG+AGG 0.588358 4.1:+28554197 MS.gene003415:CDS
ATCGGCTTGAAGGAGATTGA+CGG 0.588553 4.1:-28553476 None:intergenic
GCAGGGGAATAAGGCGAAGG+AGG 0.592529 4.1:-28553934 None:intergenic
GTTGTATGTGACGGATTGGT+AGG 0.598464 4.1:-28553515 None:intergenic
GAATGCTATTAACAAGCTTG+TGG 0.599733 4.1:+28554005 MS.gene003415:CDS
TGTTTATGTTAATCCCACTA+GGG 0.610790 4.1:+28553588 MS.gene003415:CDS
TGTGACGGATTGGTAGGTCA+TGG 0.615822 4.1:-28553509 None:intergenic
TTCGGAGTAAGGGAGAGCGG+AGG 0.618360 4.1:-28553353 None:intergenic
AATCATCATTATGGTAGCTG+AGG 0.620092 4.1:+28554513 MS.gene003415:CDS
ATACGGATGTTCTGGAGGGT+TGG 0.622239 4.1:+28554202 MS.gene003415:CDS
TAAACACGAGGAGGGTGACG+AGG 0.622293 4.1:-28553571 None:intergenic
GTCGTTGTGAGTGTAGGTGG+CGG 0.624469 4.1:-28553446 None:intergenic
TAACTTTAGCAGCCGTAGCA+CGG 0.627369 4.1:-28554446 None:intergenic
AGGGGTATTGAAGCAGAGGG+AGG 0.628332 4.1:+28554101 MS.gene003415:CDS
GTCTTCGGAGTAAGGGAGAG+CGG 0.628756 4.1:-28553356 None:intergenic
CTTGAAGGAGATTGACGGCA+CGG 0.628941 4.1:-28553471 None:intergenic
GCGTTGAGGAAGACTAGAGA+AGG 0.630568 4.1:+28554051 MS.gene003415:CDS
CTGGGTATATTCATCCTGGT+AGG 0.631659 4.1:+28553875 MS.gene003415:CDS
GGCGCAGGGGAATAAGGCGA+AGG 0.637625 4.1:-28553937 None:intergenic
TGACGGATTGGTAGGTCATG+GGG 0.639394 4.1:-28553507 None:intergenic
GCCACCTACACTCACAACGA+CGG 0.639409 4.1:+28553448 MS.gene003415:CDS
CCAGGATGAATATACCCAGA+TGG 0.642839 4.1:-28553871 None:intergenic
TCAGTATTTGAGGAGTGTGA+GGG 0.644098 4.1:+28554398 MS.gene003415:CDS
GGAACAGAAACCAATCCAGA+AGG 0.646246 4.1:-28553646 None:intergenic
AATTGGGATGTTGTATGTGA+CGG 0.646465 4.1:-28553524 None:intergenic
GTAGGATCCTCCGTATGCGT+AGG 0.651752 4.1:-28553967 None:intergenic
GAGATGCAAGATGAGATGGA+GGG 0.654104 4.1:+28554147 MS.gene003415:CDS
ATGAATACGGATGTTCTGGA+GGG 0.655370 4.1:+28554198 MS.gene003415:CDS
GGATTAACATAAACACGAGG+AGG 0.659983 4.1:-28553580 None:intergenic
ATCTCGGCCTACGCATACGG+AGG 0.661157 4.1:+28553960 MS.gene003415:CDS
GCAAGGGGTATTGAAGCAGA+GGG 0.664131 4.1:+28554098 MS.gene003415:CDS
AAACACGAGGAGGGTGACGA+GGG 0.665903 4.1:-28553570 None:intergenic
CGGAGTAAGGGAGAGCGGAG+GGG 0.668045 4.1:-28553351 None:intergenic
GATGGTGCATAATGATGTGA+CGG 0.671850 4.1:+28554029 MS.gene003415:CDS
AATCCAGAAGGATTAACATG+AGG 0.672810 4.1:-28553634 None:intergenic
GGATGAATATACCCAGATGG+AGG 0.673938 4.1:-28553868 None:intergenic
GTTGGGAGAGGATTGAGCTG+AGG 0.683317 4.1:-28553324 None:intergenic
ATAATGATGTGACGGCGTTG+AGG 0.684564 4.1:+28554037 MS.gene003415:CDS
CGTAGGCCGAGATGGCGCAG+GGG 0.702882 4.1:-28553950 None:intergenic
AGCTCAATCCTCTCCCAACG+CGG 0.705583 4.1:+28553328 MS.gene003415:CDS
GATTAACATAAACACGAGGA+GGG 0.706994 4.1:-28553579 None:intergenic
CTTTAGCAGCCGTAGCACGG+TGG 0.725711 4.1:-28554443 None:intergenic
GCATACGGAGGATCCTACTG+AGG 0.760419 4.1:+28553972 MS.gene003415:CDS
GTGGGATTAACATAAACACG+AGG 0.780290 4.1:-28553583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAATCCAGTTATGCAAATA+CGG - chr4.1:28553670-28553689 None:intergenic 25.0%
! TCCAAAATTAGAACTTTGAT+TGG - chr4.1:28553842-28553861 None:intergenic 25.0%
! TTAATAATCATATCCCTAGT+GGG - chr4.1:28553604-28553623 None:intergenic 25.0%
! TTAGAACTTTGATTGGTATT+TGG - chr4.1:28553835-28553854 None:intergenic 25.0%
! TTTAATAATCATATCCCTAG+TGG - chr4.1:28553605-28553624 None:intergenic 25.0%
!!! ACCAATCAAAGTTCTAATTT+TGG + chr4.1:28553838-28553857 MS.gene003415:CDS 25.0%
!!! TATGATTTTGATGAGGATTA+GGG - chr4.1:28553804-28553823 None:intergenic 25.0%
!!! TTAGGATTATGATTTTGATG+AGG - chr4.1:28553811-28553830 None:intergenic 25.0%
!!! TTATGATTTTGATGAGGATT+AGG - chr4.1:28553805-28553824 None:intergenic 25.0%
AGATCAACCAAATTAGAACT+AGG - chr4.1:28553694-28553713 None:intergenic 30.0%
CTATTACTATTAATCCTACC+AGG - chr4.1:28553892-28553911 None:intergenic 30.0%
CTTTGATTGGTATTTGGATT+AGG - chr4.1:28553829-28553848 None:intergenic 30.0%
GATCAACCAAATTAGAACTA+GGG - chr4.1:28553693-28553712 None:intergenic 30.0%
TGTTTATGTTAATCCCACTA+GGG + chr4.1:28553588-28553607 MS.gene003415:CDS 30.0%
! ACGCTTTATGCTTTAGATAA+GGG + chr4.1:28554348-28554367 MS.gene003415:CDS 30.0%
! TACGCTTTATGCTTTAGATA+AGG + chr4.1:28554347-28554366 MS.gene003415:CDS 30.0%
!! CTTGCTTTGAGTTTGATTTT+TGG + chr4.1:28553718-28553737 MS.gene003415:CDS 30.0%
!! GATTTACCCTAGTTCTAATT+TGG + chr4.1:28553684-28553703 MS.gene003415:CDS 30.0%
AATCCAGAAGGATTAACATG+AGG - chr4.1:28553637-28553656 None:intergenic 35.0%
AATTGGGATGTTGTATGTGA+CGG - chr4.1:28553527-28553546 None:intergenic 35.0%
ACAACATCCCAATTGTTATC+TGG + chr4.1:28553533-28553552 MS.gene003415:CDS 35.0%
AGAATCAAGGGAAGAAAATG+GGG + chr4.1:28554232-28554251 MS.gene003415:CDS 35.0%
AGTACAATCAAGCATCTGTT+TGG - chr4.1:28554304-28554323 None:intergenic 35.0%
CCATAAGCCAGATAACAATT+GGG - chr4.1:28553543-28553562 None:intergenic 35.0%
CTCCTCAAATACTGATCAAA+CGG - chr4.1:28554393-28554412 None:intergenic 35.0%
GAATGCTATTAACAAGCTTG+TGG + chr4.1:28554005-28554024 MS.gene003415:CDS 35.0%
GAGAATCAAGGGAAGAAAAT+GGG + chr4.1:28554231-28554250 MS.gene003415:CDS 35.0%
GATTAACATAAACACGAGGA+GGG - chr4.1:28553582-28553601 None:intergenic 35.0%
GTGTTTATGTTAATCCCACT+AGG + chr4.1:28553587-28553606 MS.gene003415:CDS 35.0%
TACCGTTTGATCAGTATTTG+AGG + chr4.1:28554388-28554407 MS.gene003415:CDS 35.0%
TATTAACAAGCTTGTGGAGA+TGG + chr4.1:28554011-28554030 MS.gene003415:CDS 35.0%
TCATGTTAATCCTTCTGGAT+TGG + chr4.1:28553636-28553655 MS.gene003415:CDS 35.0%
TCCATAAGCCAGATAACAAT+TGG - chr4.1:28553544-28553563 None:intergenic 35.0%
TTAGGATTAGGATTGGGATT+AGG - chr4.1:28553775-28553794 None:intergenic 35.0%
TTAGGGTTAGGATTAGGATT+GGG - chr4.1:28553781-28553800 None:intergenic 35.0%
TTTGATGAATACGGATGTTC+TGG + chr4.1:28554194-28554213 MS.gene003415:CDS 35.0%
TTTGATGAGGATTAGGGTTA+GGG - chr4.1:28553798-28553817 None:intergenic 35.0%
! TGATTGTACTTCTGCTGATT+TGG + chr4.1:28554314-28554333 MS.gene003415:CDS 35.0%
! TTTTGATGAGGATTAGGGTT+AGG - chr4.1:28553799-28553818 None:intergenic 35.0%
!!! GCAGATGGTTTTGATGAATA+CGG + chr4.1:28554185-28554204 MS.gene003415:CDS 35.0%
!!! GGTTTTGAATAATGGTGTGA+AGG + chr4.1:28554125-28554144 MS.gene003415:CDS 35.0%
ACTAGAGAAGGTGAAATGGA+GGG + chr4.1:28554063-28554082 MS.gene003415:CDS 40.0%
CATCCTCATGTTAATCCTTC+TGG + chr4.1:28553631-28553650 MS.gene003415:CDS 40.0%
CTACGTTGAGAAAAAAGAGG+TGG - chr4.1:28553751-28553770 None:intergenic 40.0%
CTGTTCCGTATTTGCATAAC+TGG + chr4.1:28553662-28553681 MS.gene003415:CDS 40.0%
GAAGACTAGAGAAGGTGAAA+TGG + chr4.1:28554059-28554078 MS.gene003415:CDS 40.0%
GATGGTGCATAATGATGTGA+CGG + chr4.1:28554029-28554048 MS.gene003415:CDS 40.0%
GATTGAGCTGAGGAATTGTT+GGG - chr4.1:28553317-28553336 None:intergenic 40.0%
GCTGCTAGGAATCATCATTA+TGG + chr4.1:28554504-28554523 MS.gene003415:CDS 40.0%
GGAGAATCAAGGGAAGAAAA+TGG + chr4.1:28554230-28554249 MS.gene003415:CDS 40.0%
GGATTAACATAAACACGAGG+AGG - chr4.1:28553583-28553602 None:intergenic 40.0%
GGTCTACGTTGAGAAAAAAG+AGG - chr4.1:28553754-28553773 None:intergenic 40.0%
GTGGGATTAACATAAACACG+AGG - chr4.1:28553586-28553605 None:intergenic 40.0%
GTTAGGGTTAGGATTAGGAT+TGG - chr4.1:28553782-28553801 None:intergenic 40.0%
TTAGGGTTAGGGTTAGGATT+AGG - chr4.1:28553787-28553806 None:intergenic 40.0%
TTCCTCCTAAAAACCTCAGT+AGG - chr4.1:28553988-28554007 None:intergenic 40.0%
TTGGAACTAATCCTCCATCT+GGG + chr4.1:28553857-28553876 MS.gene003415:CDS 40.0%
! AGGATCCTACTGAGGTTTTT+AGG + chr4.1:28553980-28553999 MS.gene003415:CDS 40.0%
! ATCAGTATTTGAGGAGTGTG+AGG + chr4.1:28554397-28554416 MS.gene003415:CDS 40.0%
! ATGAATACGGATGTTCTGGA+GGG + chr4.1:28554198-28554217 MS.gene003415:CDS 40.0%
! ATGTTGACGGATAAGCCATT+TGG - chr4.1:28553383-28553402 None:intergenic 40.0%
! CCCAATTGTTATCTGGCTTA+TGG + chr4.1:28553540-28553559 MS.gene003415:CDS 40.0%
! TCAGTATTTGAGGAGTGTGA+GGG + chr4.1:28554398-28554417 MS.gene003415:CDS 40.0%
! TTTGGAACTAATCCTCCATC+TGG + chr4.1:28553856-28553875 MS.gene003415:CDS 40.0%
!! ATCCTACTGAGGTTTTTAGG+AGG + chr4.1:28553983-28554002 MS.gene003415:CDS 40.0%
!! ATTTGGTGTCTTCGGAGTAA+GGG - chr4.1:28553366-28553385 None:intergenic 40.0%
!! GATAAGCCATTTGGTGTCTT+CGG - chr4.1:28553374-28553393 None:intergenic 40.0%
!! TAGATAAGGGTGTTCAGGTT+GGG + chr4.1:28554361-28554380 MS.gene003415:CDS 40.0%
!! TGCTTTAGATAAGGGTGTTC+AGG + chr4.1:28554356-28554375 MS.gene003415:CDS 40.0%
!! TTAGATAAGGGTGTTCAGGT+TGG + chr4.1:28554360-28554379 MS.gene003415:CDS 40.0%
!!! AGGGAGGAGGTTTTGAATAA+TGG + chr4.1:28554117-28554136 MS.gene003415:CDS 40.0%
AATTGTTGGGTTTCGGGGTT+TGG - chr4.1:28553304-28553323 None:intergenic 45.0%
AGGGAAGAAAATGGGGAGTT+TGG + chr4.1:28554239-28554258 MS.gene003415:CDS 45.0%
ATAATGATGTGACGGCGTTG+AGG + chr4.1:28554037-28554056 MS.gene003415:CDS 45.0%
ATTGTTGGGTTTCGGGGTTT+GGG - chr4.1:28553303-28553322 None:intergenic 45.0%
CCAGGATGAATATACCCAGA+TGG - chr4.1:28553874-28553893 None:intergenic 45.0%
CCATCTGGGTATATTCATCC+TGG + chr4.1:28553871-28553890 MS.gene003415:CDS 45.0%
CTAGAGAAGGTGAAATGGAG+GGG + chr4.1:28554064-28554083 MS.gene003415:CDS 45.0%
CTGAGGAATTGTTGGGTTTC+GGG - chr4.1:28553310-28553329 None:intergenic 45.0%
CTGGGTATATTCATCCTGGT+AGG + chr4.1:28553875-28553894 MS.gene003415:CDS 45.0%
CTTACTCCGAAGACACCAAA+TGG + chr4.1:28553365-28553384 MS.gene003415:CDS 45.0%
GAAGGAGATGCAAGATGAGA+TGG + chr4.1:28554143-28554162 MS.gene003415:CDS 45.0%
GACTAGAGAAGGTGAAATGG+AGG + chr4.1:28554062-28554081 MS.gene003415:CDS 45.0%
GAGATGCAAGATGAGATGGA+GGG + chr4.1:28554147-28554166 MS.gene003415:CDS 45.0%
GAGTTTGGAGAATGTTGAGG+AGG + chr4.1:28554254-28554273 MS.gene003415:CDS 45.0%
GATGAATACGGATGTTCTGG+AGG + chr4.1:28554197-28554216 MS.gene003415:CDS 45.0%
GCTGAGGAATTGTTGGGTTT+CGG - chr4.1:28553311-28553330 None:intergenic 45.0%
GGAACAGAAACCAATCCAGA+AGG - chr4.1:28553649-28553668 None:intergenic 45.0%
GGATGAATATACCCAGATGG+AGG - chr4.1:28553871-28553890 None:intergenic 45.0%
GGATTGAGCTGAGGAATTGT+TGG - chr4.1:28553318-28553337 None:intergenic 45.0%
GTTGGTTGAGGGAGAATCAA+GGG + chr4.1:28554220-28554239 MS.gene003415:CDS 45.0%
TAACTTTAGCAGCCGTAGCA+CGG - chr4.1:28554449-28554468 None:intergenic 45.0%
TGAGGAATTGTTGGGTTTCG+GGG - chr4.1:28553309-28553328 None:intergenic 45.0%
TGAGGATTAGGGTTAGGGTT+AGG - chr4.1:28553793-28553812 None:intergenic 45.0%
!! AGATAAGGGTGTTCAGGTTG+GGG + chr4.1:28554362-28554381 MS.gene003415:CDS 45.0%
!! AGGGGTTGTTTGGTTTGCAA+GGG + chr4.1:28554082-28554101 MS.gene003415:CDS 45.0%
!! ATCGGCTTGAAGGAGATTGA+CGG - chr4.1:28553479-28553498 None:intergenic 45.0%
!! CATTTGGTGTCTTCGGAGTA+AGG - chr4.1:28553367-28553386 None:intergenic 45.0%
!! GGATGTTGTATGTGACGGAT+TGG - chr4.1:28553522-28553541 None:intergenic 45.0%
!! GTTGTATGTGACGGATTGGT+AGG - chr4.1:28553518-28553537 None:intergenic 45.0%
AGGTTGCGAATATGGCTGCT+AGG + chr4.1:28554490-28554509 MS.gene003415:CDS 50.0%
AGTTAGAGCTGCTCAGTTGC+AGG + chr4.1:28554464-28554483 MS.gene003415:CDS 50.0%
CTTGAAGGAGATTGACGGCA+CGG - chr4.1:28553474-28553493 None:intergenic 50.0%
GCAAGATGAGATGGAGGGTT+TGG + chr4.1:28554152-28554171 MS.gene003415:CDS 50.0%
GCGTTGAGGAAGACTAGAGA+AGG + chr4.1:28554051-28554070 MS.gene003415:CDS 50.0%
GGAGAGAGAGGAGATGTTGA+CGG - chr4.1:28553396-28553415 None:intergenic 50.0%
GGAGATGCAAGATGAGATGG+AGG + chr4.1:28554146-28554165 MS.gene003415:CDS 50.0%
GGGGAGTTTGGAGAATGTTG+AGG + chr4.1:28554251-28554270 MS.gene003415:CDS 50.0%
GGTGAAATGGAGGGGTTGTT+TGG + chr4.1:28554072-28554091 MS.gene003415:CDS 50.0%
GTCAATCTCCTTCAAGCCGA+TGG + chr4.1:28553478-28553497 MS.gene003415:CDS 50.0%
TTGTTGGGTTTCGGGGTTTG+GGG - chr4.1:28553302-28553321 None:intergenic 50.0%
! GGTTGGTTGAGGGAGAATCA+AGG + chr4.1:28554219-28554238 MS.gene003415:CDS 50.0%
! TTTGGAGCAGCAGTTGCAGA+TGG + chr4.1:28554170-28554189 MS.gene003415:CDS 50.0%
! TTTGGGTTCGAGAGAAGGGA+AGG - chr4.1:28553425-28553444 None:intergenic 50.0%
!! ATACGGATGTTCTGGAGGGT+TGG + chr4.1:28554202-28554221 MS.gene003415:CDS 50.0%
!! ATGTTCTGGAGGGTTGGTTG+AGG + chr4.1:28554208-28554227 MS.gene003415:CDS 50.0%
!! GAGGGGTTGTTTGGTTTGCA+AGG + chr4.1:28554081-28554100 MS.gene003415:CDS 50.0%
!! GCAAGGGGTATTGAAGCAGA+GGG + chr4.1:28554098-28554117 MS.gene003415:CDS 50.0%
!! GGGGTTGTTTGGTTTGCAAG+GGG + chr4.1:28554083-28554102 MS.gene003415:CDS 50.0%
!! GTGACGGATTGGTAGGTCAT+GGG - chr4.1:28553511-28553530 None:intergenic 50.0%
!! TGACGGATTGGTAGGTCATG+GGG - chr4.1:28553510-28553529 None:intergenic 50.0%
!! TGAGGAGGCTTTTGAGTGTG+TGG + chr4.1:28554269-28554288 MS.gene003415:CDS 50.0%
!! TGAGTGTAGGTGGCGGTTTT+GGG - chr4.1:28553442-28553461 None:intergenic 50.0%
!! TGCAAGGGGTATTGAAGCAG+AGG + chr4.1:28554097-28554116 MS.gene003415:CDS 50.0%
!! TGTGACGGATTGGTAGGTCA+TGG - chr4.1:28553512-28553531 None:intergenic 50.0%
!! TGTTCTGGAGGGTTGGTTGA+GGG + chr4.1:28554209-28554228 MS.gene003415:CDS 50.0%
!!! CGGTTTTGGGTTCGAGAGAA+GGG - chr4.1:28553429-28553448 None:intergenic 50.0%
AAACACGAGGAGGGTGACGA+GGG - chr4.1:28553573-28553592 None:intergenic 55.0%
AGCTCAATCCTCTCCCAACG+CGG + chr4.1:28553328-28553347 MS.gene003415:CDS 55.0%
CTTATTCCCCTGCGCCATCT+CGG + chr4.1:28553944-28553963 MS.gene003415:CDS 55.0%
GCAGTTCTTCCACCGTGCTA+CGG + chr4.1:28554434-28554453 MS.gene003415:CDS 55.0%
GCATACGGAGGATCCTACTG+AGG + chr4.1:28553972-28553991 MS.gene003415:CDS 55.0%
GCCACCTACACTCACAACGA+CGG + chr4.1:28553448-28553467 MS.gene003415:CDS 55.0%
GGTCATGGGGATTGTGCCAT+CGG - chr4.1:28553497-28553516 None:intergenic 55.0%
GTAGGATCCTCCGTATGCGT+AGG - chr4.1:28553970-28553989 None:intergenic 55.0%
GTCGTTGTGAGTGTAGGTGG+CGG - chr4.1:28553449-28553468 None:intergenic 55.0%
GTCTTCGGAGTAAGGGAGAG+CGG - chr4.1:28553359-28553378 None:intergenic 55.0%
GTTGGGAGAGGATTGAGCTG+AGG - chr4.1:28553327-28553346 None:intergenic 55.0%
TAAACACGAGGAGGGTGACG+AGG - chr4.1:28553574-28553593 None:intergenic 55.0%
TGTTGGGTTTCGGGGTTTGG+GGG - chr4.1:28553301-28553320 None:intergenic 55.0%
! GCAGGCTCAGGTTGCGAATA+TGG + chr4.1:28554482-28554501 MS.gene003415:CDS 55.0%
! GGTATTGAAGCAGAGGGAGG+AGG + chr4.1:28554104-28554123 MS.gene003415:CDS 55.0%
!! AGGGGTATTGAAGCAGAGGG+AGG + chr4.1:28554101-28554120 MS.gene003415:CDS 55.0%
!! GGATTGTGCCATCGGCTTGA+AGG - chr4.1:28553489-28553508 None:intergenic 55.0%
!! GTGAGTGTAGGTGGCGGTTT+TGG - chr4.1:28553443-28553462 None:intergenic 55.0%
!!! GCGGTTTTGGGTTCGAGAGA+AGG - chr4.1:28553430-28553449 None:intergenic 55.0%
ACGGCCGTCGTTGTGAGTGT+AGG - chr4.1:28553455-28553474 None:intergenic 60.0%
AGCTGCTCAGTTGCAGGCTC+AGG + chr4.1:28554470-28554489 MS.gene003415:CDS 60.0%
ATCTCGGCCTACGCATACGG+AGG + chr4.1:28553960-28553979 MS.gene003415:CDS 60.0%
CGAGAGAAGGGAAGGTGGTG+AGG - chr4.1:28553417-28553436 None:intergenic 60.0%
CGAGATGGCGCAGGGGAATA+AGG - chr4.1:28553946-28553965 None:intergenic 60.0%
CTTTAGCAGCCGTAGCACGG+TGG - chr4.1:28554446-28554465 None:intergenic 60.0%
GCAGGGGAATAAGGCGAAGG+AGG - chr4.1:28553937-28553956 None:intergenic 60.0%
GCCATCTCGGCCTACGCATA+CGG + chr4.1:28553957-28553976 MS.gene003415:CDS 60.0%
GCCGTCGTTGTGAGTGTAGG+TGG - chr4.1:28553452-28553471 None:intergenic 60.0%
GGAAGGTGGTGAGGAGAGAG+AGG - chr4.1:28553408-28553427 None:intergenic 60.0%
GGGTTCGAGAGAAGGGAAGG+TGG - chr4.1:28553422-28553441 None:intergenic 60.0%
TCCGTATGCGTAGGCCGAGA+TGG - chr4.1:28553961-28553980 None:intergenic 60.0%
TCGGAGTAAGGGAGAGCGGA+GGG - chr4.1:28553355-28553374 None:intergenic 60.0%
TTCGGAGTAAGGGAGAGCGG+AGG - chr4.1:28553356-28553375 None:intergenic 60.0%
!! GAGTGTGAGGGCGTTGTCGA+GGG + chr4.1:28554410-28554429 MS.gene003415:CDS 60.0%
CGGAGTAAGGGAGAGCGGAG+GGG - chr4.1:28553354-28553373 None:intergenic 65.0%
GGCGCAGGGGAATAAGGCGA+AGG - chr4.1:28553940-28553959 None:intergenic 65.0%
GTTTCGGGGTTTGGGGGTGG+TGG - chr4.1:28553295-28553314 None:intergenic 65.0%
TGGGTTTCGGGGTTTGGGGG+TGG - chr4.1:28553298-28553317 None:intergenic 65.0%
!! GGAGTGTGAGGGCGTTGTCG+AGG + chr4.1:28554409-28554428 MS.gene003415:CDS 65.0%
CGTAGGCCGAGATGGCGCAG+GGG - chr4.1:28553953-28553972 None:intergenic 70.0%
GCGTAGGCCGAGATGGCGCA+GGG - chr4.1:28553954-28553973 None:intergenic 70.0%
TGCGTAGGCCGAGATGGCGC+AGG - chr4.1:28553955-28553974 None:intergenic 70.0%
GAGAGCGGAGGGGCCGCGTT+GGG - chr4.1:28553344-28553363 None:intergenic 75.0%
! CGGAGGGGCCGCGTTGGGAG+AGG - chr4.1:28553339-28553358 None:intergenic 80.0%
! GGAGAGCGGAGGGGCCGCGT+TGG - chr4.1:28553345-28553364 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 28553286 28554533 28553286 ID=MS.gene003415
chr4.1 mRNA 28553286 28554533 28553286 ID=MS.gene003415.t1;Parent=MS.gene003415
chr4.1 exon 28553286 28554533 28553286 ID=MS.gene003415.t1.exon1;Parent=MS.gene003415.t1
chr4.1 CDS 28553286 28554533 28553286 ID=cds.MS.gene003415.t1;Parent=MS.gene003415.t1
Gene Sequence

>MS.gene003415

ATGGTTCCACCACCCCCAAACCCCGAAACCCAACAATTCCTCAGCTCAATCCTCTCCCAACGCGGCCCCTCCGCTCTCCCTTACTCCGAAGACACCAAATGGCTTATCCGTCAACATCTCCTCTCTCTCCTCACCACCTTCCCTTCTCTCGAACCCAAAACCGCCACCTACACTCACAACGACGGCCGTGCCGTCAATCTCCTTCAAGCCGATGGCACAATCCCCATGACCTACCAATCCGTCACATACAACATCCCAATTGTTATCTGGCTTATGGAATCTTACCCTCGTCACCCTCCTCGTGTTTATGTTAATCCCACTAGGGATATGATTATTAAACATGCTCATCCTCATGTTAATCCTTCTGGATTGGTTTCTGTTCCGTATTTGCATAACTGGATTTACCCTAGTTCTAATTTGGTTGATCTTGTTCTTGCTTTGAGTTTGATTTTTGGTCGTGATCCACCTCTTTTTTCTCAACGTAGACCTAATCCCAATCCTAATCCTAACCCTAACCCTAATCCTCATCAAAATCATAATCCTAATCCAAATACCAATCAAAGTTCTAATTTTGGAACTAATCCTCCATCTGGGTATATTCATCCTGGTAGGATTAATAGTAATAGTAATATTAATATTAATAGTTATCCTCCTTCGCCTTATTCCCCTGCGCCATCTCGGCCTACGCATACGGAGGATCCTACTGAGGTTTTTAGGAGGAATGCTATTAACAAGCTTGTGGAGATGGTGCATAATGATGTGACGGCGTTGAGGAAGACTAGAGAAGGTGAAATGGAGGGGTTGTTTGGTTTGCAAGGGGTATTGAAGCAGAGGGAGGAGGTTTTGAATAATGGTGTGAAGGAGATGCAAGATGAGATGGAGGGTTTGGAGCAGCAGTTGCAGATGGTTTTGATGAATACGGATGTTCTGGAGGGTTGGTTGAGGGAGAATCAAGGGAAGAAAATGGGGAGTTTGGAGAATGTTGAGGAGGCTTTTGAGTGTGTGGATGTGCTTTCCAAACAGATGCTTGATTGTACTTCTGCTGATTTGGCTATTGAAGATACGCTTTATGCTTTAGATAAGGGTGTTCAGGTTGGGGCTGTACCGTTTGATCAGTATTTGAGGAGTGTGAGGGCGTTGTCGAGGGAGCAGTTCTTCCACCGTGCTACGGCTGCTAAAGTTAGAGCTGCTCAGTTGCAGGCTCAGGTTGCGAATATGGCTGCTAGGAATCATCATTATGGTAGCTGA
Protein sequence

>MS.gene003415.t1

MVPPPPNPETQQFLSSILSQRGPSALPYSEDTKWLIRQHLLSLLTTFPSLEPKTATYTHNDGRAVNLLQADGTIPMTYQSVTYNIPIVIWLMESYPRHPPRVYVNPTRDMIIKHAHPHVNPSGLVSVPYLHNWIYPSSNLVDLVLALSLIFGRDPPLFSQRRPNPNPNPNPNPNPHQNHNPNPNTNQSSNFGTNPPSGYIHPGRINSNSNININSYPPSPYSPAPSRPTHTEDPTEVFRRNAINKLVEMVHNDVTALRKTREGEMEGLFGLQGVLKQREEVLNNGVKEMQDEMEGLEQQLQMVLMNTDVLEGWLRENQGKKMGSLENVEEAFECVDVLSKQMLDCTSADLAIEDTLYALDKGVQVGAVPFDQYLRSVRALSREQFFHRATAAKVRAAQLQAQVANMAARNHHYGS